CN102687804B - Recombinant lactobacillus and Chinese herbal biostime, biostime preparation and uses thereof - Google Patents
Recombinant lactobacillus and Chinese herbal biostime, biostime preparation and uses thereof Download PDFInfo
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Abstract
The invention relates to the technical field of microbial additives, and specifically discloses a recombinant lactobacillus and a Chinese herbal biostime and further discloses a biostime preparation prepared from the biostime and application thereof. The recombinant lactobacillus and the Chinese herbal biostime are composed of live recombinant lactobacillus powder and a Chinese herbal medicine, and the weight ratio of the live recombinant lactobacillus powder to the Chinese herbal medicine is 1: 50; the number of live bacteria in the live recombinant lactobacillus powder is (5 to 8)*10<11> per gram, and the Chinese herbal medicine is composed of the following crude drugs in parts by weight: 4 parts of astragalus root, 4 parts of root of pilose asiabell, 4 parts of medicated leaven, 4 parts of malt, 4 parts of pachyma cocos and 1 part of liquorice. The recombinant lactobacillus and the Chinese herbal biostime provided by the invention can serve as additives used in feed and are significant for increasing growth speed, reducing feed-gain rate and raising economical benefit of livestock and poultry, especially young animals.
Description
Technical field
The present invention relates to microbe additive technical field, be specifically related to a kind of recombinant lactobacilli and Chinese herbal medicine symphysis unit, also relate to symphysis unit preparation of being made by this symphysis unit and uses thereof simultaneously.
Background technology
Along with antibiotic, hormone etc. are as the abuse of feed addictive, its harmfulness is familiar with by people gradually, therefore, forbid that adding these chemicalses with additive form has become undisputable fact, moreover, in developed country, as the U.S., European Union member countries and some other country carry out.But go back this phenomenon of ubiquity in the feedstuff industry of China, main or poor because of the breeding environment of China's entirety, these medicines have been left in animal feeding, their health and growth all can be affected so.So want to promote the healthy aquaculture of China animal, improve the quality of animal product, just must find a kind of can substitute antibiotics or the product of hormone, this product not only can promote growth and the health of animal as feed addictive, the more important thing is can not be residual in vivo.
Summary of the invention
The object of the present invention is to provide a kind of recombinant lactobacilli and Chinese herbal medicine symphysis unit.
The present invention also aims to provide a kind of symphysis unit preparation of being made by recombinant lactobacilli and Chinese herbal medicine symphysis unit.
The present invention also aims to provide the purposes of a kind of recombinant lactobacilli and Chinese herbal medicine symphysis unit.
The present invention also aims to provide a kind of purposes of the symphysis unit preparation of being made by recombinant lactobacilli and Chinese herbal medicine symphysis unit.
In order to realize above object, the technical solution adopted in the present invention is: a kind of recombinant lactobacilli and Chinese herbal medicine symphysis unit, be made up of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine, the weight proportion of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine is: recombinant lactobacilli viable bacteria powder: Chinese herbal medicine=1: 50; In recombinant lactobacilli viable bacteria powder, viable count is (5～8) × 10
11individual/gram, described Chinese herbal medicine is made up of the bulk drug of following parts by weight: 4 parts of the Radixs Astragali, 4 parts of Radix Codonopsis, 4 parts of Divine Comedy, 4 parts, Fructus Hordei Germinatus, 4 parts, Poria cocos, 1 part, Radix Glycyrrhizae.
A symphysis unit preparation of being made up of described recombinant lactobacilli and Chinese herbal medicine symphysis unit, by recombinant lactobacilli and Chinese herbal medicine symphysis unit, CaCO
3, adhesive, suspending agent, disintegrant composition, wherein the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 88～92%, CaCO
3weight percentage be 1%, the weight percentage of adhesive is 1～5%, the weight percentage of suspending agent is 0.1～1%, the weight percentage of disintegrant is 1～5%.
The first preparation of described symphysis is pulvis, tablet or liquid preparation.
Described adhesive is starch, and described suspending agent is sodium alginate, and described disintegrant is sodium carboxymethyl starch.
A kind of recombinant lactobacilli and Chinese herbal medicine symphysis unit are as the application of chicken feed addictive.
When recombinant lactobacilli and Chinese herbal medicine symphysis unit apply as chicken feed addictive, the addition of recombinant lactobacilli and Chinese herbal medicine symphysis unit is: the weight of the Chinese herbal medicine in recombinant lactobacilli and Chinese herbal medicine symphysis unit is 0.1～0.4% of chicken feed weight.Preferably, the addition of recombinant lactobacilli and Chinese herbal medicine symphysis unit is: the weight of the Chinese herbal medicine in recombinant lactobacilli and Chinese herbal medicine symphysis unit is 0.3% of chicken feed weight.
A kind of symphysis unit preparation of being made up of recombinant lactobacilli and Chinese herbal medicine symphysis unit is as the application of chicken feed addictive.
When symphysis unit preparation is applied as chicken feed addictive, the addition of symphysis unit preparation is: the weight of the Chinese herbal medicine in symphysis unit preparation is 0.1～0.4% of chicken feed weight.Preferably, the addition of symphysis unit preparation is: the weight of the Chinese herbal medicine in symphysis unit preparation is 0.3% of chicken feed weight.
Recombinant lactobacilli wherein derives from Lactobacillus casei (Lactobacillus casei), and (deposit number is: ATCC No.5276, depositary institution: American Type Culture Collecti), by integrated amylase gene in Lactobacillus casei genome, and can high efficient expression.
What probiotics referred generally to is all the probio living, these probios can play adjusting colony balance at the enteron aisle of animal, suppress harmful bacteria, especially some functional probios, as the probio that secretes various digestive ferments, antibacterial peptide, hydrogen peroxide can produce multiple effect at enteron aisle.In animal feed, the microorganism of extensive use has Bacillus acidi lactici, Bifidobacterium, bacillus, saccharomycete etc. at present, facts have proved that these probiotics preparations have certain effect at aspect tools such as promoting growth of animal, prevention or treatment diarrhoea.
China's Chinese herbal medicine is of a great variety, and resource is very abundant, and the Chinese herbal medicine of finding has at present reached kind more than 12000.The multiple nutrients materials such as protein that Chinese herbal medicine contains, amino acid, vitamin, have growth promotion and disease-resistant effect to animal.Find that there is after deliberation more than 200 kind of Chinese herbal medicine and contain immunologic active material, can strengthen the immunologic function of animal body; There is more than 400 kind of Chinese herbal medicine to have sterilization, bacteriostasis; More than 50 kind of Chinese herbal medicine has deactivation or inhibitory action to virus.More especially the class of strengthening the body resistance to consolidate the constitution Chinese herbal medicine, as the Radix Astragali, Radix Codonopsis etc., except can enhanced machine body immunity function, also can promote the growth of probio.Because Chinese herbal medicine is natural materials, keep naturality and the biologically active of various compositions, easily absorbed by animal body and utilize, non-absorbently also can excrete smoothly, therefore, there is not the problem of medicament residue, so it is a kind of very desirable feed addictive.
Symphysis unit is a kind of by probio with can specificity promote the material (prebiotics) of proliferation of probiotics according to the preparation of certain proportioning use in conjunction, and it has the characteristic of probio and prebiotics simultaneously.Not only can be by the external probio regulating intestinal canal flora of feeding, but also can promote by prebiotics the propagation of intrinsic probio in intestines.Therefore, two kinds of components in symphysis unit can be mutually collaborative in enteron aisle, for enteron aisle is set up a good micro-ecological environment.
Diastatic recombinant lactobacilli is expressed as probio using the strain building by recombinant lactobacilli provided by the invention and Chinese herbal medicine symphysis unit, filter out the Chinese herbal medicine that can specificity promote this recombinant lactobacilli propagation by vitro test again, both are made after according to certain ratio proportioning.Show through in vitro test, the Chinese herbal medicine in recombinant lactobacilli provided by the invention and Chinese herbal medicine symphysis unit can promote the propagation of recombinant lactobacilli, suppresses the growth of Escherichia coli, salmonella; In vivo studies shows, symphysis unit can promote the growth of broiler chicken, improves immunity, reduces the death rate.Recombinant lactobacilli provided by the invention and Chinese herbal medicine symphysis unit can be used as additive application in feed, and for livestock and poultry, especially young animal, has great importance to improving the speed of growth, reduction feedstuff-meat ratio, increase economic benefit etc.
In recombinant lactobacilli provided by the invention and the first preparation of Chinese herbal medicine symphysis symphysis first and that made by it, recombinant lactobacilli is the microorganism formulation of living, after oral this active bacteria formulation, can field planting in enteron aisle, and in growth metabolism, produce many kinds of substance, as lactic acid, antibacterial peptide, digestive ferment, the amylase of especially recombinating in this bacterium, can be at the high efficient expression of enteron aisle, supplement the amylase in enteron aisle, in addition the multiple action of lactobacillus and Chinese herbal medicine, can suppress the growth of harmful bacteria, improve the production performance of animal, improve the immunity of body, reduce diarrhoea, build up health.Recombinant lactobacilli provided by the invention and Chinese herbal medicine symphysis unit and symphysis unit's preparation production for animal as feed addictive of being made up of it, for animal intestinal has been set up a good micro-ecological environment.
Recombinant lactobacilli provided by the invention symphysis unit preparation first with Chinese herbal medicine symphysis and that be made up of it has following advantage and effect compared with existing feed addictive:
1, security is good, noresidue
Symphysis unit provided by the invention and symphysis unit preparation be by recombinant lactobacilli and the Chinese herbal medicine that there is stomach strengthening and digestion promoting and strengthen immunity according to a certain percentage proportioning form, each composition is all containing poisonous and harmful substance, also can in body, not produce after therefore oral residual.
2, can supplement the amylase in enteron aisle
The component recombination lactobacillus of symphysis unit provided by the invention and symphysis unit preparation is that one is incorporated into the genetic engineering bacterium in Lactobacillus casei Lactobacillus cacei (ATCC5276) by technique for gene engineering by amylase gene, the feature of this recombination lactic acid bacillus is to contain expression plasmid pIlac-sp-amy, recombinant expression plasmid pIlac-sp-amy contains Bacillus acidi lactici replicon ori, ribosome bind site RBS, operon lac, Bacillus acidi lactici signal peptide gene (signal peptide, sp), amylase gene (amylase, amy), ribosome bind site RBS and resistance screening gene Erythromycin E ry (erythromycin, Ery) gene, its expression formula is :-ori-RBS-lac-sp-amy-RBS-Ery-, proving in vitro can efficient secretory expression amylase.
3, can be simultaneously by the lactobacillus in external source and endogenous increase enteron aisle
The prebiotics Chinese herbal medicine that contains recombinant lactobacilli and lactobacillus in the component of symphysis unit provided by the invention and symphysis unit preparation, therefore recombinant lactobacilli can directly increase the lactobacillus in enteron aisle, prebiotics Chinese herbal medicine not only can promote the propagation of recombinant lactobacilli, the propagation of the endogenic lactobacillus of enteron aisle can also be stimulated, the dominant microflora of probio can be in enteron aisle, set up as early as possible.
The specific embodiment
Embodiment 1
The recombinant lactobacilli that the present embodiment provides and Chinese herbal medicine symphysis unit, be made up of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine, and the weight proportion of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine is: recombinant lactobacilli viable bacteria powder: Chinese herbal medicine=1: 50; In recombinant lactobacilli viable bacteria powder, viable count is 5 × 10
11individual/gram, Chinese herbal medicine is made up of the bulk drug of following parts by weight: 4 parts of the Radixs Astragali, 4 parts of Radix Codonopsis, 4 parts of Divine Comedy, 4 parts, Fructus Hordei Germinatus, 4 parts, Poria cocos, 1 part, Radix Glycyrrhizae.
Embodiment 2
The recombinant lactobacilli that the present embodiment provides and Chinese herbal medicine symphysis unit, be made up of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine, and the weight proportion of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine is: recombinant lactobacilli viable bacteria powder: Chinese herbal medicine=1: 50; In recombinant lactobacilli viable bacteria powder, viable count is 8 × 10
11individual/gram, Chinese herbal medicine is made up of the bulk drug of following parts by weight: 4 parts of the Radixs Astragali, 4 parts of Radix Codonopsis, 4 parts of Divine Comedy, 4 parts, Fructus Hordei Germinatus, 4 parts, Poria cocos, 1 part, Radix Glycyrrhizae.
Embodiment 3
The symphysis unit preparation that the present embodiment provides, the recombinant lactobacilli being provided by the embodiment of the present invention 1 and Chinese herbal medicine symphysis unit make, and it consists of: the recombinant lactobacilli being provided by the embodiment of the present invention 1 and Chinese herbal medicine symphysis unit, CaCO
3, starch, sodium alginate, sodium carboxymethyl starch composition, the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 88%, CaCO
3weight percentage be 1%, the weight percentage of starch is 5%, the weight percentage of sodium alginate is 1%, the weight percentage of sodium carboxymethyl starch is 5%.
The symphysis unit preparation that the present embodiment provides is pulvis, and its preparation method, with the preparation method of conventional pulvis, mixes rear packing by various raw materials and obtains pulvis.Every restrain symphysis unit preparation in containing 4,000,000,000 of recombinant lactobacillis.
Embodiment 4
The symphysis unit preparation that the present embodiment provides, the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit make, and it consists of: the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit, CaCO
3, starch, sodium alginate, sodium carboxymethyl starch composition, the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 92%, CaCO
3weight percentage be 1%, the weight percentage of starch is 3%, the weight percentage of sodium alginate is 0.5%, the weight percentage of sodium carboxymethyl starch is 3.5%.
The symphysis unit preparation that the present embodiment provides is tablet, and its preparation method is with the preparation method of conventional tablet, and after mixing by raw material, direct tablet compressing, obtains tablet after packing.Every restrain symphysis unit preparation in containing 3,600,000,000 of recombinant lactobacillis.
Embodiment 5
The symphysis unit preparation that the present embodiment provides, the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit make, and it consists of: the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit, CaCO
3, starch, sodium alginate, sodium carboxymethyl starch composition, the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 92%, CaCO
3weight percentage be 1%, the weight percentage of starch is 1%, the weight percentage of sodium alginate is 1%, the weight percentage of sodium carboxymethyl starch is 5%.
The symphysis unit preparation that the present embodiment provides is tablet, and its preparation method is with the preparation method of conventional tablet.
Embodiment 6
The symphysis unit preparation that the present embodiment provides, the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit make, and it consists of: the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit, CaCO
3, starch, sodium alginate, sodium carboxymethyl starch composition, the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 92%, CaCO
3weight percentage be 1%, the weight percentage of starch is 5%, the weight percentage of sodium alginate is 1%, the weight percentage of sodium carboxymethyl starch is 1%.
The symphysis unit preparation that the present embodiment provides is tablet, and its preparation method is with the preparation method of conventional tablet.
Embodiment 7
The symphysis unit preparation that the present embodiment provides, the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit make, and it consists of: the recombinant lactobacilli being provided by the embodiment of the present invention 2 and Chinese herbal medicine symphysis unit, CaCO
3, starch, sodium alginate, sodium carboxymethyl starch composition, the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 92%, CaCO
3weight percentage be 1%, the weight percentage of starch is 3%, the weight percentage of sodium alginate is 0.1%, the weight percentage of sodium carboxymethyl starch is 3.9%.
The symphysis unit preparation that the present embodiment provides is liquid preparation, and its preparation method is with the preparation method of conventional liq preparation.
Test example 1 proves that herbal prescription can promote as prebiotics the growth of recombinant lactobacilli
Bacterial classification: recombinant lactobacilli (coming from Lactobacillus casei Lactobacills casei ATCC5276);
Culture medium: peptone 10g, beef extract 10g, yeast extract 5g, K
2hPO
42g, sodium acetate 5g, dibasic ammonium citrate 2g, glucose 20g, Tween-80 1ml, MgSO
47H
2o 0.58g, MnSO
44H
2o0.25g, water 1000ml, adjust pH to 6.2;
The preparation of Chinese herbal medicine: press following parts by weight weighting raw materials: 4 parts of the Radixs Astragali, 4 parts of Radix Codonopsis, 4 parts of Divine Comedy, 4 parts, Fructus Hordei Germinatus, 4 parts, Poria cocos, 1 part, Radix Glycyrrhizae, by for subsequent use pulverizing medicinal materials powdered;
The impact of the extracts of Chinese herbal medicine of measuring variable concentrations on restructuring growth of lactobacillus: the recombinant lactobacilli liquid of activation is inoculated into respectively to contain percentage by weight be in 0.1%, 0.2%, 0.3%, 0.4% the culture medium of Chinese herbal medicine, take not containing the culture medium of Chinese herbal medicine as contrast, put 37 ℃ of constant temperature anaerobism shaken cultivation 24h, then drip kind of a concentration for method mensuration bacterium liquid with 10 times of dilution plates, measurement result is shown in Table 1.
Table 1 extracts of Chinese herbal medicine affects result of the test (viable count unit: 10 to restructuring growth of lactobacillus
11cFU/ML)
As can be seen from Table 1, the Chinese herbal medicine of variable concentrations all has growth promoting function to recombinant lactobacilli, but concentration growth-promoting effect the best 0.3% time.Along with increasing of concentration, can suppress growth.Draw as calculated, when the weight proportion of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine is: recombinant lactobacilli viable bacteria powder: Chinese herbal medicine=1: 50 time, Chinese herbal medicine growth-promoting effect the best to recombinant lactobacilli.
The impact test of the extracts of Chinese herbal medicine of test example 2 variable concentrations on Escherichia coli and Salmonella growth
Measure broth medium containing the extracts of Chinese herbal medicine growth effect to Escherichia coli and salmonella with the same method of test example 1, the results are shown in Table shown in 2.
Table 2 extracts of Chinese herbal medicine affects result of the test (viable count unit: 10 to restructuring growth of lactobacillus
9cFU/ML)
| 0.00％ | 0.1％ | 0.2％ | 0.3％ | 0.4％ | |
| Escherichia coli | 6.67 | 6.13 | 5.58 | 4.33 | 2.15 |
| Increase ratio | 0.92 | 0.84 | 0.65 | 0.32 | |
| Salmonella | 3.83 | 3.74 | 2.13 | 1.07 | 0.85 |
| Increase ratio | 0.98 | 0.56 | 0.27 | 0.22 |
As can be seen from Table 2, the Chinese herbal medicine of variable concentrations has the effect of inhibition growth in various degree to Escherichia coli and salmonella, and inhibition degree is relevant with the addition of Chinese herbal medicine, and concentration is higher, and inhibitory action is stronger.
The impact test of the symphysis unit preparation that test example 3 embodiment 3 provide on AA growth of meat chicken performance
1. material
1.1 animal used as test
Meat chick: purchased from Hebi meat kind chicken house, just gone out shell with 600 of batch AA meat chicks.
1.2 feed addictive
The symphysis unit preparation that embodiment 3 provides, the addition of symphysis unit preparation is: the weight of the Chinese herbal medicine in symphysis unit preparation is 0.3% of chicken feed weight.
1.3 experimental basis daily rations
Basal diet is with reference to the preparation of AA broiler chicken trophic level, and its composition and nutritional labeling are shown in Table 3.
Table 3 experimental basis daily ration composition and trophic level
Note: per kilogram premix is containing VA6000,000IU, VD33,300,000IU, VE27,000IU, VK31,500mg, VB120,000mg, VB26,000mg, VB64200mg, VB1210mg, nicotinic acid 4,500mg, pantothenic acid 4,000mg, folic acid 150mg, biotin 15mg, Choline Chloride 75g, Fe7,792mg, Cu 750mg, Zn 7,300mg, Mn 9,785mg, Se 33mg, I 150mg.
2. test method
2.1 experimental design and management
This test is selected and is just gone out shell with 600 of batch of goods AA meat chicks, and male and female half and half, is divided into 4 processing at random, 3 repetitions of each processing, 50 chickens of each repetition.Not remarkable (P > 0.05) of body weight difference between each processing, testing program is shown in Table 4, and the test period is 42 days.
Table 4 experimental design scheme
Note: 0.3% refers to the percentage by weight that accounts for basal diet.
Require to control temperature, humidity and light application time with reference to Beijing AA of Hua Dou broiler chicken company commercial meat bird feeding and management handbook.24h illumination in first 7 days, later 23h illumination, 1h dark, relative humidity 55～70%, the raising initial stage keeps 33 ℃ of left and right of room temperature, reduces gradually later, and minimum room temperature remains on 23 ℃ of left and right.Use weekly 0.1% bromogeramine band chicken spraying disinfection once, immune programme for children carries out routinely.Ground is flat supports, and adopts traditional water fountain freely drink water and search for food.
In feeding process, observe chicken health condition every day, record death toll, deduct in time feed consumption rate.
2.2 growth performances are measured
To repeat as unit, before test, weigh for every group, weigh (empty stomach 4h before weighing) in 7 ages in days, 14 ages in days, 21 ages in days, 28 ages in days, 35 ages in days, 42 ages in days, calculate Zhou Zengchong, daily gain, all feed consumption rates and daily material consumption, feedstuff-meat ratio, computing formula is as follows:
Gross weight/chicken number of the chicken of average Zhou Tichong=3 repetition;
Average Zhou Zengchong=next week all average weight of all average weights-upper a week;
Average daily gain=(opisthosoma weight-first body weight)/number of days;
Feedstuff-meat ratio=feed consumption rate/weightening finish;
Growth performance measurement result is in shown in Table 5-table 9.
The impact of table 5 different additive on AA broiler chicken 1-6 week average weight (unit: gram)
Note: the expression significant difference (P < 0.05) that same column lowercase is different, extremely significantly (the P < 0.01) of expression difference that capitalization is different, represent not remarkable (P > 0.05) of difference containing same letter, following table is same.
The impact of table 6 different additive on average Zhou Zengchong of AA broiler chicken 1-6 week (unit: gram)
The impact of table 7 different additive on each all daily gains of AA broiler chicken 1-6 week (unit: gram)
The impact of table 8 different additive on average feed consumption of AA broiler chicken 1-6 week (unit: gram)
The impact of table 9 different additive on AA broiler chicken 1-6 week material anharmonic ratio
Can be found out by table 5-table 9, recombinant lactobacilli is body weight, Zhou Zengchong and the daily gain aspect to age in different week of AA broiler chicken as additive, all can improve to a certain extent the production performance of AA broiler chicken, at the 2nd week, the 3rd week all significantly lower than control group (P < 0.05), other week age and age in days also all have reduction, but not remarkable (P > 0.05) of difference.
Can be found out by table 5-table 9, Chinese herbal medicine is body weight, Zhou Zengchong and the daily gain aspect to ages in different week of AA broiler chicken as additive, in the body weight of the 4th week (P < 0.01), 5 weeks (P < 0.05), 6 weeks (P < 0.01) compared with control group significant difference or extremely remarkable; And be significantly improved (P < 0.05) compared with control group Zhou Zengchong and the daily gain of the 4th week, but there is the utmost point to significantly improve (P < 0.01) compared with control group in average Zhou Zengchong and the daily gain in whole breeding cycle 0-6 week; Feed consumption also has increase in various degree, although material anharmonic ratio only at the 3rd week compared with extremely significantly (P < 0.01) of control group difference, the material anharmonic ratio of end eventually in 0-6 week significantly improves (P < 0.01) compared with the control group utmost point.
Can be found out by table 5-table 9, the impact of symphysis unit preparation body weight, Zhou Zengchong and daily gain aspect on age in different week of AA broiler chicken as feed addictive that embodiment 3 provides, aspect the average weight in each week, showing stronger weightening finish effect, just compared with control group significant difference (P < 0.05), and all there is all the other week age the utmost point to significantly improve (P < 0.01) at the 1st week; Zhou Zengchong and daily gain aspect started from the 2nd week compared with significantly (P < 0.05) or extremely significantly (P < 0.01) of control group weightening finish; Material anharmonic ratio is obvious especially, and except the 1st week, all the other feeding periods are all compared with extremely significantly (P < 0.01) of control group difference.
2.3 death rate
By the dead number of washing in a pan of repeating groups record, calculate the death rate of each processed group according to following formula:
Death rate=dead number/total chicken number (processed group) of washing in a pan.
Processed group AA broiler chicken death toll and survival rate the results are shown in Table shown in 10.
The impact of table 10 different additive on AA broiler chicken death toll and death rate
| Processed group | Raise number of elements | The 1st week | The 2nd week | The 3rd week | The 4th week | The 5th week | The 6th week | Amount to dead number of elements | Death rate (%) |
| I | 150 | 3 | 3 | 5 | 3 | 14 | 9.33 | ||
| II | 150 | 3 | 3 | 3 | 9 | 6 | |||
| III | 150 | 3 | 2 | 2 | 7 | 4.67 | |||
| IV | 150 | 1 | 1 | 2 | 1.33 |
As can be seen from Table 10, processed group IV i.e. the broiler chicken group of the edible feed that is added with the symphysis unit preparation that embodiment 3 provides, there is obvious effect at broiler chicken aspect disease-resistant, this group death rate is only 1.33%, survival rate reaches 98.67%, and recombinant lactobacilli group and Chinese herbal medicine group survival rate only have 94.0% and 95.33%, control group survival rate only has 90.67%.
Claims (10)
1. recombinant lactobacilli and a Chinese herbal medicine symphysis unit, is characterized in that, forms by expressing diastatic recombinant lactobacilli viable bacteria powder and Chinese herbal medicine, and the weight proportion of recombinant lactobacilli viable bacteria powder and Chinese herbal medicine is: recombinant lactobacilli viable bacteria powder: Chinese herbal medicine=1:50; In recombinant lactobacilli viable bacteria powder, viable count is (5～8) × 10
11individual/gram, described Chinese herbal medicine is made up of the bulk drug of following parts by weight: 4 parts of the Radixs Astragali, 4 parts of Radix Codonopsis, 4 parts of Divine Comedy, 4 parts, Fructus Hordei Germinatus, 4 parts, Poria cocos, 1 part, Radix Glycyrrhizae.
2. a symphysis unit preparation of being made up of recombinant lactobacilli described in claim 1 and Chinese herbal medicine symphysis unit, is characterized in that, by recombinant lactobacilli and Chinese herbal medicine symphysis unit, CaCO
3, adhesive, suspending agent, disintegrant composition, the weight percentage of recombinant lactobacilli and Chinese herbal medicine symphysis unit is 88～92%, CaCO
3weight percentage be 1%, the weight percentage of adhesive is 1～5%, the weight percentage of suspending agent is 0.1～1%, the weight percentage of disintegrant is 1～5%.
3. the first preparation of symphysis according to claim 2, is characterized in that, the first preparation of described symphysis is pulvis or tablet.
4. the first preparation of symphysis according to claim 2, is characterized in that, described adhesive is starch, and described suspending agent is sodium alginate, and described disintegrant is sodium carboxymethyl starch.
One kind as claimed in claim 1 recombinant lactobacilli and Chinese herbal medicine symphysis unit in the application of preparing in chicken feed addictive.
6. recombinant lactobacilli according to claim 5 and Chinese herbal medicine symphysis unit are in the application of preparing in chicken feed addictive, it is characterized in that, the addition of recombinant lactobacilli and Chinese herbal medicine symphysis unit is: the weight of the Chinese herbal medicine in recombinant lactobacilli and Chinese herbal medicine symphysis unit is 0.1～0.4% of chicken feed weight.
7. recombinant lactobacilli according to claim 6 and Chinese herbal medicine symphysis unit are in the application of preparing in chicken feed addictive, it is characterized in that, the addition of recombinant lactobacilli and Chinese herbal medicine symphysis unit is: the weight of the Chinese herbal medicine in recombinant lactobacilli and Chinese herbal medicine symphysis unit is 0.3% of chicken feed weight.
One kind as claimed in claim 2 symphysis unit preparation in the application of preparing in chicken feed addictive.
9. the first preparation of symphysis according to claim 8, in the application of preparing in chicken feed addictive, is characterized in that, the addition of symphysis unit preparation is: the weight of the Chinese herbal medicine in symphysis unit preparation is 0.1～0.4% of chicken feed weight.
10. the first preparation of symphysis according to claim 9, in the application of preparing in chicken feed addictive, is characterized in that, the addition of symphysis unit preparation is: the weight of the Chinese herbal medicine in symphysis unit preparation is 0.3% of chicken feed weight.
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| CN104206786A (en) * | 2013-09-30 | 2014-12-17 | 郑州后羿制药有限公司 | Traditional Chinese medicine microecological preparation for meat chicken, preparation method and applications thereof |
| CN104474530A (en) * | 2014-09-15 | 2015-04-01 | 江西核工业瑞丰生物药业有限公司 | Compound thousand-mesh superfine powder traditional Chinese veterinary preparation for promoting growth of animals and preparation method thereof |
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| CN101589769B (en) * | 2009-07-07 | 2012-07-04 | 金陵科技学院 | Dorking composite Chinese medicinal herb feed additive, preparation method thereof and use thereof |
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