CN103039392A - Living body continuous measurement method of morphological traits of cold water roes or fry - Google Patents
Living body continuous measurement method of morphological traits of cold water roes or fry Download PDFInfo
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- CN103039392A CN103039392A CN201310017656XA CN201310017656A CN103039392A CN 103039392 A CN103039392 A CN 103039392A CN 201310017656X A CN201310017656X A CN 201310017656XA CN 201310017656 A CN201310017656 A CN 201310017656A CN 103039392 A CN103039392 A CN 103039392A
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Abstract
The invention discloses a living body continuous measurement method of morphological traits of cold water roes or fry, relates to the field of living body continuous measurement methods of the roes or the fry and aims to overcome the defects that measurement errors are large, the roes or the fry are/is prone to deform and die, and the difficulty is high in the continuous measurement study process in terms of existing measurement methods of the roes or the fry. The method includes that (1) a measuring tank is prepared and water is filled in the measuring tank; (2) the roes or the fry are/is transferred into the measuring tank; (3) the roes or the fry are/is subjected to image acquisition; (4) the roes or the fry are/is put back; and (5) image data are subjected to analysis and measurement by software, calculation is performed by means of a scaling formula to obtain actual morphological data of the roes or the fry, and namely the living body continuous measurement of the morphological traits of the cold water roes or fry is completed. The method is suitable for the field of cold water fish breeding industries.
Description
Technical field
The present invention relates to the field of fish-egg or seed live body method for continuous measuring.
Background technology
Cold water fishes have fine and tender taste, nutritious, edible part large, without thorn between flesh, be easy to the characteristics such as processing, the most important thing is that protein content is high in the muscle, and contain a large amount of unsaturated fatty acids, along with China's expanding economy, people's daily life level improves constantly, the traditional fish species such as green grass or young crops, grass, silver carp, flathead is can not satisfying people's consumer need fully, so the high-quality protein fish culture of China's cold water already develops very rapid.Present cold water fishes has become China and even in the world one of important high-quality protein and source of nutriment.In recent years along with country and local government to the great attention of cold water fish aquaculture, cultured area is continuing to increase, China to the research input of cold water fishes seed also in continuous increase.
Because wise sieve, rainbow trout, with the cold water fish fertilized egg such as white point salmon and Larval nature physilogical characteristics, all has long feature of incubating oosperm and Larval time, indivedual kinds reached more than 3 months, therefore extremely difficult in the research process of continuity measurement cold water fishes early growth and development, traditional fertilized egg and the measuring method of prelarva all are to adopt vernier calliper to measure manually, owing to measure manually slower, it is longer to require like this fertilized egg to be exposed in the air time, simultaneously also very easily bring physical damnification to fertilized egg, greatly increase the lethality of test sample.The measuring method of traditional prelarva, all need to anaesthetize rear measurement, owing to the phenomenon that overdose causes prelarva death often occurs in the narcotic use procedure, even the prelarva of survival after the anesthesia is owing to be in the body structure Differentiation and development stage, narcotic use tends to cause a large amount of Larval deformities.Traditional measuring method all is to measure manually, because fertilized egg and prelarva generally less (minimum 3 millimeters), the result of measurement often error is larger.
Summary of the invention
The present invention is the measuring method that will solve existing fish-egg or seed, has large, the easy deformity and dead of measure error, the high shortcoming of difficulty in the continuous measurement research process, and the live body method for continuous measuring of a kind of cold water fish ovum or seed morphological characters is provided.
The live body method for continuous measuring of a kind of cold water fish ovum or seed morphological characters, specifically finish according to the following steps:
One, prepares measuring flume, injected water in the measuring flume; Wherein, minimum scale is arranged is the scale of 1mm in the bottom of described measuring flume;
Two, fish-egg or seed are transferred to from culture pond in the measuring flume of the water filling that step 1 obtains;
Three, adopt image capture device perpendicular to the water surface in the measuring flume of step 2 the fish-egg in the measuring flume or seed to be carried out acquisition of image data; Wherein, the zone of collection image covers the measuring flume zone fully;
Four, the fish-egg in the measuring flume in the step 3 or seed are transferred in the culture pond;
Five, adopt the Computer Image Processing Survey Software that the view data that step 3 obtains is measured, obtain length data X and the length data Y of 1mm scale in image of fish-egg in the image or seed, be the physical length A that A=X/Y can be calculated fish-egg or seed by formula, unit is mm, has namely finished the live body continuous measurement of cold water fish ovum or seed morphological characters.
Advantage of the present invention: the live body method for continuous measuring of a kind of cold water fish ovum provided by the invention or seed morphological characters, the method of measuring in the Computer Image Processing Survey Software after adopting camera to take pictures, shorten greatly Measuring Time, seed also need not anesthesia simultaneously, avoided drug anesthesia to cause the appearance of deformity individuality and the phenomena of mortality, simultaneously because after photo takes pictures, photo can be amplified tens times to be measured afterwards, greatly avoid the error of manual measurement, reduce the difficulty of fish egg and larva in continuity measurement research process, improved greatly the accuracy of research.
Description of drawings
Fig. 1 is the live body continuous measurement figure of the cold water fish ovum morphological characters in the test one.
Fig. 2 is the live body continuous measurement figure of the cold water seed morphological characters in the test two.
Embodiment
Embodiment one: present embodiment provides the live body method for continuous measuring of a kind of cold water fish ovum or seed morphological characters, specifically finishes according to the following steps:
One, prepares measuring flume, injected water in the measuring flume; Wherein, minimum scale is arranged is the scale of 1mm in the bottom of described measuring flume;
Two, fish-egg or seed are transferred to from culture pond in the measuring flume of the water filling that step 1 obtains;
Three, adopt image capture device perpendicular to the water surface in the measuring flume of step 2 the fish-egg in the measuring flume or seed to be carried out acquisition of image data; Wherein, the zone of collection image covers the measuring flume zone fully;
Four, the fish-egg in the measuring flume in the step 3 or seed are transferred in the culture pond;
Five, adopt the Computer Image Processing Survey Software that the view data that step 3 obtains is measured, obtain length data X and the length data Y of 1mm scale in image of fish-egg in the image or seed, be the physical length A that A=X/Y can be calculated fish-egg or seed by formula, unit is mm, has namely finished the live body continuous measurement of cold water fish ovum or seed morphological characters.
It is fast that the speed of the transfer in the described step 2 of present embodiment is wanted, and prevents that fish-egg or fry open-assembly time in air is long, easily causes the unnecessary death such as anoxic
Camera is for fear of the reflection of the water surface to light perpendicular to the water surface in the measuring flume of step 2 in the described step 3 of present embodiment, and then increases the definition of photo
The zone that gathers image in the described step 3 of present embodiment covers the measuring flume zone fully, is for fear of because indivedual fries go to the trough edge, and the phenomenon that did not photograph.
A kind of cold water fish ovum that present embodiment provides or the live body method for continuous measuring of seed morphological characters have the following advantages: the method for measuring in the Computer Image Processing Survey Software after adopting camera to take pictures, shorten greatly Measuring Time, seed also need not anesthesia simultaneously, avoided drug anesthesia to cause the appearance of deformity individuality and the phenomena of mortality, simultaneously because after photo takes pictures, photo can be amplified tens times to be measured afterwards, greatly avoid the error of manual measurement, reduced fish egg and larva and measured difficulty in the research process in continuity, greatly height the accuracy of research.
Embodiment two: the difference of present embodiment and embodiment one is that the measuring flume of described step 1 is the upper opening of 10cm * 10cm * 3cm, the glass guide channel of the wide 1mm of wall.Other is identical with embodiment one.
The described glass guide channel of present embodiment had both guaranteed to be not easy fragmentation and had also guaranteed light transmission.
Embodiment three: present embodiment and embodiment one or twos' difference is that the height of the water in the described step 1 is 3mm ~ 15mm.Other is identical with embodiment one or two.
The height of the described water of present embodiment is 3mm ~ 15mm, is because the excessively simple and easy anoxic that causes of water, and water is crossed the definition that dark impact is taken pictures, and the height of the control water surface can make the side of fingerling be tiled in the bottom of measuring flume, the error that reduces to measure.
Embodiment four: the difference of one of present embodiment and embodiment one to three is that the bottom surface background of the measuring flume of described step 1 is blue.Other is identical with embodiment one to three.
The bottom surface background of the described measuring flume of present embodiment is blue, is to have large contrast and definition for photographic images.
Embodiment five: the difference of one of present embodiment and embodiment one to four is that the method for the transfer of described step 2 is: adopt landing-spoon that fish-egg or fry are pulled out from raise the pond; The mesh of wherein said landing-spoon is no more than 3mm.Other is identical with embodiment one to four.
The mesh of the described landing-spoon of present embodiment is no more than 3mm, is because the size of fish-egg and seed greater than 3mm, is conveniently fished for.
Embodiment six: the difference of one of present embodiment and embodiment one to five is, in the described step 2 fish-egg or seed are transferred to the measuring flume of the water filling that step 1 obtains from culture pond the time, fish-egg density is 0.5cm
2/ grain ~ 1.5cm
2/ grain; Seed density is 10cm
2/ tail ~ 30cm
2/ tail.Other is identical with embodiment one to five.
The density of the described control fish-egg of present embodiment or seed, can prevent effectively that seed from overlapping each other affects shooting effect.
Embodiment seven: the difference of one of present embodiment and embodiment one to six is that the image capture device of described step 3 is camera or camera.Other is identical with embodiment one to six.
Embodiment eight: the difference of one of present embodiment and embodiment one to seven is, the method for the transfer in the described step 4 is for directly pouring the fish-egg in the measuring flume or seed and water in the culture pond into.Other is identical with embodiment one to seven.
The transfer method of present embodiment is to fish in order to reduce once, has also reduced the damage of operation to fish-egg and fry simultaneously.
Adopt following verification experimental verification effect of the present invention:
The live body method for continuous measuring of a kind of cold water fish ovum or seed morphological characters, specifically finish according to the following steps:
One, prepare the upper opening of 10cm * 10cm * 3cm, the glass guide channel of the wide 1mm of wall injects the high water of 5mm as measuring flume in measuring flume; Wherein, minimum scale is arranged at the bottom of described measuring flume is the scale of 1mm, and the bottom surface background of measuring flume is blue;
Two, the landing-spoon that adopts mesh to be no more than 3mm is pulled fish-egg to be measured out from raise the pond, and is transferred in the measuring flume of the water filling that step 1 obtains;
Three, adopt camera perpendicular to the water surface in the measuring flume of step 2 the fish-egg in the measuring flume to be taken pictures; The zone of wherein, taking pictures covers the measuring flume zone fully;
Four, the fish-egg in the measuring flume in the step 3 is transferred in the culture pond;
Five, the photo that step 3 is obtained imports in the calculator, adopt image measurement software Motic Images Plus to measure, obtain the length data Y of 1mm scale in image in the morphological data X of fish-egg in the image and the measuring flume, be the physical length A that A=X/Y can be calculated fish-egg by formula, unit is mm, has namely finished the live body continuous measurement of cold water fish ovum morphological characters.
By the measurement to 1mm scale, fish-egg 2, fish-egg 3, fish-egg 4, fish-egg 5 and fish-egg 6, obtain the length in the image, resulting length data directly manifests in image, the length L 1 that is respectively the 1mm scale is 31.0 μ m, the length L 2 of fish-egg 2 is 168.6 μ m, and the length L 3 of fish-egg 3 is 166.3 μ m, and the length L 4 of fish-egg 4 is 168.9 μ m, the length L 2 of fish-egg 5 is 188.0 μ m, and the length L 6 of fish-egg 6 is 179.1 μ m; Pass through A=X/Y, wherein, X is the length of fish-egg, Y is the length of scale, the physical length that can calculate fish-egg is respectively: the physical length of fish-egg 2 is 5.438mm, and the physical length of fish-egg 3 is 5.364mm, and the physical length of fish-egg 4 is 5.448mm, the physical length of fish-egg 5 is 6.064mm, and the physical length of fish-egg 6 is 5.777mm.
The live body method for continuous measuring of a kind of cold water fish ovum or seed morphological characters, specifically finish according to the following steps:
One, prepare the upper opening of 10cm * 10cm * 3cm, the glass guide channel of the wide 1mm of wall injects the high water of 10mm as measuring flume in measuring flume; Wherein, minimum scale is arranged at the bottom of described measuring flume is the scale of 1mm, and the bottom surface background of measuring flume is blue;
Two, the landing-spoon that adopts mesh to be no more than 3mm is pulled seed to be measured out from raise the pond, and is transferred in the measuring flume of the water filling that step 1 obtains; Wherein, the side of seed is to be tiled in the measuring flume bottom surface;
Three, adopt camera perpendicular to the water surface in the measuring flume of step 2 the seed in the measuring flume to be taken pictures; The zone of wherein, taking pictures covers the measuring flume zone fully;
Four, the seed in the measuring flume in the step 3 is transferred in the culture pond;
Five, the photo that step 3 is obtained imports in the calculator, adopt image measurement software Motic Images Plus to measure, obtain the length data Y of 1mm scale in image in the morphological data X of seed in the image and the measuring flume, resulting length data directly manifests in image, be the physical length A that A=X/Y can be calculated seed by formula, unit is mm, has namely finished the live body continuous measurement of cold water seed morphological characters.
By 1mm scale, seed 2 are measured, obtain the length in the image, the length L 1 that is respectively the 1mm scale is 37.0 μ m, the length L 2 of seed 2 is 1356.2 μ m; By A=X/Y, wherein, X is the length of seed, and Y is the length of scale, and the physical length that can calculate seed 2 is 36.654mm.
Claims (8)
1. the live body method for continuous measuring of a cold water fish ovum or seed morphological characters, it is characterized in that: the method is specifically finished according to the following steps:
One, prepares measuring flume, injected water in the measuring flume; Wherein, minimum scale is arranged is the scale of 1mm in the bottom of described measuring flume;
Two, fish-egg or seed are transferred to from culture pond in the measuring flume of the water filling that step 1 obtains;
Three, adopt image capture device perpendicular to the water surface in the measuring flume of step 2 the fish-egg in the measuring flume or seed to be carried out acquisition of image data; Wherein, the zone of collection image covers the measuring flume zone fully;
Four, the fish-egg in the measuring flume in the step 3 or seed are transferred in the culture pond;
Five, adopt the Computer Image Processing Survey Software that the view data that step 3 obtains is measured, obtain length data X and the length data Y of 1mm scale in image of fish-egg in the image or seed, be the physical length A that A=X/Y can be calculated fish-egg or seed by formula, unit is mm, has namely finished the live body continuous measurement of cold water fish ovum or seed morphological characters.
2. the live body method for continuous measuring of a kind of cold water fish ovum according to claim 1 or seed morphological characters, it is characterized in that: the measuring flume of described step 1 is the upper opening of 10cm * 10cm * 3cm, the glass guide channel of the wide 1mm of wall.
3. the live body method for continuous measuring of a kind of cold water fish ovum according to claim 1 and 2 or seed morphological characters, it is characterized in that: the height of the water in the described step 1 is 3mm ~ 15mm.
4. the live body method for continuous measuring of a kind of cold water fish ovum according to claim 1 and 2 or seed morphological characters is characterized in that: the bottom surface background of the measuring flume of described step 1 is for blue.
5. the live body method for continuous measuring of a kind of cold water fish ovum according to claim 1 or seed morphological characters, it is characterized in that: the method for the transfer of described step 2 is: adopt landing-spoon that fish-egg or fry are pulled out from raise the pond; The mesh of wherein said landing-spoon is no more than 3mm.
6. according to claim 1, the live body method for continuous measuring of 2 or 5 described a kind of cold water fish ovum or seed morphological characters, it is characterized in that: after the transfer in the described step 2 in the measuring flume fish-egg density be 0.5cm
2/ grain ~ 1.5cm
2/ grain, seed density is 10cm
2/ tail ~ 30cm
2/ tail.
7. the live body method for continuous measuring of a kind of cold water fish ovum according to claim 1 and 2 or seed morphological characters, it is characterized in that: the image capture device of described step 3 is camera or camera.
8. the live body method for continuous measuring of a kind of cold water fish ovum according to claim 1 and 2 or seed morphological characters is characterized in that: the method for the transfer in the described step 4 is for directly pouring the fish-egg in the measuring flume or seed and water in the culture pond into.
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Cited By (2)
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| CN103583440A (en) * | 2013-11-21 | 2014-02-19 | 中国水产科学研究院黑龙江水产研究所 | Method for fast and physically distinguishing quality of mature ova of hucho taimen |
| CN104823891A (en) * | 2015-05-25 | 2015-08-12 | 江苏农牧科技职业学院 | Recognition method and device for automatically distinguishing male and female spiny eels |
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Cited By (3)
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| CN104823891A (en) * | 2015-05-25 | 2015-08-12 | 江苏农牧科技职业学院 | Recognition method and device for automatically distinguishing male and female spiny eels |
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Application publication date: 20130417 |