CN102690834B - 表达系统 - Google Patents

表达系统 Download PDF

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Publication number
CN102690834B
CN102690834B CN201210056815.2A CN201210056815A CN102690834B CN 102690834 B CN102690834 B CN 102690834B CN 201210056815 A CN201210056815 A CN 201210056815A CN 102690834 B CN102690834 B CN 102690834B
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sequence
promoter
plasmid
operator
vector
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English (en)
Chinese (zh)
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CN102690834A (zh
Inventor
I·J·霍奇森
C·D·J·伦农
B·V·卡拉
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Fujifilm Diosynth Biotechnologies UK Ltd
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Avecia Biologics Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201210056815.2A 2006-02-03 2007-02-01 表达系统 Active CN102690834B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0602173.7A GB0602173D0 (en) 2006-02-03 2006-02-03 Expression system
GB0602173.7 2006-02-03
CN2007800112768A CN101410523B (zh) 2006-02-03 2007-02-01 表达系统

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2007800112768A Division CN101410523B (zh) 2006-02-03 2007-02-01 表达系统

Publications (2)

Publication Number Publication Date
CN102690834A CN102690834A (zh) 2012-09-26
CN102690834B true CN102690834B (zh) 2018-09-04

Family

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CN201210056815.2A Active CN102690834B (zh) 2006-02-03 2007-02-01 表达系统
CN2007800112768A Active CN101410523B (zh) 2006-02-03 2007-02-01 表达系统
CN201810774783.7A Active CN108841848B (zh) 2006-02-03 2007-02-01 表达系统

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CN2007800112768A Active CN101410523B (zh) 2006-02-03 2007-02-01 表达系统
CN201810774783.7A Active CN108841848B (zh) 2006-02-03 2007-02-01 表达系统

Country Status (15)

Country Link
US (4) US8530188B2 (https=)
EP (6) EP1984506B1 (https=)
JP (5) JP5221385B2 (https=)
KR (3) KR101624345B1 (https=)
CN (3) CN102690834B (https=)
CA (2) CA2959915C (https=)
DK (4) DK2695943T3 (https=)
ES (3) ES2569075T3 (https=)
GB (1) GB0602173D0 (https=)
HU (1) HUE027350T2 (https=)
PL (3) PL2695943T3 (https=)
PT (2) PT1984506E (https=)
SG (3) SG169390A1 (https=)
WO (1) WO2007088371A2 (https=)
ZA (2) ZA200806238B (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
GB0709061D0 (en) * 2007-05-11 2007-06-20 Avecia Biolog Ltd Expression system
GB0810154D0 (en) 2008-06-04 2008-07-09 Avecia Biolog Ltd Expression vector
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process
GB201018664D0 (en) 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
GB201209896D0 (en) 2012-06-01 2012-07-18 Fujifilm Diosynth Biotechnologies Uk Ltd Process
TW201514193A (zh) 2013-01-31 2015-04-16 Glaxo Group Ltd 製備蛋白質之方法
US9873891B2 (en) * 2013-05-03 2018-01-23 Fujifilm Diosynth Biotechnologies Uk Limited Expression process
GB201311837D0 (en) 2013-07-01 2013-08-14 Fujifilm Diosynth Biotechnologies Uk Ltd Expression Process
GB201319930D0 (en) 2013-11-12 2013-12-25 Fujifilm Diosynth Biotechnologies Uk Ltd Expression process
JP6803328B2 (ja) * 2014-08-28 2021-01-06 シンセティック バイオロジクス，インコーポレイテッド 大腸菌ベースでのベータラクタマーゼ生産
US9791697B2 (en) 2015-12-11 2017-10-17 Panasonic Intellectual Property Management Co., Ltd. Screen
EP3436610A1 (en) * 2016-03-29 2019-02-06 Geltor, Inc. Expression of proteins in gram-negative bacteria wherein the ratio of periplasmic volume to cytoplasmic volume is between 0.5:1 and 10:1
BR112018072043A2 (pt) 2016-05-06 2019-02-12 Glaxosmithkline Intellectual Property Development Limited sequências de sinal e de ácido nucleico recombinantes, proteína de fusão recombinante, vetor de expressão recombinante, e, métodos para produzir uma proteína recombinante e controlar a viscosidade de um caldo de célula hospedeira e a expressão de uma proteína recombinante
GB201704659D0 (en) * 2017-03-24 2017-05-10 Fujifilm Diosynth Biotechnologies Uk Ltd Expression system
US20230151071A1 (en) * 2020-04-09 2023-05-18 United States Government As Represented By The Department Of Veterans Affairs Compositions comprising recombinant epo and methods of use thereof
US20230167467A1 (en) * 2020-04-15 2023-06-01 University of Alaska Anchorage Methods and compositions for the production of isobutene
CN118488963A (zh) 2021-12-17 2024-08-13 肯特大学 囊泡中生产重组生物分子的序列和方法

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Promoters largely determine the efficiency of repressor action;MICHAEL LANZER et al.;《Proc.Natl.Acad.Sci.USA》;19881231;第85卷;第8974页图1 *
Promoters largely determine the efficiency of repressor action;MICHAEL LANZER et al.;《Proc.Natl.Acad.Sci.USA》;19881231;第85卷;第8974页左栏第1段，图1 *
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WO2007088371A3 (en) 2007-10-04
PT2386642E (pt) 2014-02-12
JP2017136072A (ja) 2017-08-10
JP6571707B2 (ja) 2019-09-04
JP2009525038A (ja) 2009-07-09
EP2386644B1 (en) 2015-12-16
KR101624345B1 (ko) 2016-06-07
EP2386642B1 (en) 2013-11-13
DK2695943T3 (en) 2016-04-18
EP2695943B1 (en) 2016-01-20
US11098335B2 (en) 2021-08-24
CN101410523A (zh) 2009-04-15
EP2386642A2 (en) 2011-11-16
EP2390334A2 (en) 2011-11-30
CN108841848B (zh) 2022-03-29
KR101512776B1 (ko) 2015-04-16
US20090170160A1 (en) 2009-07-02
SG169389A1 (en) 2011-03-30
JP5221385B2 (ja) 2013-06-26
EP2695943A1 (en) 2014-02-12
GB0602173D0 (en) 2006-03-15
DK2386644T3 (en) 2016-03-14
JP5713984B2 (ja) 2015-05-07
HUE027350T2 (hu) 2016-09-28
EP2386643A3 (en) 2012-11-28
EP2390334A3 (en) 2012-11-28
EP2386643A2 (en) 2011-11-16
JP2016136959A (ja) 2016-08-04
EP2386642A3 (en) 2012-06-13
SG169390A1 (en) 2011-03-30
PT1984506E (pt) 2013-09-06
CA2959915A1 (en) 2007-08-09
SG169388A1 (en) 2011-03-30
US20210348205A1 (en) 2021-11-11
PL2695943T3 (pl) 2016-07-29
EP2390334B1 (en) 2016-08-10
ZA201004904B (en) 2011-03-30
US20170240943A1 (en) 2017-08-24
ES2445173T3 (es) 2014-02-28
EP1984506A2 (en) 2008-10-29
CN108841848A (zh) 2018-11-20
KR20140119202A (ko) 2014-10-08
JP2013078323A (ja) 2013-05-02
EP2386644A3 (en) 2012-11-28
ES2436883T3 (es) 2014-01-07
PL1984506T3 (pl) 2013-11-29
JP6298481B2 (ja) 2018-03-20
CA2959915C (en) 2022-11-15
KR20080098013A (ko) 2008-11-06
EP2386644A2 (en) 2011-11-16
EP2386643B1 (en) 2017-04-12
CN102690834A (zh) 2012-09-26
DK2386642T3 (en) 2014-02-17
JP2015006193A (ja) 2015-01-15
US9677103B2 (en) 2017-06-13
KR101492844B1 (ko) 2015-02-16
WO2007088371A2 (en) 2007-08-09
ZA200806238B (en) 2010-10-27
CA2637818A1 (en) 2007-08-09
CN101410523B (zh) 2012-05-16
CA2637818C (en) 2017-05-02
US20140065670A1 (en) 2014-03-06
KR20140090699A (ko) 2014-07-17
PL2386642T3 (pl) 2014-04-30
ES2569075T3 (es) 2016-05-06
US8530188B2 (en) 2013-09-10
EP1984506B1 (en) 2013-06-05
DK1984506T3 (da) 2013-09-16

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