CN102816012A - Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium - Google Patents
Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium Download PDFInfo
- Publication number
- CN102816012A CN102816012A CN2012103538267A CN201210353826A CN102816012A CN 102816012 A CN102816012 A CN 102816012A CN 2012103538267 A CN2012103538267 A CN 2012103538267A CN 201210353826 A CN201210353826 A CN 201210353826A CN 102816012 A CN102816012 A CN 102816012A
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- Prior art keywords
- culture medium
- hypsizygus marmoreus
- preservation
- lime powder
- wheat bran
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- 241001534815 Hypsizygus marmoreus Species 0.000 title claims abstract description 22
- 239000001963 growth medium Substances 0.000 title claims abstract description 17
- 238000004321 preservation Methods 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title abstract 4
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims abstract description 19
- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 19
- 239000004571 lime Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 19
- 239000002023 wood Substances 0.000 claims abstract description 19
- 229920001817 Agar Polymers 0.000 claims abstract description 10
- 239000008272 agar Substances 0.000 claims abstract description 10
- 239000002245 particle Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 9
- 230000003020 moisturizing effect Effects 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 241000233866 Fungi Species 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- 235000013312 flour Nutrition 0.000 abstract 3
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- IOJNPSPGHUEJAQ-UHFFFAOYSA-N n,n-dimethyl-4-(pyridin-2-yldiazenyl)aniline Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=CC=N1 IOJNPSPGHUEJAQ-UHFFFAOYSA-N 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Abstract
The invention relates to the field of edible fungi culture, and in particular relates to a preservation culture medium for a hypsizygus marmoreus stock culture and a method for preparing the preservation culture medium. Firstly, the preservation culture medium for the hypsizygus marmoreus stock culture is provided, and has a culture medium formula by weight percent as follows: 50-55% of wood flour, 30%-40% of bran, 0.5%-1% of lime powder and 5%-10% of agar. The method for preparing the preservation culture medium is that the wood flour and the bran are mixed according to the proportion to be ground into particles, and then, the lime powder is added to mix uniformly; and the agar is heated to melt and then poured into the mixture of the wood flour, the bran and the lime powder, and water is appropriately added to make the water content of the mixture to be 55-60%. The hypsizygus marmoreus stock culture medium which is prepared by the method is simple and convenient to prepare and low in cost, and has longer preservation time; and moreover, the fungi activity is more strongly kept, the inoculated fungi has quick spawn running, disease resistance and higher heterozygosity resistance, and higher fungi yield of a single bottle is achieved.
Description
Technical field
The present invention relates to field of edible fungus culture, specifically is the female storage medium and preparation method thereof of planting of a kind of Hypsizygus marmoreus.
Background technology
At present, people generally use the PADA substratum when the female kind of preservation Hypsizygus marmoreus, under 4-8 ℃ of temperature, carry out preservation.But its making is loaded down with trivial details, and the shelf time is shorter, is prone to the aging of bacterial classification taken place and degenerate, and causes unpredicted loss to production, is seriously restricting the production of Hypsizygus marmoreus and the benefit of enterprise.
Summary of the invention
The objective of the invention is to provide a kind of simple for production, shelf time long, be fit to Hypsizygus marmoreus and keep the female storage medium and preparation method thereof of planting of active Hypsizygus marmoreus.
At first provide a kind of Hypsizygus marmoreus female storage medium of planting, the weight percent of its culture medium prescription is: wood chip 50-55%, and wheat bran 30%～40%, lime powder 0.5%～1%, agar 5%-10%, and the weight percent sum of each component is 100%.
Contained in the prescription the xylogen, trace element etc. of a large amount of useful Hypsizygus marmoreus growth can satisfy the female basic growth needs of planting of Hypsizygus marmoreus; The original activity that has kept bacterial classification again; It is fast that the test tube of switching is sent out bacterium, and substrate mycelium is flourishing, and the Hypsizygus marmoreus stature of producing is big, weight sufficient, fast growth; Nutritious, the pure white delicacy of the Hypsizygus marmoreus that grows, delicious flavour.
Next provides the female preparation method who plants storage medium of above-mentioned Hypsizygus marmoreus: get wood chip in proportion, wheat bran is ground into particle after mixing, it is even to add lime powder and stirring then; With pouring into after the agar heating and melting in the mixture of wood chip, wheat bran and lime powder, an amount of moisturizing to mixture water cut is 55～60%.
Optimal way is:
Particle size after said wood chip, wheat bran are pulverized is 120 orders; The said adding lime powder and stirring time is 65 minutes.
Behind the medium sterilization after the described moisturizing 2-4 ℃ of preservations; Said culture medium after sterilization pH value is 6.1～6.5.Be kept in the refrigerator in 2-4 ℃ of scopes, can reduce its metabolic speed effectively, the shelf time is longer, has reduced because cellular replication expands numerous spawn degeneration variation that brings etc.
Compared with prior art, the present invention is owing to taked above-mentioned prescription and preservation condition, and the female culture material of planting of the Hypsizygus marmoreus of preparation is simple for production; With low cost, the shelf time is longer; And it is stronger that spawn activity keeps, and the bacterial classification of being transferred sends out that bacterium is fast, disease resistance, anti-polygamy are higher, and single bottle of moushroom yield is higher; Comprehensive benefit is higher, has reached the object of the invention.
Embodiment
Below in conjunction with specific embodiment the present invention is done further explanation.
Embodiment 1
Culture medium prescription is: wood chip 53%, wheat bran 40%, lime powder 0.5%, agar 6.5%.Described per-cent is weight percentage.
Get wood chip in proportion, wheat bran mixes, and pours the particle that is ground in the disintegrating machine about 120 orders into, add lime powder then and put into stirrer and stirred 65 minutes, an amount of moisturizing, to water cut be 55～60%.Sterilization back pH value is 6.2～6.5, is kept at after the inoculation in 3 ℃ of refrigerators.
Embodiment 2:
Culture medium prescription is: wood chip 50%, wheat bran 40%, lime powder 0.5%, agar 9.5%.Described per-cent is weight percentage.
Get wood chip in proportion, wheat bran mixes, and pours the particle that is ground in the disintegrating machine about 120 orders into, add lime powder then and put into stirrer and stirred 65 minutes, an amount of moisturizing, to water cut be 56～58%.Sterilization back pH value is 6.0～6.4, is kept at after the inoculation in 2 ℃ of refrigerators.
Embodiment 3:
Culture medium prescription is: wood chip 55%, wheat bran 34.5%, lime powder 1%, agar 9.5%.Described per-cent is weight percentage.
Get wood chip in proportion, wheat bran mixes, and pours the particle that is ground in the disintegrating machine about 120 orders into, add lime powder then and put into stirrer and stirred 65 minutes, an amount of moisturizing, to water cut be 56～58%.Sterilization back pH value is 6.0～6.4, is kept at after the inoculation in 3 ℃ of refrigerators.
Embodiment 4:
Culture medium prescription is: wood chip 55%, wheat bran 39%, lime powder 1%, agar 5%.Described per-cent is weight percentage.
Get wood chip in proportion, wheat bran mixes, and pours the particle that is ground in the disintegrating machine about 120 orders into, add lime powder then and put into stirrer and stirred 65 minutes, an amount of moisturizing, to water cut be 56～58%.Sterilization back pH value is 6.0～6.4, is kept at after the inoculation in 4 ℃ of refrigerators.
Use embodiment of the invention 1-4 preparation mother culture media and preservation condition can effectively improve shelf time and maintenance spawn activity; As: the culture material that utilizes the present invention to prepare is preserved the Hypsizygus marmoreus bacterial classification; After 150-180 days, expand numerous acquisition and have the original seed that is higher than normal spawn activity.
Claims (4)
1. a Hypsizygus marmoreus mother plants storage medium, it is characterized in that the weight percent of culture medium prescription is: wood chip 50-55%, and wheat bran 30%～40%, lime powder 0.5%～1%, agar 5%-10%, and the weight percent sum of each component is 100%.
2. the female preparation method who plants storage medium of the described Hypsizygus marmoreus of claim 1 is characterized in that, gets wood chip in proportion, wheat bran is ground into particle after mixing, and it is even to add lime powder and stirring then; With pouring into after the agar heating and melting in the mixture of wood chip, wheat bran and lime powder, an amount of moisturizing to mixture water cut is 55～60%.
3. the female preparation method who plants storage medium of Hypsizygus marmoreus according to claim 2 is characterized in that, the particle size after said wood chip, wheat bran are pulverized is 120 orders; The said adding lime powder and stirring time is 65 minutes.
4. according to claim 2 or the female preparation method who plants storage mediums of 3 described Hypsizygus marmoreus, it is characterized in that, behind the medium sterilization after the described moisturizing 2-4 ℃ of preservations; Said culture medium after sterilization pH value is 6.1～6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210353826.7A CN102816012B (en) | 2012-09-21 | 2012-09-21 | Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210353826.7A CN102816012B (en) | 2012-09-21 | 2012-09-21 | Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium |
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| Publication Number | Publication Date |
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| CN102816012A true CN102816012A (en) | 2012-12-12 |
| CN102816012B CN102816012B (en) | 2014-03-26 |
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| CN201210353826.7A Expired - Fee Related CN102816012B (en) | 2012-09-21 | 2012-09-21 | Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255736A (en) * | 2015-11-19 | 2016-01-20 | 天津绿圣蓬源农业科技开发有限公司 | Preservation method of hypsizigus marmoreus strain |
| CN105347855A (en) * | 2015-11-19 | 2016-02-24 | 天津绿圣蓬源农业科技开发有限公司 | Hypsizygus marmoreus strain medium and preparation method thereof |
| CN108315261A (en) * | 2018-04-13 | 2018-07-24 | 四川省农业科学院土壤肥料研究所 | A kind of edible mushroom storage medium and preparation method thereof |
| CN109735454A (en) * | 2019-02-22 | 2019-05-10 | 天津农学院 | A kind of sawdust storage medium and its method for preserving for true pleurotus cornucopiae strain |
| CN112042472A (en) * | 2020-09-28 | 2020-12-08 | 上海市农业科学院 | Culture medium for slowing down aging of hypsizigus marmoreus plate culture |
| CN112493056A (en) * | 2020-11-26 | 2021-03-16 | 河南省蚕业科学研究院 | A composition for preserving Ganoderma leucocontextum strain, its preparation method and inoculation method |
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| JPH06298586A (en) * | 1993-02-22 | 1994-10-25 | Satoru Suga | Production of microbial organic fertilizer |
| CN1754957A (en) * | 2004-09-27 | 2006-04-05 | 广东省微生物研究所 | Method for medium- to long-term preservation of bacteria strain |
| CN101395993A (en) * | 2007-09-29 | 2009-04-01 | 孟桂娟 | Method for planting almond abalone mushroom |
| CN101874453A (en) * | 2010-04-28 | 2010-11-03 | 潘新华 | Culture method of snow white mushroom strains and sporocarps |
| CN102676389A (en) * | 2012-01-04 | 2012-09-19 | 河南科技大学 | Edible fungi strain preservation method and device facilitating taking strains at any time |
-
2012
- 2012-09-21 CN CN201210353826.7A patent/CN102816012B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06298586A (en) * | 1993-02-22 | 1994-10-25 | Satoru Suga | Production of microbial organic fertilizer |
| CN1754957A (en) * | 2004-09-27 | 2006-04-05 | 广东省微生物研究所 | Method for medium- to long-term preservation of bacteria strain |
| CN101395993A (en) * | 2007-09-29 | 2009-04-01 | 孟桂娟 | Method for planting almond abalone mushroom |
| CN101874453A (en) * | 2010-04-28 | 2010-11-03 | 潘新华 | Culture method of snow white mushroom strains and sporocarps |
| CN102676389A (en) * | 2012-01-04 | 2012-09-19 | 河南科技大学 | Edible fungi strain preservation method and device facilitating taking strains at any time |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255736A (en) * | 2015-11-19 | 2016-01-20 | 天津绿圣蓬源农业科技开发有限公司 | Preservation method of hypsizigus marmoreus strain |
| CN105347855A (en) * | 2015-11-19 | 2016-02-24 | 天津绿圣蓬源农业科技开发有限公司 | Hypsizygus marmoreus strain medium and preparation method thereof |
| CN108315261A (en) * | 2018-04-13 | 2018-07-24 | 四川省农业科学院土壤肥料研究所 | A kind of edible mushroom storage medium and preparation method thereof |
| CN109735454A (en) * | 2019-02-22 | 2019-05-10 | 天津农学院 | A kind of sawdust storage medium and its method for preserving for true pleurotus cornucopiae strain |
| CN109735454B (en) * | 2019-02-22 | 2022-03-25 | 天津农学院 | Wood chip preservation medium for hypsizigus marmoreus strains and preservation method thereof |
| CN112042472A (en) * | 2020-09-28 | 2020-12-08 | 上海市农业科学院 | Culture medium for slowing down aging of hypsizigus marmoreus plate culture |
| CN112493056A (en) * | 2020-11-26 | 2021-03-16 | 河南省蚕业科学研究院 | A composition for preserving Ganoderma leucocontextum strain, its preparation method and inoculation method |
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| CN102816012B (en) | 2014-03-26 |
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Address after: Shandong Xinbo 257504 city of Dongying province Kenli County Road No. 88 Applicant after: SHANDONG ZHENGHAN BIOTECHNOLOGY GROUP CO.,LTD. Address before: Shandong Xinbo 257504 city of Dongying province Kenli County Road No. 88 Applicant before: Shandong Zhenghan Biotechnology Group Co.,Ltd. |
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