CN109221085A - The fresh essence of pig saves dilution powder - Google Patents
The fresh essence of pig saves dilution powder Download PDFInfo
- Publication number
- CN109221085A CN109221085A CN201811113135.3A CN201811113135A CN109221085A CN 109221085 A CN109221085 A CN 109221085A CN 201811113135 A CN201811113135 A CN 201811113135A CN 109221085 A CN109221085 A CN 109221085A
- Authority
- CN
- China
- Prior art keywords
- pig
- agent
- dilution
- saves
- dilution powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/0231—Chemically defined matrices, e.g. alginate gels, for immobilising, holding or storing cells, tissue or organs for preservation purposes; Chemically altering or fixing cells, tissue or organs, e.g. by cross-linking, for preservation purposes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physiology (AREA)
- Biophysics (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides the fresh essence of a boar and saves dilution powder; it includes energy matter nutritional agents, osmotic pressure regulator, pH adjusting agent and weak acid salt buffer agent that the fresh essence of pig, which saves dilution powder; it wherein further include cryoprotective agent, the cryoprotective agent is trehalose and soybean lecithin.The present invention in dilution powder by adding the sperms cryoprotective agent such as trehalose and soybean lecithin; sperm is protected not suffer a shock under low temperature strike impaired; so that the fresh essence after dilution can be protected in 4~14 DEG C of low temperature regional environment; to solve the preservation transportation problem of pig semen under low temperature season or low temperature environment, so that Semen routine temperature region widens into 4~20 DEG C.
Description
Technical field
The invention belongs to herding breeding fields, are related to the fresh essence of a boar and save dilution powder.
Background technique
Continuous development and boar union breeding requirements of one's work with China's pig breeding industry, swine artificial insemination it is rapid general
And while the swine artificial insemination stations of different scales have become the important component of China's pig industry.Pig semen is after extraction
Needing to be added dilution can save for a long time, and be used for more insemination of sows.Artificial insemination station is in order to sufficiently benefit
With outstanding herd boar, its genetic superiority is played, reaches resource-sharing truly, first has to ensure the sperm matter produced
Amount and preservation, transport effect.Holding time, preservation effect after semen dilution and transport are the key that artificial insemination application success or failure
Therefore factor develops that the holding time is long, preservation effect is good, the advantageous commercialization of cost semen diluent, to people
There is important value and significance for work fertilization technique.
The fresh smart dilution of general pig is made of dilution powder dilutes in pure water, dilution powder by osmotic pressure regulator,
The ingredients such as pH value (pH value) regulator, buffer and nutritional agents are constituted.In the market in the fresh smart dilution powder of common pig, such as in
State patent application CN201510756292.6 generally does osmotic pressure regulator using sodium chloride or potassium chloride, with ethylenediamine tetrem
The chelating agents such as sour sodium (EDTA) do pH adjusting agent, do buffer with salts of weak acid such as sodium citrates, with energy matters such as glucose
Nutritional agents is done, to prevent bacterium infection, the antibiotic such as gentamicin or penicillin and streptomycin can also be added.
But at present using the dilution powder of such formula, due to not adding cryoprotective agent, guarantor of the dilution to pig semen
Depositing temperature region is that cannot save under 15 DEG C or less of environment, Conservation environment temperature region is relatively narrow between 15~20 DEG C.
When needing lower than saving in 15 DEG C of environment below or when transport, sperm after cold strike by can draw at low temperature
Cold shock is played, causes perforatorium, plasma membrane etc. impaired, loses fertilizing ability or death, to make the pig semen insemination after dilution
Ability decline or failure.
Summary of the invention
It is relatively narrow in order to solve the Semen routine temperature range after diluting in existing dilution powder technology, especially cannot 4~
The problem of 14 DEG C of section cryo-conservations, the present invention propose a kind of pig semen that can be applied in 4~20 DEG C of wide temperature regions
Save dilution powder.
The object of the present invention is to provide the fresh essences of a boar to save dilution powder, and it includes energy object that the fresh essence of pig, which saves dilution powder,
Matter nutritional agents, osmotic pressure regulator, pH adjusting agent and weak acid salt buffer agent, which is characterized in that it further include cryoprotective agent, institute
Stating cryoprotective agent is trehalose and soybean lecithin.
Further, the fresh essence of a boar provided by the invention saves dilution powder, further includes antibiotic.
Wherein, the energy matter nutritional agents is powdered glucose；The osmotic pressure regulator is NaCl and/or KCl；
The pH adjusting agent is sodium ethylene diamine tetracetate chelating agent；The weak acid salt buffer agent is sodium citrate.
Again specifically, it is saved in dilution powder in the fresh essence of pig of the invention, to be diluted to required for 1000 milliliters of dilutions
Pig it is fresh essence save dilution powder meter, the energy matter nutritional agents be 20.0~35.0g, the pH adjusting agent be 1.0~
3.0g, the osmotic pressure regulator are 0.2~1.0g, and the weak acid salt buffer agent is 5.0~10.0g, the seaweed
Sugar is 1.0~18.0g, and the soybean lecithin is 1.0~18.0g.
Further, the fresh essence of pig of the invention saves dilution powder, further includes antibiotic, and the antibiotic is penicillin 1.0
~2.0g, 1.0~2.0g of streptomysin.
The fresh essence of pig of the invention saves dilution powder, can prepare as follows:
(1) it saves based on dilution powder by being diluted to the fresh essence of pig required for 1000 milliliters of dilutions, in low temperature, drying, dustless
20.0~35.0g of powdered glucose, disodium salt sodium (EDTA) 1.0~3.0g, potassium chloride or chlorination are weighed under environment respectively
0.2~1.0g of sodium, 5.0~10.0g of sodium citrate, 1.0~2.0g of penicillin, 1.0~2.0g of streptomysin, trehalose 1.0~
18.0g, 1.0~18g of soybean lecithin；
(2) the above ingredient is stirred evenly in low temperature, dry environment, using automatic packer sealed package, is stored in 2
In~20 DEG C of environment.
The fresh essence of pig of the invention saves dilution powder, low by improving resisting for pig semen in terms of the preservation applied to pig semen
Temperature saves transport capacity, so that can do wide temperature region after diluting boar semen between 4~20 DEG C saves transport, to improve pig essence
The survival rate that son saves under prolonged temperature environment can use by the following method:
With pure water, 20~35 DEG C at a temperature of according to above-mentioned parts by weight dissolved dilution powder at 1000mL, then 20
Dilution is obtained after balancing 1 hour in~35 DEG C of water bath with thermostatic control.When dilution pig is fresh smart, the temperature difference control of pig semen and dilution
For system within 2 DEG C, dilution ratio is volume ratio 1:1~1:9.Sperm after dilution is protected from light standing two in 17~25 DEG C of environment
After as a child, be put into 4~20 DEG C of refrigerator and save backup, can be used for example 4~8 DEG C home-use cold storage refrigerator or 17 DEG C
Constant temperature Semen routine special refrigerator saves, and every 12 hours, it is primary to stir sperm during preservation, prevents Sperm pellets.
Advantages of the present invention is as follows:
It is compared with other dilution powders currently popular, the present invention in dilution powder by adding trehalose and soybean lecithin
Equal sperms cryoprotective agent can protect sperm and not suffer a shock under low temperature strike impaired, Boar spermatozoa is enabled to have low-temperature resistance
Power；So that the fresh essence after dilution can be protected in 4~14 DEG C of low temperature regional environment, it is not in that cold shock is suffered a loss
It is living or dead, to solve the preservation transportation problem of pig semen under low temperature season or low temperature environment, so that Semen routine temperature
Region widens into 4~20 DEG C.Moreover, being saved after adding trehalose and soybean lecithin simultaneously in 4~20 DEG C of wide temperature regions
When, vigor and acrosomal integrity to sperm have coordinating protection effect.
Specific embodiment
Below by embodiment, the present invention is further illustrated, but the present embodiment can not limit the present invention.Implement
Raw material used in example is commercially available.
Examples 1 to 3 and comparative example 1~2
The fresh essence of pig saves dilution powder, is powdery product, respectively according to listed in table 1 under low temperature, drying, dustfree environment
Weight weigh, then stirred evenly in low temperature, dry environment and be packaged into 1 packet, be stored in 4 DEG C, 8 DEG C, 10 DEG C, 17 DEG C respectively
With it is stand-by in 20 DEG C of refrigerator.
The formula of table 1, Examples 1 to 3 and comparative example 1~2
Effect experimental examples
It is not added with for five kinds of formulas in comparative example 1~3 and comparative example 1~2 with popular other on Vehicles Collected from Market
The wide temperature region preservation effect of sperm cryoprotective agent boar semen dilution powder is compared provided with control group test, is compareed
Group is higher BTS boar semen dilution powder (the glucose 37g, second diammonium tetrem for being not added with cryoprotective agent of occupation rate in the market
Acid disodium 0.4g, potassium chloride 0.3g, sodium citrate 6.0g, sodium bicarbonate 1.3g.).
Dilution powder in 1 packet Examples 1 to 3 and comparative example 1~2 is used into pure water respectively, in 20~35 DEG C of temperature
Lower dissolved dilution powder, then 1 hour of balance obtains 1 liter of dilution in 20~35 DEG C of water bath with thermostatic control.When dilution pig is fresh smart,
The temperature difference of sperm and dilution controls within 2 DEG C, and dilution ratio volume ratio is 1:5.Sperm after dilution is in 17 DEG C of environment
It after being protected from light standing two as a child, is put into cold storage refrigerator and is saved under 4~9 DEG C and 9~14 DEG C of two temperature regions, save
It is primary that period every 12 hours stir sperm, prevents Sperm pellets.Experimental observation index is sperm motility, acrosomal integnity；Examination
Testing and saving number of days is 7 days；Testing inspection frequency is primary every detection in 2 days during saving.
Vigor testing methods are microscopic visual measurement point system at 37 DEG C in test；Acrosomal integrity detection method uses Jim
Sa decoration method microscopic visual measurement counts.
Test result is as follows:
The vigor comparison that table 2, different dilution powder dilution sperm are saved in 4 to 9 DEG C of temperature regions
(same column data in table, shoulder marking-up parent phase is not significant (P > 0.05) with difference is represented, and it is poor that the female difference of shoulder marking-up represents
Different significant (P < 0.05).)
The acrosomal integrity comparison that table 3, different dilution powder dilution sperm are saved in 4 to 9 DEG C of temperature regions
(same column data in table, shoulder marking-up parent phase is not significant (P > 0.05) with difference is represented, and it is poor that the female difference of shoulder marking-up represents
Different significant (P < 0.05).)
The vigor comparison that table 4, different dilution powder dilution sperm are saved in 9 to 14 DEG C of temperature regions
(same column data in table, shoulder marking-up parent phase is not significant (P > 0.05) with difference is represented, and it is poor that the female difference of shoulder marking-up represents
Different significant (P < 0.05).)
Table 5: the acrosomal integrity comparison that different dilution powder dilution sperm are saved in 9 to 14 DEG C of temperature regions
(same column data in table, shoulder marking-up parent phase is not significant (P > 0.05) with difference is represented, and it is poor that the female difference of shoulder marking-up represents
Different significant (P < 0.05).)
Compared by comparative test, Examples 1 to 3, comparative example 1~2 dilution powder formula at 4~9 DEG C and 9~14
DEG C temperature range save 3 days, 5 days, 7 days after sperm, it is low that vigor, acrosomal integnity are not added with sperm than control group BTS
The protectant BTS dilution powder of temperature is high, and significant difference.Illustrate that the dilution powder for adding cryoprotective agent in the present invention has sperm
Apparent low-temperature region protecting effect, has widened the preservation and transport temperature interval width of sperm.And trehalose is added simultaneously
And soybean lecithin has collaboration to the vigor and acrosomal integrity that improve Semen routine in 4~20 DEG C of wide temperature region
Effect.
Claims (6)
1. the fresh essence of a boar saves dilution powder, it includes energy matter nutritional agents, osmotic pressure adjusting that the fresh essence of pig, which saves dilution powder,
Agent, pH adjusting agent and weak acid salt buffer agent, which is characterized in that further include cryoprotective agent, the cryoprotective agent is sea
Algae sugar and soybean lecithin.
2. the fresh essence of pig according to claim 1 saves dilution powder, it is characterised in that further include antibiotic.
3. the fresh essence of pig according to claim 1 saves dilution powder, it is characterised in that: the energy matter nutritional agents is Portugal
Grape Icing Sugar；The osmotic pressure regulator is NaCl and/or KCl；The pH adjusting agent is sodium ethylene diamine tetracetate chelating
Agent；The weak acid salt buffer agent is sodium citrate.
4. the fresh essence of pig according to claim 1 saves dilution powder, it is characterised in that: to be diluted to 1000 milliliters of dilution institutes
The fresh essence of the pig needed saves dilution powder meter,
Energy matter nutritional agents is 20.0~35.0g, the 1.0~3.0g of pH adjusting agent, the osmotic pressure regulator 0.2~
1.0g, the weak acid salt buffer agent 5.0~10.0g, the 1.0~18.0g of trehalose, the soybean lecithin 1.0~
18.0g。
5. the fresh essence of pig according to claim 4 saves dilution powder, it is characterised in that: further include antibiotic.
6. the fresh essence of pig according to claim 5 saves dilution powder, it is characterised in that: the antibiotic be penicillin 1.0~
2.0g, 1.0~2.0g of streptomysin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811113135.3A CN109221085A (en) | 2018-09-25 | 2018-09-25 | The fresh essence of pig saves dilution powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811113135.3A CN109221085A (en) | 2018-09-25 | 2018-09-25 | The fresh essence of pig saves dilution powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109221085A true CN109221085A (en) | 2019-01-18 |
Family
ID=65056604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811113135.3A Pending CN109221085A (en) | 2018-09-25 | 2018-09-25 | The fresh essence of pig saves dilution powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109221085A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115868476A (en) * | 2022-09-14 | 2023-03-31 | 广州金晨生物技术有限公司 | Long-acting boar semen dilution powder and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101611705A (en) * | 2009-07-23 | 2009-12-30 | 于文龙 | A kind of diluting boar semen powder and preparation method thereof |
| CN102726367A (en) * | 2012-06-12 | 2012-10-17 | 宁波市畜牧工程技术研究服务中心 | Low-temperature protective agent of boar semen |
| CN105660609A (en) * | 2016-04-22 | 2016-06-15 | 李志新 | Bovine seminal fluid preservation method |
| CN105794770A (en) * | 2016-04-22 | 2016-07-27 | 李志新 | Special diluent for boar semen |
| CN105794769A (en) * | 2016-04-22 | 2016-07-27 | 李志新 | Special diluting liquid for bovine seminal plasma |
| CN105815307A (en) * | 2016-04-22 | 2016-08-03 | 李志新 | Boar semen storage method |
| CN107333752A (en) * | 2017-08-08 | 2017-11-10 | 重庆信首科技有限公司 | Kind ox seminal fluid Cryopreservation |
-
2018
- 2018-09-25 CN CN201811113135.3A patent/CN109221085A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101611705A (en) * | 2009-07-23 | 2009-12-30 | 于文龙 | A kind of diluting boar semen powder and preparation method thereof |
| CN102726367A (en) * | 2012-06-12 | 2012-10-17 | 宁波市畜牧工程技术研究服务中心 | Low-temperature protective agent of boar semen |
| CN105660609A (en) * | 2016-04-22 | 2016-06-15 | 李志新 | Bovine seminal fluid preservation method |
| CN105794770A (en) * | 2016-04-22 | 2016-07-27 | 李志新 | Special diluent for boar semen |
| CN105794769A (en) * | 2016-04-22 | 2016-07-27 | 李志新 | Special diluting liquid for bovine seminal plasma |
| CN105815307A (en) * | 2016-04-22 | 2016-08-03 | 李志新 | Boar semen storage method |
| CN107333752A (en) * | 2017-08-08 | 2017-11-10 | 重庆信首科技有限公司 | Kind ox seminal fluid Cryopreservation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115868476A (en) * | 2022-09-14 | 2023-03-31 | 广州金晨生物技术有限公司 | Long-acting boar semen dilution powder and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101543208B (en) | Powder for pig sperm dilution and room temperature preservation and preparation method and application thereof | |
| CN103518706A (en) | Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe | |
| CN103548812B (en) | Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid | |
| CN102578079A (en) | Base fluid for diluting semens of equus animals and preparation method and use method thereof | |
| CN102648708B (en) | Freezing liquid for embryo or cells and application thereof | |
| CN107183014B (en) | Efficient grouper sperm cryopreservation liquid and using method thereof | |
| CN101418282A (en) | Pig bagged freezing semen preservation liquor and preservation method thereof | |
| CN102726367A (en) | Low-temperature protective agent of boar semen | |
| CN101392236A (en) | Antifreezer for diluting boar semen | |
| CN104161036A (en) | Antifreeze agent and diluent for livestock semen refrigeration preservation and application thereof | |
| CN105325401A (en) | Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof | |
| CN104322483A (en) | Boar antiviral long-acting semen diluent formula and preparation method | |
| Albiaty et al. | Effect of extenders and preservation periods in some semen characteristics of Awassi rams | |
| CN109221085A (en) | The fresh essence of pig saves dilution powder | |
| CN101611705A (en) | A kind of diluting boar semen powder and preparation method thereof | |
| CN105850978A (en) | Boar semen room-temperature preservation dilution powder and diluent, and application of dilution powder and diluent | |
| CN110463687A (en) | A kind of the preservation liquid and store method of sheep sperm | |
| CN110012901B (en) | Diluting preparation for preserving boar semen at normal temperature | |
| CN107333752A (en) | Kind ox seminal fluid Cryopreservation | |
| CN106818708B (en) | Ultralow temperature cryoprotectant for epinephelus coioides semen and preservation method thereof | |
| CN107094755A (en) | It is a kind of to improve freeze proof diluent of nutrition that sperm in vitro is preserved and preparation method thereof | |
| CN103858858A (en) | Horse semen dilution and its preparation method | |
| Ullah et al. | Enhancement of extender excellence of frozen bull semen using α-Tocopherol as an antioxidant | |
| CN106818709A (en) | Cattle freezing seminal fluid dilution preparation method | |
| CN102726371A (en) | Long-acting boar semen diluting powder and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190118 |