CN102119626A - Artificial cuttage breeding method of wild Rosa odorata var. gigantea - Google Patents
Artificial cuttage breeding method of wild Rosa odorata var. gigantea Download PDFInfo
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- CN102119626A CN102119626A CN 201010614176 CN201010614176A CN102119626A CN 102119626 A CN102119626 A CN 102119626A CN 201010614176 CN201010614176 CN 201010614176 CN 201010614176 A CN201010614176 A CN 201010614176A CN 102119626 A CN102119626 A CN 102119626A
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Abstract
The invention relates to an artificial cuttage breeding method of wild Rosa odorata var. gigantea. The method comprises the following steps of: selecting a wild Rosa odorata var. gigantea tree which grows for 3-5 years and has favorable development of a tiller branch of the root and stem part as a female parent material; cutting the lignified or semi-lignified raw branch in the current year, which is the stem and root part and is not budded, as a fringe; selecting the tip part and middle section part of the fringe for manufacturing inserted fringe; carrying out right-angle cutting on an upper notch in the cutting position, wherein a lower notch is an inserted fringe base notch; carrying out slope cutting and cleaning disinfection on the node and soaking the node in a root growing powder accelerating agent aqueous solution, and then inserting into a seedling bed according to the plant row space of 30cm*10cm; and after growing and budding the inserted fringe, withdrawing a sunshade screen and entering a natural growth phase. The method disclosed by the invention is simple and convenient and has low cost; on one hand, the method provides sufficient raw material guarantee for the production of a pharmaceutical enterprise and realizes the scientific utilization of plant resources; on the other hand, the method protects wild resources and ecological environment and has favorable market application prospect.
Description
Technical field
The invention belongs to field of plant growing technology, relate to plant asexual multiplication technology, specifically, relate to the artificial cuttage breeding method of the solid public fruit of a kind of medicinal plant.
Background technology
Gu public fruit has another name called big fragrance of a flower moon in water season, the Latin formal name used at school is Rosa odorata (Andr.) Sweet var. gigantea (Cr é p.) Rehd.et Wils., belongs to rose family evergreen shrubs, and fecund is in each province, China southwest.Branch is sturdy, and the leaf alternate is winglike compound leaf, leaflet ovalize, avette or oval shape are avette, be about 2 ~ 7 centimetres, the edge is glued with sharp sawtooth, stipule adhesion or be born on the petiole, Hua Dan generates umbrella room shape, rare multiple umbrella room shape or coniform, fragrance, white, faint yellow or band pink colour, 8 ~ 10 centimetres of diameters, cynarrhodion is oblate spheroid, and seed is sagging.Be used as medicine with root Gu public fruit is many, its flavor is acid flat, and the effect of tool relieving diarrhea with astringents, sterilization is usually used in suffering from diarrhoea, the treatment of bacillary dysentery, and medical value is high, with its Chinese patent drug of making as primary raw material intestines relax sheet and intestines relieving capsule is arranged on the market.Simultaneously because Radix Rosae Giganteae system is flourishing, under ground portion distribute far-reaching, above ground portion sprout tillers ability is stronger, is the important species of deserted mountain, the solid soil of wasteland sand prevention.
Along with the relax continuous increase of sheet and intestines relieving capsule market demand of intestines, the quantity of Radix Rosae Giganteae is also being strengthened, long-term excavating caused the minimizing day by day of wild solid public fruit distribution area, wild resource to suffer serious broken the ring with ecotope.In order to satisfy the protection to the raw material supplying and the natural environment of Radix Rosae Giganteae, the artificial fecundation method of greatly developing solid public fruit becomes problem demanding prompt solution, but does not see the report that success is arranged as yet in the prior art.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, the artificial cuttage breeding method of a kind of survival rate height, with low cost, simple to operate, the wild solid public fruit of being convenient to obtain a large amount of nursery stocks is provided.
Purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, percentage of the present invention is mass percent.
The artificial cuttage breeding method of a kind of wild solid public fruit may further comprise the steps:
(1) the fringe bar is gathered: annual early spring or season in late autumn, select life in 3～5 years, the well-developed wild solid public fruit tree of rhizome position tiller as maternal material, the current-year branch of the clip stem root lignification position, that do not sprout or semi-lignified is as the fringe bar;
It is that 10～20 ℃, humidity are to preserve under 50～80% the calm lucifuge condition that the fringe bar of gathering is positioned over temperature;
The described holding time is 3 ~ 5 days;
(2) plugged ear is made: select fringe bar portion and position, stage casing making plugged ear slightly, off-position is last 1 centimeters of upper cut joint, and the right angle is sheared, and stays 3～4 leaf buds; Lower cut is a plugged ear base portion otch, 0.5～1 centimetre down at joint, and angle is sheared; 3～5 internodes of last lower cut spacing;
The length of plugged ear, hard branch is 10 ~ 15 centimetres, green branch is 15 ~ 20 centimetres.
(3) plugged ear is handled: the base portion otch of plugged ear is immersed in disinfection in the liquor potassic permanganate of 0.2～0.5 % down, liquid level position is 3 ~ 5 centimeters more than the base portion otch; Soak to take out after 10～15 minutes and dry; Take root with the immersion of root-inducing powder promoter again, dry standby;
Described root-inducing powder promoter is a kind of in methyl, indolebutyric acid or the ABT root-inducing powder.
Concentration according to root-inducing powder promoter is determined soak time, when concentration is 50～150mg/L, and soak time 3～10 hours; When concentration is 1500～2500mg/L, soaked 5～15 seconds.
The plugged ear holding time after soaking with root-inducing powder promoter is no more than 12 hours.
(4) prepare in the seedbed: finish the seedbed in 10 days in the past in cuttage and prepared, removed the impurity in the soil, stayed the drain ditch, and sidewalk and hydrophobic just ditch, the seedbed is higher than the drain ditch more than 20 centimetres; Table soil is evenly smooth, and the grogs maximum must not be greater than 3 centimetres; Adjusting nursery soil pH value with ferrous sulfate is 6.5 ~ 7.5, preceding 1～3 day of cuttage, again with the carbendazim of 5 ‰ concentration or formalin solution to the seedbed carry out disinfection, desinsection;
(5) cuttage: will press 10 centimetres of cuttages of seeding row spacing 30 cm x in the seedbed, 3 ~ 5 centimetres of the cuttage degree of depth through the plugged ear after step (3) is handled;
(6) manage after the cuttage: water sufficient water after the cuttage, add a cover shade rate and be 50～60% sunshade net, 30～35 centimetres on sunshade net apart from the surface, seedbed; Water 1～2 time every day later on, keep nursery soil moistening, treat that plugged ear survives the recession of sprouting from the sunshade net, enters the self-sow stage.
With respect to prior art, the present invention has following beneficial effect:
1, by wild solid public fruit is carried out artificial cottage propagation; not only the production for medicine company enterprise provides sufficient raw material to guarantee; realized the scientific utilization of plant resources, and protected wild resource, for ecological environmental protection, prevent that the large tracts of land water and soil loss from providing effective guarantee.
2, survival rate height after easy, with low cost, the cuttage of the inventive method has better market prospect.
Embodiment
By specific embodiment given below; can the present invention is described in further detail; but be to be understood that; embodiment only is an explanation of the invention; and limit the invention never in any form; various changes or modification that those skilled in the art do according to spirit of the present invention fall within protection scope of the present invention equally.
Embodiment 1
In Simao, Yunnan and wild country, area, Mengzi, early spring or season in late autumn then, select life in 3～5 years, the well-developed wild solid public fruit tree of rhizome position tiller as maternal material, the current-year branch of the clip stem root lignification position, that do not sprout or semi-lignified is as the fringe bar;
It is that 10～20 ℃, humidity are to save as 3 ~ 5 days under 50～80% the calm lucifuge condition that the fringe bar of gathering is positioned over temperature; In case of necessity, artificial dispersing dry defecates clean water and gives cooling moisture-retaining, also can soak fringe bar base portion and preserve;
Select fringe bar portion and position, stage casing making plugged ear slightly, off-position, last 1 centimeters of upper cut joint, the right angle is sheared, and stays 3～4 leaf buds; Lower cut is a plugged ear base portion otch, 0.5～1 centimetre down at joint, and angle is sheared; 3～5 internodes of last lower cut spacing, the hard branch of the length of plugged ear is 10 ~ 15 centimetres, classifies, ties up after plugged ear is carried out, base portion is placed downwards;
The base portion otch of plugged ear is immersed in the 0.5 % liquor potassic permanganate down plugged ear is carried out sterilization processing, soak time is taken out after 10 minutes and is dried; Working concentration is that the root-inducing powder promoter naphthalene acid solution of 50 ~ 150mg/L soaks the plugged ear base portion, soaks 3 hours, dries standbyly, is no more than 12 hours with the plugged ear holding time after the immersion of root-inducing powder promoter.
Finish the seedbed in 10 days in the past in cuttage and prepared, removed the impurity in the soil, stayed the drain ditch, sidewalk and hydrophobic just ditch, the seedbed is higher than the drain ditch more than 20 centimetres; Table soil is evenly smooth, and the grogs maximum must not be greater than 3 centimetres, no weeds, foreign material, and slotting machine basal plane basic horizontal, it is ventilative to be beneficial to draining; Adjusting nursery soil pH value with ferrous sulfate is 6.5, preceding 1～3 day of cuttage, again with the carbendazim solution of 5 ‰ concentration to the seedbed carry out disinfection, desinsection;
Plugged ear is after treatment pressed 10 centimetres of cuttages of seeding row spacing 30 cm x in the seedbed, 3 ~ 5 centimetres of the cuttage degree of depth;
Water sufficient water after the cuttage, add a cover the concealment rate and be 50～60% sunshade net, 30～35 centimetres on sunshade net apart from the surface, seedbed; Water 1～2 time every day later on, keep nursery soil moistening, treat that plugged ear survives the recession of sprouting from the sunshade net, enters the self-sow stage.
Embodiment 2
Repeat embodiment 1, following difference is arranged: gather maternal material season in late autumn in then.
Embodiment 3
Repeat embodiment 1, following difference arranged: preceding 1～3 day of cuttage, again with the formalin solution of 5 ‰ concentration to the seedbed carry out disinfection, desinsection.
Embodiment 4
Repeat embodiment 1, following difference is arranged: when selecting green branch as plugged ear, its length is 15 ~ 20 centimetres.
Embodiment 5
Repeat embodiment 1, following difference is arranged: the base portion otch of plugged ear is immersed in the 0.2 % liquor potassic permanganate down plugged ear is carried out sterilization processing, soak time is taken out after 15 minutes and is dried.
Embodiment 6
Repeat embodiment 1, following difference is arranged: use the root-inducing powder promoter naphthalene acid solution of 50 ~ 150mg/L to soak the plugged ear base portion, soaked 6 hours.
Embodiment 7
Repeat embodiment 1, following difference is arranged: working concentration is that the root-inducing powder promoter naphthalene acid solution of 50 ~ 150mg/L soaks the plugged ear base portion, soaks 10 hours.
Embodiment 8
Repeat embodiment 1, following difference is arranged: working concentration is that the root-inducing powder promoter naphthalene acid solution of 1500 ~ 2500mg/L soaks the plugged ear base portion, soaks 5 seconds.
Embodiment 9
Repeat embodiment 1, following difference is arranged: working concentration is that the root-inducing powder promoter naphthalene acid solution of 1500 ~ 2500mg/ soaks the plugged ear base portion, soaks 10 seconds.
Embodiment 10
Repeat embodiment 1, following difference is arranged: working concentration is that the root-inducing powder promoter naphthalene acid solution of 1500 ~ 2500mg/ soaks the plugged ear base portion, soaks 15 seconds.
Embodiment 11
Repeat embodiment 1, following difference is arranged: root-inducing powder promoter is adopted heteroauxin.
Embodiment 12
Repeat embodiment 1, following difference is arranged: root-inducing powder promoter is adopted the ABT root-inducing powder.
Embodiment 13
Repeat embodiment 1, following difference is arranged: use ferrous sulfate to adjust nursery soil pH value 7.0.
Embodiment 14
Repeat embodiment 1, following difference is arranged: use ferrous sulfate to adjust nursery soil pH value 7.5.
Claims (7)
1. the artificial cuttage breeding method of a wild solid public fruit may further comprise the steps:
(1) the fringe bar is gathered: annual early spring or season in late autumn, select life in 3～5 years, the well-developed wild solid public fruit tree of rhizome position tiller as maternal material, the current-year branch of the clip stem root lignification position, that do not sprout or semi-lignified is as the fringe bar;
(2) plugged ear is made: select fringe bar portion and position, stage casing making plugged ear slightly, off-position is last 1 centimeters of upper cut joint, and the right angle is sheared, and stays 3～4 leaf buds; Lower cut is a plugged ear base portion otch, 0.5～1 centimetre down at joint, and angle is sheared; 3～5 internodes of last lower cut spacing;
(3) plugged ear is handled: the base portion otch of plugged ear is immersed in disinfection in the liquor potassic permanganate of 0.2～0.5 % down, liquid level position is 3 ~ 5 centimeters more than the base portion otch; Soak to take out after 10～15 minutes and dry; Take root with the immersion of root-inducing powder promoter again, dry standby;
(4) prepare in the seedbed: finish the seedbed in 10 days in the past in cuttage and prepared, removed the impurity in the soil, stayed the drain ditch, and sidewalk and hydrophobic just ditch, the seedbed is higher than the drain ditch more than 20 centimetres; Table soil is evenly smooth, and the grogs maximum must not be greater than 3 centimetres; Adjusting nursery soil pH value with ferrous sulfate is 6.5 ~ 7.5, preceding 1～3 day of cuttage, again with the carbendazim of 5 ‰ concentration or formalin solution to the seedbed carry out disinfection, desinsection;
(5) cuttage: the plugged ear after step (3) is handled is pressed 10 centimetres of cuttages of seeding row spacing 30 cm x in the seedbed, 3 ~ 5 centimetres of the cuttage degree of depth;
(6) manage after the cuttage: water sufficient water after the cuttage, add a cover shade rate and be 50～60% sunshade net, 30～35 centimetres on sunshade net apart from the surface, seedbed; Water 1～2 time every day later on, keep nursery soil moistening, treat that plugged ear survives the recession of sprouting from the sunshade net, enters the self-sow stage.
2. the artificial cuttage breeding method of wild solid public fruit according to claim 1 is characterized in that, it is that 10～20 ℃, humidity are to preserve under 50～80% the calm lucifuge condition that the fringe bar of collection is positioned over temperature.
3. the artificial cuttage breeding method of wild solid public fruit according to claim 2 is characterized in that the described fringe bar holding time is 3 ~ 5 days.
4. the artificial cuttage breeding method of wild solid public fruit according to claim 1 is characterized in that, the length of plugged ear is in the described step (2), 10 ~ 15 centimetres in hard branch, 15 ~ 20 centimetres in green branch.
5. the artificial cuttage breeding method of wild solid public fruit according to claim 1 is characterized in that, the root-inducing powder promoter in the described step (3) is a kind of in methyl, indolebutyric acid or the ABT root-inducing powder.
6. the artificial cuttage breeding method of wild solid public fruit according to claim 1 is characterized in that step is determined soak time according to the concentration of root-inducing powder promoter in (3), promptly when concentration is 50～150mg/L, and soak time 3～10 hours; When concentration is 1500～2500mg/L, soaked 5～15 seconds.
7. the artificial cuttage breeding method of wild solid public fruit according to claim 1 is characterized in that, is no more than 12 hours with the plugged ear holding time after the immersion of root-inducing powder promoter in the step (3).
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668900A (en) * | 2012-05-22 | 2012-09-19 | 句容美华园林景观发展有限公司 | Tilia miqueliana rapid breeding method |
| CN104322261A (en) * | 2014-11-13 | 2015-02-04 | 张家港耕耘家庭农场有限公司 | Efficient rosa chinensis propagation method |
| CN104350931A (en) * | 2014-11-25 | 2015-02-18 | 中国科学院成都生物研究所 | Cutting propagation method for wild hybrid rose |
| CN105265152A (en) * | 2015-10-31 | 2016-01-27 | 阜南县黄岗镇工艺柳编厂 | Salix integra cutting and seedling method |
| CN105284347A (en) * | 2015-10-14 | 2016-02-03 | 中国科学院昆明植物研究所 | Technology for propagating psammosilene tunicoides by fast-growing cuttage |
| CN106489491A (en) * | 2016-10-24 | 2017-03-15 | 怀化学院 | A kind of store method of Pasania cuspidata cutting vigor and application |
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| CN101416586A (en) * | 2007-10-26 | 2009-04-29 | 李巧英 | The cottage method of a kind of Chinese rose |
| CN101627696A (en) * | 2009-08-24 | 2010-01-20 | 李姗 | Method for improving cuttage survival rate of rose |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416586A (en) * | 2007-10-26 | 2009-04-29 | 李巧英 | The cottage method of a kind of Chinese rose |
| CN101627696A (en) * | 2009-08-24 | 2010-01-20 | 李姗 | Method for improving cuttage survival rate of rose |
Non-Patent Citations (1)
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| 《河北林业科技》 20080430 董法金 腾本月季栽培技艺及应用 第59-60页 1-7 , 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668900A (en) * | 2012-05-22 | 2012-09-19 | 句容美华园林景观发展有限公司 | Tilia miqueliana rapid breeding method |
| CN104322261A (en) * | 2014-11-13 | 2015-02-04 | 张家港耕耘家庭农场有限公司 | Efficient rosa chinensis propagation method |
| CN104350931A (en) * | 2014-11-25 | 2015-02-18 | 中国科学院成都生物研究所 | Cutting propagation method for wild hybrid rose |
| CN105284347A (en) * | 2015-10-14 | 2016-02-03 | 中国科学院昆明植物研究所 | Technology for propagating psammosilene tunicoides by fast-growing cuttage |
| CN105284347B (en) * | 2015-10-14 | 2019-11-26 | 中国科学院昆明植物研究所 | Tuniclike psammosilene root fast-growing Technique on Cuttage Propagation |
| CN105265152A (en) * | 2015-10-31 | 2016-01-27 | 阜南县黄岗镇工艺柳编厂 | Salix integra cutting and seedling method |
| CN106489491A (en) * | 2016-10-24 | 2017-03-15 | 怀化学院 | A kind of store method of Pasania cuspidata cutting vigor and application |
| CN106489491B (en) * | 2016-10-24 | 2020-06-02 | 怀化学院 | Preservation method and application of lithocarpus polystachyus rehd cutting vitality |
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| CN102119626B (en) | 2012-05-23 |
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Owner name: YUNNAN CONBA PLANT EXTRACTS CO., LTD. Free format text: FORMER OWNER: XIONGYE PHARMACEUTICAL INDUSTRY CO., LTD., YUNNAN Effective date: 20121113 |
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Effective date of registration: 20160204 Address after: 650217 Yunnan city of Kunming Province Economic and Technological Development Zone No. 1 and Taolu Patentee after: Yunan Xitao Green Pharmaceutical Co., Ltd. Address before: 655000 West Road, Qujing economic and Technological Development Zone, Yunnan Patentee before: Yunnan Conba Plant Extracts Co., Ltd. |
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Address after: 655000 West Road, Qujing economic and Technological Development Zone, Yunnan Patentee after: Yunnan kangenbeixitao Pharmaceutical Co., Ltd Address before: 650217 Yunnan city of Kunming Province Economic and Technological Development Zone No. 1 and Taolu Patentee before: YUNNAN XITAO GREEN PHARMACEUTICAL Co.,Ltd. |
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