CN102515888B - Preparation method of microbial organic fertilizer - Google Patents
Preparation method of microbial organic fertilizer Download PDFInfo
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- CN102515888B CN102515888B CN 201110418616 CN201110418616A CN102515888B CN 102515888 B CN102515888 B CN 102515888B CN 201110418616 CN201110418616 CN 201110418616 CN 201110418616 A CN201110418616 A CN 201110418616A CN 102515888 B CN102515888 B CN 102515888B
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- phanerochaete chrysosporium
- fertilizer
- soil
- rapeseed cake
- microbial organic
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Abstract
The invention discloses a preparation method of microbial organic fertilizer, which comprises the following steps: inoculating Phanerochaete chrysosporium solution in rotten rapeseed cake fertilizer, and carrying out solid culture; mixing bentonite, Bacillus thuringlensis wettable powder and the obtained rapeseed cake fertilizer growing Phanerochaete chrysosporium, and air-drying to obtain the microbial organic fertilizer. The preparation method of the invention is simple and convenient, the prepared fertilizer has a soil improvement effect, and can regulate soil nutrient supply through regulating soil microenvironment, and improve fertilizer utilization rate and prolong fertilizer effect.
Description
Technical field
The present invention relates to fertilizer field, relate in particular to a kind of preparation method of microbial organic fertilizer.
Background technology
Soil is the vital integral part of human foods chain, is the basis of agricultural production system, and it is not only the important medium of plant growth and is keeping crop-producing power, affects the even mankind's health of the quality of environment and plant.Yet; the a large amount of use chemical fertilizer of people and agricultural chemicals are to pursue higher output for a long time; and ignore protection and the adjusting of ecological environment of soil; cause the soil ecology structure to be destroyed, the serious farming of land deterioration descends year by year, the nutrient deficiency in soil or unbalanced; beneficial microorganism reduces; soil water-reataining fertilizer-keeping ability variation etc., this a series of soil ecological problem has caused very large threat to the raising of crop product quantity.Therefore in the urgent need to reducing the usage quantity of chemical fertilizer and chemical pesticide, the objectionable impurities in fixing soil discharges the nutritive elements such as phosphorus potassium; improve soil from root; improve quality and degeneration-resistant and ability disease and insect resistance of crop, improve food safety, protection of the environment.
Microbial fertilizer is a kind of new-type fertilizer that develops rapidly in recent years, is that the vital movement with microorganism causes farm crop to obtain the biological products of specific fertilizer effect, has the not available advantages of other fertilizer such as nontoxic, harmless, pollution-free, that fertilizer efficiency is long.By add microorganism in fertilizer, when introducing new species, promote the growth of indigenous microorganism, utilize the vital movement of microorganism, improve the physico-chemical property of soil, regulate the nutrient supply ability of soil, promote the benign cycle of ecological environment of soil.The present microbial fertilizer that is applied to agriculture aspect mainly concentrates on the single or composite microbe fertilizers such as fixed nitrogen, phosphorus decomposing, potassium decomposing, for different soil characteristics, more beneficial microorganism is applied to be undoubtedly in microbial fertilizer a problem that is worth research.
Summary of the invention
Technical problem to be solved by this invention is: for the deficiencies in the prior art, provide a kind of preparation method of microbial organic fertilizer, the fertilizer that the method is produced has soil improvement action, and can regulate the soil nutrient supply by regulating soil microenvironment.
For solving the problems of the technologies described above, the present invention by the following technical solutions:
A kind of preparation method of microbial organic fertilizer comprises the following steps:
(1) the Phanerochaete chrysosporium liquid after cultivating is seeded in the rapeseed cake fertilizer that becomes thoroughly decomposed, the consumption of described Phanerochaete chrysosporium liquid is 2～3 mass parts, the consumption of described rapeseed cake fertilizer is 10～15 mass parts, both the water content of mixture is controlled at 40%～50% again, be under the condition of 35 ℃～39 ℃, mixture to be cultivated in aerobic, temperature, incubation time is 2～7 days;
(2) growth that wilkinite, Bacillus thuringiensis wettable powder and above-mentioned steps (1) is obtained has the rapeseed cake fertilizer of Phanerochaete chrysosporium to mix, add respectively the wilkinite of 0.5～0.75 mass parts and the Bacillus thuringiensis wettable powder of 0.005～0.015 mass parts in the rapeseed cake fertilizer of every mass parts, obtain microbial organic fertilizer after air-dry.
In above-mentioned preparation method, in described rapeseed cake fertilizer, the mass ratio of nitrogen, phosphorus, potassium is preferably (3～4): (1～2): (1～2).
In above-mentioned preparation method, preferably, in described step (2), air-dryly refer to control the microbial organic fertilizer that makes at last and be moisture powdery, and water content is controlled at below 30%.
As to further improvement in the technical proposal, described Phanerochaete chrysosporium liquid preferably obtains by the following method:
(1) the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium activated;
(2) the Phanerochaete chrysosporium spore after activating is dissolved in sterilized water, makes the Phanerochaete chrysosporium suspension, and it is 10 that described Phanerochaete chrysosporium suspension contains spore count
4Individual/mL～10
6Individual/mL;
(3) with the above-mentioned Phanerochaete chrysosporium suspension inoculation that makes to Kirk minimal media liquid, every 100mL nutrient solution inoculation 2mL～4mL Phanerochaete chrysosporium suspension, then with mixed solution shaking culture 60h～72h under 35 ℃～39 ℃, 120r/min～150r/min condition, obtain Phanerochaete chrysosporium liquid.
Prepared microbial fertilizer is powdery according to the method described above, and water ratio is below 30%, and total living bacteria count is no less than 0.2 hundred million/g, and Phanerochaete chrysosporium and bacillus thuringiensis number average are no less than 0.01 hundred million/g.
Compared with prior art, advantage of the present invention is: the present invention has added wilkinite as water-reataining fertilizer-keeping agent in rapeseed cake fertilizer, can effectively improve structure and the character of soil agreegate, improves the ventilation property of soil, strengthen the preserve moisture and fertility ability of soil, reduce to a certain extent nutrient loss; The Phanerochaete chrysosporium of adding in fertilizer can be regulated soil microenvironment, controls the slowly-releasing of fertilizer, regulates the soil nutrient supply; In addition, Phanerochaete chrysosporium has stronger adsorption to objectionable impuritiess such as some heavy metals, organism, and stronger using value is also arranged in the contaminated soil reparation; The bacillus thuringiensis of adding in fertilizer, as a kind of biotic pesticide, use on agricultural, but mostly use as foliar insecticide, persistence is relatively poor, and the present invention is inoculated into it and introduces soil in fertilizer, utilize bacillus thuringiensis to kill harmful microorganism in soil, thereby reduce the generation of crop pest, because bacillus thuringiensis breeds in soil, the effect of willful murder worm is lasting.Generally speaking, preparation method of the present invention is simple, enforcement is convenient, and the product preparation cost is low, and effect is good, and is environment friendly and pollution-free.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1:
A kind of preparation method of microbial organic fertilizer of the present invention comprises the following steps:
1, Phanerochaete chrysosporium bacterium liquid is cultivated in preparation
(1) actication of culture: the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium activated;
(2) the Phanerochaete chrysosporium spore after activating is dissolved in sterilized water, makes bacteria suspension standby, and its contained spore count is 10
4Individual/mL～10
6Individual/mL;
(3) with made Phanerochaete chrysosporium suspension inoculation to Kirk minimal media liquid, every 100mL nutrient solution inoculation 3mL Phanerochaete chrysosporium suspension, then with mixed solution at 39 ℃, shaking culture 72h under the 120r/min condition;
2, take rapeseed cake fertilizer (mass ratio of nitrogen, phosphorus, potassium is 3.5: 1: 1) 20Kg, add the Phanerochaete chrysosporium bacterium liquid after 4000mL cultivates, control water content 40%～50%, stir, cultivate 3d under 39 ℃, regularly stir in culturing process, ensure oxygen condition;
3, after cultivation finishes, getting the rapeseed cake fertilizer that growth has Phanerochaete chrysosporium, every 3.0Kg adds 1.5Kg wilkinite, 25g bacillus thuringiensis (8000IU/mg) wettable powder, mix, air-dry, water content is controlled at below 30%, obtain microbial organic fertilizer.
The component of the microbial organic fertilizer that above-mentioned the present embodiment makes and mass percent thereof are:
Embodiment 2:
A kind of preparation method of microbial organic fertilizer of the present invention comprises the following steps:
1, Phanerochaete chrysosporium bacterium liquid is cultivated in preparation
(1) actication of culture: the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium activated;
(2) the Phanerochaete chrysosporium spore after activating is dissolved in sterilized water, makes bacteria suspension standby, and its contained spore count is 10
4Individual/mL～10
6Individual/mL;
(3) with made Phanerochaete chrysosporium suspension inoculation to Kirk minimal media liquid, every 100mL nutrient solution inoculation 4mL Phanerochaete chrysosporium suspension, then with mixed solution at 36 ℃, shaking culture 70h under the 150r/min condition;
2, take rapeseed cake fertilizer (mass ratio of nitrogen, phosphorus, potassium is 3.5: 1: 1) 20Kg, add the Phanerochaete chrysosporium bacterium liquid after 4000mL cultivates, control water content 40%～50%, stir, cultivate 5d under 36 ℃, regularly stir in culturing process, ensure oxygen condition;
3, after cultivation finishes, getting the rapeseed cake fertilizer that growth has Phanerochaete chrysosporium, every 3.0Kg adds 1.5Kg wilkinite, 25g bacillus thuringiensis (8000IU/mg) wettable powder and 200g water, mix, air-dry, water content is controlled at below 30%, obtain microbial organic fertilizer.
The component of the microbial organic fertilizer that above-mentioned the present embodiment makes and quality percentage composition thereof are:
Embodiment 3:
Microbial organic fertilizer (the T that embodiment 1 is made
1), the microbial organic fertilizer (T that makes of embodiment 2
2) fertile as base manure or cave, be applied to vega production, cover immediately after applying, every mu of consumption is 20Kg, microbial organic fertilizer does not contact chemical fertilizer, does not mix use with chemical fertilizer; Keeping other condition all under identical condition, only to use rapeseed cake fertilizer as control sample (CK), compare experiment in processing vega.gather respectively the pedotheque of tobacco different growing stages, the group's of comprising phase (transplanting 30d), prosperous long-term (50d), when pinching (57d), the bottom leaf ripening stage (69d), the middle leaf ripening stage (93d), the upper leaf ripening stage (110d), measure soil enzyme activities and (gather the tobacco bright soil in each vegetative period, utilize 3, the 5-dinitrosalicylic Acid Colorimetry is measured soil invertase activity, sucrase active with 24h after 1g soil glucose the milligram number represent), quick-acting potassium content (gathers the tobacco rhizosphere soil in each vegetative period, with ammonium acetate lixiviate atomic absorption spectroscopy determination Soil Available potassium content), and nitrogen content, gather ripe under, in, upper tobacco leaf, measure the full potassium content of tobacco leaf, the impact of microorganisms fertilizer on ecological environment of soil and cigarette quality.
Measurement result sees Table 1～table 5, than control sample, uses the crop of microbial organic fertilizer of the present invention at period of drought, and topsoil soils obviously contrasts moistening, soft; Than control sample, after using microbial organic fertilizer of the present invention, soil saccharase is in the tobacco active raising of whole growing stage (seeing the following form 1), and Mature Tobacco Leaves potassium percentage composition improves 10% left and right (seeing Table 2); To the middle leaf ripening stage, the Soil Available potassium content of embodiment 1 and embodiment 2 increases (seeing Table 3) than control sample at the Topping Stage of tobacco growing; Microbial fertilizer to soil nitrogen and relevant transform Enzyme Regulation mainly concentrate on prosperous for a long time to the prematuration period, and enter the ripe later stage in group's phase and tobacco, microbial fertilizer is on the impact of soil nitrogen and relevant saccharase not significantly (see Table 4 and table 5).As seen, microbial organic fertilizer of the present invention has regulating and controlling effect to transformation of soil nitrogen enzymic activity and content of soil nitrate-N in the certain period.In addition, tobacco physicochemical character and quality characteristic after microbial organic fertilizer of the present invention is used obviously improve, and the disease and pest phenomenon obviously alleviates.
The impact of table 1 microbial organic fertilizer on tobacco soil invertase activity in each in vegetative period
The impact of table 2 microbial organic fertilizer on Mature Tobacco Leaves potassium percentage composition
| Process | Upper leaf (%) | Middle leaf (%) | Bottom leaf (%) |
| CK | 1.53 | 2.37 | 3.18 |
| T 1 | 2.04 | 2.72 | 4.10 |
| T 2 | 1.62 | 2.51 | 3.60 |
The impact of table 3 microbial organic fertilizer on the Soil Available potassium content
The impact of table 4 microbial fertilizer on nitrate reductase activity in soil
The impact of table 5 microbial fertilizer on content of soil nitrate-N
Claims (1)
1. the preparation method of a microbial organic fertilizer, is characterized in that, comprises the following steps:
(1) the Phanerochaete chrysosporium liquid after cultivating is seeded in the rapeseed cake fertilizer that becomes thoroughly decomposed, the consumption of described Phanerochaete chrysosporium liquid is 2～3 mass parts, the consumption of described rapeseed cake fertilizer is 10～15 mass parts, both the water content of mixture is controlled at 40%～50% again, be under the condition of 35 ℃～39 ℃, mixture to be cultivated in aerobic, temperature, incubation time is 2～7 days;
(2) growth that wilkinite, Bacillus thuringiensis wettable powder and above-mentioned steps (1) is obtained has the rapeseed cake fertilizer of Phanerochaete chrysosporium to mix, add respectively the wilkinite of 0.5～0.75 mass parts and the Bacillus thuringiensis wettable powder of 0.005～0.015 mass parts in the rapeseed cake fertilizer of every mass parts, obtain microbial organic fertilizer after air-dry;
Described Phanerochaete chrysosporium liquid obtains by the following method:
(1) the female kind in the dull and stereotyped activation medium of access potato of Phanerochaete chrysosporium activated;
(2) the Phanerochaete chrysosporium spore after activating is dissolved in sterilized water, makes the Phanerochaete chrysosporium suspension, and it is 10 that described Phanerochaete chrysosporium suspension contains spore count
4Individual/mL～10
6Individual/mL;
(3) with the above-mentioned Phanerochaete chrysosporium suspension inoculation that makes to Kirk minimal media liquid, every 100mL nutrient solution inoculation 2mL～4mL Phanerochaete chrysosporium suspension, then with mixed solution shaking culture 60h～72h under 35 ℃～39 ℃, 120r/min～150r/min condition, obtain Phanerochaete chrysosporium liquid.
Priority Applications (1)
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|---|---|---|---|
| CN 201110418616 CN102515888B (en) | 2011-12-14 | 2011-12-14 | Preparation method of microbial organic fertilizer |
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|---|---|---|---|
| CN 201110418616 CN102515888B (en) | 2011-12-14 | 2011-12-14 | Preparation method of microbial organic fertilizer |
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| CN102515888A CN102515888A (en) | 2012-06-27 |
| CN102515888B true CN102515888B (en) | 2013-06-05 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104087527B (en) * | 2014-06-20 | 2017-01-11 | 沈阳壅源生物有机肥有限公司 | Bacillus thuringiensis, and preparation method for solid medium of Bacillus thuringiensis |
| CN109534906A (en) * | 2018-12-30 | 2019-03-29 | 重庆油橄榄研究院有限公司 | Olive waste material organic fertilizer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001019940A1 (en) * | 1999-09-16 | 2001-03-22 | Dirk Van Barneveld | Soil improver composition and plant growth enhancer |
| RU2192403C2 (en) * | 2000-06-23 | 2002-11-10 | Иркутский институт химии СО РАН | Method of organomineral fertilizer preparing |
| CN102079675A (en) * | 2010-12-03 | 2011-06-01 | 张新雄 | Magnetic biofertilizer and preparation method |
| CN102093126A (en) * | 2010-12-03 | 2011-06-15 | 张新雄 | Method for producing bio-fertilizer from waste organic matters |
-
2011
- 2011-12-14 CN CN 201110418616 patent/CN102515888B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001019940A1 (en) * | 1999-09-16 | 2001-03-22 | Dirk Van Barneveld | Soil improver composition and plant growth enhancer |
| RU2192403C2 (en) * | 2000-06-23 | 2002-11-10 | Иркутский институт химии СО РАН | Method of organomineral fertilizer preparing |
| CN102079675A (en) * | 2010-12-03 | 2011-06-01 | 张新雄 | Magnetic biofertilizer and preparation method |
| CN102093126A (en) * | 2010-12-03 | 2011-06-15 | 张新雄 | Method for producing bio-fertilizer from waste organic matters |
Non-Patent Citations (4)
| Title |
|---|
| Quality evaluation of co-composted wheat straw,poultry droppings and oil seed cakes;Sunita Gaind 等;《Biodegradation》;20090630;第20卷(第3期);307-317 * |
| SunitaGaind等.Qualityevaluationofco-compostedwheatstraw poultry droppings and oil seed cakes.《Biodegradation》.2009 |
| 白腐菌应用于堆肥处理含木质素废物的研究;黄丹莲 等;《环境污染治理技术与设备》;20050228;第6卷(第2期);29-32 * |
| 黄丹莲 等.白腐菌应用于堆肥处理含木质素废物的研究.《环境污染治理技术与设备》.2005,第6卷(第2期),29-32. |
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