CN113498748B - Method for calculating survival rate of drifting roes according to survival rates of roes at different stages of development - Google Patents
Method for calculating survival rate of drifting roes according to survival rates of roes at different stages of development Download PDFInfo
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Abstract
The invention discloses a method for calculating the survival rate of driftage roes according to the survival rate of the roes at different stages of development, which comprises the following steps: collecting a drifting roe sample; culturing the collected sample by an artificial culture mode; separating different kinds of roe individuals and culturing respectively; manually sampling the separated cultured and developed fish eggs; observing the development period of the sampled roe embryo under a microscope, and recording the survival rate of the roe; continuously sampling, observing and recording the development stage and survival rate of roes at each development stage; multiplying the survival rates of the fish eggs at different development stages to obtain the total survival rate of the fish eggs.
Description
Technical Field
The invention relates to the field of fishery breeding, in particular to a method for calculating the survival rate of drifting roes according to the survival rate of the roes at different stages of development.
Technical Field
With the increasing attention paid to the problem of marine sustainable development, the research on the dynamics of marine ecosystem and the sustainable utilization of biological resources has become the international frontier field of the current marine interdisciplinary research. The dynamics and changes of fish resources depend on the strength of their generation, which depends to a large extent on the status of replenishment at their early stages of life history. The survival and quantity of roes are the basis of fish resource supplement and continuous utilization of fishery resources, and the roe stage is the most vulnerable period in the fish life cycle, the drifting performance of roes along with ocean currents and the sensitivity to the ocean environment are realized, the slight change of the ocean environment factors can have strong influence on the development and growth of roes until supplement of the fish population, and the survival rate and the residual quantity of roes at this stage can determine apology the abundance of the fish supplement population resource quantity. Therefore, the research on the survival mechanism and the number of fish eggs is one of the essential first jobs in the research on the sustainable utilization of fishery resources. Secondly, in marine food nets, fish eggs are the main predators. In the research of marine nutrition kinetics, roe is a converter of biological energy and is one of the important links in marine food chain. Therefore, the more important the research on the biology and ecology of roe in the marine ecosystem.
The fish has high reproductive capacity and is an adaptation to low survival rate. Most fish lay eggs in appreciable quantity, but the eggs float in natural waters and complete fertilization in water, a large part of which cannot be fertilized and are often swallowed by other fishes and shrimps, after a storm, the eggs or hatched larval fish are crushed into millions, greedy enemies can damage the eggs during the development of the larval fish, some fishes cannot find baits, some fishes cannot resist water temperature change, and a considerable part of the eggs are eliminated during the growth and development. Thus, hundreds of millions of eggs can develop into big fish after serious 'disasters', and the number of the eggs is very few. Fishes can continue to breed in nature, and are inseparable from some ingenious adaptations to the external environment during their reproduction. The green, grass, silver carp, bighead carp in fresh water and the car-turning fish in sea produce floating eggs, the eggs float on the upper layer of water through the flow of water, the dissolved oxygen and the light are sufficient, the water temperature is often higher than that of the bottom layer, and the conditions are all superior conditions for hatching the floating eggs. Fish in oceans almost lay these eggs, even floatover, which live on the seabed for the last year, also lay floating eggs. The fish producing floating eggs generally have large egg laying amount, which is suitable for large loss and low survival rate. For example, the number of eggs laid by a flounder of 8.5 kg can exceed 900 ten thousand grains; 1.25 kg of mackerel can produce 260 ten thousand grains at most; the small yellow croakers can produce 20-30 ten thousand grains; the egg laying amount of the hairtail is the minimum, but 2.5-3 ten thousand eggs can be generally produced. Therefore, the estimation of the survival rate and survival number of the fish eggs has important significance for the fish resource supplement and the continuous utilization of fishery resources in water areas such as ocean, fresh water and the like.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a method for calculating the survival rate of the floating roe according to the survival rate of the roe at different stages of development, and solves the problem that the survival rate of the roe in the existing water areas such as ocean, fresh water and the like is difficult to estimate.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for calculating the survival rate of drifting roes according to the survival rate of the roes at different stages of development, comprising the following steps:
s1, collecting a drifting roe sample;
s2, culturing the collected sample with roe in an artificial culture mode;
s3 separating different kinds of roe individuals and culturing respectively;
s4, manually sampling the separated cultured and developed fish eggs;
s5, observing the development period of the roe embryo of the sampled roe under a microscope, and recording the survival rate of the roe;
s6, continuously sampling and observing and recording the development period and survival rate of the roe at each development stage;
s7, the survival rates of different development stages of the roe are multiplied to obtain the total survival rate of the roe.
In step S1, the driftage roe sample is marine driftage roe and/or roe and larval fish of inland river; wherein the collection of the ocean drifting fish roe adopts a large-scale plankton made of a silk screen with the aperture of 0.505mm to carry out surface layer horizontal trawl; the method comprises the steps of collecting fish eggs and fish larvae of inland rivers by using a conical net for quantitative collection, and simultaneously using a jiang net for auxiliary qualitative collection.
The artificial culture mode is to temporarily culture the fish eggs collected in the sea or fresh water field in a plastic bucket containing seawater or fresh water, and bring the fish eggs back to a laboratory on the same day for indoor incubation.
The separation of the different kinds of roe individuals is to distinguish the different kinds of roe individuals by utilizing the various quantifiability characters, countable characters and description characters of the different kinds of roe.
It should be noted that quantifiable properties often occur as absolute values or as ratios of lengths of different sites. In the fertilized egg and embryo development stage, the common measurement items include the yolk diameter, the egg membrane diameter, the oil globule diameter, the long diameter and the short diameter of the non-circular egg, the length of the egg membrane silk with the egg membrane silk type, and the like. In the formation of embryo, the length of the embryo in different developmental stages is also one of the bases for identifying the species. The ratio of the size of the eye to the length of the embryo body after the eye is formed; when the tail portion leaves the yolk sac, the ratio of the length of the tail portion to the length of the embryo body is different from species to species. After the embryo body is filmed, a certain morphological structure is formed along with the development of the fish body, and the measurable character is correspondingly increased. In the early development stage of embryoid body, the important characteristics commonly used include the diameter of the egg membrane and the size of the perivitelline space, and the late embryonic stage is the curling degree of the pre-membrane embryo in the egg membrane. The characteristics of the size of the young fish eyes after the film is produced, the ratio of the body height (body width) to the body length, the length of the tail part and the like have higher reference values.
The countable traits include various characteristics that can be directly counted in the early development stage of fish, such as the existence of fertilized eggs and oil globules in the embryo stage, such as the number, the number of muscle nodes, and the like, and the number of muscle nodes of the larval fish after the larval fish is born. In the fertilized egg and embryo stage, the existence and number of oil globules and the existence of ootheca silks have great difference among different species, so that the method can be used for distinguishing different species.
Descriptive traits are a class of comprehensive features. These features are often difficult to represent in a quantitative manner and appear in a textual narrative. Such as the shape and structure of the egg, the color of the yolk, the color of the oil globule, the order of appearance of organs during embryonic development, the body shape and pigment distribution of the fry, the size and body shape of the eye, etc.
In step S5, the survival rate of the roe embryo development is calculated by recording the total number of the sampled roe at this stage and observing the number of the surviving individuals through microscopic examination, thereby obtaining the survival rate of the roe embryo development at this stage.
It should be further noted that in step S5 of the present invention, the development process of the fish egg embryo is roughly divided into five stages. The five stages can be clearly observed and divided through microscopic examination, can also be roughly observed by naked eyes, and can improve the accuracy of observation by combining the time periods of embryo development under different water temperature conditions. Specifically, the method comprises the following steps:
and (4) a cleavage stage. After fertilization of fish eggs, the egg membrane begins to absorb water and swell, and meanwhile cytoplasm gradually concentrates and protrudes towards the animal pole to form a blastoderm, which is called a cell stage. Over time, the cells divide into 2, 4, 8, 16, 32, 64, 128 … … cells, which are more numerous and finer, and thicken from a single layer to multiple layers, which are morous, and are called morous stage. The first stage is about 4 hours at a water temperature of 25 ℃.
The blastocyst reaches the gastrulation stage. On the basis of the first cleavage stage, cell division is smaller, cell boundaries are difficult to see, and the number of cell layers is superposed to form a peak on the yolk, which is called high blastocyst stage. The cell area is then enlarged, 1/2 of the embryo body is continuously wrapped under the plant polar yolk, and the cell layer number is reduced, so the low blastocyst stage is called. Thereafter, the cell divides and the back lip appears with involution, also known as the embryonic shield. The embryonic shield appears in the early stage called primitive gut. The lower bag 2/3 is called the midgut phase, which is the time for calculating the fertilization rate, and it takes about 8 hours from fertilization to midgut phase at a water temperature of about 25 ℃. When 3/4 is placed in the lower bag, it is called the late phase of the original intestine. This stage takes about 11 hours.
Stage of neuroembryonic stage. After the prointestinal phase, the entire yolk is enveloped, and the embryo body elongates, forming the central axis organs (neural tube, spinal cord and somites), called the neublastic phase. The embryo body is in a C shape, lies on the yolk, and 9 pairs of above body segments and paired optic bubbles can be seen through microscopic examination. It took about 15 hours to reach this stage (about 25 ℃).
The tail bud reaches the membrane emergence stage. After the nerve embryo, an enlarged and slightly protruded cell mass is formed on the ventral surface of the rear end of the embryo body, and is called tail bud, namely the tail bud stage. The division ability of the tail bud cell is strong, so that the embryo body continuously extends forwards and backwards, the body segment continuously increases, then the tail fin appears, the muscle effect appears, the heart beats, the muscle contraction and the diastolic frequency continuously increases, the embryo body continuously rotates in the egg membrane, the head capsule skin cell of the embryo body secretes hatching enzyme continuously increases, the egg membrane becomes thin, the elasticity is reduced, and the embryo body rotates to break the membrane, so the membrane period is called. It took about 36 hours to this stage (about 25 ℃).
And (5) seedling stage. The embryo is called post-embryonic development after the embryo emerges from the membrane, and some organs inside and outside the embryo are not formed yet. After the membrane emerges, the development and growth continue, the pectoral fin appears, the body color is formed, the air bladder is formed, the intestinal tract is formed, and the yolk sac gradually shrinks, disappears and is opened. At this time, the nutrition must be taken from the extracorporeal ingestion. The whole process (about 25 ℃) of this stage takes about 130 hours.
The method is based on the collection of ocean or fresh water drifting fish eggs, the collected fish eggs are incubated and developed in an artificial breeding mode, a high-resolution microscope is used for shooting morphological development characteristics of the fish eggs at regular intervals in a sampling mode, the number of the survival fish eggs in the sampled individuals is recorded, and finally the survival rate of the fish eggs at different development stages of the fish eggs is obtained, so that the total survival rate of the fish eggs is calculated. The defect that the survival rate of the fish eggs is accurate and difficult to estimate in the past is overcome, and the method has the advantages of rapidness, accuracy and high efficiency.
Drawings
FIG. 1 is a diagram illustrating the development of the present invention using black sea bream eggs as an example;
FIG. 2 is a development diagram of the present invention using the eggs of the fishes with bilateral frame spines as an example.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The present invention will be further described below, and it should be noted that the embodiments are based on the technical solution, and detailed implementation manners and specific operation procedures are given, but the protection scope of the present invention is not limited to the embodiments.
The invention discloses a method for calculating the survival rate of driftage roes according to the survival rates of the roes at different stages of development, which comprises the following steps:
s1, collecting a drifting roe sample;
s2, culturing the collected sample with roe in an artificial culture mode;
s3 separating different kinds of roe individuals and culturing respectively;
s4, manually sampling the separated cultured and developed fish eggs;
s5, observing the development stage of the roe embryo of the sampled roe under a microscope, and recording the survival rate of the roe;
s6, continuously sampling and observing and recording the development period and survival rate of the roe at each development stage;
s7, the survival rates of different development stages of the roe are multiplied to obtain the total survival rate of the roe.
In step S1, the driftage roe sample is marine driftage roe and/or roe and larval fish of inland river; wherein the collection of the ocean drifting fish roe adopts a large-scale plankton made of a silk screen with the aperture of 0.505mm to carry out surface layer horizontal trawl; the method comprises the steps of collecting fish eggs and fish larvae of inland rivers by using a conical net for quantitative collection, and simultaneously using a jiang net for auxiliary qualitative collection.
The artificial culture mode is to temporarily culture the fish eggs collected in the sea or fresh water field in a plastic bucket containing seawater or fresh water, and bring the fish eggs back to a laboratory on the same day for indoor incubation.
The separation of the different kinds of roe individuals is to distinguish the different kinds of roe individuals by utilizing the various quantifiability characters, countable characters and description characters of the different kinds of roe.
It should be noted that quantifiable properties often occur as absolute values or as ratios of lengths of different sites. In the fertilized egg and embryo development stage, the common measurement items include the yolk diameter, the egg membrane diameter, the oil globule diameter, the long diameter and the short diameter of the non-circular egg, the length of the egg membrane silk with the egg membrane silk type, and the like. In the formation of embryo, the length of the embryo in different developmental stages is also one of the bases for identifying the species. The ratio of the size of the eye to the length of the embryo body after the eye is formed; when the tail portion leaves the yolk sac, the ratio of the length of the tail portion to the length of the embryo body is different from species to species. After the embryo body is filmed, a certain morphological structure is formed along with the development of the fish body, and the measurable character is correspondingly increased. In the early development stage of embryoid body, the important characteristics commonly used include the diameter of the egg membrane and the size of the perivitelline space, and the late embryonic stage is the curling degree of the pre-membrane embryo in the egg membrane. The characteristics of the size of the young fish eyes after the film is produced, the ratio of the body height (body width) to the body length, the length of the tail part and the like have higher reference values.
The countable traits include various characteristics that can be directly counted in the early development stage of fish, such as the existence of fertilized eggs and oil globules in the embryo stage, such as the number, the number of muscle nodes, and the like, and the number of muscle nodes of the larval fish after the larval fish is born. In the fertilized egg and embryo stage, the existence and number of oil globules and the existence of ootheca silk have great difference among different species, and can be used for distinguishing different species.
Descriptive traits are a class of comprehensive features. These features are often difficult to represent in a quantitative manner and appear in a textual narrative. Such as the shape and structure of the egg, the color of the yolk, the color of the oil globule, the order of appearance of organs during embryonic development, the body shape and pigment distribution of the fry, the size and body shape of the eye, etc.
In step S5, the survival rate of the roe embryo development is calculated by recording the total number of the sampled roe at this stage and observing the number of the surviving individuals through microscopic examination, thereby obtaining the survival rate of the roe embryo development at this stage.
It should be further noted that in step S5 of the present invention, the development process of the fish egg embryo is roughly divided into five stages. The five stages can be clearly observed and divided through microscopic examination, can also be roughly observed by naked eyes, and can improve the accuracy of observation by combining the time periods of embryo development under different water temperature conditions. Specifically, the method comprises the following steps:
and (4) a cleavage stage. After fertilization of fish eggs, the egg membrane begins to absorb water and swell, and meanwhile cytoplasm gradually concentrates and protrudes towards the animal pole to form a blastoderm, which is called a cell stage. Over time, the cells divide into 2, 4, 8, 16, 32, 64, 128 … … cells, which are more numerous and finer, and thicken from a single layer to multiple layers, which are morous, and are called morous stage. The first stage is about 4 hours at a water temperature of 25 ℃.
The blastocyst is in the gastral stage. On the basis of the first cleavage stage, cell division is smaller, cell boundaries are difficult to see, and the number of cell layers is superposed to form a peak on the yolk, which is called high blastocyst stage. The cell area is then enlarged, 1/2 of the embryo body is continuously wrapped under the plant polar yolk, and the cell layer number is reduced, so the low blastocyst stage is called. Thereafter, the cell divides and the back lip appears with involution, also known as the embryonic shield. The embryonic shield appears in the early stage called primitive gut. The lower bag 2/3 is called the midgut phase, which is the time for calculating the fertilization rate, and it takes about 8 hours from fertilization to midgut phase at a water temperature of about 25 ℃. When 3/4 is placed in the lower bag, it is called the late phase of the original intestine. This stage takes about 11 hours.
Stage of neuroembryonic stage. After the prointestinal phase, the entire yolk is enveloped, and the embryo body elongates, forming the central axis organs (neural tube, spinal cord and somites), called the neublastic phase. The embryo body is in a C shape, lies on the yolk, and 9 pairs of above body segments and paired optic bubbles can be seen through microscopic examination. It took about 15 hours to reach this stage (about 25 ℃).
The tail bud reaches the membrane emergence stage. After the nerve embryo, an enlarged and slightly protruded cell mass is formed on the ventral surface of the rear end of the embryo body, and is called tail bud, namely the tail bud stage. The division ability of the tail bud cell is strong, the embryo body continuously extends forwards and backwards, the body section is continuously increased, then the tail fin appears, the muscle effect appears, the heart beats, the muscle contraction and the diastolic frequency are continuously increased, the embryo body continuously rotates in the egg membrane, the hatching enzyme secretion of the head capsule skin cell of the embryo body is continuously increased, the egg membrane becomes thin, the elasticity is reduced, and the embryo body rotates to break the membrane, so the membrane stage is called. It took about 36 hours to this stage (about 25 ℃).
And (5) seedling stage. The embryo is called post-embryonic development after the embryo emerges from the membrane, and some organs inside and outside the embryo are not formed yet. After the membrane emerges, the development and growth continue, the pectoral fin appears, the body color is formed, the air bladder is formed, the intestinal tract is formed, and the yolk sac gradually shrinks, disappears and is opened. At this time, the nutrition must be taken from the extracorporeal ingestion. The whole process of the stage (about 25 ℃) takes about 130 hours.
Example one
As shown in fig. 1, the total survival rate of the black porgy roe is statistically calculated by taking the survival rate of the black porgy roe as an example, but it should be noted that the black porgy roe used in the embodiment of the present invention should not be construed as a limitation to the present invention, because the present invention provides a method for calculating the total survival rate of the roe.
(1) Collecting drifting roe sample, collecting marine roe according to ocean survey Specification (GB 12763.1-7-91), and making into large plankton net (with inner diameter of net mouth of 80cm, length of 270cm, and area of net mouth of 0.5 m) with screen having pore diameter of 0.505mm 2 ) Carrying out surface layer horizontal trawling according to preset survey station sites; collecting fish eggs of inland rivers by using a Bongo net simulated conical net for quantitative collection, and meanwhile, performing auxiliary qualitative collection by using a jiang net modified according to a fisherman boom net;
(2) temporarily breeding the collected roes in a plastic bucket containing seawater or fresh water, and taking the roes back to a laboratory on the same day for indoor incubation.
(3) Distinguishing different types of roes by using the difference of various quantifiable characters, countable characters and description characters of the roes of different types, and then respectively culturing;
(4) artificially sampling the separated black porgy roes cultured and developed;
(5) placing the sampled black porgy roes under a microscope to observe the development period of roe embryos, recording the development period of the roes according to the characteristics of the development period of the black porgy roes embryos, simultaneously recording the survival number of the sampled roes, and dividing the survival number by the total sampling number to calculate the survival rate of the roes at the period;
(6) continuously sampling the roes, observing and recording the development period of the roes in a microscope, and calculating the survival rate of the roes at the development period;
(7) the survival rate of the roe at each development stage is obtained by sampling, observing and recording the development stage and the survival rate of the roe for a plurality of times, and the survival rates at different development stages are multiplied to obtain the total survival rate of the roe;
(8) and (4) repeating the steps (4) to (7) on the respectively cultured roe samples, so that the survival rate of various roes can be calculated.
(9) The survival rate of the black porgy roe at the 2-cell stage is 100.0 percent; the survival rate of the fish eggs in the 4-cell stage is 100.0 percent; the survival rate of the fish eggs in the 8-64 cell stage is 99.8 percent; the survival rate of the roe in the multicellular stage is 100.0 percent; the survival rate of the fish eggs in the mulberry stage-high blastocyst stage is 100.0 percent; the survival rate of the fish eggs in the low blastocyst stage-early gastrula stage is 97.7 percent; the survival rate of roe in the middle-late primitive intestine is 99.9%; the survival rate of the fish eggs in the embryo forming stage-embryo hole closing stage is 99.0 percent; the survival rate of the fish eggs in the visual capsule forming period-sarcomere emergence period is 100.0 percent; the survival rate of the fish eggs in the auditory capsule forming period-cerebral vacuole forming period is 100.0 percent; the survival rate of the roe in the heart forming period is 99.7 percent; the survival rate of the fish eggs at the tail bud stage is 100.0 percent; the survival rate of the fish eggs in the incubation period is 99.8 percent; the survival rate of the fish eggs in the hatching period is 99.9 percent.
(10) The total survival rate of the black porgy roe is as follows:
1.000*1.000*0.998*1.000*1.000*0.977*0.999*0.990*1.000*1.000*0.997*1.000*0.998*0.999＝0.959＝95.9％。
| developmental stage of roe | Survival rate of black porgy/%) | Survival rate/100 |
| Stage 2 cells | 100.0 | 1.000 |
| 4 cell stage | 100.0 | 1.000 |
| 8-64 cell stage | 99.8 | 0.998 |
| Multiple cell stage | 100.0 | 1.000 |
| Mulberry stage-high blastocyst stage | 100.0 | 1.000 |
| Low blastocyst stage-early gastrulation stage | 97.7 | 0.977 |
| Mid-primitive gut-late primitive gut | 99.9 | 0.999 |
| Embryo body formation stage-pore sealing stage | 99.0 | 0.990 |
| Optic capsule formation phase-sarcomere emergence phase | 100.0 | 1.000 |
| Auditory capsule formation phase-cerebral vacuole formation phase | 100.0 | 1.000 |
| In the stage of cardiac formation | 99.7 | 0.997 |
| Stage of tail bud | 100.0 | 1.000 |
| Will hatch | 99.8 | 0.998 |
| Incubation period | 99.9 | 0.999 |
| Overall survival rate | 0.959 |
Example two
As shown in fig. 2, the present invention takes the survival rate of the eggs of the fish with bilateral frame spines as an example, and calculates the total survival rate of the eggs of the fish with bilateral frame spines statistically. It should be noted that the method of implementation is identical to that of implementation and therefore is not further stated.
The following table shows the survival rates of the eggs of the thorns and bilateral fishes at different development stages:
| developmental stage of roe | The egg/content of Amaranthus japonicus | Survival rate of 100 |
| Stage 2 cells | 100.0 | 1.000 |
| 4 cell stage | 100.0 | 1.000 |
| 8-64 cell stage | 99.7 | 0.997 |
| Multiple cell stage | 99.6 | 0.996 |
| Mulberry stage-high blastocyst stage | 100.0 | 1.000 |
| Low blastocyst stage-early gastrula stage | 99.0 | 0.990 |
| Mid-primitive gut-late primitive gut | 99.99 | 0.9999 |
| Embryo body formation phase-pore sealing phase | 100.0 | 1.000 |
| Optic capsule formation phase-sarcomere appearance phase | 100.0 | 1.000 |
| Auditory capsule formation phase-cerebral vacuole formation phase | 99.9 | 0.999 |
| In the stage of cardiac formation | 100.0 | 1.000 |
| Stage of tail bud | 100.0 | 1.000 |
| Will hatch | 100.0 | 1.000 |
| Incubation period | 99.99 | 0.9999 |
| Overall survival rate | 0.982 |
Various modifications may be made by those skilled in the art based on the foregoing technical solutions and concepts, and all such modifications should be included in the scope of the present invention.
Claims (5)
1. The method for calculating the survival rate of the drifting roe according to the survival rate of the roe at different stages of development is characterized by comprising the following steps:
s1, collecting a drifting roe sample;
s2, carrying out fish egg culture on the collected sample in an artificial culture mode;
s3 separating different kinds of roe individuals and culturing respectively;
s4, manually sampling the separated cultured and developed fish eggs;
s5, observing the development stage of the roe embryo of the sampled roe under a microscope, and recording the survival rate of the roe;
s6, continuously sampling and observing and recording the development stage and survival rate of the roe at each development stage;
s7, the survival rates of all the development stages of the roe are multiplied to obtain the total survival rate of the roe.
2. The method for calculating the survival rate of drifting fish eggs according to the survival rate of fish eggs at different stages of development according to the claim 1, wherein in the step S1, the drifting fish egg sample is the sea drifting fish egg and/or the fish egg of inland river; wherein, the collection of the ocean drifting fish eggs adopts a large-scale plankton sieve made of bolting silk with the aperture of 0.505mm, and then the collection of a horizontal trawl on the surface layer is carried out; the collection of the roe of inland river uses the circular cone net to carry out quantitative collection, uses jiang net to carry out supplementary qualitative collection simultaneously.
3. The method for calculating the survival rate of floating roes according to the survival rate of the roes at different stages of development according to claim 1, wherein the artificial culture method comprises temporarily culturing the roes collected in the ocean or fresh water field in a plastic bucket containing seawater or fresh water, and taking the roes back to the laboratory on the same day for indoor incubation.
4. The method for calculating the survival rate of drifted roe according to the survival rate of roe at different stages of development according to claim 1, wherein the separation of individual roe of different species is carried out by differentiating different species with different species of roe in various quantifiable traits, quantifiable traits and descriptive traits.
5. The method for calculating the survival rate of floating roe according to the survival rate of roe at different stages of development according to claim 1, wherein in step S5, the survival rate of roe at embryo development stage is calculated by recording the total number of roe sampled at the stage and observing the number of surviving individuals through microscopic examination, thereby obtaining the survival rate of roe at embryo development stage.
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