CN113367086A - Method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs - Google Patents
Method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs Download PDFInfo
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- CN113367086A CN113367086A CN202110284751.0A CN202110284751A CN113367086A CN 113367086 A CN113367086 A CN 113367086A CN 202110284751 A CN202110284751 A CN 202110284751A CN 113367086 A CN113367086 A CN 113367086A
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- eggs
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- demersal
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 94
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000013020 embryo development Effects 0.000 claims abstract description 13
- 241000238557 Decapoda Species 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 8
- 238000011534 incubation Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 5
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241001417871 Silurus asotus Species 0.000 claims abstract description 4
- 238000002474 experimental method Methods 0.000 claims abstract description 4
- 230000000877 morphologic effect Effects 0.000 claims abstract description 4
- 239000013535 sea water Substances 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 4
- 241000746809 Silurus meridionalis Species 0.000 claims description 3
- 210000002257 embryonic structure Anatomy 0.000 claims description 3
- 230000026109 gonad development Effects 0.000 claims description 3
- 230000001418 larval effect Effects 0.000 claims description 3
- 241000347488 Silurus Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 5
- 210000002149 gonad Anatomy 0.000 abstract description 3
- 230000003203 everyday effect Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000007547 defect Effects 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 241000252073 Anguilliformes Species 0.000 description 1
- 241001466864 Clarias fuscus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the technical field of fish egg collection, separation, incubation, identification and classification, and discloses a natural marine demersal fish egg living egg collection, separation, incubation, identification and classification method, which comprises the following steps: catching parent fish in the breeding period, and obtaining sinking eggs by an artificial breeding method after catching the parent fish with mature gonads; performing embryo development observation by using the produced eggs, temporarily culturing the captured male individuals and female individuals in cylindrical shrimp cages separately one by one, hanging and culturing on the sea, and respectively checking whether the male individuals and the female individuals lay eggs in the morning and at the evening every day; the produced eggs are directly packed by filling pure oxygen into shrimp seed bags and then are taken back to a laboratory for embryo development observation and other experiments. The method for collecting, separating, incubating, identifying and classifying the live eggs of the natural marine demersal fish eggs provided by the invention can establish a morphological identification standard of the live eggs of Chinese silurus asotus and effectively solve the problem that the traditional marine demersal eggs are difficult to collect.
Description
Technical Field
The invention belongs to the technical field of collection, separation, incubation, identification and classification of fish eggs, and particularly relates to a method for collection, separation, incubation, identification and classification of live natural marine demersal fish eggs.
Background
At present, early marine organism resources and supplement mechanisms are important contents of marine resource investigation and environmental evaluation, and the horizontal and vertical sampling method of the common marine plankton trawl only can collect floating eggs, drifting eggs and a small amount of free sticky fish eggs due to the limitation of an operation mode, but cannot collect corresponding fish eggs for fish producing sinking eggs, so the investigation by using the horizontal and vertical sampling method of the marine plankton trawl cannot be carried out. Therefore, a new method for collecting, separating, hatching, cultivating, identifying and classifying the live eggs of the natural marine demersal fish eggs is needed.
Through the above analysis, the problems and defects of the prior art are as follows: the traditional marine demersal egg collection method can only collect floating eggs, drifting eggs and a small amount of free viscous fish eggs, and can not collect corresponding fish eggs for demersal egg fishes.
The difficulty in solving the above problems and defects is:
after the demersal ova lay eggs, because of the physical characteristics of the ova, the specific gravity of the ova is greater than that of seawater, the ova sink to the seabed and cannot be collected by a trawl directly on the water surface or the water bottom, the larvae of the demersal ova are collected by the puppies in the larval and juvenile investigation carried out by the Japanese in the 1990 s, the special collection method for the demersal ova marine ova is few, the collection method for the demersal ova has large damage to fertilized ova, and the requirements on equipment and operation are high. The relevant reports of marine fish demersal egg collection are few.
The significance of solving the problems and the defects is as follows: chinese patent CN205912698U discloses a fertilized egg collecting device for seawater cage cultured fish; japanese patent J P200-35772A discloses a method for collecting eggs of eels: the trawl is driven by the ship to move on the seabed, and the water spraying pipe is arranged in front of the trawl and sprays water to bottom sand on the seabed, so that the eggs buried in the sand are stirred and are collected by the trawl at the rear end; in the 80 s of the 20 th century, soviet union patent application SU1398784a1 disclosed an inverted triangular egg collection device: the net cage is characterized in that sinking eggs are collected in a water pumping mode, a conical fish egg collector is arranged at the bottom of the net cage and connected into an egg collecting box through a hose, and water in the fish egg collector and fish eggs are conveyed into the egg collecting box through the hose under the action of a pump. The method for collecting eel eggs used in japanese patent No. J P200-35772A requires complicated equipment and operation, and easily scrapes fertilized eggs, and does not easily separate the fertilized eggs from other aquatic animals or impurities mixed in the net; the method for collecting the sinking eggs of the Su-Union patent application SU1398784A1 is only suitable for a method for collecting a large amount of sinking eggs in net cage culture, the sinking eggs collected by the two methods are not easy to collect for sinking eggs of fishes such as Chinese silurus asotus with large egg diameter and small fecundity, and the invention is a method for collecting the sinking eggs capable of artificially culturing sexual maturity parent fishes or naturally capturing the sexual maturity parent fishes.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for collecting, separating, incubating, identifying and classifying live eggs of natural marine sinking fish eggs.
The invention is realized in such a way that the method for collecting, separating, incubating, culturing, identifying and classifying the live eggs of the natural marine demersal fish eggs comprises the following steps:
step one, using a cage net to trap corresponding parent fishes in a breeding period, capturing the parent fishes with mature gonads, and then obtaining sinking eggs by an artificial breeding method;
step two, enabling adult fish of the Chinese silurus meridionalis to be large in size and small in size, temporarily breeding the captured male individuals and female individuals, namely male individuals and female individuals, in a cylindrical shrimp cage respectively, hanging and breeding the male individuals and the female individuals on the sea, obtaining spawning of parent fish by using an artificial spawning induction or natural spawning method, and performing subsequent embryo development observation by using the produced spawns;
and step three, directly filling pure oxygen into the produced eggs by using shrimp seed bags, directly packaging and taking the eggs back to a laboratory for embryo development observation and other experiments.
Further, in the first step, the method for obtaining the sinking eggs comprises the following steps: artificial spawning is induced or spawned naturally by artificial propagation method to obtain sinking egg directly.
Further, in the second step, seawater with stable temperature and salinity is continuously aerated to observe the embryo development of the eggs, and the eggs are photographed once every 2 to 3 days until the embryos hatch.
Further, after photographing the newly hatched fries, establishing a morphological identification standard of live eggs of Chinese silurus asotus through COI molecular identification of the newly hatched fries and adult fishes.
Further, in the second step, the diameter of the cylindrical shrimp cage is 50 cm.
By combining all the technical schemes, the invention has the advantages and positive effects that: the invention provides a method for collecting, separating, incubating, culturing, identifying and classifying live eggs of natural marine sinking fish eggs, which aims at the difficulty of collecting the traditional marine sinking eggs, directly catches corresponding parent fish in a breeding period, catches the parent fish with mature gonads, and then carries out artificial spawning hastening or natural spawning by an artificial breeding method to directly obtain the sinking eggs, thereby being capable of utilizing the produced eggs to carry out subsequent embryo development observation.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flow chart of a method for collecting, separating, hatching, cultivating, identifying and classifying live eggs of natural marine demersal fish eggs according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs, and the method is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for collecting, separating, hatching, cultivating, identifying and classifying live eggs of natural marine demersal fish eggs provided by the embodiment of the present invention includes the following steps:
s101: trapping corresponding parent fish by using a cage net in a breeding period, capturing the parent fish with mature gonad development, and obtaining sinking eggs by an artificial breeding method;
s102: adult fish of the silurus meridionalis is large in size and small in size, captured male individuals and female individuals, namely male individuals and female individuals, are respectively and temporarily cultured in cylindrical shrimp cages separately and hung on the sea, parent fish spawning is obtained by using an artificial induced spawning or natural spawning method, and the produced spawns are used for subsequent embryo development observation;
s103: the produced eggs are directly packed by filling pure oxygen into shrimp seed bags and then are taken back to a laboratory for embryo development observation and other experiments.
The technical solution of the present invention is further described with reference to the following examples.
Aiming at the difficulty of traditional marine sinking egg collection, the method directly captures corresponding parent fish in a breeding period, after capturing the parent fish with mature gonad development, artificially hastens parturition or naturally spawns by an artificial breeding method, and directly obtains sinking eggs, so that the produced eggs can be used for subsequent embryo development observation.
Continuously inflating seawater with stable temperature and salinity, observing embryo development of eggs, photographing once at regular intervals until the embryos are hatched, photographing the primarily hatched larvae, and identifying COI molecules of the primarily hatched larvae and adult fish to establish a morphological identification standard of live eggs of the Chinese Clarias fuscus.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs is characterized by comprising the following steps:
trapping corresponding parent fish by using a cage net in a breeding period, capturing the parent fish with mature gonad development, and obtaining sinking eggs by an artificial breeding method;
adult fish of the silurus meridionalis is large in size and small in size, captured male individuals and female individuals, namely male individuals and female individuals, are respectively and temporarily cultured in cylindrical shrimp cages separately and hung on the sea, parent fish spawning is obtained by using an artificial induced spawning or natural spawning method, and the produced spawns are used for subsequent embryo development observation; the produced eggs are directly packed by filling pure oxygen into shrimp seed bags and then are taken back to a laboratory for embryo development observation and other experiments.
2. The method for collecting, separating, hatching, cultivating, identifying and classifying the live eggs of natural marine demersal fish eggs as claimed in claim 1, wherein the method for obtaining the demersal eggs comprises: artificial spawning is induced or spawned naturally by artificial propagation method to obtain sinking egg directly.
3. The method for collecting, separating, incubating, identifying and classifying the living eggs of natural marine demersal fish as claimed in claim 1, wherein seawater with stable temperature and salinity is continuously aerated to observe the embryo development of the eggs, and the images are taken every 2-3 days until the embryos are incubated.
4. The method for collection, separation, incubation, cultivation, identification and classification of live eggs of natural marine silurus asotus of claim 3, wherein after photographing the pre-incubated larval fish, the morphological identification standard of live eggs of silurus zhonghuashanensis is established by identifying COI molecules of the pre-incubated larval fish and adult fish.
5. The method for collecting, separating, incubating, identifying and classifying the live eggs of the natural marine demersal fish as claimed in claim 1, wherein the diameter of the cylindrical shrimp cage is 50 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110284751.0A CN113367086A (en) | 2021-03-17 | 2021-03-17 | Method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110284751.0A CN113367086A (en) | 2021-03-17 | 2021-03-17 | Method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs |
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| CN113367086A true CN113367086A (en) | 2021-09-10 |
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| CN202110284751.0A Pending CN113367086A (en) | 2021-03-17 | 2021-03-17 | Method for collecting, separating, incubating, identifying and classifying live eggs of natural marine demersal fish eggs |
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2021
- 2021-03-17 CN CN202110284751.0A patent/CN113367086A/en active Pending
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Application publication date: 20210910 |