CN105268730A - Method for restoring heavy metal polluted soil by using earthworm - Google Patents
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Abstract
The present invention discloses a method for restoring heavy metal polluted soil by using earthworm. The method comprises: adopting earthworm as an organism subject; carrying out greenhouse covering on polluted soil to be restored with a simple single-building single-layer film; paving an earthworm culture medium with a certain thickness on the polluted soil; carrying out plowing uniform-mixing on the culture medium and the heavy metal polluted soil to form an earthworm living layer with a certain thickness, and pouring earthworms having a proper number in the region; controlling the earthworm culture conditions in the greenhouse; after restoring for 3 months, paving a culture medium with a certain thickness on the surface layer, and continuously restoring for 3 months; separating the earthworms enriching the heavy metal from the soil; detecting the concentration of the heavy metal in the restored soil; and if the concentration of the heavy metal in the restored soil can not meet the restoring requirement, continuously restoring by adopting 3 months as the period. According to the present invention, various parameters during the restoring process are quantified, the heavy metal polluted soil restoring efficiency is high, the operation is simple and easy to perform, the time-consuming short, and the project implementation is easily achieved.
Description
Technical field
The present invention relates to a kind of method utilizing earthworm restoration of soil polluted by heavy metal, particularly relate to and utilize the accumulation ability of heavy metal in Soil of Earthworms heavy metal accumulation in earthworm body, can be applicable to the reparation of heavy metal combined contamination soil.
Background technology
Soil is the important natural environment that the mankind depend on for existence, and along with the fast development of industry, the heavy metal pollution problem of soil becomes increasingly conspicuous, and the combined pollution that coexists of a few heavy metal species often, and soil pollution reparation comes into one's own day by day.Research uses efficiently, the reparation of the geobiont recovery technique heavy metal contaminated soil of low cost has important progradation.The current recovery technique about heavy metal-polluted soil reports physics or the chemical method of mainly heavy metal stable curing, concerns about bio restorative procedure report is less, in addition, Chen Tongbin reports the heavy metal utilized in plants enriched soil, solve the reparation to heavy metal polluted soil in mine field, there is certain environment, economic and social profit.But the drawbacks such as it is low to there is remediation efficiency, length consuming time, the present invention uses the heavy metal in earthworm bioaccumulation soil, has quantized the parameters in repair process, is convenient to the enforcement carrying out engineering.The remediation efficiency of heavy metal contaminated soil is high, easy to operation, consuming time short, is convenient to implement.
Summary of the invention
Technical scheme of the present invention is as follows:
A kind of method of earthworm restoration of soil polluted by heavy metal that utilizes is as follows: using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as biological subject; Contaminated soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse span: 6-8 rice, shoulder height: 1.5-2 rice, rise 2.5-3.5 rice, arch spacing 0.6-1 rice, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, top covers black external sunshade cloth, facilitates cooling in summer.Film is determined by zinc-plated draw-in groove and wire clamping, and greenhouse material all adopts galvanizing to soak; Measure the heavy metal concentration in contaminated soil; Contaminated soil is laid the earthworm culture medium of 20 cm thicks; Mixed by 25 centimetres, the top layer Soil tillage of culture medium and heavy-metal contaminated soil, form the earthworm life layer of 45 cm thicks, the repair action that Earthworm on Heavy Metal in Soil pollutes mainly carries out in this region; Every square metre is placed earthworm is 10,000-3 ten thousand, and the body weight of earthworm is 300-500 milligram, for there being the healthy earthworm of reproduction endless belt; Keep the cultivation temperature 15 ~ 28 DEG C of earthworm in booth, in earthworm life layer, moisture accounts for 55-75% (weight), pH5.5 ~ 8, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth; Repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, under aforementioned cultivating condition, continue reparation 3 months; Use light source to drive earthworm, be stripped to the life layer of earthworm gradually by top layer, the earthworm being enriched heavy metal is separated with soil; Detect the concentration of heavy metal in soil after repairing; If the concentration of heavy metal in soil does not still reach the requirement of reparation, to continue under aforementioned cultivating condition with 3 months as the cycle carries out reparation.
Preferred version, a kind of method with earthworm restoration of soil polluted by heavy metal, specifically implement as follows:
(1) the choosing of test organism (earthworm)
Using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; Heavy metal contaminated soil carries out the enrichment experiment of heavy metal;
(2) contaminated soil environmental control equipment
Soil simple type single the monofilm greenhouse needing to repair is covered, and this product is a kind of simple type single monofilm greenhouse.This greenhouse has the features such as structure is simple, easy for installation, economical and practical, belong to economical greenhouse, greenhouse span: 6-8 rice, shoulder height: 1.5-2 rice, rises 2.5-3.5 rice, arch spacing 0.6-1 rice, domestic 0.10 millimeters thick plastic sheeting, arrange in both sides and manually roll up film, ventilation equipment, top covers black external sunshade cloth, facilitates cooling in summer.Film is determined by zinc-plated draw-in groove and wire clamping, and greenhouse material all adopts galvanizing to soak, both artistic and practical, corrosion resistant.Practical, service life can reach more than 10 years.Greenhouse size: 30 meters × 6 meters × 2.5 meters; Two, 30 meters × 6 meters × 3 meters; Three, 32 meters × 8 meters × 3.35 meters.
(3) heavy metal in soil Concentration Testing before repairing
Before reparation, detect the concentration of Heavy Metals in Contaminated Soils.
(4) earthworm life layer is set
The soil of heavy metal pollution is laid the earthworm culture medium of 20 cm thicks, culture medium optimum formula is: fresh cow dung 75-85%, agricultural residues powder 15-25%, beneficial organism active bacteria liquid EM bacterium liquid (1: 200), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, the repair action of Soil of Earthworms arable layer heavy metal pollution mainly carries out in this region; Every square metre is placed earthworm is 10,000-3 ten thousand, and the body weight of earthworm is 300-500 milligram, for there being the healthy earthworm of reproduction endless belt.
(5) repair process earthworm condition of culture is arranged
Keep the cultivation temperature 15 ~ 28 DEG C of earthworm, in earthworm life layer, moisture accounts for 55-75% (weight), pH5.5 ~ 8, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
(6) repairing efficiency is arranged
Repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
(7) heavy metal in soil Concentration Testing after repairing
Use light source to drive earthworm, be stripped to the life layer of earthworm gradually by top layer, the earthworm being enriched heavy metal is separated with the soil after reparation; Detect the concentration of heavy metal in soil after repairing, if the concentration of heavy metal in soil does not still reach the requirement of reparation, to continue under aforementioned cultivating condition with 3 months as one-period reparation.
Beneficial effect of the present invention:
A kind of method with earthworm restoration of soil polluted by heavy metal of the present invention, it is a kind of soil remediation method set up based on the bioconcentration of earthworm heavy metal, there is rehabilitation expense low, reparation required time is short, cost performance is high, workable, practical, be convenient to carry out the advantages such as repair process regulation and control; The method overcomes the deficiency of existing contaminated soil bioremediation technology, experimental result objective reality, can complement one another with microorganism or plant restoration method.
Accompanying drawing explanation
Fig. 1 is the greenhouse outside drawing of embodiment 1 earthworm repairing polluted soil;
Fig. 2 is the inside greenhouse figure of embodiment 1 earthworm repairing polluted soil;
Fig. 3 is that embodiment 1 utilizes earthworm repairing polluted soil procedure chart;
Fig. 4 is the condition diagram after embodiment 1 utilizes earthworm repairing polluted soil.
Detailed description of the invention
Below in conjunction with specific embodiment and accompanying drawing, the present invention is further detailed explanation.These embodiments are implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention are not limited to following embodiment.
The method of embodiment 1 earthworm cadmium pollution soil repair
One, test procedure:
1, using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; Cadmium pollution soil is carried out to the enrichment experiment of heavy metal;
2, soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse size: 30 meters × 6 meters × 3 meters, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, and top covers black external sunshade cloth, facilitates cooling in summer.
3, in the greenhouse of 30 meters × 6 meters × 3 meters, choose 3 pieces, the plot of 5 meters × 2 meters, the concentration of setting Cadium In Polluted Soil is respectively 125mg/kg, 25mg/kg, 5mg/kg.
4, on the soil of cadmium pollution, lay the earthworm culture medium of 20 cm thicks, culture medium prescription is: fresh cow dung 80%, agricultural residues powder 20%, beneficial organism active bacteria liquid EM bacterium liquid (being 1: 200 by weight proportion), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, every square metre is placed earthworm is 20,000, and the body weight of earthworm is 350 milligrams, for there being the healthy earthworm of reproduction endless belt.
5, the cultivation temperature 23 DEG C of earthworm is kept, in earthworm life layer, moisture accounts for 70% (weight), pH7.1, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
6, repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
7, use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal cadmium is separated with the soil after reparation;
Two, result of the test:
Repair after 6 months, detect the concentration of Cadmium in Soil, the concentration of Cadmium in Soil is respectively 113.37mg/kg, 13.17mg/kg, 0.26mg/kg.
Embodiment 2 earthworm repairs the method for mercury contaminated soil
One, test procedure:
1, using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; Mercury contaminated soil is carried out to the enrichment experiment of heavy metal;
2, soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse size: 30 meters × 6 meters × 3 meters, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, and top covers black external sunshade cloth, facilitates cooling in summer.
3, in the greenhouse of 30 meters × 6 meters × 3 meters, choose 3 pieces, the plot of 5 meters × 2 meters, in setting contaminated soil, the concentration of mercury is respectively 125mg/kg, 25mg/kg, 5mg/kg.
4, on the soil of mercury pollution, lay the earthworm culture medium of 20 cm thicks, culture medium prescription is: fresh cow dung 80%, agricultural residues powder 20%, beneficial organism active bacteria liquid EM bacterium liquid (being 1: 200 by weight proportion), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, every square metre is placed earthworm is 20,000, and the body weight of earthworm is 350 milligrams, for there being the healthy earthworm of reproduction endless belt.
5, the cultivation temperature 23 DEG C of earthworm is kept, in earthworm life layer, moisture accounts for 70% (weight), pH7.1, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
6, repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
7, use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal Hg is separated with the soil after reparation;
Two, result of the test:
Repair after 6 months, detect the concentration of mercury in soils, the concentration of mercury in soils is respectively 123.45mg/kg, 13.27mg/kg, 0.94mg/kg.
Embodiment 3 earthworm repairs the method for As polluted soil
One, test procedure:
1, using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; As polluted soil is carried out to the enrichment experiment of heavy metal;
2, soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse size: 30 meters × 6 meters × 3 meters, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, and top covers black external sunshade cloth, facilitates cooling in summer.
3, in the greenhouse of 30 meters × 6 meters × 3 meters, choose 3 pieces, the plot of 5 meters × 2 meters, in setting contaminated soil, the concentration of arsenic is respectively 125mg/kg, 25mg/kg, 5mg/kg.
4, in arsenic contaminated soil, lay the earthworm culture medium of 20 cm thicks, culture medium prescription is: fresh cow dung 80%, agricultural residues powder 20%, beneficial organism active bacteria liquid EM bacterium liquid (being 1: 200 by weight proportion), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, every square metre is placed earthworm is 20,000, and the body weight of earthworm is 350 milligrams, for there being the healthy earthworm of reproduction endless belt.
5, the cultivation temperature 23 DEG C of earthworm is kept, in earthworm life layer, moisture accounts for 70% (weight), pH7.1, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
6, repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
7, use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal arsenic is separated with the soil after reparation;
Two, result of the test:
Repair after 6 months, detect the concentration of Arsenic in Soil, the concentration of Arsenic in Soil is respectively 118.06mg/kg, 12.47mg/kg, 1.29mg/kg.
Embodiment 4 earthworm repairs the method for chromium-polluted soil
One, test procedure:
1, using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; Chromium-polluted soil is carried out to the enrichment experiment of heavy metal;
2, soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse size: 30 meters × 6 meters × 3 meters, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, and top covers black external sunshade cloth, facilitates cooling in summer.
3, in the greenhouse of 30 meters × 6 meters × 3 meters, choose 3 pieces, the plot of 5 meters × 2 meters, in setting contaminated soil, the concentration of chromium is respectively 125mg/kg, 25mg/kg, 5mg/kg.
4, on the soil of pollution of chromium, lay the earthworm culture medium of 20 cm thicks, culture medium prescription is: fresh cow dung 80%, agricultural residues powder 20%, beneficial organism active bacteria liquid EM bacterium liquid (being 1: 200 by weight proportion), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, every square metre is placed earthworm is 20,000, and the body weight of earthworm is 350 milligrams, for there being the healthy earthworm of reproduction endless belt.
5, the cultivation temperature 23 DEG C of earthworm is kept, in earthworm life layer, moisture accounts for 70% (weight), pH7.1, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
6, repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
7, use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal chromium is separated with the soil after reparation;
Two, result of the test:
Repair after 6 months, detect the concentration of Chromium in Soil, the concentration of Chromium in Soil is respectively 114.97mg/kg, 18.3mg/kg, 4.43mg/kg.
The method of embodiment 5 earthworm remediating lead-contaminated soil
One, test procedure:
1, using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; Lead-contaminated soil is carried out to the enrichment experiment of heavy metal;
2, soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse size: 30 meters × 6 meters × 3 meters, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, and top covers black external sunshade cloth, facilitates cooling in summer.
3, in the greenhouse of 30 meters × 6 meters × 3 meters, choose 3 pieces, the plot of 5 meters × 2 meters, in setting contaminated soil, plumbous concentration is respectively 125mg/kg, 25mg/kg, 5mg/kg.
4, on the soil of lead contamination, lay the earthworm culture medium of 20 cm thicks, culture medium prescription is: fresh cow dung 80%, agricultural residues powder 20%, beneficial organism active bacteria liquid EM bacterium liquid (being 1: 200 by weight proportion), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, every square metre is placed earthworm is 20,000, and the body weight of earthworm is 350 milligrams, for there being the healthy earthworm of reproduction endless belt.
5, the cultivation temperature 23 DEG C of earthworm is kept, in earthworm life layer, moisture accounts for 70% (weight), pH7.1, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
6, repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
7, use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal lead is separated with the soil after reparation;
Two, result of the test:
Repair after 6 months, detect the concentration of Lead In Soil, the concentration of Lead In Soil is respectively 119.91mg/kg, 14.8mg/kg, 14.93mg/kg.
Claims (9)
1. utilize a method for earthworm restoration of soil polluted by heavy metal, it is characterized in that, using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as biological subject; Contaminated soil simple type single the monofilm greenhouse needing to repair is covered, greenhouse span: 6-8 rice, shoulder height: 1.5-2 rice, rise 2.5-3.5 rice, arch spacing 0.6-1 rice, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, top covers black external sunshade cloth, facilitates cooling in summer.Film is determined by zinc-plated draw-in groove and wire clamping, and greenhouse material all adopts galvanizing to soak; Before reparation, detect the heavy metal concentration in contaminated soil; Contaminated soil is laid the earthworm culture medium of 20 cm thicks; Mixed by 25 centimetres, the top layer Soil tillage of culture medium and heavy-metal contaminated soil, form the earthworm life layer of 45 cm thicks, the repair action that Earthworm on Heavy Metal in Soil pollutes mainly carries out in this region; Every square metre is placed earthworm is 10,000-3 ten thousand, and the body weight of earthworm is 300-500 person of outstanding talent gram, for there being the healthy earthworm of reproduction endless belt; Keep the cultivation temperature 15 ~ 28 DEG C of earthworm in booth, in earthworm life layer, moisture accounts for 55-75% (weight), pH5.5 ~ 8, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth; Repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, under aforementioned cultivating condition, continue reparation 3 months; Use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal is separated with soil; Detect the concentration of heavy metal in soil after repairing; If heavy metal in soil concentration does not still reach the requirement of reparation, to continue under aforementioned cultivating condition with 3 months as the cycle carries out reparation.
2. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 1, is characterized in that, specifically carries out as follows:
(1) the choosing of test organism (earthworm)
Using a kind of earthworm and Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment; Heavy metal contaminated soil carries out the enrichment experiment of heavy metal;
(2) contaminated soil environmental control equipment
Soil simple type single the monofilm greenhouse needing to repair is covered, and this product is a kind of simple type single monofilm greenhouse.This greenhouse has the features such as structure is simple, easy for installation, economical and practical, belong to economical greenhouse, greenhouse span: 6-8 rice, shoulder height: 1.5-2 rice, rises 2.5-3.5 rice, arch spacing 0.6-1 rice, domestic 0.10 millimeters thick plastic sheeting, arrange in both sides and manually roll up film, ventilation equipment, top covers black external sunshade cloth, facilitates cooling in summer.Film is determined by zinc-plated draw-in groove and wire clamping, and greenhouse material all adopts galvanizing to soak, both artistic and practical, corrosion resistant.Practical, service life can reach more than 10 years.Greenhouse size: 30 meters × 6 meters × 2.5 meters; Two, 30 meters × 6 meters × 3 meters; Three, 32 meters × 8 meters × 3.35 meters.
(3) heavy metal in soil Concentration Testing before repairing
Before reparation, detect the concentration of Heavy Metals in Contaminated Soils.
(4) earthworm life layer is set
The soil of heavy metal pollution is laid the earthworm culture medium of 20 cm thicks, culture medium optimum formula is: fresh cow dung 75-85%, agricultural residues powder 15-25%, beneficial organism active bacteria liquid EM bacterium liquid (1: 200), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, the repair action of Soil of Earthworms arable layer heavy metal pollution mainly carries out in this region; Every square metre is placed earthworm is 10,000-3 ten thousand, and the body weight of earthworm is 300-500 milligram, for there being the healthy earthworm of reproduction endless belt.
(5) repair process earthworm condition of culture is arranged
Keep the cultivation temperature 15 ~ 28 DEG C of earthworm, in earthworm life layer, moisture accounts for 55-75% (weight), pH5.5 ~ 8, and salinity is less than 0.02% (volume), ammonia is less than 25% (volume), and sulfuration poison gas is less than 15% (volume).Red light source illumination is used in booth.
(6) repairing efficiency is arranged
Repair after 3 months, re-lay the culture medium of 20 cm thicks on top layer, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
(7) heavy metal in soil Concentration Testing after repairing
Use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal is separated with the soil after reparation; Detect the concentration of heavy metal in soil after repairing, if the concentration of heavy metal in soil does not still reach the requirement of reparation, to continue under aforementioned cultivating condition with 3 months as one-period reparation.
3. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 2, is characterized in that, in said method, using Eisenia Foetida (Eiseniafoetida) as the biology of enrichment experiment in step (1).
4. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 2, it is characterized in that, in step (2), rehabilitating soil greenhouse used is a kind of simple type single monofilm greenhouse, this greenhouse span: 6-8 rice, shoulder height: 1.5-2 rice, rises 2.5-3.5 rice, arch spacing 0.6-1 rice, domestic 0.10 millimeters thick plastic sheeting, arranges in both sides and manually rolls up film, ventilation equipment, and napex covers black external sunshade cloth.Greenhouse size: 30 meters × 6 meters × 2.5 meters; Two, 30 meters × 6 meters × 3 meters; Three, 32 meters × 8 meters × 3.35 meters.
5. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 2, is characterized in that, before repairing, detects the concentration of Heavy Metals in Contaminated Soils in step (3).
6. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 2, it is characterized in that, on the soil of heavy metal pollution, the earthworm culture medium of 20 cm thicks is laid described in step (4), culture medium optimum formula is: fresh cow dung 75-85%, agricultural residues powder 15-25%, beneficial organism active bacteria liquid EM bacterium liquid (1: 200), form after 15 days through anaerobic fermentation; Mixed by the Soil tillage on 25 centimetres, the top layer of culture medium and heavy-metal composite pollution, form the earthworm life layer of 45 cm thicks, the repair action of Soil of Earthworms arable layer heavy metal pollution mainly carries out in this region; Every square metre is placed earthworm is 10,000-3 ten thousand, and the body weight of earthworm is 300-500 milligram, for there being the healthy earthworm of reproduction endless belt.
7. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 2, it is characterized in that, repair process earthworm condition of culture described in step (5) is set to keep in the cultivation temperature 15 ~ 28 DEG C of earthworm, earthworm life layer that moisture accounts for 55-75% (weight), pH5.5 ~ 8, salinity is less than 0.02% (volume), ammonia is less than 25% (volume), sulfuration poison gas is less than 15% (volume), use red light source to throw light in booth.
8. the method utilizing earthworm restoration of soil polluted by heavy metal according to claim 2, it is characterized in that, repairing efficiency described in step (6) is reparation re-lays 20 cm thicks on top layer culture medium after 3 months, culture medium prescription, as front, continues reparation 3 months under aforementioned cultivating condition.
9. the method that Heavy Metal Ecological toxicity in water body deposit is evaluated according to claim 2, it is characterized in that, heavy metal in soil Concentration Testing after repairing described in step (7), use light source to drive the life layer being stripped to earthworm gradually by top layer, the earthworm being enriched heavy metal is separated with the soil after reparation; Detect the concentration of heavy metal in soil after repairing, if the concentration of heavy metal in soil does not still reach the requirement of reparation, to continue under aforementioned cultivating condition with 3 months as one-period reparation.
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| CN106831021A (en) * | 2017-02-10 | 2017-06-13 | 王均猛 | A kind of method that harmless treatment is carried out to house refuse |
| CN110404958A (en) * | 2019-06-19 | 2019-11-05 | 上海市环境科学研究院 | A method of utilizing Trichoderma and earthworm interaction remediating heavy metal and organic pollutant combined contamination soil |
| CN110153161A (en) * | 2019-07-09 | 2019-08-23 | 上海建为环保科技有限公司 | The technical method of metallic pollution soil is repaired in a kind of animals and plants collaboration |
| CN111229807A (en) * | 2019-10-18 | 2020-06-05 | 杭州罗松贸易有限公司 | Soil remediation method |
| CN111922073A (en) * | 2020-07-29 | 2020-11-13 | 陆炯 | Biological method soil remediation system and soil remediation method |
| CN112453035A (en) * | 2020-11-02 | 2021-03-09 | 云南大学 | Ecological detection system and method for restoring heavy metals in soil by using bred earthworms |
| CN112642858A (en) * | 2020-12-04 | 2021-04-13 | 张宁 | Soil remediation ecological net and remediation method thereof |
| CN112673743A (en) * | 2020-12-07 | 2021-04-20 | 织金县慷骅农资集团有限公司 | Method for remedying heavy metal pollution of farmland |
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