CN102559731B - 一种假病毒载体及其制备方法和应用 - Google Patents
一种假病毒载体及其制备方法和应用 Download PDFInfo
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CN105734046B (zh) * | 2014-12-12 | 2019-04-26 | 中国检验检疫科学研究院 | 无细胞表达系统制备Armored RNA的方法 |
CN104711373A (zh) * | 2015-04-03 | 2015-06-17 | 张瑾 | 耐RNase的登革病毒核酸检测质控品的制备方法 |
US20180127837A1 (en) * | 2015-12-22 | 2018-05-10 | Chinese Academy Of Inspection And Quarantine | Construction and application of one innovative expression vector for virus-like particles |
CN105907727A (zh) * | 2016-05-03 | 2016-08-31 | 中国水产科学研究院黄海水产研究所 | 内含三种食源性病毒核酸片段的假病毒颗粒及其制备方法 |
CN106011078A (zh) * | 2016-05-03 | 2016-10-12 | 中国水产科学研究院黄海水产研究所 | 内含轮状病毒核酸片段的假病毒颗粒及其制备方法 |
CN105907726A (zh) * | 2016-05-03 | 2016-08-31 | 中国水产科学研究院黄海水产研究所 | 内含甲型肝炎病毒核酸片段的假病毒颗粒及其制备方法 |
CN105950565B (zh) * | 2016-05-03 | 2019-08-16 | 中国水产科学研究院黄海水产研究所 | 高稳定性假病毒颗粒及其制备所用的质粒载体和方法 |
CN106085974B (zh) * | 2016-06-07 | 2019-08-09 | 博奥生物集团有限公司 | 一种寨卡病毒假病毒颗粒及其制备方法 |
CN105969786B (zh) * | 2016-06-07 | 2019-08-02 | 博奥生物集团有限公司 | 一种表达ms2噬菌体衣壳蛋白和成熟酶的质粒 |
CN110964849B (zh) * | 2019-11-22 | 2020-08-04 | 华南农业大学 | 一种消除非洲猪瘟病毒检测假阳性的方法及检测非洲猪瘟病毒的试剂盒 |
WO2021180139A1 (zh) * | 2020-03-10 | 2021-09-16 | 广州复能基因有限公司 | 用于检测来自dna或rna生物体的样品靶标的可掺入参照标准品 |
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Non-Patent Citations (5)
Title |
---|
H5N1 型禽流感病毒核蛋白C 端缺失削弱核蛋白间的相互作用;李斌等;《生物技术通讯》;20090131;第20卷(第1期);19-21 * |
MS2噬菌体壳蛋白载动的HIV反链基因阻断病毒蛋白质合成;吴敏;《中华徽生物学和免疫学杂志》;19941031;第14卷(第5期);292-295 * |
吴敏.MS2噬菌体壳蛋白载动的HIV反链基因阻断病毒蛋白质合成.《中华徽生物学和免疫学杂志》.1994,第14卷(第5期),292-295. |
李斌等.H5N1 型禽流感病毒核蛋白C 端缺失削弱核蛋白间的相互作用.《生物技术通讯》.2009,第20卷(第1期),19-21. |
陆学东,等.HIV-1 gag基因p17+24重组抗原表达、纯化及抗原性分析.《中华微生物学和免疫学杂志》.2007,第27卷(第7期),612-614. * |
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