Summary of the invention
The object of the present invention is to provide a kind of red soil soil property with organic fertilizer produced from sludge generated composition prescription and preparation method, this fertilizer utilization sludge fermentation is processed, and can make full use of mud, turns waste into wealth.
Technical scheme provided by the invention is following:
A kind of red soil soil property organic fertilizer produced from sludge generated, this fertilizer is formed by sludge fermentation, may further comprise the steps:
A, mud is adjusted to moisture content 55-65%, C/N value 27-33, full carbon 35-45%;
B, fermented manure for the first time: deliver to fermentation unit after stirring and build up fermentation heap, heat up, at leavening temperature 52-57 ℃, under the condition of pH value 9-11, fermentation 65-75h;
C, fermented manure for the second time: at leavening temperature 58-63 ℃, under the condition of pH value 7-9, fermentation 55-75h.
Wherein, the mud of described mud for using alkaline flocculation agent to handle, described mud can be from sewage work, livestock breeding industry etc.In sewage treatment process; Its technology is divided into adds alkaline flocculation agent processing and does not add two kinds of technologies of alkaline flocculation agent processing; Alkalescence flocculation agent such as CaO etc. are used for the granule foreign of sedimentation sewage etc.; The mud of mud that the present invention is used for using alkaline flocculation agent to handle, its pH value meta-alkalescence.
More preferably, the fermented manure temperature is 55 ℃ for the first time, PH9.7, time 71h.After the fermentation, the pH value of compost descends naturally.
More preferably, the fermented manure temperature is 60 ℃ for the second time, pH value 8.7, time 65h.
In preferred embodiment of the present invention, compost can adopt composting devices such as fermentation vat formula, storehouse formula or tunnel like.These composting devices are that prior art and can buying obtains.
In preferred embodiment of the present invention, steps A be adjusted into the adding agricultural organic waste, described agricultural organic waste comprises a kind of or its mixture in culture medium of edible fungus tankage, tobacco leaf slag, corn cob, the agricultural crop straw etc.
In preferred embodiment of the present invention, add the amendment that becomes thoroughly decomposed among step B and the step C respectively, the add-on of the amendment that becomes thoroughly decomposed is: by weight, 8-15% compost weight.The effect of amendment of becoming thoroughly decomposed is for organism of fermentation better growing environment to be provided.
In preferred embodiment of the present invention, the amendment that becomes thoroughly decomposed that adds among the step B, the amendment that becomes thoroughly decomposed that adds among the step B, its main component comprises soybean cake powder 27-31%, starch 68-72%, KH
2PO
40.8-1.2% is modulated to moisture 44-46%, PH7.1-7.3; The amendment that becomes thoroughly decomposed that adds among the step C, its main component comprise soybean cake powder 19-23%, starch 76-80%, KH
2PO
40.8-1.2% is modulated to moisture 50-54%, PH 7.3-7.6.These compositions of the present invention are decided according to the nourishing and growing of different microorganisms in different fermentations stage, can allow organism of fermentation fully grow.
In preferred embodiment of the present invention, after fermented manure finishes for the first time, before the fermented manure, compost is carried out complete stirring for the second time.Stirring is handled and can be promoted raw material to contact with air, strengthens the vigor of microorganism in the aerobic fermentation heap body, thereby improves composting efficiency and compost quality.
The present invention can also add and be used to the biotechnological formulation that becomes thoroughly decomposed that ferments in right amount in step B and/or step C.Contain microorganisms such as corrupt clostridium in these preparations.These preparations are commercialization at present, can buy from market to obtain.These biotechnological formulations can strengthen or the biological inoculum of afterfermentation, regulate the flora structure of compost in producing.In an embodiment of the present invention, then be to utilize microorganism self-contained in the mud to ferment, do not add the biotechnological formulation that becomes thoroughly decomposed separately.
The application of aforementioned organic fertilizer, it is applied to the growth of Hylocereus undatus.
At present, report shortens the research of compost fermentation time both at home and abroad, in large-scale aerobic buttress, tunnel fermentation, adds aspect the microbial inoculum and the processing of taking grain out of a granary to sun it mostly.Two step method static compost zymotechnique provided by the invention, that is: one-step fermentation compost temperature 52-57 ℃, 58-63 ℃ of two stage fermentation compost temperature; Twice compost temperature fully covered the multiple high temperature microbial growth temperature range of becoming thoroughly decomposed; Adjust its air flow according to full carbon variation, temperature variation, the rate of emergence index of substrate digest process in real time again; Thereby the mud of a kind of less investment is provided, technology is simple and easy, fermentation period is short, occupation of land is few is compost method on the spot.
The present invention adopts two step aerobic composts, and the 30-40 days time of becoming thoroughly decomposed of traditional bar being piled up neatly sludge composting method is reduced to 110-160h, shortens fermentation period, has improved composting efficiency greatly, has improved the processing power of mud.The present invention is applicable to that places such as medium and small mud factory and livestock culturing field use the high-rate composting of alkaline flocculation agent, owing to need not to concentrate compost, has reduced the secondary pollution of processes such as transportation.Because leavening temperature of the present invention is high, at 52-63 ℃, can effectively remove pathogenic agent, parasitic ovum, so the organic fertilizer that the present invention processes can be used safely.And organic fertilizer meta-alkalescence of the present invention is suitable for improving the red soil soil property, and the Hylocereus undatus growth that especially grows in the red soil soil property is used.
Embodiment
The preparation of embodiment one fertilizer
1 materials and methods
1.1 material, equipment
The amendment 1.1.1 become thoroughly decomposed: become thoroughly decomposed amendment M501, MA502, M501 are mainly by soybean cake powder 29%, starch 70%, KH
2PO
41% constitutes, and is modulated to moisture 45%, PH7.1-7.3; MA502 is mainly by soybean cake powder 21%, starch 78%, KH
2PO
41% constitutes, and is modulated to moisture 52%, PH7.3-7.6.
1.1.2 substratum, composting material: beef-protein medium; Improvement Gause I substratum; The Ma Dingshi substratum, substratum that bacterium, fungi, actinomycetic starch hydrolysis experiment, gelatin hydrolysis test, cellulase activity are measured and counting substratum thereof.Domestic sludge is from stone Weihe, Xiamen sewage work, and it adopts alkaline flocculation agent to handle, moisture 59~81%, full carbon 52% (butt), full nitrogen 1.9% (butt); Mushroom soil is from the green mark biotechnology in Xiamen company (the culture medium of edible fungus tankage add amendments such as an amount of tobacco leaf slag), moisture 21~29%, full carbon 30~39% (butt), full nitrogen 11% (butt).Mud and mushroom soil are made into 13: 7 (w/w), full carbon 41%, moisture 60%, C/N value 27~33, as composting material.
1.1.3 plant and instrument: the WDP microorganism is used incubator more, intelligent illumination box, counting apparatus for bacteria, colony counter and plastics tray etc.The long 4.6m of storehouse formula compost reactor, wide 2.0m, high 2.1m, configuration air quantity 21m
3The calorifier of/min (normal temperature to 90 ℃) and time rly. are available from the green mark biotechnology in Xiamen company.Wherein, bottom 0.2m height cushions, leads pollution layer as air-blowing, air-flow, so that distinguished and admirable equably through ventilating hole channel entering in-vessel composting system, and can collect percolate it is discharged; Bulkhead is processed with warming plate, and does corrosionproof protection, continue to use 2 years still intact; About 200m is taken up an area of in the compost workshop of ten reactors
2, about 1500 tons of year processing mud.
1.2 method
1.2.1 process of the test: this test continued to carry out from year November in February, 2009 to 2010, used storehouse formula compost reactor, adopted the technology mode of two step method static fermentation compost, forced ventilation and temperature control.During the one-step fermentation compost, add 8-15% (w/w) the amendment MA502 that becomes thoroughly decomposed; During the two stage fermentation compost, add 8-15% (w/w) the amendment M501 that becomes thoroughly decomposed.Temperature, ventilation by calorifier, temperature controller, time relay control fermented manure.Get the equivalent sample respectively at each point for measuring temperature and carry out mixing, sample is divided into 2 parts, and portion is bright appearance, is stored in 4 ℃ of refrigerators, is used for mensuration and the bacteria suspension preparation of water ratio etc., and another duplicate samples dry storage is subsequent use.
1.2.2 the mensuration of the optimum temps of one-step fermentation compost, pH value and growth curve thereof: with composting material, respectively culture temperature (℃) be 50,52,55,58,60 next step storehouse formula static composts, accurately pipette sample under the 75h.Each is handled and repeats (down together) for 6 times.The pH of raw material is adjusted to 8.7,9.0,9.5,9.7,10.5,11.0,11.5 respectively, and 55 ℃ of next step storehouse formula static composts of temperature accurately pipette sample under the 71h.The mensuration of one step storehouse formula static compost growth curve is 5,10,15,20,24,28,33,36,40,45,50,55,60,65,70,75,80 times at incubation time (h), accurately pipettes sample.Measure bacterium, actinomycetes and fungi quantity and total count thereof respectively with microscope direct-counting method, the method for plate culture count; Measure full carbon with potassium bichromate titrimetric method, measure full nitrogen with the semimicro Kjeldahl determination
[22]Measure total count and carry out data analysis, confirm its optimum temperature range and pH value through DPS software.
1.2.3 the optimum temps of two stage fermentation compost and the mensuration of growth curve thereof: with the composting material of pH9.7, at 55 ℃ of next step storehouse formula static composts; With the complete stirring of composting material, carry out two step storehouse formula static composts at 71h, continue respectively temperature (℃) be 55,58,60,63,65, water ratio 50% time accurately pipettes sample during 65h.The mensuration of two step storehouse formula static compost growth curves is the same.Detection method is the same, confirms the optimum temps of two stage fermentation compost with this.
1.2.4 the mensuration of the best air flow of two step method static fermentation compost: with the composting material of pH9.7, at 55 ℃ of next step storehouse formula static composts, blowing that circulates (hot blast in good time) and blow off wind.At 71h with the complete stirring of composting material, at 60 ℃ of following two step storehouse formula static composts, at 71h to 136h time period circulate blowing (in good time hot blast) and blow off wind.Confirm its best air flow according to the full carbon variation of substrate digest process, temperature variation, rate of emergence index.Rate of emergence index (GI) is measured
[6]Adopt cucumber seeds (middle section 968).
2 results and analysis
2.1 the mensuration of the optimum temps of fermented manure, pH value and flora growth curve thereof
Temperature effect microbial growth, existence; Only under suitable temperature, the microorganism-decomposing microbial inoculum just can have the effect of stronger decomposing organic matter.Experiment shows that between 52 ℃~58 ℃, growing microorganism is all very fast, and total count is 1.4 * 10
7Individual/g~2.1 * 10
7Individual/g; The full carbon of substrate reduces to 34~36%.The one-step fermentation optimum growth temperature that can confirm this flora thus is 55 ℃.Equally, can confirm the growth conditions of two stage fermentation.
Influence that the factor of heap temperature mainly contains in the composting process, composting material, moisture content, organic content, pH value, C/N value, air flow, unit weight, hole and variation of ambient temperature rate etc.; And the solid heat conductance is poor, and on the heat production peak, heap temperature sharply rises, easily the appearance temperature gradient.Research shows that the temperature monitoring that reactor is good can improve the success ratio of compost and the quality of composting production with control.Therefore, the storehouse formula compost reactor of this test adopts cross-ventilated thermolysis to come the accumulation of balance microbial metabolism activity heat production.
2.2 the mensuration of the best air flow of two step method static fermentation compost
Bar buttress aerobic compost volume is bigger, the temperature of compost, the more bad control of oxygen level, and for this reason, the design of the compost reactor volume of this test is moderate, can allow air evenly contact fully with raw material.Experiment shows that at 0h~24h, oxygen requirement is less adjustment period of the compost bacterial strain, ventilation 3min, stops 25min, and the heap body does not need warm air just can be warmed up to 55 ℃, and it is identical that its heating curve and bar are piled up neatly aerobic compost.At 24h~71h, the compost bacterial strain is more in logarithmic phase, balance period oxygen requirement, need only adjust generally speaking aeration time just can controlled temperature about in the of 55 ℃, visible, it is similar that the temperature curve of a step composting process and bar are piled up neatly aerobic compost.The two step composting process designs of this test are based on temperature curve and the correction of strain growth condition of bar buttress aerobic compost.At 71h~113h, this moment, nutritive ingredient such as water-soluble organic carbon reduced, become thoroughly decomposed the heat energy that produces also deficiency keep become thoroughly decomposed 60 ℃ of temperature of substrate, at this moment just need a spot of warm air.In the compost later stage, the microbial metabolism activity is slowed down, and this storehouse formula compost reactor will make the heap temperature substantially constant through the heat effect of warm air.But also must consider the influence of convection of air, prevent the moisture excessive loss moisture evaporation.The result sees table 1.
The air flow experiment and the result of table 1 bin aerobic compost
Through above processing, after mud is transformed into organic fertilizer, can be used for agricultural fertilizer.
Embodiment two
A kind of sludge fermentation method may further comprise the steps:
A, mud is adjusted to moisture content 55%, full carbon ratio 45%, C/N value 27 with the culture medium of edible fungus tankage;
B, fermented manure for the first time: adjusted mud adds 10% (w/w) amendment that becomes thoroughly decomposed, and this its main component of amendment of becoming thoroughly decomposed comprises soybean cake powder 27%, starch 72%, KH
2PO
41%, be modulated to moisture 44%, PH7.1, deliver to fermentation unit after stirring and build up fermentation heap, heat up, 52 ℃ of leavening temperatures, under the condition of pH value 9.5, fermentation 80h;
C, fermented manure for the second time: the compost after the fermentation adds 12% (w/w) amendment that becomes thoroughly decomposed for the first time, and its main component comprises soybean cake powder 20%, starch 78.8%, KH
2PO
41.2%, be modulated to moisture 50%, PH 7.6, after turning is stirred, 63 ℃ of leavening temperatures, under the condition of pH value 8.7, fermentation 50h.
Other method of present embodiment and operation and embodiment one are basic identical.Through above processing, after mud is transformed into organic fertilizer, can be used for agricultural fertilizer.
Embodiment three
A kind of sludge fermentation method may further comprise the steps:
A is adjusted to moisture content 65%, full carbon ratio 40-44%, C/N value 33 with the culture medium of edible fungus tankage with mud;
B, fermented manure for the first time: adjusted mud adds 8% (w/w) amendment that becomes thoroughly decomposed, and this its main component of amendment of becoming thoroughly decomposed comprises soybean cake powder 31%, starch 68.2%, KH
2PO
40.8%, be modulated to moisture 46%, PH7.3, deliver to fermentation unit after stirring and build up fermentation heap, heat up, 57 ℃ of leavening temperatures, under the condition of pH value 10.5, fermentation 60h;
C, fermented manure for the second time: the compost after the fermentation adds 15% (w/w) amendment that becomes thoroughly decomposed for the first time, and its main component comprises soybean cake powder 23%, starch 76%, KH
2PO
41%, be modulated to moisture 50%, PH 7.6, after turning is stirred, 58 ℃ of leavening temperatures, under the condition of pH value 8, fermentation 80h.
Other method of present embodiment and operation and embodiment one are basic identical.Through above processing, after mud is transformed into organic fertilizer, can be used for agricultural fertilizer.
The application of embodiment 4 fertilizer
1. materials and methods
1.1 supply the examination material
Hylocereus undatus is the Taiwan Hylocereus undatus kind fruit that the Jiayi County from Taiwan draws in Fujian Institute of Subtroical Plant.
Hylocereus undatus (Hylocereus undatus) is claimed red dragon fruit, prickly pear, Huanglong fruit again, belongs to the Cactaceae hylocereus and belongs to (Hylocereus), is perennial climbing up by holding on to property succulent, and viticula is triangular shape; Originate in southern Mexico, all states in Central America; Bud and fruit edible.Nutritious because of it, output is high, and better economic benefit increases in ground cultivation amounts such as China Hainan, Fujian at present year by year.
Storehouse formula (life) mud fertilizer, storehouse formula pig manure, bar buttress (life) mud fertilizer, bar buttress pig manure, bar buttress rapeseed cake fertilizer all contain organic 30% (butt); By ring sewage disposal company, the development of the green mark biotechnology in Xiamen company in Fujian Academy of Agricultural Sciences, Fujian Institute of Subtroical Plant, the Xiamen water utilities, product meets national fertilizer quality and safety standards.Wherein, The raw material of storehouse formula mud fertilizer, storehouse formula pig manure, bar buttress formula mud fertilizer is from ring sewage disposal company and the green mark biotechnology in Xiamen company in the water utilities of Xiamen; Production storehouse formula fertilizer adopts the two step method bin aerobic composting process among the embodiment 1 to 3, produces bar buttress fertilizer and adopts conventional bar buttress composting process.Mud fertilizer, pig manure, rapeseed cake fertilizer all contain organic matter 30%, N3.6%, P
2O
54.1%, K
2O3.3%, CaO 3.4%.
1.2 test design
Experimental field be located at Feng Nan farm, Xiamen City, Fujian Province, belong to the south subtropics weather; Supplying examination soil is red soil, organic C 10.3gKg
-1, full N 0.88gKg
-1, full P 0.87gKg
-1, full K 16.73gKg
-1, alkaline hydrolysis N72.49mgKg
-1, available P 32.38mgKg
-1, quick-acting K 121.6mgKg
-1, pH 5.1; Ni 0.55mgKg
-1, Cd 0.043mgKg
-1, Cr 0.59mgKg
-1In June, 2008, the Hylocereus undatus cuttage seeding was colonizated in plantation, took brick post and stone column, intercolumniation 2.5m, and around the high about 30cm of post mound, the about 1m of diameter is in case ponding.The cultivation of frame formula is carried out in every post kind 4 strains, observes the viticula growth and the situation of yielding positive results.Each handles 10 posts of planting, and selected at random 5 posts in every sub-district are seen table 1 as the observation strain.Each handles N, the P that number applies
2O
5, K
2The O nutrient is all identical; Other field management is undertaken by routine.
1.3 sample collecting and analytical procedure
Gradation was plucked receipts, was added up to survey and produce during fruit maturation in June, 2010 to November.Pluck the receipts phase in October, generated 4 sections on ripe nutrition stem, and carried out biochemical analysis in 1 year that all is handled respectively observe 4 of strain sampling fruits, cuts 10cm by conventional method; During last gather for 1 time, get 0~40cm soil sample and carry out soil nutrient and heavy metal analysis.All mensuration all repeat 3 times, get its mean value and carry out statistical study.
The test of table 2 Hylocereus undatus application of organic fertilizers
2 results and analysis
2.1 storehouse formula fertilizer is to the influence of the available nutrient of soil and Hylocereus undatus viticula carbon, nitrogen nutrient content
The soil of dispensing fertilizer, its water conservation, guarantor's fertilizer efficiency fruit obviously are better than chemical fertilizer of simple substance.Can know that from table 2 available nutrient of soil of application of organic fertilizers and Hylocereus undatus viticula carbon, the comparison of nitrogen nutrient all have according to (not application of organic fertilizers) extremely significantly increases (P<0.01); Wherein, the fertilizer efficiency of a storehouse formula fertilizer and a bar muck does not have significant difference, and is slightly better than the fertilizer efficiency of rapeseed cake fertilizer; Pig manure, mud fertilizer all are to be raw material with animal excrements, contain the required various nutritive substances of abundant organic matter and crop, belong to the high-quality complete manure.Therefore, can think tentatively experimentally that it is feasible adopting two step method bin aerobic composting process that domestic sludge is changed into fertilizer from fertilizer efficiency.The characteristics of two step method bin aerobic composting process are, the high temperature bacterium leavening temperature that becomes thoroughly decomposed is controlled at 52 ℃-57 ℃, 58 ℃-63 ℃ two stages, has fully covered the growth temperature range of the microbiobacterial agent that becomes thoroughly decomposed, and therefore, the effect of becoming thoroughly decomposed can be better.
The confession of this experiment examination soil is red soil, and glutinous grain and iron in the soil, aluminium content are all higher, and organic content is lower, is unfavorable for the agglomeration and the microbial growth of soil; This experiment has improved the available nutrient of soil through the fertilizer of using meta-alkalescence, and Hylocereus undatus viticula carbon, nitrogen nutrient also are improved.
Table 3 storehouse formula fertilizer is to the influence of the available nutrient of soil and Hylocereus undatus viticula carbon, nitrogen nutrient content
Annotate: in butt.
2.2 storehouse formula fertilizer is to Hylocereus undatus growth and result's influence
Experiment shows that executing the Hylocereus undatus viticula growth of storehouse formula fertilizer, the quantity of blooming and output all extremely significantly increases (P<0.01) than executing having of chemical fertilizer of simple substance; And the fertilizer efficiency of a storehouse formula fertilizer and a bar muck does not have significant difference, and is slightly better than the fertilizer efficiency of rapeseed cake fertilizer; The result sees table 3.The flower and fruit thinning measure is not taked in this test, therefore fruit more, fruit is less, has influenced single fruit weight and output.
Table 4 storehouse formula fertilizer is to the Hylocereus undatus plant strain growth and the influence of yielding positive results
2.3 storehouse formula fertilizer is to the influence of dragon fruit pulp quality
The vegetation period nutrient supply directly influence its pulp quality.Experiment shows, the viticula carbon of dragon fruit pulp quality and Hylocereus undatus, nitrogen nutrient and the viticula growth relation of being proportionate; Use the qualities such as dragon fruit pulp moisture, sugar, protein and the significant difference that do not have of using a bar muck of storehouse formula fertilizer, the result sees table 4.The organic mass-energy of external source promotes the growth and breeding of soil, plant rhizosphere microbe, produces more nutrient supply root systems of plant and absorbs, thereby improved fruit quality.
Table 5 storehouse formula fertilizer is to the influence of dragon fruit pulp quality
Annotate: in fresh weight.
2.4 storehouse formula fertilizer is to the influence of dragon fruit pulp mineral nutrition and pulp
The difference of different soils character and fertilising can influence the ability that root system of plant absorbs mineral element.Can know that from table 5 the applying sludge fertilizer is compared with control group, improve Fe, Ca, the P content of dragon fruit pulp.
Table 6 storehouse formula fertilizer is to the influence of Hylocereus undatus mineral constituents
Annotate: pulp is in fresh weight, and soil is in butt.
The soil of Fujian Province's plantation Hylocereus undatus is red soil, sandy soil mostly, and routine needs many organic fertilizers.Storehouse formula fertilizer has good fertilizer efficiency to the plantation Hylocereus undatus, is embodied in the viticula growth, spends number, the number of bearing fruit, output, pulp quality all to be significantly higher than simple use inorganic fertilizer.
Storehouse formula fertilizer has improved the available nutrient of soil; Along with the intensive style development of China's livestock industry and carrying out of rural urbanization; On the one hand; A large amount of mud causes environmental pollution because of lacking reasonable utilization; On the other hand, the peasant lacks self-supporting factors such as farm manure and causes the farmland organic content on a declining curve.Two step method bin aerobic composting process less investment; Be suitable for the sludge disposal of small and medium cities sewage work, large-scale livestock-raising factory; The fertilizer of producing can increase the organic matter of soil, favourable plant-growth, thus embodied the theory of the eubiosis, recycling economy.
Above-mentioned only is specific embodiment of the present invention, but design concept of the present invention is not limited thereto, and allly utilizes this design that the present invention is carried out the change of unsubstantiality, all should belong to the behavior of invading protection domain of the present invention.