CN102178635B - Use of leech for cleaning skin, skin cleaning composition and preparation method thereof - Google Patents
Use of leech for cleaning skin, skin cleaning composition and preparation method thereof Download PDFInfo
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- CN102178635B CN102178635B CN201110106164A CN201110106164A CN102178635B CN 102178635 B CN102178635 B CN 102178635B CN 201110106164 A CN201110106164 A CN 201110106164A CN 201110106164 A CN201110106164 A CN 201110106164A CN 102178635 B CN102178635 B CN 102178635B
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- 238000004140 cleaning Methods 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 241000545744 Hirudinea Species 0.000 title abstract description 6
- 239000000284 extract Substances 0.000 claims abstract description 24
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 claims abstract description 24
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- Cosmetics (AREA)
Abstract
The invention, belonging to the field of cosmetics, relates to a new use of leech, specifically to the use of leech as main raw material for cleaning skin, a skin cleaning composition prepared based on the characteristic and a preparation method. According to the invention, microcirculation of skin is improved by means of the blood circulation-promoting and stasis-dissolving effect of leech extract, natural hirudin and recombinant hirudin in order to relax skin as well as thoroughly cleanse dirt, blackheads and whiteheads in pores and aged cuticles (commonly referred to as dead skin) on the surface of skin and the like, therefore, superior foundation is laid for basic skincare. Based upon the above new use, the leech extract, the natural hirudin and the recombinant hirudin can be prepared to cosmetic cleansing products, including face cleansing cream, face cleansing gel, scrubbing cream, cream for removing dead skin, body wash, makeup remover, perfumed soap, etc.
Description
Technical field
The invention belongs to cosmetic field, relate to the new application of Hirudo, specifically is to be that primary raw material is used for cleaning skin with the Hirudo, and utilizes the skin cleansing compositions and the method for preparing of this characteristic preparation.
Background technology
Hirudo is a Chinese medicine simply, and history is stated from Shennong's Herbal, and the successive dynasties book on Chinese herbal medicine all has record.The traditional Chinese medical science thinks that it has the function of removing blood stasis, eliminating blood stasis and inducing menstruation, always is used to treat lump in the abdomen, mass in the abdomen, blood stasis amenorrhea, traumatic injury.Chinese Pharmacopoeia version in 2000 is recorded the dry body into Hirudinidae animal whitmania WhitmanpigraWhitman, Hirudo HinudenipponicaWhitman or Folium Salicis Babylonicae Hirudo WhitmaniaacranulataWhitman.In the Hirudo there be main component: 1, directly act on the composition of blood coagulation system, comprise thrombin inhibitor, and the material of other inhibition blood coagulation, like hirudin (Hirudin), Hirudinaria manillensis plain (Bufrudin), forest trematodiasis plain (Haemadin) etc.2, other protease inhibitor and other active component are like Cellosolve (Hementerin), treating can gloomy (Decorsin) etc.3, other compositions: the Hirudo master contains protein; Contain 17 seed amino acids in addition; Comprise 8 seed amino acids of needed by human, also contain 14 kinds of elements such as Zn, Mn, Fe, Co, Cr, Se, Mo, Ni, kiss nutritional labelings such as trematodiasis element, heparin, antithrombotic element, aminoacid ingredient in addition.
At present, use extract or its main component hirudin of Hirudo in the existing more cosmetics.Extract like Hirudo can be used for speckle dispelling, anti-acne, skin moistening, skin care, whitens, prevents skin aging, strengthens the effect of skin elasticity.
In the existing report, Shang Weijian uses report and the application of Hirudo in cleaning skin separately.Skin is the first line of defence of human body natural's defense system, skin health, and defence capability is just strong.Sense light, happy and that pass through is clearly given in the healthy and beautiful beautiful enjoyment of skin ability administration of human.Want to let skin health and beauty. at first will keep the sanitation and hygiene of skin.Because horny layer is aging, sebaceous gland and sweat gland secretion, other endocrines and external dust etc. are mingled in together and attached on the skin, thereby constitute dirt.On the one hand these dirts can stop up sweat gland, hinder the eubolism of skin, and very easily oxidation by air and produce offending stink of sebum on the other hand impels the breeding of pathogen, finally causes dermopathic generation, quickens the aging of skin.These factors can influence the performance of skin normal physiological function even cause illness such as skin infection, acne.This shows that skin clean is very important.The main purpose of cleaning skin has: remove skin surface dirt, oils and fats and secretions; Keep sebaceous gland, sweat gland secretion unimpeded; Prevent the infection of antibacterial; Make skin loosen rest, give full play to its physiological function; Regulate the pH value of skin, recover normal PH; Form the sebum film of appropriateness, the opposing environmental stimuli.Clean skin also is to prepare for the nursing of advancing-go on foot of skin.Inventor's expectation utilizes Hirudo to be used for cleaning skin, comprises whole skin, and facial.
Summary of the invention
The technical problem that the present invention solved provides the new purposes of a kind of Hirudo, is used for cleaning skin.
The present invention utilizes Hirudo extract, natural hirudin and lepirudin 023 ludon to have the effect of blood circulation promoting and blood stasis dispelling; To increase skin microcirculation; The skin of releiving; Completely with the dirt in the pore, blackhead, hoary hair, and skin surface aging cutin layer (being commonly called as dead bark) etc. remove clean, for basic skin protection ready.
Utilize above-mentioned new purposes; Can the cosmetics that Hirudo extract, natural hirudin and lepirudin 023 ludon are processed there be facial milk cleanser, clean gel, scrubbing cream, Exfoliating Scrub cream, bath foam, makeup removing liquid, fancy soap etc., add suitable adjuvant according to the needs of dosage form separately.
The present invention utilizes Hirudo to be used for cleaning skin, and it is obvious to dispel dirt, blackhead, hoary hair, the effect of aging cutin layer, for the public provides a kind of brand-new selection.
The specific embodiment
The present invention utilizes Hirudo extract, natural hirudin and lepirudin 023 ludon to have the effect of blood circulation promoting and blood stasis dispelling; To increase skin microcirculation; The skin of releiving; Completely with the dirt in the pore, blackhead, hoary hair, and skin surface aging cutin layer (being commonly called as dead bark) etc. remove clean, for basic skin protection ready.
One, confirming of active component dose-effect relationship of the present invention:
On the independent use test person's skin of Hirudo extract, natural hirudin and lepirudin 023 ludon solution in different concentration, every day, sooner or later each used once, used for 8 weeks after, according to the valid density of active component in the cleaning effect screening product.
The Hirudo extract that the present invention adopted, natural hirudin and lepirudin 023 ludon are respectively:
1, the conventional method for preparing of Hirudo extract is: get all rubbing of fresh Hirudo and process the Hirudo slurry; Proportioning by volume: 1 part in Hirudo slurry: the batching of 0.01～0.1 part of concentrated hydrochloric acid, 1～10 part in acetone is extracted; get acetone solution, and residue reuse acetone extraction filters; merge acetone extract, dry getting final product.
2, the natural hirudin extraction process is: get fresh Hirudo saliva, add acetone, and cooling, it is centrifugal to get supernatant, and residue adds the trichloroacetic acid dissolving, column chromatography in the sampling:
(1) anion exchange chromatography: get an amount of DEAE-C52 adding distil water and make its expansion; Be washed with distilled water to neutrality after respectively soaking 30min with NaOH-NaCl mixed liquor, dilute hydrochloric acid solution and NaOH-NaCl mixed liquor successively by conventional method; Behind the dress post, with the citric acid-sodium citrate buffer balance of pH4.6, appearance is carried out gradient elution and is drawn elution curve with monitor on the above-mentioned trichloroacetic acid solution; The Fractional Collections eluent; To have vigor partly to concentrate in the bag filter and concentrate with glycerol, the two vena contracta methods of reuse are surveyed total protein content, the column chromatography below continuing;
(2) gel filtration chromatography is got an amount of Sephadex-G25 adding distil water immersion 4h, and with NaOH-NaCl mixed liquid dipping 0.5h, sucking filtration is removed alkali liquor and is washed with distilled water to neutrality by conventional method, and heated and boiled is removed bubble.The dress post with the buffer balance of pH7.4, is drawn elution curve with on the above-mentioned concentrated solution that the vigor eluent arranged kind, eluting and with monitor, and Fractional Collections has the vigor eluent; Reclaim solvent, get final product after the drying.
Or directly buy the commercially available prod.
3, (patent No.: 01105798.X), the 32nd to 35 of its molecule and the 53rd to 66 amino acids sequence are lepirudin 023 ludon: Arg32-Gly33-Asp34-Ser35; Gln53-Gly54-Asp55-Phe56-Glu57-Pro58-Ile59-Pro60-Glu61-As p62-Ala63-Tyr64-Asp65-Glu66.
Test result is seen table 1:
The no positive effect of (-) expression
(+) expression produces effect
(++) expression has positive effect
(+++) expression has very positive effect
The cleaning effect table of table 1 leech active composition
The above table explanation, adopt the difference on effect of the Hirudo medicine of variable concentrations to be: when concentration is lower than 5%, DeGrain; When concentration is 5% when above, cleaning effect is obvious; But when concentration is increased to 25% when above, the tester is reflected too oily phenomenon can occur.To sum up experimental result is finally selected 5-25% (%, concentration g/g).
Two, the preparation of skin cleansing compositions of the present invention:
Utilize Hirudo to can be used for the new purposes of cleaning skin, can be made into skin cleansing compositions, comprise whole skin Cleasing compositions and facial skin cleansing compositions.Wherein, the whole skin Cleasing compositions is bath foam or fancy soap; The skin of face Cleasing compositions is facial milk cleanser, clean gel, Exfoliating Scrub scrubbing cream, makeup removing liquid or clean facial soap, and to account for the percentage by weight of skin cleansing compositions be 5-25% for Hirudo extract or natural hirudin or lepirudin 023 ludon in the said skin cleansing compositions.。
Prepare skin cleansing compositions of the present invention, adopt active component and adjuvant commonly used to get final product according to the conventional method preparation, its key component adopts following percentage by weight:
The preparation instance is following:
Embodiment 1: Hirudo extract facial milk cleanser prescription and preparation method thereof
Method for making: add N-acyl glutamic acid sodium in the water, place a container; Then, add hirudin extract, glycerol, be heated to 60～65 ℃ of stirring and dissolving.In another container, add Jojoba oil, POE (15) oleyl alcohol ether, heating stirs.Respectively two liquid in containers are filtered, mix, the degassing after fully mixing, cooling promptly get.
Embodiment 2: the prescription of natural hirudin facial milk cleanser and preparation:
Method for making: white oil, lanonol, sorbitan monostearate are heated to 80 ℃ (components 1); Natural hirudin is dissolved to postcooling to 80 ℃ (component 2) in the water under 95 ℃; Then component 2 is added in the component 1, be cooled to 40 ℃ under stirring, stirring gets final product.
Embodiment 3: the prescription and the method for preparing of clean gel of lepirudin 023 ludon:
Method for making: Carbopol 934 resins are dispersed in the water, add lepirudin 023 ludon again, be heated to 70 ℃ at present.White oil, monostearate Polyethylene Glycol (600) ester and triisopropanolamine are mixed and heated to 70 ℃ make its consolute, add to then in the resin solution, carry out high degree of agitation simultaneously, to be mixed evenly after, be chilled to room temperature and promptly get.
Embodiment 4: the prescription of Hirudo extract scrubbing cream and method for preparing:
Method for making: mix acetylated lanolin, stearic acid and Arlacel-60 (mixture 1) earlier, remix and Hirudo extract, glycerol, water (mixture 2) are heated with stirring to 70 ℃ with two parts.Stir down and slowly mixture 2 is added in the mixture 1, after stirring, add the micropore frosting agent, stirring to room temperature gets final product.
Embodiment 5: the prescription of Hirudo extract bath foam and method for preparing:
Method for making: sodium benzoate is added water place container, stir and be heated to 50 ℃, add Oleum Cocois first soap.Even back to be mixed adds glycerol, Hirudo extract, regulates viscosity with sodium chloride during near 40 ℃ of temperature.
Embodiment 6: natural hirudin fancy soap prescription and method for preparing:
Method for making: with above-mentioned other each composition mix homogeneously post-heating to 140 ℃, the spray-dried soap powder of processing, moulded section promptly gets.
Three, the cleaning skin compliance test result of product of the present invention:
Application example 1 (facial cleansing): Hirudo facial milk cleanser or clean gel products compliance test result:
Be distributed to 9 different testers with making product in the instance 1～3; Divide 3 groups and use 3 kinds of different products respectively; Sooner or later each uses once for each person every day, and consumption is with used clean product is consistent at ordinary times, use a period of time continuously after; According to experimenter's subjective assessment, evaluation skin of face dirt, blackhead, hoary hair, aging cutin layer dispel effect.
Test result is seen table 2:
The no positive effect of (-) expression
(+) expression produces effect
(++) expression has positive effect
(+++) expression has very positive effect
Clean compliance test result of table 2 product
Experimental result shows that clean product of Hirudo extract compared with natural hirudin, clean product of lepirudin 023 ludon, and produce effects is slow, possibly be to cause because hirudin content is low.Generally speaking, three kinds of products use 4 week back skin dirts, blackhead, hoary hair, aging cutin layer to dispel effect all more obvious, explain that the clean skin product of Hirudo is respond well.
Application example 2 (facial cleansing): Hirudo scrubbing cream and Exfoliating Scrub cream product compliance test result:
Be distributed to 3 bit test personnel with making product in the instance 4, use once weekly, consumption is 1～2g; After using a period of time continuously; According to experimenter's subjective assessment, evaluation skin of face dirt, blackhead, hoary hair, aging cutin layer dispel effect, the result sees table 3:
Clean compliance test result of table 3 product
Show through experimental result; The Hirudo extract scrubbing cream after using for 2 weeks all; Skin dirt, hoary hair, aging cutin layer to dispel effect all more obvious, used for 4 weeks after, pore cleanser, aging cutin layer effect are more remarkable; After 6 weeks of service time, the effect of pore cleanser and aging cutin layer is very obvious.
Application example 3 (whole skin): Hirudo bath foam and fancy soap product compliance test result:
Be distributed to 6 bit test personnel with making product in the instance 5,6; Divide 2 groups to use 2 kinds of different products respectively, everyone uses weekly once, use amount with the tester at ordinary times use amount be as the criterion; After using a period of time continuously; According to experimenter's subjective assessment, evaluate the effect of dispeling of skin dirt, aging cutin layer, the result sees table 4:
The checking of table 4 whole skin cleaning effect
Show through experimental result, Hirudo extract bath foam, natural hirudin fancy soap after using for 4 weeks all, skin dirt, aging cutin layer to dispel effect all more obvious, used for 4～8 weeks, skin dirt aging cutin layer effect be highly significant.
To sum up, the present invention utilizes Hirudo to be used for cleaning skin, and it is obvious to dispel dirt, blackhead, hoary hair, the effect of aging cutin layer, for the public provides a kind of brand-new selection.
Claims (6)
1. Hirudo is used for the application of cleaning skin, it is characterized in that: the extract or natural hirudin or the lepirudin 023 ludon that adopt Hirudo.
2. Hirudo according to claim 1 is used for the application of cleaning skin, it is characterized in that: extract of employing Hirudo or natural hirudin or lepirudin 023 ludon are active component, add conventional adjuvant and process skin cleansing compositions.
3. Hirudo according to claim 2 is used for the application of cleaning skin, it is characterized in that: said skin cleansing compositions is whole skin Cleasing compositions or skin of face Cleasing compositions.
4. Hirudo according to claim 3 is used for the application of cleaning skin, it is characterized in that: the whole skin Cleasing compositions is bath foam or fancy soap; Said skin of face Cleasing compositions is facial milk cleanser, clean gel, Exfoliating Scrub scrubbing cream, makeup removing liquid or clean facial soap.
5. Hirudo according to claim 2 is used for the application of cleaning skin, it is characterized in that: to account for the percentage by weight of skin cleansing compositions be 5-25% for Hirudo extract or natural hirudin or lepirudin 023 ludon in the said skin cleansing compositions.
6. Hirudo according to claim 2 is used for the application of cleaning skin, it is characterized in that: said skin cleansing compositions is made up of the component of following weight percentage ratio:
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| CN103006512B (en) * | 2012-12-21 | 2014-06-11 | 山东侨牌集团有限公司 | Hirudin anti-winkle face-softening conditioning cream and production process thereof |
| CN105078802A (en) * | 2014-05-15 | 2015-11-25 | 钱如力 | Acidic polypeptide transdermal caring agent and method for preparing same |
| WO2016168179A1 (en) * | 2015-04-13 | 2016-10-20 | Capsulent | Succulent extract and alginate combined solutions and products incorporating them |
| CN105997559A (en) * | 2016-05-11 | 2016-10-12 | 南京泽丽生物技术有限公司 | Skin-beautifying and anti-pox composition and preparing method thereof |
| CN108175787A (en) * | 2018-01-30 | 2018-06-19 | 广州蛭肽素医药科技有限公司 | A kind of Chinese medicinal ointment for alleviating chloasma and preparation method thereof |
| CN109652239A (en) * | 2018-11-27 | 2019-04-19 | 安徽省玉兔日用化学品有限公司 | A kind of amino acid perfumed soap and its processing technology containing Rhizoma Polygonati extract |
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| CN1732897A (en) * | 2005-08-16 | 2006-02-15 | 周维海 | Face-beautifying skin-caring lotion with leech extract and its preparation process |
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| CN1732897A (en) * | 2005-08-16 | 2006-02-15 | 周维海 | Face-beautifying skin-caring lotion with leech extract and its preparation process |
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