CN102626049A - Cultivation method of black strawberries - Google Patents
Cultivation method of black strawberries Download PDFInfo
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- CN102626049A CN102626049A CN2012100955884A CN201210095588A CN102626049A CN 102626049 A CN102626049 A CN 102626049A CN 2012100955884 A CN2012100955884 A CN 2012100955884A CN 201210095588 A CN201210095588 A CN 201210095588A CN 102626049 A CN102626049 A CN 102626049A
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Abstract
The invention discloses a cultivation method of black strawberries. The method adopts black sesame pigment gene, culture media, potassium sulfate, ferric sulfate, ferrous sulfate, potassium peroxydisulfate, zinc sulfate, straws, corncobs and water as raw materials. The black sesame pigment gene which is extracted from black sesames is injected into embryos of strawberry seeds, and composite organic nutrient fluid containing trace elements such as potassium and iron are added into the soil to cultivate the black strawberries rich in vitamin E and ferrum. The strawberries is black, have large size and have uniform size; the vitamin E and ferrum contained by the black strawberries are easy to absorb by human bodies, the biotransformation efficiency is high, and a particular healing effect for patients with cerebral thrombosis can be achieved; germinating percentage of strawberry seedlings reaches up to 99.5 percent, the antiviral property is strong, the adversity resistance is strong, and the strawberries can be stored for 15 to 20 days under a low temperature of 5 to 10 DEG C.
Description
Technical field
The present invention relates to the cultivation method of a kind of black strawberry, belong to biological technical field.
Background technology
The Fragaria rose family, perennial herb, nutritious, have " fruit queen " good reputation, be the important source of the various nutrient components of necessary for human, the vitamin that is rich in is higher than other fruit far away, has effects such as the nourishing the liver of making eye bright, aid digestion, anti-cancer, and is all-ages.
Strawberry is the fruit of the unique outlet of China, and supply falls short of demand, and the relevant personnel are studying the culture technique of strawberry always both at home and abroad; Adopt the greenhouse cultivation strawberry, convenient management, production cost is low; Output is high, and income is good, but because of high temperature, how wet in the canopy; Being prone to influences the output of strawberry for damage by disease and insect takes place to create utmost point advantageous environment; Adopt plant tissue culture technique plantation strawberry, utilize strawberry to be organized in and carry out in-vitro propagate on the proper culture medium, can accelerate variety breeding speed, improve the productivity ratio of cultivating strawberry, keep its merit; Moreover the strawberry color is single, from long, antiviral, the degeneration-resistant border of planting to the cycle of going on the market, anti-storage property is poor.
Therefore; In order to enlarge strawberry market; Pursue low cost, high yield, high-quality, we are necessary to propose the culture technique of a kind of black strawberry, improve the unicity of strawberry color; Solve a series of problems of low, antiviral, the degeneration-resistant border of strawberry germination rate, anti-storage property difference, thereby reach raising the output, increase the purpose of matter.
Summary of the invention
The present invention is directed to the unicity of strawberry color, antiviral, degeneration-resistant border, anti-storage property are poor, and the cultivation method of a kind of black strawberry is provided; Through gene transfer technique, with the embryo who extracts the Semen sesami nigrum chromogene injection strawberry seed that obtains in the Semen sesami nigrum, and adding contains micro-compound organic nutritive liquid such as potassium, iron in soil; Make that the strawberry seedling germination rate of cultivating is high, antiviral property, degeneration-resistant border property enhancing, fruit black in color; Particle is big; Anti-storage, and contain abundant vitamin E, iron can have special curative effect to the brain blood patient that declines.
The raw material that the present invention adopted is: Semen sesami nigrum chromogene, medium, potassium sulphate, iron sulfate, sulfurous acid iron, potassium peroxydisulfate, zinc sulphate, stalk, corncob, water; Pass through gene transfer technique; With the embryo who extracts the Semen sesami nigrum chromogene injection strawberry seed that obtains in the Semen sesami nigrum, and adding contains micro-compound organic nutritive liquid such as potassium, iron in soil, the feasible strawberry seedling germination rate height of cultivating; Antiviral property, degeneration-resistant border property enhancing, output increases; The fruit black in color, particle is big, anti-storage; And contain abundant vitamin E, ferro element, be easy to absorption of human body, the biological transformation ratio height can have special curative effect to the brain blood patient that declines.
The technical scheme that the present invention adopts is:
The cultivation method of a kind of black strawberry; It is raw material that this method adopts Semen sesami nigrum chromogene, medium, potassium sulphate, iron sulfate, sulfurous acid iron, potassium peroxydisulfate, zinc sulphate, stalk, corncob, water; Pass through gene transfer technique; With the embryo who extracts the Semen sesami nigrum chromogene injection strawberry seed that obtains in the Semen sesami nigrum, and adding contains micro-compound organic nutritive liquid such as potassium, iron in soil, nurtures the strawberry of the black of a kind of rich vitamins E.
The cultivation process of black strawberry among the present invention:
(1) preparation of medium
By the quality of each composition, glucose 8 ~ 12g, dipotassium hydrogen phosphate 0.6 ~ 1.0g, magnesium sulfate 0.1 ~ 0.2g, agar 12 ~ 16g, nitrate of lime 0.2 ~ 0.5g, iron sulfate 0.4 ~ 0.8g; With each components dissolved, stirring, adding distil water is settled to 1000mL, packing, high temperature sterilization, subsequent use, and with each components dissolved, stirring, adding distil water is settled to 1000mL, packing then, high temperature sterilization, subsequent use;
(2) preparation of composite nutrient-fluid and use
Mass fraction by each composition; Get potassium peroxydisulfate 3.5 ~ 5.5%, zinc sulphate 4.0 ~ 7.5%, stalk 17.5 ~ 24.0%, corncob 13.5 ~ 18%, yeast powder 1.0 ~ 2.5%, sodium bicarbonate 2.5 ~ 3.5%, water 39 ~ 58.0%, each component is added in proportion, sealing; Fermented 7 ~ 9 days; In strawberry nursery stage, bud stage, vegetative period, it to be mixed in the earth, every day is sooner or later respectively once;
(3) on the strawberry seed, introduce the Semen sesami nigrum chromogene
The strawberry seed is dried in the sun, make that the water content of seed is 10 ~ 15%, be injected among the embryo of strawberry seed, place above-mentioned medium, with 2 ~ 3uw/cm with extracting the Semen sesami nigrum chromogene that obtains in the Semen sesami nigrum
2Behind the seed 15 ~ 30min of UV-irradiation inoculation, put it in the insulating box, constant temperature is handled 16 ~ 18h under 30 ° of C earlier, handles 8 ~ 14h through 35 ° of C constant temperature again, takes out, and naturally cools to room temperature; Transgenosis strawberry seed after handling is moved in the glassware of sterilization treatment, dry under the daylight, making seed moisture content is 10 ~ 15%, and sealing is subsequent use;
(4) grow seedlings
Transgenosis strawberry seed and water is evenly wetting, and the weight of strawberry seed is 100:5 ~ 10 with the ratio of the weight of water, and the transgenic seed after moistening is fully mixed with sulfurous acid iron, and above-mentioned seed is 100:2.5 ~ 5 with the ratio of the quality of sulfurous acid iron; After treatment, place glassware, sealing is sowed behind 10 ~ 14h, grows seedlings.
The strawberry that the present invention prepares is characterized in that, outward appearance is a black, and output is high, and particle is big, and size evenly; Be rich in abundant vitamin E, ferro element, be easy to absorption of human body, biological transformation ratio is high; The brain blood patient that declines there is special curative effect; Under 5 ~ 10 ° of C of low temperature, can preserve 15 ~ 20 days.
The invention has the beneficial effects as follows:
(1). the outward appearance black in color, output is high, and particle is big, and size is evenly;
(2). not only contain abundant vitamin C, B
1, B
2, also contain vitamin E, ferro element, be easy to absorption of human body, biological transformation ratio is high, and patient has special efficacy to cerebral thrombus;
(3). strawberry seedling germination rate is up to 99.5%, and antiviral property, degeneration-resistant border property are strong, under 5 ~ 10 ° of C of low temperature, can preserve 15 ~ 20 days.
?
Embodiment
The raw material that the present invention adopted is: Semen sesami nigrum chromogene, medium, potassium sulphate, iron sulfate, sulfurous acid iron, potassium peroxydisulfate, zinc sulphate, stalk, corncob, water; Pass through gene transfer technique; With the embryo who extracts the Semen sesami nigrum chromogene injection strawberry seed that obtains in the Semen sesami nigrum; And in soil, add compound organic nutritive liquid, and make the strawberry seedling germination rate of cultivating up to 98%, output is high; The outward appearance black in color, particle is big, and size is evenly; Not only contain abundant vitamin C, B
1, B
2, also contain vitamin E, ferro element, be easy to absorption of human body, biological transformation ratio is high, and patient has special efficacy to cerebral thrombus; Antiviral property, degeneration-resistant border property enhancing can be preserved 15 ~ 20 days under 5 ~ 10 ° of C of low temperature.
Instance 1
(1) preparation of medium
In mass, glucose 8g, dipotassium hydrogen phosphate 0.6g, magnesium sulfate 0.1g, agar 12g, nitrate of lime 0.2g, iron sulfate 0.4g, with each components dissolved, stirring, adding distil water is settled to 1000mL, packing then, high temperature sterilization, subsequent use;
(2) preparation of composite nutrient-fluid and use (by the mass fraction of each composition)
By percentage to the quality; Potassium peroxydisulfate 3.5%, zinc sulphate 4.0%, stalk 17.5%, corncob 13.5%, yeast powder 1.0%, sodium bicarbonate 2.5%, water 39% add each component in proportion, sealing; Fermented 7 days; In strawberry nursery stage, bud stage, vegetative period, it to be mixed in the earth, every day is sooner or later respectively once;
(3) on the strawberry seed, introduce the Semen sesami nigrum chromogene
The strawberry seed is dried in the sun, make that the water content of seed is 10%, be injected among the embryo of strawberry seed, place above-mentioned medium, use 2uw/cm extracting the Semen sesami nigrum chromogene that obtains in the Semen sesami nigrum
2Behind the seed 15min of UV-irradiation inoculation, put it in the insulating box, constant temperature is handled 16h under 30 ° of C earlier, handles 8h through 35 ° of C constant temperature again, takes out, and naturally cools to room temperature; Transgenosis strawberry seed after handling is moved in the glassware of sterilization treatment, dry under the daylight, making seed moisture content is 10%, and sealing is subsequent use;
(4) grow seedlings
Transgenosis strawberry seed and water is evenly wetting, and the weight of strawberry seed is 100:5 with the ratio of the weight of water, and the transgenic seed after moistening is fully mixed with sulfurous acid iron, and above-mentioned seed is 100:2.5 with the ratio of the quality of sulfurous acid iron; After treatment, place glassware, sealing is sowed behind the 10h, grows seedlings.
The strawberry outward appearance that obtains is a black, and size is even, and germination rate is 98.6%, under 5 ° of C of low temperature, can preserve 20 days.
Instance 2
(1) preparation of medium
In mass, glucose 10g, dipotassium hydrogen phosphate 0.8g, magnesium sulfate 0.15g, agar 14g, nitrate of lime 0.3g, iron sulfate 0.6g, with each components dissolved, stirring, adding distil water is settled to 1000mL, packing then, high temperature sterilization, subsequent use;
(2) preparation of composite nutrient-fluid and use
By percentage to the quality; Potassium peroxydisulfate 4.6%, zinc sulphate 6.7%, stalk 21.8%, corncob 16.5%, yeast powder 2.0%, sodium bicarbonate 3.0%, water 46.8% add each component in proportion, sealing; Fermented 8 days; In strawberry nursery stage, bud stage, vegetative period, it to be mixed in the earth, every day is sooner or later respectively once;
(3) on the strawberry seed, introduce the Semen sesami nigrum chromogene
The strawberry seed is dried in the sun, make that the water content of seed is 13%, be injected among the embryo of strawberry seed, place above-mentioned medium, use 2.2uw/cm extracting the Semen sesami nigrum chromogene that obtains in the Semen sesami nigrum
2Behind the seed 25min of UV-irradiation inoculation, put it in the insulating box, constant temperature is handled 17h under 30 ° of C earlier, handles 12h through 35 ° of C constant temperature again, takes out, and naturally cools to room temperature; Transgenosis strawberry seed after handling is moved in the glassware of sterilization treatment, dry under the daylight, making seed moisture content is 14%, and sealing is subsequent use;
(4) grow seedlings
Transgenosis strawberry seed and water is evenly wetting, and the weight of strawberry seed is 100:6 with the ratio of the weight of water, and the transgenic seed after moistening is fully mixed with sulfurous acid iron, and above-mentioned seed is 100:4 with the ratio of the quality of sulfurous acid iron; After treatment, place glassware, sealing is sowed behind 10 ~ 14h, grows seedlings.
The strawberry outward appearance that obtains is a black, and size is even, and germination rate is 99.2%, under 7 ° of C of low temperature, can preserve 18 days.
Instance 3
(1) preparation of medium
In mass, glucose 12g, dipotassium hydrogen phosphate 1.0g, magnesium sulfate 0.2g, agar 16g, nitrate of lime 0.5g, iron sulfate 0.8g, with each components dissolved, stirring, adding distil water is settled to 1000mL, packing then, high temperature sterilization, subsequent use;
(2) preparation of composite nutrient-fluid and use
By percentage to the quality; Potassium peroxydisulfate 5.5%, zinc sulphate 7.5%, stalk 24.0%, corncob 18%, yeast powder 2.5%, sodium bicarbonate 3.5%, water 8.0% add each component in proportion, sealing; Fermented 9 days; In strawberry nursery stage, bud stage, vegetative period, it to be mixed in the earth, every day is sooner or later respectively once;
(3) on the strawberry seed, introduce the Semen sesami nigrum chromogene
The strawberry seed is dried in the sun, make that the water content of seed is 115%, be injected among the embryo of strawberry seed, place above-mentioned medium, use 3uw/cm extracting the Semen sesami nigrum chromogene that obtains in the Semen sesami nigrum
2Behind the seed 30min of UV-irradiation inoculation, put it in the insulating box, constant temperature is handled 18h under 30 ° of C earlier, handles 14h through 35 ° of C constant temperature again, takes out, and naturally cools to room temperature; Transgenosis strawberry seed after handling is moved in the glassware of sterilization treatment, dry under the daylight, making seed moisture content is 15%, and sealing is subsequent use;
(4) grow seedlings
Transgenosis strawberry seed and water is evenly wetting, and the weight of strawberry seed is 100:10 with the ratio of the weight of water, and the transgenic seed after moistening is fully mixed with sulfurous acid iron, and above-mentioned seed is 100:5 with the ratio of the quality of sulfurous acid iron; After treatment, place glassware, sealing is sowed behind the 14h, grows seedlings.
The strawberry outward appearance that obtains is a black, and size is even, and germination rate is 99.5%, under 10 ° of C of low temperature, can preserve 15 days.
Claims (2)
1. the cultivation method of a black strawberry, it is characterized in that: outward appearance is a black.
2. according to the cultivation method of the said a kind of black strawberry of claim 1, it is characterized in that specifically preparation process is:
(1) preparation of medium
By the quality of each composition, glucose 8 ~ 12g, dipotassium hydrogen phosphate 0.6 ~ 1.0g, magnesium sulfate 0.1 ~ 0.2g, agar 12 ~ 16g, nitrate of lime 0.2 ~ 0.5g, iron sulfate 0.4 ~ 0.8g; With each components dissolved, stirring, adding distil water is settled to 1000mL, packing, high temperature sterilization, subsequent use;
(2) preparation of composite nutrient-fluid and use
Mass percent by each composition; Potassium peroxydisulfate 3.5 ~ 5.5%, zinc sulphate 4.0 ~ 7.5%, stalk 17.5 ~ 24.0%, corncob 13.5 ~ 18%, yeast powder 1.0 ~ 2.5%, sodium bicarbonate 2.5 ~ 3.5%, water 39 ~ 58.0% add each component in proportion, sealing; Fermented 7 ~ 9 days; In strawberry nursery stage, bud stage, vegetative period, it to be mixed in the earth, every day is sooner or later respectively once;
(3) on the strawberry seed, introduce the Semen sesami nigrum chromogene
The strawberry seed is dried in the sun, make that the water content of seed is 10 ~ 15%, be injected in the strawberry seed, place above-mentioned medium, with 2 ~ 3uw/cm with extracting the Semen sesami nigrum chromogene that obtains in the Semen sesami nigrum
2Behind the seed 15 ~ 30min of UV-irradiation inoculation, put it in the insulating box, constant temperature is handled 16 ~ 18h under 30 ° of C earlier, handles 8 ~ 14h through 35 ° of C constant temperature again, takes out, and naturally cools to room temperature; Transgenosis strawberry seed after handling is moved in the glassware of sterilization treatment, dry under the daylight, making seed moisture content is 10 ~ 15%, and sealing is subsequent use;
(4) grow seedlings
Transgenosis strawberry seed and water is evenly wetting; The weight of strawberry seed is 100:5 ~ 100:10 with the ratio of the weight of water; Transgenic seed after moistening is fully mixed with sulfurous acid iron, and above-mentioned seed is 100:2.5 ~ 100:5 with the ratio of the quality of sulfurous acid iron; After treatment, place glassware, sealing is sowed behind 10 ~ 14h, grows seedlings.
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| CN2012100955884A CN102626049A (en) | 2012-04-02 | 2012-04-02 | Cultivation method of black strawberries |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134989A (en) * | 2015-03-31 | 2016-11-23 | 搴峰嘲 | A kind of breeding method of transgenic melanin Fructus Fragariae Ananssae |
| CN113068612A (en) * | 2021-04-09 | 2021-07-06 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | Method for improving tissue culture germination rate of strawberry seeds |
-
2012
- 2012-04-02 CN CN2012100955884A patent/CN102626049A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134989A (en) * | 2015-03-31 | 2016-11-23 | 搴峰嘲 | A kind of breeding method of transgenic melanin Fructus Fragariae Ananssae |
| CN113068612A (en) * | 2021-04-09 | 2021-07-06 | 贵州省园艺研究所(贵州省园艺工程技术研究中心) | Method for improving tissue culture germination rate of strawberry seeds |
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Application publication date: 20120808 |