CN103583440A - Method for fast and physically distinguishing quality of mature ova of hucho taimen - Google Patents
Method for fast and physically distinguishing quality of mature ova of hucho taimen Download PDFInfo
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- CN103583440A CN103583440A CN201310589776.7A CN201310589776A CN103583440A CN 103583440 A CN103583440 A CN 103583440A CN 201310589776 A CN201310589776 A CN 201310589776A CN 103583440 A CN103583440 A CN 103583440A
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for fast and physically distinguishing the quality of mature ova of hucho taimen and relates to a physical evaluation method for the quality of the ova of hucho taimen. The method aims to solve the technical problems that in the existing multiplicative process of hucho taimen, the mature ova are evaluated by operators through intuitionistic senses from experience, the ova of hucho taimen cannot be evaluated at the early stage fast and accurately, consequently, the fertility rate and the hatchability decrease, and the membrane rupture offspring seed malformation rate and the floating offspring seed death rate increase. According to the method, the pubescent female parent fishes of hucho taimen are narcotized, the mature ova are extruded to a spawning basin containing an ovum buffer cleaning solution for trout from the abdominal cavities of the fish bodies, the average weight of the fish ova and the dispersion coefficient of the average weight, the average diameter of the fish ova and the dispersion coefficient of the average diameter and the average distribution density of fat droplets of the fish ova are measured, judgment is made on the basis of the physically-measured values to divide the fish ova into the superior grade, the medium grade and the inferior grade, the judgment can be completed in 1.5h, and the method has the advantage of being capable of being used for distinguishing the quality of the ova conveniently at the early stage. The method can be used in the field of aquaculture of hucho taimen.
Description
Technical field
The present invention relates to the physical evaluation method of Tai Menzheluo ovum quality.
Background technology
The Tai Menzheluo Gui Shu salmon Salmonisdae of section, wise sieve salmon belong to Hucho.Tai Menzheluo salmon is mainly distributed in Russia, China, Mongolia and Japanese.At present Zhe Luogui China is in imminent danger, Wusuli River only, and the Hanasi Lake, xinjiang Province in Heilungkiang and Xinjiang has a small amount of reproductive population existence.Tai Menzheluo salmon as the Typical Representative of China original inhabitants cold water fishes, no matter from plasm resource protection, or is considered from culture benefit, studies this fish and be all extremely important and social value.Unique large-scale fish in a few indigenous salmon fishes of Tai Menzheluo Gui Shi China, belong to rare famous and precious kind, Tai Menzheluo salmon artificial propagation success after deliberation, and realized the ingest scale domestication of artificial feed of prelarva.Tai Menzheluo salmon is promoted through 10 years of researches, at present this fish in Yunnan, the national 20Duo Ge provinces, cities and autonomous regions such as Sichuan, Heilungkiang, Liaoning, Jilin and Shandong have carried out promoting cultivation and enhancement releasing, are deeply subject to raiser and social welcome.In recent years along with Tai Menzheluo salmon, propagate the expansion of industry artificially, also in growth at double, there is the good and bad uneven phenomenon of mature egg in the demand of seed in actual production process.Because requiring brooding time under 4-8 ℃ of limpid flowing water environment, the cultivation of Tai Menzheluo salmon fertilized egg reaches more than 30 days, and very easily infect water mold (fungi a kind of) at hatching process, because fish-egg is mainly comprised of protein, and the protein good natural medium that is water mold, particularly dead or second-rate fertilized egg not only self is very easily infected water mold, and can be by the infection in blocks of good fish-egg, cause the fertility rate and hatchability of ovum to reduce greatly, indivedual hatching pail incubation rates are even less than 10%.Even if fertilized egg can be spent smoothly the hatching stage and be survived, but because the quality good or not of part ovum own differs greatly, cause lethality and abnormal rate after fry rupture of membranes also very high.Above problem, not only in breeding process, reduced production efficiency, simultaneously also significant wastage material and economic resources, serious restriction further developing of the germ plasm resource rejuvenation of Tai Menzheluo salmon and aquaculture industry, therefore, distinguishing in early days of the mature egg quality of Tai Menzheluo salmon becomes bottleneck problem urgently to be resolved hurrily.
Tai Menzheluo Gui China large-scale artificial development time is shorter, so the evaluation of the ovum of Tai Menzheluo salmon maturation there is no effective method at present, can only be by operating personnel, to be evaluated intuitively by rule of thumb in artificial propagation process.Due to the survival rate for assurance ovum, mature egg must complete artificial insemination after take out in parent population abdominal cavity in 2h, so in artificial propagation process, necessarily requires to complete distinguishing of ovum quality in finite time.Due to different with operating personnel's technical merit it is pressed for time, very large of the error of quality appraisement that causes ovum quality, also the eye rate of the sending out less than 10% that just causes current part Tai Menzheluo salmon mature egg, rupture of membranes seed abnormal rate is up to 20%, and weed survival rate less than 60% floats.
Summary of the invention
The present invention is that will to solve in existing Tai Menzheluo salmon reproductive process the evaluation method of ripe ovum be by operating personnel's sensation intuitively by rule of thumb, can not realize early stage, quick and accurate evaluation, thereby cause fertilization rate, incubation rate to reduce, the technical problem that rupture of membranes seed abnormal rate and floating seed lethality increase, and provide a kind of rapid physical to distinguish the method for Tai Menzheluo salmon mature egg quality.
A kind of rapid physical of the present invention distinguishes that the method for Tai Menzheluo salmon mature egg quality carries out according to the following steps:
One, salmon is joined to clean laying eggs in basin with ovum buffering cleaning fluid;
Two, by the female parent population anesthesia of sexually matured Tai Menzheluo salmon, mature egg is extruded into from fish body abdominal cavity and fills in the basin of laying eggs of salmon with ovum buffering cleaning fluid, the basin of laying eggs is only put the mature egg of a parent population, and the basin of then laying eggs is positioned over ventilation lucifugal place;
Three, from the basin of laying eggs is random, take out several fish-eggs, join in clear water after static 25～30min, measure weight and the diameter of simple grain fish-egg, then calculate the mean value X of simple grain fish-egg weight
1with standard deviation SV
1, the mean value X of simple grain fish-egg diameter
2with standard deviation SV
2; Press CV
1=(SV
1/ X
1the coefficient of dispersion CV of fish-egg weight is calculated in) * 100
1, by CV
2=(SV
2/ X
2the coefficient of dispersion CV of fish-egg diameter is calculated in) * 100
2;
Four, from several the fish-eggs of the random taking-up of basin of laying eggs, measure the fat drop distribution density of every fish-egg, and calculate the mean value X of fat drop distribution density
3;
If five satisfy condition 1. simultaneously, 2., 3., 4. and 5., the fish-egg of judging this parent population is top grade; 1. its conditional is 62.262mg≤X
1≤ 65.145mg; 2. condition is CV
1≤ 3.207; 3. condition is 5.610mm≤X
2≤ 5.878mm; 4. condition is CV
2≤ 2.948; 5. condition is 12.8/mm
2≤ X
3≤ 19.4/mm
2;
Six, do not meet top grade standard, but satisfy condition 6., 7., 8., 9. and 10. simultaneously, the fish-egg of judging this parent population is middle rank; 6. its conditional is 59.912mg≤X
1; 7. condition is CV
1≤ 4.461; 8. condition is 5.545mm≤X
2< 5.878mm; 9. condition is CV
2≤ 3.339; 10. condition is 9.7/mm
2≤ X
3≤ 22.6;
Seven, the fish-egg that does not meet intermediate standard is judged to be poor level; Complete distinguishing of Tai Menzheluo salmon mature egg quality.
The present invention adopts physical measurement and biostatistics technology to combine, measure weight, the diameter of ripe fish-egg, the physical indexs such as distribution density of fat drop, as the indirect indexes of evaluating ovum quality, but it has in early days, characteristic fast and easily, in the very short time, just can complete simultaneously, on next step processing of Tai Menzheluo salmon mature egg without any impact.Can to ovum quality, realize EARLY STAGE EVALUATION in Tai Menzheluo salmon mature egg prefecundation not yet, eliminate fast in advance ovum inferior.Take these data determines grade scale as basis, the fish-egg that retains top grade, carry out artificial culture, for intermediate ripe fish-egg, also retain and carry out artificial culture, as inadequate in cultivation hatching facility in production process, can mix cultivation with other intermediate ovum, but to firmly avoid and the cultivation that mixes of top grade ovum, effectively reduce the mutual infection of fungi in artificial propagation process, in order to avoid affect the effect of top grade ovum, and eliminate in time for the ripe fish-egg of poor level, need not proceed artificial culture, can be used as caviar and use.The top grade Tai Menzheluo salmon ovum of judging by this method, its fertilization rate is greater than 95%, send out that an eye rate is greater than 90%, abnormal rate is less than 1.5%, and the survival rate of floating seed is greater than 85%; The intermediate Tai Menzheluo salmon ovum of judging by the inventive method, its fertilization rate reached to 75%～95%, send out that an eye rate reaches 70%～90%, abnormal rate is 1.5%～2.4%, the survival rate of floating seed reaches 65%～85%; The poor level Tai Menzheluo salmon ovum of judging by method of the present invention, its fertilization rate is less than 75%, send out an eye rate reach be less than 70%, abnormal rate is greater than 2.4%, the survival rate of floating seed is less than 65%.Method of the present invention can be used for Tai Menzheluo salmon cultivation field.
Accompanying drawing explanation
Fig. 1 is fish-egg fat drop distribution density survey map in test 1;
Embodiment
Embodiment one: a kind of rapid physical of present embodiment distinguishes that the method for Tai Menzheluo salmon mature egg quality carries out according to the following steps:
One, salmon is joined to clean laying eggs in basin with ovum buffering cleaning fluid;
Two, by the female parent population anesthesia of sexually matured Tai Menzheluo salmon, mature egg is extruded into from fish body abdominal cavity and fills in the basin of laying eggs of salmon with ovum buffering cleaning fluid, the basin of laying eggs is only put the mature egg of a parent population, and the basin of then laying eggs is positioned over ventilation lucifugal place;
Three, from the basin of laying eggs is random, take out several fish-eggs, join in clear water after static 25～30min, measure weight and the diameter of simple grain fish-egg, then calculate the mean value X of simple grain fish-egg weight
1with standard deviation SV
1, the mean value X of simple grain fish-egg diameter
2with standard deviation SV
2; Press CV
1=(SV
1/ X
1the coefficient of dispersion CV of fish-egg weight is calculated in) * 100
1, by CV
2=(SV
2/ X
2the coefficient of dispersion CV of fish-egg diameter is calculated in) * 100
2;
Four, from several the fish-eggs of the random taking-up of basin of laying eggs, measure the fat drop distribution density of every fish-egg, and calculate the mean value X of fat drop distribution density
3;
If five satisfy condition 1. simultaneously, 2., 3., 4. and 5., the fish-egg of judging this parent population is top grade; 1. its conditional is 62.262mg≤X
1≤ 65.145mg; 2. condition is CV
1≤ 3.207; 3. condition is 5.610mm≤X
2≤ 5.878mm; 4. condition is CV
2≤ 2.948; 5. condition is 12.8/mm
2≤ X
3≤ 19.4/mm
2;
Six, do not meet top grade standard, but satisfy condition 6., 7., 8., 9. and 10. simultaneously, the fish-egg of judging this parent population is middle rank; 6. its conditional is 59.912mg≤X
1; 7. condition is CV
1≤ 4.461; 8. condition is 5.545mm≤X
2< 5.878mm; 9. condition is CV
2≤ 3.339; 10. condition is 9.7/mm
2≤ X
3≤ 22.6;
Seven, the fish-egg that does not meet intermediate standard is judged to be poor level; Complete distinguishing of Tai Menzheluo salmon mature egg quality.
Embodiment two: present embodiment is different from embodiment one: the salmon described in step 1 is prepared by the following method with ovum buffering cleaning fluid: add successively NaCl8.0g, KCl0.3g, CaCl in 1 liter of distilled water
20.3g, NaHCO
30.04g, is stirred to dissolving, obtains salmon and cushions cleaning fluid with ovum.Other is identical with embodiment one.
Embodiment three: present embodiment is different from embodiment one or two: simple grain fish-egg weight, fish-egg weighed average X
1measuring method as follows: in tested fish-egg, randomly draw fish-egg and form n group, every group of m grain ovum, blots the moisture of ovum band with filter paper, then the m grain ovum of i group is put on analytical balance and is measured, and the gross weight G of ovum respectively organized in record
i, then press simple grain fish-egg weight A
i=G
i/ m calculates the simple grain fish-egg weight A of i group
i; I=1 wherein, 2,3 ..., n; Simple grain fish-egg weighed average X
1=(A
1+ A
2+ ... + A
i+ ... + A
n)/n.Other are identical with embodiment one or two.
Embodiment four: that present embodiment is different from embodiment three is simple grain fish-egg weight, fish-egg weighed average X
1measuring method in n=25～30.Other is identical with embodiment three.
Embodiment five: that present embodiment is different from embodiment three or four is simple grain fish-egg weight, fish-egg weighed average X
1measuring method in m=5～8.Other is identical with embodiment three or four.
Embodiment six: present embodiment is different from one of embodiment one to five is that the measuring method of simple grain fish-egg diameter is as follows: randomly draw 25～30 fish-eggs, be placed on respectively with the minimum scale Steel Ruler that is 1mm on, with digital camera, take a picture, obtain image, the length X of fish-egg and the length Y of 1mm scale in image in measurement image, be that B=X/Y calculates by formula, obtains fish-egg diameter B, unit is mm, has completed the measurement of fish-egg diameter.Other is identical with one of embodiment one to five.
Embodiment seven: present embodiment is different from one of embodiment one to six is that the measuring method of distribution density of fat drop is as follows: with suction pipe, fish-egg is placed on slide, slide with ovum is placed under anatomical lens together, regulate up and down slide, make the top of ovum just in time under the object lens of anatomical lens, under micro imaging system, take one, the photo of ovum top, with micro-imaging software, in picture centre position, get the circle that area is Z, the unit of Z is mm
2, calculate the fat drop number B in circle, wherein the fat drop of the area of fat drop>=1/2 in circle added up according to 1 fat drop; Fat drop distribution density C=B/Z.Other is identical with one of embodiment one to six.
Embodiment eight: that present embodiment is different from embodiment seven is Z=10mm
2.Other is identical with embodiment seven.
Embodiment nine: present embodiment is different from one of embodiment one to eight is in step 3 to be 180～200 from a grain number for the random fish-egg taking out of basin of laying eggs.Other is identical with one of embodiment one to eight.
Embodiment ten: present embodiment is different from one of embodiment one to eight is in step 4 to be 30～40 from a grain number for the random fish-egg taking out of basin of laying eggs.Other is identical with one of embodiment one to eight.
With verification experimental verification beneficial effect of the present invention below:
Test 1, a kind of rapid physical of this test distinguish that the method for Tai Menzheluo salmon mature egg quality carries out according to the following steps:
One, salmon is joined to 6 clean laying eggs in basin with ovum buffering cleaning fluid; Described salmon is prepared by the following method with ovum buffering cleaning fluid:: in 1 liter of distilled water, add successively NaCl8.0g, KCl0.3g, CaCl
20.3g, NaHCO
30.04g, is stirred to dissolving, obtains salmon and cushions cleaning fluid with ovum; The basin number consecutively of laying eggs is 1#, 2#, 3#, 4#, 5#, 6#, 7#, 8#, 9#, 10#, 11# and 12#;
Two, by 12 female parent population anesthesia of sexually matured Tai Menzheluo salmon, respectively the mature egg of every parent population is extruded into from fish body abdominal cavity to the salmon of the basin of laying eggs with in ovum buffering cleaning fluid, every corresponding basin of laying eggs of parent population, the basin of then each being laid eggs is positioned over ventilation lucifugal place;
Three, from 1#, lay eggs that basin is random takes out 200 fish-eggs, join in clear water after static 30min, therefrom randomly draw again fish-egg and form 30 groups, every group of 5 fish-eggs, the moisture of ovum band is blotted with filter paper, then 5 ovums of the 1st group are put on analytical balance and measured, record the gross weight G of the 1st group of ovum
1, then press simple grain fish-egg weight A
1=G
1/ 5 calculate the simple grain fish-egg weight A of the 1st group
1; In like manner calculate the simple grain fish-egg weight A of all the other each groups
2, A
3..., A
30; Press X
1=(A
1+ A
2+ ... + A
30)/30 calculate simple grain fish-egg weighed average X
1; Calculate again the poor SV of simple grain fish-egg weight standard
1, then press CV
1=(SV
1/ X
1fish-egg weight coefficient of dispersion CV is calculated in) * 100
1; From laying eggs basin, 1# randomly draws again 30 fish-eggs, in the Steel Ruler that to be placed on respectively with minimum scale be 1mm, with digital camera, take a picture, obtain image, then use Computer Image Processing Survey Software, the length X of fish-egg and the length Y of 1mm scale in image in measurement image, by formula, be that B=X/Y calculates, obtain fish-egg diameter B, unit is mm, by the mean value X of each fish-egg diameter measuring
2; Then calculate standard deviation SV
2; Press CV
2=(SV
2/ X
2the coefficient of dispersion CV of fish-egg diameter is calculated in) * 100
2;
Four, from laying eggs basin, 1# takes out at random 30 fish-eggs, the measuring method of the distribution density of the fat drop of every fish-egg is as follows: with suction pipe, fish-egg is placed on slide, slide with ovum is placed under anatomical lens together, regulate up and down slide, make the top of ovum just in time under the object lens of anatomical lens, one, photo taking ovum top under micro imaging system, with micro-imaging software, getting area in picture centre position is 10mm
2circle, as shown in Figure 1, calculate the fat drop number B in circle, wherein the fat drop of the area of fat drop>=1/2 in circle added up according to 1 fat drop; By fat drop distribution density C=B/10, calculate this fish-egg fat drop distribution density C; And calculate the mean value X of the fat drop distribution density of these 30 fish-eggs
3;
In like manner by step 3 and four, measure the lay eggs X of fish-egg in basin of 2#, 3#, 4#, 5#, 6#, 7#, 8#, 9#, 10#, 11# and 12#
1, CV
1, X
2, CV
2and X
3value, result is as shown in table 1;
The measurement result of table 1 parent population fish-egg
| The basin of laying eggs | X 1（mg） | CV 1 | X 2（mm） | CV 2 | X 3(individual/mm 2） |
| 1＃ | 64.214 | 2.981 | 5.756 | 2.664 | 18.3 |
| 2＃ | 60.423 | 3.595 | 5.545 | 3.129 | 20.7 |
| 3＃ | 63.189 | 3.105 | 5.768 | 2.867 | 11.3 |
| 4＃ | 60.198 | 3.028 | 5.624 | 2.921 | 18.6 |
| 5＃ | 64.001 | 3.015 | 5.885 | 2.967 | 13.9 |
| 6＃ | 50.471 | 4.125 | 4.312 | 2.998 | 21.8 |
| 7＃ | 62.498 | 2.654 | 5.731 | 2.495 | 16.1 |
| 8＃ | 65.145 | 2.338 | 5.820 | 2.902 | 19.1 |
| 9＃ | 66.154 | 3.762 | 5.854 | 3.214 | 21.9 |
| 10# | 54.912 | 6.889 | 0.544 | 5.515 | 17.3 |
| 11# | 60.513 | 4.687 | 0.534 | 4.841 | 23.2 |
| 12# | 69.181 | 3.203 | 5.582 | 3.251 | 21.3 |
If five satisfy condition 1. simultaneously, 2., 3., 4. and 5., the fish-egg of judging this parent population is top grade; 1. its conditional is 62.262mg≤X
1≤ 65.145mg; 2. condition is CV
1≤ 3.207; 3. condition is 5.610mm≤X
2≤ 5.878mm; 4. condition is CV
2≤ 2.948; 5. condition is 12.8/mm
2≤ X
3≤ 19.4/mm
2;
Six, do not meet top grade standard, but satisfy condition 6., 7., 8., 9. and 10. simultaneously, the fish-egg of judging this parent population is middle rank; 6. its conditional is 59.912mg≤X
1; 7. condition is CV
1≤ 4.461; 8. condition is 5.545mm≤X
2< 5.878mm; 9. condition is CV
2≤ 3.339; 10. condition is 9.7/mm
2≤ X
3≤ 22.6;
Seven, the fish-egg that does not meet intermediate standard is judged to be poor level; Complete distinguishing of Tai Menzheluo salmon mature egg quality.
Good and bad result after distinguishing according to step 5, six and seven is as shown in table 2,
The good and bad result of table 2 parent population fish-egg
| The basin of laying eggs | 1＃ | 2＃ | 3＃ | 4＃ | 5＃ | 6＃ |
| Rank | Top grade | Middle rank | Middle rank | Middle rank | Middle rank | Poor level |
| The basin of laying eggs | 7＃ | 8＃ | 9＃ | 10＃ | 11＃ | 12＃ |
| Rank | Top grade | Top grade | Middle rank | Poor level | Poor level | Middle rank |
From 1#, 2#, 3#, 4#, 5#, 6#, 7#, 8#, 9#, 10#, 11# and 12# are laid eggs basin, each takes out respectively 5000 fish-eggs at random, after artificial insemination, being placed on temperature is to hatch separately under 4～8 ℃ of limpid flowing water environment, it is incubator box of fish-egg of every fish, after hatching 10 days, measure the fertilization rate of fish-egg, after hatching 22 days, measure the eye rate of sending out of fish-egg, after the whole ruptures of membranes of fertilized egg, add up abnormal rate, the survival rate of statistics floating seed after fry floating.It is as shown in table 3 that the aquaculture organism obtaining is learned proterties specific targets result,
Table 3 aquaculture organism is learned proterties specific targets result
| The basin of laying eggs | Fertilization rate | Send out eye rate | Abnormal rate | Survival rate |
| 1＃ | 98% | 93.2% | 0.8% | 89.2% |
| 2＃ | 88.3% | 81.2% | 1.7% | 79.3% |
| 3＃ | 93.5% | 85.1% | 1.9% | 83.6% |
| 4＃ | 83.7% | 80.0% | 1.7% | 70.4% |
| 5＃ | 78.8% | 76.3% | 1.6% | 68.8% |
| 6＃ | 62.1% | 48.5% | 12.4% | 50.6% |
| 7＃ | 96.2% | 91.4% | 0.9% | 89.7% |
| 8# | 98.4% | 92.7% | 0.7% | 90.7% |
| 9# | 85.6% | 79.9% | 2.1% | 77.3% |
| 10# | 53.4% | 32.1% | 8.5% | 23.5% |
| 11# | 67.5% | 56.6% | 7.9% | 40.2% |
| 12# | 91.3% | 73.8% | 2.3% | 71.3% |
It is mould that the fish-egg that the 6# of this test is laid eggs in basin has infected Shui at hatching process, and water mold causes the fertility rate and hatchability of ovum to reduce greatly.From then on aquaculture organism is learned the concrete test index of proterties, and this test is believable for the evaluation method of fish-egg.This test adopts physical measurement and biostatistics technology to combine, measure weight, the diameter of ripe fish-egg, the physical indexs such as distribution density of fat drop, as the indirect indexes of evaluating ovum quality, there is characteristic in early days, fast and easily, within the time of 1.5h, just can complete simultaneously, on next step processing of Tai Menzheluo salmon mature egg without any impact.Can, in Tai Menzheluo salmon mature egg prefecundation not yet, to ovum quality, realize EARLY STAGE EVALUATION.
Claims (10)
1. rapid physical is distinguished a method for Tai Menzheluo salmon mature egg quality, it is characterized in that carrying out according to the following steps:
One, salmon is joined to clean laying eggs in basin with ovum buffering cleaning fluid;
Two, by the female parent population anesthesia of sexually matured Tai Menzheluo salmon, mature egg is extruded into from fish body abdominal cavity and fills in the basin of laying eggs of salmon with ovum buffering cleaning fluid, the basin of laying eggs is only put the mature egg of a parent population, and the basin of then laying eggs is positioned over ventilation lucifugal place;
Three, from the basin of laying eggs is random, take out several fish-eggs, join in clear water after static 25～30min, measure weight and the diameter of simple grain fish-egg, then calculate the mean value X of simple grain fish-egg weight
1with standard deviation SV
1, the mean value X of simple grain fish-egg diameter
2with standard deviation SV
2; Press CV
1=(SV
1/ X
1the coefficient of dispersion CV of fish-egg weight is calculated in) * 100
1, by CV
2=(SV
2/ X
2the coefficient of dispersion CV of fish-egg diameter is calculated in) * 100
2;
Four, from several the fish-eggs of the random taking-up of basin of laying eggs, measure the fat drop distribution density of every fish-egg, and calculate the mean value X of fat drop distribution density
3;
If five satisfy condition 1. simultaneously, 2., 3., 4. and 5., the fish-egg of judging this parent population is top grade; 1. its conditional is 62.262mg≤X
1≤ 65.145mg; 2. condition is CV
1≤ 3.207; 3. condition is 5.610mm≤X
2≤ 5.878mm; 4. condition is CV
2≤ 2.948; 5. condition is 12.8/mm
2≤ X
3≤ 19.4/mm
2;
Six, do not meet top grade standard, but satisfy condition 6., 7., 8., 9. and 10. simultaneously, the fish-egg of judging this parent population is middle rank; 6. its conditional is 59.912mg≤X
1; 7. condition is CV
1≤ 4.461; 8. condition is 5.545mm≤X
2< 5.878mm; 9. condition is CV
2≤ 3.339; 10. condition is 9.7/mm
2≤ X
3≤ 22.6;
Seven, the fish-egg that does not meet intermediate standard is judged to be poor level; Complete distinguishing of Tai Menzheluo salmon mature egg quality.
2. a kind of rapid physical according to claim 1 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that the salmon described in step 1 prepares by the following method with ovum buffering cleaning fluid: in 1 liter of distilled water, add successively NaCl8.0g, KCl0.3g, CaCl
20.3g, NaHCO
30.04g, is stirred to dissolving, obtains salmon and cushions cleaning fluid with ovum.
3. a kind of rapid physical according to claim 1 and 2 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that simple grain fish-egg weight in step 3, fish-egg weighed average X
1measuring method as follows: in tested fish-egg, randomly draw fish-egg and form n group, every group of m grain ovum, blots the moisture of ovum band with filter paper, then the m grain ovum of i group is put on analytical balance and is measured, and the gross weight G of ovum respectively organized in record
i, then press simple grain fish-egg weight A
i=G
i/ m calculates the simple grain fish-egg weight A of i group
i; I=1 wherein, 2,3 ..., n; Simple grain fish-egg weighed average X
1=(A
1+ A
2+ ... + A
i+ ... + A
n)/n.
4. a kind of rapid physical according to claim 3 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that simple grain fish-egg weight, fish-egg weighed average X
1measuring method in n=25～30.
5. a kind of rapid physical according to claim 3 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that simple grain fish-egg weight, fish-egg weighed average X
1measuring method in m=5～8.
6. a kind of rapid physical according to claim 1 and 2 is distinguished the method for Tai Menzheluo salmon mature egg quality, the measuring method that it is characterized in that simple grain fish-egg diameter is as follows: randomly draw 25～30 fish-eggs, be placed on respectively with the minimum scale Steel Ruler that is 1mm on, with digital camera, take a picture, obtain image, the length X of fish-egg and the length Y of 1mm scale in image in measurement image, by formula, be that B=X/Y calculates, obtain fish-egg diameter B, unit is mm, has completed the measurement of fish-egg diameter.
7. a kind of rapid physical according to claim 1 and 2 is distinguished the method for Tai Menzheluo salmon mature egg quality, the measuring method that it is characterized in that the distribution density of fat drop in step 4 is as follows: with suction pipe, fish-egg is placed on slide, slide with ovum is placed under anatomical lens together, regulate up and down slide, make the top of ovum just in time under the object lens of anatomical lens, under micro imaging system, take one, the photo of ovum top, with micro-imaging software, in picture centre position, get the circle that area is Z, the unit of Z is mm
2, calculate the fat drop number B in circle, wherein the fat drop of the area of fat drop>=1/2 in circle added up according to 1 fat drop; Fat drop distribution density C=B/Z.
8. a kind of rapid physical according to claim 7 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that Z=10mm in the measuring method of distribution density of fat drop
2.
9. a kind of rapid physical according to claim 1 and 2 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that in step 3 being 180～200 from the grain number of the random fish-egg taking out of basin of laying eggs.
10. a kind of rapid physical according to claim 1 and 2 is distinguished the method for Tai Menzheluo salmon mature egg quality, it is characterized in that in step 4 being 30～40 from the grain number of the random fish-egg taking out of basin of laying eggs.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296337A (en) * | 2020-03-24 | 2020-06-19 | 上海海洋大学 | Method for rapidly identifying ovum maturity of hyriopsis cumingii without damage |
| CN111655030A (en) * | 2017-12-01 | 2020-09-11 | 斯托芬菲库有限责任公司 | Aquaculture method for producing salmon roe |
| CN112384768A (en) * | 2018-05-04 | 2021-02-19 | 艾克斯波特西溶液公司 | Scale for determining the weight of a biological body |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63282653A (en) * | 1987-05-14 | 1988-11-18 | Kachiku Jiyuseiran Ishiyoku Gijutsu Kenkyu Kumiai | Method for measuring activity of cell |
| JPH09304284A (en) * | 1996-05-20 | 1997-11-28 | Kawatetsu Joho Syst Kk | Image processing method for inspecting egg |
| JP2006109918A (en) * | 2004-10-12 | 2006-04-27 | Nagasaki Prefecture | Method for stably and efficiently collecting egg of fish using egg collection result prediction data based on egg diameter |
| CN1825158A (en) * | 2005-02-25 | 2006-08-30 | 宝钢集团上海梅山有限公司 | Digital metallurgical microscopic image enlarging multiplying power calibrating method |
| CN103039392A (en) * | 2013-01-17 | 2013-04-17 | 中国水产科学研究院黑龙江水产研究所 | Living body continuous measurement method of morphological traits of cold water roes or fry |
-
2013
- 2013-11-21 CN CN201310589776.7A patent/CN103583440B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63282653A (en) * | 1987-05-14 | 1988-11-18 | Kachiku Jiyuseiran Ishiyoku Gijutsu Kenkyu Kumiai | Method for measuring activity of cell |
| JPH09304284A (en) * | 1996-05-20 | 1997-11-28 | Kawatetsu Joho Syst Kk | Image processing method for inspecting egg |
| JP2006109918A (en) * | 2004-10-12 | 2006-04-27 | Nagasaki Prefecture | Method for stably and efficiently collecting egg of fish using egg collection result prediction data based on egg diameter |
| CN1825158A (en) * | 2005-02-25 | 2006-08-30 | 宝钢集团上海梅山有限公司 | Digital metallurgical microscopic image enlarging multiplying power calibrating method |
| CN103039392A (en) * | 2013-01-17 | 2013-04-17 | 中国水产科学研究院黑龙江水产研究所 | Living body continuous measurement method of morphological traits of cold water roes or fry |
Non-Patent Citations (4)
| Title |
|---|
| 徐伟等: ""鱼类科研工作中几个问题的解决技巧"", 《水产学杂志》, vol. 20, no. 1, 31 May 2007 (2007-05-31), pages 107 - 111 * |
| 李雪: ""雌性哲罗鲑繁殖性能的研究"", 《中国优秀硕士学位论文农业科技辑》, 31 March 2013 (2013-03-31) * |
| 白鸿钧: "《统计学原理》", 30 June 2004, article ""统计分布的数值特征"", pages: 129 * |
| 贾玉东等: ""硬骨鱼类卵子质量研究进展"", 《中国水产科学》, vol. 19, no. 3, 31 May 2012 (2012-05-31), pages 545 - 555 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111655030A (en) * | 2017-12-01 | 2020-09-11 | 斯托芬菲库有限责任公司 | Aquaculture method for producing salmon roe |
| US11310999B2 (en) | 2017-12-01 | 2022-04-26 | Stofnfiskur Hf | Aquaculture process for the production of salmon eggs |
| CN111655030B (en) * | 2017-12-01 | 2022-08-12 | 斯托芬菲库有限责任公司 | Aquaculture method for producing salmon roe |
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| CN111296337A (en) * | 2020-03-24 | 2020-06-19 | 上海海洋大学 | Method for rapidly identifying ovum maturity of hyriopsis cumingii without damage |
| CN113498748A (en) * | 2021-06-04 | 2021-10-15 | 广东海洋大学 | Method for calculating survival rate of drifting roes according to survival rates of roes at different stages of development |
| CN113498748B (en) * | 2021-06-04 | 2022-09-27 | 广东海洋大学 | Method for calculating survival rate of drifting roes according to survival rates of roes at different stages of development |
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