CN102124895B - Method for cutting propagation of cynaroides king protea - Google Patents
Method for cutting propagation of cynaroides king protea Download PDFInfo
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- CN102124895B CN102124895B CN2010106081540A CN201010608154A CN102124895B CN 102124895 B CN102124895 B CN 102124895B CN 2010106081540 A CN2010106081540 A CN 2010106081540A CN 201010608154 A CN201010608154 A CN 201010608154A CN 102124895 B CN102124895 B CN 102124895B
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- protea
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- 238000000034 method Methods 0.000 title claims abstract description 10
- 240000005063 Protea cynaroides Species 0.000 title abstract description 8
- 235000015350 Protea cynaroides Nutrition 0.000 title abstract description 5
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000008774 maternal effect Effects 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 7
- 239000003415 peat Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 9
- 241000238631 Hexapoda Species 0.000 claims description 7
- 201000010099 disease Diseases 0.000 claims description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000018044 dehydration Effects 0.000 claims description 4
- 238000006297 dehydration reaction Methods 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 230000004083 survival effect Effects 0.000 abstract description 9
- 239000000758 substrate Substances 0.000 abstract description 5
- 238000007605 air drying Methods 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 229910001562 pearlite Inorganic materials 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 238000005507 spraying Methods 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000208474 Protea Species 0.000 description 2
- 241000208465 Proteaceae Species 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 1
- 241000272165 Charadriidae Species 0.000 description 1
- 239000005781 Fludioxonil Substances 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 244000170916 Paeonia officinalis Species 0.000 description 1
- 235000006484 Paeonia officinalis Nutrition 0.000 description 1
- 241000577218 Phenes Species 0.000 description 1
- 229930182764 Polyoxin Natural products 0.000 description 1
- 241001635574 Sabatia angularis Species 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical compound C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for cutting propagation of cynaroides king protea. The method comprises the following steps of: selecting a cynaroides king protea maternal plant, scissoring a twig from a position above a semi-lignified part as a cutting slip, and keeping 4 to 6 leaves; preparing a substrate by mixing peat and pearlite according to a volume ratio of 4:6, adjusting the ph value to be between 5.5 and 6.5 and the ec value less than 0.5; soaking the basal part of the cutting slip by using 1,000 ppm indole-3-butyric acid solution, and air-drying the basal part immediately after soaking 15 minutes; controlling the cutting depth to be 1/3 to 1/4 of the length of the cutting slip; and performing management according to a conventional technique after cutting, and performing field transplantation after seedling emergence. The application of the technique ensures that the survival rate of the cutting propagation of the cynaroides king protea reaches over 90 percent, and the propagation cycle is shortened to be 40 to 60 days. The obtained seedling is good in quality and has a long service life, and the offspring performance characteristics are stable and consistent, so a technical base for selecting and cultivating a new species with excellent characteristics is established. The method is simple and convenient, and has a low cost, a high seedling survival rate and a good market application prospect.
Description
Technical field
The invention belongs to field of plant growing technology, relate to plant asexual multiplication technology, be specifically related to a kind of quick breeding king Supreme Being king blossom (
Cynaroides King Protea) cottage method.
Background technology
The emperor spend into Proteaceae (
Proteaceae) the Pu Luodi subgenus (
Protea) evergreen shrubs.Like warm, dry slightly and sunny environment, cold-resistant, the soil water of should feel well is avoided ponding, requires loose and well-drained acid ground, and winter temperature generally is not less than 2 ℃, and kind and kind can be in short-term anti--4 ℃ individually.General loti subgenus (
Protea) originate in South Africa, there are about more than 130 to plant and kind, and king Supreme Being king blossom (
Protea cynaroides
King Protea) be a wherein the most well-known kind, be the national flower in South Africa.King emperor spray leafiness, flower is big, and its flower is actual to be a bouquet, and many colored cores are arranged at the center, and is surrounded by huge, coloury bract.The bouquet diameter is at 12～30 centimetres.In 1 year, the plant that a strain is big and sturdy can be left 6～10 bouquets, and indivedual plant can be left 40 bouquets.The color of bouquet bract changes between from the milky to the peony, but rose pink, be most popular with silvery bract and praise highly slightly.In Cape province, west, South Africa, many families are arranged with plantation emperor Hua Weisheng, the emperor of local plantation spends European countries such as exporting to Holland mostly, is the local income of bringing 4,000 ten thousand dollars every year.Once in a while on New Year's Day or other festivals the time, flower market has " quick glance is pleasantly surprised " in Chinese big city in recent years, and they are mostly from Holland or Australian import, because the honorable people of making in general Lip river Supreme Being Asia are deeply aware of one's own helplessness when faced with a great task.If can make this famous flower that is loved by the people get into Chinese common people family, the market potential is very big.But because South Africa king Supreme Being king blossom output is limited, the demand of international market is very big again, and China is just in time opposite with the season in South Africa, and China with the demand of greatly alleviating the international market, and obtains abundant profit if can successfully plant when the seasonal freight source shortage.To successfully plant the emperor and spend, at first need solve the supply problem of seedling.
King Supreme Being king blossom (
Protea cynaroides
King Protea) adopt seed propagation usually, but that the seedling fecund changes is different, causes good proterties to can not get keeping, and the homogeneity of product of producing is poor, has restricted scale commodity production, is unfavorable for market sale.Simultaneously the import seed costs an arm and a leg, and the insemination and emergence rate is low, has only about 40%, and takes more than 3 years and just can bloom, and causes production cost higher.The technology of nourishing and generating such as tissue culture and cuttage is studied both at home and abroad always and is tested, and does not still have the example of success at present.And adopt the survival rate of conventional cottage propagation technology extremely low, have only about 10%, and required rootage duration reaches 1 year.How to improve king Supreme Being king blossom cuttage and seedling culture survival rate, shorten growing-seedling period, from fine individual plant breeding commodity seedling, solve king Supreme Being king blossom type indefinite (from narrow funnel type to the square position type), color indefinite (from vain to pale red), the difficult problem at florescence indefinite (from the spring to the winter) just become problem demanding prompt solution the prior art.
Summary of the invention
The object of the invention is the deficiency to prior art, provides a kind of survival rate high, king Supreme Being king blossom cuttage breeding method with low cost, simple to operate.
The object of the invention is achieved through following technical scheme.
Except as otherwise noted, the percentage that the present invention adopted is mass percent.
The cuttage breeding method of a kind of king Supreme Being king blossom may further comprise the steps:
(1) prepare in the seedbed: matrix adopts peat and perlite by volume for 4:6 mixes, adjustment pH value 5.5～6.5, and the EC value is less than 0.5, and the liquor natrii hypochloritis who uses 500ppm is to seedling-cultivating tray, seedbed and greenhouse ground sterilization;
(2) maternal plant is selected: 1～3 year seedling age healthy plant gathering no damage by disease and insect requires the branches and leaves dry tack free as maternal material, prevents the material dehydration simultaneously;
(3) inserting ear selection selects and put in order: select the ripe young sprout branch of blade for transplanting a cutting, require simultaneously that the branch terminal bud is full to be expanded and do not have rudiment, the branch diameter is at 0.4～1.0cm; Cutting is cut into the long slotting fringe of 8～12 cm, keeps 4～6 pieces of blades in top, all the other removals;
(4) inserting fringe handles: use the indole-3-butyric acid solution of 1000ppm to soak slotting fringe base portion, soaking depth is 1/2～1/3 of a cutting length, soak time 15 minutes; Air-dry immediately after soaking well; Wherein, indole-3-butyric acid needs to use 75% alcohol to be dissolved into the solution that concentration is 6000～10000ppm earlier, adds the solution that pure water is converted into 1000ppm again;
(5) cuttage: the cuttage degree of depth is 1/2～1/3 of a cutting length;
(6) manage after the cuttage: adopt routine techniques to carry out temperature treatment after the cuttage, illumination management, moisture management, the water management and the extermination of disease and insect pest, treating that skewer fringe root is tried to get to the heart of a matter from seedling carries out field-transplanting after growing.
The present invention has following advantage with respect to prior art:
1, the utilization of this technology reaches more than 90% the survival rate of king Supreme Being king blossom cottage propagation, and the breeding cycle foreshortens to 40～60 days.The king emperor seeds of flowering plants seedling that obtains is best in quality, and the seedling life-span is long, offspring's phene stable and consistent, and field planting promptly began to bloom in 1 year, made the plantation of king Supreme Being king blossom scale commodity become possibility.
2, the inventive method is easy, with low cost, the kind shoot survival percent is high, has market application foreground well.Just can bloom by 3 years of tree seedling and to shorten to 1～2 year.
3. the success of king Supreme Being king blossom cottage propagation for selecting merit, is cultivated the outstanding new varieties of king Supreme Being king blossom and has been established technical foundation.
Embodiment
Below in conjunction with embodiment the present invention is done further detailed description, but embodiment is not the qualification to technical scheme of the present invention.
Embodiment 1
In the Plastic film greenhouse that is coated with 60% black sunshade net, set up through the built on stilts seedbed of liquor natrii hypochloritis's sterilization of 500ppm, on the seedbed, lay the seedling-cultivating tray that has filled matrix.Matrix is filled in the seedling-cultivating tray in 32 holes after using peat and perlite to mix as 4:6 by volume, shakes one gently and to secondary, upper surface is wipeed off.Start spraying system, make water content of substrate saturated.Regulate substrate pH value 6.0, EC value 0.4.
Select seedling age 1～3 year, no damage by disease and insect, eugonic king Supreme Being king blossom (
Protea cynaroides
King Protea) as maternal, collection will not carried out when fine day has mist and do not have dew, guaranteeing the branches and leaves dry tack free, and prevents the material dehydration.
The full young sprout branch that expands and do not have rudiment while blade maturation of the terminal bud that clip grows is recently transplanted a cutting diameter in 0.4～1.0cm scope as cutting.Then cutting is cut into the slotting fringe of length 8～12cm.
Workbench is noted preserving moisture, and removes and inserts the unnecessary blade of fringe base portion, and each slotting fringe keeps 4～6 pieces of blades, inserts fringe and pricks with 32, the base portion alignment.Scissors is sharp, and per 10 minutes with 75% wipes of alcohol wash disinfection.Indole-3-butyric acid solution with 1000ppm soaked slotting fringe base portion 15 minutes, carried out HORMONE TREATMENT.Depth of immersion is 1/2～1/3 of a cutting length.Air-dry immediately after soaking well.Wherein, the purity of hormone indole-3-butyric acid is 90%, uses 75% alcohol to be dissolved into the solution of concentration as 10000ppm earlier, adds the solution that pure water is converted into 1000ppm again.
Cuttage in filling in 32 hole seedling-cultivating trays of matrix, one strain of every hole, the cuttage degree of depth is a cutting length 1/2～1/3.
Management after the cuttage: keep relative air humidity in 70%～100% scope.Air humidity and water supply in media management are to adopt the automatic intermittent spraying method.Use the evaporation coefficient automatic control mode.For avoiding the appearance of error, fine day twice spray time at noon 20 minutes at interval are set.In the 1st～6 week after the cuttage (before taking root), the fine 9:00 of spraying system opens, and 18:00 closes, and overcast and rainy 11:00 opens, and 15:00 closes; 30 seconds duration of spraying, prevent the dehydration of earing; The 7th～8 week reduced moisture content gradually, reduced water content of substrate, and fine 9:30 opens, and 17:30 closes, and overcast and rainy 12:00 opens, and 14:30 closes 20 seconds duration of spraying.In the 9th～10 week, further reduce water content of substrate, to promote root growth.Fine 10:00 opens, and 17:00 closes, and overcast and rainy 12:00 opens, and 14:00 closes; 15 seconds duration of spraying.The 11st week got into practices seedling stage, and 12:00 opens, and 14:00 closes and keeps matrix moistening, avoids dry; When matrix is dry, take the circumstances into consideration spraying continuously.Illumination and temperature treatment: the control day temperature is below 30 ℃, and night, temperature was at 14～20 ℃.Light intensity is at 10～20KLX, periodicity of illumination 10～16 hours.Improve intensity of illumination after taking root gradually.The extermination of disease and insect pest: adopt 1000 times of liquid of the bright ammonium of 25% miaow, 1500 times of liquid of 2.5% fludioxonil, 1000 times of liquid of 50% thiophanate-methyl and the alternately spraying of 1000 times of liquid of 10% polyoxin, 2 times/week, close spraying afternoon and carry out afterwards.It is dry and comfortable to keep insert the fringe blade night.
Fringe root to be inserted is tried to get to the heart of a matter from seedling and is transplanted to the land for growing field crops when growing, shallow planting, and other are identical with conventional field planting.
Statistics finds, king Supreme Being king blossom began to have new root to grow in 40 days after cuttage, can carry out field-transplanting when survival rate reaches 95%, 60 day.Improve than existing normal cutting propagation propagation technique survival rate nearly 60%, 9～10 months in advance field-transplanting time.The cuttage seeding well developed root system, no damage by disease and insect, seedling reaches one-level seedling quality standard generally.Field planting can be bloomed (1/strain) in 1 year, 2 annuals, 3～5 of the every strains of blooming.Flowering time has shifted to an earlier date 2 years than seedling.Simultaneously, the colored type that cuttage seeding is left, pattern, florescence and former maternal plant basically identical, this has established technical foundation for realizing scale commodity production and seed selection improved seeds.
Embodiment 2
Repeat embodiment 1, following difference is arranged: the pH value of adjustment matrix is 5.5.Indole-3-butyric acid needs to use 75% alcohol to be dissolved into the solution of concentration as 8000ppm earlier, adds the solution that pure water is converted into 1000ppm again.
Embodiment 3
Repeat embodiment 1, following difference is arranged: the pH value of adjustment matrix is 6.5.Indole-3-butyric acid needs to use 75% alcohol to be dissolved into the solution of concentration as 6000ppm earlier, adds the solution that pure water is converted into 1000ppm again.
Claims (1)
1. the cuttage breeding method of a king Supreme Being king blossom may further comprise the steps:
(1) prepare in the seedbed: matrix adopts peat and perlite by volume for 4:6 mixes, adjustment pH value 5.5～6.5, and the EC value is less than 0.5, and the liquor natrii hypochloritis who uses 500ppm is to seedling-cultivating tray, seedbed and greenhouse ground sterilization;
(2) maternal plant is selected: the healthy plant of 1～3 year seedling age of the no damage by disease and insect of collection requires the branches and leaves dry tack free as maternal material, prevents the material dehydration simultaneously;
(3) inserting ear selection selects and put in order: select the ripe young sprout branch of blade for transplanting a cutting, require simultaneously that the branch terminal bud is full to be expanded and do not have rudiment, the branch diameter is at 0.4～1.0cm; Cutting is cut into the long slotting fringe of 8～12 cm, keeps 4～6 pieces of blades in top, all the other removals;
(4) inserting fringe handles: use the indole-3-butyric acid solution of 1000ppm to soak slotting fringe base portion, soaking depth is 1/2～1/3 of a cutting length, soak time 15 minutes; Air-dry immediately after soaking well; Wherein, indole-3-butyric acid needs to use 75% alcohol to be dissolved into the solution that concentration is 6000～10000ppm earlier, adds the solution that pure water is converted into 1000ppm again;
(5) cuttage: the cuttage degree of depth is 1/2～1/3 of a cutting length;
(6) manage after the cuttage: adopt routine techniques to carry out temperature treatment after the cuttage, illumination management, moisture management, the water management and the extermination of disease and insect pest, treating that skewer fringe root is tried to get to the heart of a matter from seedling carries out field-transplanting after growing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106081540A CN102124895B (en) | 2010-12-28 | 2010-12-28 | Method for cutting propagation of cynaroides king protea |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106081540A CN102124895B (en) | 2010-12-28 | 2010-12-28 | Method for cutting propagation of cynaroides king protea |
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| Publication Number | Publication Date |
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| CN102124895A CN102124895A (en) | 2011-07-20 |
| CN102124895B true CN102124895B (en) | 2012-02-22 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103548698A (en) * | 2013-11-08 | 2014-02-05 | 上海孙桥农业科技股份有限公司 | Method for quickly propagating protea cynaroides |
| CN104429627A (en) * | 2014-10-14 | 2015-03-25 | 深圳文科园林股份有限公司 | Huodendrontibeticumcuttage method |
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Application publication date: 20110720 Assignee: Kunming Yang Yueji Horticulture Co., Ltd. Assignor: Yang Yuyong Contract record no.: 2014530000097 Denomination of invention: Method for cutting propagation of cynaroides king protea Granted publication date: 20120222 License type: Exclusive License Record date: 20140722 |
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Application publication date: 20110720 Assignee: Kunming Yang Yueji Horticulture Co., Ltd. Assignor: Yang Yuyong Contract record no.: 2015530000068 Denomination of invention: Method for cutting propagation of cynaroides king protea Granted publication date: 20120222 License type: Common License Record date: 20150910 |
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Effective date of registration: 20190719 Address after: 650500 Maitianwan Peach Garden, Beidou Village, Yangzong Town, Yangzonghai Scenic Area, Kunming City, Yunnan Province Patentee after: Kunming southern flowers horticulture science and technology limited liability company Address before: 650500 Yunnan Kunming Majinpu Township Zhongwei Village Yang Rose Horticulture Co., Ltd. Patentee before: Yang Yuyong |
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