CN102124840A - Method for promoting germination of strawberry seeds - Google Patents
Method for promoting germination of strawberry seeds Download PDFInfo
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- CN102124840A CN102124840A CN 201110039179 CN201110039179A CN102124840A CN 102124840 A CN102124840 A CN 102124840A CN 201110039179 CN201110039179 CN 201110039179 CN 201110039179 A CN201110039179 A CN 201110039179A CN 102124840 A CN102124840 A CN 102124840A
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Abstract
The invention provides a method for promoting germination of strawberry seeds, belonging to the strawberry breeding technique. In the prior art, the rate of emergence of strawberry seeds is low, emergent seedlings are irregular, the labor and time are wasted in seedling management, and fine genetic genes of 60-70% of ungerminated seeds are lost. The technical scheme provided by the invention comprises the following steps of: (A) drying pretreatment: insolating in the sun, or drying at low temperature; (B) high-temperature treatment: treating seeds for 24+/-1 hours at the constant temperature of 30+/-2 DEG C, treating the seeds for 24+/-1 hours at the constant temperature of 35+/-2 DEG C, and treating the seeds for 120+/-5 hours at the constant temperature of 40+/-2 DEG C; (C) low-temperature treatment: treating the seeds for 168+/-5 hours at the constant temperature of 0+/-1 DEG C, and then, drying the seeds; and (D) pickling treatment: immersing the seeds into sulfuric acid the concentration of which is 98% for 60+/-5 seconds, repeatedly washing the seeds with clear water, and then, airing the seeds for seeding. The invention shows the following effects: the rate of emergence is increased from 15-25% to 80-85% within 40 days; the seedling emergence regularity reaches more than 85% after 40 days; and 42-50% of seeds which do not germinate after being originally seeded for 60 days are bred.
Description
Technical field
The invention belongs to the strawberry breeding technique, particularly a kind of method that promotes the strawberry seed germination.
Background technology
The strawberry plant is the height heterozygote, and in the colony of growing directly from seeds, each individual plant all is different from other plant on genetic character.Therefore, the appearance of the disease-resistant strawberry new varieties of high-quality, its main method is the means by the conventional hybridization breeding, the offspring carries out individual plant selection with the hybrid seed gained.So the germination rate of hybrid seed and the regularity of emerging become the key link in the strawberry breeding.According to " Jiangsu agricultural journal " the 2nd phase of calendar year 2001, Ma Hongxiang, Yu Guihong, Wu Weimin, Chen Xiulan etc. report in the article of " strawberry achene cultured in vitro is to the influence of percentage of seedgermination ", the natural germination rate of strawberry seed is generally at 30-40%, not only emergence rate is low, and it is irregular to emerge, have after planting 10 days just germinate, have then needed 40 days even reach 90 days ability germinateed.This is comprised that the seedling management of transplanting seedlings has increased difficulty, not only take a lot of work but also consuming time, and 60-70% seeds germinated not wherein, good genetic losing largely caused, reduce the probability of breeding of new variety success, seriously perplexed the progress of strawberry scale crossbreeding work.
This shows that study and design a kind of seed germination rate and regularity of emerging of increasing substantially, the method that can increase the promotion strawberry seed germination of breeding of new variety success rate again is necessary.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, study and design a kind of seed germination rate and regularity of emerging of increasing substantially, can increase the method for the promotion strawberry seed germination of breeding of new variety success rate again.
Purpose purpose of the present invention is achieved in that a kind of method that promotes the strawberry seed germination, it is characterized in that before conventional seed planting is grown seedlings, and high temperature---low temperature---pickling of seed being carried out be made up of following steps is handled:
A, dry preliminary treatment: the strawberry seed placed tan by the sun under the sunlight or low temperature drying below 30 ℃, it is standby to make seed moisture content reach 12～14% backs;
B, high temperature treatment: the pretreated seed of drying is placed in the vessel, put into insulating box or drying box, implement temperature step-by-step processing process from low to high: the first step was handled 24 ± 1 hours at 30 ± 2 ℃ of constant temperature earlier, second step handled 24 ± 1 hours at 35 ± 2 ℃ of constant temperature again, last the 3rd step handled 120 ± 5 hours at 40 ± 2 ℃ of constant temperature, and the cooling of giving free rein to is then reduced to after the room temperature standby;
C, low temperature treatment: the seed immigration after high temperature treatment is covered with covering in the vessel of blotting paper, use sprayer seed to be squirted with distilled water, moistening with the surface of the seed, do not see open fire degree of appearing as, add the vessel lid, place 0 ± 1 ℃ of constant temperature in cold compartment of refrigerator inherence to handle after 168 ± 5 hours then, place and tan by the sun under the sunlight or low temperature drying below 30 ℃, it is standby to make seed moisture content reach 12～14% backs;
D, pickling handle: the seed after low temperature treatment being moved in the vessel, pour concentrated acid into, is 98% sulfuric acid as concentration, all immerses degree of being in the acid with seed, evenly slightly rocks vessel constantly and lasts for 60 ± 5 seconds; Promptly the seed of handling well is poured in the nylon mesh funnel that is immersed in the clear water then, washed repeatedly with clear water again,, use through drying again for seeding and seedling raising so that float concentrated acid on the seed.
The mesh aperture of described nylon mesh funnel is less than the minimum grain size of strawberry seed.
Technical scheme of the present invention is compared with the common seed that---low temperature---pickling without high temperature is handled through implementing, and has shown following beneficial effect in the seeding and seedling raising phase:
1, improves seed germination rate significantly.Percentage of seedgermination is in after planting 40 days, and the common percentage of seedgermination 15-25% that is handled by---low temperature---pickling without high temperature brings up to 80-85%.The common percentage of seedgermination that---low temperature---pickling was handled without high temperature in after planting 60 days still is difficult to reach 40%, and the percentage of seedgermination after the invention process stably surpasses 85%.
2, improved the regularity of emerging of seed significantly.The common percentage of seedgermination that---low temperature---pickling is handled without high temperature reached 15-25% in after planting 40 days, again through reaching 35-38% in 20 days successively; And the percentage of seedgermination after the invention process promptly reaches 80-85% in 40 days, just stable later on reaching more than 85%, even still be stabilized in 85-87%. through 20 days percentages of seedgermination again, that is to say, need only the regularity of emerging that just can reach more than 85% through 40 days.
3, increased the seed selection success rate of new varieties.As previously mentioned, just can stably reach more than 85% after planting 40 days through the percentage of seedgermination after the invention process, and the common percentage of seedgermination that---low temperature---pickling is handled without high temperature just can reach 35-38% to after planting 60 days, both shifted to an earlier date breeding process, make the seed of the 42-50% that the script sowing that may have the good advantage gene also do not germinate in 60 days obtain the chance of seed selection again, must improve the seed selection success rate of new varieties.
Embodiment
Since two thousand seven, the applicant has born a plurality of strawberry breeding of new variety research projects that Jiangsu Province Science and Technology Department assigns, and the low and irregular problem of emerging of strawberry percentage of seedgermination had once once perplexed the progress of scale crossbreeding work.For this reason, adopt the high temperature and the low temperature of natural imitation circle, seed is carried out alternating temperature handle, and then seed is handled, particularly carry out pickling and handle, be intended to eliminate strawberry seed epidermis wax that has and the thickness that reduces hard epidermis with different medicaments.Through test of many times, obtained a kind of seed germination rate and regularity of emerging of increasing substantially of the present invention finally, can increase the method for the promotion strawberry seed germination of breeding of new variety success rate again.
Now specific embodiments is described as follows:
The seed of common cultivar " bright treasured " is selected in this test for use, designs 12 processing, and each is handled 3 times and repeats, and repeats 300 seeds at every turn.Processing is respectively: (1) is only through high temperature treatment; (2) only through low temperature treatment; (3) high temperature and low temperature treatment, promptly alternating temperature is handled; (4) no any processing compares group; (5)～(9) after handling through alternating temperature earlier, use 10 times of liquid of the concentrated sulfuric acid of concentration 5ppm, 10ppm, 20ppm gibberellin liquid and concentration 98%, 100 times of liquid again, handled respectively 3 hours; (10)～(12) same earlier through after the alternating temperature processing, directly handled 1 minute, 2 minutes, 3 minutes with the concentrated sulfuric acid of concentration 98%.Handle to clean and to put into the constant temperature illumination box after finishing, temperature is arranged on 25 ℃, and light application time is that 8 of mornings are to point in evenings 8.Instituted an inquiry germination rate in back 10 days from handling, investigation in per afterwards 2～3 days is once sorted out seeds germinated at every turn and is rejected and keep a record, continuous observation to 60 day.The test data of investigation sees Table 1.
Table 1, different disposal are to the influence of percentage of seedgermination
By the experimental data that obtains in the table 1, analyze and draw following result:
1, in 11 kinds of different processing and 1 group are untreated, can draw from listed germination rate experimental data contrast: in the time of 40 days, " (10) alternating temperature+the immersion concentrated sulfuric acid 1 minute " is that the germination rate of A-D step seed treatment of the present invention is the highest, reaches 85.6%; And " (4) untreated control group " germination rate only has 18.2%.
2, from the listed germination rate experimental data of (10) row, can draw: " (10) alternating temperature+the immersion concentrated sulfuric acid 1 minute " after 40 days, the germination rate up to 60 days is stabilized in 85.6%, and just after 40 days, germination rate only is not 14.4%.
3, from the listed germination rate experimental data of (4) row, can draw: " (4) untreated control group " germination rate ", after 40 days, still constantly germinate successively, also only be 38.4% up to 60 days germination rates, just germination rate is not finished up to 61.6%.
4, obtain from the listed germination rate experimental data contrast of (1)-(3) row: the high temperature-low temperature treatment described in the present invention is rationally and necessity.
5, obtain from the listed germination rate experimental data contrast of (5)-(10) row: though the germination rate of the immersion gibberellin treatment handled of pickling is not relatively low for high temperature-low temperature treatment, it is rationally and necessary that the high temperature-low temperature described in the present invention-pickling is handled.
6, obtain from the listed germination rate experimental data contrast of (8)-(12) row: adopting the acid of high concentration to carry out the processing of short time during the high temperature-low temperature described in the present invention-pickling is handled is optimal selection.
More than be the specific embodiment of the present invention, but do not constitute restriction the scope of claim protection of the present invention.
Subject technology design of the present invention is high temperature and the low temperature that adopts natural imitation circle, seed is carried out alternating temperature to be handled, and then with different medicaments seed is handled, particularly carry out pickling and handle, be intended to eliminate wax that strawberry seed epidermis has and the thickness that reduces hard epidermis.The key of claim of the present invention protection is that high temperature-low temperature-pickling is handled, and when wherein pickling is handled, and adopting the acid of high concentration to carry out the processing of short time is optimal selection.Every improvement or equivalent transformation of doing based on above-mentioned technical conceive of the present invention; selection as the temperature and time in high temperature-low temperature treatment; during handling, pickling selects sulfuric acid, nitric acid, hydrochloric acid or its combination for use for reaching elimination strawberry seed epidermis wax that has and the thickness that reduces hard epidermis; thereby when bringing the short time to handle; the selections of time length etc. all fall into the scope that claim of the present invention is protected.
Claims (2)
1. a method that promotes the strawberry seed germination is characterized in that before conventional seed planting is grown seedlings, and high temperature---low temperature---pickling of seed being carried out be made up of following steps is handled:
A, dry preliminary treatment: the strawberry seed placed tan by the sun under the sunlight or low temperature drying below 30 ℃, it is standby to make seed moisture content reach 12～14% backs;
B, high temperature treatment: the pretreated seed of drying is placed in the vessel, put into insulating box or drying box, implement temperature step-by-step processing process from low to high: the first step was handled 24 ± 1 hours at 30 ± 2 ℃ of constant temperature earlier, second step handled 24 ± 1 hours at 35 ± 2 ℃ of constant temperature again, last the 3rd step handled 120 ± 5 hours at 40 ± 2 ℃ of constant temperature, and the cooling of giving free rein to is then reduced to after the room temperature standby;
C, low temperature treatment: the seed immigration after high temperature treatment is covered with covering in the vessel of blotting paper, use sprayer seed to be squirted with distilled water, moistening with the surface of the seed, do not see open fire degree of appearing as, add the vessel lid, place 0 ± 1 ℃ of constant temperature in cold compartment of refrigerator inherence to handle after 168 ± 5 hours then, place and tan by the sun under the sunlight or low temperature drying below 30 ℃, it is standby to make seed moisture content reach 12～14% backs;
D, pickling handle: the seed after low temperature treatment being moved in the vessel, pour concentrated acid into, is 98% sulfuric acid as concentration, all immerses degree of being in the acid with seed, evenly slightly rocks vessel constantly and lasts for 60 ± 5 seconds; Promptly the seed of handling well is poured in the nylon mesh funnel that is immersed in the clear water then, washed repeatedly with clear water again,, use through drying again for seeding and seedling raising so that float concentrated acid on the seed.
2. a kind of method that promotes the strawberry seed germination according to claim 1 is characterized in that the minimum grain size of the mesh aperture of described nylon mesh funnel less than the strawberry seed.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444303A (en) * | 2012-05-31 | 2013-12-18 | 苏丽娟 | Processing method for improving germination rate of strawberry seeds |
| CN103733759A (en) * | 2013-12-23 | 2014-04-23 | 林雪桃 | Strawberry seed pregermination method |
| CN104593410A (en) * | 2015-01-05 | 2015-05-06 | 北京农学院 | Agrobacterium rhizogenes mediated strawberry gene transferring method |
| CN105191633A (en) * | 2015-09-29 | 2015-12-30 | 淮北市四季蔬菜种植有限公司 | Seedling culturing method capable of raising emergence rate of strawberry seeds |
| CN106613238A (en) * | 2016-12-23 | 2017-05-10 | 庆阳敦博科技发展有限公司 | Dioscorea zingiberensis cultivation method in high attitude area |
| CN110583139A (en) * | 2018-06-13 | 2019-12-20 | 郭睿 | Method for improving germination rate of strawberry seeds |
| CN112385530A (en) * | 2020-11-17 | 2021-02-23 | 云南省农业科学院花卉研究所 | Method for increasing seedling rate of space mutation strawberry seeds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101278611A (en) * | 2008-05-06 | 2008-10-08 | 江苏丘陵地区镇江农业科学研究所 | Method for treating greenhouse strawberry continuous cropping field soil |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101278611A (en) * | 2008-05-06 | 2008-10-08 | 江苏丘陵地区镇江农业科学研究所 | Method for treating greenhouse strawberry continuous cropping field soil |
Non-Patent Citations (3)
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| 《农技服务》 20081231 李金凤等 日本三重县草莓促成栽培育苗技术 123 1-2 第25卷, 第9期 2 * |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444303A (en) * | 2012-05-31 | 2013-12-18 | 苏丽娟 | Processing method for improving germination rate of strawberry seeds |
| CN103444303B (en) * | 2012-05-31 | 2015-03-18 | 成县兴丰农林科技有限责任公司 | Processing method for improving germination rate of strawberry seeds |
| CN103733759A (en) * | 2013-12-23 | 2014-04-23 | 林雪桃 | Strawberry seed pregermination method |
| CN104593410A (en) * | 2015-01-05 | 2015-05-06 | 北京农学院 | Agrobacterium rhizogenes mediated strawberry gene transferring method |
| CN105191633A (en) * | 2015-09-29 | 2015-12-30 | 淮北市四季蔬菜种植有限公司 | Seedling culturing method capable of raising emergence rate of strawberry seeds |
| CN106613238A (en) * | 2016-12-23 | 2017-05-10 | 庆阳敦博科技发展有限公司 | Dioscorea zingiberensis cultivation method in high attitude area |
| CN106613238B (en) * | 2016-12-23 | 2020-10-02 | 诸暨市物昇农业科技有限公司 | Method for cultivating yellow ginger in high-altitude areas |
| CN110583139A (en) * | 2018-06-13 | 2019-12-20 | 郭睿 | Method for improving germination rate of strawberry seeds |
| CN112385530A (en) * | 2020-11-17 | 2021-02-23 | 云南省农业科学院花卉研究所 | Method for increasing seedling rate of space mutation strawberry seeds |
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