CN103392692A - Cryopreservation solution for goat semen - Google Patents
Cryopreservation solution for goat semen Download PDFInfo
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- CN103392692A CN103392692A CN201310354478XA CN201310354478A CN103392692A CN 103392692 A CN103392692 A CN 103392692A CN 201310354478X A CN201310354478X A CN 201310354478XA CN 201310354478 A CN201310354478 A CN 201310354478A CN 103392692 A CN103392692 A CN 103392692A
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Abstract
The invention relates to a cryopreservation solution for goat semen, belonging to the field of sperm preservation. The cryopreservation solution comprises 75ml of diluent, 20ml of yolk liquid, 5ml of glycol and 10ml of antibiotics. The cryopreservation solution has the effects that the sperm motility rate and acrosome integrity rate of semen after cryopreservation are increased, meanwhile, the oestrus conception rate of goats during artificial insemination is increased, and the reproductive performance and economic benefit of the goats are improved remarkably.
Description
Technical field
The present invention relates to a kind of seminal fluid and preserve liquid, and be specifically related to the freezing preservation liquid of a kind of goat sperm.
Background technology
Semen cryopreservation is the key technique of goat technology of artificial insemination.It has solved the problem of seminal fluid long preservation, makes seminal fluid not be subjected to the restriction of time, region and breeding stock life, brings into play greatly and improve the availability of good species ram, accelerates incubation and the improvement paces of kind.Simultaneously, the good species ram is carried out to progeny testing in short-term, keeps and recover the outstanding selected characteristic of a certain kind or individual ram, and carrying out blood lineage's renewal, to introduce a fine variety, lower the aspects such as production cost significant.The principle of freezing preservation is to preserve sperm under condition of ultralow temperature, and the metabolism of sperm and energy are stopped substantially, therefore can long preservation.The freezing Techniques of preserving of goat sperm, just have the experimental study of many countries abroad, and succeed since the sixties in 20th century.China carried out the goat sperm refrigeration test and succeeded the seventies, promoted the development that China goat is frozen smart technology, and the test of some areas shows, its non-return rate can reach 60%.Along with the extensive use of technology of artificial insemination, 20th century livestock semen Techniques of preserving produced thereupon.Due to the restriction that is subjected to the factors such as time, region and age, and introduce the live expense of breeding stock and some potential safety problems, therefore, freeze Semen freezing technique and become the important means that changes this situation.
Summary of the invention
The technical problem that the present invention mainly solves is to provide the freezing preservation liquid of a kind of goat sperm, can improve the effect of sperm motility rate after semen cryopreservation, acrosomal integrity, improve simultaneously the test-tube non-return rate of goat, made the reproductive performance of goat and the effect that economic benefit is significantly increased.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is the freezing preservation liquid of a kind of goat sperm, and it comprises dilution 75ml, yolk liquid 20ml, ethylene glycol 5ml, antibiotic 10ml.
The every 100ml of described dilution comprises 3g glucose, 0.02g Tris, 0.3g EDTA, 3.7g sodium citrate, 0.1g bovine serum albumin(BSA), 0.06g VE.
Described antibiotic is penicillin and streptomycin, and its content is respectively 50000IU/10ml.
In the additive that uses in the present invention:
Vitamin: 1976, the vigor that vitamin E can improve the sperm that thaws was added in the discoveries such as Vamawskij AN in the sheep semen diluent.After this people find by more test, in seminal fluid, add the physicochemical property that vitamin substances can also improve the sperm external environment, the fertility of sperm while improving artificial insemination, wherein cobalamin vitamin E and ascorbic research and utilize more.
Vitamin E has strong reducing property, participates in the redox reaction in body.Research is found, in sperm freezing dilution liquid, adds the vigor that vitamin E can significantly improve the sperm that thaws.
Buffer substance: the freeze-extender of seminal fluid should have certain buffer capacity, and reason is that sperm produces in metabolic process some materials can make the pH value of dilution depart from, and sperm is had a negative impact.We adopt sodium citrate to keep the suitable pH value of seminal fluid, and the suitableeest pH value of dilution is 7.0～7.5.
The osmotic pressure of sperm freezing dilution liquid is also the key factor that affects thaw sperm motility rate and fertilization rate.Sperm is pressed with certain tolerance range to infiltration.In 50%～150% scope of normal tension, sperm is subjected to impact that height oozes not as hypotonic so large, and the osmotic pressure of sperm freezing dilution liquid is generally etc. and oozes or moderate hypertonic solution.
Antibiotic: often add different types of antibiotic in the domestic animal semen diluent, mainly because can be polluted by various bacteria unavoidably in the semen collection process, although some bacterium has no adverse effects to seminal fluid, but most bacteriums, especially Escherichia coli preserve impact greatly to seminal fluid, and sperm viability, acrosomal integrity, pH value are significantly descended.We adopt mycillin to prevent that the germ contamination in freezing liquid from having obtained good effect.
The invention has the beneficial effects as follows: this formula has the effect that improves sperm motility rate after semen cryopreservation, acrosomal integrity, has improved simultaneously the test-tube non-return rate of goat, makes the reproductive performance of goat and the effect that economic benefit is significantly increased.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
The embodiment of the present invention comprises:
The freezing preservation liquid of a kind of goat sperm, it comprises dilution 75ml, yolk liquid 20ml, ethylene glycol 5ml, antibiotic 10ml.The every 100ml of described dilution comprises 3g glucose, 0.02g Tris, 0.3g EDTA, 3.7g sodium citrate, 0.1g bovine serum albumin(BSA), 0.06g VE.Described antibiotic is penicillin and streptomycin, and its content is respectively 50000IU/10ml.
Select wean Xu Huai he-goat as subjects, refer to following table:
Experimental group and control group are in freezing front sperm motility rate difference not significantly (p<0.5), and after thawing, on sperm motility rate, acrosomal integrity, three measurement indexs of non-return rate, experimental group is all higher than control group significant difference (p>0.5).
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in scope of patent protection of the present invention.
Claims (3)
1. the freezing preservation liquid of goat sperm, is characterized in that, it comprises dilution 75ml, yolk liquid 20ml, ethylene glycol 5ml, antibiotic 10ml.
2. the freezing preservation liquid of goat sperm according to claim 1, is characterized in that, the every 100ml of described dilution comprises 3g glucose, 0.02g Tris, 0.3g EDTA, 3.7g sodium citrate, 0.1g bovine serum albumin(BSA), 0.06g VE.
3. the freezing preservation liquid of goat sperm according to claim 1 and 2, is characterized in that, described antibiotic is penicillin and streptomycin, and its content is respectively 50000IU/10ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310354478XA CN103392692A (en) | 2013-08-14 | 2013-08-14 | Cryopreservation solution for goat semen |
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| CN201310354478XA CN103392692A (en) | 2013-08-14 | 2013-08-14 | Cryopreservation solution for goat semen |
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| CN103392692A true CN103392692A (en) | 2013-11-20 |
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| CN201310354478XA Pending CN103392692A (en) | 2013-08-14 | 2013-08-14 | Cryopreservation solution for goat semen |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068013A (en) * | 2014-07-17 | 2014-10-01 | 赤峰市农牧科学研究院 | Sheep semen dilution preserving fluid and preparation method thereof |
| CN106922650A (en) * | 2017-03-17 | 2017-07-07 | 内蒙古自治区农牧业科学院 | A kind of down producing goat semen dilution formula of liquid |
| CN109221091A (en) * | 2018-11-01 | 2019-01-18 | 洛宁农本畜牧科技开发有限公司 | Fresh smart low temperature dilution of a kind of animal and the preparation method and application thereof |
| CN110463687A (en) * | 2019-07-10 | 2019-11-19 | 安徽农业大学 | A kind of the preservation liquid and store method of sheep sperm |
| CN110604129A (en) * | 2019-10-28 | 2019-12-24 | 浙江大学 | Hu sheep semen cryopreservation liquid and application thereof |
| CN110881461A (en) * | 2019-12-12 | 2020-03-17 | 中南百草原集团有限公司 | Sheep sperm liquid transportation method |
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| CN102960336A (en) * | 2012-11-09 | 2013-03-13 | 镇江万山红遍农业园 | Frozen sperm diluent of Boer crossbred goats |
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| EP1627565A1 (en) * | 2004-08-10 | 2006-02-22 | Consejo Superior de Investigaciones Cientificas (CSIC) | Use of flavanol derivatives for the cryopreservation of living cells |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068013A (en) * | 2014-07-17 | 2014-10-01 | 赤峰市农牧科学研究院 | Sheep semen dilution preserving fluid and preparation method thereof |
| CN104068013B (en) * | 2014-07-17 | 2016-04-20 | 赤峰市农牧科学研究院 | A kind of sheep sperm dilution conserving liquid and preparation method thereof |
| CN106922650A (en) * | 2017-03-17 | 2017-07-07 | 内蒙古自治区农牧业科学院 | A kind of down producing goat semen dilution formula of liquid |
| CN109221091A (en) * | 2018-11-01 | 2019-01-18 | 洛宁农本畜牧科技开发有限公司 | Fresh smart low temperature dilution of a kind of animal and the preparation method and application thereof |
| CN110463687A (en) * | 2019-07-10 | 2019-11-19 | 安徽农业大学 | A kind of the preservation liquid and store method of sheep sperm |
| CN110463687B (en) * | 2019-07-10 | 2021-07-27 | 安徽农业大学 | Preservation solution and preservation method for sheep semen solution |
| CN110604129A (en) * | 2019-10-28 | 2019-12-24 | 浙江大学 | Hu sheep semen cryopreservation liquid and application thereof |
| CN110881461A (en) * | 2019-12-12 | 2020-03-17 | 中南百草原集团有限公司 | Sheep sperm liquid transportation method |
| CN110881461B (en) * | 2019-12-12 | 2021-10-08 | 中南百草原集团有限公司 | Sheep sperm liquid transportation method |
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Application publication date: 20131120 |