CN102459610B - 表达pdh和fdh酶的遗传稳定性质粒 - Google Patents

表达pdh和fdh酶的遗传稳定性质粒 Download PDF

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CN102459610B
CN102459610B CN201080025346.7A CN201080025346A CN102459610B CN 102459610 B CN102459610 B CN 102459610B CN 201080025346 A CN201080025346 A CN 201080025346A CN 102459610 B CN102459610 B CN 102459610B
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fdh
nucleic acid
sequence
seq
polypeptide
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CN102459610A (zh
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J.巴希
T.弗朗西丝奇尼
S.W.刘
S-J.蒋
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AstraZeneca AB
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C12N9/0018—Phenylalanine dehydrogenase (1.4.1.20)

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Physics & Mathematics (AREA)
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  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201080025346.7A 2009-04-08 2010-04-08 表达pdh和fdh酶的遗传稳定性质粒 Active CN102459610B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16767609P 2009-04-08 2009-04-08
US61/167,676 2009-04-08
PCT/US2010/030404 WO2010118240A1 (en) 2009-04-08 2010-04-08 A genetically stable plasmid expressing pdh and fdh enzymes

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CN102459610A CN102459610A (zh) 2012-05-16
CN102459610B true CN102459610B (zh) 2014-12-17

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US (1) US8158394B2 (https=)
EP (1) EP2417260A1 (https=)
JP (2) JP2012523235A (https=)
KR (1) KR101292379B1 (https=)
CN (1) CN102459610B (https=)
SG (1) SG175054A1 (https=)
WO (1) WO2010118240A1 (https=)

Families Citing this family (8)

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CN103965065B (zh) * 2013-02-01 2016-02-17 上海现代制药股份有限公司 沙格列汀中间体、其盐、其制备方法及其用途
WO2014134281A1 (en) * 2013-02-28 2014-09-04 Full Spectrum Laboratories Limited Biosynthesis of cannabinoids
CN103555683B (zh) * 2013-11-19 2015-11-18 南京博优康远生物医药科技有限公司 一种沙格列汀手性中间体的合成方法
WO2015087262A1 (en) 2013-12-11 2015-06-18 Ranbaxy Laboratories Limited Process for the preparation of saxagliptin and its intermediates
CN103951588B (zh) * 2014-04-30 2016-10-05 淮海工学院 一种合成沙格列汀中间体n-叔丁氧羰基-3-羟基-1-金刚烷基-d-甘氨酸的方法
US9394510B2 (en) 2014-08-25 2016-07-19 Full Spectrum Laboratories Limited Apparatus and methods for the simultaneous production of compounds
CN108795893B (zh) * 2018-06-27 2021-10-08 凯莱英医药集团（天津）股份有限公司 一种氨基酸脱氢酶突变体及其制备方法和应用
CN112391363B (zh) * 2021-01-21 2021-04-06 凯莱英生命科学技术(天津)有限公司 氨基酸脱氢酶突变体及其应用

Citations (3)

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Publication number Priority date Publication date Assignee Title
US6068991A (en) * 1997-12-16 2000-05-30 Bristol-Myers Squibb Company High expression Escherichia coli expression vector
CN1791401A (zh) * 2002-12-09 2006-06-21 布里斯托尔-迈尔斯斯奎布公司 用于生产二肽基肽酶iv抑制剂和它的中间体的方法和化合物
CN1968925A (zh) * 2004-04-14 2007-05-23 布里斯托尔-迈尔斯·斯奎布公司 制备二肽基肽酶iv抑制剂的方法及用于该制备方法的中间体

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US6395767B2 (en) * 2000-03-10 2002-05-28 Bristol-Myers Squibb Company Cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV and method
US7087418B2 (en) * 2001-12-19 2006-08-08 Bristol-Myers Squibb Company Pichia pastoris formate dehydrogenase and uses therefor
US8227228B2 (en) 2005-08-02 2012-07-24 Kaneka Corporation D-amino acid oxidase, and method for production of L-amino acid, 2-oxo acid, or cyclic imine

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6068991A (en) * 1997-12-16 2000-05-30 Bristol-Myers Squibb Company High expression Escherichia coli expression vector
CN1791401A (zh) * 2002-12-09 2006-06-21 布里斯托尔-迈尔斯斯奎布公司 用于生产二肽基肽酶iv抑制剂和它的中间体的方法和化合物
US7420079B2 (en) * 2002-12-09 2008-09-02 Bristol-Myers Squibb Company Methods and compounds for producing dipeptidyl peptidase IV inhibitors and intermediates thereof
CN1968925A (zh) * 2004-04-14 2007-05-23 布里斯托尔-迈尔斯·斯奎布公司 制备二肽基肽酶iv抑制剂的方法及用于该制备方法的中间体

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from Thermoactinomyces intermedius;Ronald L. Hanson et al.;《Enzyme and Microbial Technology》;20000501;第26卷;全文 *
Synthesis of Optically Active Amino Acids from a-Keto Acids with Escherichia coli Cells Expressing Heterologous Genes;ANDREY GALKIN et al.;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;19971231;第63卷(第12期);4651、4653 *
Thermostable Phenylalanine Dehydrogenase of Thermoactinomyces intermedius: Cloning, Expression, and Sequencing of Its Gene;Harumi Takada et al.;《J. Biochem》;19910531;第109卷;全文 *
tRNA dynamics on the ribosome during translation;Scott C. Blanchard et al.;《PNAS》;20040818;第101卷(第35期);12894 *

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Publication number Publication date
KR20110135990A (ko) 2011-12-20
KR101292379B1 (ko) 2013-08-09
EP2417260A1 (en) 2012-02-15
US20100261236A1 (en) 2010-10-14
JP2014128281A (ja) 2014-07-10
CN102459610A (zh) 2012-05-16
US8158394B2 (en) 2012-04-17
WO2010118240A1 (en) 2010-10-14
SG175054A1 (en) 2011-11-28
JP2012523235A (ja) 2012-10-04

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