CN102119633A - Method for improving stock grafting through scion - Google Patents
Method for improving stock grafting through scion Download PDFInfo
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- CN102119633A CN102119633A CN2010106002267A CN201010600226A CN102119633A CN 102119633 A CN102119633 A CN 102119633A CN 2010106002267 A CN2010106002267 A CN 2010106002267A CN 201010600226 A CN201010600226 A CN 201010600226A CN 102119633 A CN102119633 A CN 102119633A
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Abstract
The invention relates to a method for improving stock grafting through scion and belongs to the technical field of biological engineering. The method comprises the following steps: 1) cultivating a stock: choosing a robust seedling of a plant having subterraneous stems, tubers, and roots as the stock; 2) scion: choosing an excellent foliage branch of the same crop as scion; and 3) grafting. By using the method for improving stock grafting, the mutual effect of scion and stock is utilized, thereby taking full advantages of the stock that the seedling life is long, the root grows well, the capability of absorbing water and fertilizer is strong, the stress resistance is strong, the cultivation management is simple, the growing power is high, the seedling is easily propagated, and the like. The advantages of scion photosynthesis and secondary metabolite composition are fully taken; the quality and output of subterraneous stems, tubers, and roots of the improved stock can be enhanced by scion plant, thereby being high in application value for increasing the output and quality of the root, stem or tuber plant crops. The method for improving stock grafting can be conveniently operated and has the advantages of low cost, high efficiency and stability, accuracy, high speed, and the like.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to the grafting improved method of a kind of scion stock.
Background technology
Thunder godvine is the root of Celastraceae plant thunder godvine, cries herba fibraureae recisae, Huang Lateng, cabbageworm medicine, red medicine, water thick grass again, main product in Fujian, ground such as Zhejiang, Anhui, Henan.Former plant is born in shady and cool hillside, the forest clump or the small stream limit, should be in slant acidity, and sandy soil fertile, that soil layer is deep or yellow soil cultivation.The general cottage propagation that adopts of cultivation, fall leaves the back to early Febuary in next year dormancy in earlier stage thunder godvine, choose the branch that 1-2 gives birth to, be cut into the segment of 10-20cm, every section cutting should have 2-3 joint, to transplant a cutting then about about 2cm place, lower end immersion 100 * 10-6 methyl (NAA) solution 1h, just can be by distance between rows and hills 10cm * 10-15cm cuttage, to transplant a cutting sway in ditch with 60 ℃ of angles after, earthing compresses, basseting and partly be about the 1/4-1/3 of cutting in the upper end, waters immediately after inserting, and take the cool canopy covering; Behind the 40d, cutting can be germinateed and be taken root; Can transplant after 1 year.Thunder godvine bitter, suffering, cool in nature, big poison; Return liver, kidney channel; Effect is dispelled rheumatism, is removed obstruction in channels to relieve pain, swelling and pain relieving, detoxifcation desinsection; Be used for the long-pending poison of damp and hot tubercle, cancer knurl, treat lepra reaction, rheumatoid arthritis etc. with it clinically, pharmacological research shows that also it has effects such as antitumor, anti-inflammatory.Can be used as medicine after the root peeling of thunder godvine, through chemical examination research, it contains chemical compositions such as multiple alkaloid, lignin, amino acid.
The bark of official magnolia belongs to Magnoliaceae, nature and flavor hardship, suffering, warm in nature.Like warm cool humid climate and well-drained acid ground, be born in the shaw on hillside the foot of a mountain and small stream limit, roadside, mainly be distributed in ground such as Shaanxi, Gansu, Zhejiang, Anhui, Jiangxi, Fujian, Hubei, Hunan, Sichuan, Guizhou, cultivate in some area now.The bark of official magnolia field planting peeling that can chop at a tree more than 20 years should be carried out in peak of growing season in 4～August, a Gen Pi and a branch skin directly dry in the shade or reel after dry, claim root Piao and branch Piao.Main active ingredient in the bark of official magnolia is magnolol and honokiol, and its effect mainly contains pharmacologically actives such as antibiotic, calm nervous centralis, of flaccid muscles, antiulcer.The bark of official magnolia seed, press strip and cottage propagation.
A large amount of stocks that studies show that all have certain influence to grafting affinity, fruiting period, quality quality, output height, tree body size, adaptability and the resistance of farmings such as melon and fruit, woods, medicinal plant, but relevant scion is reported seldom the effect study of stock, utilizes report not see especially to it.
Summary of the invention
At the above-mentioned problems in the prior art, the objective of the invention is to design provides the technical scheme of a kind of scion to the grafting improved method of stock, utilize the mutual effect of scion and stock, reach subterranean stem, the stem tuber of improvement stock, the quality and the output of root system with the scion plant, have great application value.
Described a kind of scion is characterized in that may further comprise the steps to the grafting improved method of stock:
1) stock is cultivated: selecting has the seedling of subterranean stem, stem tuber, root system crop to make stock, rugosity is advisable with 1.0-2.5cm, age of tree 1-2,2-4 begins to cultivate before grafting, nursery lot is selected loosing soil, is rich in the sandy soil of organic matter, middle fertility, applied 30-50 kilogram fertilizer in 10-20 days before cultivating and make base fertilizer, plowed soils is to stock stalwartness, standby when neat;
2) scion: select crop quality of the same race, vegetative shoot that kind is good to do scion;
3) grafting: operating personnel's finger, blade, bamboo let are smeared sterilization with the 70-80% medicinal alcohol, sterilization in 1.5-2.5 hour once at interval, the stock cultivated is cut off at place at 9-12cm overhead, and stock is removed growing point and lobus cardiacus, the breach of growing at two cotyledon intermediate vertical down-cutting 1.0-2.0cm; About 2-4cm place is whittled into it in young stem both sides the two-sided wedge shape of 0.5-0.9cm length under the scion cotyledon with blade; The scion folding wedge is inserted gently in the face of accurate stock interface, scion and stock cambium are aimed at, otch is fitted tightly, fix with the sterilization Grafting clip again after 5-8 hour with 150-250 times of formalin bubble, earlier stock and scion are tightened with plastic film at last, then sealing is tightened in scion and stock interface, promptly finish grafting work.
Described a kind of scion is characterized in that to the grafting improved method of stock described stock is healthy and strong thunder godvine seedling or bark of official magnolia seedling; Described scion is good thunder godvine vegetative shoot of quality, kind or bark of official magnolia vegetative shoot.
Described a kind of scion is characterized in that selecting the seedling of the osculant bark of official magnolia to make stock to the grafting improved method of stock, selects little spur type bark of official magnolia quality, vegetative shoot that kind is good to do scion.
Described a kind of scion is characterized in that selecting the thunder godvine of Cangnan, Zhejiang to make stock to the grafting improved method of stock, and the thunder godvine intermediate form vegetative shoot of Suichang, Zhejiang is done scion.
Above-mentioned a kind of scion utilizes the mutual effect of scion and stock to the grafting improved method of stock, and it is long to give full play to the stock nursery stock life-span, root system development is good, and it is strong to absorb the liquid manure ability, strong stress resistance (especially anti-rhizosphere damage by disease and insect), cultivation management is simple, and growth potential is strong, advantages such as easy breeding; Performance scion photosynthesis, the advantage that secondary metabolite is synthetic; Reach subterranean stem, the stem tuber of improvement stock, the quality and the output of root system with the scion plant, have great application value for improving root, stem or tuber crops output and quality.Above-mentioned grafting improved method has easy to operate, with low cost, stability and high efficiency, accurate advantage such as quick.
The percentage composition that relates in the present specification unless otherwise indicated, other the weight percentage that is pure material.
Description of drawings
Fig. 1 is an osculant bark of official magnolia HPLC finger-print, and Fig. 2 is the HPLC finger-print after the little spur type bark of official magnolia in the grafting of the osculant bark of official magnolia; Among the figure: A is the honokiol mass fraction, and B is the magnolol mass fraction.
Embodiment
The present invention is further illustrated with comparative trial in conjunction with specific embodiments more below.
Embodiment 1
1) stock is cultivated: select the seedling of the osculant bark of official magnolia to make stock, rugosity is advisable with 1.0-2.5cm, age of tree 1-2,2-4 begins to cultivate before grafting, nursery lot is selected loosing soil, is rich in the sandy soil of organic matter, middle fertility, applied 30-50 kilogram fertilizer in 10-20 days before cultivating and make base fertilizer, plowed soils is to stock stalwartness, standby when neat;
2) scion: selection quality, the little spur type bark of official magnolia vegetative shoot that kind is good are done scion;
3) grafting: operating personnel's finger, blade, bamboo let are smeared sterilization with the 70-80% medicinal alcohol, sterilization in 1.5-2.5 hour once at interval, the stock cultivated is cut off at place at 9-12cm overhead, and stock is removed growing point and lobus cardiacus, the breach of growing at two cotyledon intermediate vertical down-cutting 1.0-2.0cm; About 2-4cm place is whittled into it in young stem both sides the two-sided wedge shape of 0.5-0.9cm length under the scion cotyledon with blade; The scion folding wedge is inserted gently in the face of accurate stock interface, scion and stock cambium are aimed at, otch is fitted tightly, fix with the sterilization Grafting clip again after 5-8 hour with 150-250 times of formalin bubble, earlier stock and scion are tightened with plastic film at last, then sealing is tightened in scion and stock interface, promptly finish grafting work.
The inventor is that stock, the little spur type bark of official magnolia are that scion is found when setting up bark of official magnolia germ plasm resource garden with the osculant bark of official magnolia: to osculant bark of official magnolia stock, there are remarkable influence in bark of official magnolia phenols material mass and mass fraction with the scion of the little spur type bark of official magnolia.Shown in Fig. 1-2: before the grafting, the magnolol mass fraction of the osculant bark of official magnolia (B composition in the HPLC finger-print) all is lower than the little spur type bark of official magnolia with honokiol mass fraction (A composition in the HPLC finger-print); After the grafting, the little spur type bark of official magnolia (scion) obtains bark of official magnolia new varieties in the osculant bark of official magnolia (stock) grafting, and its magnolol mass fraction is all identical with the little spur type bark of official magnolia with honokiol mass fraction ratio.
In like manner, also can adopt the bark of official magnolia seedling of other type to make stock, bark of official magnolia vegetative shoot is done scion, generally makes stock with the low bark of official magnolia seedling of medicinal material position active constituent content, and the bark of official magnolia vegetative shoot that active constituent content is high is done scion; The bark of official magnolia new varieties that obtain after the grafting, its active constituent content is greatly improved.
Embodiment 2
1) stock is cultivated: select the thunder godvine of Cangnan, Zhejiang to make stock, rugosity is advisable with 1.0-2.5cm, age of tree 1-2,2-4 begins to cultivate before grafting, nursery lot is selected loosing soil, is rich in the sandy soil of organic matter, middle fertility, applied 30-50 kilogram fertilizer in 10-20 days before cultivating and make base fertilizer, plowed soils is to stock stalwartness, standby when neat;
2) scion: select the thunder godvine intermediate form vegetative shoot of quality, Suichang, Zhejiang that kind is good to do scion;
3) grafting: operating personnel's finger, blade, bamboo let are smeared sterilization with the 70-80% medicinal alcohol, sterilization in 1.5-2.5 hour once at interval, the stock cultivated is cut off at place at 9-12cm overhead, and stock is removed growing point and lobus cardiacus, the breach of growing at two cotyledon intermediate vertical down-cutting 1.0-2.0cm; About 2-4cm place is whittled into it in young stem both sides the two-sided wedge shape of 0.5-0.9cm length under the scion cotyledon with blade; The scion folding wedge is inserted gently in the face of accurate stock interface, scion and stock cambium are aimed at, otch is fitted tightly, fix with the sterilization Grafting clip again after 5-8 hour with 150-250 times of formalin bubble, earlier stock and scion are tightened with plastic film at last, then sealing is tightened in scion and stock interface, promptly finish grafting work.
Thunder godvine with the Cangnan, Zhejiang in the foregoing description 2 makes stock, and the thunder godvine intermediate form vegetative shoot of Suichang, Zhejiang is done scion, is to optimize by following result of the test, sees Table 1; And the material mass and the active ingredient of thunder godvine new varieties after the grafting measured, measurement result sees Table 2.
The root of testing used tripterygium wilfordii belongs to thunder godvine and intermediate form thereof, pick up from Suichang, Zhejiang, Cangnan, Wuyi, rivers and mountains, cloud and, the Leqing, become civilized, the individual sample (seeing Table 1) of province cities and counties such as Jinyun, Qingyuan, Lishui, acquisition time is the 10-12 month of 2002 and 2003.Measure because the root skin phloem and the contained composition kind and the content difference of xylem of tripterygium wilfordii root, this test all are divided into each individual sample two positions, get the root of thickness basically identical and study.
As shown in Table 2: the plain average of thunder godvine (stock) first of Cangnan, the preceding Zhejiang of grafting: xylem is 15.7, and phloem is 49.1; The plain average of thunder godvine intermediate form (scion) first of Suichang, the preceding Zhejiang of grafting: xylem is 16.4, and phloem is 64.1; The thunder godvine of Cangnan, Zhejiang effectively become the thunder godvine intermediate form that content is starkly lower than the Suichang, Zhejiang.The plain average of thunder godvine new varieties first after the grafting: xylem is 16.4, and phloem is 64.1, and its active constituent content is identical with the thunder godvine intermediate form of Suichang, Zhejiang.
In like manner, also can adopt the thunder godvine seedling of other type to make stock, the thunder godvine vegetative shoot is done scion, generally makes stock with the low thunder godvine seedling of medicinal material position active constituent content, and the thunder godvine vegetative shoot that active constituent content is high is done scion; The thunder godvine new varieties that obtain after the grafting, its active constituent content is greatly improved.
By the foregoing description 1-2 as can be seen, the medicinal material position content of effective of the bark of official magnolia, thunder godvine new varieties is significantly improved after the grafting.If selecting has other kind seedling of subterranean stem, stem tuber, root system crop to make stock, select crop quality of the same race, vegetative shoot that kind is good to carry out grafting do scion, can improve the quality and the output of its root greatly, reduce cost.Grafting part can be determined according to purpose product harvesting method, goes up masson pine (stock) as wet-land pine tree (scion) grafting and improves rosin output and quality, and grafting part can cut more than the fat mouth; The root and rhizome class is crops, the medicinal material of target product, and grafting part can adopt conventional grafting method to carry out grafting near the ground.
Claims (4)
1. a scion is characterized in that may further comprise the steps to the grafting improved method of stock:
1) stock is cultivated: selecting has the seedling of subterranean stem, stem tuber, root system crop to make stock, rugosity is advisable with 1.0-2.5cm, age of tree 1-2,2-4 begins to cultivate before grafting, nursery lot is selected loosing soil, is rich in the sandy soil of organic matter, middle fertility, applied 30-50 kilogram fertilizer in 10-20 days before cultivating and make base fertilizer, plowed soils is to stock stalwartness, standby when neat;
2) scion: select crop quality of the same race, vegetative shoot that kind is good to do scion;
3) grafting: operating personnel's finger, blade, bamboo let are smeared sterilization with the 70-80% medicinal alcohol, sterilization in 1.5-2.5 hour once at interval, the stock cultivated is cut off at place at 9-12cm overhead, and stock is removed growing point and lobus cardiacus, the breach of growing at two cotyledon intermediate vertical down-cutting 1.0-2.0cm; About 2-4cm place is whittled into it in young stem both sides the two-sided wedge shape of 0.5-0.9cm length under the scion cotyledon with blade; The scion folding wedge is inserted gently in the face of accurate stock interface, scion and stock cambium are aimed at, otch is fitted tightly, fix with the sterilization Grafting clip again after 5-8 hour with 150-250 times of formalin bubble, earlier stock and scion are tightened with plastic film at last, then sealing is tightened in scion and stock interface, promptly finish grafting work.
2. a kind of scion as claimed in claim 1 is characterized in that to the grafting improved method of stock described stock is healthy and strong thunder godvine seedling or bark of official magnolia seedling; Described scion is good thunder godvine vegetative shoot of quality, kind or bark of official magnolia vegetative shoot.
3. a kind of scion as claimed in claim 2 is characterized in that selecting the seedling of the osculant bark of official magnolia to make stock to the grafting improved method of stock, selects little spur type bark of official magnolia quality, vegetative shoot that kind is good to do scion.
4. a kind of scion as claimed in claim 2 is characterized in that selecting the thunder godvine of Cangnan, Zhejiang to make stock to the grafting improved method of stock, and the thunder godvine intermediate form vegetative shoot of Suichang, Zhejiang is done scion.
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070024A (en) * | 2012-03-29 | 2013-05-01 | 中国农业科学院作物科学研究所 | Method for identifying contribution capacity of ground plant nutritive organs to root system |
| CN103342602A (en) * | 2013-07-10 | 2013-10-09 | 句容市乡土树种研究所 | Scion root liquid of hackberry and hackberry grafting and seeding method |
| CN103444446A (en) * | 2013-09-12 | 2013-12-18 | 湖北中烟工业有限责任公司 | Method for improving potassium content of tobaccos by grafting flue-cured tobacco by utilizing grainamaranth |
| CN107155651A (en) * | 2017-06-18 | 2017-09-15 | 余颖恒 | A kind of root system engrafting method of podocarpus |
| CN107155652A (en) * | 2017-06-18 | 2017-09-15 | 余颖恒 | A kind of root system propagation method of silverleaf willow |
| CN107173077A (en) * | 2017-06-18 | 2017-09-19 | 余颖恒 | A kind of root system propagation method of podocarpus |
| CN107173075A (en) * | 2017-06-18 | 2017-09-19 | 余颖恒 | A kind of root system engrafting method of red bayberry |
| CN107182558A (en) * | 2017-06-18 | 2017-09-22 | 余颖恒 | A kind of root system propagation method of araucaria |
| CN107223458A (en) * | 2017-06-18 | 2017-10-03 | 余颖恒 | A kind of root system engrafting method of Chinese yew |
| CN107242076A (en) * | 2017-06-18 | 2017-10-13 | 余颖恒 | A kind of root system propagation method of Chinese juniper |
| CN107258272A (en) * | 2017-06-18 | 2017-10-20 | 余颖恒 | A kind of root system propagation method of horse-tail |
| CN107258334A (en) * | 2017-06-18 | 2017-10-20 | 余颖恒 | A kind of root system engrafting method of metasequoia |
| CN107295901A (en) * | 2017-06-18 | 2017-10-27 | 余颖恒 | A kind of root system engrafting method of horse-tail |
| CN108093910A (en) * | 2017-12-15 | 2018-06-01 | 成都市农林科学院 | A kind of soybean own-rooted tree grafts the method for reducing offspring's cadmium pollution |
| CN108432568A (en) * | 2018-02-10 | 2018-08-24 | 宜州市怀远镇诚信辣椒种植专业合作社 | A kind of engrafting method of pimento |
| CN110447403A (en) * | 2019-08-13 | 2019-11-15 | 中国林业科学研究院亚热带林业研究所 | A kind of Cortex Magnoliae Officinalis propagation by grafiting method |
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| CN103070024A (en) * | 2012-03-29 | 2013-05-01 | 中国农业科学院作物科学研究所 | Method for identifying contribution capacity of ground plant nutritive organs to root system |
| CN103070024B (en) * | 2012-03-29 | 2016-08-03 | 中国农业科学院作物科学研究所 | A kind of plant identification nutrition organs on the ground method to root system contribution ability |
| CN103342602A (en) * | 2013-07-10 | 2013-10-09 | 句容市乡土树种研究所 | Scion root liquid of hackberry and hackberry grafting and seeding method |
| CN103342602B (en) * | 2013-07-10 | 2015-07-08 | 句容市乡土树种研究所 | Scion root liquid of hackberry and hackberry grafting and seeding method |
| CN103444446A (en) * | 2013-09-12 | 2013-12-18 | 湖北中烟工业有限责任公司 | Method for improving potassium content of tobaccos by grafting flue-cured tobacco by utilizing grainamaranth |
| CN107173075A (en) * | 2017-06-18 | 2017-09-19 | 余颖恒 | A kind of root system engrafting method of red bayberry |
| CN107258272A (en) * | 2017-06-18 | 2017-10-20 | 余颖恒 | A kind of root system propagation method of horse-tail |
| CN107173077A (en) * | 2017-06-18 | 2017-09-19 | 余颖恒 | A kind of root system propagation method of podocarpus |
| CN107155651A (en) * | 2017-06-18 | 2017-09-15 | 余颖恒 | A kind of root system engrafting method of podocarpus |
| CN107182558A (en) * | 2017-06-18 | 2017-09-22 | 余颖恒 | A kind of root system propagation method of araucaria |
| CN107223458A (en) * | 2017-06-18 | 2017-10-03 | 余颖恒 | A kind of root system engrafting method of Chinese yew |
| CN107242076A (en) * | 2017-06-18 | 2017-10-13 | 余颖恒 | A kind of root system propagation method of Chinese juniper |
| CN107155652A (en) * | 2017-06-18 | 2017-09-15 | 余颖恒 | A kind of root system propagation method of silverleaf willow |
| CN107258334A (en) * | 2017-06-18 | 2017-10-20 | 余颖恒 | A kind of root system engrafting method of metasequoia |
| CN107295901A (en) * | 2017-06-18 | 2017-10-27 | 余颖恒 | A kind of root system engrafting method of horse-tail |
| CN108093910A (en) * | 2017-12-15 | 2018-06-01 | 成都市农林科学院 | A kind of soybean own-rooted tree grafts the method for reducing offspring's cadmium pollution |
| CN108432568A (en) * | 2018-02-10 | 2018-08-24 | 宜州市怀远镇诚信辣椒种植专业合作社 | A kind of engrafting method of pimento |
| CN110447403A (en) * | 2019-08-13 | 2019-11-15 | 中国林业科学研究院亚热带林业研究所 | A kind of Cortex Magnoliae Officinalis propagation by grafiting method |
| CN110447403B (en) * | 2019-08-13 | 2022-04-22 | 中国林业科学研究院亚热带林业研究所 | Magnolia officinalis grafting propagation method |
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Application publication date: 20110713 |