CN105794770A - Special diluent for boar semen - Google Patents

Special diluent for boar semen Download PDF

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Publication number
CN105794770A
CN105794770A CN201610254564.7A CN201610254564A CN105794770A CN 105794770 A CN105794770 A CN 105794770A CN 201610254564 A CN201610254564 A CN 201610254564A CN 105794770 A CN105794770 A CN 105794770A
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CN
China
Prior art keywords
grams
gram
solvent
diluent
trehalose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610254564.7A
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Chinese (zh)
Inventor
李志新
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Individual
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Individual
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Priority to CN201610254564.7A priority Critical patent/CN105794770A/en
Publication of CN105794770A publication Critical patent/CN105794770A/en
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physiology (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a special diluent for boar semen. Sterilized distilled water is used as a solvent; the diluent of every 1,000ml is prepared from the following components: 4 to 6 grams of glycerinum, 1 to 2 grams of soybean lecithin, 10 to 15 grams of trehalose, 2.5 to 3.5 grams of vitamin E, 5 to 10 grams of yolk, 3 to 5 grams of sodium citrate, 600,000 to 800,000 IUs (international units) of penicillin and the balance a solvent; a PH (Potential of Hydrogen) value is regulated to be 6.6 to 7.0. The cryopreservation is carried out on the boar semen by utilizing the diluent provided by the invention, and the viability of sperms after unfreezing is up to above 75 percent.

Description

A kind of special diluent of pig semen
Technical field
The present invention relates to biological technical field, be specifically related to a kind of special diluent of pig semen.
Background technology
Livestock semen Techniques of preserving is that twentieth century is extensively applied along with technology of artificial insemination and given birth to Producing, principle is to reach to extend sperm at body by reduction or Inhibit sperm metabolism degree The purpose in outer life-span.To this end, seminal fluid is placed on low temperature state (non-icing) or cold by researcher test Freeze and preserve under (icing) state.But in temperature changing process, the seminal fluid protective capability to sperm It is limited.Will be with the use of special solution first by essence to this end, carry out semen cryopreservation Liquid is diluted.The basic demand of diluent is included pH value, resiliency, osmotic pressure and low Temperature protective value.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of special diluent of pig semen.
Solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of special diluent of pig semen, with sterile purified water as solvent, every 1000ml dilutes Liquid consists of the following composition: glycerol 4-6 gram, soybean lecithin 1-2 gram, trehalose 10-15 Gram, vitamin E2 .5-3.5 gram, 5-10 gram of yolk, sodium citrate 3-5 gram, penicillin 60-80 ten thousand IU, remaining be solvent, regulation pH value is 6.6-7.0.
Preferably, a kind of special diluent of pig semen, with sterile purified water as solvent, every 1000ml Diluent consists of the following composition: glycerol 5 grams, soybean lecithin 1.5 grams, trehalose 12.5 Gram, vitamin E 3 grams, 7.5 grams of yolk, sodium citrate 4 grams, penicillin 700,000 IU, Remaining is solvent, and regulation pH value is 6.8.
The invention have the advantage that
1, glycerol can penetrate into intracellular, concentrates or combines intracellular moisture, and glycerol also has dilute Release effect, the concentration of salt in solution and the osmotic pressure of freezing liquid can be reduced；Soybean lecithin and ovum Huang maintains facilitation to normal acrosome；Trehalose and vitamin E can improve motile sperm Percentage rate and the ratio of normal acrosome；Sodium citrate is in order to keep the suitable pH value of seminal fluid； Multiplying of the antibiotic substance suppression bacterial micro-organisms such as penicillin.
2, the diluent utilizing the present invention carries out freezen protective to pig semen, and after defrosting, sperm is lived Power reaches more than 75%.
Detailed description of the invention
Below embodiments of the invention are described in detail, so that advantages and features of the invention Can be easier to be readily appreciated by one skilled in the art, thus protection scope of the present invention is made more Explicit defines.
Embodiment 1
A kind of special diluent of pig semen, with sterile purified water as solvent, every 1000ml dilutes Liquid consists of the following composition: glycerol 4 grams, soybean lecithin 1 gram, trehalose 10 grams, dimension Raw element E2.5 gram, 5 grams of yolk, sodium citrate 3 grams, penicillin 600,000 IU, remaining be Solvent, regulation pH value is 6.6.
Embodiment 2
A kind of special diluent of pig semen, with sterile purified water as solvent, every 1000ml dilutes Liquid consists of the following composition: glycerol 5 grams, soybean lecithin 1.5 grams, trehalose 12.5 grams, Vitamin E 3 grams, 7.5 grams of yolk, sodium citrate 4 grams, penicillin 700,000 IU, remaining For solvent, regulation pH value is 6.8.
Embodiment 3
A kind of special diluent of pig semen, with sterile purified water as solvent, every 1000ml dilutes Liquid consists of the following composition: glycerol 6 grams, soybean lecithin 2 grams, trehalose 15 grams, dimension Raw element E3.5 gram, 10 grams of yolk, sodium citrate 5 grams, penicillin 800,000 IU, remaining be Solvent, regulation pH value is 7.0.

Claims (2)

1. the special diluent of pig semen, it is characterised in that with sterile purified water as solvent, Every 1000ml diluent consists of the following composition: glycerol 4-6 gram, soybean lecithin 1-2 gram, Trehalose 10-15 gram, vitamin E2 .5-3.5 gram, 5-10 gram of yolk, sodium citrate 3-5 Gram, penicillin 60-80 ten thousand IU, remaining be solvent, regulation pH value is 6.6-7.0.
The special diluent of pig semen the most according to claim 1, it is characterised in that to go out Bacterium distilled water is solvent, and every 1000ml diluent consists of the following composition: glycerol 5 grams, big 1.5 grams of bean lecithin, trehalose 12.5 grams, vitamin E 3 grams, 7.5 grams of yolk, Fructus Citri Limoniae Acid 4 grams of sodium, penicillin 700,000 IU, remaining be solvent, regulate pH value be 6.8.
CN201610254564.7A 2016-04-22 2016-04-22 Special diluent for boar semen Pending CN105794770A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610254564.7A CN105794770A (en) 2016-04-22 2016-04-22 Special diluent for boar semen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610254564.7A CN105794770A (en) 2016-04-22 2016-04-22 Special diluent for boar semen

Publications (1)

Publication Number Publication Date
CN105794770A true CN105794770A (en) 2016-07-27

Family

ID=56457477

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610254564.7A Pending CN105794770A (en) 2016-04-22 2016-04-22 Special diluent for boar semen

Country Status (1)

Country Link
CN (1) CN105794770A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107094755A (en) * 2017-06-23 2017-08-29 四川农业大学 It is a kind of to improve freeze proof diluent of nutrition that sperm in vitro is preserved and preparation method thereof
CN109221085A (en) * 2018-09-25 2019-01-18 上海祥欣畜禽有限公司 The fresh essence of pig saves dilution powder

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101310729A (en) * 2007-05-23 2008-11-26 辽宁省辽宁绒山羊育种中心 Animal origin free flocks and herds frozen semen diluent and production method of flocks and herds frozen semen
CN101392236A (en) * 2008-11-06 2009-03-25 上海交通大学 Antifreezer for diluting boar semen
CN101595866A (en) * 2008-06-06 2009-12-09 上海市农业科学院 One boar semen and pig seminal fluid processing method
CN101611705A (en) * 2009-07-23 2009-12-30 于文龙 A kind of diluting boar semen powder and preparation method thereof
CN101796945A (en) * 2010-04-12 2010-08-11 西北农林科技大学 Antifreeze used for freezing and preserving boar semens as well as preparation and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101310729A (en) * 2007-05-23 2008-11-26 辽宁省辽宁绒山羊育种中心 Animal origin free flocks and herds frozen semen diluent and production method of flocks and herds frozen semen
CN101595866A (en) * 2008-06-06 2009-12-09 上海市农业科学院 One boar semen and pig seminal fluid processing method
CN101392236A (en) * 2008-11-06 2009-03-25 上海交通大学 Antifreezer for diluting boar semen
CN101611705A (en) * 2009-07-23 2009-12-30 于文龙 A kind of diluting boar semen powder and preparation method thereof
CN101796945A (en) * 2010-04-12 2010-08-11 西北农林科技大学 Antifreeze used for freezing and preserving boar semens as well as preparation and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
广西壮族自治区畜牧研究所: "猪冷冻精液的研制和使用", 《农业科技通讯》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107094755A (en) * 2017-06-23 2017-08-29 四川农业大学 It is a kind of to improve freeze proof diluent of nutrition that sperm in vitro is preserved and preparation method thereof
CN109221085A (en) * 2018-09-25 2019-01-18 上海祥欣畜禽有限公司 The fresh essence of pig saves dilution powder

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Application publication date: 20160727

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