CN102283155A - Method for culturing Stichopus japonicus seed - Google Patents

Method for culturing Stichopus japonicus seed Download PDF

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CN102283155A
CN102283155A CN2011101989347A CN201110198934A CN102283155A CN 102283155 A CN102283155 A CN 102283155A CN 2011101989347 A CN2011101989347 A CN 2011101989347A CN 201110198934 A CN201110198934 A CN 201110198934A CN 102283155 A CN102283155 A CN 102283155A
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stichopus japonicus
pond
water
seed ginseng
seed
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CN102283155B (en
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张榭令
邹安革
邹士方
代文汇
刘永旗
梁文恒
卢俊声
孙卫明
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Shandong Anyuan Seed Technology Co., Ltd
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PENGLAI ANYUAN AQUICULTURE CO Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention relates to a method for culturing a Stichopus japonicus seed, which belongs to the technical field of a Stichopus japonicus seed culturing method and comprises the following steps of (1) fishing and catching; (2) screening; (3) transporting and (4) culturing the Stichopus japonicus seed. According to the method for culturing the Stichopus japonicus seed disclosed by the invention, the culturing process is easy to operate, the Stichopus japonicus seed is scientifically selected and fished and caught in advance; the Stichopus japonicus seed can be matured in advance and lay eggs by utilizing the method of indoor heating and accelerating, thus, large-scale seeds can be cultured in the same year, and the produced Stichopus japonicus seed has enhanced hereditary capacity, strong resistance and good property; moreover, as the feed of the Stichopus japonicus seed and the water temperature and the water quality in the Stichopus japonicus culturing pond are strictly controlled, the fecundity of the Stichopus japonicus is better than that of the Stichopus japonicus cultured in the pond and the intertidal zone, thus, the laid eggs have good quality and the distortion ratio at each stage is high.

Description

A kind of breeding method of stichopus japonicus seed ginseng
Technical field
The present invention relates to a kind of breeding method of stichopus japonicus seed ginseng, belong to seed ginseng breeding method technical field.
Background technology
At present, nursery has the stichopus japonicus of pond culture in the selection of seed ginseng, the stichopus japonicus that culture the intertidal zone.Parent participation is laid eggs, though also can normal fertilization grow, distortion ratio and survival rate during to metamorphosis all descend greatly.Because the increasing of nursery man, the demand of seed ginseng strengthens, but stichopus japonicus heritability occurred and weakened through for many generations culturing, and resistance is poor, problems such as proterties degeneration.In addition, the stichopus japonicus that culture pond culture, intertidal zone, because be high density, intensive culture, so fish brood amount is not as good as the seed ginseng of natural waters, like this, the quality of the ovum of output own descends, and certainly will influence the distortion ratio of each phase.
Summary of the invention
The objective of the invention is to solve the weak point that above-mentioned prior art exists, provide a kind of cultivating process easy to operate, the seed ginseng fish brood amount height of being cultivated, and can the output proterties good, the breeding method of the stichopus japonicus seed ginseng of the seedling that distortion ratio is high.
The breeding method of a kind of stichopus japonicus seed ginseng of the present invention, its special character is may further comprise the steps:
1, adopts and catch
When the seawater water temperature of natural waters reaches 10-12 ℃, be that the sea area at 5-10m place is adopted and caught in the depth of water annual solar calendar November;
Adopted by diver diving and to catch, once adopting the quantity of catching can not be too much, and reducing as far as possible stimulates, avoid stichopus japonicus to be subjected to overstock and cause mechanical damage, adopt that used instrument can not contact oil stain in the process of catching, it is pure and fresh to keep supporting temporarily on the ship trough inner water matter, in time change water, adopt the stichopus japonicus of catching and avoid strong illumination;
The above-mentioned depth of water is the sea area at 5-10m place, require substrate to be husky mud and gravel at the bottom of, the good habitat that marine algae resources such as Zostera marina, mouse tail algae, sargassum are abundant;
2, screening
After seed ginseng is adopted and caught, carry out the germplasm quality testing, comprise the appearance index bodily form, corn, step etc.; Filter out that the bodily form is cylindrical, cucumber shape, the back side swell slightly, have on the back side that vertical 4-6 capablely differs in size, the conical corn (boss) of irregular arrangement, the outside of belly is smooth, sucker is intensive, be arranged in irregular 3 vertical bands, the above individualities of body weight 250g;
3, transportation
Use the wet fortune of canvas bucket, traffic density is no more than 300/m 3
4, the cultivation of seed ginseng
(1), the density of seed ginseng in the pond is controlled at 20-30 head/m 3
(2), the intensification accelerating of seed ginseng is cultivated, be that mid-January gone into the pond by solar calendar, the accelerating process will in a planned way make cultivation water heat up gradually, and to promote the gonad development of seed ginseng: water temperature was controlled at 2-3 ℃ after kind participated in the pond, kept 2-3 days, after this heat up every day 1 ℃, when being raised to 6 ℃, constant temperature was cultivated 6-8 days, after this heat up every day 0.5 ℃, constant temperature was cultivated 6-8 days when being raised to 10 ℃, after this heated up every day 0.5 ℃, and constant temperature is cultivated and waited to produce when being raised to 15 ℃;
(3), the throw something and feed mixture of powdery manufactured feed and ooze, the injected volume of this mixture accounts for the 3-8% of seed ginseng gross weight in the pond, the weight ratio of powdery manufactured feed and ooze is 1:(6-8), wherein the powdery manufactured feed comprises the batching of following percentage by weight: the sea-tangle dry powder 30-50% that comes unstuck, mouse tail algae dry powder 10-20%, sargassum dry powder 15-25%, hole sea lettuce dry powder 8-13%, fish meal 5-12%, dusty yeast 4-8%, B B-complex 1-3%, feed intake every night, the back of throwing something and feeding was changed water or flowing water in 1.5-2.5 hour;
(4), water quality management
Dissolved oxygen content in the water of control pond is 5-8ml/L, and change water 1-3 every day, and each quantity of exchanged water is the 40-60% of Chi Zhongshui, cultivate the purpose of ight soil, residual bait and rubbish in the pond to reach thorough removing, inhale end 1-2 time every day, fell the pond once in every 3-5 days, seed ginseng is laid eggs and was stopped down the pond in preceding 7 days; The not bait throwing in preceding 1 week of laying eggs;
(5), patrol the pond
As find that seed ginseng extremely swells, tentacle and sucker are lax, turn on the health, curl, stiff or part is individual when dirty phenomenon occurring arranging, illustrates that the dissolved oxygen content in the water of pond is on the low side, need change water or inflation immediately;
(6), the sign of laying eggs
Fishing for after seed ginseng enters the workshop, rush to stimulate the seed ginseng discharging, and need wait until that it indicates that female ginseng is about to lay eggs when having when laying eggs on a small quantity focused stimulation again, the activity of seed ginseng following behavior occur: the bull seed ginseng accumulates in Chi Jiao; The part seed ginseng moves to pool wall surface layer of water place fast; The body first half leaves pool wall, and that does not stop waves; The gonopore sealing, color burn; Existing seed ginseng discharges smart ovum in the pond, so far finishes seed ginseng cultivation work.
Described B B-complex is vitamin B1, B2, B6, B12, stablize among VC, nicotinic acid, folic acid, calcium pantothenate, inositol, Choline Chloride and vitamin A, D3, E, the K3 any two or more.
The breeding method of stichopus japonicus seed ginseng of the present invention, cultivating process is easy to operate, seed ginseng has been carried out the selection of science and adopts in advance and catch seed ginseng, utilize the way of indoor intensification accelerating, make the seed ginseng ripen in advance, lay eggs, can turn out large size seedling seed so then, the seedling heritability of output strengthens, strong stress resistance, and proterties is good, in addition, because the present invention is to the feeding of seed ginseng and the water temperature in the ginseng breed pond, water quality has been carried out strict control, so the seed ginseng fish brood amount is than the stichopus japonicus of pond culture, the stichopus japonicus that culture the intertidal zone is good, like this, the archiblast amount of output is good, the distortion ratio height of each phase.
Embodiment
Provide the specific embodiment of the present invention below with reference to accompanying drawing, the present invention is described further.
Embodiment 1
The breeding method of the stichopus japonicus seed ginseng of present embodiment may further comprise the steps:
1, adopts and catch
Adopt and catch season: annual solar calendar November, when the seawater water temperature of natural waters reaches 10-12 ℃, adopt catch the sea area the depth of water at 5-10m, at the bottom of substrate is husky mud and gravel, marine algae resources such as Zostera marina, mouse tail algae, sargassum are abundant, are the excellent environment of perching of wild stichopus japonicus;
Adopt and catch: adopt by diver's diving and catch, adopt when catching, should be noted that following item:
1. once adopting the quantity of catching can not be too much, and reducing as far as possible stimulates, and avoids stichopus japonicus to be subjected to overstock and causes mechanical damage;
2. diver, operating personnel on hand or ship, instrument etc. can not contact oil stain;
3. it is pure and fresh to keep supporting temporarily on the ship trough inner water matter, in time changes water;
4. adopt the stichopus japonicus that catches and avoid strong illumination;
2, screening
After seed ginseng is adopted and is caught, carry out the germplasm quality testing, mainly comprise appearance index (bodily form, corn, the step etc.) bodily form: cylindrical, the cucumber shape, swell slightly at the back side, there are vertical 4~6 row to differ in size on the back side, the conical corn (boss) of irregular arrangement, the outside of belly is smooth, and sucker is intensive, be arranged in not really 3 vertical bands of rule, the individuality that body weight 250g is above;
3, transportation
Use the wet fortune of canvas bucket, traffic density is no more than 300/m 3
2, seed ginseng is cultivated
(1), density: 20-30 head/m 3
(2), the intensification accelerating of seed ginseng is cultivated, be that mid-January gone into the pond by solar calendar, the accelerating process will in a planned way make cultivation water heat up gradually, and to promote the gonad development of seed ginseng: water temperature was controlled at 2-3 ℃ after kind participated in the pond, kept 2-3 days, after this heat up every day 1 ℃, when being raised to 6 ℃, constant temperature was cultivated 6-8 days, after this heat up every day 0.5 ℃, constant temperature was cultivated 6-8 days when being raised to 10 ℃, after this heated up every day 0.5 ℃, and constant temperature is cultivated and waited to produce when being raised to 15 ℃;
(3), the throw something and feed mixture of powdery manufactured feed and ooze, this mixture gets the 3-8% that injected volume accounts for seed ginseng gross weight in the pond, the weight ratio of powdery manufactured feed and ooze is 1:(6-8), wherein the powdery manufactured feed comprises the batching of following percentage by weight: the sea-tangle dry powder 30-50% that comes unstuck, mouse tail algae dry powder 10-20%, sargassum dry powder 15-25%, hole sea lettuce dry powder 8-13%, fish meal 5-12%, dusty yeast 4-8%, B B-complex 1-3%, feed intake every night, the back of throwing something and feeding was changed water or flowing water in 1.5-2.5 hour; B B-complex is vitamin B1, B2, B6, B12, stablize among VC, nicotinic acid, folic acid, calcium pantothenate, inositol, Choline Chloride and vitamin A, D3, E, the K3 any two or more;
(4), water quality management
Dissolved oxygen content in the water of control pond is 5-8ml/L, and change water 1-3 every day, and each quantity of exchanged water is the 40-60% of Chi Zhongshui, cultivate the ight soil in the pond, the purpose of residual bait to reach thorough removing, inhale end 1-2 time every day, fell the pond once in every 3-5 days, seed ginseng is laid eggs and was stopped down the pond in preceding 7 days; The not bait throwing in preceding 1 week of laying eggs.
(5), patrol the pond
The water quality situation: extremely swell as finding seed ginseng, tentacle and sucker are lax, turn on the health, curl, stiff or part is individual when dirty phenomenon occurring arranging, illustrates that DO is on the low side in the water of pond, need change water or inflation immediately;
(6) sign of laying eggs
Fishing for after seed ginseng enters the workshop, rush to stimulate the seed ginseng discharging, and need wait until that it indicates that female ginseng is about to lay eggs when having when laying eggs on a small quantity focused stimulation again, the activity of seed ginseng following behavior occur: the bull seed ginseng accumulates in Chi Jiao; The part seed ginseng moves to pool wall surface layer of water place fast; The body first half leaves pool wall, and that does not stop waves; The gonopore sealing, color burn; Existing seed ginseng discharges smart ovum in the pond, so far finishes seed ginseng cultivation work.
(7) 1. the seed ginseng individuality is big more, fish brood amount is big more, the maturity of ovum is good more for the specification of seed ginseng, so select the individual bigger seed ginseng of doing as far as possible.More than seed ginseng body weight 250 grams of natural waters.2. the full expansion of gonad development standard anatomic observation sexual gland, each branch of gonad are with reaching maturity and chap gradually, and main branch diameter can reach 1.5-3.0mm when ripe, and individual other can reach more than the 3.0mm.The female reproduction gland is salmon pink, and as seen ovum grain naked eyes can freely scatter after puncturing; The arrenotoky gland is milk yellow or milky, can flow out the milky refining after puncturing.Microscopically yolk and cytoplasm are transparent, and ovum particle shape shape is neat.
3. fish brood amount: individual average fish brood amount reaches 300~5,000,000, and minority is individual above 8,000,000.
Relatively seeing the following form of physical maturity seed ginseng and the inventive method artificial culture seed ginseng:
Figure 890881DEST_PATH_IMAGE001
The breeding method of a kind of stichopus japonicus seed ginseng of the present invention, cultivating process is easy to operate, seed ginseng has been carried out the selection of science and adopts in advance and catch seed ginseng, utilize the way of indoor intensification accelerating, make the seed ginseng ripen in advance, lay eggs, can turn out large size seedling seed so then, the seedling heritability of output strengthens, strong stress resistance, and proterties is good, in addition, because the present invention is to the feeding of seed ginseng and the water temperature in the ginseng breed pond, water quality has been carried out strict control, so the seed ginseng fish brood amount is than the stichopus japonicus of pond culture, the stichopus japonicus that culture the intertidal zone is good, like this, the archiblast amount of output is good, the distortion ratio height of each phase.

Claims (4)

1. the breeding method of a stichopus japonicus seed ginseng is characterized in that may further comprise the steps:
1), adopts and catch
When the seawater water temperature of natural waters reaches 10-12 ℃, be that the sea area at 5-10m place is adopted and caught in the depth of water annual solar calendar November;
2), screening
After seed ginseng is adopted and is caught, carry out the germplasm quality testing, filter out that the bodily form is cylindrical, cucumber shape, the back side swell slightly, has on the back side that vertical 4-6 capablely differs in size, the conical corn of irregular arrangement, the outside of belly is smooth, sucker is intensive, be arranged in irregular 3 vertical bands, the above individualities of body weight 250g;
3), transportation
Use the wet fortune of canvas bucket, traffic density is no more than 300/m 3
4), the cultivation of seed ginseng
(1), the density of seed ginseng in the pond is controlled at 20-30 head/m 3
(2), the intensification accelerating of seed ginseng is cultivated, be that solar calendar is gone into the pond mid-January, water temperature was controlled at 2-3 ℃ after kind participated in the pond, kept 2-3 days, after this heated up every day 1 ℃, when being raised to 6 ℃, constant temperature was cultivated 6-8 days, after this heated up every day 0.5 ℃, and constant temperature was cultivated 6-8 days when being raised to 10 ℃, after this heat up every day 0.5 ℃, constant temperature is cultivated and is waited to produce when being raised to 15 ℃;
(3), the throw something and feed mixture of powdery manufactured feed and ooze, the injected volume of this mixture accounts for the 3-8% of seed ginseng gross weight in the pond, the weight ratio of powdery manufactured feed and ooze is 1:(6-8), wherein the powdery manufactured feed comprises the batching of following percentage by weight: the sea-tangle dry powder 30-50% that comes unstuck, mouse tail algae dry powder 10-20%, sargassum dry powder 15-25%, hole sea lettuce dry powder 8-13%, fish meal 5-12%, dusty yeast 4-8%, B B-complex 1-3%, feed intake every night, the back of throwing something and feeding was changed water or flowing water in 1.5-2.5 hour;
(4), water quality management
Dissolved oxygen content in the water of control pond is 5-8ml/L, and change water 1-3 every day, and each quantity of exchanged water is the 40-60% of Chi Zhongshui, cultivate the purpose of ight soil, residual bait and rubbish in the pond to reach thorough removing, inhale end 1-2 time every day, fell the pond once in every 3-5 days, seed ginseng is laid eggs and was stopped down the pond in preceding 7 days; The not bait throwing in preceding 1 week of laying eggs;
(5), patrol the pond
Extremely swell if find seed ginseng, tentacle and sucker are lax, turn on the health, curl, stiff or part is individual when dirty phenomenon occurring arranging, illustrates that the dissolved oxygen content in the water of pond is on the low side, need change water or inflation immediately;
(6), the sign of laying eggs
Fishing for after seed ginseng enters the workshop, it has when laying eggs on a small quantity focused stimulation more by the time.
2. according to the breeding method of the described a kind of stichopus japonicus seed ginseng of claim 1, it is characterized in that describedly adopting that to catch be to be adopted by diver diving to catch, adopt that used instrument does not have oil stain in the process of catching, it is pure and fresh to keep supporting temporarily on the ship trough inner water matter, in time change water, adopt the stichopus japonicus of catching and avoid strong illumination.
3. according to the breeding method of the described a kind of stichopus japonicus seed ginseng of claim 1, it is characterized in that the described depth of water is the sea area at 5-10m place, require substrate to be husky mud and gravel at the bottom of, the good habitat that marine algae resources such as Zostera marina, mouse tail algae, sargassum are abundant.
4. according to the breeding method of the described a kind of stichopus japonicus seed ginseng of claim 1, it is characterized in that described B B-complex be vitamin B1, B2, B6, B12, stablize among VC, nicotinic acid, folic acid, calcium pantothenate, inositol, Choline Chloride and vitamin A, D3, E, the K3 any two or more.
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Cited By (13)

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CN102524143A (en) * 2012-03-09 2012-07-04 山东省海洋水产研究所 Long-distance transportation method of large-scale stichopus japonicus seedlings
CN102812917A (en) * 2012-09-06 2012-12-12 烟台市水产研究所 Long-distance transport method for apostichopus japonicus
CN103004658A (en) * 2012-12-17 2013-04-03 江苏海辰科技集团有限公司 Stichopus japonicus-growing method
CN103181354A (en) * 2013-04-15 2013-07-03 蓬莱市天保海水养殖有限公司 Winter and summer temporary breeding method of stichopus japonicus using geothermal water for protecting postlarvae
CN103355224A (en) * 2012-03-27 2013-10-23 响水海辰农业发展有限公司 Novel stichopus japonicus fry breeding method
CN103461224A (en) * 2013-07-29 2013-12-25 山东安源水产股份有限公司 Selection method of breeding stichopus japonicus in stichopus japonicus cross breeding
CN103750034A (en) * 2014-01-06 2014-04-30 大连海洋大学 Sea cucumber feed and method for pond culture of sea cucumber by utilizing sea cucumber feed
CN104108796A (en) * 2014-07-29 2014-10-22 大连海洋大学 Method for preventing and controlling generation of cladophora and/or enteromorpha in stichopus japonicas aquaculture pond by virtue of ulva
CN104521823A (en) * 2014-12-09 2015-04-22 青岛农业大学 Method for artificial ripening of California red ginseng
CN106259121A (en) * 2016-08-25 2017-01-04 大连棒棰岛海参发展有限公司 The method utilizing Radix Morinae Bulleyanae nursery pond waste water accelerating Radix Morinae Bulleyanae parent participation in winter
CN107549515A (en) * 2017-10-23 2018-01-09 福建天马科技集团股份有限公司 A kind of stichopus japonicus into ginseng stage mixed feed
CN112997934A (en) * 2021-03-18 2021-06-22 大连海洋大学 Method for promoting reproductive capacity of parent stichopus japonicus
CN115413612A (en) * 2022-09-15 2022-12-02 中国科学院南海海洋研究所 Non-irritant gathering and spawning method for holothuria leucospilota

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CN102084833A (en) * 2009-12-04 2011-06-08 盘锦光合水产有限公司 Ecological culture method for sea cucumber seedling in outdoor net cage
CN102100195A (en) * 2010-12-20 2011-06-22 山东海益宝水产股份有限公司 Method for breeding apostichopus japonicus selenka

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CN101595847A (en) * 2009-07-01 2009-12-09 大连水产学院 The method of inducing Stichopus Japonicus Selenka to lay eggs in vivo
CN101692796A (en) * 2009-10-21 2010-04-14 大连北方海洋生物发展有限公司 Natural sea area large-scale ecological breeding method of stichopus japonicus
CN102084833A (en) * 2009-12-04 2011-06-08 盘锦光合水产有限公司 Ecological culture method for sea cucumber seedling in outdoor net cage
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Publication number Priority date Publication date Assignee Title
CN102524143A (en) * 2012-03-09 2012-07-04 山东省海洋水产研究所 Long-distance transportation method of large-scale stichopus japonicus seedlings
CN103355224B (en) * 2012-03-27 2016-05-04 响水海辰农业发展有限公司 A kind of mating system of novel juvenile stichopus
CN103355224A (en) * 2012-03-27 2013-10-23 响水海辰农业发展有限公司 Novel stichopus japonicus fry breeding method
CN102812917A (en) * 2012-09-06 2012-12-12 烟台市水产研究所 Long-distance transport method for apostichopus japonicus
CN103004658A (en) * 2012-12-17 2013-04-03 江苏海辰科技集团有限公司 Stichopus japonicus-growing method
CN103181354A (en) * 2013-04-15 2013-07-03 蓬莱市天保海水养殖有限公司 Winter and summer temporary breeding method of stichopus japonicus using geothermal water for protecting postlarvae
CN103181354B (en) * 2013-04-15 2015-04-01 蓬莱市天保海水养殖有限公司 Winter and summer temporary breeding method of stichopus japonicus using geothermal water for protecting postlarvae
CN103461224B (en) * 2013-07-29 2015-10-21 山东安源水产股份有限公司 The system of selection of seed ginseng in stichopus japonicus crossbreeding
CN103461224A (en) * 2013-07-29 2013-12-25 山东安源水产股份有限公司 Selection method of breeding stichopus japonicus in stichopus japonicus cross breeding
CN103750034A (en) * 2014-01-06 2014-04-30 大连海洋大学 Sea cucumber feed and method for pond culture of sea cucumber by utilizing sea cucumber feed
CN104108796B (en) * 2014-07-29 2016-01-20 大连海洋大学 The method of bristle algae and/or Enteromorpha is broken out with sea lettuce prevention and control apostichopus japonicus culture pond
CN104108796A (en) * 2014-07-29 2014-10-22 大连海洋大学 Method for preventing and controlling generation of cladophora and/or enteromorpha in stichopus japonicas aquaculture pond by virtue of ulva
CN104521823A (en) * 2014-12-09 2015-04-22 青岛农业大学 Method for artificial ripening of California red ginseng
CN106259121A (en) * 2016-08-25 2017-01-04 大连棒棰岛海参发展有限公司 The method utilizing Radix Morinae Bulleyanae nursery pond waste water accelerating Radix Morinae Bulleyanae parent participation in winter
CN106259121B (en) * 2016-08-25 2019-01-18 大连棒棰岛海参发展有限公司 Utilize the method for winter stichopus japonicus nursery pond waste water accelerating stichopus japonicus parent participation
CN107549515A (en) * 2017-10-23 2018-01-09 福建天马科技集团股份有限公司 A kind of stichopus japonicus into ginseng stage mixed feed
CN112997934A (en) * 2021-03-18 2021-06-22 大连海洋大学 Method for promoting reproductive capacity of parent stichopus japonicus
CN115413612A (en) * 2022-09-15 2022-12-02 中国科学院南海海洋研究所 Non-irritant gathering and spawning method for holothuria leucospilota

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