CN106134989A - A kind of breeding method of transgenic melanin Fructus Fragariae Ananssae - Google Patents
A kind of breeding method of transgenic melanin Fructus Fragariae Ananssae Download PDFInfo
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Abstract
The present invention relates to the breeding method of a kind of transgenic melanin Fructus Fragariae Ananssae, belong to planting technology field, the method uses Pigment of Black Soya Bean gene, culture medium, sodium thiosulfate, magnesium sulfate, pueraria root residue, starch, sodium sulfate, hypo stone, water to be raw material, pass through gene transfer technique, by in the embryo extracting the Pigment of Black Soya Bean gene injection Fructus Fragariae Ananssae seed obtained in Semen sojae atricolor, and in soil, add the compound organic nutritive liquid containing the trace element such as potassium, ferrum, nurture the Fructus Fragariae Ananssae of the black of a kind of rich in vitamin E, ferrum element.This Fructus Fragariae Ananssae outward appearance is black, and granule is big, and size is uniform；Rich in vitamin E, ferrum element be easily absorbed by the human body, biological transformation ratio is high, and to cerebral thrombosis, patient has special efficacy；Strawberry seedlings germination percentage is up to 99.5%, and antiviral property, degeneration-resistant border property are strong, can preserve 25～30 days at low temperature-5～-10 DEG C.
Description
Technical field
The present invention relates to the breeding method of a kind of transgenic melanin Fructus Fragariae Ananssae, belong to planting technology neck
Territory.
Background technology
Along with the maturation of transgenic technology, promote the development of each industry, with agricultural be especially
Represent.2006, whole world genetically modified crops cultivated area rapidly increased with the speed of 13%,
Breaching 100,000,000 hectares first, most countries has cultivated strawberry in the world.U.S. Fructus Fragariae Ananssae
Cultivated area 2.3 ten thousand hectares, yield 56.7 ten thousand tons, account for the 28% of Gross World Product, flat
All yield 24.6 tons/hectare.Japan cultivated area 1.1 ten thousand hm2, yield 21.8 ten thousand tons,
Account for the 11% of Gross World Product, average product 19.3 ton/hectare.Europe is the main Fructus Fragariae Ananssae place of production,
Account for the 50% of world wide production；The large fruited strawberry cultivation of China starts from 1915, but the most not
Come into one's own, slower development；Since the eighties in 20th century, strawberry production has had rapid development,
China strawberry production area about 70,000 hm2 at present, area ranks first in the world.The present invention based on
Under above background, it is proposed that the breeding method of a kind of transgenic melanin Fructus Fragariae Ananssae.Chinese patent
CN201310033997 discloses the breeding method of a kind of nuisanceless Fructus Fragariae Ananssae, Chinese patent
CN104335891 discloses a kind of New Strawberry Variety breeding method.
Summary of the invention
Present invention aim at: for traditional implantation methods growth cycle length, yield poorly, anti-
The problems such as disease pest ability is low, it is provided that a kind of high yield, be of high nutritive value, resistance turn base
Breeding method because of melanin Fructus Fragariae Ananssae.
In order to reach above-mentioned purpose, the breeding method of a kind of transgenic melanin Fructus Fragariae Ananssae of the present invention,
Comprise the following steps:
Step 1: the preparation of culture medium
Based on the weight portion of each composition, agar 8-12, potassium dihydrogen phosphate 0.6-1.0, chlorination
Magnesium 0.1-0.2, collagen protein 12-16, zinc sulfate 0.2-0.5, sodium chloride 0.4-0.8；
Each component is dissolved, stirring, add distilled water, subpackage, high temperature sterilization, standby；
Step 2: the preparation of composite nutrient-fluid and use
Based on the weight portion of each composition, sodium thiosulfate 3.5-5.5, magnesium sulfate 4.0-7.5,
Pueraria root residue 17.5-24.0, starch 13.5-18, sodium sulfate 1.0-2.5, hypo stone 2.5-3.5,
Water 39-58.0, is proportionally added into each component, seals, ferments 7-9 days, in Fructus Fragariae Ananssae nursery
Phase, bud stage, trophophase, mixed in earth, and every day is the most each once；
Step 3: introduce Pigment of Black Soya Bean gene on Fructus Fragariae Ananssae seed
Fructus Fragariae Ananssae seed is dried in the sun so that the water content of seed is 10-15%, by black
Semen sojae atricolor is extracted the melanin gene obtained be injected in Fructus Fragariae Ananssae seed, be placed in above-mentioned culture medium
In, use 2-3uw/cm2After ultraviolet light irradiates the seed 35-60min of inoculation, put it into constant temperature
In case, first at 45 DEG C, constant temperature processes 20-28h, then processes 10-20h through 55 DEG C of constant temperature, takes
Go out, naturally cool to room temperature；Transgenic Strawberry seed after processing moves into sterilized process
In glass drying oven, drying under daylight, making seed moisture content is 5-10%, seals, standby；
Step 4: nursery
By Transgenic Strawberry seed and water uniform wet, the weight of Fructus Fragariae Ananssae seed and the weight of water it
Ratio is 1000:5-1000:10, and the transgenic seed after moistening is the most mixed with sodium sulfite
Closing, above-mentioned seed is 100:2.5-100:5 with the mass ratio of sodium sulfite；After treatment,
It is placed in glass drying oven, seals, sow after 10-14h, nursery.
The invention provides said method is carried out several further preferably, the first is preferred
Step is:
In step 1:
The weight portion of each composition is, agar 10, potassium dihydrogen phosphate 0.8, magnesium chloride 0.2,
Collagen protein 14, zinc sulfate 0.4, sodium chloride 0.6, distilled water 1000；
In step 2:
The weight portion meter of each composition, sodium thiosulfate 4.5, magnesium sulfate 5.5, pueraria root residue 20,
Starch 16, sodium sulfate 1.5, hypo stone 3.0, water 45.0；
In step 3:
The water content of seed is 12%, is injected into extracting the melanin gene obtained in Semen sojae atricolor
In Fructus Fragariae Ananssae seed, it is placed in above-mentioned culture medium, uses 2-3uw/cm2Ultraviolet light irradiates the kind of inoculation
After sub-50min, putting it in calorstat, first at 45 DEG C, constant temperature processes 24h, then warp
55 DEG C of constant temperature process 15h, take out, naturally cool to room temperature；Transgenic Strawberry after processing
Seed moves in the glass drying oven of sterilized process, dries under daylight, makes the seed moisture content be
7.5%, seal, standby；
In step 4:
By Transgenic Strawberry seed and water uniform wet, the weight of Fructus Fragariae Ananssae seed and the weight of water it
Ratio is 1000:7.5, and the transgenic seed after moistening is sufficiently mixed with sodium sulfite, above-mentioned
Seed is 100:3.5 with the mass ratio of sodium sulfite；After treatment, it is placed in glass drying oven
In, seal, sow after 12h, nursery.
The breeding method of the present invention, the preferred step of the second is:
In step 1:
The weight portion of each composition is, agar 12, potassium dihydrogen phosphate 1.0, magnesium chloride 0.2,
Collagen protein 16, zinc sulfate 0.5, sodium chloride 0.8, distilled water 800；
In step 2:
The weight portion meter of each composition, sodium thiosulfate 5.5, magnesium sulfate 7.5, pueraria root residue 24,
Starch 18, sodium sulfate 2.5, hypo stone 3.5, water 58.0.
The breeding method of the present invention, the third preferred step is:
In step 1:
The weight portion of each composition is, agar 8, potassium dihydrogen phosphate 0.6, magnesium chloride 0.1, glue
Former protein 12, zinc sulfate 0.2, sodium chloride 0.4, distilled water 1200；
In step 2:
The weight portion of each composition is, sodium thiosulfate 3.5, magnesium sulfate 4.0, pueraria root residue 17.5,
Starch 13.5, sodium sulfate 1.0, hypo stone 2.5, water 39, be proportionally added into each component,
Seal, ferment 7-9 days, at Fructus Fragariae Ananssae nursery stage, bud stage, trophophase, mixed earth
In, every day is the most each once.
The invention provides any of the above-described method obtained transgenic melanin seedling of Fructus Fragariae Ananssae
Application, particularly as follows:
(1) selecting physical features higher, ground even, the soil is porous, and irrigation and drainage are organic abundant easily
Loam in plant transgenic melanin seedling of Fructus Fragariae Ananssae；During selection of land, pH value is 5.5-6.5；
(2) mu executes high-quality slightly fertile 2500-3500 weight portion, carbamide 10-20 weight portion, di(2-ethylhexyl)phosphate
Ammonium 15-25 weight portion, potassium sulfate 10-20 weight portion；At trophophase in early spring, topdress 1-2 time,
March before blooming, then impose fertilizer 1-4 time based on phosphorus potassium, when watering, fertilizing, not
Rich water is spattered on flowers and fruits；
(3) temperature 22-28 DEG C, 15-20 DEG C of field planting of ground temperature；Carry out single furrow duplicate rows, seeding row spacing
25 × 25 centimetres, mu needs 5000-6500 strain；20 × 20 specifications；And fill the gaps with seedlings in time: after kind
One week, it is known that whether Seedling survives, check in time and fill a vacancy；Soil is carried out with phoxim baythion or neostigmine
Earth processes with preventing and treating subterranean pest-insect and insect pest in seedling stage；With 70-80% thiophanate methyl wettable powder
600-1000 times of liquid of agent is sprayed, and at individual plant full-bloom stage to the initial stage of bearing pods, every mu sprays
The paclobutrazol aqueous solution 40-60 weight portion of 125ppm；Molten with the potassium dihydrogen phosphate of 0.1-0.3%
Liquid carries out foliage-spray, adds the carbamide of 0.8-1.2%, to strengthen growing way；
(4) will contain melanic DNA be introduced directly into will ripe strawberry plants cell, be aided with height
Then these cells are cultivated and are regenerated plant by the tissue culture system of efficiency in vitro；After go
Except plant cell wall, make protoplast, dipping foreign DNA on metal particle,
Then be used for directly proceed to foreign DNA inject strawberry plants cell, relief strawberry normal
Growth；
(5) until mid-May, it has been the Fructus Fragariae Ananssae outdoor cropping period of maturation, Fructus Fragariae Ananssae fruit now
Real the freshest, in temperature relatively low morning or carry out at dusk plucking Fructus Fragariae Ananssae, Fructus Fragariae Ananssae so can be improved
The fresh-keeping rate of fresh fruit and serviceability rate.
Another of present invention offer is to any of the above-described method obtained transgenic melanin grass
The application of certain kind of berries Seedling, particularly as follows:
(1) selecting physical features higher, ground even, the soil is porous, and irrigation and drainage are organic abundant easily
Loam in plant；During selection of land, pH value is 6；
(2) mu executes the most fertile 3000 weight portions of high-quality, carbamide 15 weight portion, Diammonium phosphate (DAP) 20 weight
Part, potassium sulfate 15 weight portion；At trophophase in early spring, topdress 1-2 time, the March before blooming,
Imposing fertilizer 1-2 time based on phosphorus potassium again, when watering, fertilizing, an other rich water spatters at flowers and fruits
On；
(4) temperature 25 DEG C, 17 DEG C of field planting of ground temperature；Carry out single furrow duplicate rows, seeding row spacing 25 × 25
Centimetre, mu needs 6000 strains；20 × 20 specifications；And fill the gaps with seedlings in time: plant one week after, it is known that Seedling
Whether survive, check in time and fill a vacancy；Soil treatment is carried out with preventing and treating with phoxim baythion or neostigmine
Subterranean pest-insect and insect pest in seedling stage；Spray with 800 times of liquid of 75% thiophanate methyl wettable powder
Mist, at individual plant full-bloom stage to the initial stage of bearing pods, every mu of paclobutrazol aqueous solution 50 spraying 125ppm
Weight portion；Potassium dihydrogen phosphate with 0.2% carries out foliage-spray, adds the carbamide of 1%,
To strengthen growing way.
In a preferred embodiment of the present invention, contain at individual plant in concrete applying step (3)
Florescence to the initial stage of bearing pods, every mu of paclobutrazol aqueous solution 50 kilograms spraying 150ppm.Its effect
More significantly.
In a preferred embodiment of the present invention, in concrete applying step (1), physical features is higher,
Ground even, the soil is porous, and irrigation and drainage are convenient, the sandy loam plantation that illumination is good, finds effect
More notable.
In a preferred embodiment of the present invention, in concrete applying step (3), every mu sprays
The paclobutrazol aqueous solution of 125ppm 50 kilograms；Potassium dihydrogen phosphate with 0.25% carries out leaf
Face sprays, and finds that effect is more notable.
It is that genes of interest enters that genes of interest particle bombardment is introduced directly into recipient cell by the present invention
Entering in recipient cell, and maintain process that is stable and that express in recipient cell, have has
Benefit effect is:
(1) increase yield, trophophase can be shortened but also increase crop yield, make soil obtain
Make full use of to greatest extent；
(2) improvement quality, makes the outward appearance of Fructus Fragariae Ananssae, taste, mouthfeel even nutritional labeling complete
Change；
(3) strengthen resistance, by gene alteration, make tradition strawberry possess and resist disease
The ability of insect pest, greatly reduces pesticide and utilization of pesticides；
(4) produce transgenic medicine, implant melanin, prevent or treat disease.
Specific embodiments
The breeding method of 1 one kinds of transgenic melanin Fructus Fragariae Ananssaes of embodiment, method is:
(1) preparation of culture medium
Based on the quality of each composition, agar 8-12g, potassium dihydrogen phosphate 0.6-1.0g, magnesium chloride
0.1-0.2g, collagen protein 12-16g, zinc sulfate 0.2-0.5g, sodium chloride 0.4-0.8g；
Each component is dissolved, stirring, add distilled water and be settled to 1000mL, subpackage, high temperature sterilization,
Standby；
(2) preparation of composite nutrient-fluid and use
By the mass percent of each composition, sodium thiosulfate 3.5-5.5%, magnesium sulfate
4.0-7.5%, pueraria root residue 17.5-24.0%, starch 13.5-18%, sodium sulfate 1.0-2.5%,
Hypo stone 2.5-3.5%, water 39-58.0%, be proportionally added into each component, seals, fermentation
7-9 days, at Fructus Fragariae Ananssae nursery stage, bud stage, trophophase, being mixed in earth, every day is early
Evening is each once；
(3) on Fructus Fragariae Ananssae seed, Pigment of Black Soya Bean gene is introduced
Fructus Fragariae Ananssae seed is dried in the sun so that the water content of seed is 10-15%, by black
Semen sojae atricolor is extracted the melanin gene obtained be injected in Fructus Fragariae Ananssae seed, be placed in above-mentioned culture medium
In, use 2-3uw/cm2After ultraviolet light irradiates the seed 35-60min of inoculation, put it into constant temperature
In case, first at 45 DEG C, constant temperature processes 20-28h, then processes 10-20h through 55 DEG C of constant temperature,
Take out, naturally cool to room temperature；Transgenic Strawberry seed after processing moves into sterilized process
Glass drying oven in, dry under daylight, making seed moisture content is 5-10%, seal, standby；
(4) nursery
By Transgenic Strawberry seed and water uniform wet, the weight of Fructus Fragariae Ananssae seed and the weight of water it
Ratio is 1000:5-1000:10, and the transgenic seed after moistening is the most mixed with sodium sulfite
Closing, above-mentioned seed is 100:2.5-100:5 with the mass ratio of sodium sulfite；After treatment,
It is placed in glass drying oven, seals, sow after 10-14h, nursery.
This Fructus Fragariae Ananssae outward appearance is black, and granule is big, and size is uniform；Rich in vitamin E, ferrum
Element is easily absorbed by the human body, and biological transformation ratio is high, and to cerebral thrombosis, patient has special efficacy；Fructus Fragariae Ananssae
Seedling germination percentage is up to 99.5%, and antiviral property, degeneration-resistant border property are strong, low temperature-5--10 DEG C
Under can preserve 25-30 days.
Embodiment 2
The most wholely: Fructus Fragariae Ananssae has happiness light, Xi Shui, happiness is fertile, be afraid of the feature of flood, selects physical features
Higher, ground even, the soil is porous, plants in irrigation and drainage organic abundant loam easily,
Fructus Fragariae Ananssae happiness neutrality or subacidity soil, during selection of land, pH value is 6；
Use sufficient base fertilizer: general mu executes high-quality slightly fertile 3000 kilograms, 15 kilograms of carbamide, di(2-ethylhexyl)phosphate
Ammonium 20 kilograms, potassium sulfate 15 kilograms；At trophophase in early spring, topdress 1-2 time, before blooming
March, then impose fertilizer 1-2 time based on phosphorus potassium, when watering, fertilizing, an other rich water spatters
On flowers and fruits；
Plant is planted: temperature 25 DEG C, 17 DEG C of field planting of ground temperature；The method of kind: single furrow duplicate rows, strain
Line-spacing 25 × 25 centimetres, mu needs 6000 strains；20 × 20 specifications；Fill the gaps with seedlings in time: after kind one
Week, it is known that whether Seedling survives, and should check in time and fill a vacancy, with phoxim baythion or newly execute understand carry out soil
Earth processes with preventing and treating subterranean pest-insect and insect pest in seedling stage；With 75% thiophanate methyl wettable powder
800 times of liquid are sprayed, and at individual plant full-bloom stage to the initial stage of bearing pods, every mu sprays 125ppm's
Paclobutrazol aqueous solution 50 kilograms；Potassium dihydrogen phosphate with 0.2% carries out foliage-spray, adds
Enter the carbamide of 1%, to strengthen growing way；
Type of rearing: directly proceed to method, will contain that melanic DNA is introduced directly into will ripe grass
Certain kind of berries plant cell, is aided with high efficiency tissue culture system, then by these cells in vitro
Cultivation regenerates plant, removes plant cell wall afterwards, makes protoplast, micro-at metal
Dipping foreign DNA on grain, is then used for directly proceeding to foreign DNA and injects strawberry plants cell,
Relief strawberry normal growth；
Gather in time: until mid-May, be the Fructus Fragariae Ananssae outdoor cropping period of maturation, now
Strawberry fruit fresh, in temperature relatively low morning or carry out plucking Fructus Fragariae Ananssae at dusk, the most permissible
Improve fresh-keeping rate and the serviceability rate of Fructus Fragariae Ananssae fresh fruit.
Embodiment 3
Substantially the same manner as Example 2, at individual plant full-bloom stage to the initial stage of bearing pods, every mu sprays
The paclobutrazol aqueous solution of 150ppm 50 kilograms.Its effect is more significantly.
Embodiment 4
Substantially the same manner as Example 2, select physical features higher, ground even, the soil is porous, row
Fill convenient, the sandy loam plantation that illumination is good, find that effect is more notable.
Embodiment 5
Substantially the same manner as Example 2, except for the difference that every mu of paclobutrazol spraying 125ppm is water-soluble
Liquid 50 kilograms；Potassium dihydrogen phosphate with 0.25% carries out foliage-spray, finds effect more
Significantly.
Embodiment described above only the preferred embodiment of the present invention is described, the most right
The scope of the present invention is defined, on the premise of designing spirit without departing from the present invention, and this area
Various deformation that technical scheme is made by those of ordinary skill and improvement, all should fall into
In the protection domain that claims of the present invention determines.
Claims (9)
1. the breeding method of a transgenic melanin Fructus Fragariae Ananssae, it is characterised in that comprise the following steps:
Step 1: the preparation of culture medium
Based on the weight portion of each composition, agar 8～12, potassium dihydrogen phosphate 0.6～1.0, magnesium chloride 0.1～0.2,
Collagen protein 12～16, zinc sulfate 0.2～0.5, sodium chloride 0.4～0.8；Each component is dissolved, stirs, add steaming
Distilled water, subpackage, high temperature sterilization, standby；
Step 2: the preparation of composite nutrient-fluid and use
Based on the weight portion of each composition, sodium thiosulfate 3.5～5.5, magnesium sulfate 4.0～7.5, pueraria root residue
17.5～24.0, starch 13.5～18, sodium sulfate 1.0～2.5, hypo stone 2.5～3.5, water 39～58.0, by each group
Divide and be proportionally added into, seal, ferment 7～9 days, at Fructus Fragariae Ananssae nursery stage, bud stage, trophophase, mixed
In earth, every day is the most each once；
Step 3: introduce Pigment of Black Soya Bean gene on Fructus Fragariae Ananssae seed
Fructus Fragariae Ananssae seed is dried in the sun so that the water content of seed is 10～15%, will Semen sojae atricolor extract
The melanin gene obtained is injected in Fructus Fragariae Ananssae seed, is placed in above-mentioned culture medium, with 2～3uw/cm2Ultraviolet light
After irradiating the seed 35～60min of inoculation, putting it in calorstat, first at 45 DEG C, constant temperature processes 20～28h,
Process 10～20h through 55 DEG C of constant temperature again, take out, naturally cool to room temperature；Transgenic Strawberry kind after processing
Son moves in the glass drying oven of sterilized process, dries under daylight, and making seed moisture content is 5～10%, seals,
Standby；
Step 4: nursery
By Transgenic Strawberry seed and water uniform wet, the weight of Fructus Fragariae Ananssae seed and the weight ratio of water are 1000:
5～1000:10, the transgenic seed after moistening is sufficiently mixed with sodium sulfite, above-mentioned seed and sodium sulfite
Mass ratio be 100:2.5～100:5；After treatment, it is placed in glass drying oven, seals, after 10～14h
Sowing, nursery.
2. breeding method as claimed in claim 1, it is characterised in that comprise the following steps:
In described step 1:
The weight portion of each composition is, agar 10, potassium dihydrogen phosphate 0.8, magnesium chloride 0.2, collagen protein 14,
Zinc sulfate 0.4, sodium chloride 0.6, distilled water 1000；
In described step 2:
The weight portion meter of each composition, sodium thiosulfate 4.5, magnesium sulfate 5.5, pueraria root residue 20, starch 16, sulfur
Acid sodium 1.5, hypo stone 3.0, water 45.0；
In described step 3:
The water content of described seed is 12%, is injected into Fructus Fragariae Ananssae kind by extracting the melanin gene obtained in Semen sojae atricolor
In son, it is placed in above-mentioned culture medium, with 2～3uw/cm2After ultraviolet light irradiates the seed 50min of inoculation, by it
Putting in calorstat, first at 45 DEG C, constant temperature processes 24h, then processes 15h through 55 DEG C of constant temperature, takes out, natural
It is cooled to room temperature；Transgenic Strawberry seed after processing moves in the glass drying oven of sterilized process, under daylight
Drying, making seed moisture content is 7.5%, seals, standby；
In described step 4:
By Transgenic Strawberry seed and water uniform wet, the weight of Fructus Fragariae Ananssae seed and the weight ratio of water are 1000:
7.5, the transgenic seed after moistening is sufficiently mixed with sodium sulfite, above-mentioned seed and the quality of sodium sulfite
Ratio be 100:3.5；After treatment, it is placed in glass drying oven, seals, sow after 12h, nursery.
3. breeding method as claimed in claim 1, it is characterised in that comprise the following steps:
In described step 1:
The weight portion of each composition is, agar 12, potassium dihydrogen phosphate 1.0, magnesium chloride 0.2, collagen protein 16,
Zinc sulfate 0.5, sodium chloride 0.8, distilled water 800；
In described step 2:
The weight portion meter of each composition, sodium thiosulfate 5.5, magnesium sulfate 7.5, pueraria root residue 24, starch 18, sulfur
Acid sodium 2.5, hypo stone 3.5, water 58.0.
4. breeding method as claimed in claim 1, it is characterised in that comprise the following steps:
In described step 1:
The weight portion of each composition is, agar 8, potassium dihydrogen phosphate 0.6, magnesium chloride 0.1, collagen protein 12,
Zinc sulfate 0.2, sodium chloride 0.4, distilled water 1200；
In described step 2:
The weight portion of each composition is, sodium thiosulfate 3.5, magnesium sulfate 4.0, pueraria root residue 17.5, starch 13.5,
Sodium sulfate 1.0, hypo stone 2.5, water 39, be proportionally added into each component, seals, ferments 7～9 days,
Fructus Fragariae Ananssae nursery stage, bud stage, trophophase, mixed in earth, and every day is the most each once.
5. the application of the arbitrary described transgenic melanin seedling of Fructus Fragariae Ananssae of claim 1-4, it is characterised in that its application
Method is:
1) selecting physical features higher, ground even, the soil is porous, plants in irrigation and drainage organic abundant loam easily
Described transgenic melanin seedling of Fructus Fragariae Ananssae；During selection of land, pH value is 5.5-6.5；
2) mu execute high-quality slightly fertile 2500-3500 weight portion, carbamide 10-20 weight portion, Diammonium phosphate (DAP) 15-25 weight portion,
Potassium sulfate 10-20 weight portion；At trophophase in early spring, topdress 1～2 time, the March before blooming, then impose with phosphorus
Potassium is the fertilizer 1～4 times led, and when watering, fertilizing, an other rich water spatters on flowers and fruits；
3) temperature 22-28 DEG C, 15-20 DEG C of field planting of ground temperature；Carry out single furrow duplicate rows, seeding row spacing 25 × 25 centimetres, mu
Need 5000-6500 strain；20 × 20 specifications；And fill the gaps with seedlings in time: plant one week after, it is known that whether Seedling survives, in time
Inspection is filled a vacancy；Soil treatment is carried out with preventing and treating subterranean pest-insect and insect pest in seedling stage with phoxim baythion or neostigmine；With
70-80% 600-1000 times of liquid of thiophanate methyl wettable powder is sprayed, at individual plant full-bloom stage at the beginning of bearing pods
Phase, every mu of paclobutrazol aqueous solution 40-60 weight portion spraying 125ppm；With the potassium dihydrogen phosphate of 0.1-0.3%
Solution carries out foliage-spray, adds the carbamide of 0.8-1.2%, to strengthen growing way；
4) will contain melanic DNA be introduced directly into will ripe strawberry plants cell, be aided with high efficiency tissue
Then these cells are cultivated and are regenerated plant by culture systems in vitro；Rear removal plant cell wall, is allowed to into
For protoplast, on metal particle, dipping foreign DNA, is then used for directly proceeding to foreign DNA and injects
Strawberry plants cell, relief strawberry normal growth；
5) until mid-May, being the Fructus Fragariae Ananssae outdoor cropping period of maturation, strawberry fruit now is fresh, in temperature
Spend relatively low morning or carry out at dusk plucking Fructus Fragariae Ananssae, so can improve fresh-keeping rate and the serviceability rate of Fructus Fragariae Ananssae fresh fruit.
Application the most according to claim 5, its application process is:
1) selecting physical features higher, ground even, the soil is porous, plants in irrigation and drainage organic abundant loam easily；
During selection of land, pH value is 6；
2) mu executes the most fertile 3000 weight portions of high-quality, carbamide 15 weight portion, Diammonium phosphate (DAP) 20 weight portion, potassium sulfate
15 weight portions；At trophophase in early spring, topdress 1～2 time, the March before blooming, then impose based on phosphorus potassium
Fertilizer 1～2 times, when watering, fertilizing, an other rich water spatters on flowers and fruits；
3) temperature 25 DEG C, 17 DEG C of field planting of ground temperature；Carrying out single furrow duplicate rows, seeding row spacing 25 × 25 centimetres, mu needs 6000
Strain；20 × 20 specifications；And fill the gaps with seedlings in time: plant one week after, it is known that whether Seedling survives, check in time and fill a vacancy；With
Phoxim baythion or neostigmine carry out soil treatment with preventing and treating subterranean pest-insect and insect pest in seedling stage；Use 75% thiophanate methyl
800 times of liquid of wettable powder are sprayed, and at individual plant full-bloom stage to the initial stage of bearing pods, every mu sprays 125ppm's
Paclobutrazol aqueous solution 50 weight portion；Potassium dihydrogen phosphate with 0.2% carries out foliage-spray, adds 1%
Carbamide, to strengthen growing way.
Application the most according to claim 6, it is characterised in that at individual plant full-bloom stage to the initial stage of bearing pods, every mu
Spray paclobutrazol aqueous solution 50 weight portion of 150ppm.
Application the most according to claim 6, it is characterised in that described soil is husky earth.
Application the most according to claim 6, it is characterised in that every mu of paclobutrazol spraying 125ppm is water-soluble
Liquid 50 weight portion；Potassium dihydrogen phosphate with 0.25% carries out foliage-spray.
Priority Applications (1)
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| CN110583139A (en) * | 2018-06-13 | 2019-12-20 | 郭睿 | Method for improving germination rate of strawberry seeds |
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| CN102626049A (en) * | 2012-04-02 | 2012-08-08 | 常州亚环环保科技有限公司 | Cultivation method of black strawberries |
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| CN102626049A (en) * | 2012-04-02 | 2012-08-08 | 常州亚环环保科技有限公司 | Cultivation method of black strawberries |
| CN103931403A (en) * | 2014-04-30 | 2014-07-23 | 重庆市姚天虎水果种植股份合作社 | Strawberry outdoor cultivation method |
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| CN110583139A (en) * | 2018-06-13 | 2019-12-20 | 郭睿 | Method for improving germination rate of strawberry seeds |
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