CN102106296B - Method for cultivating novel Dingyuan pig breed - Google Patents
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Abstract
The invention provides a method for cultivating a novel Dingyuan pig breed, which relates to a method for cultivating a novel pig breed. The invention provides the method for cultivating the novel Dingyuan pig breed which has the advantages high fecundity, good meat quality and strong stress resistance of Dingyuan pigs as well as the characteristics of fast growth, high lean meat percentage and the like of imported pigs. The method comprises the following steps: a Dingyuan sow is hybridized with a novel USA strain Duroc boar to cultivate a first generation sow core group; second grading hybridization is performed to the first generation sow core group and a Dingyuan boar or the novel USA strain Duroc boar; from the third generation, group closure propagation and breeding are adopted respectively, and crossing of two to three generations is performed; and selection and remaining are performed through methods of comprehensive selection indexes and molecule marking assistance selection to form the novel Dingyuan pig breed, namely a novel breed of Wan Huai pigs and a novel breed of Dingyuan-Duroc pigs.
Description
Technical field
The present invention relates to the breeding method of a boar new lines, particularly relate to a kind of breeding method for Dingyuan pig new lines.
Background technology
China is that Swine Production and pork are consumed the first big country in the world, and almost half swine rearing is in China in the world.Pork is China tradition meat product source, accounts for 66% of meat product consumption.Because the breeding technique problem, the premium muscle type boar of China market demand but depends on import for a long time, and stock breeding is in the poor circulation of " introducing a fine variety → keep → degenerate → introduce a fine variety again "; The growth of comparing China's local pig breed is slow, lean meat percentage is low, and the high lean meat percentage of duroc, Large White and Landrace and supporting hybrid combination thereof, high growth rate and feed efficiency have become the dominant pattern that the Chinese commodity pig produces.Pig kind meat is poor, fertility is hanged down and made the efficient of its utilization and sustainability be subject to great challenge but these are introduced.For this reason, the scientific research of pig kind exporting country also towards improving meat quality, adapts to high quality pork and the organic meat future development of consumer's taste.Utilize the performance that Taihu Lake pig fertility is high, meat is good to cultivate the maternal side " Kang Beier (Campbell) " that makes new advances such as PIC company, and with 402 paternal hybridization, forming fixing 5 is the assorted excellent pig of forming a complete production network, thereby it is low to have solved to some extent fertility, the contradiction that meat is poor.The in recent years specialized strains such as PIC, DeKalb, Sehgers and Topigs introduction and the popularization of assorted excellent pig that form a complete production network, new breeding substitutes and upgrading so that China's Swine Production is faced with, and the breeding work of China's pig specialized strain has been put on schedule.
In the past few decades, China the seed selection of local pig breed improve and and the crossbreeding and improvement of external pig kind and heterosis utilization aspect made a large amount of work, greatly improved the level of production of China's pig industry.Along with the development of molecular biology and molecular quantitative, the breeding of pig is progressively moved towards the road that conventional breeding combines with molecular breeding from conventional breeding.Oneself is applied to the combination of the allelomorph of the different lines of pig or kind, the marker assisted selection of proterties molecular labeling, has greatly improved accuracy and the breeding efficiency selected.
Hair color receives much concern in pig variety (strain) is cultivated, differentiate and fixedly hair color be the important step of pig breeding.Mammal many and melanocytic growth, gene that variation is relevant with regulation and control have been identified by the research of hair color variations, wherein MC1R plays central role in the melanocytic eumelanin of mammal (black/brown) and phaeomelanin (redness/yellow) are synthetic, and by the coded by said gene in quilt hair shade (E) site, the wild type of many animals relates to these two kinds melanic synthesizing.In a kind of mammal, the dominant allele of exit site can produce a kind of black of even one, and (Searle 1968 by hair; And the Recessive alleles in this site can enlarge the ratio of red/uranidin Jackson1997).The sudden change of MC1R gene can change pigment synthetic of some animals (ox, horse, fox, chicken, mouse, pig, people etc.), the present Study on Molecular Mechanism that produces of existing black wool pig, palm fiber pig and piebald pig, but not yet be applied to rearing new variety.Chinese scholar conducts in-depth research and inquires into the molecular genetic marker of bristles color, wherein the research group that leads of applicant has found the mutational site that the MC1R gene is new, confirm that Dingyuan pig and duroc hair color difference can be distinguished by the variation of a restriction enzyme site, simple, the easy row of detection method can directly apply to the discriminating of black wool pig homozygote and heterozygote.
Fat is one of main component of body, is to keep the necessary material base of normal activities, and also being affects quality of pig trunk and consumer to the key factor of products taste, local flavor and succulence demand.Chinese native pig breed and the meat difference of introducing between the pig kind often are used as the material of cultivating dissimilar products.There is significant difference in the meat performance of Chinese native pig breed and introducing pig kind, wherein the Chinese native pig breed content of fat in body exceeds about 15% than introduced variety, be referred to as lard type and bacon hogs by people, embody the difference of a series of Fatty synthesis metabolism of body.For verifying its specific genetic background and fat metabolism mechanism, the steroids gene that the researcher has successively resolved the key gene in the fat metabolism process and played the physiological regulating control effect, and many molecular labelings related with body fat content have therefrom been obtained, for the pig kind is cultivated and carcass trait is improved foundation is provided, but this mark really can be used very few for us.On function, hormone regulating and controlling levels level is clearly demarcated.Recently the thyroid hormone of finding is replied Spot14 (Thyroid hormone responsive spot 14, THRSP) be exactly to be subjected to one of gene of thyroid hormone abduction delivering in the nuclear, the albumen of its gene code claims again Spot14 or S14, is the transcription factor that the pth receptor regulation and control activate.THRSP albumen can stimulate thyroxine, the high glucose level is produced responsing reaction, and the chromosomal region at this gene place is relevant with obesity, thereby this gene is considered to, and generation has important regulating and controlling effect to fat.At present, people, rat and mouse are more common in the research of this gene, the in recent years research on the livestock and poultry such as chicken, duck is also more and more.Research to pig THRSP gene, Chen Hua etc. (2010) have cloned and have analyzed the characteristic of THRSP gene 5 ' control region, the applicant utilizes above-mentioned pig kind in the difference of producing on the fat ability, by detecting the genetic variation of THRSP gene coding region, distribution and the group dynamics thereof of research gene between difference product fat ability pig kind, the genetic variation of discovery THRSP gene coding region and pig produce between the fat ability has significant relevance.
The heritability of litter size of pig very low (about 0.1) adopts in the conventional breeding method in family selection or the family and selects relative efficiency.In the molecular breeding technology, the normal utilization candidate gene relevant with the high litter trait of pig carries out marker assisted selection, abroad the result of study of some marker gene has wherein also been applied for patent, such as female hormone receptor gene (ESR) and estrogen receptor beta subunit gene (FSH β) etc.Molecular genetic marker is applied to that marker assisted selection and mark are auxiliary to infiltrate for various reasons, still is in conceptual phase, and characters of number born is subjected to the impact of the factors such as environment larger in addition, the breeding method of litter size is remained to be paid more make great efforts research and development.
The animal varieties seed selection generally all relates to multiple characters and selects, and the multiple characters system of selection generally has tandem method (or claiming the individual event back-and-forth method), independent culling method and comprehensive selection method.Wherein the comprehensive selection method has the highest selection effect, is the system of selection that is most widely used in animal breeding so far.In the comprehensive selection method, because the selection index method tool has an enormous advantage, thereby promptly grow up in theoretical and method, develop into restricted selection index, the most suitable selection index, aggregate breeding value estimation and General Selection Index etc. from general multiple selection index, thereby become the important method of multiple characters selection.Because the not impact of equal many factors of the proterties of selecting difference, utilization difference in period and character weight, the multiple selection index of formulating varies.
Summary of the invention
The invention provides and a kind ofly both can keep that Dingyuan pig fertility is strong, meat good, the advantage of strong stress resistance, absorbed again that the growth of introducing pig variety is fast, the breeding method of the Dingyuan pig new lines of lean meat percentage high.This method is carried out the selection of litter size by multiple selection index; Select hair color by molecular labeling MC1R gene, select meat, successful by molecular labeling THRSP gene.
The technical scheme that realizes the foregoing invention purpose is as follows:
A kind of breeding method of Dingyuan pig new lines, it may further comprise the steps:
Phase I:
With the excellent individual of high-quality, high yield, degeneration-resistant Anhui Native kind Dingyuan pig as female parent, hybridize as male parent with the excellent individual that derives from different familys in the new American Duroc pig boar of external lean meat species kind, the sow of selecting and remain in generation in institute postpartum forms the 1st sow core group from generation to generation;
Second stage:
Take the 1st from generation to generation the sow core group carry out grading up as maternal, Dingyuan pig boar as male parent, Dingyuan pig boar derives from different familys, in giving birth to offspring with molecular labeling and the multiple selection index method sow of selecting and remain, Huaihe River, called after Anhui pig, form the 2nd generation Huaihe River, Anhui pig sow core group, it contains 75% Dingyuan pig blood lineage, 25% duroc blood lineage;
Carry out grading up take the 1st generation sow core group as maternal, new American Duroc pig boar as male parent, Duroc boars derives from different familys, in giving birth to offspring with molecular labeling and the multiple selection index method sow of selecting and remain, called after restrains pig surely, form the 2nd and surely restrain pig sow core group from generation to generation, it contains 75% duroc blood lineage, 25% Dingyuan pig blood lineage;
Phase III:
Take the 2nd generation Huaihe River, Anhui pig sow core group as maternal, carry out grading up take Dingyuan pig boar as male parent again, Dingyuan pig boar derives from different familys, in giving birth to offspring with molecular labeling and multiple selection index method select and remain boar and sow, form the 3rd generation Huaihe River, Anhui pig core group, it contains 87.5% Dingyuan pig blood lineage, 12.5% duroc blood lineage;
Surely restrain from generation to generation pig sow core group as maternal take the 2nd, carry out grading up take new American Duroc pig pig boar as male parent again, Duroc boars derives from different familys, in giving birth to offspring with molecular labeling and multiple selection index method select and remain boar and sow, form the 3rd and surely restrain the pig core group from generation to generation, it contains 87.5% duroc blood lineage, 12.5% Dingyuan pig blood lineage;
The quadravalence section:
Respectively with the male and female pig mating in the 3rd generation Huaihe River, the Anhui pig core group, with the 3rd male and female pig mating that surely restrains from generation to generation in the pig core group, adopt respectively from generation to generation colony's locking breeding and seed selection since the 3rd, carry out the traversed by of 2～3 generations, traversed by each for pig in molecular labeling and multiple selection index method select and remain boar and sow, obtain traversed by respectively for the pig core group.Same generation boar and sow mating, each select and remain from generation to generation sow, boar obtain new lines Anhui Huaihe River pig and new lines and surely restrain pig, it is Dingyuan pig new lines.
The performance indications of Huaihe River, above-mentioned new lines Anhui pig are:
Reproductive performance: Huaihe River, the Anhui average nest litter size of pig core group multiparity sow reaches 12.99, and nest is lived young several 12.50; Be on close level, be higher than duroc with the Dingyuan pig;
Growth rate: it is 180 ages in days that the market pig body weight reaches time of 90 kilograms, and the price of deed is 3.35: 1; Be higher than the Dingyuan pig far away, be lower than duroc;
Trunk and meat quality: lean meat percentage is 52%～56%, and intramuscular fat is 3.3%～4.0%, without yellowish pink pale, quality is soft, tangent plane oozes out and dark, strong, dry, marble grain scoring 3.3～3.5 yellowish pinkly marks 3.2～3.6; Lean meat percentage is higher than the Dingyuan pig, a little less than duroc.
The performance indications that above-mentioned new lines restrains pig surely are:
Reproductive performance: surely restrain the average nest litter size of pig core group multiparity sow and reach 11.58, nest is lived young several 11.36; Be on close level, be higher than duroc with the Dingyuan pig;
Growth rate: it is 145 ages in days that the market pig body weight reaches time of 100 kilograms, and the price of deed is 2.78: 1; Be higher than the Dingyuan pig far away, be on close level with duroc;
Trunk and meat quality: lean meat percentage is 64%～68%, and intramuscular fat is 3.1%～3.5%, without yellowish pink pale, quality is soft, tangent plane oozes out and dark, strong, dry, marble grain scoring 3.0～3.5 yellowish pinkly marks 3.2～3.5; Lean meat percentage is higher than the Dingyuan pig far away, is on close level with duroc.
The breeding method of a kind of Dingyuan pig of the present invention new lines, the selection of wherein said the 1st generation sow core group is:
For the first time select and remain and will carry out when 28 ages in days weans: select by a hair black, body weight is greater than 4kg, more than 7 pairs of numbers of nipples reach, and rounded, the sow that physique is good;
Select and remain for the second time and carry out when 4 monthly age: superseded growth rate is slow, the pig that physique is poor;
Select and remain for the third time and when 6 monthly age, carry out: measure 6 monthly age body weight, the thickness of backfat, the rear daily gain to the 90kg body weight stage of calculating wean, feedstuff-meat ratio, select that daily gain 500g is above, feedstuff-meat ratio less than 3.2: 1, during 90kg below the live body thickness of backfat 20mm, eye muscle area is greater than 25cm
2Sow reserve seed for planting.
The breeding method of a kind of Dingyuan pig of the present invention new lines, wherein said the 2nd generation Huaihe River, Anhui pig sow core group and the 2nd selection that surely restrains pig sow core group from generation to generation are:
Huaihe River, Anhui pig carries out selecting and remain from birth to weaning period the first time, should be according to the testing result of molecular genetic marker, and the MC1R genotype of selecting and remain is AA, namely by hair black, the THRSP genotype is AA and GA; The wean of 28 ages in days the time is carried out selecting and remain second time, requires that the bodily form is good, physique is good, physically well develops, and calculates the wean multiple selection index according to wean individual weight and the young number of brood work again, determines the individuality of reserving seed for planting according to the multiple selection index that weans is descending; Select and remain for the third time during 4 monthly age, depauperation, the pinto pig of quilt hair are eliminated; Again according to wean multiple selection index, 4 monthly age individual weights and the long 4 monthly age multiple selection indexs that calculate of body, determine the individuality of reserving seed for planting according to 4 monthly age multiple selection indexs are descending; When 6 monthly age, carry out the 4th tight choosing, calculate 6 monthly age multiple selection indexs according to the thickness of backfat that 4 monthly age multiple selection indexs, 6 monthly age individual weights and B ultrasonic record, select according to 6 the descending of monthly age multiple selection index;
Surely restrain pig just from birth to weaning period, carry out selecting and remain the first time, should be according to the testing result of molecular genetic marker, it is brown by hair to select and remain, and the THRSP genotype is AA and GA; The wean of 28 ages in days the time is carried out selecting and remain second time, requires that the bodily form is good, physique is good, physically well develops, and calculates the wean multiple selection index according to wean individual weight and the young number of brood work again, determines the individuality of reserving seed for planting according to the multiple selection index that weans is descending; Select and remain for the third time during 4 monthly age, depauperation, the pinto pig of quilt hair are eliminated; Long according to wean multiple selection index, 4 monthly age individual weights and body again, calculate 4 monthly age multiple selection indexs, foundation 4 monthly age multiple selection indexs are descending determines the individuality of reserving seed for planting; When 6 monthly age, carry out the 4th tight choosing, calculate 6 monthly age multiple selection indexs according to the thickness of backfat that 4 monthly age multiple selection indexs, 6 monthly age individual weights and B ultrasonic record, select according to 6 the descending of monthly age multiple selection index;
From through above-mentioned select and remain for four times the 2nd from generation to generation Huaihe River, Anhui pig sow and the 2nd surely restrain from generation to generation and all carry out intensity with 3: 1 ratio the replacement gilt of pig sow and select and remain, be 3 ratios that go out first among the sow group, obtain the 2nd generation Huaihe River, Anhui pig sow core group and the 2nd and surely restrain pig sow core group from generation to generation.
Black wool is dominant for palm fiber, and it is the pig of AA that Huaihe River, Anhui pig is selected the MC1R genotype, and having rejected the MC1R genotype is the heterozygote of AG, and duroc selection MC1R genotype is the pig of GG, is brown pig by hair namely, and the hair color of new lines pig is fixed.
The breeding method of a kind of Dingyuan pig of the present invention new lines, wherein said the 3rd generation Huaihe River, Anhui pig core group, the 3rd surely restrains the pig core group from generation to generation and traversed by respectively for pig core group selection is:
Piglet carries out selecting and remain from birth to weaning period the first time, should be according to the testing result of molecular genetic marker, and the THRSP genotype is AA and GA;
Carry out selecting and remain the second time during wean of piglet 28 ages in days, heavy and the young number calculating of the brood work wean multiple selection index according to weaned piglet, determine the individuality of reserving seed for planting according to the wean multiple selection index is descending, eliminate simultaneously that physique is poor, depauperation and by the pinto pig of hair-videotape;
When 4 monthly age, select and remain for the third time, according to wean multiple selection index, 4 monthly age individual weights and the long 4 monthly age multiple selection indexs that calculate of body, determine the individuality of reserving seed for planting according to 4 monthly age multiple selection indexs are descending, eliminate simultaneously depauperation and by the pinto pig of hair;
6 monthly ages carried out the 4th time and select and remain, and calculated 6 monthly age multiple selection indexs according to 4 monthly age multiple selection indexs, 6 monthly age individual weights and the thickness of backfat, determined the individuality of reserving seed for planting according to 6 monthly age multiple selection index sizes, eliminated simultaneously hypogenetic pig;
From the replacement gilt through above-mentioned male and female pig of selecting and remain for four times, the male and female pig all carries out intensity with 3: 1 ratio selects and remain, and namely is 3 ratios that go out first in the male and female swinery.
The breeding method of a kind of Dingyuan pig of the present invention new lines, wherein:
The described generation interval be the 1st from generation to generation sow core group, the 2nd from generation to generation Huaihe River, Anhui pig sow core group, the 2nd surely restrain from generation to generation pig sow core group, the 3rd from generation to generation Huaihe River, Anhui pig core group, the 3rd surely restrain pig core group and each time interval of successively breeding for pig of traversed by from generation to generation, Huaihe River, Anhui pig is in 5～7 monthly ages, body weight 80～90kg, surely restrain pig and when 6～8 monthly ages, body weight 100kg～120kg, concentrate breeding, should avoid full sibs and half-sib mating during breeding; 2 respectively select and remain through 4 times for pig from generation to generation and later on, concentrate breeding March in next year again; The piglet of primiparity is reserved seed for planting, 1 year generation.
The auxiliary seed choosing method MC1R gene of described molecular labeling is selected hair color, and the THRSP gene is selected meat, adopts the ear tissue sample when piglet is born, and returns the laboratory and carries out Markers for Detection, selects to reserve seed for planting and eliminate according to the genotype that molecular labeling goes out.
The genotypic detection method of MC1R:
Extract determined pig genomic DNA, take the genomic DNA that extracts as template, MRAG-S and MRAG-A are that primer carries out pcr amplification, and the nucleotide sequence of primer MRAG-S and MRAG-A is as follows:
MRAG-S：GCGGGACGATGCCTGTGCTT，
MRAG-A：GGTAGCGCAGCGCGTAGAAGAT
Pcr amplification system: upstream and downstream primer, PCR buffer, dNTPs, Taq archaeal dna polymerase, dna profiling, ddH
2O;
The pcr amplification program: denaturation, annealing, 4 ℃ of preservations are extended in circulation;
With NspI digestion with restriction enzyme PCR product;
Endonuclease reaction system: PCR product, NspI restriction endonuclease, buffer, ddH
2O;
Enzyme is cut, and agarose gel electrophoresis detects, and takes pictures and differentiates genotype with the gel imaging instrument;
NspI enzyme cutting type, the black wool pig is homozygous to be AA, and brown hair is homozygous to be GG, and the heterozygous of black wool pig is GA.See Fig. 1;
The genotypic detection method of THRSP:
Extract determined pig genomic DNA; Take the genomic DNA that extracts as template, TP2-S and TP2-A are that primer carries out pcr amplification, and the nucleotide sequence of primer TP2-S and TP2-A is as follows:
TP2-S：TACCCCAAGAACTGCCTGCTGA
TP2-A：GGTGGAACTGGGCTTCTAGGTC
Pcr amplification system: upstream and downstream primer, PCR buffer, dNTPs, Taq archaeal dna polymerase, dna profiling, ddH
2O;
The pcr amplification program: denaturation, annealing, 4 ℃ of preservations are extended in circulation;
With MvaI and PvuII digestion with restriction enzyme PCR product;
The reaction system that enzyme is cut: PCR product, restriction endonuclease, buffer, ddH
2O;
Enzyme is cut, and agarose gel electrophoresis detects, and takes pictures and differentiates genotype with the gel imaging instrument;
Cut the PCR products of dissimilar pigs with the PvuII enzyme, obtain 3 kinds of genotype, wherein select and remain AA and GA genotype are seen Fig. 2; Cut the PCR products of dissimilar pigs with the MvaI enzyme, obtain 3 kinds of genotype, wherein select and remain AA and G.A genotype are seen Fig. 3;
Described different phase multiple selection index is as follows:
Wean multiple selection index=wean individual weight * 40%/weanling weight average+young the number of brood work * 60%/young number average value of work.
The long average of long * 30%/body of 4 monthly ages multiple selection index=wean multiple selection index * 20%+4 monthly age individual weight * 50%/body weight average+body.
The individual 6 monthly age individual weights of 6 monthly age multiple selection indexs=4 monthly ages multiple selection index * 20%+ * 50%/body weight average+6～7 ribbed back fat thickness * 30%/6～7 ribbed back fat thickness averages.
The breeding method of a kind of Dingyuan pig of the present invention new lines provides a kind of both can keep that Dingyuan pig fertility is strong, meat good, the advantage of strong stress resistance, had absorbed again that the growth of introducing pig variety is fast, the breeding method of the Dingyuan pig new lines of lean meat percentage high.The present invention improves litter size, growth rate and carcass lean meat percentage, successful by multiple selection index; Select hair color by molecular labeling MC1R gene, select meat by molecular labeling THRSP gene, effect is remarkable.Inherited outside the height farrowing of local pig breed according to the fixed gram pig that this method is cultivated, meat, growth rate and lean meat percentage are higher than prior art; Meat, the reproductive performance of Huaihe River, Anhui pig are better than prior art, and growth rate is near prior art.
Description of drawings
Fig. 1 is that the NspI enzyme is cut genotype, and 2,3,4,6,8 and 9 is the GG type, and 1,5 and 7 is AG types, the 10th, AA type
Fig. 2 is THRSP gene PvuII cleavage map: 1 and 2 roads are AA types; 3 and 4 roads are GG types; 5 and 6 roads are GA types.
Fig. 3 is THRSP gene M vaI cleavage map: 7 and 8 roads are AA types; 9 and 10 roads are GG types; 11 and 12 roads are GA types.
Fig. 4 is that the breeding method of a kind of Dingyuan pig of the present invention new lines is cultivated route map.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated by embodiment.
Embodiment:
The Dingyuan pig
Producing region centered by the Dingyuan County, Anhui Province, the meat fat dual-purpose type pig kind that forms through seed selection at Yangze river and Huai river knob Long-term breeding.Mainly be distributed in Yangze river and Huai river knob, Anhui Province.The Dingyuan pig has three kinds of builds, i.e. large pleat skin, two thin skins (cucumber strip), field cricket.Large pleat skin build is larger, and the field cricket build is less, and two thin skin (cucumber strip) builds are medium.Existing its figure and features feature: build is medium longer and narrower mainly take two thin skins (cucumber strip) as main, and physique is healthy and strong, by hair black, the brown fine hair of close life in winter; The flat outspoken length of the light face of head, volume is streamlined with head, and forehead has the rhombus wrinkle, and ear hangs down to greatly the corners of the mouth, and neck is elongated; The straight buttocks of back of the body waist tilts, the waist depression that has, and the buttocks broadness is smooth; Four limbs are solid, and often hind leg is higher than forelimb; 8～10 pairs of nipples.
According to for many years on the spot measurement result of Dingyuan pig breeding seminar of Agricultural University Of Anhui in Dingyuan pig conservation field, Dingyuan pig actual production performance is as follows:
Reproductive performance: 5 monthly ages of boar, sow 4-5 monthly age begin sexual maturity.11～15 of multiparity sow the number of live births, lactation power is high.How to oestrus in 7 days after the sow wean, non-return rate is more than 90%.
Growth performance: about piglet birth weight 0.8kg, 35 ages in days ablactation litter weight, 70～100kg.10-30kg stage average daily gain 350～400g, 30～90kg stage average daily gain, 200～300g.Body weight 80～the 110kg of sow, average body length, chest measurement, height, circumference of cannon bone are respectively 122.36 ± 15.73,102.69 ± 4.44,57.74 ± 4.55 and 17.55 ± 2.21cm; The body weight 90-120kg of adult boar.
Carcass quality: when fattening pig is butchered at 85kg, dressing percentage 65%, carcass lean meat percentage 40～45%, 6～7 intercostal thickness of backfats, 30～40mm, skin depth 6～10mm, fat content 3～4% between intramuscular.
New American Duroc pig
New American Duroc pig whole body is brownish red by hair, and is deep mixed.Ear is medium large, turns forward, and face is slightly recessed, and the body body is tall and thin, chest breadth and dark, and fleshiness, four limbs are sturdy solid, and hoof is black, carries on the back into little arch, and linea ventralis is straight.
Reproductive performance: sexual maturity is evening, and 6～7 monthly ages began to oestrus, and can breed after 8 monthly ages, and common 3 days of duration of estrus, the oestrous cycle is 21 days, 8～10 of the every tire farrowing of multiparity sow.
Grow-finish performance: birth weight 1.7kg, 28 age in days weaning weights, 8～9kg, 135～140 ages in days reach the 100kg body weight, and every weightening finish 1kg consumes manufactured feed 2.24kg.
Carcass quality: when fattening pig was butchered at 100kg, dressing percentage was 75%, and carcass lean meat percentage is 63%～66%; Thickness of backfat 11.5mm, eye muscle area 30.8cm
2Meat is good, without PSE and DFD meat.
Utilize Dingyuan pig sow and new American Duroc pig boar hybridization to cultivate the 1st generation sow core group, import and seed selection through 2 Dingyuan pig blood relationships again, when containing Dingyuan pig blood relationship 87.5% and duroc blood relationship 12.5%, implement traversed by 2 generations of seed selection, can form Dingyuan pig new lines, i.e. Huaihe River, new lines Anhui pig.With 1 generation sow core group, import and seed selection through 2 Duroc boars blood relationships again, when containing duroc blood relationship 87.5% and Dingyuan pig blood relationship 12.5%, implement traversed by 2 generations of seed selection, can form Dingyuan pig new lines, namely new lines restrains pig surely.It cultivates route as shown in Figure 4, and breeding method and process are as follows:
The first step: genetic recombination
With 60 of the excellent individual of high-quality, high yield, degeneration-resistant Anhui Native kind Dingyuan pig as maternal, hybridize as male parent with 12 of excellent individual that derive from different familys in the new American Duroc pig boar of external lean meat species kind, 5 sows of every boar breeding, the sow of selecting and remain in generation in institute postpartum forms the 1st sow core group from generation to generation.
The 1st from generation to generation selection of sow core group:
For the first time select and remain and will carry out when 28 ages in days weans: select by a hair black, body weight is greater than 4kg, more than 7 pairs of numbers of nipples reach, and rounded, the sow that physique is good; Wean is rear to the body weight of every pig growth of 90kg body weight stage record and the forage volume of consumption, in order to calculate its growth rate and feedstuff-meat ratio;
Select and remain for the second time and carry out when 4 monthly age: superseded growth rate is slow, the pig that physique is poor;
Select and remain for the third time and when 6 monthly age, carry out: measure 6 monthly age body weight, the thickness of backfat, the rear daily gain to the 90kg body weight stage of calculating wean, feedstuff-meat ratio, select that daily gain 500g is above, feedstuff-meat ratio less than 3.2: 1, during 90kg below the live body thickness of backfat 20mm, eye muscle area is greater than 25cm
2Sow reserve seed for planting, select and remain out the 1st from generation to generation 60 of sows participate in for the second time cross mating.
Second step: be group's differentiation seed selection
1, take the 1st from generation to generation the sow core group carry out grading up as maternal, Dingyuan pig boar as male parent, 12 of Dingyuan pig boars derive from 12 different familys, 5 sows of every boar breeding, in giving birth to offspring with molecular labeling and the multiple selection index method sow of selecting and remain, Huaihe River, called after Anhui pig, form the 2nd generation Huaihe River, Anhui pig sow core group, it contains 75% Dingyuan pig blood lineage, 25% duroc blood lineage;
Carry out grading up take the 1st generation sow core group as maternal, new American Duroc pig boar as male parent, Duroc boars derives from 12 different familys, 5 sows of every boar breeding, in giving birth to offspring with molecular labeling and the multiple selection index method sow of selecting and remain, called after restrains pig surely, form the 2nd and surely restrain pig sow core group from generation to generation, it contains 75% duroc blood lineage, 25% Dingyuan pig blood lineage;
The 2nd generation Huaihe River, Anhui pig sow core group and the 2nd concrete seed selection that surely restrains pig sow core group from generation to generation operate as follows:
Huaihe River, Anhui pig sow carries out selecting and remain from birth to weaning period first time, should be according to the testing result of molecular genetic marker, and the MC1R genotype of selecting and remain is AA (by a mao black), the THRSP genotype is AA and GA; The wean of 28 ages in days the time is carried out selecting and remain second time, requires that the bodily form is good, physique is good, physically well develops, and calculates the wean multiple selection index according to wean individual weight and the young number of brood work again, determines the individuality of reserving seed for planting according to the multiple selection index that weans is descending; Select and remain for the third time during 4 monthly age, depauperation, the pinto pig of quilt hair are eliminated; Again according to wean multiple selection index, 4 monthly age individual weights and the long 4 monthly age multiple selection indexs that calculate of body, determine the individuality of reserving seed for planting according to 4 monthly age multiple selection indexs are descending.
Surely restrain the pig sow from birth to weaning period, carry out selecting and remain first time, should be according to the testing result of molecular genetic marker, to select and remain and select the MC1R genotype be GG (by mao brown), the THRSP genotype is AA and GA; The wean of 28 ages in days the time is carried out selecting and remain second time, require the bodily form, physique good, physically well develop, calculate the wean multiple selection index according to wean individual weight and the young number of brood work again, determine the individuality of reserving seed for planting according to the multiple selection index that weans is descending; Select and remain for the third time during 4 monthly age, depauperation, the pinto pig of quilt hair are eliminated; Again according to wean multiple selection index, 4 monthly age individual weights and the long 4 monthly age multiple selection indexs of formulating of body, determine the individuality of reserving seed for planting according to 4 monthly age multiple selection indexs are descending.
2 strains are carried out respectively the 4th tight choosing when 6 monthly age.Calculate 6 monthly age multiple selection indexs according to the thickness of backfat that 4 monthly age multiple selection indexs, 6 monthly age individual weights and B ultrasonic record, select according to 6 the descending of monthly age multiple selection index; Replacement gilt carries out intensity with 3: 1 ratio to be selected and remain, and 60 sows of finally selecting and remain form the core group of this generation and participate in breeding each strain.
The genotypic detection method of MC1R:
Extract determined pig genomic DNA, take the genomic DNA that extracts as template, MRAG-S and MRAG-A are that primer carries out pcr amplification, and the nucleotide sequence of primer MRAG-S and MRAG-A is as follows:
MRAG-S：GCGGGACGATGCCTGTGCTT，
MRAG-A：GGTAGCGCAGCGCGTAGAAGAT
Pcr amplification system: total system 25 μ L, upstream and downstream primer each 1.0 μ L (10 μ M/L), 10 * PCR buffer, 2.5 μ L, dNTPs 2.0 μ L (2.5mM/L), Taq archaeal dna polymerase 0.5 μ L (5U/ μ L), dna profiling 1.0 μ L (50ng/ μ L), ddH
2O 17 μ L;
The pcr amplification condition: 94 ℃ of denaturation 5min, 94 ℃ of 30s, the 58 ℃ of 30s that anneal, 72 ℃ of 1min, 35 circulations, 72 ℃ are extended 10min, 4 ℃ of preservations;
With NspI digestion with restriction enzyme PCR product;
15 μ L endonuclease reaction systems: PCR product 6 μ L, NspI restriction endonuclease 0.5 μ L (10U/ μ L), 10 * buffer, 1.5 μ L, ddH
2O 7 μ L;
Enzyme is cut 12h, and 3% agarose gel electrophoresis detects, and takes pictures and differentiates genotype with the gel imaging instrument;
NspI enzyme cutting type, the black wool pig is homozygous to be AA, and brown hair is homozygous to be GG, and the heterozygous of black wool pig is GA.See Fig. 1;
The genotypic detection method of THRSP:
Extract determined pig genomic DNA; Take the genomic DNA that extracts as template, TP2-S and TP2-A are that primer carries out pcr amplification, and the nucleotide sequence of primer TP2-S and TP2-A is as follows:
TP2-S：TACCCCAAGAACTGCCTGCTGA
TP2-A：GGTGGAACTGGGCTTCTAGGTC
Pcr amplification system: total system 25 μ L, upstream and downstream primer each 1.0 μ L (10 μ M/L), 10 * PCR buffer, 2.5 μ L, dNTPs 2.0 μ L (2.5mM/L), Taq archaeal dna polymerase 0.5 μ L (5U/ μ L), dna profiling 1.0 μ L (50ng/ μ L), ddH
2O 17 μ L; Pcr amplification condition: 94 ℃ of denaturation 5min;
The pcr amplification program: 94 ℃ of 30s, the 58 ℃ of 30s that anneal, 72 ℃ of 1min, 35 circulations, 72 ℃ are extended 10min, 4 ℃ of preservations;
With MvaI and PvuII digestion with restriction enzyme PCR product;
The reaction system that 5 μ L enzymes are cut: PCR product 6 μ L, restriction endonuclease 0.5 μ L (10U/ μ L), 10 * buffer, 1.5 μ L, ddH
2O7 μ L;
Enzyme is cut 12h, and 3% agarose gel electrophoresis detects, and takes pictures and differentiates genotype with the gel imaging instrument;
Cut the PCR products of dissimilar pigs with the PvuII enzyme, obtain 3 kinds of genotype, wherein select and remain AA and GA genotype are seen Fig. 2; Cut the PCR products of dissimilar pigs with the MvaI enzyme, obtain 3 kinds of genotype, wherein select and remain AA and G.A genotype are seen Fig. 3;
The different phase multiple selection index is as follows:
Wean multiple selection index=wean individual weight * 40%/weanling weight average+young the number of brood work * 60%/young number average value of work.
The long average of long * 30%/body of 4 monthly ages multiple selection index=wean multiple selection index * 20%+4 monthly age individual weight * 50%/body weight average+body.
The individual 6 monthly age individual weights of 6 monthly age multiple selection indexs=4 monthly ages multiple selection index * 20%+ * 50%/body weight average+6～7 ribbed back fat thickness * 30%/6～7 ribbed back fat thickness averages.
2, be group's grading purifying and selection
Take the 2nd from generation to generation 60 of Huaihe River, Anhui pig sow core groups for female parent, carry out grading up take 12 of Dingyuan pig boars as male parent again, Dingyuan pig boar derives from 12 different familys, the 3rd Huaihe River, the Anhui pig core group from generation to generation of in giving birth to offspring, selecting and remain, it contains 87.5% Dingyuan pig blood lineage, 12.5% duroc blood lineage;
Surely restrain from generation to generation 60 of pig sow core groups for female parent take the 2nd, carry out grading up take 12 of duroc boars as male parent again, Duroc boars derives from 12 different familys, in giving birth to offspring, select and remain and the 3rd surely restrain from generation to generation the pig core group, it contains 87.5% duroc blood lineage, 12.5% Dingyuan pig blood lineage.
The 3rd from generation to generation Huaihe River, Anhui pig core group male and female pig and the 3rd surely restrain from generation to generation pig core group male and female pig the first step select and remain, mark THRSP gene, the genotype of selecting and remain is the pig of AA and GA, 2nd, 3,4 steps selected and remain and surely restrain the 2nd, 3,4 of pig sow core group and select and remain identical with the 2nd generation Huaihe River, Anhui pig sow core group, the 2nd generation, gained male and female pig carries out intensity with the ratio of 3: 1 (quantity) respectively and selects and remain, namely in the male and female swinery, be and go out first 1 in 3, select and remain 100 of sows, 20 of boars.
The 3rd step: traversed by group expanding
This step traversed by group expanding is avoided in the inbred situation in each new lines inside exactly, the breeding that utilizes same from generation to generation male and female pig to carry out, and enlarge Population.2 new lines adopt respectively colony's locking breeding and seed selection from generation to generation since the 3rd; The method that is aided with molecular marker assisted selection with colony's subculture seed selection is carried out the traversed by group expanding of 2 generations, 1 year generation; Matching method is this generation boar and sow mating, each 100 of sow of selecting and remain from generation to generation, and 20 of boars are through 2 core groups of tentatively setting up respectively new lines Anhui Huaihe River pig and Ding Ke pig from generation to generation.
Each selects and remain traversed by for the 1st, 2,3,4 steps of pig core group and intensity selects and remain that from generation to generation Huaihe River, Anhui pig core group, the 3rd the 1st, 2,3,4 steps of surely restraining the pig core group from generation to generation select and remain and intensity is selected and remain identical with the 3rd.
The performance indications of Huaihe River, new lines Anhui pig are:
Reproductive performance: the highest farrowing of pig core group multiparity sow of Huaihe River, Anhui reaches 21, and average nest litter size reaches 12.99, and average nest is lived young several 12.50.
Growth rate: it is 180 ages in days that the market pig body weight reaches time of 90 kilograms, and the price of deed is 3.35: 1.
Trunk and meat quality: lean meat percentage is 52%～56%, and intramuscular fat is 3.3%～4.0%, and the nothing yellowish pink is pale, quality is soft, tangent plane oozes out and dark, strong, dry meat, marble grain scoring 3.3～3.5, yellowish pink scoring 3.2～3.6.
The performance indications that new lines restrains pig surely are:
Reproductive performance: surely restrain pig core group multiparity sow and be up to 19, average nest litter size reaches 11.58, and nest is lived young several 11.36.
Growth rate: it is 145 ages in days that the market pig body weight reaches time of 100 kilograms, and the price of deed is 2.78: 1;
Trunk and meat quality: lean meat percentage is 64%～68%, and intramuscular fat is 3.1%～3.5%, and the nothing yellowish pink is pale, quality is soft, tangent plane oozes out and dark, strong, dry meat, marble grain scoring 3.0～3.5, yellowish pink scoring 3.2～3.5.
Above-described embodiment is described preferred embodiment of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (5)
1. the breeding method of a Dingyuan pig new lines is characterized in that may further comprise the steps:
Phase I:
With the excellent individual of high-quality, high yield, degeneration-resistant Anhui Native kind Dingyuan pig as female parent, hybridize as male parent with the excellent individual that derives from different familys in the new American Duroc pig boar of external lean meat species kind, the sow of selecting and remain in generation in institute postpartum forms the 1st sow core group from generation to generation;
Second stage:
Take the 1st from generation to generation the sow core group carry out grading up as maternal, Dingyuan pig boar as male parent, Dingyuan pig boar derives from different familys, in giving birth to offspring with molecular labeling and the multiple selection index method sow of selecting and remain, Huaihe River, called after Anhui pig, form the 2nd generation Huaihe River, Anhui pig sow core group, it contains 75% Dingyuan pig blood lineage, 25% duroc blood lineage;
Carry out grading up take the 1st generation sow core group as maternal, new American Duroc pig boar as male parent, Duroc boars derives from different familys, in giving birth to offspring with molecular labeling and the multiple selection index method sow of selecting and remain, called after restrains pig surely, form the 2nd and surely restrain pig sow core group from generation to generation, it contains 75% duroc blood lineage, 25% Dingyuan pig blood lineage;
Phase III:
Take the 2nd generation Huaihe River, Anhui pig sow core group as maternal, carry out grading up take Dingyuan pig boar as male parent again, Dingyuan pig boar derives from different familys, in giving birth to offspring with molecular labeling and multiple selection index method select and remain boar and sow, form the 3rd generation Huaihe River, Anhui pig core group, it contains 87.5% Dingyuan pig blood lineage, 12.5% duroc blood lineage;
Surely restrain from generation to generation pig sow core group as maternal take the 2nd, carry out grading up take new American Duroc pig pig boar as male parent again, Duroc boars derives from different familys, in giving birth to offspring with molecular labeling and multiple selection index method select and remain boar and sow, form the 3rd and surely restrain the pig core group from generation to generation, it contains 87.5% duroc blood lineage, 12.5% Dingyuan pig blood lineage;
The quadravalence section:
Respectively with the male and female pig mating in the 3rd generation Huaihe River, the Anhui pig core group, with the 3rd male and female pig mating that surely restrains from generation to generation in the pig core group, adopt respectively from generation to generation colony's locking breeding and seed selection since the 3rd, carry out the traversed by of 2 ~ 3 generations, traversed by each for pig in molecular labeling and multiple selection index method select and remain boar and sow, obtain traversed by respectively for the pig core group, same generation boar and sow mating, each select and remain from generation to generation sow and boar, after carrying out the traversed by of 2 ~ 3 generations, obtain new lines Anhui Huaihe River pig and new lines and surely restrain pig, it is Dingyuan pig new lines.
2. the breeding method of Dingyuan pig new lines according to claim 1, it is characterized in that the described the 1st from generation to generation the selection of sow core group be:
For the first time select and remain and will carry out when 28 ages in days weans: select by a hair black, body weight is greater than 4kg, more than 7 pairs of numbers of nipples reach, and rounded, the sow that physique is good;
Select and remain for the second time and carry out when 4 monthly age: superseded growth rate is slow, the pig that physique is poor;
Select and remain for the third time and when 6 monthly age, carry out: measure 6 monthly age body weight, the thickness of backfat, the rear daily gain to the 90kg body weight stage of calculating wean, feedstuff-meat ratio, select that daily gain 500g is above, below the live body thickness of backfat 20mm, eye muscle area is greater than 25cm during less than 3.2:1,90kg for feedstuff-meat ratio
2Sow reserve seed for planting.
3. the breeding method of Dingyuan pig new lines according to claim 1, it is characterized in that the described the 2nd from generation to generation Huaihe River, Anhui pig sow core group and the 2nd selection that surely restrains pig sow core group from generation to generation be:
Huaihe River, Anhui pig carries out selecting and remain from birth to weaning period the first time, should be according to the testing result of molecular genetic marker, and the MC1R genotype of selecting and remain is AA, namely by hair black, the THRSP genotype is AA and GA; The wean of 28 ages in days the time is carried out selecting and remain second time, requires that the bodily form is good, physique is good, physically well develops, and calculates the wean multiple selection index according to wean individual weight and the young number of brood work again, determines the individuality of reserving seed for planting according to the multiple selection index that weans is descending; Select and remain for the third time during 4 monthly age, depauperation, the pinto pig of quilt hair are eliminated; Again according to wean multiple selection index, 4 monthly age individual weights and the long 4 monthly age multiple selection indexs that calculate of body, determine the individuality of reserving seed for planting according to 4 monthly age multiple selection indexs are descending; Carry out the 4th tight choosing when 6 monthly age, calculate 6 monthly age multiple selection indexs according to the thickness of backfat that 4 monthly age multiple selection indexs, 6 monthly age individual weights and B ultrasonic record, the monthly age multiple selection index is descending selects according to 6;
Surely restrain pig from birth to weaning period, carry out selecting and remain the first time, should be according to the testing result of molecular genetic marker, it is brown by hair to select and remain, and the THRSP genotype is AA and GA; The wean of 28 ages in days the time is carried out selecting and remain second time, requires that the bodily form is good, physique is good, physically well develops, and calculates the wean multiple selection index according to wean individual weight and the young number of brood work again, determines the individuality of reserving seed for planting according to the multiple selection index that weans is descending; Select and remain for the third time during 4 monthly age, depauperation, the pinto pig of quilt hair are eliminated; Long according to wean multiple selection index, 4 monthly age individual weights and body again, calculate 4 monthly age multiple selection indexs, foundation 4 monthly age multiple selection indexs are descending determines the individuality of reserving seed for planting; Carry out the 4th tight choosing when 6 monthly age, calculate 6 monthly age multiple selection indexs according to the thickness of backfat that 4 monthly age multiple selection indexs, 6 monthly age individual weights and B ultrasonic record, the monthly age multiple selection index is descending selects according to 6;
From through above-mentioned select and remain for four times the 2nd from generation to generation Huaihe River, Anhui pig sow and the 2nd surely restrain the replacement gilt of pig sow all quantitative proportion with 3:1 from generation to generation and carry out intensity and select and remain, be 3 ratios of selecting one among the sow group, obtain the 2nd generation Huaihe River, Anhui pig sow core group and the 2nd and surely restrain pig sow core group from generation to generation.
4. the breeding method of Dingyuan pig new lines according to claim 1, it is characterized in that the described the 3rd surely restrain from generation to generation pig core group, the 3rd from generation to generation Huaihe River, Anhui pig core group and traversed by each for pig core group selection be:
Piglet carries out selecting and remain from birth to weaning period first time, should be according to the testing result of molecular genetic marker, and selection THRSP genotype is AA and GA;
Carry out selecting and remain the second time during wean of piglet 28 ages in days, heavy and the young number calculating of the brood work wean multiple selection index according to weaned piglet, determine the individuality of reserving seed for planting according to the wean multiple selection index is descending, eliminate simultaneously that physique is poor, depauperation and by the pinto pig of hair-videotape;
When 4 monthly age, select and remain for the third time, according to wean multiple selection index, 4 monthly age individual weights and the long 4 monthly age multiple selection indexs that calculate of body, determine the individuality of reserving seed for planting according to 4 monthly age multiple selection indexs are descending, eliminate simultaneously depauperation and by the pinto pig of hair;
6 monthly ages carried out the 4th time and select and remain, and calculated 6 monthly age multiple selection indexs according to 4 monthly age multiple selection indexs, 6 monthly age individual weights and the thickness of backfat, determined the individuality of reserving seed for planting according to 6 monthly age multiple selection index sizes, eliminated simultaneously hypogenetic pig;
From the replacement gilt through above-mentioned male and female pig of selecting and remain for four times, the male and female pig all carries out intensity with the ratio of 3:1 and selects and remain, be to be 3 ratios of selecting one in the male and female swinery, obtain the 3rd from generation to generation Huaihe River, Anhui pig core group, the 3rd surely restrain from generation to generation pig core group or traversed by each for the pig core group.
5. it is characterized in that according to claim 1 and 2 or the breeding method of 3 or 4 described Dingyuan pig new lines:
Generation interval be the 1st from generation to generation sow core group, the 2nd from generation to generation Huaihe River, Anhui pig sow core group, the 2nd surely restrain from generation to generation pig sow core group, the 3rd from generation to generation Huaihe River, Anhui pig core group, the 3rd surely restrain pig core group and each time interval of successively breeding for the pig core group of traversed by from generation to generation, Huaihe River, Anhui pig is in 5 ~ 7 monthly ages, body weight 80-90kg, surely restrain pig and when 6 ~ 8 monthly ages, body weight 100kg ~ 120kg, concentrate breeding, should avoid full sibs and half-sib mating during breeding; 2 respectively select and remain through 4 times for pig from generation to generation and later on, concentrate breeding again; The piglet of primiparity is reserved seed for planting, 1 year generation;
The auxiliary seed choosing method of molecular labeling is: the MC1R gene is selected hair color, and the THRSP gene is selected meat, adopts the ear tissue sample when piglet is born, and returns the laboratory and carries out Markers for Detection, selects to reserve seed for planting and eliminate according to the genotype that molecular labeling goes out;
The genotypic detection method of MC1R:
Extract determined pig genomic DNA, take the genomic DNA that extracts as template, MRAG-S and MRAG-A are that primer carries out pcr amplification, and the nucleotide sequence of primer MRAG-S and MRAG-A is as follows:
MRAG-S:GCGGGACGATGCCTGTGCTT,
MRAG-A:GGTAGCGCAGCGCGTAGAAGAT
Pcr amplification;
With NspI digestion with restriction enzyme PCR product;
Agarose gel electrophoresis detects, and takes pictures and differentiates genotype with the gel imaging instrument;
NspI enzyme cutting type, the black wool pig is homozygous to be AA, and brown hair is homozygous to be GG, and the heterozygous of black wool pig is GA;
The genotypic detection method of THRSP:
Extract determined pig genomic DNA; Take the genomic DNA that extracts as template, TP2-S and TP2-A are that primer carries out pcr amplification, and the nucleotide sequence of primer TP2-S and TP2-A is as follows:
TP2-S:TACCCCAAGAACTGCCTGCTGA
TP2-A:GGTGGAACTGGGCTTCTAGGTC
Pcr amplification;
With Mva I and Pvu II digestion with restriction enzyme PCR product;
Agarose gel electrophoresis detects, and takes pictures and differentiates genotype with the gel imaging instrument;
Cut the PCR products of dissimilar pigs with Pvu II enzyme, obtain 3 kinds of genotype, wherein select and remain AA and GA genotype; Cut the PCR products of dissimilar pigs with Mva I enzyme, obtain 3 kinds of genotype, wherein select and remain AA and GA genotype;
The different phase multiple selection index is as follows:
Wean multiple selection index=wean individual weight * 40%/weanling weight average+young the number of brood work * 60%/young number average value of work,
The long average of long * 30%/body of 4 monthly ages multiple selection index=wean multiple selection index * 20%+4 monthly age individual weight * 50%/4 monthly age body weight average+body,
The individual 6 monthly age individual weights of 6 monthly age multiple selection indexs=4 monthly ages multiple selection index * 20%+ * 50%/6 monthly age body weight average+6 ~ 7 ribbed back fat thickness * 30%/6 ~ 7 ribbed back fat thickness averages.
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