CN102366383A - Marine organism functional cosmetic for nourishing skin around eyes - Google Patents
Marine organism functional cosmetic for nourishing skin around eyes Download PDFInfo
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- CN102366383A CN102366383A CN2011102887162A CN201110288716A CN102366383A CN 102366383 A CN102366383 A CN 102366383A CN 2011102887162 A CN2011102887162 A CN 2011102887162A CN 201110288716 A CN201110288716 A CN 201110288716A CN 102366383 A CN102366383 A CN 102366383A
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Abstract
The invention provides a marine organism functional cosmetic for nourishing skin around the eyes. The marine organism functional cosmetic comprises, on the basis of the total mass of the cosmetic, the following active components by mass: 0.1 to 10% of marine mollusk active peptide, 0.1 to 10% of marine animal collagen peptide, 0.05 to 5% of algal polysaccharide, 0.01 to 2% of chondroitin sulfate and 0.2 to 15% of pearl powder; the cosmetic can further comprises the following active components by mass: 0.05 to 0.6% of allantoin, 0.01 to 1% of coenzyme Q10, 0.1 to 5% of VC phosphate magnesium and 0.1 to 5% of VE acetate. According to the invention, pure natural marine organism extracts are used as main raw materials, and the extracts can absorbed by skin rapidly, are safe and mild, cause no irritation on skin and bring no burden to skin; stabilized synergism of marine biologically-active substances and other active substances is utilized in the invention, which enables a nourishing effect of the cosmetic on skin around the eyes to be substantially enhanced.
Description
Technical field
The present invention is specifically related to a kind of marine organisms function cosmetics, is specifically related to a kind of marine organisms function cosmetics of skin near the eyes that are used to nurse.
Background technology
Skin is the most tender and lovely facial weakness, fatiguability and old and feeble position near the eyes.Its structure is comparatively loose, slides easily and forms fold, and its thickness is merely 1/3 of facial skin, and sebaceous gland and sweat gland distribute minimum, and moisture is prone to evaporation and the moisture-retaining capacity congenital defect.Because ocular nerve fiber and blood capillary distribution density are higher, and blood circulation is slower with the lymphsystem operation, very easily forms congestion near the eyes and seems eye fatigue or dull.Its fat content is lower, and skin corium lack flexibility fiber and collagen structure add to make it continue tension up to ten thousand times nictation of every day or loosen that skin also very easily follows the string and becomes lax near the eyes, thereby exposes people's the true age.Along with the quickening of modern society's life, work rhythm and popularizing of network; Add that daily life system is irregular, stress is big, influence of various factors such as in bad health, improper, old and feeble, Exposure to Sunlight and radiation with eye; Problems such as black eye, eyeprint, eye pouch, edema appear in skin regular meeting near the eyes, let the people seem more wan and sallow, tired and old and feeble.
At present, the cosmetics of nursing skin near the eyes on the market are less, and mostly its effective ingredient is Azelaic Acid, Chinese herbal medicine extract, hormone etc., and not only zest is big, effect is not obvious, and long-time use can make skin produce to rely on.These cosmetics focus mostly in wrinkle removal, Vitamin K, to smoothing eye pouch, alleviating the eye edema and have little effect.Therefore; Concerning by the personage that likes to be beautiful of various problems such as black eye, wrinkle, eye pouch, edema puzzlement; A kind of skin care cosmetics near the eyes can't satisfy the demands at all; And the cosmetics that use several kinds of different efficacies simultaneously not only expensive, complex operation, and can make near the eyes that skin too much untoward reaction such as fat granule, pruritus, decortication, redness occur because of cosmetics use, skin problem becomes even more serious thereby make near the eyes.Therefore, be badly in need of developing a kind of safer, gentle, lastingly and comprehensive effects skin care cosmetics near the eyes.
The marine bioactivity extract relies on himself good biocompatibility; Conclusive moistening water conservation effect and resist ultraviolet injury significantly, suppress melanin and generate, promote effects such as collagen protein synthesis has received scientific research personnel's the attention and the favor on beauty treatment circle.Though marine bioactivity materials such as collagen peptide, Sargassum polysaccharides, Margarita powder, oligonucleotide, chondroitin sulfate, carotenoid have been developed to all kinds of cosmetics, be used for total care near the eyes the marine organisms function cosmetics of skin still belong to blank.In addition; Seashells as one of most precious living marine resources; Its bioactive peptide also has effects such as significant promotion wound healing, promotion collagen protein synthesis; Though at present existing patent discloses the seashells anti-oxidation peptide and can be used for cosmetics, do not relate to actual technique for applying technology and prescription, it is more still not mentioned in the application of nursing skin function cosmetics near the eyes.
Summary of the invention
The objective of the invention is to the existing skin class cosmetic industry of nursing near the eyes very littlely or zest big, life-time service skin is prone to produce marine bioactivity materials such as dependence, too refinement of effect, seashells bioactive peptide and problem such as in nursing near the eyes the skin function cosmetics, does not use as yet, provide that a kind of safer, gentle, effect is persistent to be used to nurse the marine organisms function cosmetics of skin near the eyes.
Above-mentioned purpose of the present invention realizes through following technical scheme: a kind ofly be used to nurse the marine organisms function cosmetics of skin near the eyes, in marine organisms function cosmetics gross mass, contain the active component of following quality percentage composition:
Seashells bioactive peptide 0.1-10%
Marine animal collagen peptide 0.1-10%
Sargassum polysaccharides 0.05-5%
Chondroitin sulfate 0.01-2%
Margarita powder 0.2-15%.
Above-mentionedly be used to nurse the marine organisms function cosmetics of skin near the eyes, in marine organisms function cosmetics gross mass, the quality percentage composition of each active component is preferably:
Seashells bioactive peptide 0.5-6%
Marine animal collagen peptide 0.5-6%
Sargassum polysaccharides 0.1-1%
Chondroitin sulfate 0.05-1%
Margarita powder 0.5-5%.
For strengthening Vitamin K, dispel eyeprint, smooth eye pouch, eliminate effect such as edema, the active component of marine organisms function cosmetics of the present invention can also comprise allantoin, ubiquinone
10, V
CMagnesium phosphorate and V
EAcetate, its quality percentage composition in marine organisms function cosmetics is preferably allantoin 0.05%-0.6%, ubiquinone
100.01%-1%, V
CMagnesium phosphorate 0.1%-5%, V
EAcetate 0.1%-5%.
In above-mentioned active component, described seashells bioactive peptide can prepare through the method that contains following operation: with the seashells bioactive peptide that makes after ocean shellfish meat process protease hydrolyzed, the ultrafiltration of 3000Da ultrafilter membrane; Said seashells preferably includes one or more in Paphia Undulata, Corbiculida, Margarita, scallop, mussel and the Concha Ostreae, and wherein the best is a Paphia Undulata; Said protease comprises one or more in bromelain, papain, neutral protease, alkaline protease, pepsin, trypsin and the subtilisin.
Wherein specifically the seashells bioactive peptide can prepare through following method: get the edible seashells that meets China's relevant marine product use standard, add 2-4 times of volume pure water homogenate 3-5min behind the internal organs that shell, room temperature adds protease; Enzyme concentration is a 1000-3000U/g shellfish meat; At pH is 6.5-7.5, and temperature is 40-60 ℃, enzymolysis 30-90min; Boiling water bath enzyme denaturing 5-8min; The centrifugal 10-15min of 5000-8000r/m, supernatant is the ultrafiltration of 3000Da ultrafilter membrane through molecular cut off, lyophilization makes the seashells active peptide powder.
More than be the preferred method of extracting the seashells bioactive peptide, in the practical application in industry, adopt commercially available seashells bioactive peptide also to be fine.
Marine animal collagen peptide of the present invention comprises one or more in cod collagen peptide, sharkskin collagen peptide, holothurian collagen peptide and the collagen from squid skin peptide; It can prepare according to methods such as existing disclosed protease hydrolyzeds, and is also commercially available.
Sargassum polysaccharides of the present invention comprises one or more in sodium alginate, Porphyra Polysaccharide, Sargassum polysaccharide, Thallus Laminariae polysaccharide, sargassan, Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) polysaccharide, Eucheuma gelatinosum polysaccharide, Eucheuma striatum polysaccharide and the Hijiki polysaccharide; It is commercially available, also can be according to existing method preparation.
Chondroitin sulfate of the present invention is the extract of marine animal cartilage, and wherein said marine animal is one or more in shark, Acipenser Sinensis,
fish,
fish, tilapia, Stichopus japonicus and the squid; Described chondroitin sulfate is commercially available, also can be according to existing method preparation.
Margarita powder of the present invention comprises one or more in Margarita ultrafine powder, water soluble Margarita ultrafine powder, instant Margarita powder and the pearl hydrolysate liposome; Above-mentioned Margarita powder is commercially available, also can be according to existing method preparation.
In above-mentioned active component, allantoin, ubiquinone
10, V
CMagnesium phosphorate, V
EAcetate is commercially available.
Above-mentioned active component is carried out homogenizing through common process with conventional cosmetic base (commercially available) component of this area or mixes; Promptly can be made into cream frost, emulsion, gel, gel or water preparation, the cosmetics good stability among the present invention, coating are evenly, spreadability is good.
Little, the good water solubility of seashells bioactive peptide molecular weight in the marine organisms function cosmetics of the present invention; Can infiltrate skin surface rapidly and keep cell moisture, promotion lymphsystem and blood microcirculation, promotion cell metabolism and collagen protein synthesis, removing free radical, the generation of inhibition melanin; Can effectively keep near the eyes the Skin Cell vigor, skin become compact and high resilience, to improve pigment type and vascular type black eye, lusitropic eye pouch, eye edema, dried stricture of vagina and microgroove effect are obvious near the eyes.
Marine animal collagen protein Toplink in the marine organisms function cosmetics of the present invention promotes fibroblast collagen and hyaluronic generation; Can repair the damaged skin tissue, keep epidermis moistening, improve skin glossy, reappear youthful vigor, very obvious to improving effects such as skin elasticity, the dried stricture of vagina of eye, microgroove, crows-feet and expression stricture of vagina.
Sargassum polysaccharides in the marine organisms function cosmetics of the present invention can form thin film and moisture is permeated in the horny layer to keep moisture of skin near the eyes at skin surface; It can also promote horn cell and SF growth, remove free radical; Have good moisture preserving, repair effect, to the black eye that cause because of drying, Exposure to Sunlight, radiation, eye pouch to improve effect obvious.
Chondroitin sulfate in the marine organisms function cosmetics of the present invention is a kind of acid mucopolysaccharide, also is one of composition important in the ocular tissue; It can effectively promote fiber growth in the substrate, strengthens cutaneous permeability, make lax skin near the eyes recover elasticity; Can effectively prevent moisture of skin evaporation near the eyes after forming gel with water, to alleviate eye tired, improve that lax skin, dried stricture of vagina, microgroove, crows-feet and expression stricture of vagina effect are obvious near the eyes.
Margarita powder in the marine organisms function cosmetics of the present invention contains the glycine of needed by human etc. and promotes trace element such as regenerated aminoacid of skin collagen and Zn; Have effects such as toxin expelling, the small wound healing of acceleration; Can effectively promote reepithelialization, skin whitening; To improve pigment type black eye, eye mottle effect is very obvious, makes skin delicate, the brilliant white that becomes.
Allantoin in the marine organisms function cosmetics of the present invention can promote epithelial cell growth, influence the quick spheroidizing of epidermis; When peeling off the metabolism cutin, can also give intercellular substance competent moisture; Make coarse skin become smooth and plump, obvious to improving the dried stricture of vagina of eye, microgroove, crows-feet and expression stricture of vagina effect.
Ubiquinone in the marine organisms function cosmetics of the present invention
10It is endogenous ubiquinone in the epidermis cell
10Effectively replenish; Can promote skin metabolism and epidermal cell proliferation, accelerate blood microcirculation, reduce pigmentation and collagen degradation, the recovery skin elasticity, to because of the black eye that wear out, malnutrition, illumination, disease etc. cause, eye edema, eyeprint to improve effect obvious.
V in the marine organisms function cosmetics of the present invention
CThe magnesium phosphorate chemical property is stable, infiltrates behind the skin by the phosphate enzymolysis V that can dissociate
CV
CCan effectively remove free radical, desalination and minimizing pigmentation, promote the structure organization collagen protein to generate, repair and the old and feeble damaged skin of regenerating, obvious to improving pigment type black eye, eyeprint and lusitropic eye pouch effect.
V in the marine organisms function cosmetics of the present invention
EAcetate character in Aquo System is more stable, is discharged free V by the lipase enzymolysis behind the infiltration skin
EV
EBe polyphenoils and membrane stabilizer important in the cell membrane; Can pin moisture of skin, remove free radical, suppress lipid peroxidation, keep between elastic fiber and collagen tissue contact, quicken cytothesis and regeneration, to improve crows-feet, the eye pouch effect is very obvious.
Marine organisms function cosmetics among the present invention nurse that skin effects is distinguished near the eyes, wherein marine bioactivity material and allantoin, ubiquinone
10, V
CMagnesium phosphorate and V
EAcetate has the collaborative complex role of good forward, and effects such as xerosis cutis, pigmentation, cutis laxa, eyeprint significantly strengthen to make it improve near the eyes.
Solution of the present invention be based on the inventor to black eye, eyeprint, eye pouch and edema etc. near the eyes skin problem various influence factors and form mechanism; And to the understanding of the beauty and skin care effect of marine bioactivity material; In conjunction with modern cosmetics and pharmacological research achievement; From numerous marine bioactivity extracts, optimize to have and nurse the raw material of skin effect near the eyes, and carry out scientific composition, make it give full play to the effect of skin near the eyes of nursing with collaborative composite.
Need to prove; Because eye part skin is the position of facial the most tender and lovely weakness; The cosmetics that generally can be used for skin nursing near the eyes; Its skin care item that can be used as other body part skin equally use, and promptly the marine organisms function cosmetics among the present invention not only can be used for nursing skin near the eyes, also can be used for the nursing at other position of health equally.That is to say that cosmetics of the present invention can be used as ordinary cosmetics or have the function cosmetics of nursing skin effect near the eyes.
Compared with prior art, the present invention has following beneficial effect:
(1) to the special physiology's structure of skin near the eyes and to the sensitivity of the used composition of cosmetics; It is primary raw material that marine organisms function cosmetics among the present invention have been selected the marine organism extract of pure natural for use; Its marine bioactivity material can be rapidly by skin absorbs; Safe, gentle, do not cause skin allergy, can not bring any burden to skin yet;
(2) marine organisms function cosmetics of the present invention from replenishing water and preserving moisture, promote that collagen protein and elastic fiber are synthetic, promote lymphsystem and blood vessel microcirculation, promote skin cell metabolism, inhibition and eliminate pigmentation, resist ultraviolet and many-sides such as electromagnetic radiation, removing free radical play a role; Effectively alleviate and improve comprehensively pigment type and vascular type black eye, eye edema, or eye pouch that fatigue form lax, multiple skin problems near the eyes such as dried stricture of vagina, microgroove, crows-feet and expression stricture of vagina near the eyes because of eye; Skin is become more compact, high resilience, let radiant, the perpetual youth of people;
(3) the present invention utilizes the stabilisation synergism between marine bioactivity material and other active substance, and skin effects has obtained remarkable enhancing to make marine organisms function cosmetics among the present invention nurse near the eyes;
(4) the marine organisms function cosmetics among the present invention are numerous in variety; Be applicable to the crowd of different sexes, age and skin matter; Be particularly useful for that black eye are arranged, the personage that likes to be beautiful of skin problem puzzlement near the eyes such as eyeprint, eye pouch, edema, can be used as and nurse the common skin care item or the function cosmetics of skin near the eyes; Equally also can be used as the nursing materials of other body part.
Description of drawings
Fig. 1 a is to the influence of Mice Auricle capillary vessel number behind tail vein injection sample 1 among the embodiment 11, sample 2 and the blank;
Fig. 1 b is the influence of the a-f group back of tail vein injection sample 1 among the embodiment 11 to the Mice Auricle capillary vessel number;
Fig. 1 c is the influence of the A-F group back of tail vein injection sample 2 among the embodiment 11 to the Mice Auricle capillary vessel number;
Fig. 1 d is the A series A of tail vein injection sample 2 among the embodiment 11
1-A
6The group back is to the influence of Mice Auricle capillary vessel number;
Fig. 2 a is to the influence of Mice Auricle thin vein caliber behind tail vein injection sample 1 among the embodiment 11, sample 2 and the blank;
Fig. 2 b is the influence of the a-f group back of tail vein injection sample 1 among the embodiment 11 to Mice Auricle thin vein caliber;
Fig. 2 c is the influence of the A-F group back of tail vein injection sample 2 among the embodiment 11 to Mice Auricle thin vein caliber;
Fig. 2 d is the A of A series in the tail vein injection sample 2 among the embodiment 11
1-A
6The group back is to the influence of Mice Auricle thin vein caliber;
Fig. 3 a is to the influence of Mice Auricle arteriole caliber behind tail vein injection sample 1 among the embodiment 11, sample 2 and the blank;
Fig. 3 b is the influence of the a-f group back of tail vein injection sample 1 among the embodiment 11 to Mice Auricle arteriole caliber;
Fig. 3 c is the influence of the A-F group back of tail vein injection sample 2 among the embodiment 11 to Mice Auricle arteriole caliber;
Fig. 3 d is A in the A series of tail vein injection sample 2 among the embodiment 11
1-A
6The group back is to the influence of Mice Auricle arteriole caliber;
Fig. 4 a is to the influence of Mice Auricle arteriole flow velocity behind tail vein injection sample 1 among the embodiment 11, sample 2 and the blank;
Fig. 4 b is that the a-f in the tail vein injection sample 1 organizes the influence of back to Mice Auricle arteriole flow velocity among the embodiment 11;
Fig. 4 c is that the A-F in the tail vein injection sample 2 organizes the influence of back to Mice Auricle arteriole flow velocity among the embodiment 11;
Fig. 4 d is the A in the A series in the tail vein injection sample 2 among the embodiment 11
1-A
6The group back is to the influence of Mice Auricle arteriole flow velocity;
Fig. 5 a is cosmetic base among the embodiment 12, do not contain the marine bioactivity composition, only contain 6 pairs in marine bioactivity composition and sample 1-sample near the eyes the keratodermatitis water content improve design sketch;
Fig. 5 b be among the embodiment 12 in the sample 6 a-f group to the design sketch that improves of keratodermatitis water content near the eyes;
Fig. 6 a is cosmetic base among the embodiment 12, do not contain the marine bioactivity composition, only contain 6 pairs in marine bioactivity composition and sample 1-sample near the eyes skin color improve design sketch;
Fig. 6 b be among the embodiment 12 in the sample 6 a-f group to the design sketch that improves of skin color near the eyes;
Fig. 7 a is cosmetic base among the embodiment 12, do not contain the marine bioactivity composition, only contain 6 pairs in marine bioactivity composition and sample 1-sample near the eyes wrinkle of skin improve design sketch;
Fig. 7 b be among the embodiment 12 in the sample 6 a-f group to the design sketch that improves of wrinkle of skin near the eyes;
Fig. 8 a is cosmetic base among the embodiment 12, do not contain the marine bioactivity composition, only contain 6 pairs in marine bioactivity composition and sample 1-sample near the eyes skin elasticity improve design sketch;
Fig. 8 b be among the embodiment 12 in the sample 6 a-f group to the design sketch that improves of skin elasticity near the eyes.
The specific embodiment
Below in conjunction with accompanying drawing the present invention is described further, but embodiment of the present invention is not limited to following embodiment introduction all variation that is equal to or flexible categories of doing according to method of the present invention that all should be regarded as the present invention's protection.Below raw materials used, except that special indicating, be commercially available.
Get the edible Paphia Undulata that meets China's relevant marine product use standard, add 2 times of volume pure water homogenate 4min behind the internal organs that shell, boil 5min; Be cooled to and add the powdery papain after the room temperature, enzyme concentration is a 1000U/g shellfish meat, under the room temperature behind the 170w ultrasonic Treatment 8min; PH is 7, and temperature is 50 ℃, enzymolysis 60min; Boiling water bath enzyme denaturing 6min; The centrifugal 15min of 8000r/m, supernatant is the ultrafiltration of 3000Da ultrafilter membrane through molecular cut off, makes the Paphia Undulata active peptide powder after the lyophilization.
The A phase: carbomer 0.2%, glycerol 4%, propylene glycol 3%, deionized water 42.6% are the quality percentage composition.
The B phase: ceteareth-2 1.5%, ceteareth-21 2%, Jojoba oil 3%, cetearyl alcohol 2%, glyceryl monostearate 1%, caprylic/capric triglyceride 4%, ethylhexyl palmitate 3%, polydimethylsiloxane 2%, isopropyl myristate 3%, ethyl hydroxybenzoate 0.1% are the quality percentage composition.
C phase: get Paphia Undulata bioactive peptide 6%; Be selected from the cod collagen peptide 3% that the little ecological Science and Technology Ltd. of Cacumen et folium clerodendri mandarinori (Clerodendron mandarinorum Diels) difficult to understand buys from Qingdao; Be selected from the sodium alginate 1% that profit chemical industry company limited is bought from the sea, Yantai City; Be selected from the shark chondroitine 0.2% that sunrise Industrial Co., Ltd. buys from the Qufu City sea; Be selected from the instant Margarita powder of buying from Huzhou Zhenlu Biological Products Co., Ltd. 15%; Be selected from the allantoin 0.1% that male chemical industry company limited is bought from east, Guangzhou; Be selected to pray and rise the V that trade Co., Ltd buys from Guangzhou
CMagnesium phosphorate 0.5%, be selected from the ubiquinone that from hero Warner biological engineering company limited is bought
100.5%, is selected from the V that achievement commerce and trade company limited is bought from Zhengzhou, Henan Province
EAcetate 1% is the quality percentage composition.
The D phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.2%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: heat respectively A mutually with B to 80 ℃, stir time with B be added to A mutually in.Stirring is cooled to 45 ℃, C is added in the mixture of A, B phase, mix 10min after, add the D phase, mix homogenizing and make and be used to nurse the marine organisms function cosmetics cream frost of skin near the eyes.
Get the edible Corbiculida and shell and add 2.5 times of volume pure water homogenate 5min behind the internal organs, boil 10min, add powdered bromelain and subtilisin (mass ratio is 1: 1) after being cooled to room temperature; Enzyme concentration is a 2000U/g shellfish meat, is 6.5 at pH, and temperature is 40 ℃; Enzymolysis 30min, boiling water bath enzyme denaturing 7min, the centrifugal 12min of 6000r/m; Supernatant is the ultrafiltration of 3000Da ultrafilter membrane through molecular cut off, makes the Corbiculida active peptide powder after the lyophilization.
The A phase: carbomer 0.1%, glycerol 5%, propylene glycol 3%, deionized water 53.8% are the quality percentage composition.
The B phase: ceteareth-2 1.2%, ceteareth-21 1.5%, Parleam 3%, caprylic/capric triglyceride 3%, ethylhexyl palmitate 3%, polydimethylsiloxane 2%, ethyl hydroxybenzoate 0.1% are the quality percentage composition.
C phase: the Corbiculida peptide 2 % that gets above-mentioned preparation; Be selected from the sharkskin collagen peptide 6% that following bio tech ltd is bought from Qingdao; Be selected from the Eucheuma striatum polysaccharide 0.2% that emerging standing grain chemical products company limited is bought from Zhengzhou; Be selected from the Hijiki polysaccharide 0.3% that three commerce and trade company limiteies are bought from Zhengzhou; Be selected from the Acipenser Sinensis chondroitin sulfate 1% that moistens precious bio tech ltd purchase from the Longyan; Be selected from the water soluble Margarita ultrafine powder 5% that Hemerocallis citrina Baroni Pharmaceutical Technology Co., Ltd buys from Zhuji, Zhejiang; Allantoin 0.6%; V
CMagnesium phosphorate 2%, ubiquinone
101%, V
EAcetate 5% is the quality percentage composition.
The D phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.1%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: heat respectively A mutually with B to 80 ℃, stir time slowly with B be added to A mutually in.Stirring is cooled to 45 ℃, C is added in the mixture of A, B phase, mix 10min after, add the D phase, mix homogenizing and make and be used to nurse the marine organisms function cosmetic emulsion of skin near the eyes.
Add 3 times of volume pure water homogenate 3min after getting edible Margarita, scallop (mass ratio is 1: the 1) internal organs that shell, add powdered neutral protease and pepsin (mass ratio is 1: 1), enzyme concentration is a 3000U/g shellfish meat; At pH is 7.5, and temperature is 60 ℃, enzymolysis 60min; Boiling water bath enzyme denaturing 8min; The centrifugal 10min of 7000r/m, supernatant are that the ultrafilter membrane of 3000Da carries out ultrafiltration through molecular cut off, make Margarita and scallop active peptide powder after the lyophilization.
A phase: HANSHENGJIAO 0.4%, acrylic ester/C
10-30Alkyl acrylate cross-linked polymer 0.6%, EDTA-2Na0.1%, glycerol 5%, propylene glycol 5%, butanediol 5%, deionized water 38.5% are the quality percentage composition.
B phase: get active pearl shell peptide 0.2% that method for preparing obtains, scallop active peptide 0.3% that method for preparing obtains, commercially available collagen from squid skin peptide 10%, navigator's chemical products company limited is bought from Henan sargassan 1%, navigator's chemical products company limited is bought from Henan Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) polysaccharide 2%, navigator's chemical products company limited is bought from Henan Eucheuma gelatinosum polysaccharide 2%, commercially available
Fish chondroitin sulfate 1%, according to the tilapia chondroitin sulfate 1% of people's such as growth jade " the response surface method is optimized the research of leftovers of tilapia process for extracting chondroitin sulfate " method preparation, be selected from the Margarita ultrafine powder 0.2% bought from the refined article of everyday use company limited of the happy U.S. of Zhuji City, be selected from the instant Margarita powder 0.3%, allantoin 0.3%, the V that buy from Huzhou Zhenlu Biological Products Co., Ltd.
C Magnesium phosphorate 5%, ubiquinone
100.2%, V
EAcetate 0.5%, deionized water 20% are the quality percentage composition.
The C phase: PEG-40 hydrogenated castor grease 1%, triethanolamine 0.2%, essence 0.1%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: mix the A phase component and be heated to 80 ℃ stirring, adding is pre-mixed uniform B phase when being cooled to 45 ℃, stirs 10min to transparent, makes behind the adding C phase stirring and evenly mixing to be used to nurse the marine organisms function cosmetic gel of skin near the eyes.
Get and meet the edible mussel that the relevant marine product of China uses standard, add 4 times of volume pure water homogenate 4min behind the internal organs that shell, add powdered trypsin and alkaline protease (mass ratio is 1: 2) after being cooled to room temperature; Enzyme concentration is a 3000U/g shellfish meat; PH is 7, temperature is 50 ℃ of enzymolysis 90min, boiling water bath enzyme denaturing 5min, the centrifugal 15min of 5000r/m; Supernatant is the ultrafiltration of 3000Da ultrafilter membrane through molecular cut off, makes mussel active peptide powder after the lyophilization.
A phase: HANSHENGJIAO 0.2%, acrylic ester/C
10-30Alkyl acrylate cross-linked polymer 0.6%, glycerol 5%, propylene glycol 3%, deionized water 45% are the quality percentage composition.
B phase: mussel active peptide 10%; Holothurian collagen peptide 0.5%; The Porphyra Polysaccharide 0.1% that three commerce and trade company limiteies are bought from Zhengzhou; Stichopus japonicus chondroitin sulfate 0.02% according to people's such as Wu Xiaolin " preparation of heterogeneity marine sulfate polysaccharide and improve the comparative study of rat fat liver effect " method preparation; Squid chondroitin sulfate 0.03% according to the preparation of people's such as Chen Xiaoe " Study on extraction of chondroitin sulfate in the squid cartilage " method; Margarita ultrafine powder 0.4%; Water soluble Margarita ultrafine powder 0.6%; Allantoin 0.05%; V
CMagnesium phosphorate 1%, ubiquinone
100.1%, V
EAcetate 2%, deionized water 30% are the quality percentage composition.
The C phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.3%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: mix the A phase component and be heated to 80 ℃ stirring; Add when being cooled to 45 ℃ and be pre-mixed uniform B phase; Stir homogenizing 3min, be sequentially added into C each component mutually under the room temperature, stirring to make is used to nurse the marine organisms function cosmetics gel of skin near the eyes.
According to the method for preparing the seashells bioactive peptide in the embodiment 1, enzymolysis makes oyster active peptides powder, active pearl shell peptide powder.
The A phase: glycerol 5%, propylene glycol 3%, butanediol 2%, deionized water 50% are the quality percentage composition.
The B phase: oyster active peptides 0.05%, active pearl shell peptide 0.05%, cod collagen peptide 0.02%, collagen from squid skin peptide 0.08%, according to the Sargassum polysaccharide 0.02% of people's such as Zhao Yu " Study on Extraction Method of Sargassum polysaccharide " method preparation, according to the Thallus Laminariae polysaccharide 0.03% of the method preparation of people such as Men Xiaoyuan " preparation of Thallus Laminariae sulfated polysaccharide and property research thereof ", according to Zhang Xiaojun etc. "
The extraction of fish chondroitin sulfate and property research " method preparation
Fish chondroitin sulfate 0.01%, Dao Feng trade Co., Ltd buys from Guangzhou pearl hydrolysate liposome 0.2%, allantoin 0.2%, V
CMagnesium phosphorate 0.1%, ubiquinone
100.01%, V
EAcetate 0.1%, deionized water 37.83% are the quality percentage composition.
The C phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.2%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: mix earlier the A phase, more in advance the B of mixing group add A mutually in, mix the back and add the C phase component, mixing makes and is used to nurse the marine organisms function cosmetics water preparation of skin near the eyes.
According to the method for preparing the seashells bioactive peptide in the embodiment 1, enzymolysis makes the Paphia Undulata active peptide powder.
The A phase: carbomer 0.2%, glycerol 4%, propylene glycol 3%, deionized water 44.7% are the quality percentage composition.
The B phase: ceteareth-2 1.5%, ceteareth-21 2%, Jojoba oil 3%, cetearyl alcohol 2%, glyceryl monostearate 1%, caprylic/capric triglyceride 4%, ethylhexyl palmitate 3%, polydimethylsiloxane 2%, isopropyl myristate 3%, ethyl hydroxybenzoate 0.1% are the quality percentage composition.
The C phase: Paphia Undulata bioactive peptide 6%, cod collagen peptide 3%, sodium alginate 1%, shark chondroitine 0.2%, instant Margarita powder 15% are the quality percentage composition.
The D phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.2%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: heat respectively A mutually with B to 80 ℃, stir time with B be added to A mutually in.Stirring is cooled to 45 ℃, C is added in the mixture of A, B phase, mix 10min after, add the D phase, mix homogenizing and make and be used to nurse the marine organisms function cosmetics cream frost of skin near the eyes.
Embodiment 7
According to the method for preparing the seashells bioactive peptide in the embodiment 2, enzymolysis makes the Corbiculida active peptide powder.
The A phase: carbomer 0.1%, glycerol 5%, propylene glycol 3%, deionized water 62.4% are the quality percentage composition.
The B phase: ceteareth-2 1.2%, ceteareth-21 1.5%, Parleam 3%, caprylic/capric triglyceride 3%, ethylhexyl palmitate 3%, polydimethylsiloxane 2%, ethyl hydroxybenzoate 0.1% are the quality percentage composition.
The C phase: Corbiculida peptide 2 %, sharkskin collagen peptide 6%, Eucheuma striatum polysaccharide 0.2%, Hijiki polysaccharide 0.3%, Acipenser Sinensis chondroitin sulfate 1%, water soluble Margarita ultrafine powder 5% are the quality percentage composition.
The D phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.1%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: heat respectively A mutually with B to 80 ℃, stir time slowly with B be added to A mutually in.Stirring is cooled to 45 ℃, C is added in the mixture of A, B phase, mix 10min after, add the D phase, mix homogenizing and make and be used to nurse the marine organisms function cosmetic emulsion of skin near the eyes.
According to the method for preparing the seashells bioactive peptide in the embodiment 3, enzymolysis makes active pearl shell peptide powder and scallop active peptide powder.
The A phase: HANSHENGJIAO 0.4%, acrylic ester/C10-30 alkyl acrylate cross-linked polymer 0.6%, EDTA-2Na0.1%, glycerol 5%, propylene glycol 5%, butanediol 5%, deionized water 38.5% are the quality percentage composition.
The B phase: active pearl shell peptide 0.2%, scallop active peptide 0.3%, collagen from squid skin peptide 10%, sargassan 1%, Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) polysaccharide 2%, Eucheuma gelatinosum polysaccharide 2%,
fish chondroitin sulfate 1%, tilapia chondroitin sulfate 1%, Margarita ultrafine powder 0.2%, instant Margarita powder 0.3%, deionized water 26% are the quality percentage composition.
The C phase: PEG-40 hydrogenated castor grease 1%, triethanolamine 0.2%, essence 0.1%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: mix the A phase component and be heated to 80 ℃ stirring, adding is pre-mixed uniform B phase when being cooled to 45 ℃, stirs 10min to transparent, makes behind the adding C phase stirring and evenly mixing to be used to nurse the marine organisms function cosmetic gel of skin near the eyes.
Embodiment 9
According to the method for preparing the seashells bioactive peptide in the embodiment 4, enzymolysis makes mussel active peptide powder.
A phase: HANSHENGJIAO 0.2%, acrylic ester/C
10-30Alkyl acrylate cross-linked polymer 0.6%, glycerol 5%, propylene glycol 3%, deionized water 45% are the quality percentage composition.
The B phase: mussel active peptide 3%, holothurian collagen peptide 2.8%, Porphyra Polysaccharide 0.5%, Stichopus japonicus chondroitin sulfate 0.5%, squid chondroitin sulfate 0.5%, Margarita ultrafine powder 1.7%, water soluble Margarita ultrafine powder 1%, deionized water 34.8% are the quality percentage composition.
The C phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.3%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: mix the A phase component and be heated to 80 ℃ stirring; Add when being cooled to 45 ℃ and be pre-mixed uniform B phase; Stir homogenizing 3min, be sequentially added into C each component mutually under the room temperature, stirring to make is used to nurse the marine organisms function cosmetics gel of skin near the eyes.
According to the method for preparing the seashells bioactive peptide in the embodiment 1, enzymolysis makes active pearl shell peptide powder.
The A phase: glycerol 5%, propylene glycol 3%, butanediol 2%, deionized water 50% are the quality percentage composition.
The B phase: the active pearl shell peptide 0.05% of method for preparing, commercially available oyster active peptides 0.05%, cod collagen peptide 0.02%, collagen from squid skin peptide 0.08%, Sargassum polysaccharide 0.02%, Thallus Laminariae polysaccharide 0.03%,
fish chondroitin sulfate 0.01%, pearl hydrolysate liposome 0.2%, deionized water 38.24% are the quality percentage composition.
The C phase: azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.2%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1% are the quality percentage composition.
Technical process: mix earlier the A phase, more in advance the B of mixing group add A mutually in, mix the back and add the C phase component, mixing makes and is used to nurse the marine organisms function cosmetics water preparation of skin near the eyes.
With following various samples promote blood microcirculation experiment (because skin position of living in is very special near the eyes, thus select Mice Auricle skin for use alternatively, to check the improve effect of cosmetics to its blood microcirculation)
Blank group: normal saline.
Sample 1 (allantoin among the a-f, ubiquinone
10, V
CMagnesium phosphorate, V
EThe total content of acetate is a mass fraction 10%):
A: allantoin 0.05%, ubiquinone
101%, V
CMagnesium phosphorate 5%, V
EAcetate 3.95%, normal saline 90%;
B: allantoin 0.1%, ubiquinone
100.9%, V
CMagnesium phosphorate 4%, V
EAcetate 5%, normal saline 90%;
C: allantoin 0.2%, ubiquinone
100.8%, V
CMagnesium phosphorate 4.5%, V
EAcetate 4.5%, normal saline 90%;
D: allantoin 0.3%, ubiquinone
100.7%, V
CMagnesium phosphorate 5%, V
EAcetate 4%, normal saline 90%;
E: allantoin 0.5%, ubiquinone
101%, V
CMagnesium phosphorate 5%, V
EAcetate 3.5%, normal saline 90%;
F: allantoin 0.6%, ubiquinone
100.01%, V
CMagnesium phosphorate 4.39%, V
EAcetate 5%, normal saline 90%.
Sample 2 (the seashells bioactive peptide among the A1-F, marine animal collagen peptide, Sargassum polysaccharides, chondroitin sulfate, Margarita powder, allantoin, ubiquinone
10, V
CMagnesium phosphorate, V
EThe total content of acetate is mass fraction 10%, wherein A
1-A
6Difference only be that the kind of seashells bioactive peptide is different):
A
1, Paphia Undulata peptide 2 %, cod collagen peptide 2%, sodium alginate 1%, shark chondroitine 0.2%, instant Margarita powder 2.7%, allantoin 0.1%, ubiquinone
100.5%, V
CMagnesium phosphorate 0.5%, V
EAcetate 1%, normal saline 90%;
A
2, Corbiculida peptide 2 %, other same A
1
A
3, active pearl shell peptide 2%, other same A
1
A
4, scallop active peptide 2%, other same A
1
A
5, mussel active peptide 2%, other same A
1
A
6, oyster active peptides 2%, other same A
1
B, Corbiculida bioactive peptide 1%, sharkskin collagen peptide 1%, Eucheuma striatum polysaccharide 0.2%, Hijiki polysaccharide 0.3%, Acipenser Sinensis chondroitin sulfate 1%, water soluble Margarita ultrafine powder 2.9%, allantoin 0.6%, ubiquinone
101%, V
CMagnesium phosphorate 1%, V
EAcetate 1%, normal saline 90%;
C, active pearl shell peptide 1%, scallop active peptide 1%, collagen from squid skin peptide 1%, sargassan 0.5%, Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) polysaccharide 0.5%, Eucheuma gelatinosum polysaccharide 0.1%,
Fish chondroitin sulfate 0.5%, tilapia chondroitin sulfate 0.5%, Margarita ultrafine powder 1.1%, instant Margarita powder 0.3%, allantoin 0.3%, ubiquinone
100.2%, V
CMagnesium phosphorate 1%, V
EAcetate 2%, normal saline 90%;
D, mussel active peptide 2%, holothurian collagen peptide 1%, Porphyra Polysaccharide 0.2%, Stichopus japonicus chondroitin sulfate 0.2%, squid chondroitin sulfate 2%, Margarita ultrafine powder 0.4%, water soluble Margarita ultrafine powder 0.6%, allantoin 0.5%, ubiquinone
100.1%, V
CMagnesium phosphorate 1%, V
EAcetate 2%, normal saline 90%;
E, oyster active peptides 2.5%, active pearl shell peptide 0.5%, cod collagen peptide 2%, collagen from squid skin peptide 0.8%, Sargassum polysaccharide 0.2%, Thallus Laminariae polysaccharide 0.3%,
Fish chondroitin sulfate 1%, pearl hydrolysate liposome 2.2%, allantoin 0.2%, ubiquinone
100.1%, V
CMagnesium phosphorate 0.1%, V
EAcetate 0.1%, normal saline 90%;
F, Paphia Undulata peptide 2 %, active pearl shell peptide 1%, cod collagen peptide 2%, sodium alginate 1%, shark chondroitine 0.2%, water soluble Margarita ultrafine powder 2%, allantoin 0.1%, ubiquinone
100.5%, V
CMagnesium phosphorate 0.5%, V
EAcetate 0.7%, normal saline 90%.
Choose 72 healthy ICR mices cleaning levels, female, body weight 18-22g is divided into 18 groups at random, i.e. blank group, sample 1 group of (a-f), 2 groups in sample (A1-F), 4 every group.After the anesthesia of the pentobarbital sodium of lumbar injection 80mg/kg mice body weight, on the micro-tester of WX-6 type microcirculation, measure Mice Auricle capillary vessel number before the administration, thin vein caliber, arteriole caliber and arteriole VPV.Each group is carried out tail vein injection respectively, and the administration volume is a 0.2mL/10g mice body weight.After administration, when 5min, 15min, 30min, 45min, 60min, measure above-mentioned each item index of Mice Auricle same area.The index determining method: arteriole caliber, thin vein caliber are directly read in eyepiece micrometer.Capillary vessel number is that eyepiece micrometer is horizontal, the square root of vertical coordinate total length (1.0mm) the capillary vessel number product of cutting.VPV is divided into linear flow (5 minutes), line grain stream (4 minutes), grain linear flow (3 minutes), grain stream (2 minutes), unhurried current (1 minute) and arrheas (0 minute) by field Niu Shi sxemiquantitative integration method, and integration is big more, and flow velocity is fast more.Experimental result sees shown in Fig. 1 a, 1b, 1c, 1d, 2a, 2b, 2c, 2d, 3a, 3b, 3c, 3d, 4a, 4b, 4c and the 4d that wherein " sample 1 " group is the meansigma methods of a-f group; " sample 2 " group is the meansigma methods of A-F group; Sample " A " group is the meansigma methods of A1-A6.
By finding out in the above-mentioned accompanying drawing; Compare for 1 group with blank group, sample, sample can significantly improve the diameter of normal mouse auricula blood microcirculation for 2 groups, and the interior capillary vessel number of same field of view scope is obviously increased; Arteriole and thin vein caliber significantly enlarge, and the arteriole flow velocity is also obviously accelerated.Experimental result shows, marine bioactivity material in the cosmetics of the present invention and allantoin, ubiquinone
10, V
CMagnesium phosphorate, V
EAcetate has good collaborative complex role; Can make it improve the blood microcirculation effect is significantly strengthened; And can effectively promote blood microcirculation, active cell, alleviation eye fatigue near the eyes, the skin problem effect is obvious near the eyes to improving black eye, eyeprint, eye pouch and edema etc.
Tail vein injection sample A
1-A
6Experimental result show that the sample that contains the Paphia Undulata bioactive peptide (is sample A
1) to improve Mice Auricle blood microcirculation effect best, its Mice Auricle capillary vessel number, arteriole and thin vein caliber, arteriole flow velocity increasing degree also obviously are superior to other sample.This explanation is in cosmetics of the present invention, and the Paphia Undulata bioactive peptide is optimum seashells bioactive peptide.
Following marine organisms function cosmetics are carried out human body experiment on probation (because skin is very tender and lovely, weak near the eyes, and elasticity experiment needs stretched skin, so selection arm skin substitutes so that the improve effect of cosmetics to skin elasticity near the eyes to be described):
Cosmetic base: HANSHENGJIAO 0.2%, acrylic ester/C
10-30Alkyl acrylate cross-linked polymer 0.6%, glycerol 5%, propylene glycol 3%, azone/PEG40 castor oil hydrogenated 1%, triethanolamine 0.3%, phenoxyethanol/methyl hydroxybenzoate/butyl hydroxybenzoate/ethyl hydroxybenzoate/propyl hydroxybenzoate/oxybenzene isobutyl ester 0.1%, deionized water 89.8%.
The cosmetics that do not contain the marine bioactivity composition: allantoin 0.1%, ubiquinone
100.9%, V
CMagnesium phosphorate 4%, V
EAcetate 5%, cosmetic base 90%.
Sample 1-5: be successively prepare among the embodiment 1-5 be used to nurse the marine organisms function cosmetics of skin near the eyes.
The cosmetics (total content of seashells bioactive peptide, marine animal collagen peptide, Sargassum polysaccharides, chondroitin sulfate, Margarita powder is a mass fraction 10%) that only contain the marine bioactivity composition: embodiment 9 preparation be used to nurse the marine organisms function cosmetics of skin near the eyes.
Sample 6 (seashells bioactive peptide, marine animal collagen peptide, Sargassum polysaccharides, chondroitin sulfate, Margarita powder, allantoin, ubiquinone
10, V
CMagnesium phosphorate, V
EThe total content of acetate is a mass fraction 10%, and the kind of the seashells bioactive peptide that the difference of a-f only is to be added is different):
A, Paphia Undulata peptide 2 %, cod collagen peptide 2%, sodium alginate 1%, shark chondroitine 0.2%, instant Margarita powder 2.7%, allantoin 0.1%, ubiquinone
100.5%, V
CMagnesium phosphorate 0.5%, V
EAcetate 1%, cosmetic base 90%;
B, Corbiculida peptide 2 %, other same a;
C, active pearl shell peptide 2%, other same a;
D, scallop active peptide 2%, other same a;
E, mussel active peptide 2%, other same a;
F, oyster active peptides 2%, other same a.
Choose black eye are arranged, 56 of the healthy volunteers of skin problem near the eyes such as eyeprint, eye pouch, edema; Wherein the male is 14,42 of women, age 18-65 year; On average (28.7 ± 4.9) year; Do not have serious systemic disease, immunodeficiency or autoimmune disease, cosmetics are not had allergy, facial NAI, do not use hormone medicine or immunosuppressant, do not participate in other clinical trials, do not smear external preparation.Be divided into 14 groups at random, promptly the cosmetic base group, do not contain marine bioactivity composition cosmetics group, only contain marine bioactivity composition cosmetics group, 1 group in sample, 2 groups in sample, 3 groups in sample, 4 groups in sample, 5 groups in sample, 6 groups in sample (a-f), 4 every group.
Each organize the volunteer with the warm water cleaning skin after, evenly smear corresponding cosmetics at skin, arm skin (same position) near the eyes, every day each 1 time sooner or later, used for 4 weeks continuously.Use moisture of skin measuring instrument Corneometer CM825 to measure the water content in the keratodermatitis near the eyes, spectrophotometric color measurement instrument CM2600D tests near the eyes skin color, and (L-value can react the black and white degree of skin; L-value is big more; Show that the colour of skin is white more), Robo skin analyzer CS50 tests near the eyes wrinkle of skin (texture degree value is big more, show that wrinkle of skin is more, wrinkle is dark more); (result is with 1 exponential representation for skin elasticity tester MPA580 test arm skin elasticity; Unit is 1, and numerical value is big more, explains that skin elasticity is strong more).Survey keratodermatitis water content, color and wrinkle and arm skin elasticity near the eyes weekly 1 time.The result sees shown in Fig. 5 a, 5b, 6a, 6b, 7a, 7b, 8a and the 8b that wherein " sample 6 " group is the meansigma methods of a-f group.
Shown in above-mentioned accompanying drawing; Compare with " cosmetic base " group, " not containing marine bioactivity composition cosmetics " group, " cosmetics that only contain the marine bioactivity composition " group; Smearing " sample 1-5 " after the 3rd week; The keratodermatitis water content significantly increases near the eyes, skin color obviously bleaches, wrinkle shoals even complete obiteration, and skin elasticity has also obtained remarkable increase.Experimental result shows, the marine organisms function cosmetics among the present invention nurse that skin effects is distinguished near the eyes, wherein marine bioactivity material and allantoin, ubiquinone
10, V
CMagnesium phosphorate and V
EAcetate has the collaborative complex role of good forward, and effects such as xerosis cutis, pigmentation, cutis laxa, eyeprint significantly strengthen to make it improve near the eyes.
The experimental result of smearing sample a-f shows that after the 4th week, the improvement situation of keratodermatitis water content, color, wrinkle and skin elasticity is the most excellent near the eyes having smeared the cosmetics (being a group in the sample 6) that contain the Paphia Undulata bioactive peptide.This explanation is in cosmetics of the present invention, and the Paphia Undulata bioactive peptide is optimum seashells bioactive peptide.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included in protection scope of the present invention.
Claims (10)
1. one kind is used to nurse the marine organisms function cosmetics of skin near the eyes, it is characterized in that: in marine organisms function cosmetics gross mass, contain the active component of following quality percentage composition:
Seashells bioactive peptide 0.1-10%
Marine animal collagen peptide 0.1-10%
Sargassum polysaccharides 0.05-5%
Chondroitin sulfate 0.01-2%
Margarita powder 0.2-15%.
2. according to claim 1ly be used to nurse the marine organisms function cosmetics of skin near the eyes, it is characterized in that: in marine organisms function cosmetics gross mass, the quality percentage composition of each active component is:
Seashells bioactive peptide 0.5-6%
Marine animal collagen peptide 0.5-6%
Sargassum polysaccharides 0.1-1%
Chondroitin sulfate 0.05-1%
Margarita powder 0.5-5%.
3. according to claim 1ly be used to nurse the marine organisms function cosmetics of skin near the eyes, it is characterized in that: it also contains the active component of following quality percentage composition:
Allantoin 0.05-0.6%
Ubiquinone
100.01-1%
V
CMagnesium phosphorate 0.1-5%
V
EAcetate 0.1-5%.
4. each describedly is used to nurse the marine organisms function cosmetics of skin near the eyes according to claim 1-3; It is characterized in that: described seashells bioactive peptide prepares through the method that contains following operation: ocean shellfish meat behind protease hydrolyzed, is promptly got through the ultrafiltration of 3000Da ultrafilter membrane again; Wherein said seashells comprises one or more in Paphia Undulata, Corbiculida, Margarita, scallop, mussel and the Concha Ostreae, and described protease comprises one or more in bromelain, papain, neutral protease, alkaline protease, pepsin, trypsin and the subtilisin.
5. according to claim 4ly be used to nurse the marine organisms function cosmetics of skin near the eyes, it is characterized in that: described seashells is a Paphia Undulata.
6. each describedly is used to nurse the marine organisms function cosmetics of skin near the eyes according to claim 1-3, and it is characterized in that: described marine animal collagen peptide comprises one or more in cod collagen peptide, sharkskin collagen peptide, holothurian collagen peptide and the collagen from squid skin peptide.
7. each describedly is used to nurse the marine organisms function cosmetics of skin near the eyes according to claim 1-3, and it is characterized in that: described Sargassum polysaccharides comprises one or more in sodium alginate, Porphyra Polysaccharide, Sargassum polysaccharide, Thallus Laminariae polysaccharide, sargassan, Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) polysaccharide, Eucheuma gelatinosum polysaccharide, Eucheuma striatum polysaccharide and the Hijiki polysaccharide.
8. each describedly is used to nurse the marine organisms function cosmetics of skin near the eyes according to claim 1-3; It is characterized in that: described chondroitin sulfate is the extract of marine animal cartilage, and wherein said marine animal is one or more in shark, Acipenser Sinensis,
fish,
fish, tilapia, Stichopus japonicus and the squid.
9. each describedly is used to nurse the marine organisms function cosmetics of skin near the eyes according to claim 1-3, and it is characterized in that: said Margarita powder comprises one or more in Margarita ultrafine powder, water soluble Margarita ultrafine powder, instant Margarita powder and the pearl hydrolysate liposome.
10. each describedly is used to nurse the marine organisms function cosmetics of skin near the eyes according to claim 1-3, and it is characterized in that: said marine organisms function cosmetics comprise cream frost, emulsion, gel, gel or water preparation; Described cosmetics can be used as ordinary cosmetics or have the function cosmetics of nursing skin effect near the eyes.
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