CN102550452B - Cultivating method of Sea anemone parents - Google Patents

Cultivating method of Sea anemone parents Download PDF

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Publication number
CN102550452B
CN102550452B CN2011104588678A CN201110458867A CN102550452B CN 102550452 B CN102550452 B CN 102550452B CN 2011104588678 A CN2011104588678 A CN 2011104588678A CN 201110458867 A CN201110458867 A CN 201110458867A CN 102550452 B CN102550452 B CN 102550452B
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China
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parent
sea anemone
culture tank
culturing rack
water
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CN102550452A (en
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杨海萍
吴建平
刘海林
张志勇
沈德华
许津
徐献民
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Institute Of Oceanology & Marine Fisheries Jiangsu
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Institute Of Oceanology & Marine Fisheries Jiangsu
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention relates to a cultivating method of sea anemone parents, comprising the following steps: collecting sea anemones which are healthy, vigorous, and unharmed, and have complete tentacles and intact foots; placing the anemones in a parent cultivating system to make the anemones spawn and produce milt; collecting fertilized eggs after the gametes being fertilized; and transferring the fertilized eggs to a nursery pond to perform embryo cultivation and breed. According to the invention, the cultivating method of sea anemone parents has advantages that the feeding is convenient to manage, the cultivating method is convenient for long-term cultivation and is easy to observe, the cultivating effect is good, the water is recycled, the fertilized eggs are collected continuously and the like, the technical difficulty that the sinkable fertilized eggs from anemones in a life form collected by digging are hard to collect efficiently is solved, the hatching rate of the fertilized eggs is high, the residual feeds and the undigested residuals are easy to clear away, and the cultivating method has strong operability and good practicability.

Description

A kind of cultural method of sea anemone parent
Technical field
The invention belongs to aquaculture field, particularly a kind of sea anemone parent cultural method.
Background technology
The celestial shadow sea anemone of China is individual big, be of high nutritive value, tasty mouthfeel, be a kind of high-grade edible aquatic products, the toxin of sea anemone can be used for hypotensive, blood coagulation, and cardiac nerve and muscle are had analgesic effect, medical value with the calmness of calming the nerves, pain relieving, anti-ageing, anticancer change, the celestial shadow sea anemone of China also is the biotoxin research object and views and admires the Shui nationality, natural distribution is arranged in that China is coastal, but natural resources seldom.Chinese celestial shadow sea anemone commodity rely on fully to adopt and catch natural resources at present, can not meet the need of market, and the research of carrying out artificial propagation and cultural technique is the inevitable approach of aquatic products industry development.
Carry out parent and cultivate, make its ripe spawning and fertilization, obtaining fertilized egg is the basis of artificial propagation.The research of China's celestial shadow sea anemone artificial propagation still is in initial stage, and domestic have only Jiangsu Prov. Inst. of Marine Aquatic Products to carry out this work.The celestial shadow sea anemone of China is sought the life of excavation type in silt, make a living by the minitype aquatic animal of swimming across in tentacle and the nematoblast passivity predation water.Residual bait and digestion residue easy-clear not when the simulation natural, ecological adopts the silt substrate to cultivate under the artificial condition, long-term cultivation can cause substrate to worsen and death, the celestial shadow sea anemone fertilized egg of China is demersal egg, conventional method is difficult to fertilized egg is separated with the silt that constitutes substrate, and the collection of fertilized egg becomes the technical bottleneck of artificial propagation.
Summary of the invention
Problem to be solved by this invention provides a kind of sea anemone parent cultural method, and its technical scheme may further comprise the steps:
A. parent collection
Gather the sea anemone parent, prepare parent and cultivate;
B. set up the parent culture systems, comprising:
The parent culture tank, culturing rack, parent culture tank, outlet pipe, residual bait and digestion residue separating tank, collection ovum net cage, egg collecting groove, water inlet pipe, the sea anemone parent, gas exhausting stone, air pump and gas circuit, water pump and pipeline, wherein: the sea anemone parent is incubated in the parent culture tank, and the parent culture tank places culturing rack, and culturing rack is placed in the parent culture tank, current flow into residual bait and digestion residue separating tank by outlet pipe, fertilized egg flows into collection ovum net cage with water, and collection ovum net cage places egg collecting groove, and water body is through water pump and pipeline, water inlet pipe flows into the parent culture tank, air pump and gas circuit connect gas exhausting stone, give the gas exhausting stone air feed.
Described a kind of sea anemone parent cultural method is characterized in that: described sea anemone parent is Chinese celestial shadow sea anemone parent.
Described a kind of sea anemone parent cultural method is characterized in that: the sea anemone parent of steps A collection is at the health of gathering the 3-4 month, energetic, not damaged, tentacle is complete, foot is intact sea anemone parent.
Described a kind of sea anemone parent cultural method, it is characterized in that: the parent culture tank is for circular in the described system of step B, center bottom is mild taper, culture tank central authorities establish parent and intercept tube, central water stream intercepts tube by parent and flows into outlet pipe through central delivery port, gas exhausting stone generation gas lift effect in parent intercepts tube makes in the parent culture tank and forms vertical convection, and water inlet pipe is along the tangential direction setting suitable for reading of parent culture tank, and water pump and pipeline make water inlet pipe cause jet to form horizontal circulation.
Described a kind of sea anemone parent cultural method, it is characterized in that: also comprise annular culturing rack module and fan-shaped culturing rack module in the described system of step B, wherein 1 annular culturing rack module becomes culturing rack integral body to be placed in the parent culture tank with a plurality of fan-shaped culturing rack module combinations, a plurality of parent culture tank evenly are installed on the culturing rack, regulate water pump and pipeline flow velocity and keep water level.
Described a kind of sea anemone parent cultural method is characterized in that: wherein 1 annular culturing rack module becomes culturing rack integral body to be placed in the parent culture tank with 8 fan-shaped culturing rack module combinations, and 160 parent culture tank evenly are installed on the culturing rack.
Described a kind of sea anemone parent cultural method is characterized in that: the sea anemone bait of manually throwing something and feeding is ingested for the sea anemone parent, and residual bait and digestion residue enter residual bait and digestion residue separating tank with current.
Described a kind of sea anemone parent cultural method is characterized in that: dissolved oxygen amount in the egg collecting groove water body is kept in gas exhausting stone inflation in bottom, guarantees that fertilized egg reaches high hatchability in fine water.
The celestial shadow sea anemone parent culture systems beneficial effect of the present invention China:
This system solves the difficult problem that fertilized egg is collected, and has avoided conventional method need use silt to do substrate, makes residual bait be easy to separate removal with the digestion residue, but have long-term cultivation, be easy to management, fertilized egg is collected continuously, the advantage that incubation rate is high has a good application prospect.Of the present invention promoting the use of will promote the development of sea anemone artificial propagation and aquaculture.
Description of drawings
Fig. 1 is parents culture system side view
Fig. 2 is parents culture groove vertical view
Fig. 3 is the modular structure schematic diagram
Reference numeral: 1 parent culture tank, 2 culturing racks, 3 parent culture tank, 4 parents intercept tube, 5 outlet pipes, 6 residual baits and digestion residue separating tank, 7 collection ovum net cages, 8 egg collecting grooves, 9 water inlet pipes, 10 water levels, 11 sea anemone parents, 12 gas exhausting stones, 13 air pumps and gas circuit, 14 water pumps and pipeline, 15 vertical convections, 16 central delivery ports, 17 central current, 18 horizontal circulation, 19 annular culturing rack modules, 20 fan-shaped culturing rack modules.
Embodiment
Below by example the present invention is described in further detail, but the present invention is not limited to following example
Implementation step:
The a parent is gathered, and adopts from natural waters and catches wild Chinese celestial shadow sea anemone adult, and it is strong to select health, broken wound, and tentacle is complete, and the Chinese celestial shadow sea anemone that foot finishes is as parent;
B places the parent culture tank to cultivate parent, uses filtering sea, seawater salinity 20-26 ‰, PH7.8-8.6.Start water pump and air pump;
Bait throwing in every day when the c water temperature is increased to more than 14 ℃ is removed foreign material in residual bait and the digestion residue separating tank every day, and is cleaned separating tank;
The d water temperature is increased to 24 ℃ more than-26 ℃ the time, and the sea anemone parent begins maturation lays eggs, and collect fertilized egg from collection ovum net cage every day, avoids fertilized egg dried up when getting ovum.
System produces current circulates water body between parent culture tank → residual bait and digestion residue separating tank → egg collecting groove → parent culture tank.Lay eggs and collect fertilized egg back every day.
System uses the garden shape fiberglass tank of 2 meters of diameters as the parent culture tank, the bottom is for slow tapered, make culturing rack and culture tank with non-toxic plastic, culturing rack adopts modularized design, be convenient to dismounting and operational administrative, use inflation to cause the gas lift effect to make at the obstruct tube and produce vertical convection in the culture tank, water pump power produces the tangent line jet and forms horizontal circulation, make fertilized egg and residual bait and digestion residue dynamically enter central delivery port, remove foreign material through residual bait and digestion residue separating tank, fertilized egg enters collection ovum net cage, collection ovum net cage adopts 200 mesh sieve thin,tough silk to make etting, spawning season is collected fertilized egg from collection ovum net cage every day, and seawater is through water pump and pipeline suction parent culture tank in the egg collecting groove, and water body recycles.
Embodiment
Jiangsu Prov. Inst. of Marine Aquatic Products adopted from natural waters and caught 320 of wild Chinese celestial shadow sea anemone adults and place 2 parent culture tanks to cultivate as parent in April, 2011, added filtering sea, seawater salinity 20-26 ‰, PH7.8-8.6.Bait throwing in every day when water temperature is increased to more than 14 ℃ is removed foreign material in residual bait and the digestion residue separating tank every day, and is cleaned separating tank.Cultivate through 3 months, water temperature is increased to 24 ℃ more than-26 ℃ the time after June, and the maturation row that begins to lay eggs is smart gradually for parent, collects fertilized egg in the collection ovum net cage every day and is used for hatching and grows seedlings 1,400 ten thousand, cultivates into the floating wave larva through hatching more than 90%.
Below only expressed embodiments of the present invention, it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (7)

1. sea anemone parent cultural method may further comprise the steps:
A. parent collection:
Gather the sea anemone parent, prepare parent and cultivate;
B. set up the parent culture systems, comprising:
Parent culture tank (1), culturing rack (2), parent culture tank (3), outlet pipe (5), residual bait and digestion residue separating tank (6), collection ovum net cage (7), egg collecting groove (8), water inlet pipe (9), sea anemone parent (11), gas exhausting stone (12), air pump and gas circuit (13), water pump and pipeline (14), wherein: sea anemone parent (11) is incubated in the parent culture tank (3), and parent culture tank (3) places culturing rack (2), and culturing rack (2) is placed in the parent culture tank (1), current flow into residual bait and digestion residue separating tank (6) by outlet pipe (5), fertilized egg flows into collection ovum net cage (7) with water, and collection ovum net cage (7) places egg collecting groove (8), and water body is through water pump and pipeline (14), water inlet pipe (9) flows into the parent culture tank, air pump and gas circuit (13) connect gas exhausting stone (12), give gas exhausting stone (12) air feed;
Parent culture tank (1) is circular in the described system of step B, center bottom is mild taper, culture tank (1) central authorities establish parent and intercept tube (4), central authorities' current (17) intercept tube (4) by parent and flow into outlet pipe (5) through central delivery port (16), gas exhausting stone (12) produces the gas lift effect and makes formation vertical convection (15) in the parent culture tank (1) in parent intercepts tube (4), water inlet pipe (9) is along parent culture tank (1) tangential direction setting suitable for reading, and water pump and pipeline (14) make water inlet pipe (9) cause jet to form horizontal circulation (18).
2. a kind of sea anemone parent cultural method according to claim 1, it is characterized in that: described sea anemone parent is Chinese celestial shadow sea anemone parent.
3. a kind of sea anemone parent cultural method according to claim 1 is characterized in that: the sea anemone parent of steps A collection is at the health of gathering the 3-4 month, energetic, not damaged, tentacle is complete, foot is intact sea anemone parent.
4. according to arbitrary described a kind of sea anemone parent cultural method in the claim 1 to 3, it is characterized in that: also comprise annular culturing rack module (19) and fan-shaped culturing rack module (20) in the described system of step B, wherein 1 annular culturing rack module (19) and a plurality of fan-shaped culturing rack modules (20) are combined into culturing rack (2) integral body and are placed in the parent culture tank (1), a plurality of parent culture tank (3) evenly are installed on the culturing rack (2), regulate water pump and pipeline (14) flow velocity and keep water level (10).
5. a kind of sea anemone parent cultural method according to claim 4, it is characterized in that: wherein 1 annular culturing rack module (19) and 8 fan-shaped culturing rack modules (20) are combined into culturing rack (2) integral body and are placed in the parent culture tank (1), and 160 parent culture tank (3) evenly are installed on the culturing rack (2).
6. according to arbitrary described a kind of sea anemone parent cultural method in the claim 1 to 3, it is characterized in that: the sea anemone bait of manually throwing something and feeding is ingested for sea anemone parent (11), and residual bait and digestion residue enter residual bait and digestion residue separating tank (6) with current.
7. according to arbitrary described a kind of sea anemone parent cultural method in the claim 1 to 3, it is characterized in that: dissolved oxygen amount in egg collecting groove (8) water body is kept in bottom gas exhausting stone (12) inflation, guarantees that fertilized egg reaches high hatchability in fine water.
CN2011104588678A 2011-12-31 2011-12-31 Cultivating method of Sea anemone parents Expired - Fee Related CN102550452B (en)

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CN102972347A (en) * 2012-12-16 2013-03-20 向玉堂 Tortoise or trionyx sinensis spawning pond
CN104304112A (en) * 2014-10-11 2015-01-28 大连海洋大学 Sea cucumber gamete collection device
CN104938386A (en) * 2015-07-01 2015-09-30 谢新民 Inflation oxygenation and water saving type temperature-controllable aquatic animal incubation device
CN108363441B (en) * 2018-01-12 2023-08-15 浙江大学 Manual upflow system and regulation and control method based on manual upflow oligotrophic salt sea area
CN111758623A (en) * 2020-07-10 2020-10-13 海南德益丰水产科技有限公司 Method and system for high-density culture of clean rotifers
CN112293317B (en) * 2020-11-19 2022-07-19 中国水产科学研究院黑龙江水产研究所 Method for detecting hatching rate of fertilized eggs in salmon spawning site under natural condition
CN113367086A (en) * 2021-03-17 2021-09-10 广东海洋大学 Method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs

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