CN102165945A - Rabbit semen diluent nutrition powder and preparation method thereof - Google Patents
Rabbit semen diluent nutrition powder and preparation method thereof Download PDFInfo
- Publication number
- CN102165945A CN102165945A CN2011100043643A CN201110004364A CN102165945A CN 102165945 A CN102165945 A CN 102165945A CN 2011100043643 A CN2011100043643 A CN 2011100043643A CN 201110004364 A CN201110004364 A CN 201110004364A CN 102165945 A CN102165945 A CN 102165945A
- Authority
- CN
- China
- Prior art keywords
- rabbit
- enrofloxacin
- kanamycin
- glucose
- citric acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses rabbit semen diluent nutrition powder which is prepared from the following components: 2-4 g of tris(hydroxymethyl)aminomethane, 0.5-1.5 g of glucose, 1-3 g of citric acid, 3-8 mg of kanamycin and 3-8 mg of enrofloxacin. The invention also discloses a preparation method of the rabbit semen diluent nutrition powder, which comprises the following steps: (I) weighing raw materials: weighing 2-4 g of tris(hydroxymethyl)aminomethane, 0.5-1.5 g of glucose, 1-3 g of citric acid, 3-8 mg of kanamycin and 3-8 mg of enrofloxacin, and uniformly mixing the raw materials with a vortex mixer to obtain the rabbit semen diluent nutrition powder; and (II) packaging: vacuum-packaging the uniformly mixed rabbit semen diluent powder, and preventing pollution during the entire operation. Through the invention, enough nutrient substances are provided for sperms, the sperm motility maintaining time can be effectively prolonged, and the conception rate of female rabbits is improved.
Description
Technical field
The present invention relates to a kind of rabbit sperm dilution nutrient powder and preparation method thereof.
Background technology
Along with the continuous development of rabbit keeping, select the seminal fluid of good species buck, improve rabbit group's quality and reproduction rate, rabbit development already there is significant meaning.Reaching its maturity of present various domestic animal technology of artificial insemination, level of application are popularized day by day, have more than 20 year though this technology is applied in the meat rabbit production, can't reach widely and use, and one of them important factor is the preservation problem of seminal fluid.Liquid preservation can solve the transportation problem of seminal fluid, extensively provides condition for artificial insemination generally pushes away use, can concentrate and carry out rabbit childbirth and management.
Summary of the invention
The invention provides a kind of rabbit sperm dilution nutrient powder, it not only provides enough nutriments to sperm, carry out the buffering of acid or alkali environment, keep suitable osmotic pressure, suppress the growth of bacterium, and, can effectively prolong the holding time of sperm viability by this rabbit sperm dilution nutrient powder dilution seminal fluid, improve the conception rate of female rabbit, simultaneously easy to use in production application.
The present invention has adopted following technical scheme: a kind of rabbit sperm dilution nutrient powder, it comprises following composition: trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg are formulated.
The content of each composition of the present invention is: trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.
The invention discloses a kind of preparation method of rabbit sperm dilution nutrient powder, it may further comprise the steps:
The first step; Raw material takes by weighing: take by weighing trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg, with above-mentioned raw materials vortex mixer mixing, obtain rabbit sperm dilution nutrient powder; Second step; Packing: the rabbit sperm dilution powder that mixing is good carries out vacuum packaging, prevents to pollute in the whole operation process.
Described first step trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.
The present invention has following beneficial effect: the rabbit sperm dilution nutrient powder of the present invention's preparation, contain trishydroxymethylaminomethane, glucose, citric acid, kanamycin and Enrofloxacin, nutrient powder is diluted with dissolved in distilled water, can carry out liquid state to the rabbit seminal fluid preserves, implement the artificial insemination of rabbit, made time lengthening 84-96 hour that preserves the rabbit seminal fluid, sperm viability is that the effective time-to-live more than 0.4 grade is 48 ± 4,6 hours, the female rabbit conception rate of primiparity was more than 90%, and the female rabbit of multiparity is more than 75%, so not only provide enough nutriments to sperm, carry out the buffering of acid or alkali environment, keep suitable osmotic pressure, suppress the growth of bacterium, and by this rabbit sperm dilution nutrient powder dilution seminal fluid, can effectively prolong the holding time of sperm viability, improve the conception rate of female rabbit, simultaneously easy to use in production application.
Embodiment
The invention discloses a kind of rabbit sperm dilution nutrient powder, it comprises following composition: trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg are formulated.
Further specify this invention below by implementing one.
Embodiment one, the invention provides a kind of rabbit sperm dilution nutrient powder, and it comprises following composition: trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.The present invention makes by the following method: the first step; Raw material takes by weighing: take by weighing trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg, with above-mentioned raw materials vortex mixer mixing, obtain rabbit sperm dilution nutrient powder; Second step; Packing: the rabbit sperm dilution powder that mixing is good carries out vacuum packaging, should make operation room not have dust, ventilation, prevents to pollute rabbit sperm dilution nutrient powder in the whole operation process.
Rabbit sperm dilution powder of the present invention dissolves with the 100ml distilled water, with collection rabbit seminal fluid with 1: 6-10 doubly dilutes (according to seminal fluid density), preserve in 15 ℃ of constant temperature of room temperature, can make the sperm prolonged survival period to 84-96 hour, sperm viability is to be 48 ± 4,6 hours the effective time-to-live more than 0.4 grade.
Rabbit sperm dilution nutrient powder of the present invention is carried out the artificial insemination of Qingdao meat rabbit plant of health larger food Co., Ltd in March, 2010-September, and by 6000 times artificial insemination, the female rabbit conception rate of primiparity is up to 93%, and the female rabbit conception rate of multiparity reaches 78%.
Claims (4)
1. rabbit sperm dilution nutrient powder, it is characterized in that it comprises following composition: trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg are formulated.
2. rabbit sperm dilution nutrient powder according to claim 1 is characterized in that it comprises following composition: trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.
3. the preparation method of a rabbit sperm dilution nutrient powder, it may further comprise the steps:
The first step; Raw material takes by weighing: take by weighing trishydroxymethylaminomethane 2-4g, glucose 0.5-1.5g, citric acid 1-3g, kanamycin 3-8mg and Enrofloxacin 3-8mg, with above-mentioned raw materials vortex mixer mixing, obtain rabbit sperm dilution nutrient powder;
Second step; Packing: the rabbit sperm dilution powder that mixing is good carries out vacuum packaging, prevents to pollute in the whole operation process.
4. the preparation method of rabbit sperm dilution nutrient powder according to claim 1 is characterized in that described first step trishydroxymethylaminomethane 2.8g, glucose 1.2g, citric acid 2.6g, kanamycin 8mg and Enrofloxacin 8mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110004364.3A CN102165945B (en) | 2011-01-11 | 2011-01-11 | Rabbit semen diluent nutrition powder and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110004364.3A CN102165945B (en) | 2011-01-11 | 2011-01-11 | Rabbit semen diluent nutrition powder and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102165945A true CN102165945A (en) | 2011-08-31 |
| CN102165945B CN102165945B (en) | 2014-09-03 |
Family
ID=44487414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110004364.3A Expired - Fee Related CN102165945B (en) | 2011-01-11 | 2011-01-11 | Rabbit semen diluent nutrition powder and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102165945B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112106764A (en) * | 2020-09-25 | 2020-12-22 | 南京农业大学 | Normal-temperature antioxidant rabbit preservation diluent and preparation and use methods thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1927226A (en) * | 2006-09-11 | 2007-03-14 | 华中农业大学 | Cow sex-control sperm freezing preservation seminal fluid dilution |
| CN101611705A (en) * | 2009-07-23 | 2009-12-30 | 于文龙 | A kind of diluting boar semen powder and preparation method thereof |
-
2011
- 2011-01-11 CN CN201110004364.3A patent/CN102165945B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1927226A (en) * | 2006-09-11 | 2007-03-14 | 华中农业大学 | Cow sex-control sperm freezing preservation seminal fluid dilution |
| CN101611705A (en) * | 2009-07-23 | 2009-12-30 | 于文龙 | A kind of diluting boar semen powder and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《动物医学进展》 20020120 茹达干等 "不同稀释液对猪精液常温保存效果的研究" 第77页第3.2节 1-4 第23卷, 第1期 * |
| 茹达干等: ""不同稀释液对猪精液常温保存效果的研究"", 《动物医学进展》, vol. 23, no. 1, 20 January 2002 (2002-01-20) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112106764A (en) * | 2020-09-25 | 2020-12-22 | 南京农业大学 | Normal-temperature antioxidant rabbit preservation diluent and preparation and use methods thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102165945B (en) | 2014-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103493718B (en) | A kind of greening soilless culture substrate and preparation method thereof | |
| CN104671881A (en) | Vegetable soilless culture nutrient solution and manufacturing method thereof | |
| CN101849572B (en) | Method for keeping bamboo shoots fresh | |
| CN101263837A (en) | Composite antistaling agent for citrus shatangju | |
| CN104446918A (en) | Method for manufacturing soilless culture substrate by utilizing apple tree fruit drop | |
| CN102369955A (en) | Preparation for controlling vegetable insect disease | |
| CN102771472B (en) | Freezing liquid for preserving embryo, preparation method and application thereof | |
| CN102424432A (en) | Artificial seawater preparation as well as preparation method and use method thereof | |
| CN102308873A (en) | Orange antistaling agent, its preparation method and application | |
| CN101112147A (en) | Safe hoarding method for Chinese bandoline wood seeds | |
| CN102816012A (en) | Preservation culture medium for hypsizygus marmoreus stock culture and method for preparing preservation culture medium | |
| CN102165945B (en) | Rabbit semen diluent nutrition powder and preparation method thereof | |
| CN102503714B (en) | Special fertilizer for transplanting tobacco | |
| CN111685102A (en) | Sheep sperm solution diluent powder | |
| CN102258007A (en) | Refrigerant and method for storing Pacific codfish sperms | |
| CN108892594A (en) | A kind of plant nutrition liquid enhancing sprigging survival rate | |
| CN104529640A (en) | Leaf vegetable special fertilizer containing amino acid and preparing method thereof | |
| CN104641921A (en) | Japanese bigleaf magnolia leaf cutting propagation method | |
| JP2005261413A (en) | Animal embryo freezing and storing liquid and animal embryo freezing and storing method using the same | |
| CN103103143A (en) | Nitrifying bacteria preservation method | |
| WO2011072201A3 (en) | Silica based plant growth medium | |
| CN105165628A (en) | Laminaria brown seaweed introduction method based on gametophyte cloning line | |
| CN102258006A (en) | Method for storing spermatophore of litopenaeus vannamei at low temperature | |
| CN110583630A (en) | Donkey semen diluent and preparation method thereof | |
| CN102765992B (en) | Fluid fertilizer for seedling culture and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: ZHANG HAIRONG ZHANG HAITAO Effective date: 20140807 Owner name: JIANGSU AGRI-ANIMAL HUSBANDRY VOCATIONAL COLLEGE Free format text: FORMER OWNER: RUIFANG DAN Effective date: 20140807 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20140807 Address after: 225300 Taizhou Fenghuang Road, Jiangsu, No. 8 Applicant after: Jiangsu Agri-Animal Husbandry Vocational College Address before: 225300 Taizhou Fenghuang Road, Jiangsu, No. 8 Applicant before: Dan Ruifang Applicant before: Zhang Hairong Applicant before: Zhang Haitao |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140903 Termination date: 20190111 |