CN101611705A - A kind of diluting boar semen powder and preparation method thereof - Google Patents
A kind of diluting boar semen powder and preparation method thereof Download PDFInfo
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- CN101611705A CN101611705A CN200910012673A CN200910012673A CN101611705A CN 101611705 A CN101611705 A CN 101611705A CN 200910012673 A CN200910012673 A CN 200910012673A CN 200910012673 A CN200910012673 A CN 200910012673A CN 101611705 A CN101611705 A CN 101611705A
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Abstract
The method for making of diluting boar semen powder, get earlier powdered glucose 45～55 grams respectively, Sodium Citrate, usp, Dihydrate Powder 2.5～3.5 grams, disodium salt sodium 0.8～1.2 gram, penicillin 50～800,000 units, dihydrostreptomycin 50～1,000,000 units, get chicken with yolk dry powder 2.5～3 grams again, the above-mentioned powdered glucose that takes out according to quantity, chicken with yolk dry powder, Sodium Citrate, usp, Dihydrate Powder, disodium salt sodium, penicillin and dihydrostreptomycin mix, obtain the diluting boar semen powder, the preparation method of above-mentioned chicken with yolk dry powder is: fresh ovum gallinaceum is boiled, take out chicken with yolk, be dried to the chicken with yolk dry powder of water content 7%～10%.Chicken with yolk dry powder is arranged among the present invention, and its abundant lecithin can ensure the sperm eubolism, after the time of lower limit one fixed length of storage temperature, can play nutrition and the effect of anti-sperm shock.Be not less than 5 days with the pig seminal fluid storage life after the present invention's dilution; The accurate tire rate of using the present invention to dilute the back seminal fluid can reach more than 95%, and a farrowing number can reach 11.6.
Description
Technical field
The present invention relates to a kind of diluting boar semen powder that the diluting boar semen agent is used, particularly a kind of composition of diluting boar semen powder and preparation method of preparing.
Background technology
Boar is in the seminal fluid of once ejaculation, the number of sperm that comprises is far away more than the required quantity of sow fertilization, in the artificial insemination process of pig, available diluting boar semen agent is with many times of diluting boar semens, pig seminal fluid after the dilution has not only increased capacity, also can make the seminal fluid short-term even preserve use for long periods, so that be more sow semen deposition, thereby improve the breeding efficiency of stock boar, improve the reproduction rate of outstanding stock boar greatly.Can use the diluting boar semen liquid of diluting boar semen powder and distilled water preparation during dilution pig seminal fluid.The diluting boar semen powder is a powdery product, nutritious dose, thinner, buffer, Ph conditioning agent and antibiotic in the composition of diluting boar semen liquid, nutritional agents is generally used glucose, fructose, thinner generally with wait the sodium chloride ooze, glucose, really, sucrose and some saline solution, buffer is generally used sodium citrate, sodium hydrogen phosphate, potassium dihydrogen phosphate etc., the Ph conditioning agent is generally used disodium salt sodium, and antibiotic is generally used penicillin, streptomycin, gentamicin, cillimycin etc.Seminal fluid after the dilution need be preserved in temperature is 15-20 ℃ constant temperature refrigerator.Seminal fluid after the dilution is in 15-20 ℃ the constant temperature refrigerator in the preservation process in temperature, and when temperature was low, sperm was subjected to low temperature stimulation easily, even the temperature shock occurs.In the prior art, do not prevent the composition of sperm shock in the diluting boar semen powder, with the pig seminal fluid of the diluting boar semen dilution agent of this diluting boar semen powder preparation in the constant temperature refrigerator in the preservation process, be in time of lower limit one fixed length of storage temperature when temperature after, sperm can produce shock, and sperm produces shock will influence result of use.
Summary of the invention
The objective of the invention is in order to overcome above-mentioned deficiency, propose a kind of pig seminal fluid after can preventing to dilute and produce diluting boar semen powder of sperm shock and preparation method thereof at the lower limit of storage temperature.
The present invention is achieved using the following method.
The diluting boar semen powder is a powdery product, and the composition of diluting boar semen powder and the amount of each composition are:
Powdered glucose: 45～55 grams, chicken with yolk dry powder: 2.5～3 grams, Sodium Citrate, usp, Dihydrate Powder: 2.5～3.5 grams, disodium salt sodium 0.8～1.2 gram, penicillin 50～800,000 units, dihydrostreptomycin 50～1,000,000 units.
The preparation method of diluting boar semen powder has the following steps:
1. get powdered glucose 45～55 grams respectively, Sodium Citrate, usp, Dihydrate Powder 2.5～3.5 grams, disodium salt sodium 0.8～1.2 gram, penicillin 50～800,000 units, dihydrostreptomycin 50～1,000,000 units;
2. preparation chicken with yolk dry powder, the yolk process for preparation of dry powder is: get fresh ovum gallinaceum, fresh ovum gallinaceum is boiled, the fresh chicken with yolk that taking-up is boiled is dried to the chicken with yolk dry powder of water content 7%～10% to chicken with yolk, obtains chicken with yolk dry powder,
3. get chicken with yolk dry powder 2.5～3 grams;
4. above-mentioned powdered glucose, chicken with yolk dry powder, Sodium Citrate, usp, Dihydrate Powder, disodium salt sodium, penicillin and the dihydrostreptomycin that takes out according to quantity mixed, obtain the diluting boar semen powder.
The diluting boar semen powder can adopt and pack storage, and the pig of above-mentioned amount can be packaged into a bag with the sperm dilution powder.After product of the present invention adopts and packs, preserve, under 5～20 ℃ of conditions, can preserve 1 year, under 0～-10 ℃ of condition, can preserve 2 years in shady and cool dry place sealing,
During use,
1. an above-mentioned bag diluting boar semen powder is added in 1000 ml distilled waters, be preheating to 33～35 ℃, shake gently and make it whole dissolvings, become nutrient solution;
2. gather the pig seminal fluid, the pig seminal fluid is placed in the container, nutrient solution is slowly poured in the pig seminal fluid that has just collected along container inner wall, the weight ratio that makes pig seminal fluid and nutrient solution is 1: 2, becomes diluting boar semen liquid for the first time;
3. diluting boar semen liquid left standstill 10 minutes for the first time;
4. add nutrient solution in the first time again in the diluting boar semen liquid, carry out the dilution of required multiple for the second time, allow the ratio of boar semen and nutrient solution be 1: 3～10, become diluting boar semen liquid for the second time;
5. the handle diluting boar semen liquid second time packs by 50～80 milliliters every part, stores under 15～20 ℃ temperature, and every day, taking-up was rocked 1～2 time, can preserve 5 days.
With in the diluting boar semen agent of the present invention preparation nutritious dose: glucose, thinner: glucose solution, buffer: Sodium Citrate, usp, Dihydrate Powder, Ph conditioning agent diamino tetraacethyl sodium and antibiotic penicillin and dihydrostreptomycin, prevent the agent of sperm shock in addition in addition: chicken with yolk dry powder, contain more rich lecithin in the chicken with yolk, the melting point of lecithin is low, easy freezing not at low temperatures, after entering in the sperm body, can ensure normally carrying out of sperm metabolism, after the time of lower limit one fixed length of storage temperature, also can play nutrition well and prevent the effect of sperm shock, thereby safeguard the existence of sperm, make the pig seminal fluid holding time after the dilution long, result of use is good.Be not less than 5 days with the pig seminal fluid storage life after the diluting boar semen powder dilution of the present invention's proposition; The accurate tire rate of using the present invention to dilute the back seminal fluid can reach more than 95%, and a farrowing number can reach 11.6.
Below in conjunction with embodiment, the present invention is further described.
Embodiment
Embodiment 1
The diluting boar semen powder is a powdery product, and the composition of diluting boar semen powder and the amount of each composition are:
Powdered glucose: 50 grams, chicken with yolk dry powder: 3 grams, Sodium Citrate, usp, Dihydrate Powder: 3 grams, disodium salt sodium 1.2 grams, penicillin 800,000 units, dihydrostreptomycin 1,000,000 units.
Embodiment 2
The preparation method of diluting boar semen powder has the following steps:
1. get powdered glucose 50 grams respectively, Sodium Citrate, usp, Dihydrate Powder 3 grams, disodium salt sodium 1.2 grams, penicillin 800,000 units, dihydrostreptomycin 1,000,000 units;
2. preparation chicken with yolk dry powder, the yolk process for preparation of dry powder is: get fresh ovum gallinaceum, fresh ovum gallinaceum is boiled, the fresh chicken with yolk that taking-up is boiled is dried to the chicken with yolk dry powder of water content 7%～10% to chicken with yolk, obtains chicken with yolk dry powder,
3. get chicken with yolk dry powder 3 grams;
4. above-mentioned powdered glucose, chicken with yolk dry powder, Sodium Citrate, usp, Dihydrate Powder, disodium salt sodium, penicillin and the dihydrostreptomycin that takes out according to quantity mixed, obtain the diluting boar semen powder.
Claims (4)
1. diluting boar semen powder, it is powdery product, powdered glucose, Sodium Citrate, usp, Dihydrate Powder, disodium salt sodium, penicillin and dihydrostreptomycin are arranged in the composition of diluting boar semen powder, it is characterized in that: also have chicken with yolk dry powder in the composition of diluting boar semen powder, the amount of each composition is in the diluting boar semen powder: powdered glucose: 45～55 grams, chicken with yolk dry powder: 2.5～3 grams, Sodium Citrate, usp, Dihydrate Powder: 2.5～3.5 grams, disodium salt sodium 0.8～1.2 gram, penicillin 50～800,000 units, dihydrostreptomycin 50～1,000,000 units.
2. by the described diluting boar semen powder of claim 1, it is characterized in that: the amount of each composition is in the described diluting boar semen powder: powdered glucose: 50 grams, chicken with yolk dry powder: 3 grams, Sodium Citrate, usp, Dihydrate Powder: 3 grams, disodium salt sodium 1.2 grams, penicillin 800,000 units, dihydrostreptomycin 1,000,000 units.
3. preparation method by the described diluting boar semen powder of claim 1 has the following steps:
Get earlier powdered glucose 45～55 grams respectively, Sodium Citrate, usp, Dihydrate Powder 2.5～3.5 grams, disodium salt sodium 0.8～1.2 gram, penicillin 50～800,000 units, dihydrostreptomycin 50～1,000,000 units, it is characterized in that: get chicken with yolk dry powder 2.5～3 grams again, the above-mentioned powdered glucose that takes out according to quantity, chicken with yolk dry powder, Sodium Citrate, usp, Dihydrate Powder, disodium salt sodium, penicillin and dihydrostreptomycin mix, obtain the diluting boar semen powder, the preparation method of above-mentioned chicken with yolk dry powder is: get fresh ovum gallinaceum, fresh ovum gallinaceum is boiled, take out the fresh chicken with yolk of boiling, chicken with yolk is dried to the chicken with yolk dry powder of water content 7%～10%, obtains chicken with yolk dry powder.
4. press the preparation method of the described diluting boar semen powder of claim 3, it is characterized in that: the amount of described powdered glucose, chicken with yolk dry powder, Sodium Citrate, usp, Dihydrate Powder, disodium salt sodium, penicillin and dihydrostreptomycin is respectively: powdered glucose: 50 grams, chicken with yolk dry powder: 3 grams, Sodium Citrate, usp, Dihydrate Powder: 3 grams, disodium salt sodium 1.2 grams, penicillin 800,000 units, dihydrostreptomycin 1,000,000 units.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856016A (en) * | 2010-06-19 | 2010-10-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN102165945A (en) * | 2011-01-11 | 2011-08-31 | 淡瑞芳 | Rabbit semen diluent nutrition powder and preparation method thereof |
| CN102726371A (en) * | 2012-07-11 | 2012-10-17 | 于文龙 | Long-acting boar semen diluting powder and preparation method thereof |
| CN105794770A (en) * | 2016-04-22 | 2016-07-27 | 李志新 | Special diluent for boar semen |
| CN108077244A (en) * | 2018-01-31 | 2018-05-29 | 于文龙 | A kind of long-acting boar semen dilution powder |
| CN109221085A (en) * | 2018-09-25 | 2019-01-18 | 上海祥欣畜禽有限公司 | The fresh essence of pig saves dilution powder |
| CN110447635A (en) * | 2019-07-17 | 2019-11-15 | 西北农林科技大学 | Polysaccharide material is used to prepare the application and preparation method of goat sperm freezen protective dilution |
-
2009
- 2009-07-23 CN CN200910012673A patent/CN101611705A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856016A (en) * | 2010-06-19 | 2010-10-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN101856016B (en) * | 2010-06-19 | 2013-03-13 | 张尤嘉 | Diluent for preserving porcine semen at normal temperature |
| CN102165945A (en) * | 2011-01-11 | 2011-08-31 | 淡瑞芳 | Rabbit semen diluent nutrition powder and preparation method thereof |
| CN102165945B (en) * | 2011-01-11 | 2014-09-03 | 江苏农牧科技职业学院 | Rabbit semen diluent nutrition powder and preparation method thereof |
| CN102726371A (en) * | 2012-07-11 | 2012-10-17 | 于文龙 | Long-acting boar semen diluting powder and preparation method thereof |
| CN105794770A (en) * | 2016-04-22 | 2016-07-27 | 李志新 | Special diluent for boar semen |
| CN108077244A (en) * | 2018-01-31 | 2018-05-29 | 于文龙 | A kind of long-acting boar semen dilution powder |
| CN109221085A (en) * | 2018-09-25 | 2019-01-18 | 上海祥欣畜禽有限公司 | The fresh essence of pig saves dilution powder |
| CN110447635A (en) * | 2019-07-17 | 2019-11-15 | 西北农林科技大学 | Polysaccharide material is used to prepare the application and preparation method of goat sperm freezen protective dilution |
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Open date: 20091230 |