CN102701874B - Growing medium special for leafy organic vegetables and preparation method thereof - Google Patents
Growing medium special for leafy organic vegetables and preparation method thereof Download PDFInfo
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- CN102701874B CN102701874B CN201210231551XA CN201210231551A CN102701874B CN 102701874 B CN102701874 B CN 102701874B CN 201210231551X A CN201210231551X A CN 201210231551XA CN 201210231551 A CN201210231551 A CN 201210231551A CN 102701874 B CN102701874 B CN 102701874B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides a growing medium special for leafy organic vegetables and a preparation method thereof. The growing medium is prepared from furfural residue, an oil residue, a smoke residue, a microbial fermentation fungicide, crop straw ash, an azotobacter fungicide and roseite, wherein the azotobacter fungicide refers to liquid fungicide prepared by cultivating and fermenting azotobacteria; and the liquid contains 4x10<8>-7x10<8> viable bacteria per gram. The growing medium is prepared by the steps of: adding the furfural residue, the oil residue and the smoke residue in the microbial fermentation fungicide and stacking for fermenting and composting, adding the crop straw ash and the azotobacter fungicide and stacking for 3-5 days and finally adding the roseite, and evenly mixing. The growing medium contains a great amount of active azotobacter for fixing nitrogen in air, so that more nitrogen nutrition can be provided for vegetables, the nutrition balance is promoted, the yield of vegetables is enhanced and the quality is improved.
Description
Technical field
The present invention relates to a kind of arable farming matrix, particularly a kind of leaf class organic vegetable plantation substrate and preparation method thereof, belong to crop cultivation technique without soil field.
Background technology
Along with growth in the living standard, organic farm products more and more is subject to people's favor.So-called organic farm products, finger is from chemical substances such as Organic farming production system, the agricultural chemicals that does not use any harmful chemosynthesis in the place of production of eco-environmental quality standard up to specification, production process, chemical fertilizer, according to international Organic farming production requirement and corresponding standard production and through organic farm products certification authority independently, authenticate for the edible product of the mankind.
In view of the restriction of the strict working condition of organic farm products with have vegetable plot now and use in a large number the disadvantageous effect that Soil structure destruction that chemical fertilizer, agricultural chemicals etc. bring, soil compaction, soil fertility decline etc. bring to organic farm products high yield, stable yields and cleaner production, utilizing soilless culture is to guarantee one of important channel of organic farm products supply.
Leafy vegetable is the popular vegetables that people generally eat, and the current consumption amount is large, fast growth, and growth cycle is short, is particularly suitable for carrying out soilless culture.The edible part of leafy vegetable is stem, leaf, therefore, with common crop, compares, and growing of leafy vegetable needs to absorb relatively many nitrogen nutrition.Common cultivation matrix can't meet the absorption requirement of leafy vegetable to nitrogen.
The basis of soilless culture is cultivation matrix, according to the organic farming requirement, in matrix, can not add chemical nitrogen fertilizer.Common cultivation matrix not only absolute magnitude can not meet the requirement of leafy vegetable nitrogen, has the imbalance between nutrient simultaneously, is difficult to guarantee vegetables high yield, high-quality.
Vinelandii can be under normal temperature and pressure conditions, and the effect by nitrogenase in its body, be fixed up airborne nitrogen, forms the nitrogen that plant can absorb.It is the good natural mediums of vinelandii that cultivation matrix particularly be take the cultivation matrix that organism is main component, if vinelandii are added in cultivation matrix and can obviously increase available nitrogen content in matrix, promote the nutrition supply balance, improve the yield and quality of leafy vegetable, meet the organic farming requirement simultaneously.Have no at present relevant report.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of leaf class organic vegetable plantation substrate and preparation method thereof.
It is composite that the present invention utilizes different organic wastes to carry out, sterilizing after fermentation maturity, then add vinelandii microbial inoculum, composting to obtain leaf class organic vegetable plantation substrate.
The term explanation:
Leafy vegetable: referring to eat cauline leaf is main vegetables, as Chinese cabbage, cabbage, spinach, celery, rape, caraway (coriander), romaine lettuce, Caulis et Folium Ipomoeae aquaticae, Herba basellae rubrae, shepherd's purse, three-coloured amaranth, crowndaisy chrysanthemum, fennel, leek, garlic bolt etc.
Vinelandii: be that a class can absorb nitrogen as nitrogenous source and convert it into the microorganism that crop can utilize nitrogen form from air, mainly be divided into azotobacter and symbiotic nitrogen-fixing bacteria.Azotobacter as azotobacter chroococcum etc. can utilize airborne nitrogen for own Growth and reproduction offspring, after death, nitrogen can be contributed to plant.Symbiotic nitrogen-fixing bacteria be only in the situation that with plant symbiosis the nitrogen in could fixed air, fixing nitrogen can directly provide nitrogenous source for symbiote, as root nodule bacterium.The present invention utilizes azotobacter.
Technical scheme of the present invention is as follows:
Leaf class organic vegetable plantation substrate, raw materials quality part is as follows:
250～400 parts of furfural dregs,
50～120 parts of the dregs of fat,
180～300 parts of scarps,
1～3 part of microorganism fermenting agent,
20～40 parts of crop straw ashes,
2～6 parts of vinelandii microbial inoculums,
280～350 parts of vermiculites.
Described vinelandii microbial inoculum is liquid microbe microbial inoculum prepared through steps such as cultivation, fermentations by prior art by azotobacter.Bacterium liquid is containing viable count 4 * 10
8individual/gram～7 * 10
8individual/gram.
The vinelandii bacterial classification is selected from blown-ball Azotobacter (Azotobacter chrooccum), Wei Nielande vinelandii (Azotobacter vinelandii), vinelandii (Azotobacter sp.).Wherein preferably blown-ball Azotobacter ACCC01077 or the ACCC11104 of Chinese agriculture microbial strains preservation administrative center (Agricultural Culture Collection of China english abbreviation ACCC).
According to the present invention, preferred, described vinelandii microbial inoculum makes by the following method:
Solid culture based formulas: sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, agar 18 grams, water 1000 grams, pH7.0～7.5.Under 0.11 MPa pressure, sterilizing makes test tube slant in 30 minutes.Azotobacter bacterial classification under aseptic condition, be placed in 28 ℃ of constant incubators and cultivate 5～7 days.Obtain vinelandii inclined-plane seed.
Liquid culture based formulas: sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, water 1000 grams, pH7.0～7.5.1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks, by the prior art sterilizing, three, access vinelandii inclined-plane seed in each triangular flask after cooling, 3～5 millimeters of every diameters are cultivated 4 days under 28 ℃～30 ℃, 150 rev/mins conditions.Obtain the vinelandii liquid bacterial agent.Bacterium liquid is containing viable count 4 * 10
8individual/gram～7 * 10
8individual/gram.
Described furfural dregs is that corn cob is that main raw material is manufactured the waste residue in the furfural process.
The described dregs of fat are that the plant oil pressing factory utilizes squeezing process to be pushed, come unstuck and produce remaining residue after edible oil raw material, comprise soya-bean oil slag, the peanut dregs of fat, the cottonseed dregs of fat or the Semen Brassicae campestris dregs of fat, can select one of them, also use capable of being combined.
Described scarp is tobacco leaf residue discarded in the production of cigarettes process.
Described microorganism fermenting agent is EM composite fungus agent (EM is the abbreviation of EffectiveMicroorganesms), and it is that 5 sections 10 such as photosynthetic bacteria group, yeast flora, lactobacillus, actinomycete group, mycorhiza flora are belonged to 80 multiple-microorganisms compound a kind of typing microbial inoculum of cultivating in same state.Be brown translucent liquid, the pH value is between 3.5～4.5.Market is on sale.
The waste residue of described crop straw ash under to be crop stalk residual after burning or alternate manner make organic substance decomposing, mainly utilizing composition is mineral substance contained in crop straw.Preferably, the crop straw ash is selected from the residue after corn stalk, wheat straw, soybean straws, paddy stalk, peanut straw or vegetables straw burn.
Described vermiculite particle diameter 2-4mm.Commercially available prod.
According to the present invention, preferred, leaf class organic vegetable plantation substrate raw materials quality part is as follows:
340～360 parts of furfural dregs,
95～105 parts of the dregs of fat,
190～210 parts of scarps,
1～2 part of microorganism fermenting agent,
24～26 parts of crop straw ashes,
4.5～5.5 parts of vinelandii microbial inoculums,
310～320 parts of vermiculites.
The preparation method of leaf class organic vegetable plantation substrate of the present invention, step is as follows:
(1) getting furfural dregs, the dregs of fat, scarp and microorganism fermenting agent by proportioning mixes.The moisture of adjusting compound is 55 ~ 60wt%, and fermentation reactor system becomes thoroughly decomposed, and temperature rises to 55 ℃ of timing, keeps 3 ~ 5 days.Turning, temperature rises to 50 ℃ of timing again, keeps 3 ~ 5 days.So operation repeats 2 ~ 3 times again, ferments altogether 18～40 days.Obtain fermentation material.This fermentation material is carried out to steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps 6～8 hours, obtains the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum:
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, agar 18 grams, water 1000 grams, regulate pH to 7.0～7.5.Under 0.11 MPa pressure, sterilizing makes test tube slant in 30 minutes.Azotobacter bacterial classification under aseptic condition, be placed in 28 ℃ of constant incubators and cultivate 5～7 days.Obtain vinelandii inclined-plane seed.
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, water 1000 grams, regulate pH to 7.0～7.5, makes liquid nutrient medium.1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks, by the prior art sterilizing, three, access vinelandii inclined-plane seed in each triangular flask after cooling, 3～5 millimeters of every diameters are cultivated 4 days under 28 ℃～30 ℃, 150 rev/mins conditions.Obtain the vinelandii liquid bacterial agent.Bacterium liquid is containing viable count 4 * 10
8individual/gram-7 * 10
8individual/gram.
(3) by proportioning, get in the sterilization fermentation material that the crop straw ash adds step (1) to prepare, the pH that regulates this fermentation material is 7.0～7.5,2～6 parts of the vinelandii liquid bacterial agents that adds again step (2) to prepare, mix, the moisture of adjusting compound is 55 ~ 60wt%, and under 28 ℃～30 ℃ conditions, composting is 3～5 days, must contain the mixture of vinelandii.
(4) by proportioning, in the mixture that contains vinelandii that adds step (3) to prepare vermiculite, mix, dry in the air to moisture content 27～30wt%, obtain cultivation matrix of the present invention.
Preferably, in step (2), the vinelandii bacterial classification is selected from blown-ball Azotobacter ACCC01077 or ACCC11104.
The application method of leaf class organic vegetable plantation substrate of the present invention:
By this cultivation matrix cultivating container (basin or geosyncline etc.) of packing into, thickness is 13～20 centimetres, sows according to a conventional method vegetable seed or the seedling of planting, and control measures are pressed the common cultivation matrix technology.
Cultivation matrix of the present invention contains a large amount of active vinelandii, and they can continue breeding in the vegetable growing process in matrix, and then the nitrogen in fixed air continuously, for vegetables provide more nitrogen nutrition, and promoted to increase vegetable crop by nutrient balance, improve quality.The present invention, through overtesting, has obtained good effect.Excellent results of the present invention is as follows:
1. nitrogen content in increase matrix
Leaf class organic vegetable plantation substrate of the present invention be take organic substance as main, the good culture materials of vinelandii, in the vegetable growth process, the conditions such as its moisture, temperature, ventilation also are applicable to the vinelandii breeding very much, therefore nitrogen in fixed air constantly, increase the nitrogen content in matrix.
2. promote cultivation matrix nutrient supply balance
The more nitrogen nutrition of good growth needs of leafy vegetable, nitrogen content deficiency in common cultivation matrix, make the Major Nutrients such as nitrogen, phosphorus, potassium can not the balance supply.In matrix, constantly the vinelandii of breeding can be under normal temperature and pressure conditions, and the effect by nitrogenase in its body, be fixed up airborne nitrogen, forms the nitrogen that plant can absorb, and promotes nutrient supply balance in matrix.
3. raising vegetable crop
Nutrient supply abundance and balance are the bases of the good growth of leafy vegetable and high yield, and comparing leafy vegetable with general crop needs more nitrogen nutrition, and common cultivation matrix can not meet the demands.The present invention, by adding vinelandii in matrix, has increased nitrogen content in cultivation matrix, has promoted the balance between nutrient, has improved leafy vegetable output.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, but be not limited to this.In embodiment, ratio, content are mass ratio.Raw material is described as follows:
The vinelandii bacterial classification is: blown-ball Azotobacter ACCC01077 or ACCC11104, and Chinese agriculture microbial strains preservation administrative center buys, wherein, embodiment 1-2 ACCC01077, embodiment 3-4 ACCC11104.
Furfural dregs, Henan Hongye Chemical Industry Co., Ltd take corn cob and manufactures the waste residue in the furfural process as main raw material.
Scarp, discarded tobacco leaf waste residue in China Tobacco Henan Industrial Corporation's production process.
The dregs of fat are remaining residue after Shandong wide life limited-liability company production edible oil, are the combinations with 1: 1 mass ratio of the soya-bean oil dregs of fat and the peanut oil dregs of fat.
The microorganism fermenting agent is the EM composite fungus agent, and Jiangxi Tianyi Biologic Technology Development Co., Ltd. produces.
The crop straw ash is the corn stalk ash, and Heze city of Shandong province is with sharp biotechnology company limited byproduct.
Vermiculite, source, Lingshou County, Hebei province permanent vermiculite source mill, particle diameter 2-4mm.
Embodiment 1:
Leaf class organic vegetable plantation substrate, material component is as follows:
Furfural dregs 400kg,
Dregs of fat 50kg,
Scarp 210kg,
Microorganism fermenting agent 3kg,
Crop straw ash 35kg,
Vinelandii microbial inoculum 2kg,
Vermiculite 300kg.
The preparation method:
(1) get 400 kilograms of furfural dregs, 50 kilograms of the dregs of fat, 210 kilograms of scarps, 3 kilograms of microorganism fermenting agents, mix.The moisture of adjusting compound is 55wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 4 days.Turning, temperature rises to 50 ℃ of timing again, keeps 3 days.So operation repeats 3 times again, ferments altogether 28 days.Obtain fermentation material.This fermentation material is carried out to steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps 6 hours, obtains the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum:
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, agar 18 grams, water 1000 grams, regulate pH7.3.Under 0.11 MPa pressure, sterilizing makes test tube slant in 30 minutes.After inclined-plane has made under aseptic condition Azotobacter bacterial classification ACCC01077, be placed in 28 ℃ of constant incubators and cultivate 6 days.Obtain vinelandii inclined-plane seed.
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, water 1000 grams, regulate pH7.2, obtains liquid nutrient medium.1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks, by the prior art sterilizing, cooling after three, access vinelandii inclined-plane seed in each triangular flask, 3 millimeters of every diameters are cultivated 4 days under 28 ℃, 150 rev/mins conditions.Obtain the vinelandii liquid bacterial agent.Bacterium liquid is containing viable count 4.38 * 10
8individual/gram.
(3) get in 35 kilograms of sterilization fermentation material that add step (1) to prepare of crop straw ash, the pH that regulates this fermentation material is 7.4,2 kilograms of the vinelandii liquid bacterial agents that adds again step (2) to prepare, mix, the moisture of adjusting compound is 60wt%, under 28 ℃ of conditions, composting is 4 days, must contain the mixture of vinelandii.
(4) get 300 kilograms of vermiculites, in the mixture that contains vinelandii that adds step (3) to prepare, mix, dry in the air to moisture content 28%, obtain cultivation matrix of the present invention.
Embodiment 2:
Leaf class organic vegetable plantation substrate, material component is as follows:
Furfural dregs 300kg,
Dregs of fat 70kg,
Scarp 255kg,
Microorganism fermenting agent 2kg,
Crop straw ash 20kg,
Vinelandii microbial inoculum 3kg,
Vermiculite 350kg.
The preparation method:
(1) get 300 kilograms of furfural dregs, 70 kilograms of the dregs of fat, 255 kilograms of scarps, 2 kilograms of microorganism fermenting agents, mix.The moisture of adjusting compound is 60wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 5 days.Turning, temperature rises to 50 ℃ of timing again, keeps 3 days.So operation repeats 2 times again, ferments altogether 24 days.Obtain fermentation material.This fermentation material is carried out to steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps 8 hours, obtains the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum is with embodiment 1.
(3) get in 20 kilograms of sterilization fermentation material that add step (1) to prepare of crop straw ash, the pH that regulates this fermentation material is 7.1,3 kilograms of the vinelandii liquid bacterial agents that adds again step (2) to prepare, mix, the moisture of adjusting compound is 55wt%, under 30 ℃ of conditions, composting is 3 days, must contain the mixture of vinelandii.
(4) get 350 kilograms of vermiculites, in the mixture that contains vinelandii that adds step (3) to prepare, mix, dry in the air to moisture content 30%, obtain cultivation matrix of the present invention.
Embodiment 3:
Leaf class organic vegetable plantation substrate, material component is as follows:
Furfural dregs 350kg,
Dregs of fat 100kg,
Scarp 200kg,
Microorganism fermenting agent 1kg,
Crop straw ash 25kg,
Vinelandii microbial inoculum 5kg,
Vermiculite 319kg.
The preparation method:
(1) get 350 kilograms of furfural dregs, 100 kilograms of the dregs of fat, 200 kilograms of scarps, 1 kilogram of microorganism fermenting agent, mix.The moisture of adjusting compound is 58wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 4 days.Turning, temperature rises to 50 ℃ of timing again, keeps 4 days.So operation repeats 2 times again, ferments altogether 24 days.Obtain fermentation material.This fermentation material is carried out to steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps 7 hours, obtains the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum is with embodiment 1, and difference is that the vinelandii bacterial classification adopts blown-ball Azotobacter ACCC11104, and the effective bacterial content of bacterium liquid is 5.96 * 10
8individual/gram.
(3) get in 25 kilograms of sterilization fermentation material that add step (1) to prepare of crop straw ash, the pH that regulates this fermentation material is 7.2,5 kilograms of the vinelandii liquid bacterial agents that adds again step (2) to prepare, mix, the moisture of adjusting compound is 55wt%, under 30 ℃ of conditions, composting is 4 days, must contain the mixture of vinelandii.
(4) get 319 kilograms of vermiculites, in the mixture that contains vinelandii that adds step (3) to prepare, mix, dry in the air to moisture content 29%, obtain cultivation matrix of the present invention.
Embodiment 4:
Leaf class organic vegetable plantation substrate, material component is as follows:
Furfural dregs 250kg,
Dregs of fat 120kg,
Scarp 300kg,
Microorganism fermenting agent 2kg,
Crop straw ash 40kg,
Vinelandii microbial inoculum 4kg,
Vermiculite 284kg.
The preparation method:
(1) get 250 kilograms of furfural dregs, dregs of fat 120 kg, 300 kilograms of scarps, 2 kilograms of microorganism fermenting agents, mix.The moisture of adjusting compound is 58wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 5 days.Turning, temperature rises to 50 ℃ of timing again, keeps 5 days.So operation repeats 3 times again, ferments altogether 40 days.Obtain fermentation material.This fermentation material is carried out to steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps 7 hours, obtains the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum is with embodiment 1, and difference is that the vinelandii bacterial classification adopts blown-ball Azotobacter ACCC11104, and the effective bacterial content of bacterium liquid is 5.96 * 10
8individual/gram.
(3) get in 40 kilograms of sterilization fermentation material that add step (1) to prepare of crop straw ash, the pH that regulates this fermentation material is 7.5,4 kilograms of the vinelandii liquid bacterial agents that adds again step (2) to prepare, mix, the moisture of adjusting compound is 58wt%, under 28 ℃ of conditions, composting is 5 days, must contain the mixture of vinelandii.
(4) get 284 kilograms of vermiculites, in the mixture that contains vinelandii that adds step (3) to prepare, mix, dry in the air to moisture content 27%, obtain cultivation matrix of the present invention.
Comparative Examples: the coventional type cultivation matrix, Shandong Guangda Fertilizer Technology Co., Ltd. produces, and is mainly that organism and the vermiculite become thoroughly decomposed mixes and make with the mass ratio ratio of 2: 1.Organic matter raw material is Klinit dregs and the furfural dregs composition with 1: 1 mass ratio.
The effect of above embodiment 1～4 and Comparative Examples is as follows:
Test site: the county that conquers east, Shandong Province, Shandong Guangda Fertilizer Technology Co., Ltd. test farm.
Test period: year April in January, 2012 to 2012.
The cultivation of employing geosyncline, 20 centimetres of groove depths, wide 40 centimetres, long 800 centimetres, the degree of depth of cultivation matrix in geosyncline is 16 centimetres, plants 3 row.
One, for studying thing, be celery, kind is that texts and pictures draw.Effect is in Table 1.
Table 1. is compared the effect of embodiment 1-4 with Comparative Examples
| Cultivation matrix of the present invention | Stimulation ratio % | Edible part crude protein improves % |
| Embodiment 1 | 8.3 | 0.12 |
| Embodiment 2 | 7.9 | 0.12 |
| Embodiment 3 | 10.2 | 0.08 |
| Embodiment 4 | 6.5 | 0.10 |
Two, for studying thing, be romaine lettuce, experimental cultivar is the glass romaine lettuce.Effect is in Table 2.
Table 2. is compared the effect of embodiment 1-4 cultivation matrix with Comparative Examples
| Cultivation matrix of the present invention | Stimulation ratio % | Edible part crude protein improves % |
| Embodiment 1 | 5.8 | 0.12 |
| Embodiment 2 | 6.6 | 0.11 |
| Embodiment 3 | 7.3 | 0.13 |
| Embodiment 4 | 6.9 | 0.13 |
Claims (1)
1. the preparation method of leaf class organic vegetable plantation substrate, raw materials quality part is as follows:
250～400 parts of furfural dregs,
50～120 parts of the dregs of fat,
180～300 parts of scarps,
1～3 part of microorganism fermenting agent,
20～40 parts of crop straw ashes,
2～6 parts of vinelandii microbial inoculums,
280～350 parts of vermiculites;
Described furfural dregs is that corn cob is that main raw material is manufactured the waste residue in the furfural process;
Described scarp is tobacco leaf residue discarded in the production of cigarettes process;
Described microorganism fermenting agent is the EM composite fungus agent;
Described vinelandii microbial inoculum is liquid microbe microbial inoculum prepared through steps such as cultivation, fermentations by prior art by azotobacter, and bacterium liquid is containing viable count 4 ╳ 10
8individual/gram～7 ╳ 10
8individual/gram;
The vinelandii bacterial classification be selected from blown-ball Azotobacter (
azotobacter chrooccum), the Wei Nielande vinelandii (
azotobacter vinelandii), vinelandii (
azotobacter sp.);
Step is as follows:
(1) by proportioning, get furfural dregs, the dregs of fat, scarp, the microorganism fermenting agent, mix; The moisture of adjusting compound is 55 ~ 60 wt %, and fermentation reactor system becomes thoroughly decomposed, and temperature rises to 55 ℃ of timing, keeps 3 ~ 5 days; Turning, temperature rises to 50 ℃ of timing again, keeps 3 ~ 5 days; So operation repeats 2 ~ 3 times again, ferments altogether 18～40 days; Obtain fermentation material; This fermentation material is carried out to steam sterilizing, pass into steam, temperature rises to 100 ℃ of timing, keeps 6～8 hours, obtains the sterilization fermentation material;
(2) preparation of vinelandii microbial inoculum
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, agar 18 grams, water 1000 grams, regulate pH to 7.0～7.5; Under 0.11 MPa pressure, sterilizing makes test tube slant in 30 minutes; Azotobacter bacterial classification under aseptic condition, be placed in 28 ℃ of constant incubators and cultivate 5～7 days; Obtain vinelandii inclined-plane seed;
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, magnesium sulfate heptahydrate 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, calcium carbonate 5 grams, water 1000 grams, regulate pH to 7.0～7.5, makes liquid nutrient medium; 1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks, by the prior art sterilizing, three, access vinelandii inclined-plane seed in each triangular flask after cooling, 3～5 millimeters of every diameters are cultivated 4 days under 28 ℃～30 ℃, 150 rev/mins conditions; Obtain the vinelandii liquid bacterial agent, bacterium liquid is containing viable count 4 ╳ 10
8individual/gram-7 ╳ 10
8individual/gram;
(3) by proportioning, get in the sterilization fermentation material that the crop straw ash adds step (1) to prepare, the pH that regulates this fermentation material is 7.0～7.5, the vinelandii liquid bacterial agent that adds again step (2) to prepare, mix, adjusting mixture moisture is 55 ~ 60 wt %, and under 28 ℃～30 ℃ conditions, composting is 3～5 days, must contain the mixture of vinelandii;
(4) by proportioning, in the mixture that contains vinelandii that adds step (3) to prepare vermiculite, mix, dry in the air to moisture content 27～30wt%, obtain.
2, the preparation method of leaf class organic vegetable plantation substrate according to claim 1 is characterized in that raw materials quality part is as follows:
340～360 parts of furfural dregs,
95～105 parts of the dregs of fat,
190～210 parts of scarps,
1～2 part of microorganism fermenting agent,
24～26 parts of crop straw ashes,
4.5～5.5 parts of vinelandii microbial inoculums,
310～320 parts of vermiculites.
3, the preparation method of leaf class organic vegetable plantation substrate according to claim 1, is characterized in that the vinelandii bacterial classification is selected from blown-ball Azotobacter ACCC01077 or ACCC11104.
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| CN104446879A (en) * | 2014-10-29 | 2015-03-25 | 宁夏共享生物化工有限公司 | Soilless culture medium for vegetables |
| CN104591900A (en) * | 2015-01-29 | 2015-05-06 | 孙丽君 | Potted vegetable nutrient soil and preparation method thereof |
| CN104973977A (en) * | 2015-07-09 | 2015-10-14 | 广西宏华生物实业股份有限公司 | Special matrix for organic vegetable soilless cultivation, and preparation method thereof |
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| KR100399401B1 (en) * | 2001-05-15 | 2003-09-26 | 심연숙 | A manufacturing method of organic fertilizer |
| CN101723764B (en) * | 2009-12-09 | 2011-01-19 | 山东光大肥业科技有限公司 | Special organic-inorganic compound fertilizer for Chinese chives and preparation method thereof |
| CN101734960B (en) * | 2009-12-18 | 2010-12-15 | 山东光大肥业科技有限公司 | Special organic fertilizer for leaf-vegetable organic vegetable and preparation method |
| CN102417423B (en) * | 2011-07-06 | 2014-02-12 | 山东宝源生物有限公司 | Nutritious compound fertilizer of swamp cabbage |
| CN102503605B (en) * | 2011-09-29 | 2013-10-30 | 山东光大肥业科技有限公司 | Biological organic fertilizer specially used for carthamus tinctorious L. and preparation method thereof |
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