CN102553904B - Bioremediation method for soil polluted by heavy metals - Google Patents
Bioremediation method for soil polluted by heavy metals Download PDFInfo
- Publication number
- CN102553904B CN102553904B CN 201210014161 CN201210014161A CN102553904B CN 102553904 B CN102553904 B CN 102553904B CN 201210014161 CN201210014161 CN 201210014161 CN 201210014161 A CN201210014161 A CN 201210014161A CN 102553904 B CN102553904 B CN 102553904B
- Authority
- CN
- China
- Prior art keywords
- earthworm
- soil
- days
- plant
- earthworms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a bioremediation method for soil polluted by heavy metals. The bioremediation method comprises the following steps of: adding earthworm feeds into the soil polluted by the heavy metals, turning the soil, uniformly mixing, and putting earthworms into the soil and cultivating the earthworms; after the earthworms are cultivated for 28 to 35 days, collecting earthworm excrements in the upper layer soil, collecting adult earthworms by adopting a natural lighting method, and retaining young earthworms and earthworm eggs in the soil; planting plants by adopting a hedge planting method, cradling the parts of the plants growing for 78 to 90 days, which are positioned above the ground, and removing the whole plants after the plants grow for another 50 to 70 days; collecting the adult earthworms and the earthworm excrements for 1 to 3 days by adopting an outdoor collection method every 28 to 35 days during planting of the plants; and after the whole plants are removed, collecting all the adult earthworms and the young earthworms by adopting the natural lighting method, and collecting the earthworm excrements and the earthworm eggs. The soil is subjected to remediation by combining animals, plants and microorganisms according to a proper remediation sequence and a remediation period, so that the bioremediation method is economic, environment-friendly, easy to operate, high in remediation efficiency and easy to popularize, and is particularly suitable for mild or moderate copper, cadmium and lead combined polluted soils.
Description
Technical field
The present invention relates to the environmental pollution treatment technology field, relate in particular to a kind of biological renovation method of heavy-metal contaminated soil.
Background technology
Along with the development of industrial and agricultural production, heavy metal pollution of soil is further serious, particularly Single Pollution or the combined pollution of copper, cadmium, plumbous this three heavy metal species.Heavy metal pollution of soil is one of subject matter that affects human health and environmental quality, and it not only affects crops production, and affects atmosphere and quality of water environment, even passes through the health of food chain harm humans.Therefore, heavy metal-polluted soil copper, cadmium, plumbous pollution are badly in need of administering.
At present, broad research is all being carried out to the heavy metal pollution of soil recovery technique in countries in the world, and its main path has 3 kinds: 1. change the heavy metal state, reduce its activity, make its passivation, to reduce toxicity; 2. with engineering, heavy metal is become soluble state, free state, then pass through drip washing, then reclaim, to reduce the content of beary metal in soil; 3. utilize the heavy metal in hyperaccumulative plant absorption soil, then harvesting, reach the purpose that removes the heavy metal in soil.Concrete operation method generally has following several: (1) soil moved in to improve the original method, soil replacement method and deep ploughing are digged; (2) electronic reparation and electric heating reparation; (3) chemical leaching; (4) chemistry is repaired, and comprises chemical improvement agent, surfactant and heavy metal antagonist reparation; (5) biological restoration comprises that phytoremediation, animal are repaired and microorganism is repaired.Front four kinds of common costs of method are higher, some easy spoiled soil structure also, and biological restoration is few to soil disturbance, cost is low and can large scale application, therefore received increasing concern.
Biological restoration is that the biotechnology of utilizing that grows up early 1990s is administered a kind of new method of contaminated soil.Its basic principle is the toxicity of utilizing the heavy metal in specific plant, animal or microorganism reduction, purification soil or reducing heavy metal.The method is a kind of original position reparation, and cost is low, and is effective, and easy operating is subject to people's attention day by day, becomes the focus of contaminated soil reparation research.What application was more at present is that plant, microbial association reparation or animal are repaired, and utilizes the fewer of animal, plant and microbial association reparation.
Earthworm as typical soil animal, is referred to as " expert of improvement soil ".Studies confirm that earthworm can improve on the one hand soil texture and fertility, promotion plant root growth, can improve quantity and the activity of edaphon by external secretion and earthworm excrement on the other hand, earthworm is successfully applied in domestic waste, Treatment of Sludge.
Bai Sanye (Trifolium repens) is the pulse family perennial herb.Stem is crawled, and palmately compound leaf has 3 leaflets, leaflet obovate or reversed cardioid, inflorescence head, corolla white.Bai Sanye conduct herbage commonly used, wide adaptability, cultivation technique is ripe, is beneficial to utilization and extention.
Rye grass (Lolium perenne) is the grass family perennial herb.The leaf bar shaped, the stem stalk is grown thickly, and quality is softer.Rye grass is the tame forage grass with economic worth, wide adaptability, and cultivation technique is ripe, is beneficial to and applies.
Dayflower (Commelina communis) is the Commelianaceae annual herb, leaf be lanceolar to ovum shape lanceolar, stem is crawled, give birth on flower top or armpit is given birth to, the flower blue and white.Also be wild state before Commelinales, develop very few.
Summary of the invention
The invention provides a kind of biological renovation method of heavy-metal contaminated soil, the method economical rationality, simple to operate, repairing effect is good, is easy to promote and environmental protection.
A kind of biological renovation method of heavy-metal contaminated soil comprises:
(1) apply earthworm feedstuff in heavy-metal contaminated soil, the mixing of digging is put into vermiculture; Cultivate after 28-35 days, collect the earthworm excrement in upper layer of soil, then adopt the natural lighting method to be gathered into earthworm, young earthworm and earthworm ovum are stayed in soil;
(2) adopt the mode planting plants of hedge planting, plant growth cradles its aerial part during to 78-90 days, plant integral body is removed in regrowth 50-70 days;
During planting plants, every 28-35 days, adopt open-air collecting method to be gathered into earthworm 1-3 days; After plant integral body removes, adopt the natural lighting method to collect all and become earthworm, young earthworm, and collect earthworm excrement and earthworm ovum;
Wherein, during planting plants, described plant is Bai Sanye (Trifolium repens) and dayflower (Commelina communis), two Plants interval hedge plantings when spring or summer; Described Bai Sanye is with the rear sowing plantation of rhizobium seed dressing;
When autumn or winter planting plant, described plant is rye grass (Lolium perenne).
The method that the present invention adopts animal, plant, microbial association to repair is repaired heavy-metal contaminated soil.
Step (1) is the animal repairing phase, and repair process continues 28-35 days.
In this step, described heavy metal can be one or more in cadmium, copper and lead.
Described heavy-metal contaminated soil can cause Heavy metal contaminated soil for the long-term electronic product of stacking.
Described heavy-metal contaminated soil can be moderate or slight pollution soil; Preferably, described heavy-metal contaminated soil is intermediate pollution soil, contains the cadmium that concentration is 0.6-3.6mg/kg, copper and the concentration that concentration is 86.2-135.4mg/kg is the lead of 67.1-154.8mg/kg.
Described earthworm feedstuff is preferably lemon dregs.Fertilizer commonly used such as cow dung, pig manure, chicken manure etc., due in the animal feeding process take the feeding feed as main, so in fertilizer, content of beary metal is usually higher, can affect the progress of soil remediation; And lemon dregs is the byproduct that obtains after corn or cassava fermentation production of citric acid, and its content of beary metal is low after testing, and it is the Cu of 7mg/kg that content is only arranged, the content of Cd and Pb is 0, simultaneously, lemon dregs is also one of feed of enjoying a lot of earthworm, is beneficial to the growth of earthworm.
The applied amount of described lemon dregs is preferably 0.8-1.5kg/m
2Applying digs after lemon dregs evenly can make lemon dregs disperse, and is convenient to earthworm and disperses to look for food.
Described earthworm is preferably large flat No. two earthworms.The earthworm fast growth of this earthworm kind, fertility is strong, and heavy metal copper, cadmium, lead are had stronger patience.
The input density of described large flat No. two earthworms is preferably 0.5-0.6kg/m
2
According to the habit of earthworm excretion earthworm excrement, described earthworm excrement is generally concentrated and is distributed in topsoil, by collecting this layer soil, can collect most earthworm excrement.
Described natural lighting method can be as follows: under natural lighting, soil is successively pushed aside, subsoil is sieved, isolate one-tenth earthworm wherein, soil is returned paving after being gathered into earthworm.Because earthworm has photophobism, adopt the natural lighting method can make earthworm concentrate on the soil bottom, collect subsoil after sieve, just can isolate into earthworm, young earthworm and earthworm ovum to be stayed in soil, follow-up the need thrown in again.
Step (2) is animal, plant, microbial association repairing phase, and repair process continues 128-160 days.
In this step, preferably, the planting patterns of described dayflower is seedling cultivation; The planting patterns of Bai Sanye and rye grass is the sowing plantation.Dayflower is due to the seed collection difficulty, but its vegetative propagation coefficient is higher, therefore adopt seedling cultivation; Bai Sanye and rye grass easily survive because seed is easily collected, and can directly sow plantation, and be easy to operate, can save a large amount of human resources.
Plantation again after described Bai Sanye dresses seed with rhizobium.Because Bai Sanye is legume, the seed dressing rhizobium are conducive to strengthen its nitrogen fixing capacity, promote its growth, promote it to the absorption of heavy metal; Simultaneously, the activity of rhizobium can change the microenvironment of root system of plant, makes the heavy metal in soil change exchangeable species into, or makes the Heavy Metals of high poison change the low toxicity state into, is more conducive to the plant and animal enrichment.
Preferably, described Bai Sanye cradles rear toothing 3-5cm, and dayflower cradles rear toothing 12-18cm, and rye grass cradles rear toothing 6-8cm, is convenient to the plant continued growth.
Described open-air collecting method can be as follows: lay at the heavy-metal contaminated soil edge that to contain concentration be 3-5kg/m
2The soil of earthworm feedstuff was collected one-tenth earthworm and the earthworm excrement of laying in soil in 1-2 days afterwards, and young earthworm and earthworm ovum are stayed in soil, afterwards this soil was backfilling in heavy-metal contaminated soil.Laying contains the soil of high concentration earthworm feedstuff, earthworm can be attracted to come, and is convenient to collect.
The described time that is gathered into earthworm is 1-3 days, and under this condition, collected earthworm quantity is suitable.Acquisition time is long, and the earthworm in native soil can focus in a large number lays in soil, can cause earthworm lazy weight in native soil.
In whole biological restoration process, in order to reduce the impact of rainwater, convenient insulation, preferably carry out in beam-connected greenhouse simultaneously.
In described vermiculture process, apply weekly earthworm feedstuff one time, the applied amount of earthworm feedstuff is preferably 0.5-0.6kg/m
2
Preferably, in time water in vermiculture and planting process, make the water content of heavy-metal contaminated soil remain on 70-85%.
Described heavy-metal contaminated soil surface can be coated with straw or stalk, is convenient to moisturizing.
The described soil moisture is preferably 5-25 ℃.When temperature is too low, can be incubated by covered with plastic film; During excess Temperature, can the cooling of coverage shading net.
In the present invention, earthworm by wriggling, taking food and can improve soil texture, fertility, moisture, nutrient and aeration status, promotes plant root growth on the one hand, can improve quantity and the activity of edaphon by external secretion and earthworm excrement on the other hand.Find through preliminary test, flat greatly No. two earthworm counterweight metals have enriched character, and earthworm vermis and earthworm excrement content of beary metal are higher in the higher soil of content of beary metal, have heavy-metal contaminated soil reparative potential preferably.Breeding earthworm in soil, earthworm regularly is gathered into earthworm, earthworm excrement by passive diffusion and the effect enriching heavy metal of ingesting, then carries out ashing and process, and can remove the heavy metal in soil.
Because different plants have different growth periods, in order to carry out the reparation of soil throughout the year, choose different plants in Various Seasonal and plant.Premenstruum (premenstrua), research was found, Bai Sanye, dayflower and rye grass all have good repairing effect to the soil of cadmium, copper, plumbous combined pollution, energy Sync enrichment cadmium, copper, plumbous three kinds of metallic elements.This three Plants is in self growth course, can simultaneously the heavy metal in soil be absorbed, be enriched in the aerial part or under ground portion of plant, cradle when the aerial growth of plant reaches certain biomass, the heavy metal that is enriched in above-ground plant parts is removed along with the harvesting of aerial part; This stage when finishing removes plant integral body, can remove all heavy metals of enrichment in whole strain plant.Cradle at every turn and obtain the plant that part and last integral body removes carry out ashing after drying, process as hazardous waste, can effectively remove thus the heavy metal in soil.
With the three leaf seed dressings of rhizobium dialogue, Bai Sanye is legume, and the seed dressing rhizobium are conducive to strengthen its nitrogen fixing capacity, promote its Fast Growth and breeding; The activity of rhizobium has simultaneously changed the root system of plant microenvironment, makes the heavy metal in soil be transformed into exchangeable species, or makes the Heavy Metals of high poison be transformed into the low toxicity state, is beneficial to the plant and animal enrichment.In addition, soil itself contains abundant microbial population, can degrade to heavy metal, and the excreta earthworm fecal energy of earthworm improves validity biological in soil, improves the activity of microorganism, accelerated degradation.
In order to improve the heavy-metal contaminated soil remediation efficiency, to promote the reparation process, can be half a year in cycle, interim weekly, advanced action thing reparation (continuing 28-35 days), then carry out animal, plant and microbial association reparation (continuing 128-160 days).According to the Heavy metal pollution of soil degree, carry out a plurality of repairing efficiencies, the content of beary metal in soil is descended gradually, thereby reach environmental safety standard.The inventive method is particularly suitable for the reparation of slight or moderate heavy-metal contaminated soil, to slight pollution soil, repairs 1-2 cycle to get final product; The alignment degree contaminated soil is repaired 2-5 cycle to get final product.
The present invention adopts animal, plant and microbial association, by suitable reparation order and repairing efficiency, realizes the reparation to heavy-metal contaminated soil, has following beneficial effect:
(1) adopt restoration of soil polluted by heavy metal of the present invention, heavy metal copper, cadmium, lead in energy Sync enrichment soil, composite governance is effective, and enriching quantity is considerable.
(2) peaceful No. two earthworms growth is fast, and breeding coefficient is large, and breeding is than reaching 1: 1500, and namely disposable input earthworm is 100, and year can be bred 150,000, and heavy metal copper, cadmium, lead are had stronger patience, and copper in vermis and earthworm excrement, cadmium, lead content are considerable.
(3) plant of the present invention is the respond well plant of heavy metal accumulation that screens in research process.Wherein, Bai Sanye, rye grass are the herbage of commonly using, and biomass is large, easily cultivation, and fertility is strong, and fast growth can adopt extensive management in growth course, and simple to operate, management cost is lower, and is applied widely, easily promotes; Though dayflower is wild plant, its open-air plant amount is large, and brood body easily gathers, and breeding easily.
(4) activity of animal, microorganism has improved soil environment effectively, has promoted the growth of plant, has improved the enrichment of plant to heavy metal, and synergy is remarkable, has effectively shortened repairing efficiency.Use the present invention, soil content of beary metal in 1-2 of middle low copper, cadmium, plumbous combined pollution is significantly reduced, reach smoothly the requirement of safety in production, remediation efficiency is high, the improvement that is specially adapted to plough.
The specific embodiment
Embodiment 1
Test site is located at Taizhou one electronic waste and stacks for a long time the plot, and this plot soil thickness 10-25cm is mainly cadmium, copper, plumbous combined pollution.
According to even exercisable principle, the plot is divided into the sample ground of 10m * 10m, take each sample ground as the unit collection of layouting, to realize the typicalness of data.
Take a sample ground as unit, select 5 points each sample, adopt the quincunx method of layouting, get 4 points with radial again around the central point of choosing.Gather soil (0-20cm) at each point respectively with soil sampler, then 5 soil are mixed, pick in the numbered plastic packaging bag of tool of packing into after the foreign material such as stone, plant residue, as a soil sample.Soil sample is taken back rear air-dry, and soil block is pulverized, got cross in right amount after 100 mesh sieves standby.
Soil detects: the soil sample of fetching is detected, and pre-treatment and detection method are with reference to " standard of soil environment quality " (GB15618-1995) described execution, and testing result sees Table 1.
Heavy metal pollution of soil situation before table 1 is repaired
| Main polluted heavy metals | Cd(mg/kg) | Cu(mg/kg) | Pb(mg/kg) |
| Content before repairing | 0.6-3.6 | 86.2-135.4 | 67.1-154.8 |
For above-mentioned contaminated soil, 3 repairing efficiencies of the present embodiment design specifically comprise following 6 repairing phases:
Phase I: animal is repaired, and the duration is 1 month;
Second stage: animal, plant and microbial association reparation, the duration is 5 months;
Phase III: animal is repaired, and the duration is 1 month;
The quadravalence section: animal, plant and microbial association reparation, the duration is 5 months;
Five-stage: animal is repaired, and the duration is 1 month;
The 6th stage: animal, plant and microbial association reparation, the duration is 5 months.
Before reparation, build simple and easy beam-connected greenhouse repairing on the place, district, and restoring area is made the ridge shape, facilitate earthworm to throw in and planting, row spacing 1.2m, ground, length visual field and deciding.
Phase I, animal repair process: (since on February 22nd, 2010)
1) with the soil scarifying, apply earthworm food lemon dregs in the process of turning over, 1kg/m
2, make it as far as possible and the soil mixing, and cover next year old straw of one deck at soil surface, be used for moisturizing, as temperature during lower than 5 ℃, the covered with plastic film insulation during higher than 25 ℃, covers 20% shading screen cooling when temperature.
2) put into earthworm (flat greatly No. two earthworms) cultivation, input density is 0.5kg/m
2, earthworm ingests by ceaselessly wriggling, and soil is taken in body together, makes heavy metal enrichment in vivo.Keep the skin wet according to weather condition during this time, on average watered one time water in 1-2 days; In the earthworm bioaccumulation process weekly feeding once, adopt surface trench digging feeding, throw in lemon dregs 0.5kg/m at every turn
2
3) collect vermis, adopt the natural lighting method, watered in 24 hours in advance, soil with the upper strata concentrates on cement flooring or Polypropylence Sheet again, successively push aside, soil is scratched clean, make earthworm concentrate on bottom, reach the collection purpose, and will become earthworm vermis and soil sieving separating, and vermis is collected and is collected simultaneously the earthworm excrement, and young earthworm and earthworm ovum stay in soil, after collecting, soil returns paving, and carries out Soil tillage.
Through above-mentioned 1), 2), 3) namely complete the phase I and repair, the processing time is 1 month.
Second stage, animal, plant and microbial association repair process:
1) animal is repaired: due to the phase I repair finish after, remain with young earthworm and earthworm kind in soil, so the time no longer throw in.Collect once per month during reparation, collection method is with reference to open-air earthworm collecting method, open Yi Tiaogou every two ridges, furrow width 20cm, ditch is long to be decided as the case may be, the vegetable garden black earth of bottom paving one deck 3-5cm, the lemon dregs of paving 6-8cm on black earth (makes the concentration of lemon dregs in laying soil reach 3-5kg/m
2), then above the thick black earth of paving 3-5cm, repave stalk or the straw of 5-10cm, water water after having covered one time, according to circumstances water afterwards, remain that covering is moistening to get final product.Can be gathered into earthworm after feeding intake one day, collect continuously 3 days, collect every day 1 time, after with former earthen backfill.After the second stage reparation finishes, the earthworm vermis all to be collected, collection method adopts natural lighting method (as the phase I).
2) phytoremediation: adopt the mode of interval hedge planting, soaked overnight Bai Sanye seed is drained, be sown in ditch after admixing rhizobium, ditch is apart from 60cm, rear covering one deck thin soil, and transplant dayflower seedling, spacing in the rows 20cm between ditch and ditch; Topdress when Bai Sanye germinates and grow 3-5 sheet true leaf, cradle once in the time of about continued growth to 3 month, Bai Sanye toothing 4cm, dayflower keeps length 15cm; Topdress immediately after cradling.After this stage is repaired end, plant integral body is removed.
3) microorganism is repaired: because soil itself has abundant microorganism, therefore except the rhizobium that are used for the Bai Sanye seed dressing, separately do not add and throw in microorganism, only utilize the activity of earthworm to improve the activity of native country microorganism, accelerate its breeding with movable, improve the root system of plant microenvironment, change the form that exists of heavy metal-polluted soil, be beneficial to the enrichment of plant and animal.Repairing phase finishes microorganism not to be collected.
Through above-mentioned 1), 2), 3) namely complete the second stage reparation, the processing time is 5 months.
Phase III, the same phase I of animal repair process.
The quadravalence section, animal, plant and the same second stage of microbial association repair process; Wherein, during phytoremediation, substitute Bai Sanye and dayflower with rye grass, be specially: adopt the mode of hedge planting ryegrass seed to be sown in the ditch of holding successfully, ditch is apart from 30cm, rear covering one deck thin soil, topdress when rye grass germinates and grow 3-5 sheet leaf, cradle once in the time of about continued growth to 3 month, toothing 6-8cm topdresses after cradling immediately.After this stage is repaired end, plant integral body is removed.
The same phase I of five-stage animal repair process.
The 6th the stage animal, plant and the same second stage of microbial association repair process.
Repairing effect:
The reparation of the present embodiment was sequentially repaired by the first to the 6th stage since the first half of the year, in repair process, animal and plant to the heavy metal-polluted soil enrichment condition see Table 2, table 3.
Table 2 animal heavy metal accumulation situation
Annotate: the second, the data in four, six stages on average obtain by multi-group data
Table 3 plant heavy metal accumulation situation
Annotate: in table, data are the average data that aerial part and under ground portion form through integration according to ratio
In repair process, by the activity of earthworm and the growth of plant, heavy metal in soil is by earthworm and plants enriched, pass through again the collection of earthworm, earthworm excrement and the harvesting of plant, the content of heavy metal in soil is reduced gradually, reach the purpose that thoroughly removes heavy metal in soil, this is a kind of safe, green soil remediation method, it not only can the spoiled soil structure, can also improve soil regime.Each repairing phase soil remediation situation sees Table 4.
The different repairing phase soil remediation of table 4 situation
Through the soil remediation in three cycles, the content of beary metal in this plot has reached predetermined and has repaired target, and soil regime is good, can directly carry out agricultural production.The treatment effect contrast specifically sees Table 5.
Table 5 is repaired plot soil regime (0-20cm)
| Main polluted heavy metals | Cd(mg/kg) | Cu(mg/kg) | Pb(mg/kg) |
| Repair target | ＜0.3 | ＜50 | ＜50 |
| Content before repairing | 0.6-3.6 | 86.2-135.4 | 67.1-154.8 |
| Maximum after repairing | 0.17-0.22 | 29-42 | 33-49 |
Claims (8)
1. the biological renovation method of a heavy-metal contaminated soil, the steps include:
(1) apply earthworm feedstuff in heavy-metal contaminated soil, the mixing of digging is put into vermiculture; Cultivate after 28-35 days, collect the earthworm excrement in upper layer of soil, adopt again the natural lighting method to be gathered into earthworm, young earthworm and earthworm ovum are stayed in soil, contain the cadmium that concentration is 0.6-3.6mg/kg in described heavy-metal contaminated soil, copper and the concentration that concentration is 86.2-135.4mg/kg is the lead of 67.1-154.8mg/kg, described earthworm is large flat No. two earthworms;
(2) adopt the mode planting plants of hedge planting, plant growth cradles its aerial part during to 78-90 days, plant integral body is removed in regrowth 50-70 days;
During planting plants, every 28-35 days, adopt open-air collecting method to be gathered into earthworm and earthworm excrement 1-3 days; After plant integral body removes, adopt the natural lighting method to collect all and become earthworm, young earthworm, and collect earthworm excrement and earthworm ovum;
Wherein, during planting plants, described plant is Bai Sanye (Trifolium repens) and dayflower (Commelina communis), two Plants interval hedge plantings when spring or summer; Described Bai Sanye is with the rear sowing plantation of rhizobium seed dressing;
When autumn or winter planting plant, described plant is rye grass (Lolium perenne).
2. biological renovation method according to claim 1, is characterized in that, in step (1), described earthworm feedstuff is lemon dregs, and the applied amount of lemon dregs is 0.8-1.5kg/m
2
3. biological renovation method according to claim 1, is characterized in that, in step (1), the input density of described large flat No. two earthworms is 0.5-0.6kg/m
2
4. biological renovation method according to claim 1, is characterized in that, in step (1) or (2), described natural lighting method comprises: under natural lighting, soil is successively pushed aside, subsoil is sieved, isolate one-tenth earthworm wherein, soil is returned paving after being gathered into earthworm.
5. biological renovation method according to claim 1, is characterized in that, in step (2), the planting patterns of described dayflower is seedling cultivation; The planting patterns of described rye grass is the sowing plantation.
6. biological renovation method according to claim 1, is characterized in that, in step (2), described Bai Sanye cradles rear toothing 3-5cm, and dayflower cradles rear toothing 12-18cm, and rye grass cradles rear toothing 6-8cm.
7. biological renovation method according to claim 1, is characterized in that, in step (2), described open-air collecting method comprises: containing concentration in heavy-metal contaminated soil edge laying is 3-5kg/m
2The soil of earthworm feedstuff was collected the one-tenth earthworm of laying in soil in 1-2 days afterwards, and young earthworm and earthworm ovum are stayed in soil, afterwards this soil was backfilling in heavy-metal contaminated soil.
8. biological renovation method according to claim 1, is characterized in that, in time waters in vermiculture and planting process, makes the water content of heavy-metal contaminated soil remain on 70-85%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210014161 CN102553904B (en) | 2012-01-17 | 2012-01-17 | Bioremediation method for soil polluted by heavy metals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210014161 CN102553904B (en) | 2012-01-17 | 2012-01-17 | Bioremediation method for soil polluted by heavy metals |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102553904A CN102553904A (en) | 2012-07-11 |
| CN102553904B true CN102553904B (en) | 2013-06-05 |
Family
ID=46401277
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210014161 Active CN102553904B (en) | 2012-01-17 | 2012-01-17 | Bioremediation method for soil polluted by heavy metals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102553904B (en) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102847707B (en) * | 2012-08-31 | 2014-08-06 | 四川农业大学 | Remediation method for heavy metal lead pollution in mine |
| CN103664279A (en) * | 2012-09-05 | 2014-03-26 | 林炳营 | Organic fertilizer for modifying plow layer of low-yield land and repairing heavy metal plants |
| CN103348797A (en) * | 2013-07-29 | 2013-10-16 | 匡仲平 | Method for improving farmland soil |
| CN103521508B (en) * | 2013-09-30 | 2014-12-31 | 中国农业科学院农田灌溉研究所 | Phytoremediation method for soil with cadmium pollution |
| CN103880486B (en) * | 2014-03-14 | 2016-02-24 | 广州城市职业学院 | Microbial-bacterial fertilizer and preparation method thereof and the method for administering heavy-metal contaminated soil |
| CN103962368B (en) * | 2014-04-24 | 2016-04-13 | 常州大学 | A kind of animals and plants work in coordination with the technical method repairing cadmium, lead-contaminated soil |
| CN105268730A (en) * | 2014-05-30 | 2016-01-27 | 上海市环境科学研究院 | Method for restoring heavy metal polluted soil by using earthworm |
| CN105268732A (en) * | 2014-07-07 | 2016-01-27 | 山东乾坤环境工程科技有限公司 | Biological method for repairing contaminated soil |
| CN105642663A (en) * | 2014-10-21 | 2016-06-08 | 周杰 | Flora and fauna comprehensive utilization restoring method of heavy metal contaminated soil |
| CN105290091A (en) * | 2014-10-21 | 2016-02-03 | 苗庆龄 | Method for remedying heavy metal contaminated soil |
| CN104289504B (en) * | 2014-10-21 | 2016-03-30 | 武汉文科生态环境有限公司 | A kind of method for comprehensive repairing of heavy-metal contaminated soil |
| CN104289505B (en) * | 2014-10-21 | 2016-08-24 | 大连地拓重工有限公司 | The method of comprehensive utilization animals and plants restoration of soil polluted by heavy metal |
| CN105710118A (en) * | 2014-10-21 | 2016-06-29 | 周杰 | Method for remediation of heavy metal contaminated soil |
| CN104307856B (en) * | 2014-10-21 | 2016-05-11 | 无锡市太湖湖泊治理有限责任公司 | The animals and plants comprehensive utilization restorative procedure of heavy-metal contaminated soil |
| CN104289508B (en) * | 2014-10-21 | 2016-06-22 | 孙一铭 | The biological renovation method of heavy-metal contaminated soil |
| CN104289509B (en) * | 2014-10-21 | 2016-08-31 | 重庆鼎旺环保园林有限公司 | The method of biological restoration heavy-metal contaminated soil |
| CN104289506B (en) * | 2014-10-21 | 2016-07-06 | 大连地拓重工有限公司 | Cadmium, zinc, lead, copper ion contaminated soil biological renovation method |
| CN105312310B (en) * | 2015-02-04 | 2019-04-26 | 四川农业大学 | A kind of method of biological prosthetic heavy-metal contaminated soil |
| CN105127186A (en) * | 2015-09-30 | 2015-12-09 | 河南行知专利服务有限公司 | Restoration method for heavy metal polluted soil of ore districts |
| CN105127187B (en) * | 2015-09-30 | 2018-06-15 | 福建沃土环保有限公司 | The method that oil-heavy-metal contaminated soil is repaired in a kind of animals and plants-charcoal collaboration |
| CN105170627B (en) * | 2015-10-20 | 2018-08-24 | 南京工业大学 | Method for repairing cadmium-polluted soil by combining microorganisms and plants |
| CN105290094B (en) * | 2015-11-25 | 2017-11-28 | 沈阳大学 | The method polluted using phthalate in Vermicomposting degraded plastic shed soil |
| CN105880278A (en) * | 2016-04-11 | 2016-08-24 | 宇星科技发展(深圳)有限公司 | Animal and plant combined remediation method for heavy metal cadmium contaminated soil |
| CN105945047A (en) * | 2016-07-10 | 2016-09-21 | 中山市绿浪助剂有限公司 | Comprehensive remediation method for soil polluted by heavy metals and application of comprehensive remediation method |
| CN105945043A (en) * | 2016-07-10 | 2016-09-21 | 中山市绿浪助剂有限公司 | Method for rapidly treating heavy metal pollution of soil |
| CN106211845A (en) * | 2016-07-21 | 2016-12-14 | 崇州三宝家庭农场 | A kind of method utilizing Lumbricus and chicken manure to improve soil |
| CN106475406B (en) * | 2016-12-24 | 2019-04-30 | 东莞市碧江源环保科技有限公司 | A kind of ecological restoring method of heavy-metal contaminated soil |
| CN107457254B (en) * | 2017-08-14 | 2020-07-31 | 浙江省农业科学院 | Method and system for efficiently repairing heavy metal contaminated soil by combining plants and animals |
| CN107671113A (en) * | 2017-09-26 | 2018-02-09 | 湖南匡楚科技有限公司 | A kind of method using various plants comprehensive regulation contaminated soil |
| CN107774704A (en) * | 2017-10-09 | 2018-03-09 | 辽宁大学 | A kind of method of heavy metal copper in plant animal microbial association rehabilitating soil |
| CN108651180B (en) * | 2018-05-28 | 2020-03-31 | 浙江农林大学 | Ecological cultivation technology for promoting fast growth of newly-built bamboo forest |
| CN109013676B (en) * | 2018-07-13 | 2020-08-07 | 湖南大学 | Method for promoting heavy metal to be transported to overground part from plant root system |
| CN109365515A (en) * | 2018-09-10 | 2019-02-22 | 王世清 | A kind of administering method of heavy metal pollution of soil |
| CN111185479A (en) * | 2018-12-13 | 2020-05-22 | 四川君和环保股份有限公司 | Microorganism/animal and plant combined ecological restoration method for cadmium pollution of farmland soil |
| CN109550773A (en) * | 2019-01-11 | 2019-04-02 | 青岛冠中生态股份有限公司 | Animals and plants joint granule suitable for heavy-metal contaminated soil is sowed grass seeds by duster restorative procedure |
| CN109530428A (en) * | 2019-01-11 | 2019-03-29 | 青岛冠中生态股份有限公司 | Rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil method |
| CN109590320A (en) * | 2019-01-29 | 2019-04-09 | 广西浩发生物科技有限公司 | The method for repairing the matrix containing heavy metal using earthworm and sugar grass |
| CN110434164A (en) * | 2019-07-08 | 2019-11-12 | 迪天环境技术南京股份有限公司 | One heavy metal species reparation great Ye dandelion high yield method for planting |
| CN110788130A (en) * | 2019-11-18 | 2020-02-14 | 武汉科技大学 | Ecological restoration method for polluted soil in copper ore area |
| CN112642858A (en) * | 2020-12-04 | 2021-04-13 | 张宁 | Soil remediation ecological net and remediation method thereof |
| CN115138672B (en) * | 2022-07-05 | 2023-05-12 | 上海辰山植物园 | Method for repairing copper-polluted soil by combining fast-growing tree seeds and large fungi in layering manner |
| CN115338242B (en) * | 2022-08-16 | 2023-05-30 | 生态环境部南京环境科学研究所 | Method for restoring polluted soil by applying wormcast and probiotics |
| CN115532809A (en) * | 2022-09-27 | 2022-12-30 | 西南科技大学 | Process for treating heavy metal pollution of soil |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101337231A (en) * | 2008-08-12 | 2009-01-07 | 天津师范大学 | Method for promoting lawn absorb heavy metal from consumer garbage compost and increasing photosynthesis amount cooperated with angleworm |
| CN101704015A (en) * | 2009-04-28 | 2010-05-12 | 吴江市土壤肥料技术指导站 | Method for improving phytoremediation efficiency of low-concentration arsenic-contaminated soil |
| CN101797579A (en) * | 2009-12-30 | 2010-08-11 | 中山大学 | Method for treating soil heavy metal combined pollution |
| CN101947539A (en) * | 2010-08-06 | 2011-01-19 | 浙江博世华环保科技有限公司 | Soil remediation method for treating heavy metal pollutants |
| CN101947541A (en) * | 2010-10-14 | 2011-01-19 | 上海第二工业大学 | Method for remediation of soil polluted by polycyclic aromatic hydrocarbons |
-
2012
- 2012-01-17 CN CN 201210014161 patent/CN102553904B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101337231A (en) * | 2008-08-12 | 2009-01-07 | 天津师范大学 | Method for promoting lawn absorb heavy metal from consumer garbage compost and increasing photosynthesis amount cooperated with angleworm |
| CN101704015A (en) * | 2009-04-28 | 2010-05-12 | 吴江市土壤肥料技术指导站 | Method for improving phytoremediation efficiency of low-concentration arsenic-contaminated soil |
| CN101797579A (en) * | 2009-12-30 | 2010-08-11 | 中山大学 | Method for treating soil heavy metal combined pollution |
| CN101947539A (en) * | 2010-08-06 | 2011-01-19 | 浙江博世华环保科技有限公司 | Soil remediation method for treating heavy metal pollutants |
| CN101947541A (en) * | 2010-10-14 | 2011-01-19 | 上海第二工业大学 | Method for remediation of soil polluted by polycyclic aromatic hydrocarbons |
Non-Patent Citations (4)
| Title |
|---|
| Pb~(2+)、Cd~(2+)胁迫作用下蚯蚓、菌根菌及其联合作用对植物修复的影响;杨柳等;《贵州农业科学》;20101130;第38卷(第11期);156-158 * |
| 冯春雨等.重金属污染土壤的生物修复研究现状.《工业安全与环保》.2010,第36卷(第4期),27-28. |
| 杨柳等.Pb~(2+)、Cd~(2+)胁迫作用下蚯蚓、菌根菌及其联合作用对植物修复的影响.《贵州农业科学》.2010,第38卷(第11期),156-158. |
| 重金属污染土壤的生物修复研究现状;冯春雨等;《工业安全与环保》;20100430;第36卷(第4期);27-28 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102553904A (en) | 2012-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102553904B (en) | Bioremediation method for soil polluted by heavy metals | |
| CN103736721B (en) | Method by biological restoration water body with soil and comprehensive utilization of resources | |
| CN104289504B (en) | A kind of method for comprehensive repairing of heavy-metal contaminated soil | |
| CN104289509B (en) | The method of biological restoration heavy-metal contaminated soil | |
| CN108114977A (en) | It is a kind of to utilize super enriching plant and the method in the quick restoring cadmium polluted arable land of energy-source plant crop rotation plantation | |
| CN104285882B (en) | The sheep raising method that second stable breeding of a kind of camellia oleifera lam is partly raised scattered | |
| CN100371093C (en) | Method for repairing soil of cadmium polluted by solanaceae plant | |
| CN102771284B (en) | Ecological cultivation method for houttuynia cordata | |
| CN1926945A (en) | Method for saline-alkali land vegetation recovery by using apocynum root segment for breeding | |
| CN109047308A (en) | A kind of restorative procedure of cadmium pollution soil | |
| CN102513338A (en) | Method for restoring copper-polluted soil by plants | |
| CN105013808A (en) | Method for repairing cadmium-polluted soil through mulberry trees | |
| CN102428818B (en) | Method for preventing exotic weeds from invading by cultivating ramie | |
| CN106258418A (en) | A kind of spring and summer stubble Fructus Capsici rice field-upland field rotation organic cultivation method | |
| CN110352777A (en) | A kind of ecospecies group method of artificial forest land | |
| CN109618863B (en) | Bamboo retaining forest method for improving yield of bamboo shoots in traditional hemp bamboo forest | |
| CN105964682A (en) | Method for restoring soil heavy metal cadmium pollution by use of herba cichorii | |
| CN106258420A (en) | A kind of rice field-upland field rotation organic cultivation method of summer autumn capsicum | |
| CN106305050A (en) | Rotation organic cultivation method for over-summer capsicum annuum L., vegetable vicia faba, early oryza sativa and eleocharis dulcis | |
| CN110508612A (en) | The method of cadmium is remained in a kind of removing farmland | |
| CN110280584A (en) | A kind of heavy metal in soil biology removing method | |
| CN113924930B (en) | Method for replacing and controlling exotic invasive plant mikania micrantha in sloping farmland by utilizing species combination of sweet potatoes and ryegrass | |
| CN106258419A (en) | Autumn stubble Fructus Capsici and Semen Viciae fabae, early rice, Corm Eleocharitis crop rotation organic cultivation method | |
| CN106234000A (en) | Autumn capsicum and Bowl bean, early rice, Corm Eleocharitis, green soyabeans crop rotation organic cultivation method | |
| Vittal et al. | Districtwise promising technologies for rainfed rice based production system in India |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Bioremediation method for soil polluted by heavy metals Effective date of registration: 20140331 Granted publication date: 20130605 Pledgee: Uni-Power Group Co.,Ltd. Pledgor: Zhejiang Bestwa Environmental Protection Science and Technology Co., Ltd. Registration number: 2014990000216 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20160419 Granted publication date: 20130605 Pledgee: Uni-Power Group Co.,Ltd. Pledgor: Zhejiang Bestwa Environmental Protection Science and Technology Co., Ltd. Registration number: 2014990000216 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model |