CN102308216B - 免疫球蛋白糖基化模式分析 - Google Patents

免疫球蛋白糖基化模式分析 Download PDF

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CN102308216B
CN102308216B CN201080007076.7A CN201080007076A CN102308216B CN 102308216 B CN102308216 B CN 102308216B CN 201080007076 A CN201080007076 A CN 201080007076A CN 102308216 B CN102308216 B CN 102308216B
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immunoglobulin
fragment
ides
glycosylation pattern
enzyme
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CN102308216A (zh
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H·科尔
J·T·雷古拉
P·松德尔曼
A·策克
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Roche Glycart AG
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Roche Glycart AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Water Supply & Treatment (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080007076.7A 2009-02-09 2010-02-05 免疫球蛋白糖基化模式分析 Active CN102308216B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09001757 2009-02-09
EP09001757.5 2009-02-09
PCT/EP2010/000710 WO2010089126A2 (fr) 2009-02-09 2010-02-05 Analyse de motif de glycosylation d'immunoglobuline

Publications (2)

Publication Number Publication Date
CN102308216A CN102308216A (zh) 2012-01-04
CN102308216B true CN102308216B (zh) 2014-07-09

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Country Status (8)

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US (2) US20110294150A1 (fr)
EP (1) EP2394173B1 (fr)
JP (1) JP5461586B2 (fr)
CN (1) CN102308216B (fr)
CA (1) CA2750977A1 (fr)
ES (1) ES2535963T3 (fr)
SG (1) SG173589A1 (fr)
WO (1) WO2010089126A2 (fr)

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WO2009080278A1 (fr) * 2007-12-21 2009-07-02 F. Hoffmann-La Roche Ag Essai de stabilité d'anticorps
CN103134874B (zh) * 2011-12-01 2015-05-20 成都康弘生物科技有限公司 一种测定蛋白中糖基的方法
CN103217499B (zh) * 2013-01-15 2015-12-02 珠海市丽珠单抗生物技术有限公司 一种测定免疫球蛋白电荷异构体的糖基化和末端修饰情况的方法
CN103217490B (zh) * 2013-01-15 2016-08-03 珠海市丽珠单抗生物技术有限公司 一种在发酵过程中测定免疫球蛋白的糖基化和末端修饰情况的方法
CN103217489B (zh) * 2013-01-15 2016-03-09 珠海市丽珠单抗生物技术有限公司 一种测定蛋白纯化工艺过程中样品的糖基化和末端修饰情况的方法
WO2014121031A1 (fr) * 2013-01-31 2014-08-07 Excelimmune, Inc. Caractérisation de mélanges d'anticorps par spectrométrie de masse
BR112016023046A8 (pt) 2014-04-04 2021-05-11 Mayo Found Medical Education & Res kit compreendendo anticorpos e agentes redutores
EP2975401B1 (fr) * 2014-07-18 2019-12-25 Hexal AG Procédé amélioré de mise en correspondance de glycanes de glycoprotéines dans des échantillons de sérum
GB201416849D0 (en) * 2014-09-24 2014-11-05 Genovis Ab Method
GB201502305D0 (en) 2015-02-12 2015-04-01 Hansa Medical Ab Protein
GB201502306D0 (en) 2015-02-12 2015-04-01 Hansa Medical Ab Protein
JP6968058B2 (ja) 2015-09-24 2021-11-17 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ 質量分析法による免疫グロブリン遊離軽鎖の同定
EP3523647B1 (fr) 2016-09-07 2024-06-26 Mayo Foundation for Medical Education and Research Identification et surveillance d'immunoglobulines clivées par masse moléculaire
US11021530B2 (en) * 2016-10-31 2021-06-01 Hexal Ag Antibody preparation
WO2018093868A1 (fr) * 2016-11-16 2018-05-24 University Of Florida Research Foundation, Inc. Protéases d'immunoglobulines, compositions et leurs utilisations
US11946937B2 (en) 2017-09-13 2024-04-02 Mayo Foundation For Medical Education And Research Identification and monitoring of apoptosis inhibitor of macrophage
EP3746472A2 (fr) * 2018-01-31 2020-12-09 Regeneron Pharmaceuticals, Inc. La glucuronylation comme modification post-translationnelle acide sur des anticorps monoclonaux therapeutiques
CN111936860A (zh) * 2018-04-10 2020-11-13 Dh科技发展私人贸易有限公司 质谱法中的抗体的自上而下分析
WO2019229058A1 (fr) * 2018-05-29 2019-12-05 Capsenze Biosystems Ab Procédé et système pour fournir des fragments d'anticorps convenant à un contrôle de qualité en ligne
CN110749736A (zh) * 2018-07-24 2020-02-04 中国科学院大连化学物理研究所 一种基于化学反应简化的O-GalNAc糖基化肽段鉴定方法
CN110865129B (zh) * 2018-08-27 2022-09-23 齐鲁制药有限公司 一种度拉鲁肽中多种修饰水平的检测方法
CN110045125B (zh) * 2019-04-03 2022-08-09 中国医学科学院北京协和医院 一种用于诊断腹膜后纤维化的生物标志物及其用途
BR112021023438A2 (pt) * 2019-05-23 2022-01-11 Regeneron Pharma Caracterização de variantes de carga específica de domínio de anticorpos
CN110632229A (zh) * 2019-09-24 2019-12-31 华兰生物工程重庆有限公司 高效液相色谱仪检测静注人免疫球蛋白(pH4)蛋白质含量的方法
EP4162250A4 (fr) * 2020-06-09 2024-10-23 Regeneron Pharma Procédé à haut rendement et à base de spectrométrie de masse pour quantifier des anticorps
IL301234A (en) * 2020-09-11 2023-05-01 Regeneron Pharma Methods for binding site identification using hydrogen exchange mass spectroscopy
CN114524859B (zh) * 2022-01-28 2024-07-12 黑龙江省农业科学院农产品质量安全研究所 水稻球蛋白的提取方法及液相检测方法
US12091694B2 (en) 2022-11-18 2024-09-17 Seismic Therapeutic, Inc. Fc fusion molecules and uses thereof

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WO2006068720A2 (fr) * 2004-11-12 2006-06-29 The Brigham And Women's Hospital, Inc. Polypeptides de ligands de selectine des cellules hematopoietiques et procedes d'utilisation de ces polypeptides
CN101257922A (zh) * 2005-09-09 2008-09-03 格利科菲公司 主要包含Man7GlcNAc2、Man8GlcNAc2糖形式的免疫球蛋白
CN101282990A (zh) * 2005-08-26 2008-10-08 阿雷斯贸易股份有限公司 糖基化干扰素-β的制备方法

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CN101158666B (zh) * 2006-10-08 2012-06-27 许洋 一种用含有抗体组的基质去捕获生物样品中生物标志的分析方法
CN101201337B (zh) * 2006-12-15 2012-06-13 首都师范大学 鉴定小麦高分子量谷蛋白亚基的生物质谱方法

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WO2006068720A2 (fr) * 2004-11-12 2006-06-29 The Brigham And Women's Hospital, Inc. Polypeptides de ligands de selectine des cellules hematopoietiques et procedes d'utilisation de ces polypeptides
CN101282990A (zh) * 2005-08-26 2008-10-08 阿雷斯贸易股份有限公司 糖基化干扰素-β的制备方法
CN101257922A (zh) * 2005-09-09 2008-09-03 格利科菲公司 主要包含Man7GlcNAc2、Man8GlcNAc2糖形式的免疫球蛋白

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Glycosylation profiling of a therapeutic recombinant monoclonal antibody with two N-linked glycosylation sites using liquid chromatography coupled to a hybrid quadrupole time-of-flight mass spectrometer;Amareth Lim et al.;《Analytical Biochemistry》;20080109;第375卷(第2期);163-172 *
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Therapeutic cleavage of IgG: new avenues for treating inflammation;Kutty Selva Nandakumar et al.;《Trends in Immunology》;20080306;第29卷(第4期);173-178 *

Also Published As

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US20180164325A1 (en) 2018-06-14
CA2750977A1 (fr) 2010-08-12
EP2394173B1 (fr) 2015-03-25
CN102308216A (zh) 2012-01-04
WO2010089126A2 (fr) 2010-08-12
JP2012517585A (ja) 2012-08-02
ES2535963T3 (es) 2015-05-19
US20110294150A1 (en) 2011-12-01
WO2010089126A3 (fr) 2010-11-11
JP5461586B2 (ja) 2014-04-02
SG173589A1 (en) 2011-09-29
EP2394173A2 (fr) 2011-12-14

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