CN102369874B - Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes - Google Patents
Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes Download PDFInfo
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- CN102369874B CN102369874B CN201110249124XA CN201110249124A CN102369874B CN 102369874 B CN102369874 B CN 102369874B CN 201110249124X A CN201110249124X A CN 201110249124XA CN 201110249124 A CN201110249124 A CN 201110249124A CN 102369874 B CN102369874 B CN 102369874B
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Abstract
The invention discloses a method for carrying out differentiating, multiplication, rooting and stock exercising on tissue culture seedlings cultured outside test tubes, which belongs to a technique for stock growing. The method is characterized in that a culture vessel comprises a grid baseplate and a side plate, wherein the binding position of the grid baseplate and the side plate is higher than the lower edge of the side plate, the lower edge of the side plate is provided with a compact baseplate which is hermetically bound with the surrounding edges of the side plate, a part (below the binding position of the grid baseplate and the side plate) of the side plate is provided with an input pipe and an output pipe, an upper opening of a tray is movably combined with a stock-raising tray (for totally closed micro-environment control) with an upper cover, and the internal surface of the upper cover is provided with a spraying device and a lighting device. Compared with the prior art, the culture vessel is simple and practical, and has low manufacturing cost and use cost; the method is suitable to be used for carrying out effective control on micro environments in the differentiating, multiplication, rooting and stock exercising stages of various tissue culture seedlings cultured outside test tubes; a system for carrying out effective control on differentiating, multiplication, rooting and stock exercising micro-environments for tissue culture seedlings cultured outside test tubes can be formed by respectively connecting the input pipes and output pipes of multiple culture vessels and forming a pipe network, thereby realizing large-scale factory operating and production.
Description
Technical field: the invention belongs to the differentiation and proliferation hardening off method of taking root that the training seedling is organized in the plant regeneration field by tissue culture technique, particularly a kind of test tube outward.
Background technology: start at present " test-tube plantlet " heat both at home and abroad, many flowers, forest, fruit tree, vegetables all can be cultivated by tissue and carry out large-scale vegetative propagation.Owing to can carry out the cultivation of virus-free plant by the tissue cultivation, and all be under the environmental condition of artificial sterile working, to carry out large-scale artificial the cultivation and batch production production, therefore tissue culture technique has special role to the guaranteeing the quality of food resource, pure keeping and anti-season production, is expected to will form large industry in this century.Present various tissue cultivating and seedling technology, the differentiation and proliferation hardening of taking root of test tube being organized the training seedling outward all adopts the triangular glass bottle, adopt this culture vessel, it is large to exist the space that blake bottle occupies, transport difficulty is high, the transport space that its empty bottle and seedling bottle occupy when transportation is large, can cause simultaneously larger transportation loss; Have vial to be difficult to clean, the manpower that expends and expense have increased the production cost again; The differentiation and proliferation that test tube the is organized the training seedling outward culture environment such as the required temperature of hardening process, illumination of taking root rely on the equipment of being correlated with to realize in all must the parking space blake bottle outside in addition.
This shows that study and design a kind of culture vessel Simple And Practical, manufacturing cost and use cost is low, being fit to test tube that the scale batch production produces, to organize the differentiation and proliferation of the training seedling hardening off method of taking root outward be necessary.
Summary of the invention: the objective of the invention is to overcome the deficiencies in the prior art, study and design a kind of culture vessel Simple And Practical, manufacturing cost and use cost is low, is fit to test tube that the scale batch production produces and organizes the differentiation and proliferation of the training seedling hardening off method of taking root outward.
The present invention seeks to realize like this: the differentiation and proliferation that a kind of test tube the is organized the training seedling outward hardening off method of taking root, it is characterized in that the culture vessel employing comprises hollow out grid base plate, along the base plate edge and the side plate vertical with base plate, it is characterized in that the binding site of grid base plate and side plate is higher than the lower limb of side plate, the lower limb of side plate is designed with the sealing combination of its edge, closely knit base plate without the hollow out grid, side plate be positioned at grid base plate binding site under be designed with input pipe and efferent duct, the pallet seedling culturing tray for controlling full-sealed microenvironment that upper cover is arranged that agrees with actively suitable for reading.
Not on the same side, and the setting position of input pipe is higher than the setting position of efferent duct at the setting position on the side plate for described input pipe and efferent duct.All be designed with the check valve of control inputs or output on described input pipe and the efferent duct.The technique effect of above-mentioned design is; From be input into, efferent duct is carried moisture, nutriment and other promote root germinating, the material of growing, the ratio of transfer oxygen and carbon dioxide, temperature, humidity, more evenly rationally.The design of check valve is in case of necessity behind the valve-off, makes relative closure below the grid base plate of raising tray, organizes microenvironment below the training shoot root section outward to build better and to control the take root test tube of hardening phase of differentiation and proliferation.Described efferent duct is arranged at the side plate bottom near closely knit base plate.The technique effect of this design is that moisture that it(?) in case of necessity can be emptying that enter from input pipe or that infiltrate from culture vessel top, nutriment and other promote root germinating, the material of growing.
Suitable for reading the agreeing with actively of the end opening of described upper cover and pallet is existing by being designed with the concavo-convex tenon cause for gossip that interlocks.The inner face of described upper cover is designed with sprayer unit and illumination apparatus.The technique effect of above-mentioned design is: the end opening of the existing upper cover of the concavo-convex tenon cause for gossip that interlocks and pallet is suitable for reading agrees with actively, make on the one hand the take root test tube of hardening phase of the differentiation and proliferation on the top of raising tray organize training seedling microenvironment outward and realize preferably sealing, cooperate aforementioned closing in case of necessity on input pipe and the efferent duct behind the valve, make the microenvironment relative closure below the grid base plate of raising tray, so just formed the take root test tube of hardening phase of differentiation and proliferation and organized the from top to bottom better whole microenvironment of growing seedlings of sealing of training seedling outward.The design of sprayer unit and illumination apparatus is to realize the take root test tube of hardening phase of various different different cultivars differentiation and proliferations is organized effective control of the microenvironment key element that temperature, humidity and the illumination of training seedling requires outward; On the other hand, agree with actively that mean can be from the raising tray upper cover of removing suitable for reading, easily the take root test tube of hardening phase of the differentiation and proliferation in the raising tray is organized the training seedling outward and managed or transplant, also can be easily sprayer unit and illumination apparatus be safeguarded or repaired.
Technical scheme of the present invention is implemented through preliminary, has compared with prior art shown following beneficial effect:
1, culture vessel Simple And Practical, manufacturing cost and use cost is low.Compare with the triangular glass bottle that prior art adopts, only need to adopt the plastic process manufacturing, its transportation is compared large reduction with the consume in using with the glass blake bottle.
2, adapt to the various different cultivars differentiation and proliferations totally-enclosed microenvironment that the test tube of hardening phase organizes the training seedling outward of taking root is control effectively.A kind of seedling culturing tray for controlling full-sealed microenvironment that the invention process obtains at first through input pipe input steam, carries out disinfection to the take root medium of hardening of microenvironment and differentiation and proliferation; Secondly in the raising tray upper cover that has with sprayer unit and illumination apparatus of agreeing with actively suitable for reading, realize take root effective control of the microenvironment key element that temperature, humidity, the illumination of hardening phase requires of differentiation and proliferation.
3, as a kind of test tube is organized the system unit that training seedling growth microenvironment control effectively outward, only need will numerous culture vessels of the present invention input pipe and efferent duct connect into respectively pipe network, just can be combined into one test tube organized the seedling system that microenvironment control effectively of growing that trains outward.On this basis, carry out by stages and in groups take root hardening needs partition management of differentiation and proliferation according to test tube being organized the training seedling outward, just realize easily that test tube organizes the scale batch production of training seedling outward and manage and produce.
Description of drawings: Fig. 1 is the schematic diagram that meets culture vessel thematic structure of the present invention; Fig. 2 is the local enlarged diagram of the tenon mouth structure among Fig. 1; Fig. 3 is the structure perspective diagram of culture vessel of the present invention.
Embodiment: be described as follows below in conjunction with the implementation detail of accompanying drawing with culture vessel seedling culturing tray for controlling full-sealed microenvironment of the present invention:
Such as Fig. 1,2, shown in 3, a kind of test tube is organized the differentiation and proliferation hardening off method of taking root of training seedling outward, it is characterized in that the culture vessel employing comprises hollow out grid base plate 1, along the base plate edge and the side plate 2 vertical with base plate, it is characterized in that the binding site 3 of grid base plate and side plate is higher than the lower limb 21 of side plate, the lower limb of side plate is designed with the sealing combination of its edge, closely knit base plate 4 without the hollow out grid, the bottom that side plate is positioned at grid base plate binding site is designed with input pipe 5 and efferent duct 6, the pallet seedling culturing tray for controlling full-sealed microenvironment that upper cover 7 is arranged that agrees with actively suitable for reading.In conjunction with implementation, also shown among Fig. 1 and grown seedlings with matrix 11 and Seedling Stage nursery stock 12.
Shown in Fig. 1,3, not on the same side, and the setting position of input pipe is higher than the setting position of efferent duct at the setting position on the side plate for described input pipe 5 and efferent duct 6, and described efferent duct 6 is arranged at side plate 2 bottoms near closely knit base plate 4.All be designed with the check valve 51,61 of control inputs or output on described input pipe 5 and the efferent duct 6.
As shown in Figure 1, 2, suitable for reading the agreeing with of the end opening of described upper cover 7 and pallet is that the concavo-convex tenon mouth that interlocks 8 realizes by being designed with.The inner face of described upper cover 7 is designed with sprayer unit 9.The inner face of described upper cover also is designed with illumination apparatus 10.
The test tube of embodiment floral leaf sweet-scented oleander is organized the differentiation and proliferation hardening off method of taking root of training seedling outward
[0018] step 1: the bud seedling that will from test tube, grow up to, shear, transfer in breed cultivation in the seedling culturing tray for controlling full-sealed microenvironment that contains proliferated culture medium of sterilization, described proliferated culture medium is: every liter of minimal medium adds: zeatin (ZT) 0.5-1.5 milligram, 6 one benzyl purine 1.0-2.0 milligrams, indolebutyric acid 0.05-0.2 milligram and active carbon (Charcoal) 1000-2000 milligram; Step 2: with the bud seedling of cultivating in the step 1, shear, be inoculated in the seedling culturing tray for controlling full-sealed microenvironment that contains the strong seedling culture base of sterilization, carry out strong seedling culture, enter next step after 30 days, described strong seedling culture base is: every liter of minimal medium adds: zeatin (ZT) 0.2-1.0 milligram, 6-benzyl purine 0.5-1.0 Bo gram, indolebutyric acid 0.1-0.3 milligram and active carbon (Charcoal) 1000-2000 milligram; Step 3: with the strong sprout of cultivating in the step 2, remove base portion callus and partial blade, stay 4-5 sheet blade, be inoculated in containing of sterilization taken root the former basic seedling culturing tray for controlling full-sealed microenvironment of processing generation root, carried out culture of rootage 7-12 days, it is that every liter of minimal medium adds that the described processing of taking root produces the former basic medium of root: 0c-methyl α-naphthyl acetate (NAA) 0.2-0.5 milligram, indolebutyric acid 0.4-0.8 milligram and active carbon (Charcoal) 2000-3000 milligram; Step 4: with the unrooted seedling with the former base of root of growth in the step 3, take out and clean, be transplanted to and contain peat: in the matrix of the seedling culturing tray for controlling full-sealed microenvironment of yellow soil=1: 1 (volume ratio), water permeable, keep 25-28 ℃ of temperature, relative moisture is more than 85%, shading (front 10 days) 75%, after see gradually light, taking root after 25 days survives; Step 5: growth in the step 4 had with the upper cover of the seedling culturing tray for controlling full-sealed microenvironment of root seedling remove, allow band root seedling under natural environment, grow and carried out hardening in 15 days, then transplant to cultivation field.
Claims (6)
1. the test tube differentiation and proliferation of the organizing the training seedling outward hardening off method of taking root, it is characterized in that the culture vessel employing comprises hollow out grid base plate (1), along the base plate edge and the side plate (2) vertical with base plate, the binding site of grid base plate and side plate (3) is higher than the lower limb (21) of side plate, the lower limb of side plate is designed with the sealing combination of its edge, closely knit base plate (4) without the hollow out grid, the bottom that side plate is positioned at grid base plate binding site is designed with input pipe (5) and efferent duct (6), the pallet seedling culturing tray for controlling full-sealed microenvironment that upper cover (7) are arranged that agrees with actively suitable for reading.
2. a kind of test tube according to claim 1 is organized the differentiation and proliferation hardening off method of taking root of training seedling outward, it is characterized in that described input pipe (5) and efferent duct (6) at the setting position on the side plate not on the same side, and the setting position of input pipe is higher than the setting position of efferent duct, and described efferent duct (6) is arranged at side plate (2) bottom near closely knit base plate (4).
3. a kind of test tube according to claim 1 and 2 is organized the differentiation and proliferation hardening off method of taking root of training seedling outward, it is characterized in that all being designed with on described input pipe (5) and the efferent duct (6) check valve (51), (61) of control inputs or output.
4. a kind of test tube according to claim 1 is organized the differentiation and proliferation hardening off method of taking root of training seedling outward, it is characterized in that the end opening of described upper cover (7) and suitable for reading the agreeing with of pallet are by being designed with concavo-convex tenon mouth (8) realization that interlocks.
5. a kind of test tube according to claim 1 is organized the differentiation and proliferation hardening off method of taking root of training seedling outward, it is characterized in that the inner face of described upper cover (7) is designed with sprayer unit (9).
6. a kind of test tube according to claim 1 is organized the differentiation and proliferation hardening off method of taking root of training seedling outward, it is characterized in that the inner face of described upper cover (7) is designed with illumination apparatus (10).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110249124XA CN102369874B (en) | 2011-08-29 | 2011-08-29 | Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes |
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| CN201110249124XA CN102369874B (en) | 2011-08-29 | 2011-08-29 | Method for carrying out differentiating, multiplication, rooting and acclimatization on tissue culture seedlings cultured outside test tubes |
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| CN102369874A CN102369874A (en) | 2012-03-14 |
| CN102369874B true CN102369874B (en) | 2013-03-20 |
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| CN103975832B (en) * | 2014-01-28 | 2016-01-13 | 华南农业大学 | A kind of white wing silvery birch plantlet in vitro outside sprout-cultivating-bottle method |
| CN106258900B (en) * | 2016-08-26 | 2019-02-12 | 程依清 | A kind of method of Intelligent culture culture in glassware plant |
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| KR100859599B1 (en) * | 2007-11-02 | 2008-09-23 | 주식회사 한테크 | Vinyl house providing a temperature control and ventilating |
| JP2009119357A (en) * | 2007-11-14 | 2009-06-04 | Fulta Electric Machinery Co Ltd | Centrifugal atomization humidifier apparatus |
| CN201345844Y (en) * | 2008-11-24 | 2009-11-18 | 孟树新 | Spraying plant-cultivating device |
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN101982061B (en) * | 2010-08-30 | 2012-03-14 | 陈胜利 | Blanching culture method of box cultured chicory |
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