CN109530428A - Rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil method - Google Patents
Rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil method Download PDFInfo
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- CN109530428A CN109530428A CN201910027111.4A CN201910027111A CN109530428A CN 109530428 A CN109530428 A CN 109530428A CN 201910027111 A CN201910027111 A CN 201910027111A CN 109530428 A CN109530428 A CN 109530428A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
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Abstract
The present invention provides rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil method, it is related to heavy metal technical field of soil remediation, by the root nodule for preparing leguminous plant using cracking process, ready root nodule is smashed to pieces before carrying out phytoremediation, it dresses seed with target rehabilitation plant, utilizes granule spray seeding technique sowing leguminous plant and nutrient matrix layer in heavy-metal contaminated soil region.The present invention has the yield for improving leguminous plant, improves phytoremediation heavy metal efficiency, and the later period does not have to lift-augmented mechanism, reduces the destruction to soil texture, promotes the beneficial effect of soil texture quality.
Description
Technical field
The present invention relates to heavy metal technical field of soil remediation, specifically rhizobium-plant symbiosis system remediating heavy metal
The method of cadmium pollution soil.
Background technique
With the development of industrial or agricultural, a large amount of pollutant is entered in soil environment, and soil environment is worsening, wherein weighing
The problem of metallic pollution, is especially prominent.Heavy metal poor mobility in the soil, residence time are long, cannot be dropped by edaphon
Solution, it can indirectly be entered into the human body by " soil-plant/water-human body ", be absorbed by the body, seriously threaten the mankind's
Life and health.Heavy metal pollution of soil has seriously affected human health and the ecosystem is stablized, it has also become urgently to be resolved shows
Real problem.
The technical staff in current many fields has done many researchs for heavy metal recovery technique, at present generally by solid
Change effect, activation extraction two ways remove the heavy metal pollution in soil.Solidification mainly utilizes the methods of physics, chemistry
The state for carrying out heavy metal in stable soil makes it be not easy to be reduced its recycling property between biology by bio-absorbable, and drops
The toxicity of low heavy metal in soil, curing method regulation effect is good, it is short to last, and it is very high that there are costs, it is possible to destroy soil original
The problem of physicochemical property come.Curing method is only applicable to the improvement of small-scale heavy metal in soil pollutant.Activation extraction
It is to be mentioned the heavy metal in soil using the plant that can adsorb heavy metal contaminants by the heavy metal in biologically rehabilitating soil
It takes out, achievees the purpose that reduce heavy metal in soil concentration.Although existing using phytoremediation and administering period length, plant life
The small defect of object amount, but since it has many advantages, such as that influence at low cost, to environment is small, convenient to carry out, have become ring now
Most promising a kind of restorative procedure in the reparation field of border.
Largely studies have shown that using the heavy metal pollution in vegetation repair soil, the nitrogen content on heavy metal pollution ground
Extreme deficiency be one of revegetation main limiting factor.Rhizobium-legume symbiosis system is that nitrogen fixing capacity is strongest
Biological nitrogen fixation system plays a significant role in promoting heavy metal pollution nitrogen cycle and Nutrient Accumulation.
The present invention provides a kind of method of rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil, mainly passes through
The root nodule that leguminous plant is prepared using cracking process is smashed to pieces ready root nodule before carrying out phytoremediation, with target reparation
Plant seed dressing sows leguminous plant using granule spray seeding technique in heavy-metal contaminated soil region, utilizes the root nodule of leguminous plant
Bacterium endlessly can provide nutrition by inside plants, while rhizobium can secrete improving plant growth substance, promote plant raw
Long and Heavy Metal Accumulation achievees the purpose that heavy metal contaminants in rehabilitating soil with this.
Summary of the invention
The present invention provides a kind of method of rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil, solves existing
When having in technology using rhizobium, rhizobium need to be cultivated, the cumbersome technical problem of enriching plant curing step.
The present invention is implemented as follows: steps are as follows:
(1) root nodule of leguminous plant is prepared using cracking process；
(2) before sowing, the root nodule of step (1) is taken, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, carried out
Seeds of leguminous plant seed dressing to be sowed；
(3) it is constructed using the method that granule is sowed grass seeds by duster, includes needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
The nutrient matrix layer of seed, with a thickness of 5~10mm；
The nutrient matrix layer includes the component of following volumes proportion: loam 15-29%, dewatered sludge 8-20%, farming
Object stalk 13-18%, ferrous sulfate 8-15%, sepiolite 2-4%, ground phosphate rock 1-3%, granule agent 0.35-0.38%, plant species
Remaining all components in addition to vegetable seeds are placed in hydroseeder by sub- 0.5-5%, organic compound fertilizer 2-4%, clear water 23-38%
In, it stirs, is proportionally added into after step (2) mixes the vegetable seeds of bacterium and sows grass seeds by duster；
(4) according to the growing state of plant, the aerial part of plant is gathered in after sowing grass seeds by duster 3-5 months.
Agricultural crop straw is crushing maize stalk, improvement soil texture, heat preservation, improve reservoir storage, while rich in nitrogen, phosphorus,
Potassium, calcium, magnesium and organic matter, can be decomposed by the microorganisms, and provide nutrient for plant growth.
Embodiment as one preferred, the nutrient matrix layer that step (3) is chosen include the component of following volumes proportion:
Loam 17%, dewatered sludge 20%, agricultural crop straw 16.5%, ferrous sulfate 10.15%, sepiolite 3%, ground phosphate rock 2.5%,
Granule agent 0.35%, vegetable seeds 4.5%, organic compound fertilizer 3%, clear water 23%.
Embodiment as one preferred, the nutrient matrix layer cohesive strength>37kPa, isolation degree<15%, organic matter
Content > 18%.
Embodiment as one preferred, the agricultural crop straw are crushing maize stalk, and the organic compound fertilizer is
Chicken manure, the animal wastes such as pig manure.
Embodiment as one preferred, the vegetable seeds are silk tree, gleditsia sinensis, and white clover is any one in soybean
Kind.
Embodiment as one preferred, per acre with 5~10 plants of root nodules in step (2).
Embodiment as one preferred, step (1) is the following steps are included: plant silk tree, gleditsia sinensis, white vehicle in experimental field
Axis grass and soybean Four Plants, early period applies a small amount of urea, when plant growth 75~83 days, chooses in Four Plants be good for respectively
Strong plant is simultaneously dug out, and root soil is cleaned, the plant for therefrom selecting root Nodule Growth relatively good, the biggish root nodule of clip,
It is cut in clip together with together with the part root that root nodule connects, the root length cut is 0.6cm, the root nodule that will be cut
It is placed in ventilating and cooling place and dries in the shade to be placed under drying condition and save；
Embodiment as one preferred, 3kg/ mus of urea amount of application in step (1).
Beneficial effects of the present invention: the application directly by rhizobium made from cracking process smash to pieces plus cold water processing after, directly
It connects and dresses seed with target plant seed, method is simple, while ensure that the activity of rhizobium.Utilize the beans with nitrogen fixation
Section plant carries out heavy-metal contaminated soil reparation, and the later period does not need to increase the application of nitrogenous fertilizer, utilizes leguminous plant and rhizobium shape
At Symbiotic association can reduce the investment of nitrogenous fertilizer, increase soil with organic matter content, improve ecological environment of soil.This Shen
It middle please be dressed seed using rhizobium, the yield of leguminous plant can be improved, promote the growth of leguminous plant, improve phytoremediation
The efficiency of heavy metal reduces the destruction to soil texture, and after carrying out phytoremediation, soil texture quality has a certain upgrade.
Specific embodiment
Technical solution of the present invention is carried out below in conjunction with specific embodiments of the present invention clear, be fully described by, shown
So, described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The scope of protection of the invention.
Rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil method, steps are as follows:
(1) root nodule of leguminous plant is prepared using cracking process；
(2) before sowing, the root nodule of step (1) is taken, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, carried out
Seeds of leguminous plant seed dressing to be sowed；
(3) it is constructed using the method that granule is sowed grass seeds by duster, includes needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
The nutrient matrix layer of seed, with a thickness of 5~10mm；
The nutrient matrix layer includes the component of following volumes proportion: loam 15-29%, dewatered sludge 8-20%, farming
Object stalk 13-18%, ferrous sulfate 8-15%, sepiolite 2-4%, ground phosphate rock 1-3%, granule agent 0.35-0.38%, plant species
Remaining all components in addition to vegetable seeds are placed in hydroseeder by sub- 0.5-5%, organic compound fertilizer 2-4%, clear water 23-38%
In, it stirs, is proportionally added into after step (2) mixes the vegetable seeds of bacterium and sows grass seeds by duster；
(4) aerial part of plant is gathered in after growing 3-5 months according to the growing state of plant.
Further, the nutrient matrix layer that step (3) is chosen includes the component of following volumes proportion: loam 17%, desiccation
Sludge 20%, agricultural crop straw 16.5%, ferrous sulfate 10.15%, sepiolite 3%, ground phosphate rock 2.5%, granule agent 0.35%,
Vegetable seeds 4.5%, organic compound fertilizer 3%, clear water 23%.
Further, the nutrient matrix layer cohesive strength>37kPa, isolation degree<15%, the content of organic matter>18%.
Further, the agricultural crop straw is crushing maize stalk, and the organic compound fertilizer is chicken manure, the domestic animals such as pig manure
Excrement.
Further, the vegetable seeds is silk tree, gleditsia sinensis, white clover, any one in soybean.
Further, per acre with 5~10 plants of root nodules in step (2).
Further, step (1) is the following steps are included: plant silk tree, gleditsia sinensis, white clover and four kinds of soybean in experimental field
Plant, early period applies a small amount of urea, when plant growth 75~83 days, chooses healthy and strong plant in Four Plants respectively and and digs
Out, clean root soil, therefrom select the relatively good plant of root Nodule Growth, the biggish root nodule of clip, in clip together with
It is cut together with the part root of root nodule connection, the root length cut is 0.6cm, avoids the connecting portion of root nodule and root dirty
Dye.It the root nodule cut is placed in ventilating and cooling place dries in the shade to be placed under drying condition and save；
Further, 3kg/ mus of urea amount of application in step (1).
Embodiment 1
(1) silk tree, gleditsia sinensis, white clover and soybean Four Plants are planted in experimental field, applies a small amount of urea, urea early period
Amount of application is 3kg/ mus, guarantees that seedling has enough nitrogen nutrition before nodule formation, when plant growth 75~83 days, respectively
More healthy and strong plant is grown in selection Four Plants, plant is dug out, cleans the soil of root, is chosen in four strains respectively
The relatively good plant of root Nodule Growth, the biggish root nodule of clip, in clip together with together with the part root that root nodule connects
It cuts, the root length cut is 0.6cm, and the connecting portion of root nodule and root is avoided to pollute.The root nodule cut is placed in ventilation yin
Liang Chu dries in the shade, and saves under drying condition.
(2) when sowing, root nodule is cut off with knife, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, can be mixed
Kind.Per acre with 8 plants of root nodules in the present embodiment.
(3) it is taken for the nutrient matrix layer of heavy metal reparation by volume percentage: loam 17%, dewatered sludge 20%,
Agricultural crop straw 16.5%, ferrous sulfate 10.15%, sepiolite 3%, ground phosphate rock 2.5%, granule agent 0.35%, vegetable seeds
4.5%, remaining all components in addition to vegetable seeds are placed in hydroseeder by organic fertilizer 3%, clear water 23%, are sufficiently stirred
It is even, it is proportionally added into after step (2) mixes the vegetable seeds of bacterium and sows grass seeds by duster in the heavy metal pollution region that needs are repaired.The Nutrient medium
Matter layer cohesive strength>37kPa, isolation degree<15%, the content of organic matter>18%.Agricultural crop straw is crushing maize stalk, conditioned soil
Earth structure, heat preservation improves reservoir storage, while being rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter, can be decomposed by the microorganisms, raw for plant
It is long that nutrient is provided.Organic compound fertilizer is mainly chicken manure, the animal wastes such as pig manure.Vegetable seeds is silk tree, gleditsia sinensis, white clover,
One or more of soybean.
It is constructed using the method that granule is sowed grass seeds by duster, includes seed needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
Nutrient matrix layer, the present embodiment sows grass seeds by duster with a thickness of 8mm.According to the growing state of plant, to plant after growing 3-5 months
Aerial part is gathered in.
Embodiment 2
(1) silk tree, gleditsia sinensis, white clover and soybean Four Plants are planted in experimental field, applies a small amount of urea, urea early period
Amount of application is 3kg/ mus, guarantees that seedling has enough nitrogen nutrition before nodule formation, when plant growth 75~83 days, selection
More healthy and strong plant is grown in Four Plants, plant is dug out, cleans the soil of root, chooses root in four strains respectively
The relatively good plant of Nodule Growth, the biggish root nodule of clip, cuts in clip together with together with the part root that root nodule connects,
The root length cut is 0.6cm, and the connecting portion of root nodule and root is avoided to pollute.The root nodule cut is placed in ventilating and cooling place yin
It is dry, it is saved under drying condition.
(2) when sowing, root nodule is cut off with knife, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, for mixing
Kind.Per acre with 10 plants of root nodules in the present embodiment.In the present embodiment, vegetable seeds is silk tree, gleditsia sinensis, white clover, in soybean
One or more, mixed after the seed wait sow is dressed seed respectively.
(3) it is taken for the nutrient matrix layer of heavy metal reparation by volume percentage: loam 15%, dewatered sludge 8%,
Agricultural crop straw 18%, ferrous sulfate 15%, sepiolite 2%, ground phosphate rock 1%, granule agent 0.35%, vegetable seeds 5% are organic
Fertilizer 4%, remaining all components in addition to vegetable seeds are placed in hydroseeder, stir by clear water 31.65%, according to
Ratio is added after step (2) mix the vegetable seeds of bacterium and sows grass seeds by duster in the heavy metal pollution region that needs are repaired.The nutrient matrix layer
Cohesive strength>37kPa, isolation degree<15%, the content of organic matter>18%.Agricultural crop straw is crushing maize stalk, improves soil knot
Structure, heat preservation improve reservoir storage, while being rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter, can be decomposed by the microorganisms, mention for plant growth
For nutrient.Organic compound fertilizer is mainly chicken manure, the animal wastes such as pig manure.
It is constructed using the method that granule is sowed grass seeds by duster, includes seed needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
Nutrient matrix layer, the present embodiment sows grass seeds by duster with a thickness of 10mm.According to the growing state of plant, the 3-5 months grounds to plant
It is gathered in part.
Embodiment 3
(1) silk tree, gleditsia sinensis, white clover and soybean Four Plants are planted in experimental field, applies a small amount of urea, urea early period
Amount of application is 3kg/ mus, guarantees that seedling has enough nitrogen nutrition before nodule formation, when plant growth 75~83 days, selection
The more healthy and strong plant of Four Plants growth, plant is dug out, the soil of root is cleaned, selects root root in Four Plants respectively
The preferable plant of tumor growth fraction, the biggish root nodule of clip, cuts together with together with the part root that root nodule connects in clip, cuts
Under root length be 0.6cm, avoid the connecting portion of root nodule and root pollute.The root nodule cut is placed in ventilating and cooling place yin
It is dry, it is saved under drying condition.
(2) when sowing, root nodule is cut off with knife, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, for mixing
Kind.Per acre with 5 plants of root nodules in the present embodiment.In the present embodiment, vegetable seeds is silk tree, gleditsia sinensis, white clover, in soybean
One or more mix after the vegetable seeds wait sow is dressed seed respectively.
(3) it is taken for the nutrient matrix layer of heavy metal reparation by volume percentage: for loam 29%, dewatered sludge
14.12%, agricultural crop straw 13%, ferrous sulfate 8%, sepiolite 4%, ground phosphate rock 3%, granule agent 0.38%, vegetable seeds
0.5%, remaining all components material other than vegetable seeds is placed in hydroseeder, adds by organic fertilizer 2%, clear water 26%
Water stirs, and step (2) are proportionally added and mix the heavy metal pollution repaired in needs of sowing grass seeds by duster after the vegetable seeds of bacterium
Region.The nutrient matrix layer cohesive strength>37kPa, isolation degree<15%, the content of organic matter>18%.Agricultural crop straw is to crush
Corn stover improves soil texture, and heat preservation improves reservoir storage, while being rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter, can be by micro- life
Object decomposes, and provides nutrient for plant growth.Organic compound fertilizer is mainly chicken manure, the animal wastes such as pig manure.
It is constructed using the method that granule is sowed grass seeds by duster, includes seed needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
Nutrient matrix layer, the present embodiment sows grass seeds by duster with a thickness of 5mm.According to the growing state of plant, the 3-5 months overground parts to plant
Divide and is gathered in.
Embodiment 4
(1) silk tree, gleditsia sinensis, white clover and soybean Four Plants are planted in experimental field, applies a small amount of urea, urea early period
Amount of application is 3kg/ mus, guarantees that seedling has enough nitrogen nutrition before nodule formation, when plant growth 75~83 days, selection
The more healthy and strong plant of Four Plants growth, plant is dug out, the soil of root is cleaned, selects root root in Four Plants respectively
The preferable plant of tumor growth fraction, the biggish root nodule of clip, cuts together with together with the part root that root nodule connects in clip, cuts
Under root length be 0.6cm, avoid the connecting portion of root nodule and root pollute.The root nodule cut is placed in ventilating and cooling place yin
It is dry, it is saved under drying condition.
(2) when sowing, root nodule is cut off with knife, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, for mixing
Kind.Per acre with 5 plants of root nodules in the present embodiment.In the present embodiment, vegetable seeds is silk tree, gleditsia sinensis, white clover, in soybean
One or more mix after the vegetable seeds wait sow is dressed seed respectively.
(3) it is taken for the nutrient matrix layer of heavy metal reparation by volume percentage: for loam 15.5%, dewatered sludge
16.7%, agricultural crop straw 13.5%, ferrous sulfate 9.5%, sepiolite 2.5%, ground phosphate rock 1.2%, granule agent 0.36%, plant
Remaining all components material other than vegetable seeds is placed in spray by species 0.7%, organic fertilizer 2.04%, clear water 38%
It broadcasts in machine, water is added to stir, step (2) are proportionally added and mix the weight repaired in needs of sowing grass seeds by duster after the vegetable seeds of bacterium
Metallic pollution region.The nutrient matrix layer cohesive strength>37kPa, isolation degree<15%, the content of organic matter>18%.Crops straw
Stalk is crushing maize stalk, improves soil texture, and heat preservation improves reservoir storage, while being rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter,
It can be decomposed by the microorganisms, provide nutrient for plant growth.Organic compound fertilizer is mainly chicken manure, the animal wastes such as pig manure.
It is constructed using the method that granule is sowed grass seeds by duster, includes seed needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
Nutrient matrix layer, the present embodiment sows grass seeds by duster with a thickness of 5mm.According to the growing state of plant, the 3-5 months overground parts to plant
Divide and is gathered in.
Experimental example 1
With silk tree, gleditsia sinensis, white clover, soybean Four Plants are experimental subjects, and rhizobium are being added and root is not added respectively
When tumor bacterium, the comparison of remediating heavy metal cadmium pollution soil is carried out.
Experimental method: by with Cd (Ac)2The form of 2H2O increases a certain amount of heavy metal cadmium into soil, artificial to manufacture
Four groups, every group 2 pieces, amount to the identical heavy metal cadmium soil of eight pieces of areas, it is identical to organize interior soil cadmium concentration.Every group is planted respectively
Silk tree is planted, the plant variety of gleditsia sinensis, white clover, one of soybean plant, every group of plantation is not overlapped.One block of soil is pressed in group
Repaired according to the method for embodiment 1, as experimental group, another block of soil according to 1 method of embodiment and remove seed dressing step into
Row is repaired, as a comparison group.
In each group soil the concentration of cadmium be respectively 142.25mg/kg, 138.45mg/kg, 141.74mg/kg,
139.64mg/kg after sowing grass seeds by duster 4 months, surveys the heavy metal cadmium content of experimental group, control group plants, the results are shown in Table 1.
Table 1 is dressed seed to be compared with the reparation that do not dress seed
As it can be seen from table 1 plant has adsorbed more heavy metal elements after rhizobium are added.The silk tree of seed dressing, soap
Kindred plant that angle, white clover, soybean are not dressed seed absorption heavy metal has been respectively increased 27.62%, 23.58%,
17.90%, 38.62%.
Field experiment
Certain heavy metal pollution place is chosen, is found after being detected to the content of beary metal in collected soil, is repaired
Cadmium content is 47.65~148.53mg/kg, average value 105.84mg/kg in regional soil.With embodiment 1 method into
Capable+phytoremediation of sowing grass seeds by duster.
Before being repaired, the soil of three pieces of different zones is acquired labeled as soil 1, soil 2, soil 3, progress heavy metal cadmium
The detection of content, as a result as shown in the table, soil 1, soil 2,3 heavy metal cadmium concentration of soil are respectively 105.84mg/
Kg, 89.56mg/kg, 134.63mg/kg carry out GPS positioning, using 1 restorative procedure of embodiment, mixed planting silk tree, gleditsia sinensis,
White clover and soybean carry out soil remediation, after sowing grass seeds by duster 4 months, acquire sample in above-mentioned three pieces of regions, each region chooses 4
Point carries out cadmium content detection, the results are shown in Table 2.
2 Four Plants heavy metal adsorption effect of table
As can be seen from Table 2, cadmium content is 105.84ng/kg before soil 1 is repaired, average content is 64.01mg/kg after reparation,
Cadmium content decline 39.53%；Cadmium content is 89.56mg/kg, cadmium content average out to 56.60mg/ after reparation before soil 2 is repaired
Kg, cadmium content decline 36.80%；Cadmium content is 134.63mg/kg before soil 3 is repaired, and be cadmium content after reparation is 86.29mg/
Kg, cadmium content decline 35.90%.It can be seen that the content of the soil cadmium metal of 4 points in three pieces of regions decreases.It sows grass seeds by duster 6
After month, plant growth is healthy and strong.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, made any modification, equivalent replacement, improve etc., it should all be included in the protection scope of the present invention.
Claims (8)
1. rhizobium-plant symbiosis system remediating heavy metal cadmium pollution soil method, which is characterized in that steps are as follows:
(1) root nodule of leguminous plant is prepared using cracking process；
(2) before sowing, the root nodule of step (1) is taken, is placed in porcelain jar and smashs to pieces, in addition a little cold water stirs evenly, is carried out wait broadcast
The seeds of leguminous plant seed dressing of kind；
(3) it is constructed using the method that granule is sowed grass seeds by duster, includes seed needing the heavy metal pollution region repaired to sow grass seeds by duster one layer
Nutrient matrix layer, with a thickness of 5~10mm；
The nutrient matrix layer includes the component of following volumes proportion: loam 15-29%, dewatered sludge 8-20%, crops straw
Stalk 13-18%, ferrous sulfate 8-15%, sepiolite 2-4%, ground phosphate rock 1-3%, granule agent 0.35-0.38%, vegetable seeds
Remaining all components in addition to vegetable seeds are placed in hydroseeder by 0.5-5%, organic compound fertilizer 2-4%, clear water 23-38%,
It stirs, is proportionally added into after step (2) mixes the vegetable seeds of bacterium and sows grass seeds by duster；
(4) aerial part of plant is gathered in after plant strain growth 3-5 months according to the growing state of plant.
2. rhizobium according to claim 1-plant symbiosis system remediating heavy metal cadmium pollution soil method, feature
It is, the nutrient matrix layer that step (3) is chosen includes the component of following volumes proportion: loam 17%, dewatered sludge 20%, farming
Object stalk 16.5%, ferrous sulfate 10.15%, sepiolite 3%, ground phosphate rock 2.5%, granule agent 0.35%, vegetable seeds
4.5%, organic compound fertilizer 3%, clear water 23%.
3. rhizobium according to claim 2-plant symbiosis system remediating heavy metal cadmium pollution soil method, feature
It is, the nutrient matrix layer cohesive strength>37kPa, isolation degree<15%, the content of organic matter>18%.
4. rhizobium according to claim 3-plant symbiosis system remediating heavy metal cadmium pollution soil method, feature
It is, the agricultural crop straw is crushing maize stalk, and the organic compound fertilizer is chicken manure, pig manure, sheep dung.
5. rhizobium according to claim 2 or 3-plant symbiosis system remediating heavy metal cadmium pollution soil method,
It is characterized in that, the vegetable seeds is silk tree, gleditsia sinensis, white clover, any one in soybean.
6. rhizobium according to claim 5-plant symbiosis system remediating heavy metal cadmium pollution soil method, feature
It is, per acre with 5~10 plants of root nodules in step (2).
7. rhizobium according to claim 6-plant symbiosis system remediating heavy metal cadmium pollution soil method, feature
It is, step (1) is the following steps are included: plant silk tree in experimental field, gleditsia sinensis is applied white clover and soybean Four Plants, early period
Add a small amount of urea, when plant growth 75~83 days, chooses healthy and strong plant in Four Plants respectively and and dig out, clean root mud
Soil, the plant for therefrom selecting root Nodule Growth relatively good, the biggish root nodule of clip, in clip together with the portion being connect with root nodule
Point root is cut together, and the root length cut is 0.6cm, the root nodule cut is placed in ventilating and cooling place dries in the shade and be placed on drying
Under the conditions of save.
8. rhizobium according to claim 7-plant symbiosis system remediating heavy metal cadmium pollution soil method, feature
It is, 3kg/ mus of urea amount of application in step (1).
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| CN114958821A (en) * | 2022-06-15 | 2022-08-30 | 中化地质矿山总局地质研究院 | Immobilized SRB pellet, ecological particle, preparation method and application thereof |
| CN114958821B (en) * | 2022-06-15 | 2024-04-26 | 中化地质矿山总局地质研究院 | Immobilized SRB (SRB) pellets, ecological granules and preparation method and application thereof |
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