CN102515888A - Preparation method of microbial organic fertilizer - Google Patents
Preparation method of microbial organic fertilizer Download PDFInfo
- Publication number
- CN102515888A CN102515888A CN2011104186167A CN201110418616A CN102515888A CN 102515888 A CN102515888 A CN 102515888A CN 2011104186167 A CN2011104186167 A CN 2011104186167A CN 201110418616 A CN201110418616 A CN 201110418616A CN 102515888 A CN102515888 A CN 102515888A
- Authority
- CN
- China
- Prior art keywords
- fertilizer
- soil
- phanerochaete chrysosporium
- microbial organic
- organic fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of microbial organic fertilizer, which comprises the following steps: inoculating Phanerochaete chrysosporium solution in rotten rapeseed cake fertilizer, and carrying out solid culture; mixing bentonite, Bacillus thuringlensis wettable powder and the obtained rapeseed cake fertilizer growing Phanerochaete chrysosporium, and air-drying to obtain the microbial organic fertilizer. The preparation method of the invention is simple and convenient, the prepared fertilizer has a soil improvement effect, and can regulate soil nutrient supply through regulating soil microenvironment, and improve fertilizer utilization rate and prolong fertilizer effect.
Description
Technical field
The present invention relates to fertilizer field, relate in particular to a kind of preparation method of microbial organic fertilizer.
Background technology
Soil is the vital integral part of human foods chain, is the basis of agricultural production system, and it is not only the important medium of plant growth and is keeping crop-producing power, affects quality and the plant even the human beings'health of environment.Yet a large amount of use chemical fertilizer of people and agricultural chemicals to be pursuing higher output for a long time, and ignore the protection and the adjusting of ecological environment of soil; Cause the soil ecology structure to be destroyed, the serious farming property of land deterioration descends the nutrient deficiency in the soil or unbalanced year by year; Useful microbe reduces; Soil water-reataining fertilizer-keeping ability variation or the like, the soil ecology problem that these are a series of has caused very big threat to the raising of crop products quality.Therefore press for the usage quantity that reduces chemical fertilizer and chemical pesticide, the objectionable impurities in the fixing soil, nutritive elements such as releases phosphorus potassium; Improve the soil from root; Improve quality and degeneration-resistant and ability disease and insect resistance of crop, improve food safety, the protection environment.
Microbial fertilizer is a kind of new-type fertilizer that develops rapidly in recent years, is that the vital movement with mikrobe causes farm crop to obtain the biological products of specific fertilizer effect, has the not available advantages of other fertilizer such as nontoxic, harmless, pollution-free, that fertilizer efficiency is long.Through in fertilizer, adding mikrobe, when introducing new species, promote the growth of indigenous microorganism, utilize the vital movement of mikrobe, improve the physico-chemical property of soil, regulate the nutrient supply ability of soil, promote the benign cycle of ecological environment of soil.The present microbial fertilizer that is applied to agriculture aspect mainly concentrates on single or composite microbe fertilizers such as fixed nitrogen, phosphorus decomposing, potassium decomposing; To different soil characteristics, more useful microbe is applied to be undoubtedly in the microbial fertilizer problem that is worth research.
Summary of the invention
Technical problem to be solved by this invention is: to the deficiency of prior art, a kind of preparation method of microbial organic fertilizer is provided, the fertilizer that this method is produced has soil improvement action, and can regulate the soil nutrient supply through regulating the soil micro-ecosystem environment.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A kind of preparation method of microbial organic fertilizer may further comprise the steps:
(1) the Phanerochaete chrysosporium liquid after will cultivating is seeded in the rapeseed cake fertilizer that becomes thoroughly decomposed; The consumption of said Phanerochaete chrysosporium liquid is 2～3 mass parts; The consumption of said rapeseed cake fertilizer is 10～15 mass parts; Water cut with the two mixture is controlled at 40%～50% again, in aerobic, temperature is under 35 ℃～39 ℃ the condition mixture to be cultivated, and incubation time is 2～7 days;
(2) growth that wilkinite, bacillus thuringiensis wettable powder and above-mentioned steps (1) is obtained has the rapeseed cake fertilizer of Phanerochaete chrysosporium to mix; Add the wilkinite of 0.5～0.75 mass parts and the bacillus thuringiensis wettable powder of 0.005～0.015 mass parts in the rapeseed cake fertilizer of every mass parts respectively, obtain microbial organic fertilizer after air-dry.
Among the above-mentioned preparation method, the mass ratio of nitrogen, phosphorus, potassium is preferably (3～4) in the said rapeseed cake fertilizer: (1～2): (1～2).
Among the above-mentioned preparation method, preferably, in the said step (2), the air-dry microbial organic fertilizer that is meant that control makes at last is moisture powdery, and water cut is controlled at below 30%.
As the further improvement to technique scheme, said Phanerochaete chrysosporium liquid preferably obtains through following method:
(1) the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium carried out activation;
(2) the Phanerochaete chrysosporium spore after the activation is dissolved in the sterilized water, processes the Phanerochaete chrysosporium suspension, it is 10 that said Phanerochaete chrysosporium suspension contains spore count
4Individual/mL～10
6Individual/mL;
(3) with the above-mentioned Phanerochaete chrysosporium suspension inoculation that makes to the inorganic nutrient solution of Kirk; Every 100mL nutrient solution inoculation 2mL～4mL Phanerochaete chrysosporium suspension; With mixed solution shaking culture 60h～72h under 35 ℃～39 ℃, 120r/min～150r/min condition, obtain Phanerochaete chrysosporium liquid then.
Prepared according to the method described above microbial fertilizer is a powdery, and water ratio is below 30%, and total living bacteria count is no less than 0.2 hundred million/g, and Phanerochaete chrysosporium and bacillus thuringiensis number average are no less than 0.01 hundred million/g.
Compared with prior art; Advantage of the present invention is: the present invention has added wilkinite as water-reataining fertilizer-keeping agent in rapeseed cake fertilizer, can improve the structure and the character of soil agreegate effectively, improves the ventilation property of soil; Strengthen the preserve moisture and fertility ability of soil, reduce nutrient loss to a certain extent; The Phanerochaete chrysosporium of adding in the fertilizer can be regulated the soil micro-ecosystem environment, and the slowly-releasing of control fertilizer is regulated the soil nutrient supply; In addition, Phanerochaete chrysosporium has stronger adsorption to objectionable impuritiess such as some heavy metals, organism, and stronger using value is also arranged in the contaminated soil reparation; The bacillus thuringiensis of adding in the fertilizer as a kind of biotic pesticide, is used on agricultural; But use as foliar insecticide mostly, persistence is relatively poor, and the present invention is inoculated into it and introduces soil in fertilizer; Utilize bacillus thuringiensis to kill harmful microorganism in the soil; Thereby reduce the generation of crop pest, because bacillus thuringiensis breeds willful murder worm persistent in soil.Generally speaking, preparation method of the present invention is simple, enforcement is convenient, and the product prepn cost is low, and effect is good, and is environment friendly and pollution-free.
Embodiment
Below in conjunction with specific embodiment the present invention is further described.
Embodiment 1:
A kind of preparation method of microbial organic fertilizer of the present invention may further comprise the steps:
1, Phanerochaete chrysosporium bacterium liquid is cultivated in preparation
(1) actication of culture: the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium carried out activation;
(2) the Phanerochaete chrysosporium spore after the activation is dissolved in the sterilized water, it is subsequent use to process bacteria suspension, and its contained spore count is 10
4Individual/mL～10
6Individual/mL;
(3) with made Phanerochaete chrysosporium suspension inoculation to the inorganic nutrient solution of Kirk, every 100mL nutrient solution inoculation 3mL Phanerochaete chrysosporium suspension, then with mixed solution at 39 ℃, shaking culture 72h under the 120r/min condition;
2, take by weighing the rapeseed cake fertilizer that becomes thoroughly decomposed (mass ratio of nitrogen, phosphorus, potassium is 3.5: 1: 1) 20Kg, add the Phanerochaete chrysosporium bacterium liquid after 4000mL cultivates, controlling moisture content is 40%～50%; Stir; Cultivate 3d down at 39 ℃, in culturing process, regularly stir, guarantee aerobic conditions;
3, after cultivation finishes, getting the rapeseed cake fertilizer that growth has Phanerochaete chrysosporium, every 3.0Kg adds 1.5Kg wilkinite, 25g bacillus thuringiensis (8000IU/mg) wettable powder; Mix; Air-dry, water cut is controlled at below 30%, obtain microbial organic fertilizer.
The component of the microbial organic fertilizer that above-mentioned present embodiment makes and mass percent thereof are:
Embodiment 2:
A kind of preparation method of microbial organic fertilizer of the present invention may further comprise the steps:
1, Phanerochaete chrysosporium bacterium liquid is cultivated in preparation
(1) actication of culture: the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium carried out activation;
(2) the Phanerochaete chrysosporium spore after the activation is dissolved in the sterilized water, it is subsequent use to process bacteria suspension, and its contained spore count is 10
4Individual/mL～10
6Individual/mL;
(3) with made Phanerochaete chrysosporium suspension inoculation to the inorganic nutrient solution of Kirk, every 100mL nutrient solution inoculation 4mL Phanerochaete chrysosporium suspension, then with mixed solution at 36 ℃, shaking culture 70h under the 150r/min condition;
2, take by weighing the rapeseed cake fertilizer that becomes thoroughly decomposed (mass ratio of nitrogen, phosphorus, potassium is 3.5: 1: 1) 20Kg, add the Phanerochaete chrysosporium bacterium liquid after 4000mL cultivates, controlling moisture content is 40%～50%; Stir; Cultivate 5d down at 36 ℃, in culturing process, regularly stir, guarantee aerobic conditions;
3, after cultivation finishes, getting the rapeseed cake fertilizer that growth has Phanerochaete chrysosporium, every 3.0Kg adds 1.5Kg wilkinite, 25g bacillus thuringiensis (8000IU/mg) wettable powder and 200g water; Mix; Air-dry, water cut is controlled at below 30%, obtain microbial organic fertilizer.
The component of the microbial organic fertilizer that above-mentioned present embodiment makes and quality percentage composition thereof are:
Embodiment 3:
Microbial organic fertilizer (the T that embodiment 1 is made
1), the microbial organic fertilizer (T that makes of embodiment 2
2) fertile as base manure or cave, be applied to vega production, cover immediately after applying, every mu of consumption is 20Kg, microbial organic fertilizer does not contact chemical fertilizer, does not mix use with chemical fertilizer; Under all identical condition of other condition of maintenance, be control samples (CK) only to use the rapeseed cake fertilizer that becomes thoroughly decomposed, in handling vega, compare experiment.Gather the pedotheque of tobacco different growing stages respectively; The group's of comprising phase (transplanting 30d), prosperous long-term (50d), (57d), bottom leaf ripening stage (69d), middle leaf ripening stage (93d), upper leaf ripening stage (110d) when pinching; Measure soil enzyme activities and (gather the tobacco bright soil in each vegetative period; Utilize 3; 5-dinitrosalicylic acid colorimetric method for determining soil saccharase is active, sucrase active with 24h after the milligram numerical table of 1g soil glucose show), quick-acting potassium content (gathering the tobacco rhizosphere soil in each vegetative period) and nitrogen content with ammonium acetate lixiviate atomic absorption spectroscopy determination soil quick-acting potassium content; Gather sophisticated down, in, upper tobacco leaf, measure the full potassium content of tobacco leaf; The research microbial fertilizer is to the influence of ecological environment of soil and cigarette quality.
Measure the result and see table 1～table 5, than control samples, the crop of using microbial organic fertilizer of the present invention is at period of drought, and topsoil soils obviously contrasts moistening, soft; Than control samples, use microbial organic fertilizer of the present invention after, soil saccharase improves (seeing the following form 1) in that the whole growing stage of tobacco is active, ripe tobacco leaf potassium percentage composition improves about 10% (seeing table 2); In the phase of pinching to the middle leaf ripening stage of tobacco growing, the soil quick-acting potassium content of embodiment 1 and embodiment 2 increases (seeing table 3) than control samples; Microbial fertilizer to the regulation and control of soil nitrogen and relevant saccharase mainly concentrate on prosperous for a long time to the prematuration period, and get into the ripe later stage in group's phase and tobacco, microbial fertilizer is to the influence of soil nitrogen and relevant saccharase not significantly (seeing table 4 and table 5).It is thus clear that microbial organic fertilizer of the present invention has regulating and controlling effect to transformation of soil nitrogen enzymic activity and soil nitrate nitrogen content in the certain period.In addition, tobacco physicochemical character and quality characteristic after microbial organic fertilizer of the present invention is used obviously improve, and the disease and pest phenomenon obviously alleviates.
Table 1 microbial organic fertilizer is to the active influence of each soil saccharase in vegetative period of tobacco
Table 2 microbial organic fertilizer is to the influence of ripe tobacco leaf potassium percentage composition
| Handle | Upper leaf (%) | Middle leaf (%) | Bottom leaf (%) |
| CK | 1.53 | 2.37 | 3.18 |
| T 1 | 2.04 | 2.72 | 4.10 |
| T 2 | 1.62 | 2.51 | 3.60 |
Table 3 microbial organic fertilizer is to the influence of soil quick-acting potassium content
Table 4 microbial fertilizer is to the influence of nitrate reductase activity in soil
Table 5 microbial fertilizer is to the influence of soil nitrate nitrogen content
Claims (1)
1. the preparation method of a microbial organic fertilizer is characterized in that, may further comprise the steps:
(1) the Phanerochaete chrysosporium liquid after will cultivating is seeded in the rapeseed cake fertilizer that becomes thoroughly decomposed; The consumption of said Phanerochaete chrysosporium liquid is 2～3 mass parts; The consumption of said rapeseed cake fertilizer is 10～15 mass parts; Water cut with the two mixture is controlled at 40%～50% again, in aerobic, temperature is under 35 ℃～39 ℃ the condition mixture to be cultivated, and incubation time is 2～7 days;
(2) growth that wilkinite, bacillus thuringiensis wettable powder and above-mentioned steps (1) is obtained has the rapeseed cake fertilizer of Phanerochaete chrysosporium to mix; Add the wilkinite of 0.5～0.75 mass parts and the bacillus thuringiensis wettable powder of 0.005～0.015 mass parts in the rapeseed cake fertilizer of every mass parts respectively, obtain microbial organic fertilizer after air-dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110418616 CN102515888B (en) | 2011-12-14 | 2011-12-14 | Preparation method of microbial organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110418616 CN102515888B (en) | 2011-12-14 | 2011-12-14 | Preparation method of microbial organic fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102515888A true CN102515888A (en) | 2012-06-27 |
| CN102515888B CN102515888B (en) | 2013-06-05 |
Family
ID=46287032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110418616 Expired - Fee Related CN102515888B (en) | 2011-12-14 | 2011-12-14 | Preparation method of microbial organic fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102515888B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087527A (en) * | 2014-06-20 | 2014-10-08 | 沈阳壅源生物有机肥有限公司 | Bacillus thuringiensis, preparation method for solid medium of Bacillus thuringiensis, and application of Bacillus thuringiensis |
| CN109534906A (en) * | 2018-12-30 | 2019-03-29 | 重庆油橄榄研究院有限公司 | Olive waste material organic fertilizer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001019940A1 (en) * | 1999-09-16 | 2001-03-22 | Dirk Van Barneveld | Soil improver composition and plant growth enhancer |
| RU2192403C2 (en) * | 2000-06-23 | 2002-11-10 | Иркутский институт химии СО РАН | Method of organomineral fertilizer preparing |
| CN102079675A (en) * | 2010-12-03 | 2011-06-01 | 张新雄 | Magnetic biofertilizer and preparation method |
| CN102093126A (en) * | 2010-12-03 | 2011-06-15 | 张新雄 | Method for producing bio-fertilizer from waste organic matters |
-
2011
- 2011-12-14 CN CN 201110418616 patent/CN102515888B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001019940A1 (en) * | 1999-09-16 | 2001-03-22 | Dirk Van Barneveld | Soil improver composition and plant growth enhancer |
| RU2192403C2 (en) * | 2000-06-23 | 2002-11-10 | Иркутский институт химии СО РАН | Method of organomineral fertilizer preparing |
| CN102079675A (en) * | 2010-12-03 | 2011-06-01 | 张新雄 | Magnetic biofertilizer and preparation method |
| CN102093126A (en) * | 2010-12-03 | 2011-06-15 | 张新雄 | Method for producing bio-fertilizer from waste organic matters |
Non-Patent Citations (2)
| Title |
|---|
| SUNITA GAIND 等: "Quality evaluation of co-composted wheat straw,poultry droppings and oil seed cakes", 《BIODEGRADATION》 * |
| 黄丹莲 等: "白腐菌应用于堆肥处理含木质素废物的研究", 《环境污染治理技术与设备》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087527A (en) * | 2014-06-20 | 2014-10-08 | 沈阳壅源生物有机肥有限公司 | Bacillus thuringiensis, preparation method for solid medium of Bacillus thuringiensis, and application of Bacillus thuringiensis |
| CN109534906A (en) * | 2018-12-30 | 2019-03-29 | 重庆油橄榄研究院有限公司 | Olive waste material organic fertilizer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102515888B (en) | 2013-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103848698B (en) | A kind of biological organic fertilizer utilizing natural pond slag aerobic fermentation to prepare and preparation method thereof | |
| CN102229900B (en) | Compound probiotics preparation for biological organic fertilizers and preparation method thereof | |
| CN100396652C (en) | Biological orgamic fertilizer and prepn thereof | |
| CN103740693B (en) | A kind of straw decomposing inoculant and preparation method thereof | |
| CN104447040B (en) | A kind of three sour mixed fermentation biological organic fertilizers and preparation method thereof and the application in tobacco planting | |
| CN107282628A (en) | A kind of composite modifying agent for repairing farmland pollution soil and preparation method thereof | |
| CN101875571B (en) | Method for preparing enhanced liquid microbial organic fertilizer | |
| CN104059671A (en) | Facility secondary NO3<-> salinized soil improver, preparation method thereof and improving method | |
| CN104609997A (en) | Manufacturing process and application method of ginseng bio-organic fertilizer | |
| CN104560045B (en) | Microbial bacterial agent and chemical fertilizer excessively use growth-promoting secondary saline and alkaline and prone soil improved application | |
| CN106083453A (en) | A kind of biomass organic fertilizer and preparation method thereof | |
| CN105347926A (en) | Preparation method for microbial organic soil bactericidal and insecticidal warming conditioning agent | |
| CN109762765B (en) | Decomposed solid fermentation microbial inoculum and application thereof in agricultural wastes | |
| CN105481489B (en) | A kind of Bacillus subtillis bio-bacterial manure and preparation method thereof | |
| CN109180316A (en) | A kind of rice Special compound microbial fertilizer and preparation method thereof | |
| CN105503391B (en) | A kind of prevention and treatment tomato powdery mildew of cucumber function foliar fertilizer and application | |
| CN110305671A (en) | One kind is for facilities vegetable continuous cropping soil biological modification agent and its preparation and application method | |
| CN104496573B (en) | A kind of grape special biological and ecological methods to prevent plant disease, pests, and erosion composite microbiological fertilizer, production method and application | |
| CN105670963A (en) | Compound microbial fertilizer and preparation method thereof | |
| CN102827774B (en) | Microbial inoculum and application thereof in improvement of facility agriculture | |
| CN102432355A (en) | Banana stem organic fertilizer and preparation method thereof | |
| CN105859338A (en) | Functional microbial soil conditioner and preparation method thereof | |
| CN102503712B (en) | Microbial organic fertilizer and application thereof | |
| CN101817709A (en) | Biological organic fertilizer with disease preventing function and preparation method thereof | |
| CN108456021A (en) | A kind of biological organic fertilizer and preparation method thereof with decomposed stalk effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130605 Termination date: 20131214 |