CN102405858A - Artificial incubation and culture method for opsariichthys bidens - Google Patents
Artificial incubation and culture method for opsariichthys bidens Download PDFInfo
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- CN102405858A CN102405858A CN2011102668930A CN201110266893A CN102405858A CN 102405858 A CN102405858 A CN 102405858A CN 2011102668930 A CN2011102668930 A CN 2011102668930A CN 201110266893 A CN201110266893 A CN 201110266893A CN 102405858 A CN102405858 A CN 102405858A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides relates to an artificial incubation and culture method for wild fish in rivers and particularly relates to an artificial incubation and culture method for opsariichthys bidens. The method comprises the following steps: parent fish culture, parent fish selective pairing, artificial inducement for spawning, artificial insemination, roe incubation, offspring seed culture and cage culture. According to the invention, the artificial incubation and artificial culture of the opsariichthys bidens are successfully realized; and the method has the characteristics of high fertilizing rate, high hatchability, high survival rate and high yield, and has important significance in restoring and developing wild fish resources in rivers.
Description
Technical field
The present invention relates to the artificial incubation and the cultural method of artificial incubation and the cultural method, particularly opsariichthys bidens of a kind of wild little freshwater fish.
Background technology
Opsariichthys bidens is also claimed southern opsariichthys bidens, and it is public to be commonly called as May green or screw oil expeller, is a kind of wild fish of living in the good river of the water quality brook, inhabits the upper strata, waters; Happiness low temperature water quality is predacious fish, and property is violent, is food with small fish and aquatic insect; Though maximum individuality has only 20cm, nutritive value is very abundant, delicious flavour, quite gets consumer reception; Now, wild fish is fewer and feweri, has been difficult on the market see.Because the opsariichthys bidens growth conditions is harsh, does not also have artificial incubation at present and culture successful report.
Summary of the invention
The objective of the invention is to disclose a kind of artificial incubation and cultural method of opsariichthys bidens.Technical solution of the present invention is: the artificial incubation of opsariichthys bidens and cultural method are carried out according to the following steps:
A. parent fish rearing: catch wild opsariichthys bidens and put into the parent fish rearing case, the small fish shrimp of throwing something and feeding every day carries out reinforced cultivating;
B. parent population apolegamy: by the opsariichthys bidens of the ratio selective maturation of male and female proportioning 1:1-1:20 as the breed parent population;
C. artificial induced spawning: water temperature is controlled at 20-26 ℃; Parent population is carried out artificial induced spawning; The medicine of promoting the sexual maturity adopts DOPA ammonium D-2 receptor-specific antagonist DOM adjunction ovum plain No. 2 (LRH-Ar) to mix and uses; In the disposable injection of pectoral fin base portion, its dosage is by per kilogram of body weight 0.5-10mg, puts into net cage after the injection and observes till begin to chase;
D. artificial insemination: will reach the parent population that chases effect and take out, and carry out artificial insemination;
E.. natural spawning: the fertilized egg of nature output is put into hatching pail or incubator box is hatched;
F. hatch fish roe: the ovum that artificial insemination obtains is hatched in hatching pail, put 4-40 ten thousand on ovum for every barrel, uninterrupted water-filling; Perhaps hatch: put ovum amount≤200,000 for every cubic metre, put 5-8 gas charging stones in the case, uninterrupted inflation with net cage; Prevent that the ovum grain from sinking to the bottom, in hatching process, constantly observe the embryonic development situation of fish-egg, kill star 5-15mg/kg sterilization 10-60 minute with water mold every day between the incubation period; Prevent that water mold from taking place, the hatching water temperature is 22-26 ℃;
G. seed rearing: put into the seed rearing case when fry that hatches begins to have the swimming ability or cultivate the pond and cultivate; Open-mouthed bait is that wheel animalcule and small-sized cladocera are thrown something and fed; The big cladocera of after the increase of individuality, throwing something and feeding again; Through cultivating, become the commodity fry or change adult fish cage culture over to after fry body length on average reaches 1.5-2.5cm;
H. cage culture:: use the adult fish net cage, breed fish fry≤30,000 tails, and carry out the granular bait for fish domesticating and cultivating by cubic meter; Begin to be equipped with the granule feed of throwing something and feeding, stop to feed a branch angle after 10-12 days fully by day feeding volume of minimizing cladocera; Change granule bait into, beginning is is repeatedly thrown something and fed on a small quantity, and throw something and feed 4-8 every day; Day, feeding volume was 3-20% of a body weight, and adult fish is expected in progressively throwing something and feeding after 10-20 days again, and then the adult fish material of throwing something and feeding is until the fish outlet.
Owing to adopted artificial incubation that above technical scheme, the present invention successfully realized opsariichthys bidens and propagated artificially, have fertilization rate, incubation rate, characteristics that survival rate is high.
Embodiment
Below in conjunction with embodiment; The present invention is further described. embodiment 1: a kind of artificial incubation of opsariichthys bidens and cultural method are carried out according to the following steps: a. parent fish rearing: catch wild opsariichthys bidens and put into the parent fish rearing case; The small fish shrimp of throwing something and feeding every day carries out reinforced cultivating; B. parent population apolegamy: by the opsariichthys bidens of the ratio selective maturation of male and female proportioning 1:1 as the breed parent population; C. artificial induced spawning: water temperature is controlled at 22 ℃; Parent population is carried out artificial induced spawning; The medicine of promoting the sexual maturity adopts DOPA ammonium D-2 receptor-specific antagonist DOM adjunction ovum plain No. 2 (LRH-Ar) to mix and uses; In the disposable injection of pectoral fin base portion, its dosage is by per kilogram of body weight 5mg, puts into net cage after the injection and observes till begin to chase; D. artificial insemination: will reach the parent population that chases effect and take out, and carry out artificial insemination; E.. natural spawning: the fertilized egg of nature output is put into hatching pail or incubator box is hatched; F. hatch fish roe: the ovum that artificial insemination obtains is hatched in hatching pail, put 40,000 on ovum for every barrel, uninterrupted water-filling; In hatching process, constantly observe the embryonic development situation of fish-egg, kill star 5mg/kg sterilization 10 minute with water mold every day between the incubation period, prevents that water mold from taking place; The hatching water temperature is 22 ℃; Perhaps hatch: put 5-20 ten thousand on ovum, put 5-8 gas charging stones for every cubic metre, continual inflation for every cubic metre with net cage; G. seed rearing: put into the seed rearing case when fry that hatches begins to have the swimming ability and cultivate; The seed rearing cage size is 1m * 1m * 2m; Every case is put 30,000 tails in a suitable place to breed; Open-mouthed bait is that wheel animalcule and small-sized cladocera are thrown something and fed, and along with the increase of the individuality big cladocera of can only throwing something and feeding, feeding volume is looked the situation of ingesting and decided; Through cultivating, after on average reaching 1.5-2.5cm, fry body length changes adult fish cage culture over to; H. the adult fish net cage of cage culture: 2m * 2m * 2m, every case 1.5 ten thousand tails that breed fish fry, and carry out the granular bait for fish domesticating and cultivating; Begin to be equipped with the granule feed of throwing something and feeding, stop to feed a branch angle after 10 days fully by day feeding volume of minimizing cladocera; Change granule bait into, beginning is is repeatedly thrown something and fed on a small quantity, throws something and feeds every day 5 times; Day feeding volume is an adult fish material in progressively throwing something and feeding again behind 5%, 18 day of body weight, and then the adult fish material of throwing something and feeding is until the fish outlet.Consequently; Fertilization rate is 75%, and incubation rate is 66%, survival rate of fish fry 58%, the adult fish survival rate be 67%, three monthly age adult fish average body length with 9cm, average cabrage 35g.
Embodiment 2:a. parent fish rearing: catch wild opsariichthys bidens and put into the parent fish rearing case, the small fish shrimp of throwing something and feeding every day carries out reinforced cultivating; B. parent population apolegamy: by the opsariichthys bidens of the ratio selective maturation of male and female proportioning 1:10 as the breed parent population; C. artificial induced spawning: water temperature is controlled at 24 ℃; Parent population is carried out artificial induced spawning; The medicine of promoting the sexual maturity adopts DOPA ammonium D-2 receptor-specific antagonist DOM adjunction ovum plain No. 2 (LRH-Ar) to mix and uses; In the disposable injection of pectoral fin base portion, its dosage is by per kilogram of body weight 8mg, puts into net cage after the injection and observes till begin to chase; D. artificial insemination: will reach the parent population that chases effect and take out, and carry out artificial insemination; E. hatch fish roe: the ovum that artificial insemination obtains is hatched in hatching pail; Put 50,000 on ovum for every barrel; Uninterrupted water-filling, the embryonic development situation of in hatching process, constantly observing fish-egg, kill star 10mg/kg sterilization 20 minute with water mold every day between the incubation period; Prevent that water mold from taking place, the hatching water temperature is 24 ℃; F. seed rearing: put into the seed rearing case when fry that hatches begins to have the swimming ability and cultivate; The seed rearing cage size is 1m * 1m * 2m, and every case is put 20,000 tails in a suitable place to breed, and the specification of cultivating net cage is changeable; Stocking rate is by every cubic metre of 0.1-3 ten thousand tails; Open-mouthed bait is that wheel animalcule and small-sized cladocera are thrown something and fed, and along with the increase of the individuality big cladocera of can only throwing something and feeding, feeding volume is looked the situation of ingesting and decided; Through cultivating, after on average reaching 2cm, fry body length changes adult fish cage culture over to; G. the adult fish net cage of cage culture: 2m * 2m * 2m, every case 1.8 ten thousand tails that breed fish fry, and carry out the granular bait for fish domesticating and cultivating; Begin to be equipped with the granule feed of throwing something and feeding, stop to feed a branch angle after 11 days fully by day feeding volume of minimizing cladocera; Change granule bait into, beginning is is repeatedly thrown something and fed on a small quantity, throws something and feeds every day 6 times; Day feeding volume is an adult fish material in progressively throwing something and feeding again behind 10%, 20 day of body weight, and then the adult fish material of throwing something and feeding is until the fish outlet.Consequently; Fertilization rate is 80%, and incubation rate is 70%, survival rate of fish fry 60%, the adult fish survival rate be 70%, three monthly age adult fish average body length with 10cm, average cabrage 40g.
Embodiment 3:a. parent fish rearing: catch wild opsariichthys bidens and put into the parent fish rearing case, the small fish shrimp of throwing something and feeding every day carries out reinforced cultivating; B. parent population apolegamy: by the opsariichthys bidens of the ratio selective maturation of male and female proportioning 1:20 as the breed parent population; C. artificial induced spawning: water temperature is controlled at 26 ℃; Parent population is carried out artificial induced spawning; The medicine of promoting the sexual maturity adopts DOPA ammonium D-2 receptor-specific antagonist DOM adjunction ovum plain No. 2 (LRH-Ar) to mix and uses; In the disposable injection of pectoral fin base portion, its dosage is by per kilogram of body weight 10mg, puts into net cage after the injection and observes till begin to chase; D. artificial insemination: will reach the parent population that chases effect and take out, and carry out artificial insemination; E. hatch fish roe: the ovum that artificial insemination obtains is hatched in hatching pail; Put 80,000 on ovum for every barrel; Uninterrupted water-filling, the embryonic development situation of in hatching process, constantly observing fish-egg, kill star 15mg/kg sterilization 40-60 minute with water mold every day between the incubation period; Prevent that water mold from taking place, the hatching water temperature is 26 ℃; F. seed rearing: put into the seed rearing case when fry that hatches begins to have the swimming ability and cultivate; The seed rearing cage size is 1m * 1m * 2m; Every case is put 100,000 tails in a suitable place to breed; Open-mouthed bait is that wheel animalcule and small-sized cladocera are thrown something and fed, and along with the increase of the individuality big cladocera of can only throwing something and feeding, feeding volume is looked the situation of ingesting and decided; Through cultivating, after on average reaching 1.8-2cm, fry body length changes adult fish cage culture over to; G. the adult fish net cage of cage culture: 2m * 2m * 2m, every case 30,000 tails that breed fish fry, and carry out the granular bait for fish domesticating and cultivating; Begin to be equipped with the granule feed of throwing something and feeding, stop to feed a branch angle after 12 days fully by day feeding volume of minimizing cladocera; Change granule bait into, beginning is is repeatedly thrown something and fed on a small quantity, throws something and feeds every day 8 times; Day, feeding volume was 15% of a body weight, and adult fish is expected in progressively throwing something and feeding after about 20 days again, and then the adult fish material of throwing something and feeding is until the fish outlet.Consequently; Fertilization rate is 72%, and incubation rate is 65%, survival rate of fish fry 55%, the adult fish survival rate be 68%, three monthly age adult fish average body length with 8.5cm, average cabrage 32g.
Embodiment 4: hatch fish roe: the ovum that artificial insemination obtains is hatched in incubator box, and incubator box is put ten thousand of ovum 1-40 for every cubic metre, ten thousand of best 15-20, and uninterrupted inflation, other is with embodiment 3.
The mesh of the hatching net cage among the above embodiment is generally 40 orders, and the mesh of fish culture net cage is generally 0.5cm.
Claims (9)
1. the artificial incubation of opsariichthys bidens and cultural method is characterized in that carrying out according to the following steps:
A. parent fish rearing: catch wild opsariichthys bidens and put into the parent fish rearing case, the small fish shrimp of throwing something and feeding every day carries out reinforced cultivating;
B. parent population apolegamy: by the opsariichthys bidens of the ratio selective maturation of male and female proportioning 1:1-1:20 as the breed parent population;
C. artificial induced spawning: water temperature is controlled at 20-26 ℃; Parent population is carried out artificial induced spawning; The medicine of promoting the sexual maturity adopts DOPA ammonium D-2 receptor-specific antagonist DOM adjunction ovum plain No. 2 (LRH-Ar) to mix and uses; In the disposable injection of pectoral fin base portion, its dosage is by per kilogram of body weight 0.5-10mg, puts into net cage after the injection and observes till begin to chase;
D. artificial insemination: will reach the parent population that chases effect and take out, and carry out artificial insemination;
E.. natural spawning: the fertilized egg of nature output is put into hatching pail or incubator box is hatched;
F. hatch fish roe: the ovum that artificial insemination obtains is hatched in hatching pail, put 4-40 ten thousand on ovum for every barrel, uninterrupted water-filling; Perhaps hatch: put ovum amount≤200,000 for every cubic metre, put 5-8 gas charging stones in the case, uninterrupted inflation with net cage; Prevent that the ovum grain from sinking to the bottom, in hatching process, constantly observe the embryonic development situation of fish-egg, kill star 5-15mg/kg sterilization 10-60 minute with water mold every day between the incubation period; Prevent that water mold from taking place, the hatching water temperature is 22-26 ℃;
G. seed rearing: put into the seed rearing case when fry that hatches begins to have the swimming ability or cultivate the pond and cultivate; Open-mouthed bait is that wheel animalcule and small-sized cladocera are thrown something and fed; The big cladocera of after the increase of individuality, throwing something and feeding again; Through cultivating, the fry body is long on average to be reached to become the commodity fry or change the adult fish net cage over to behind the 1.5-2.5cm and propagates artificially.
2. the artificial incubation of opsariichthys bidens according to claim 1 and cultural method is characterized in that said propagating artificially, are the opsariichthys bidens fry that artificial incubation is cultivated is put into the adult fish net cage; Every cubic metre of net cage breeds fish fry≤30,000 tails, and carries out the granular bait for fish domesticating and cultivating, begins by day feeding volume of minimizing cladocera; Be equipped with the granule feed of throwing something and feeding, stop to feed the branch angle after 10-12 days fully, change granule bait into; Beginning is is repeatedly thrown something and fed on a small quantity, and throw something and feed 4-8 every day, and a day feeding volume is 3-20% of a body weight; Adult fish material in progressively throwing something and feeding again after 10-20 days, and then the adult fish material of throwing something and feeding is until the fish outlet.
3. the artificial incubation of opsariichthys bidens according to claim 1 and cultural method is characterized in that said parent population apolegamy carries out proportioning in the ratio of male and female 1:1-1:2.
4. the artificial incubation of opsariichthys bidens according to claim 1 and cultural method is characterized in that the water temperature of said artificial induced spawning is controlled at 23-26 ℃.
5. the artificial incubation of opsariichthys bidens according to claim 1 and cultural method; It is characterized in that said hatch fish roe is that the ovum that artificial insemination obtains is hatched in hatching pail, put 10-20 ten thousand on ovum for every barrel, uninterrupted water-filling; In hatching process, constantly observe the embryonic development situation of fish-egg; Kill star 10mg/kg sterilization 20 minute with water mold every day between the incubation period, prevents that water mold from taking place, and the hatching water temperature is 23-26 ℃.
6. the artificial incubation of opsariichthys bidens according to claim 1 and cultural method; It is characterized in that said hatch fish roe is that the ovum that artificial insemination obtains is hatched in net cage; Put ovum amount≤200,000 for every cubic metre; Put 5-8 gas charging stones in the case, uninterrupted inflation prevents that the ovum grain from sinking to the bottom..
7. the artificial incubation of opsariichthys bidens according to claim 1 and cultural method is characterized in that said seed rearing, put in a suitable place to breed≤50,000 tails for every cubic metre.
8.. the artificial incubation of opsariichthys bidens according to claim 2 and cultural method is characterized in that said propagating artificially, are the opsariichthys bidens fry that artificial incubation is cultivated is put into the adult fish net cage; Every cubic metre of adult fish net cage 0.1-2 ten thousand tails that breed fish fry, and carry out the granular bait for fish domesticating and cultivating begin by a feeding volume that day reduces cladocera; Be equipped with the granule feed of throwing something and feeding, stop to feed the branch angle after 10 days fully, change granule bait into; Beginning is is repeatedly thrown something and fed on a small quantity, throws something and feeds every day 6 times, and a day feeding volume is the 10-20% of body weight; Adult fish material in progressively throwing something and feeding again after 18-20 days, and then the adult fish material of throwing something and feeding is until the fish outlet.
9.. the artificial incubation of opsariichthys bidens according to claim 2 and cultural method is characterized in that said propagating artificially, are the opsariichthys bidens fry that artificial incubation is cultivated is put into the adult fish net cage; Every cubic metre of adult fish net cage 0.1-2 ten thousand tails that breed fish fry, and carry out the granular bait for fish domesticating and cultivating begin by a feeding volume that day reduces cladocera; Be equipped with the granule feed of throwing something and feeding, stop to feed the branch angle after 10 days fully, change granule bait into; Beginning is is repeatedly thrown something and fed on a small quantity, throws something and feeds every day 6 times, and a day feeding volume is the 10-20% of body weight; Adult fish material in progressively throwing something and feeding again after 18-20 days, and then the adult fish material of throwing something and feeding is until the fish outlet.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105394446A (en) * | 2015-11-16 | 2016-03-16 | 郎溪县飞鲤镇幸福好运家畜养殖家庭农场 | Feed for young opsariichthys bidens |
| CN107751048A (en) * | 2017-10-20 | 2018-03-06 | 浏阳神泉生态水产养殖专业合作社 | A kind of flowing water pool cultivated method of southern opsariichthys bidenses |
| CN107821247A (en) * | 2017-11-29 | 2018-03-23 | 宁波大学 | A kind of whole artificial propagation method of opsariichthys bidenses |
| CN113699256A (en) * | 2021-09-26 | 2021-11-26 | 浙江省农业科学院 | Functional marker for rapidly identifying gynogenesis progeny of opsariichthys bidens and fancy carps and application of functional marker |
| CN115735810A (en) * | 2022-11-07 | 2023-03-07 | 华中农业大学 | Method for cultivating full-male opsariichthys bidens with high growth speed |
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2011
- 2011-09-09 CN CN2011102668930A patent/CN102405858A/en active Pending
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105394446A (en) * | 2015-11-16 | 2016-03-16 | 郎溪县飞鲤镇幸福好运家畜养殖家庭农场 | Feed for young opsariichthys bidens |
| CN107751048A (en) * | 2017-10-20 | 2018-03-06 | 浏阳神泉生态水产养殖专业合作社 | A kind of flowing water pool cultivated method of southern opsariichthys bidenses |
| CN107821247A (en) * | 2017-11-29 | 2018-03-23 | 宁波大学 | A kind of whole artificial propagation method of opsariichthys bidenses |
| CN113699256A (en) * | 2021-09-26 | 2021-11-26 | 浙江省农业科学院 | Functional marker for rapidly identifying gynogenesis progeny of opsariichthys bidens and fancy carps and application of functional marker |
| CN113699256B (en) * | 2021-09-26 | 2023-06-20 | 浙江省农业科学院 | Functional marker for rapidly identifying gynogenesis offspring of salmon and koi and application of functional marker |
| CN115735810A (en) * | 2022-11-07 | 2023-03-07 | 华中农业大学 | Method for cultivating full-male opsariichthys bidens with high growth speed |
| CN115735810B (en) * | 2022-11-07 | 2024-05-03 | 华中农业大学 | Method for cultivating all-male salmon with high growth speed |
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Application publication date: 20120411 |