JP2012523235A - Ｐｄｈおよびｆｄｈ酵素を発現する遺伝学的に安定なプラスミド - Google Patents

Ｐｄｈおよびｆｄｈ酵素を発現する遺伝学的に安定なプラスミド Download PDF

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JP2012523235A
JP2012523235A JP2012504868A JP2012504868A JP2012523235A JP 2012523235 A JP2012523235 A JP 2012523235A JP 2012504868 A JP2012504868 A JP 2012504868A JP 2012504868 A JP2012504868 A JP 2012504868A JP 2012523235 A JP2012523235 A JP 2012523235A
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seq
nucleic acid
sequence
polypeptide
fdh
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JP2012523235A5 (https=
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ジョナサン・バッシュ
トーマス・フランセスキーニ
スオ・ウィン・リュー
シュ−ジェン・チャン
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Bristol Myers Squibb Co
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    • C—CHEMISTRY; METALLURGY
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    • C12N9/0004—Oxidoreductases (1.)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/68—Stabilisation of the vector
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C12N9/0018—Phenylalanine dehydrogenase (1.4.1.20)

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2012504868A 2009-04-08 2010-04-08 Ｐｄｈおよびｆｄｈ酵素を発現する遺伝学的に安定なプラスミド Pending JP2012523235A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16767609P 2009-04-08 2009-04-08
US61/167,676 2009-04-08
PCT/US2010/030404 WO2010118240A1 (en) 2009-04-08 2010-04-08 A genetically stable plasmid expressing pdh and fdh enzymes

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JP2014041887A Division JP2014128281A (ja) 2009-04-08 2014-03-04 Ｐｄｈおよびｆｄｈ酵素を発現する遺伝学的に安定なプラスミド

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JP2012523235A true JP2012523235A (ja) 2012-10-04
JP2012523235A5 JP2012523235A5 (https=) 2013-11-07

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JP2012504868A Pending JP2012523235A (ja) 2009-04-08 2010-04-08 Ｐｄｈおよびｆｄｈ酵素を発現する遺伝学的に安定なプラスミド
JP2014041887A Pending JP2014128281A (ja) 2009-04-08 2014-03-04 Ｐｄｈおよびｆｄｈ酵素を発現する遺伝学的に安定なプラスミド

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US (1) US8158394B2 (https=)
EP (1) EP2417260A1 (https=)
JP (2) JP2012523235A (https=)
KR (1) KR101292379B1 (https=)
CN (1) CN102459610B (https=)
SG (1) SG175054A1 (https=)
WO (1) WO2010118240A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018086012A (ja) * 2013-02-28 2018-06-07 ティーウィノット テクノロジーズ リミテッド カンナビノイドの生合成
US10633681B2 (en) 2014-08-25 2020-04-28 Teewinot Technologies Limited Apparatus and methods for biosynthetic production of cannabinoids

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103965065B (zh) * 2013-02-01 2016-02-17 上海现代制药股份有限公司 沙格列汀中间体、其盐、其制备方法及其用途
CN103555683B (zh) * 2013-11-19 2015-11-18 南京博优康远生物医药科技有限公司 一种沙格列汀手性中间体的合成方法
WO2015087262A1 (en) 2013-12-11 2015-06-18 Ranbaxy Laboratories Limited Process for the preparation of saxagliptin and its intermediates
CN103951588B (zh) * 2014-04-30 2016-10-05 淮海工学院 一种合成沙格列汀中间体n-叔丁氧羰基-3-羟基-1-金刚烷基-d-甘氨酸的方法
CN108795893B (zh) * 2018-06-27 2021-10-08 凯莱英医药集团（天津）股份有限公司 一种氨基酸脱氢酶突变体及其制备方法和应用
CN112391363B (zh) * 2021-01-21 2021-04-06 凯莱英生命科学技术(天津)有限公司 氨基酸脱氢酶突变体及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050090539A1 (en) * 2002-12-09 2005-04-28 Vu Truc C. Methods and compounds for producing dipeptidyl peptidase IV inhibitors and intermediates thereof
WO2005106011A2 (en) * 2004-04-14 2005-11-10 Bristol-Myers Squibb Company Process for preparing dipeptidyl iv inhibitors and intermediates therefor
EP1918375A1 (en) * 2005-08-02 2008-05-07 Kaneka Corporation D-amino acid oxidase, and method for production of l-amino acid, 2-oxo acid or cyclic imine

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6068991A (en) * 1997-12-16 2000-05-30 Bristol-Myers Squibb Company High expression Escherichia coli expression vector
US6395767B2 (en) * 2000-03-10 2002-05-28 Bristol-Myers Squibb Company Cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV and method
US7087418B2 (en) * 2001-12-19 2006-08-08 Bristol-Myers Squibb Company Pichia pastoris formate dehydrogenase and uses therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050090539A1 (en) * 2002-12-09 2005-04-28 Vu Truc C. Methods and compounds for producing dipeptidyl peptidase IV inhibitors and intermediates thereof
WO2005106011A2 (en) * 2004-04-14 2005-11-10 Bristol-Myers Squibb Company Process for preparing dipeptidyl iv inhibitors and intermediates therefor
EP1918375A1 (en) * 2005-08-02 2008-05-07 Kaneka Corporation D-amino acid oxidase, and method for production of l-amino acid, 2-oxo acid or cyclic imine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN7013001696; Ｇａｌｋｉｎ　Ａ　ｅｔ　ａｌ: 'Ｓｙｎｔｈｅｓｉｓ　ｏｆ　Ｏｐｔｉｃａｌｌｙ　Ａｃｔｉｖｅ　Ａｍｉｎｏ　Ａｃｉｄｓ　ｆｒｏｍ　ａｌｐｈａ-Ｋｅｔｏ　Ａｃｉｄｓ　ａｎｄ　Ｅｓｃｈｅｒｉｃｈｉａ　ｃｏｌｉ　Ｃｅｌｌｓ　Ｅｘｐｒｅｓｓｉｎ' Ａｐｐｌｉｅｄ　ａｎｄ　Ｅｎｖｉｒｏｎｍｅｎｔａｌ　Ｍｉｃｒｏｂｉｏｌｏｇｙ Ｖｏｌ．６３，　Ｎｏ．１２，, 1997, ｐ．４６５１-４６５６ *
JPN7013001698; Ｋｉｍｕｒａ　Ｓ　ｅｔ　ａｌ: Ｊ．　Ｂｉｏｃｈｅｍ． Ｖｏｌ．１３７，　Ｎｏ．４, 2005, ｐ．５２３-５３３ *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018086012A (ja) * 2013-02-28 2018-06-07 ティーウィノット テクノロジーズ リミテッド カンナビノイドの生合成
US10472652B2 (en) 2013-02-28 2019-11-12 Teewinot Technologies Limited Chemical engineering processes and apparatus for the synthesis of compounds
US10633681B2 (en) 2014-08-25 2020-04-28 Teewinot Technologies Limited Apparatus and methods for biosynthetic production of cannabinoids

Also Published As

Publication number Publication date
KR20110135990A (ko) 2011-12-20
KR101292379B1 (ko) 2013-08-09
EP2417260A1 (en) 2012-02-15
US20100261236A1 (en) 2010-10-14
JP2014128281A (ja) 2014-07-10
CN102459610B (zh) 2014-12-17
CN102459610A (zh) 2012-05-16
US8158394B2 (en) 2012-04-17
WO2010118240A1 (en) 2010-10-14
SG175054A1 (en) 2011-11-28

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