CN102165945B - Rabbit semen diluent nutrition powder and preparation method thereof - Google Patents

Rabbit semen diluent nutrition powder and preparation method thereof Download PDF

Info

Publication number
CN102165945B
CN102165945B CN201110004364.3A CN201110004364A CN102165945B CN 102165945 B CN102165945 B CN 102165945B CN 201110004364 A CN201110004364 A CN 201110004364A CN 102165945 B CN102165945 B CN 102165945B
Authority
CN
China
Prior art keywords
rabbit
semen diluent
nutrition powder
rabbit semen
enrofloxacin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201110004364.3A
Other languages
Chinese (zh)
Other versions
CN102165945A (en
Inventor
淡瑞芳
张海容
张海涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Agri Animal Husbandry Vocational College
Original Assignee
Jiangsu Agri Animal Husbandry Vocational College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Agri Animal Husbandry Vocational College filed Critical Jiangsu Agri Animal Husbandry Vocational College
Priority to CN201110004364.3A priority Critical patent/CN102165945B/en
Publication of CN102165945A publication Critical patent/CN102165945A/en
Application granted granted Critical
Publication of CN102165945B publication Critical patent/CN102165945B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses rabbit semen diluent nutrition powder which is prepared from the following components: 2-4 g of tris(hydroxymethyl)aminomethane, 0.5-1.5 g of glucose, 1-3 g of citric acid, 3-8 mg of kanamycin and 3-8 mg of enrofloxacin. The invention also discloses a preparation method of the rabbit semen diluent nutrition powder, which comprises the following steps: (I) weighing raw materials: weighing 2-4 g of tris(hydroxymethyl)aminomethane, 0.5-1.5 g of glucose, 1-3 g of citric acid, 3-8 mg of kanamycin and 3-8 mg of enrofloxacin, and uniformly mixing the raw materials with a vortex mixer to obtain the rabbit semen diluent nutrition powder; and (II) packaging: vacuum-packaging the uniformly mixed rabbit semen diluent powder, and preventing pollution during the entire operation. Through the invention, enough nutrient substances are provided for sperms, the sperm motility maintaining time can be effectively prolonged, and the conception rate of female rabbits is improved.

Description

A kind of rabbit semen diluent nutrition powder and preparation method thereof
Technical field
The present invention relates to a kind of rabbit semen diluent nutrition powder and preparation method thereof.
Background technology
Along with the development of rabbit keeping, select the seminal fluid of good species buck, improve rabbit group's quality and reproduction rate, the development of rabbit industry is had to great meaning.Reaching its maturity of current various domestic animal technology of artificial insemination, level of application is increasingly universal, has had more than 20 year although this technology is applied in meat rabbit production, can't reach application widely, and one of them important factor is the preservation problem of seminal fluid.Liquid storage can solve the transportation problem of seminal fluid, extensively provides condition for artificial insemination generally pushes away use, can concentrate and carry out rabbit childbirth and management.
Summary of the invention
The invention provides a kind of rabbit semen diluent nutrition powder, it not only provides enough nutriments to sperm, carry out the buffering of acid or alkali environment, maintain suitable osmotic pressure, the growth of anti-bacteria, and by this rabbit semen diluent nutrition powder dilution seminal fluid, can effectively extend the holding time of sperm viability, improve the conception rate of female rabbit, simultaneously easy to use in production application.
The present invention has adopted following technical scheme: a kind of rabbit semen diluent nutrition powder, it comprises following composition: trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg are formulated.
The content of the each composition of the present invention is: trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.
The preparation method who the invention discloses a kind of rabbit semen diluent nutrition powder, it comprises the following steps:
The first step; Raw material takes: take trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg, above-mentioned raw materials is mixed with vortex mixer, obtain rabbit semen diluent nutrition powder; Second step; Packaging: the rabbit sperm dilution powder mixing is carried out to vacuum packaging, prevent from polluting in whole operating process.
Described first step trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.
The present invention has following beneficial effect: rabbit semen diluent nutrition powder prepared by the present invention, contain trishydroxymethylaminomethane, glucose, citric acid, kanamycin and Enrofloxacin, nutrient powder distilled water dissolved dilution, can carry out liquid storage to rabbit seminal fluid, implement the artificial insemination of rabbit, make to preserve the time lengthening 84-96 hour of rabbit seminal fluid, sperm viability is that 0.4 grade of above effective time-to-live is 48 ± 4, 6 hours, primipara rabbit conception rate is more than 90%, the female rabbit of multiparity is more than 75%, so not only provide enough nutriments to sperm, carry out the buffering of acid or alkali environment, maintain suitable osmotic pressure, the growth of anti-bacteria, and by this rabbit semen diluent nutrition powder dilution seminal fluid, can effectively extend the holding time of sperm viability, improve the conception rate of female rabbit, simultaneously easy to use in production application.
Embodiment
The invention discloses a kind of rabbit semen diluent nutrition powder, it comprises following composition: trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg are formulated.
Further illustrate this invention below by implementing one.
Embodiment mono-, the invention provides a kind of rabbit semen diluent nutrition powder, and it comprises following composition: trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.The present invention makes by the following method: the first step; Raw material takes: take trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg, above-mentioned raw materials is mixed with vortex mixer, obtain rabbit semen diluent nutrition powder; Second step; Packaging: the rabbit sperm dilution powder mixing is carried out to vacuum packaging, should make operation room without dust, ventilation, prevent from polluting rabbit semen diluent nutrition powder in whole operating process.
Rabbit sperm dilution powder of the present invention dissolves with 100ml distilled water, with gathered rabbit seminal fluid with 1: 6-10 doubly dilutes (according to seminal fluid density), preserve in 15 DEG C of constant temperature of room temperature, can make sperm prolonged survival period to 84-96 hour, sperm viability is that 0.4 grade of above effective time-to-live is 48 ± 4,6 hours.
Rabbit semen diluent nutrition powder of the present invention carries out the artificial insemination of Qingdao meat rabbit plant of Kang great food Co., Ltd in March, 2010-September, and by the artificial insemination of 6000 times, primipara rabbit conception rate is up to 93%, and the female rabbit conception rate of multiparity reaches 78%.

Claims (1)

1. a rabbit semen diluent nutrition powder, is characterized in that it comprises following composition: trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg are formulated, and its preparation method is as follows, and it comprises the following steps:
The first step; Raw material takes: take trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg formulated, above-mentioned raw materials is mixed with vortex mixer, obtain rabbit semen diluent nutrition powder;
Second step; Packaging: the rabbit sperm dilution powder mixing is carried out to vacuum packaging, prevent from polluting in whole operating process.
CN201110004364.3A 2011-01-11 2011-01-11 Rabbit semen diluent nutrition powder and preparation method thereof Expired - Fee Related CN102165945B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201110004364.3A CN102165945B (en) 2011-01-11 2011-01-11 Rabbit semen diluent nutrition powder and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201110004364.3A CN102165945B (en) 2011-01-11 2011-01-11 Rabbit semen diluent nutrition powder and preparation method thereof

Publications (2)

Publication Number Publication Date
CN102165945A CN102165945A (en) 2011-08-31
CN102165945B true CN102165945B (en) 2014-09-03

Family

ID=44487414

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201110004364.3A Expired - Fee Related CN102165945B (en) 2011-01-11 2011-01-11 Rabbit semen diluent nutrition powder and preparation method thereof

Country Status (1)

Country Link
CN (1) CN102165945B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112106764A (en) * 2020-09-25 2020-12-22 南京农业大学 Normal-temperature antioxidant rabbit preservation diluent and preparation and use methods thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1927226A (en) * 2006-09-11 2007-03-14 华中农业大学 Cow sex-control sperm freezing preservation seminal fluid dilution
CN101611705A (en) * 2009-07-23 2009-12-30 于文龙 A kind of diluting boar semen powder and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1927226A (en) * 2006-09-11 2007-03-14 华中农业大学 Cow sex-control sperm freezing preservation seminal fluid dilution
CN101611705A (en) * 2009-07-23 2009-12-30 于文龙 A kind of diluting boar semen powder and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"不同稀释液对猪精液常温保存效果的研究";茹达干等;《动物医学进展》;20020120;第23卷(第1期);第77页第3.2节 *
茹达干等."不同稀释液对猪精液常温保存效果的研究".《动物医学进展》.2002,第23卷(第1期),第77页第3.2节.

Also Published As

Publication number Publication date
CN102165945A (en) 2011-08-31

Similar Documents

Publication Publication Date Title
CN104671881A (en) Vegetable soilless culture nutrient solution and manufacturing method thereof
CN102627483B (en) Organic concentrated nutrient solution special for flowers and potted landscapes and preparation method thereof
CN107183014B (en) Efficient grouper sperm cryopreservation liquid and using method thereof
CN101849572B (en) Method for keeping bamboo shoots fresh
CN102369955A (en) Preparation for controlling vegetable insect disease
CN104446918A (en) Method for manufacturing soilless culture substrate by utilizing apple tree fruit drop
CN103708873A (en) Plant nutrient solution special for kaffir lily
CN102246745B (en) Antifreeze for cryopreservation of spermatogonial stem cells of animals and cryopreservation method thereof
CN105948950A (en) {0><}0{>Improved tree nutrient solution
CN104649810A (en) Method for preparing green nutritional soils of flowers
CN104261908B (en) A kind of biological organic nutrient liquid fertilizer
CN102424432A (en) Artificial seawater preparation as well as preparation method and use method thereof
CN102771472B (en) Freezing liquid for preserving embryo, preparation method and application thereof
CN102293338B (en) Artificial forage for larvae of Holotrichia parallcla, preparation method thereof, and application thereof
CN102165945B (en) Rabbit semen diluent nutrition powder and preparation method thereof
CN102816012A (en) Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium
CN110511091A (en) Soilless culture nutrient fluid and the soilless culture method of herbage
CN102503714B (en) Special fertilizer for transplanting tobacco
CN102726371A (en) Long-acting boar semen diluting powder and preparation method thereof
CN103103143B (en) Nitrifying bacteria preservation method
CN105037007A (en) Culture soil of prunus mume flowers and preparation method thereof
CN108892594A (en) A kind of plant nutrition liquid enhancing sprigging survival rate
CN104529640A (en) Leaf vegetable special fertilizer containing amino acid and preparing method thereof
CN104016788A (en) Water-soluble biogas manure and preparation method thereof
CN104211520A (en) Preparation method of pine needle matrix nutrition soil

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Free format text: FORMER OWNER: ZHANG HAIRONG ZHANG HAITAO

Effective date: 20140807

Owner name: JIANGSU AGRI-ANIMAL HUSBANDRY VOCATIONAL COLLEGE

Free format text: FORMER OWNER: RUIFANG DAN

Effective date: 20140807

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20140807

Address after: 225300 Taizhou Fenghuang Road, Jiangsu, No. 8

Applicant after: Jiangsu Agri-Animal Husbandry Vocational College

Address before: 225300 Taizhou Fenghuang Road, Jiangsu, No. 8

Applicant before: Dan Ruifang

Applicant before: Zhang Hairong

Applicant before: Zhang Haitao

C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140903

Termination date: 20190111

CF01 Termination of patent right due to non-payment of annual fee