CN102701874A - Growing medium special for leafy organic vegetables and preparation method thereof - Google Patents
Growing medium special for leafy organic vegetables and preparation method thereof Download PDFInfo
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- CN102701874A CN102701874A CN201210231551XA CN201210231551A CN102701874A CN 102701874 A CN102701874 A CN 102701874A CN 201210231551X A CN201210231551X A CN 201210231551XA CN 201210231551 A CN201210231551 A CN 201210231551A CN 102701874 A CN102701874 A CN 102701874A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a growing medium special for leafy organic vegetables and a preparation method thereof. The growing medium is prepared from furfural residue, an oil residue, a smoke residue, a microbial fermentation fungicide, crop straw ash, an azotobacter fungicide and roseite, wherein the azotobacter fungicide refers to liquid fungicide prepared by cultivating and fermenting azotobacteria; and the liquid contains 4x10<8>-7x10<8> viable bacteria per gram. The growing medium is prepared by the steps of: adding the furfural residue, the oil residue and the smoke residue in the microbial fermentation fungicide and stacking for fermenting and composting, adding the crop straw ash and the azotobacter fungicide and stacking for 3-5 days and finally adding the roseite, and evenly mixing. The growing medium contains a great amount of active azotobacter for fixing nitrogen in air, so that more nitrogen nutrition can be provided for vegetables, the nutrition balance is promoted, the yield of vegetables is enhanced and the quality is improved.
Description
Technical field
The present invention relates to a kind of arable farming matrix, special-purpose culture substrate of particularly a kind of leaf class organic vegetable and preparation method thereof belongs to crop cultivation technique without soil field.
Background technology
Along with growth in the living standard, organic farm products more and more receives people's favor.So-called organic farm products; Finger does not use chemical substances such as the agricultural chemicals, chemical fertilizer of any harmful chemosynthesis from the Organic farming production system, in the place of production of eco-environmental quality standard up to specification, production process, produce according to international Organic farming production requirement and corresponding standard, and through the product of the confession human consumption of organic farm products certification authority authentication independently.
In view of the strict working condition restriction of organic farm products with have the vegetable plot now and use the disadvantageous effect that Soil structure destruction that chemical fertilizer, agricultural chemicals etc. bring, soil compaction, soil fertility decline etc. bring for organic farm products high yield, stable yields and cleaner production in a large number, utilizing soilless culture is to guarantee one of important channel of organic farm products supply.
Leafy vegetable is the generally edible popular vegetables of people, and the current consumption amount is big, fast growth, and growth cycle is short, is particularly suitable for carrying out soilless culture.The edible part of leafy vegetable is stem, leaf, therefore, compares with common crop, and growing of leafy vegetable needs to absorb many relatively nitrogen nutrition.Common cultivation matrix can't satisfy leafy vegetable to the absorption of nitrogen requirement.
The basis of soilless culture is a culture substrate, according to the organic farming requirement, can not add chemical nitrogen fertilizer in the matrix.Common cultivation matrix not only absolute magnitude can not satisfy the requirement of leafy vegetable nitrogen, has the imbalance between the nutrient simultaneously, is difficult to guarantee vegetables high yield, high-quality.
Vinelandii can be under normal temperature and pressure conditions, and the effect through nitrogenase in its body is fixed up airborne nitrogen, forms the nitrogen that plant can absorb.Culture substrate particularly is that the culture substrate of staple is the good natural mediums of vinelandii with the organism; As if being added in the culture substrate, vinelandii then can obviously increase available nitrogen content in the matrix; Promote the nutrition supply balance; Improve the yield and quality of leafy vegetable, meet the organic farming requirement simultaneously.Do not see relevant report at present.
Summary of the invention
To the deficiency of prior art, the present invention provides special-purpose culture substrate of a kind of leaf class organic vegetable and preparation method thereof.
It is composite that the present invention utilizes different organic wastes to carry out, and sterilizes behind the fermentation maturity, adds the vinelandii microbial inoculum again, and composting promptly gets the special-purpose culture substrate of leaf class organic vegetable.
The term explanation:
Leafy vegetable: refer to that with edible cauline leaf be master's vegetables, like Chinese cabbage, cabbage, spinach, celery, rape, caraway (coriander), romaine lettuce, Caulis et Folium Ipomoeae aquaticae, Herba basellae rubrae, shepherd's purse, three-coloured amaranth, crowndaisy chrysanthemum, fennel, leek, garlic bolt etc.
Vinelandii: be that one type of ability absorbs nitrogen as nitrogenous source and convert it into the mikrobe of crop nitrogen form capable of using from air, mainly be divided into azotobacter and symbiotic nitrogen-fixing bacteria.Azotobacter such as azotobacter chroococcum etc. can utilize airborne nitrogen to supply oneself to grow and raise up seed, and can nitrogen be contributed to plant after the death.Symbiotic nitrogen-fixing bacteria be only with the situation of plant symbiosis under nitrogen in could fixed air, the fixed nitrogen can directly provide nitrogenous source for symbiote, like root nodule bacterium.The present invention utilizes azotobacter.
Technical scheme of the present invention is following:
The special-purpose culture substrate of leaf class organic vegetable, raw materials quality part is following:
250～400 parts of furfural dregs,
50～120 parts of the dregs of fat,
180～300 parts of scarps,
1～3 part of microbial fermentation microbial inoculum,
20～40 parts of crop straw ashes,
2～6 parts of vinelandii microbial inoculums,
280～350 parts of vermiculites.
Described vinelandii microbial inoculum is that azotobacter is by the liquid microbe microbial inoculum of prior art through the preparation of steps such as cultivation, fermentation.Bacterium liquid contains viable count 4 * 10
8Individual/gram～7 * 10
8Individual/gram.
The vinelandii bacterial classification is selected from blown-ball Azotobacter (Azotobacter chrooccum), Wei Nielande vinelandii (Azotobacter vinelandii), vinelandii (Azotobacter sp.).The blown-ball Azotobacter ACCC01077 or the ACCC11104 of wherein preferred Chinese agriculture microbial strains preservation administrative center (Agricultural Culture Collection of China english abbreviation ACCC).
According to the present invention, preferred, described vinelandii microbial inoculum makes by following method:
Solid culture based formulas: sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, agar 18 grams, water 1000 grams, pH7.0～7.5.Sterilization made the test tube slant in 30 minutes under 0.11 MPa pressure.Inoculation vinelandii bacterial classification is placed in 28 ℃ of constant incubators and cultivated 5～7 days under aseptic condition.Get vinelandii inclined-plane seed.
Liquid culture based formulas: sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, water 1000 grams, pH7.0～7.5.1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks; By the prior art sterilization; Insert three in vinelandii inclined-plane seed in each triangular flask of cooling back, 3～5 millimeters of every diameters were cultivated 4 days under 28 ℃～30 ℃, 150 rev/mins conditions.Get the vinelandii liquid bacterial agent.Bacterium liquid contains viable count 4 * 10
8Individual/gram～7 * 10
8Individual/gram.
Said furfural dregs is that corn cob is that main raw material is made the waste residue in the furfural process.
The described dregs of fat are that the plant oil pressing factory utilizes squeezing process that raw material is pushed, comes unstuck and produce remaining residue behind the edible oil, comprise soya-bean oil slag, the peanut dregs of fat, the cottonseed dregs of fat or the Semen Brassicae campestris dregs of fat, can select one of them for use, use also capable of being combined.
Described scarp is a depleted tobacco leaf residue in the production of cigarettes process.
Described microbial fermentation microbial inoculum is EM composite fungus agent (EM is the abbreviation of EffectiveMicroorganesms), and it is that 5 sections 10 such as photosynthetic bacteria group, yeast flora, lactobacillus, actinomycete group, mycorhiza flora are belonged to more than 80 kind of mikrobe compound a kind of typing microbial inoculum of cultivating in same state.Be brown translucent liquid, the pH value is between 3.5～4.5.Market is on sale.
To be crop stalk make organism decompose the waste residue of back under residual through burning or alternate manner to described crop straw ash, the mineral substance that mainly utilizes composition to be in the crop straw to be contained.Preferably, the crop straw ash is selected from the residue after corn stalk, wheat straw, soybean straw, paddy stalk, peanut straw or vegetables straw burn.
Described vermiculite particle diameter 2-4mm.The commercially available prod.
According to the present invention, preferred, the special-purpose culture substrate raw materials quality of leaf class organic vegetable part is following:
340～360 parts of furfural dregs,
95～105 parts of the dregs of fat,
190～210 parts of scarps,
1～2 part of microbial fermentation microbial inoculum,
24～26 parts of crop straw ashes,
4.5～5.5 parts of vinelandii microbial inoculums,
310～320 parts of vermiculites.
The preparation method of the special-purpose culture substrate of leaf class organic vegetable of the present invention, step is following:
(1) getting furfural dregs, the dregs of fat, scarp and microbial fermentation microbial inoculum by proportioning mixes.The moisture of adjustment compound is 55 ~ 60wt%, and fermentation reactor system becomes thoroughly decomposed, and temperature rises to 55 ℃ of timing, keeps 3 ~ 5 days.Turning, temperature rise to 50 ℃ of timing once more, keep 3 ~ 5 days.So operation repeats 2 ~ 3 times again, ferments altogether 18～40 days.Material must ferment.The material that should ferment carries out steam sterilizing, feeds steam, and temperature rises to 100 ℃ of timing, keeps 6～8 hours, gets the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum:
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, agar 18 grams, water 1000 grams are regulated pH to 7.0～7.5.Sterilization made the test tube slant in 30 minutes under 0.11 MPa pressure.Inoculation vinelandii bacterial classification is placed in 28 ℃ of constant incubators and cultivated 5～7 days under aseptic condition.Get vinelandii inclined-plane seed.
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, water 1000 grams are regulated pH to 7.0～7.5, make liquid nutrient medium.1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks; By the prior art sterilization; Insert three in vinelandii inclined-plane seed in each triangular flask of cooling back, 3～5 millimeters of every diameters were cultivated 4 days under 28 ℃～30 ℃, 150 rev/mins conditions.Get the vinelandii liquid bacterial agent.Bacterium liquid contains viable count 4 * 10
8Individual/gram-7 * 10
8Individual/gram.
(3) by proportioning; Get in the sterilization fermentation material that crop straw ash adds step (1) preparation, the pH that regulates this fermentation material is 7.0～7.5, adds 2～6 parts of the vinelandii liquid bacterial agents that step (2) prepares again; Mix; The moisture of adjustment compound is 55 ~ 60wt%, and composting is 3～5 days under 28 ℃～30 ℃ conditions, must contain the mixture of vinelandii.
(4) by proportioning, vermiculite is added in the mixture that contains vinelandii of step (3) preparation, mix, dry in the air to moisture content 27～30wt%, get culture substrate of the present invention.
Preferably, the vinelandii bacterial classification is selected from blown-ball Azotobacter ACCC01077 or ACCC11104 in the step (2).
The application method of the special-purpose culture substrate of leaf class organic vegetable of the present invention:
With this culture substrate cultivating container (basin or geosyncline etc.) of packing into, thickness is 13～20 centimetres, and by the ordinary method sowing vegetable seed or the seedling of planting, control measures are pressed the common cultivation matrix technology.
Culture substrate of the present invention contains a large amount of active vinelandii, and they can continue breeding in matrix in the vegetable growing process, and then the nitrogen in the fixed air continuously; For vegetables provide more nitrogen nutrition; And promoted to increase vegetable crop by nutrient balance, improve quality.The present invention has obtained good effect through overtesting.Excellent results of the present invention is following:
1. nitrogen content in the increase matrix
The special-purpose culture substrate of leaf class organic vegetable of the present invention is main with organic substance; Be the good cultivation materials of vinelandii; Conditions such as its moisture, temperature, ventilation also are fit to the vinelandii breeding very much in the vegetable growth process; Therefore nitrogen in the fixed air constantly increases the nitrogen content in the matrix.
2. promote culture substrate nutrient supply balance
The more nitrogen nutrition of good growth needs of leafy vegetable, nitrogen content is not enough in the common cultivation matrix, and mainly nutrition can not the balance supply to make nitrogen, phosphorus, potassium etc.Constantly the vinelandii of breeding can be under normal temperature and pressure conditions in the matrix, and the effect through nitrogenase in its body is fixed up airborne nitrogen, forms the nitrogen that plant can absorb, and promotes nutrient supply balance in the matrix.
3. raising vegetable crop
Nutrient supply abundance and balance are the bases of good growth of leafy vegetable and high yield, and comparing leafy vegetable with general crop needs more nitrogen nutrition, and common cultivation matrix can not meet the demands.The present invention has increased nitrogen content in the culture substrate through adding vinelandii in matrix, has promoted the balance between the nutrient, has improved leafy vegetable output.
Embodiment
Below in conjunction with embodiment the present invention is further specified, but be not limited thereto.Ratio, content are mass ratio among the embodiment.Raw material is explained as follows:
The vinelandii bacterial classification is: blown-ball Azotobacter ACCC01077 or ACCC11104, and Chinese agriculture microbial strains preservation administrative center buys, and wherein, embodiment 1-2 uses ACCC01077, and embodiment 3-4 uses ACCC11104.
Furfural dregs, Henan Hongye Chemical Industry Co., Ltd is that main raw material is made the waste residue in the furfural process with the corn cob.
Scarp, depleted tobacco leaf waste residue in China Tobacco Henan Industrial Corporation's production process.
The dregs of fat are remaining residue behind the Shandong wide life limited-liability company production edible oil, are the combinations with 1: 1 mass ratio of the soya-bean oil dregs of fat and the peanut oil dregs of fat.
The microbial fermentation microbial inoculum is the EM composite fungus agent, and Jiangxi Tianyi Biologic Technology Development Co., Ltd. produces.
The crop straw ash is a corn stalk ash, and Shandong Province's Heze City is with sharp biotechnology ltd sub product.
Vermiculite, the permanent vermiculite in source, Lingshou County, Hebei province source mill, particle diameter 2-4mm.
Embodiment 1:
The special-purpose culture substrate of leaf class organic vegetable, material component is following:
Furfural dregs 400kg,
Dregs of fat 50kg,
Scarp 210kg,
Microbial fermentation microbial inoculum 3kg,
Crop straw ash 35kg,
Vinelandii microbial inoculum 2kg,
Vermiculite 300kg.
The preparation method:
(1) get 400 kilograms of furfural dregs, 50 kilograms of the dregs of fat, 210 kilograms of scarps, 3 kilograms of microbial fermentation microbial inoculums mix.The moisture of adjustment compound is 55wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 4 days.Turning, temperature rise to 50 ℃ of timing once more, keep 3 days.So operation repeats 3 times again, ferments altogether 28 days.Material must ferment.The material that should ferment carries out steam sterilizing, feeds steam, and temperature rises to 100 ℃ of timing, keeps 6 hours, gets the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum:
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, agar 18 grams, water 1000 grams are regulated pH7.3.Sterilization made the test tube slant in 30 minutes under 0.11 MPa pressure.The inclined-plane has made back inoculation vinelandii bacterial classification ACCC01077 under aseptic condition, is placed in 28 ℃ of constant incubators and cultivates 6 days.Get vinelandii inclined-plane seed.
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 restrains, sodium-chlor 0.1 gram, lime carbonate 5 grams, water 1000 grams are regulated pH7.2, get liquid nutrient medium.1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks, by the prior art sterilization, insert three in vinelandii inclined-plane seed in each triangular flask of cooling back, 3 millimeters of every diameters were cultivated 4 days under 28 ℃, 150 rev/mins conditions.Get the vinelandii liquid bacterial agent.Bacterium liquid contains viable count 4.38 * 10
8Individual/gram.
(3) get in the sterilization fermentation material of crop straw ash 35 kilograms of addings step (1) preparation; The pH that regulates this fermentation material is 7.4; Add 2 kilograms of the vinelandii liquid bacterial agents of step (2) preparation again, mix, the moisture of adjustment compound is 60wt%; Composting is 4 days under 28 ℃ of conditions, must contain the mixture of vinelandii.
(4) get 300 kilograms of vermiculites, add in the mixture that contains vinelandii of step (3) preparation, mix, dry in the air, get culture substrate of the present invention to moisture content 28%.
Embodiment 2:
The special-purpose culture substrate of leaf class organic vegetable, material component is following:
Furfural dregs 300kg,
Dregs of fat 70kg,
Scarp 255kg,
Microbial fermentation microbial inoculum 2kg,
Crop straw ash 20kg,
Vinelandii microbial inoculum 3kg,
Vermiculite 350kg.
The preparation method:
(1) get 300 kilograms of furfural dregs, 70 kilograms of the dregs of fat, 255 kilograms of scarps, 2 kilograms of microbial fermentation microbial inoculums mix.The moisture of adjustment compound is 60wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 5 days.Turning, temperature rise to 50 ℃ of timing once more, keep 3 days.So operation repeats 2 times again, ferments altogether 24 days.Material must ferment.The material that should ferment carries out steam sterilizing, feeds steam, and temperature rises to 100 ℃ of timing, keeps 8 hours, gets the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum is with embodiment 1.
(3) get in the sterilization fermentation material of crop straw ash 20 kilograms of addings step (1) preparation; The pH that regulates this fermentation material is 7.1; Add 3 kilograms of the vinelandii liquid bacterial agents of step (2) preparation again, mix, the moisture of adjustment compound is 55wt%; Composting is 3 days under 30 ℃ of conditions, must contain the mixture of vinelandii.
(4) get 350 kilograms of vermiculites, add in the mixture that contains vinelandii of step (3) preparation, mix, dry in the air, get culture substrate of the present invention to moisture content 30%.
Embodiment 3:
The special-purpose culture substrate of leaf class organic vegetable, material component is following:
Furfural dregs 350kg,
Dregs of fat 100kg,
Scarp 200kg,
Microbial fermentation microbial inoculum 1kg,
Crop straw ash 25kg,
Vinelandii microbial inoculum 5kg,
Vermiculite 319kg.
The preparation method:
(1) get 350 kilograms of furfural dregs, 100 kilograms of the dregs of fat, 200 kilograms of scarps, 1 kilogram of microbial fermentation microbial inoculum mixes.The moisture of adjustment compound is 58wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 4 days.Turning, temperature rise to 50 ℃ of timing once more, keep 4 days.So operation repeats 2 times again, ferments altogether 24 days.Material must ferment.The material that should ferment carries out steam sterilizing, feeds steam, and temperature rises to 100 ℃ of timing, keeps 7 hours, gets the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum is with embodiment 1, and different is that the vinelandii bacterial classification adopts blown-ball Azotobacter ACCC11104, and the effective bacterial content of bacterium liquid is 5.96 * 10
8Individual/gram.
(3) get in the sterilization fermentation material of crop straw ash 25 kilograms of addings step (1) preparation; The pH that regulates this fermentation material is 7.2; Add 5 kilograms of the vinelandii liquid bacterial agents of step (2) preparation again, mix, the moisture of adjustment compound is 55wt%; Composting is 4 days under 30 ℃ of conditions, must contain the mixture of vinelandii.
(4) get 319 kilograms of vermiculites, add in the mixture that contains vinelandii of step (3) preparation, mix, dry in the air, get culture substrate of the present invention to moisture content 29%.
Embodiment 4:
The special-purpose culture substrate of leaf class organic vegetable, material component is following:
Furfural dregs 250kg,
Dregs of fat 120kg,
Scarp 300kg,
Microbial fermentation microbial inoculum 2kg,
Crop straw ash 40kg,
Vinelandii microbial inoculum 4kg,
Vermiculite 284kg.
The preparation method:
(1) get 250 kilograms of furfural dregs, dregs of fat 120 kg, 300 kilograms of scarps, 2 kilograms of microbial fermentation microbial inoculums mix.The moisture of adjustment compound is 58wt%, and fermentation reactor system becomes thoroughly decomposed.Temperature rises to 55 ℃ of timing, keeps 5 days.Turning, temperature rise to 50 ℃ of timing once more, keep 5 days.So operation repeats 3 times again, ferments altogether 40 days.Material must ferment.The material that should ferment carries out steam sterilizing, feeds steam, and temperature rises to 100 ℃ of timing, keeps 7 hours, gets the sterilization fermentation material.
(2) preparation of vinelandii microbial inoculum is with embodiment 1, and different is that the vinelandii bacterial classification adopts blown-ball Azotobacter ACCC11104, and the effective bacterial content of bacterium liquid is 5.96 * 10
8Individual/gram.
(3) get in the sterilization fermentation material of crop straw ash 40 kilograms of addings step (1) preparation; The pH that regulates this fermentation material is 7.5; Add 4 kilograms of the vinelandii liquid bacterial agents of step (2) preparation again, mix, the moisture of adjustment compound is 58wt%; Composting is 5 days under 28 ℃ of conditions, must contain the mixture of vinelandii.
(4) get 284 kilograms of vermiculites, add in the mixture that contains vinelandii of step (3) preparation, mix, dry in the air, get culture substrate of the present invention to moisture content 27%.
Comparative Examples: the coventional type culture substrate, Shandong Guangda Fertilizer Technology Co., Ltd. produces, and mainly is that organism and the vermiculite that becomes thoroughly decomposed makes with 2: 1 mixed of mass ratio.Organic matter raw material is Xylitol slag and the furfural dregs compsn with 1: 1 mass ratio.
The effect of above embodiment 1～4 and Comparative Examples is following:
Test place: the county that conquers east, Shandong Province, Shandong Guangda Fertilizer Technology Co., Ltd. test farm.
Test period: year April in January, 2012 to 2012.
The cultivation of employing geosyncline, 20 centimetres of groove depths, wide 40 centimetres, long 800 centimetres, the degree of depth of culture substrate in geosyncline is 16 centimetres, plantation 3 row.
One, supplying to study thing is celery, and kind is that texts and pictures draw.Effect is seen table 1.
Table 1. is compared the effect of embodiment 1-4 with Comparative Examples
| Culture substrate of the present invention | Stimulation ratio % | Edible part crude protein improves % |
| Embodiment 1 | 8.3 | 0.12 |
| Embodiment 2 | 7.9 | 0.12 |
| Embodiment 3 | 10.2 | 0.08 |
| Embodiment 4 | 6.5 | 0.10 |
Two, supplying to study thing is romaine lettuce, and experimental cultivar is the glass romaine lettuce.Effect is seen table 2.
Table 2. is compared the effect of embodiment 1-4 culture substrate with Comparative Examples
| Culture substrate of the present invention | Stimulation ratio % | Edible part crude protein improves % |
| Embodiment 1 | 5.8 | 0.12 |
| Embodiment 2 | 6.6 | 0.11 |
| Embodiment 3 | 7.3 | 0.13 |
| Embodiment 4 | 6.9 | 0.13 |
Claims (4)
1. the special-purpose culture substrate of leaf class organic vegetable is characterized in that raw materials quality part is following:
250～400 parts of furfural dregs,
50～120 parts of the dregs of fat,
180～300 parts of scarps,
1～3 part of microbial fermentation microbial inoculum,
20～40 parts of crop straw ashes,
2～6 parts of vinelandii microbial inoculums,
280～350 parts of vermiculites;
Said furfural dregs is that corn cob is that main raw material is made the waste residue in the furfural process;
Described scarp is a depleted tobacco leaf residue in the production of cigarettes process;
Described microbial fermentation microbial inoculum is the EM composite fungus agent;
Described vinelandii microbial inoculum is the liquid microbe microbial inoculum that azotobacter prepares through steps such as cultivation, fermentations by prior art, and bacterium liquid contains viable count 4 * 10
8Individual/gram～7 * 10
8Individual/gram;
The vinelandii bacterial classification is selected from blown-ball Azotobacter (Azotobacter chrooccum), Wei Nielande vinelandii (Azotobacter vinelandii), vinelandii (Azotobacter sp.).
2. the special-purpose culture substrate of leaf class organic vegetable according to claim 1 is characterized in that raw materials quality part is following:
340～360 parts of furfural dregs,
95～105 parts of the dregs of fat,
190～210 parts of scarps,
1～2 part of microbial fermentation microbial inoculum,
24～26 parts of crop straw ashes,
4.5～5.5 parts of vinelandii microbial inoculums,
310～320 parts of vermiculites.
3. the special-purpose culture substrate of leaf class organic vegetable according to claim 1 is characterized in that the vinelandii bacterial classification is selected from blown-ball Azotobacter ACCC01077 or ACCC11104.
4. the preparation method of the special-purpose culture substrate of each described leaf class organic vegetable of claim 1 ~ 3, step is following:
(1) by proportioning, get furfural dregs, the dregs of fat, scarp, the microbial fermentation microbial inoculum mixes; The moisture of adjustment compound is 55 ~ 60wt%, and fermentation reactor system becomes thoroughly decomposed, and temperature rises to 55 ℃ of timing, keeps 3 ~ 5 days; Turning, temperature rise to 50 ℃ of timing once more, keep 3 ~ 5 days; So operation repeats 2 ~ 3 times again, ferments altogether 18～40 days; Material must ferment.The material that should ferment carries out steam sterilizing, feeds steam, and temperature rises to 100 ℃ of timing, keeps 6～8 hours, gets the sterilization fermentation material;
(2) preparation of vinelandii microbial inoculum
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, agar 18 grams, water 1000 grams are regulated pH to 7.0～7.5; Sterilization made the test tube slant in 30 minutes under 0.11 MPa pressure; Inoculation vinelandii bacterial classification is placed in 28 ℃ of constant incubators and cultivated 5～7 days under aseptic condition; Get vinelandii inclined-plane seed;
Get sucrose 10 grams, potassium primary phosphate 0.2 gram, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.2 gram, calcium sulfate 0.2 gram, sodium-chlor 0.1 gram, lime carbonate 5 grams, water 1000 grams are regulated pH to 7.0～7.5, make liquid nutrient medium; 1000 gram liquid nutrient medium average marks are contained in three 500 milliliters of triangular flasks; By the prior art sterilization; Insert three in vinelandii inclined-plane seed in each triangular flask of cooling back, 3～5 millimeters of every diameters were cultivated 4 days under 28 ℃～30 ℃, 150 rev/mins conditions; Get the vinelandii liquid bacterial agent, bacterium liquid contains viable count 4 * 10
8Individual/gram-7 * 10
8Individual/gram;
(3) by proportioning; Get the crop straw ash and add in the sterilization fermentation material of step (1) preparation, the pH that regulates this fermentation material is 7.0～7.5, adds the vinelandii liquid bacterial agent of step (2) preparation again; Mix; The adjustment mixture moisture is 55 ~ 60wt%, and composting is 3～5 days under 28 ℃～30 ℃ conditions, must contain the mixture of vinelandii;
(4) by proportioning, vermiculite is added in the mixture that contains vinelandii of step (3) preparation, mix, dry in the air to moisture content 27～30wt%, promptly get.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN104591900A (en) * | 2015-01-29 | 2015-05-06 | 孙丽君 | Potted vegetable nutrient soil and preparation method thereof |
| CN104973977A (en) * | 2015-07-09 | 2015-10-14 | 广西宏华生物实业股份有限公司 | Special matrix for organic vegetable soilless cultivation, and preparation method thereof |
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| CN102701874B (en) | 2013-12-18 |
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