CN102220313A - 适用于作物ssr分子标记分析的基因组dna提取方法 - Google Patents
适用于作物ssr分子标记分析的基因组dna提取方法 Download PDFInfo
- Publication number
- CN102220313A CN102220313A CN2011101445441A CN201110144544A CN102220313A CN 102220313 A CN102220313 A CN 102220313A CN 2011101445441 A CN2011101445441 A CN 2011101445441A CN 201110144544 A CN201110144544 A CN 201110144544A CN 102220313 A CN102220313 A CN 102220313A
- Authority
- CN
- China
- Prior art keywords
- dna extraction
- gene group
- extracting method
- crop
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 41
- 238000007400 DNA extraction Methods 0.000 title claims abstract description 20
- 238000004458 analytical method Methods 0.000 title claims abstract description 12
- 239000003147 molecular marker Substances 0.000 title claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 title abstract description 10
- 108010067770 Endopeptidase K Proteins 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 11
- 244000105624 Arachis hypogaea Species 0.000 claims description 11
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 11
- 235000018262 Arachis monticola Nutrition 0.000 claims description 11
- 235000007164 Oryza sativa Nutrition 0.000 claims description 11
- 240000000111 Saccharum officinarum Species 0.000 claims description 11
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 11
- 240000008042 Zea mays Species 0.000 claims description 11
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 11
- 235000005822 corn Nutrition 0.000 claims description 11
- 235000020232 peanut Nutrition 0.000 claims description 11
- 235000009566 rice Nutrition 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 238000013016 damping Methods 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 10
- 238000012408 PCR amplification Methods 0.000 claims description 8
- 238000003805 vibration mixing Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 102000006382 Ribonucleases Human genes 0.000 abstract 1
- 108010083644 Ribonucleases Proteins 0.000 abstract 1
- 239000011536 extraction buffer Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 235000019082 Osmanthus Nutrition 0.000 description 24
- 241000333181 Osmanthus Species 0.000 description 24
- 244000242564 Osmanthus fragrans Species 0.000 description 12
- 235000019083 Osmanthus fragrans Nutrition 0.000 description 12
- 241000209094 Oryza Species 0.000 description 10
- 230000003321 amplification Effects 0.000 description 10
- 238000003199 nucleic acid amplification method Methods 0.000 description 10
- 239000003550 marker Substances 0.000 description 7
- 244000046052 Phaseolus vulgaris Species 0.000 description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 238000009395 breeding Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 101150095418 crop gene Proteins 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 206010019133 Hangover Diseases 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 238000001426 native polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2011101445441A CN102220313B (zh) | 2011-05-31 | 2011-05-31 | 适用于作物ssr分子标记分析的基因组dna提取方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2011101445441A CN102220313B (zh) | 2011-05-31 | 2011-05-31 | 适用于作物ssr分子标记分析的基因组dna提取方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102220313A true CN102220313A (zh) | 2011-10-19 |
CN102220313B CN102220313B (zh) | 2012-11-21 |
Family
ID=44777017
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2011101445441A Expired - Fee Related CN102220313B (zh) | 2011-05-31 | 2011-05-31 | 适用于作物ssr分子标记分析的基因组dna提取方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102220313B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103911435A (zh) * | 2014-03-13 | 2014-07-09 | 南宁泰格瑞农业科技有限公司 | 利用ssr标记与毛细管电泳鉴定甘蔗杂交种子纯度的方法 |
CN104017859A (zh) * | 2014-03-13 | 2014-09-03 | 南宁泰格瑞农业科技有限公司 | 一种基于ssr与毛细管电泳技术鉴定甘蔗种质资源的方法 |
CN105695446A (zh) * | 2016-02-24 | 2016-06-22 | 镇江华大检测有限公司 | 一种提取大豆种子全基因组dna的方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101294221A (zh) * | 2008-06-23 | 2008-10-29 | 福建农林大学 | 一种大批量快速制备pcr模板的方法 |
CN101413018A (zh) * | 2008-12-09 | 2009-04-22 | 中南大学 | 一种基因组dna的提取方法 |
CN101724696A (zh) * | 2009-10-28 | 2010-06-09 | 吉林大学 | 双温多重pcr鉴定猪早期胚胎性别的方法及试剂盒 |
-
2011
- 2011-05-31 CN CN2011101445441A patent/CN102220313B/zh not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101294221A (zh) * | 2008-06-23 | 2008-10-29 | 福建农林大学 | 一种大批量快速制备pcr模板的方法 |
CN101413018A (zh) * | 2008-12-09 | 2009-04-22 | 中南大学 | 一种基因组dna的提取方法 |
CN101724696A (zh) * | 2009-10-28 | 2010-06-09 | 吉林大学 | 双温多重pcr鉴定猪早期胚胎性别的方法及试剂盒 |
Non-Patent Citations (2)
Title |
---|
《天津农学院学报》 20070930 孙林静 等 一种简单快速的DNA提取方法在水稻上的应用 1-4 1-7 第14卷, 第3期 * |
李文强 等: "小麦线粒体DNA的高效提取方法", 《遗传 HEREDITAS》, vol. 29, no. 6, 30 June 2007 (2007-06-30), pages 771 - 775 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103911435A (zh) * | 2014-03-13 | 2014-07-09 | 南宁泰格瑞农业科技有限公司 | 利用ssr标记与毛细管电泳鉴定甘蔗杂交种子纯度的方法 |
CN104017859A (zh) * | 2014-03-13 | 2014-09-03 | 南宁泰格瑞农业科技有限公司 | 一种基于ssr与毛细管电泳技术鉴定甘蔗种质资源的方法 |
CN105695446A (zh) * | 2016-02-24 | 2016-06-22 | 镇江华大检测有限公司 | 一种提取大豆种子全基因组dna的方法 |
CN105695446B (zh) * | 2016-02-24 | 2019-01-11 | 镇江华大检测有限公司 | 一种提取大豆种子全基因组dna的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN102220313B (zh) | 2012-11-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101824484B (zh) | 一种用于棉花品种真实性鉴定的dna指纹检测方法 | |
US11926869B2 (en) | Development of simple sequence repeat (SSR) core primer group based on whole genome sequence of pomegranate and application thereof | |
CN107142324B (zh) | 桑树est-ssr分子标记及其核心引物组和应用 | |
CN111979345A (zh) | 盐胁迫条件下小麦生物量和产量相关kasp标记的开发及应用 | |
CN103866036B (zh) | 一种鉴别水稻千粒重基因tgw6的分子标记方法 | |
CN107022634A (zh) | 一种鉴别水稻抽穗期基因qHD7.4的分子标记方法 | |
CN107447012B (zh) | 一种利用ssr分子标记鉴定栽培茄与野生茄种间杂交f1杂种的方法 | |
CN102220313B (zh) | 适用于作物ssr分子标记分析的基因组dna提取方法 | |
CN1281751C (zh) | 用于评估种子纯度的dna标记和应用dna序列评估种子纯度的方法 | |
Paulauskas et al. | Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars. | |
CN108642207B (zh) | 一种快速准确鉴定越橘属植物的检测方法 | |
CN104805179A (zh) | 一种与甘蓝型油菜粒重关联的分子标记及制备方法和应用 | |
CN107254542B (zh) | 西瓜肉色性状主效基因位点及其InDel分子标记和应用 | |
CN103275968A (zh) | 一种快速提取棉花单粒种子dna的方法 | |
CN105087550A (zh) | 一种快速、高通量提取植物基因组dna的方法及应用 | |
CN106676176B (zh) | 一种利用多重pcr对四倍体紫花苜蓿进行ssr分析的方法 | |
CN106676171B (zh) | 一种棉花多基因聚合育种的分子检测方法 | |
CN101748217A (zh) | 辅助转育川麦42高产位点的分子标记技术 | |
CN104017801A (zh) | 一种微量提取辣椒单粒种子dna的方法 | |
CN113215220A (zh) | 一种基于转录组测序的橄榄ssr分子标记开发方法 | |
CN102586302B (zh) | 转Bt基因棉花标准质粒及其应用 | |
CN1884583A (zh) | 获取用于鉴定“三系”杂交稻保持系性状的分子标记方法 | |
CN102586303B (zh) | 转Bt基因水稻标准质粒及其应用 | |
Ning et al. | Genetic diversity analysis of sedges (Carex spp.) in Shandong, China based on inter-simple sequence repeat | |
CN106987625B (zh) | 快速鉴定食用向日葵杂交种三瑞6号真实性和纯度的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: NANJING TAIGERUI AGRICULTURAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: GUANGXI CROP GENETIC IMPROVEMENT AND BIOTECHNOLOGY KEY LAB Effective date: 20131029 Free format text: FORMER OWNER: MINISTRY OF AGRICULTURE SUGARCANE QUALITY SUPERVISION INSPECTION AND TESTING CENTER (NANNING) Effective date: 20131029 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20131029 Address after: 530007, B-1-1305, room, dragon spirit B, 33 Garden Avenue, the Guangxi Zhuang Autonomous Region, China Patentee after: Nanjing Taigerui Agricultural Technology Co., Ltd. Address before: 530007 the Guangxi Zhuang Autonomous Region XiXiangTang Nanning University Road No. 172 Patentee before: Gaungxi Crop Hereditary Improve Biotechnology Key Open Laboratory Patentee before: Sugarcane quality supervision, inspection and testing center of Ministry of agriculture (Nanning) |
|
CP03 | Change of name, title or address |
Address after: 530007 the Guangxi Zhuang Autonomous Region Nanning hi tech Zone Binhe Road No. 28, China ASEAN enterprise headquarters base three phase comprehensive building, Nanning advertising industry park, room A 1001 Patentee after: Guangxi Thailand Technology Co., Ltd. Address before: 530007, B-1-1305, room, dragon spirit B, 33 Garden Avenue, the Guangxi Zhuang Autonomous Region, China Patentee before: Nanjing Taigerui Agricultural Technology Co., Ltd. |
|
CP03 | Change of name, title or address | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121121 Termination date: 20210531 |
|
CF01 | Termination of patent right due to non-payment of annual fee |