CN102369874A - Method for carrying out differentiating, multiplication, rooting and stock exercising on tissue culture seedlings cultured outside test tubes - Google Patents
Method for carrying out differentiating, multiplication, rooting and stock exercising on tissue culture seedlings cultured outside test tubes Download PDFInfo
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- CN102369874A CN102369874A CN201110249124XA CN201110249124A CN102369874A CN 102369874 A CN102369874 A CN 102369874A CN 201110249124X A CN201110249124X A CN 201110249124XA CN 201110249124 A CN201110249124 A CN 201110249124A CN 102369874 A CN102369874 A CN 102369874A
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Abstract
The invention discloses a method for carrying out differentiating, multiplication, rooting and stock exercising on tissue culture seedlings cultured outside test tubes, which belongs to a technique for stock growing. The method is characterized in that a culture vessel comprises a grid baseplate and a side plate, wherein the binding position of the grid baseplate and the side plate is higher than the lower edge of the side plate, the lower edge of the side plate is provided with a compact baseplate which is hermetically bound with the surrounding edges of the side plate, a part (below the binding position of the grid baseplate and the side plate) of the side plate is provided with an input pipe and an output pipe, an upper opening of a tray is movably combined with a stock-raising tray (for totally closed micro-environment control) with an upper cover, and the internal surface of the upper cover is provided with a spraying device and a lighting device. Compared with the prior art, the culture vessel is simple and practical, and has low manufacturing cost and use cost; the method is suitable to be used for carrying out effective control on micro environments in the differentiating, multiplication, rooting and stock exercising stages of various tissue culture seedlings cultured outside test tubes; a system for carrying out effective control on differentiating, multiplication, rooting and stock exercising micro-environments for tissue culture seedlings cultured outside test tubes can be formed by respectively connecting the input pipes and output pipes of multiple culture vessels and forming a pipe network, thereby realizing large-scale factory operating and production.
Description
Technical field: the invention belongs to plant regeneration field, the differentiation and proliferation of the outer tissue cultivating seedling of the particularly a kind of test tube hardening off method of taking root through tissue culture technique.
Background technology: start " test-tube plantlet " heat at present both at home and abroad, many flowers, forest, fruit tree, vegetables all can carry out large-scale vegetative propagation through tissue culture.Owing to can carry out the cultivation of virus-free plant through tissue culture; And all be under the environmental condition of artificial sterile working, to carry out large-scale artificial culture and batch production production; Therefore tissue culture technique has special role to the pure and mild anti-season production of guaranteeing the quality, protect of food resource, is expected to will form big industry in this century.Present various tissue cultivating and seedling technology; The differentiation and proliferation of the outer tissue cultivating seedling of the test tube refining seedling of taking root is all adopted the triangle vial; Adopt this culture vessel, it is big to exist the space that blake bottle occupies, and transport difficulty is high; The transport space that its empty bottle and seedling bottle occupy when transportation is big, can cause bigger transportation loss simultaneously; Have vial to be difficult to clean again, manpower that expends and expense have increased the production cost; The differentiation and proliferation of the outer tissue cultivating seedling of the test tube culture environment such as the required temperature of refining seedling process, illumination of taking root all must rely on the equipment of being correlated with to realize in the parking space blake bottle outside in addition.
This shows that study and design the simple and easy practicality of a kind of culture vessel, manufacturing cost and use cost is low, the differentiation and proliferation that is fit to the outer tissue cultivating seedling of test tube that the scale batch production the produces hardening off method of taking root is necessary.
Summary of the invention: the objective of the invention is to overcome the deficiency of prior art, study and design the simple and easy practicality of a kind of culture vessel, manufacturing cost and use cost is low, the differentiation and proliferation that is fit to the outer tissue cultivating seedling of test tube that the scale batch production the produces hardening off method of taking root.
The present invention seeks to realize like this: the differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube hardening off method of taking root; It is characterized in that the culture vessel employing comprises hollow out grid base plate; Along the base plate edge and the side plate vertical with base plate; It is characterized in that the binding site of grid base plate and side plate is higher than the lower limb of side plate; The lower limb of side plate is designed with and its edge sealing closely knit base plate that combines, no hollow out grid, side plate be positioned at grid base plate binding site under be designed with input pipe and efferent duct, pallet is suitable for reading to be agreed with versatilely to have and goes up the totally-enclosed microenvironment of covering and control with the pallet of growing seedlings.
Described input pipe and efferent duct are being provided with the position not on the same side on the side plate, and input pipe the position that is provided with that the position is higher than efferent duct is set.All be designed with the check valve that control inputs or outputs on described input pipe and the efferent duct.The technique effect of above-mentioned design is; From be input into, efferent duct is carried moisture, nutriment and other promote root germinating, the material of growing, the ratio of transfer oxygen and carbon dioxide, temperature, humidity, more evenly reasonable.The design of check valve is in case of necessity behind the valve-off, makes relative closure below the grid base plate of the pallet of growing seedlings, to build and to control the differentiation and proliferation microenvironment below the refining outer tissue cultivating seedling root of test tube in seedling stage of taking root better.Described input pipe is arranged at the side plate bottom near closely knit base plate.The technique effect of this design is that the moisture that it(?) in case of necessity can emptying gets into from input pipe or infiltrate from culture vessel top, nutriment and other promote root germinating, the material of growing.
The described end opening of going up cover and pallet be suitable for reading to be agreed with versatilely and is through being designed with the concavo-convex tenon effected that interlocks.The described inner face of going up cover is designed with sprayer unit and illumination apparatus.The technique effect of above-mentioned design is: the end opening that covers on the concavo-convex tenon effected that interlocks and pallet is suitable for reading agrees with versatilely; The outer tissue cultivating seedling microenvironment of test tube that one side is taken root the differentiation and proliferation on the top of the pallet of growing seedlings and refined seedling stage realizes sealing preferably; Cooperate aforementioned closing in case of necessity on input pipe and the efferent duct behind the valve; Make the microenvironment relative closure below the grid base plate of the pallet of growing seedlings, so just formed the outer tissue cultivating seedling of test tube that differentiation and proliferation the takes root refining seedling stage better whole microenvironment of growing seedlings of sealing from top to bottom.The design of sprayer unit and illumination apparatus is effective control of the microenvironment key element that requires of temperature, humidity and the illumination of the outer tissue cultivating seedling of test tube realizing various different different cultivars differentiation and proliferations are taken root refining seedling stage; On the other hand; Agree with versatilely that mean can be from the pallet of the growing seedlings cover of removing suitable for reading; The outer tissue cultivating seedling of test tube easily the differentiation and proliferation in the pallet of growing seedlings is taken root refining seedling stage is managed or is transplanted, and also can be easily sprayer unit and illumination apparatus is safeguarded or is repaired.
Technical scheme of the present invention is implemented through preliminary, has compared with prior art shown following beneficial effect:
1, the simple and easy practicality of culture vessel, manufacturing cost and use cost is low.Compare with the triangle vial that prior art adopts, only need to adopt the plastic process manufacturing, its transportation is compared big reduction with the consume in using with the glass blake bottle.
2, the totally-enclosed microenvironment that adapts to the outer tissue cultivating seedling of test tube that various different cultivars differentiation and proliferations are taken root refining seedling stage control effectively.A kind of totally-enclosed microenvironment control that the present invention implements to obtain is with the pallet of growing seedlings, and at first through input pipe input steam, microenvironment and the differentiation and proliferation medium that refines seedling of taking root carried out disinfection; Secondly the pallet of growing seedlings suitable for reading agree with versatilely have band sprayer unit and illumination apparatus on cover, realize take root effective control of the microenvironment key element that temperature, humidity, the illumination of refining seedling stage requires of differentiation and proliferation.
3, as a kind of system unit that the outer tissue cultivating seedling growth of test tube microenvironment is control effectively; Only need the input pipe and the efferent duct of numerous culture vessels of the present invention are connected into pipe network respectively, just can be combined into a system that the outer tissue cultivating seedling growth of test tube microenvironment is control effectively.On this basis, according to the outer tissue cultivating seedling of test tube being carried out by stages and in groups the differentiation and proliferation refining seedling needs partition management of taking root, just realize easily that the scale batch production of the outer tissue cultivating seedling of test tube is managed and produced.
Description of drawings: Fig. 1 is the sketch map that meets culture vessel thematic structure of the present invention; Fig. 2 is the local enlarged diagram of the tenon mouth structure among Fig. 1; Fig. 3 is the structure perspective diagram of culture vessel of the present invention.
Embodiment: the totally-enclosed microenvironment control of culture vessel of the present invention is explained as follows with the implementation detail of the pallet of growing seedlings below in conjunction with accompanying drawing:
Like Fig. 1,2, shown in 3; The differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube hardening off method of taking root; It is characterized in that the culture vessel employing comprises hollow out grid base plate 1; Along the base plate edge and the side plate 2 vertical with base plate, it is characterized in that the binding site 3 of grid base plate and side plate is higher than the lower limb 21 of side plate, the lower limb of side plate is designed with and its edge sealing closely knit base plate 4 that combine, no hollow out grid; The bottom that side plate is positioned at grid base plate binding site is designed with input pipe 5 and efferent duct 6, and suitable for reading the agreeing with versatilely of pallet has the totally-enclosed microenvironment control of going up cover 7 with the pallet of growing seedlings.In conjunction with practical implementation, also shown among Fig. 1 and grown seedlings with matrix 11 and seedling phase nursery stock 12.
Like Fig. 1, shown in 3, described input pipe 5 and efferent duct 6 are being provided with the position not on the same side on the side plate, and input pipe the position that is provided with that the position is higher than efferent duct is set, described input pipe 5 is arranged at side plate 2 bottoms near closely knit base plate 4.All be designed with the check valve 51,61 that control inputs or outputs on described input pipe 5 and the efferent duct 6.
Like Fig. 1, shown in 2, the described end opening of going up cover 7 and pallet be suitable for reading to be agreed with and is through being designed with the concavo-convex tenon mouth that interlocks 8 realizations.The described inner face of going up cover 7 is designed with sprayer unit 9.The described inner face of going up cover also is designed with illumination apparatus 10.
The differentiation and proliferation of the outer tissue cultivating seedling of the test tube of embodiment floral leaf sweet-scented oleander the hardening off method of taking root
Step 1: the bud seedling that will from test tube, grow up to; Shear; Transfer and in controlling with the pallet of growing seedlings through the totally-enclosed microenvironment that contains proliferated culture medium of sterilization, carry out enrichment culture, described proliferated culture medium is: every liter of minimal medium adds: zeatin (ZT) 0.5-1.5 milligram, 6 one benzyl purine 1.0-2.0 milligrams, indolebutyric acid 0.05-0.2 milligram and active carbon (Charcoal) 1000-2000 milligram; Step 2: with the bud seedling of cultivating in the step 1; Shear; Be inoculated in the totally-enclosed microenvironment that contains the strong seedling culture base of sterilization is controlled with the pallet of growing seedlings; Carry out strong seedling culture, get into next step after 30 days, described strong seedling culture base is: every liter of minimal medium adds: zeatin (ZT) 0.2-1.0 milligram, 6-benzyl purine 0.5-1.0 milligram, indolebutyric acid 0.1-0.3 milligram and active carbon (Charcoal) 1000-2000 milligram; Step 3: with the strong sprout of cultivating in the step 2; Remove base portion callus and partial blade; Stay 4-5 sheet blade; Be inoculated in through containing of sterilization take root handle to produce the totally-enclosed microenvironment control of root original hase with the pallet of growing seedlings in, carried out culture of rootage 7-12 days, describedly take root that to handle generation root original hase medium be that every liter of minimal medium adds: 0c-methyl (NAA) 0.2-0.5 milligram, indolebutyric acid 0.4-0.8 milligram and active carbon (Charcoal) 2000-3000 milligram; Step 4: with the unrooted seedling that has the root original hase of growth in the step 3, take out and clean, be transplanted to and contain peat: in the matrix of totally-enclosed microenvironment control with the pallet of growing seedlings of yellow soil=1: 1 (volume ratio); Water permeable; Keep 25-28 ℃ of temperature, relative moisture is more than 85%, shading (preceding 10 days) 75%; After see light gradually, take root into alive after 25 days; Step 5: have the totally-enclosed microenvironment control of band root seedling to remove growth in the step 4, let band root seedling under natural environment, grow and refined seedling in 15 days, transplant to cultivation field then with the cover of going up of the pallet of growing seedlings.
Claims (6)
1. the differentiation and proliferation of the outer tissue cultivating seedling of the test tube hardening off method of taking root; It is characterized in that the culture vessel employing comprises hollow out grid base plate (1); Along the base plate edge and the side plate (2) vertical with base plate; The binding site of grid base plate and side plate (3) is higher than the lower limb (21) of side plate; The lower limb of side plate is designed with and its edge sealing closely knit base plate that combines, no hollow out grid (4), and the bottom that side plate is positioned at grid base plate binding site is designed with input pipe (5) and efferent duct (6), and pallet is suitable for reading to be agreed with the totally-enclosed microenvironment of covering (7) on having versatilely and control with the pallet of growing seedlings.
2. the differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube according to claim 1 hardening off method of taking root; It is characterized in that described input pipe (5) and efferent duct (6) are being provided with the position not on the same side on the side plate; And input pipe the position that is provided with that the position is higher than efferent duct is set, described input pipe (5) is arranged at side plate (2) bottom near closely knit base plate (4).
3. the differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube according to claim 1 and 2 hardening off method of taking root is characterized in that all being designed with on described input pipe (5) and the efferent duct (6) and controls check valve (51), (61) that input or output.
4. the differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube according to claim 1 hardening off method of taking root is characterized in that the described end opening of going up cover (7) is through being designed with concavo-convex tenon mouth (8) realization that interlocks with suitable for reading the agreeing with of pallet.
5. the differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube according to claim 1 hardening off method of taking root is characterized in that the described inner face of going up cover (7) is designed with sprayer unit (9).
6. the differentiation and proliferation of the outer tissue cultivating seedling of a kind of test tube according to claim 1 hardening off method of taking root is characterized in that the described inner face of going up cover (7) is designed with illumination apparatus (10).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110249124XA CN102369874B (en) | 2011-08-29 | 2011-08-29 | Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110249124XA CN102369874B (en) | 2011-08-29 | 2011-08-29 | Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes |
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| CN102369874A true CN102369874A (en) | 2012-03-14 |
| CN102369874B CN102369874B (en) | 2013-03-20 |
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| CN201110249124XA Expired - Fee Related CN102369874B (en) | 2011-08-29 | 2011-08-29 | Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975832A (en) * | 2014-01-28 | 2014-08-13 | 华南农业大学 | Out-of-tube rooting method for tissue culture seedlings of silver birch |
| CN106258900A (en) * | 2016-08-26 | 2017-01-04 | 程依清 | A kind of method of Intelligent culture culture in glassware plant |
Citations (5)
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| KR100859599B1 (en) * | 2007-11-02 | 2008-09-23 | 주식회사 한테크 | Vinyl house providing a temperature control and ventilating |
| JP2009119357A (en) * | 2007-11-14 | 2009-06-04 | Fulta Electric Machinery Co Ltd | Centrifugal atomization humidifier apparatus |
| CN201345844Y (en) * | 2008-11-24 | 2009-11-18 | 孟树新 | Spraying plant-cultivating device |
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN101982061A (en) * | 2010-08-30 | 2011-03-02 | 陈胜利 | Blanching culture method of box cultured chicory |
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2011
- 2011-08-29 CN CN201110249124XA patent/CN102369874B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100859599B1 (en) * | 2007-11-02 | 2008-09-23 | 주식회사 한테크 | Vinyl house providing a temperature control and ventilating |
| JP2009119357A (en) * | 2007-11-14 | 2009-06-04 | Fulta Electric Machinery Co Ltd | Centrifugal atomization humidifier apparatus |
| CN201345844Y (en) * | 2008-11-24 | 2009-11-18 | 孟树新 | Spraying plant-cultivating device |
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN101982061A (en) * | 2010-08-30 | 2011-03-02 | 陈胜利 | Blanching culture method of box cultured chicory |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975832A (en) * | 2014-01-28 | 2014-08-13 | 华南农业大学 | Out-of-tube rooting method for tissue culture seedlings of silver birch |
| CN103975832B (en) * | 2014-01-28 | 2016-01-13 | 华南农业大学 | A kind of white wing silvery birch plantlet in vitro outside sprout-cultivating-bottle method |
| CN106258900A (en) * | 2016-08-26 | 2017-01-04 | 程依清 | A kind of method of Intelligent culture culture in glassware plant |
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| CN102369874B (en) | 2013-03-20 |
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