CN102242184A - 一种四联菌片的菌种的活菌数检测方法 - Google Patents
一种四联菌片的菌种的活菌数检测方法 Download PDFInfo
- Publication number
- CN102242184A CN102242184A CN2011101613540A CN201110161354A CN102242184A CN 102242184 A CN102242184 A CN 102242184A CN 2011101613540 A CN2011101613540 A CN 2011101613540A CN 201110161354 A CN201110161354 A CN 201110161354A CN 102242184 A CN102242184 A CN 102242184A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- culture medium
- screening culture
- substratum
- tetrad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 241000186000 Bifidobacterium Species 0.000 title abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 31
- 238000012216 screening Methods 0.000 claims abstract description 30
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 230000003115 biocidal effect Effects 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims description 131
- 239000000843 powder Substances 0.000 claims description 44
- 241000194017 Streptococcus Species 0.000 claims description 42
- 230000001580 bacterial effect Effects 0.000 claims description 39
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000001888 Peptone Substances 0.000 claims description 14
- 108010080698 Peptones Proteins 0.000 claims description 14
- 235000019319 peptone Nutrition 0.000 claims description 14
- 229920001817 Agar Polymers 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 13
- 239000008272 agar Substances 0.000 claims description 13
- 235000015278 beef Nutrition 0.000 claims description 13
- 210000000941 bile Anatomy 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 11
- 229920000053 polysorbate 80 Polymers 0.000 claims description 11
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 9
- 239000008101 lactose Substances 0.000 claims description 9
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- 229910019142 PO4 Inorganic materials 0.000 claims description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 7
- 239000000306 component Substances 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 7
- 239000010452 phosphate Substances 0.000 claims description 7
- 229910052700 potassium Inorganic materials 0.000 claims description 7
- 239000011591 potassium Substances 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- RDEIXVOBVLKYNT-HDZPSJEVSA-N (2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-[(1r)-1-aminoethyl]oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(aminomethyl)oxan-2 Chemical compound OS(O)(=O)=O.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@@H](CN)O2)N)[C@@H](N)C[C@H]1N.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@H](O2)[C@@H](C)N)N)[C@@H](N)C[C@H]1N.O1[C@H]([C@@H](C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N RDEIXVOBVLKYNT-HDZPSJEVSA-N 0.000 claims description 6
- 241000283690 Bos taurus Species 0.000 claims description 6
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 4
- 239000001393 triammonium citrate Substances 0.000 claims description 4
- 235000011046 triammonium citrate Nutrition 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 235000021336 beef liver Nutrition 0.000 claims description 3
- 230000002779 inactivation Effects 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 2
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 claims description 2
- 239000006041 probiotic Substances 0.000 abstract description 14
- 235000018291 probiotics Nutrition 0.000 abstract description 14
- 240000001046 Lactobacillus acidophilus Species 0.000 abstract description 4
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 abstract description 4
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract description 4
- 244000199866 Lactobacillus casei Species 0.000 abstract 3
- 235000013958 Lactobacillus casei Nutrition 0.000 abstract 3
- 241000194020 Streptococcus thermophilus Species 0.000 abstract 3
- 229940017800 lactobacillus casei Drugs 0.000 abstract 3
- 241001608472 Bifidobacterium longum Species 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940009291 bifidobacterium longum Drugs 0.000 abstract 1
- 235000013402 health food Nutrition 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 31
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 22
- 238000004321 preservation Methods 0.000 description 15
- 239000000203 mixture Substances 0.000 description 14
- 239000000523 sample Substances 0.000 description 13
- 230000001954 sterilising effect Effects 0.000 description 13
- 230000009849 deactivation Effects 0.000 description 12
- 230000000529 probiotic effect Effects 0.000 description 12
- 238000000926 separation method Methods 0.000 description 12
- 239000012530 fluid Substances 0.000 description 11
- 235000014655 lactic acid Nutrition 0.000 description 11
- 238000004659 sterilization and disinfection Methods 0.000 description 11
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 10
- 238000004108 freeze drying Methods 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- 230000001681 protective effect Effects 0.000 description 9
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 230000002496 gastric effect Effects 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 239000002994 raw material Substances 0.000 description 8
- 238000003805 vibration mixing Methods 0.000 description 8
- 238000011081 inoculation Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 235000013336 milk Nutrition 0.000 description 7
- 239000008267 milk Substances 0.000 description 7
- 210000004080 milk Anatomy 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000002253 acid Substances 0.000 description 6
- 229940099352 cholate Drugs 0.000 description 6
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 6
- 239000003085 diluting agent Substances 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 238000012797 qualification Methods 0.000 description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical group [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- 238000000386 microscopy Methods 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 229910052708 sodium Inorganic materials 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 235000021262 sour milk Nutrition 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 241000237502 Ostreidae Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 235000020636 oyster Nutrition 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 108010009004 proteose-peptone Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000452 restraining effect Effects 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 241001037822 Bacillus bacterium Species 0.000 description 3
- 241001478240 Coccus Species 0.000 description 3
- 238000003794 Gram staining Methods 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- 235000013365 dairy product Nutrition 0.000 description 3
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 235000013923 monosodium glutamate Nutrition 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 239000006916 nutrient agar Substances 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000035479 physiological effects, processes and functions Effects 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 229960004249 sodium acetate Drugs 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- 229940073490 sodium glutamate Drugs 0.000 description 3
- 238000009777 vacuum freeze-drying Methods 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 description 2
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- -1 citric acid diamines Chemical class 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000006160 differential media Substances 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 235000019800 disodium phosphate Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 235000015138 kumis Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000002797 proteolythic effect Effects 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000321422 Mycteroperca jordani Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229960002227 clindamycin Drugs 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 235000013569 fruit product Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Inorganic materials [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000003375 selectivity assay Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000004206 stomach function Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201110161354 CN102242184B (zh) | 2011-06-16 | 2011-06-16 | 一种四联菌片的菌种的活菌数检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201110161354 CN102242184B (zh) | 2011-06-16 | 2011-06-16 | 一种四联菌片的菌种的活菌数检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102242184A true CN102242184A (zh) | 2011-11-16 |
CN102242184B CN102242184B (zh) | 2013-07-03 |
Family
ID=44960429
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201110161354 Expired - Fee Related CN102242184B (zh) | 2011-06-16 | 2011-06-16 | 一种四联菌片的菌种的活菌数检测方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102242184B (zh) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105296591A (zh) * | 2015-11-26 | 2016-02-03 | 佛山市海天(高明)调味食品有限公司 | 一种用于检测食品中难培养型乳酸菌的培养基以及检测方法 |
CN106191200A (zh) * | 2016-07-18 | 2016-12-07 | 内蒙古农业大学 | 一种选择性计数酸奶中保加利亚杆菌的方法 |
CN106319024A (zh) * | 2016-11-18 | 2017-01-11 | 合生元(广州)健康产品有限公司 | 一种包埋型益生菌产品的活菌数量检测方法 |
CN106434843A (zh) * | 2016-10-27 | 2017-02-22 | 江南大学 | 一种选择性分离培养b群链球菌的培养基及其制备方法 |
CN107287275A (zh) * | 2016-03-31 | 2017-10-24 | 启新生物科技有限公司 | 培养基、包含其的试剂盒、及其应用 |
CN109541205A (zh) * | 2018-12-07 | 2019-03-29 | 河南省商业科学研究所有限责任公司 | 一种益生菌中乳酸菌的检测方法 |
CN110699468A (zh) * | 2019-10-29 | 2020-01-17 | 圣湘生物科技股份有限公司 | 检测人肠道细菌的组合物、试剂盒及方法 |
CN112813135A (zh) * | 2021-04-07 | 2021-05-18 | 河北一然生物科技有限公司 | 一种植物乳杆菌中混入干酪类菌株的检测方法 |
CN112813134A (zh) * | 2021-04-07 | 2021-05-18 | 河北一然生物科技有限公司 | 显色培养基、制备方法及菌株检测方法 |
CN117051074A (zh) * | 2023-10-12 | 2023-11-14 | 山东省食品药品检验研究院 | 一种基于代谢通路分析的四联活菌制品活菌计数用培养基、计数方法及培养基的筛选方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006125446A2 (en) * | 2005-05-27 | 2006-11-30 | Chr. Hansen A/S | Quantification of the viability of lactic acid bacteria using flow cytometry |
CN1940085A (zh) * | 2006-09-28 | 2007-04-04 | 东北林业大学 | 双歧杆菌等严格厌氧菌的双层平板计数法 |
JP2007515153A (ja) * | 2003-08-29 | 2007-06-14 | ニュートリション フィジオロジィ コーポレイション | 特定微生物の検出及び定量方法 |
CN101319250A (zh) * | 2007-06-06 | 2008-12-10 | 东北农业大学 | 快速定量检测活的双歧杆菌的方法 |
-
2011
- 2011-06-16 CN CN 201110161354 patent/CN102242184B/zh not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007515153A (ja) * | 2003-08-29 | 2007-06-14 | ニュートリション フィジオロジィ コーポレイション | 特定微生物の検出及び定量方法 |
WO2006125446A2 (en) * | 2005-05-27 | 2006-11-30 | Chr. Hansen A/S | Quantification of the viability of lactic acid bacteria using flow cytometry |
CN1940085A (zh) * | 2006-09-28 | 2007-04-04 | 东北林业大学 | 双歧杆菌等严格厌氧菌的双层平板计数法 |
CN101319250A (zh) * | 2007-06-06 | 2008-12-10 | 东北农业大学 | 快速定量检测活的双歧杆菌的方法 |
Non-Patent Citations (6)
Title |
---|
《Immunology and Cell Biology》 20001231 KAILA KAILASAPATHY,et al. "Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp." 第80-88页 1-9 第78卷, * |
《生物医学工程学进展》 20101231 陈国霞 等 "双歧杆菌四联活菌片中嗜酸乳杆菌的分离及生长动力学研究" 第76-80页 1-9 第31卷, 第2期 * |
《食品科学》 20081231 辛若竹 等 "活性酸乳中每种益生菌总数的同时计数法" 第244-248页 1-9 第29卷, 第4期 * |
KAILA KAILASAPATHY,ET AL.: ""Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp."", 《IMMUNOLOGY AND CELL BIOLOGY》 * |
辛若竹 等: ""活性酸乳中每种益生菌总数的同时计数法"", 《食品科学》 * |
陈国霞 等: ""双歧杆菌四联活菌片中嗜酸乳杆菌的分离及生长动力学研究"", 《生物医学工程学进展》 * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105296591B (zh) * | 2015-11-26 | 2018-08-07 | 佛山市海天(高明)调味食品有限公司 | 一种用于检测食品中难培养型乳酸菌的培养基以及检测方法 |
CN105296591A (zh) * | 2015-11-26 | 2016-02-03 | 佛山市海天(高明)调味食品有限公司 | 一种用于检测食品中难培养型乳酸菌的培养基以及检测方法 |
CN107287275B (zh) * | 2016-03-31 | 2022-02-01 | 启新生物科技有限公司 | 培养基、包含其的试剂盒、及其应用 |
CN107287275A (zh) * | 2016-03-31 | 2017-10-24 | 启新生物科技有限公司 | 培养基、包含其的试剂盒、及其应用 |
CN106191200A (zh) * | 2016-07-18 | 2016-12-07 | 内蒙古农业大学 | 一种选择性计数酸奶中保加利亚杆菌的方法 |
CN106191200B (zh) * | 2016-07-18 | 2019-05-07 | 内蒙古农业大学 | 一种选择性计数酸奶中保加利亚杆菌的方法 |
CN106434843A (zh) * | 2016-10-27 | 2017-02-22 | 江南大学 | 一种选择性分离培养b群链球菌的培养基及其制备方法 |
CN106319024A (zh) * | 2016-11-18 | 2017-01-11 | 合生元(广州)健康产品有限公司 | 一种包埋型益生菌产品的活菌数量检测方法 |
CN109541205A (zh) * | 2018-12-07 | 2019-03-29 | 河南省商业科学研究所有限责任公司 | 一种益生菌中乳酸菌的检测方法 |
CN109541205B (zh) * | 2018-12-07 | 2022-01-28 | 河南省商业科学研究所有限责任公司 | 一种益生菌中乳酸菌的检测方法 |
CN110699468A (zh) * | 2019-10-29 | 2020-01-17 | 圣湘生物科技股份有限公司 | 检测人肠道细菌的组合物、试剂盒及方法 |
CN110699468B (zh) * | 2019-10-29 | 2023-05-02 | 圣湘生物科技股份有限公司 | 检测人肠道细菌的组合物、试剂盒及方法 |
CN112813135A (zh) * | 2021-04-07 | 2021-05-18 | 河北一然生物科技有限公司 | 一种植物乳杆菌中混入干酪类菌株的检测方法 |
CN112813134A (zh) * | 2021-04-07 | 2021-05-18 | 河北一然生物科技有限公司 | 显色培养基、制备方法及菌株检测方法 |
CN112813134B (zh) * | 2021-04-07 | 2022-04-26 | 河北一然生物科技股份有限公司 | 显色培养基、制备方法及菌株检测方法 |
CN117051074A (zh) * | 2023-10-12 | 2023-11-14 | 山东省食品药品检验研究院 | 一种基于代谢通路分析的四联活菌制品活菌计数用培养基、计数方法及培养基的筛选方法 |
CN117051074B (zh) * | 2023-10-12 | 2023-12-19 | 山东省食品药品检验研究院 | 一种基于代谢通路分析的四联活菌制品活菌计数用培养基、计数方法及培养基的筛选方法 |
Also Published As
Publication number | Publication date |
---|---|
CN102242184B (zh) | 2013-07-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102242184B (zh) | 一种四联菌片的菌种的活菌数检测方法 | |
CN101338283B (zh) | 一种干酪乳杆菌及其在固态发酵中的应用 | |
CN101333505B (zh) | 一种干酪乳杆菌在抗氧化作用方面的应用 | |
CN102204679B (zh) | 一种四联菌片及其制备方法 | |
CN104651268B (zh) | 一种植物乳杆菌及其应用 | |
CN105132318A (zh) | 植物乳杆菌Grx16及其应用 | |
CN110106119B (zh) | 一株分离自母乳的鼠李糖乳杆菌m9及其应用 | |
CN105368738A (zh) | 一种副干酪乳杆菌及其应用 | |
CN102604833B (zh) | 一种发酵乳多联发酵剂的制备方法及其产品 | |
CN108373984A (zh) | 一种副干酪乳杆菌及其应用 | |
CN104928208B (zh) | 一种植物乳杆菌Lp90及其筛选方法和应用 | |
CN113061543B (zh) | 一种植物乳杆菌及其用途 | |
CN105018379A (zh) | 一株具高抗氧化活性的植物乳杆菌及其应用 | |
CN101139557A (zh) | 一种干酪乳杆菌及其在改善血脂代谢和免疫调节中的应用 | |
CN105062918A (zh) | 一株植物乳杆菌及其应用 | |
CN107287133A (zh) | 一株植物乳杆菌lly‑606及其应用 | |
CN106883995A (zh) | 乳酸片球菌jqii-5菌株及其用途 | |
CN105420150A (zh) | 一种嗜酸乳杆菌及其应用 | |
CN103710290B (zh) | 源于广西巴马长寿村的益生菌鼠李糖乳杆菌hsryfm1301及其应用 | |
CN103952336B (zh) | 地衣芽孢杆菌、枯草芽孢杆菌和干酪乳杆菌制剂及制备 | |
CN104498401A (zh) | 一种动物双歧杆菌及其组合物 | |
CN103343107A (zh) | 一株人源具有抗氧化功能干酪乳杆菌grx12及其应用 | |
CN106635916A (zh) | 东方醋酸菌yzd‑09及其应用 | |
CN100424181C (zh) | 乳制品中双歧杆菌检测方法 | |
CN104450586B (zh) | 一种干酪乳杆菌及其组合物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: INNER MONGOLIA PUZE BIOLOGICAL PRODUCTS CO., LTD. Free format text: FORMER OWNER: GAO JIE Effective date: 20130128 Owner name: THE LIGHT INDUSTRY RESEARCH INSTITUTE OF INNER MON Effective date: 20130128 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20130128 Address after: 010080 the Inner Mongolia Autonomous Region Hohhot City Industrial Park, Jinchuan Development Zone West Gold seven road and Fenglin Road Interchange Applicant after: Neimenggu Puze Biological Product Co., Ltd. Applicant after: Neimenggu Light Industry Scientific Institute Co., Ltd. Applicant after: Inner Mongolia Beixing Biotechnology Co., Ltd. Address before: 010080 the Inner Mongolia Autonomous Region Hohhot City Industrial Park, Jinchuan Development Zone West Gold seven road and Fenglin Road Interchange Project of Inner Mongolia Biological Products Co. Ltd. Applicant before: Gao Jie |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130703 Termination date: 20170616 |