CN102090267A

CN102090267A - Method for cultivating mulberry twig glossy ganoderma

Info

Publication number: CN102090267A
Application number: CN 201010585888
Authority: CN
Inventors: 马锋军
Original assignee: YANGCHENG KEHUI INSTITUTE OF MICROBIOLOGY
Current assignee: YANGCHENG KEHUI INSTITUTE OF MICROBIOLOGY
Priority date: 2010-12-14
Filing date: 2010-12-14
Publication date: 2011-06-15
Anticipated expiration: 2030-12-14
Also published as: CN102090267B

Abstract

The invention discloses a method for cultivating mulberry twig glossy ganoderma. The method comprises the following steps of: (A) bagging; (B) sterilizing and inoculating; (C) culturing; (D) inducing primordia; (E) managing mushroom culturing; (F) grafting; (G) performing aeration drying; and (H) spraying paint and boxing. The mulberry twig glossy ganoderma cultivated with the method provided by the invention has the characteristics of attractive appearance, good ornamental effect and high reserve value, and is beneficial to the expansion of virtuous circle of the silkworm industry; and the planting yield of ganoderma is greatly increased.

Description

The cultivation method of a kind of ramulus mori glossy ganoderma

Technical field

The present invention relates to a kind of plant cultivation method, relate in particular to the cultivation method of a kind of ramulus mori glossy ganoderma.

Background technology

Glossy ganoderma belongs to Basidiomycetes, Aphyllophorales, Polyporaceae, Ganoderma.Glossy ganoderma just is considered to lucky, valuable, fine, long-lived symbol since ancient times, the title that celestial grass, refreshing grass, auspicious grass are just arranged from ancient times, representing auspicious sign, longevity, good fortune expensive, pharmacological research shows, glossy ganoderma also have protect the liver, detoxifcation, cardiac stimulant, calm the nerves, functions such as beneficial lung qi, sharp joint, the direct-view for a long time of chinese tradition medical science for strengthening by means of tonics, consolidate the precious Chinese herbal medicine of setting upright.

In recent years, glossy ganoderma begins to enter people's life as decorative articles, and glossy ganoderma cooperates various Chinese herbal medicines, flowers and plants, merges modern's health, fashion, attractive in appearance, novel psychological needs, integrates health and sight, artistry.

Though the present disclosed cultivation method that multiple glossy ganoderma is arranged is not seen the cultivation method of public use ramulus mori growth glossy ganoderma.Do not see by control light and CO yet ₂Come cultivation method that glossy ganoderma growth is controlled.

Summary of the invention

The present invention is intended to solve the technical problem of above-mentioned proposition.

The invention provides the cultivation method of a kind of ramulus mori glossy ganoderma, comprise the steps: A) pack, mark selects 1 part of 69 parts of ramulus mori section, 10 parts of wood chips, 20 parts in wheat bran, gypsum as raw material by weight, the ramulus mori section is pressed into the pond soaks, make it water content and reach 60%, with wood chip, wheat bran, gypsum blending back poach, material: water=1: 1.1, with the raw material cultivation bag of packing into; B) disinfection inoculation, pack is controlled in the 6h at interval with sterilization time, normal-pressure sterilization, cooling naturally of sterilization back is waited to expect to begin inoculation about temperature drop to 28 ℃, during inoculation bacterial classification is broken into pieces with one's fingers, shakes after the inoculation to plant to bacterial classification to be evenly distributed on media surface; C) cultivate, move into culturing room with connecting the bacterium bag of planting, temperature remains on 22-26 ℃ between bacteria developing period, air relative temperature 55-65%, and with fresh air, half-light is cultured to basic purseful; D) urge flower bud to take place, after the mycelia purseful, bacterium bag temperature is reduced to 24 ℃, kept 7-10 days; With bag temperature rise to 28 ℃, keep relative air humidity 90%, sufficient oxygen and scattered light fruit body primordium occurs to the composts or fertilisers of cultivating surface; Keep fruiting field temperature 24-28 ℃ this moment, relative air humidity 90%-95%, and sufficient oxygen and scattered light are repeatedly sprayed water on a small quantity; E) educate the mushroom management, in the sporophore growth process, temperature 24-28 ℃, relative air humidity 85%-95%, light and ventilation are adjusted according to growing state and shaping needs; When high, the regulation and control light source allows fruit body be in the low light level to the group true length, reduces ventilation number, makes CO2 content between 0.1%-0.3% to 3cm, in each ventilation time control 30min, and every day 1-2 time; During the long 8-10cm of mushroom handle, increase illumination and ventilation, make fruit body begin to open up sheet; F) grafting; G) aeration-drying; H) spray paint, adorn box.

The ramulus mori glossy ganoderma of adopting method cultivation provided by the invention to obtain has characteristics attractive in appearance, that sight is high, reserve value is big, and helps extending the benign cycle of silkworm industry, significantly improves the income of glossy ganoderma plantation.

Description of drawings

Fig. 1 is the flow chart of ramulus mori ganoderma lucidum cultivation method provided by the invention.

Embodiment

1, planting material and prescription

1.1 cultivar: separating obtained by boa river Wild ganoderma, 25-35 ℃ of fruiting temperature, brownish red, many Dan Sheng, body is just justified greatly.

1.2 planting material: give birth to Ramulus Mori then, diameter 0.5-1.5cm is cut into the 20cm section, and other has wood fragments bits, wheat bran, gypsum etc.Cultivation bag adopts the conventional Polythene Bag of folding length 18cm * 38cm * 0.04cm.

Plant prescription 1.3 plant: ramulus mori section 69%, wood chip 10%, wheat bran 20% by weight percentage, gypsum 1%.

2, plantation technology

2.1 bacterial classification production

2.1.1 female the kind: adopt the PDA medium, 0.11Mpa, sterilization 30min treats that slant medium is cooled to the inoculation of anhydrous back, puts 24 ℃ of insulating boxs and cultivates 14 days.Full packages, stand-by.

2.1.2 original seed: wood chip 78%, wheat bran 20%, gypsum 1%, sugar 1% by weight percentage.Material: water=1: 1.1.With 15cm * 28cm * 0.05cm knuckle Polypropylene Bag pack, autoclaving, 0.15Mpa, 2.5h, cooling inoculation respectively at 10 days, 15 days and 20 days, is checked the fungi pollution situation of sending out after the inoculation.24 ℃ of cultivation temperature, 29 days basic pursefuls, stand-by.

2.1.3 cultivated species: make same original seed.

2.2 cultivation bag is made:

Prescription and used cultivation bag see 1.1 and 1.2, pack preceding 1 day, the ramulus mori section is pressed into the pond soaks, and make it water content and reach 60%.With blending back poaches such as wood chip, wheat bran, gypsum, material: water=1: 1.1.At the bottom of bag, spread earlier the particle of the about 2cm of a bed thickness during pack, then the ramulus mori section is packed into, and tamp space between the ramulus mori section, repave the thick particle compacting of one deck 2cm with particle.Even consolidation is wanted in charging, and charge level is smooth, and collar height is apart from charge level 3cm, and tampon is mentioned and do not dropped.

2.3 disinfection inoculation

Pack is controlled in the 6h with sterilization time at interval, normal-pressure sterilization, 100 ℃ of material temperature, 24h, sterilization back is cooling naturally, waits to expect that temperature drop to 28 ℃ begins to inoculate, every disinfections such as inoculating hood, bacterial classification, inoculating tool and staff's clothing apparatus are carried out in strictness before the inoculation, break into bacterial classification into pieces with one's fingers the iblet size during inoculation, about the every bag of amount of sowing 20g, shake kind to bacterial classification after the inoculation and be evenly distributed on media surface.

2.4 cultivate

Connect the bacterium bag of planting and move into culturing room, temperature remains on 22-26 ℃ between bacteria developing period, and air relative temperature 55-65% is with fresh air, and half-light is cultivated, basic purseful in the time of 35 days.

2.5 urging flower bud takes place

2.5.1 after the mycelia purseful, bacterium bag temperature is reduced to 24 ℃, 7-10 days.

2.5.210 with bag temperature rise to 28 ℃, keep relative air humidity 90% after it, sufficient oxygen and scattered light.

2.5.33-7 my god, the white projection of soybean grain size, i.e. fruit body primordium appear in the composts or fertilisers of cultivating surface, keep fruiting field temperature 24-28 ℃ this moment, relative air humidity 90%-95%, sufficient oxygen and scattered light, water spray will be on a small quantity repeatedly, and spray space and ground should not be sprayed on water on the young flower bud.

2.6 educate the mushroom management

2.6.1 in the sporophore growth process, temperature 24-28 ℃, relative air humidity 85%-95%, light and ventilation are adjusted according to growing state and shaping needs.

When high, 2.6.2 the regulation and control light source allows fruit body be in the low light level to the group true length, reduces ventilation number, makes CO2 content between 0.1%-0.3% to 3cm, in each ventilation time control 30min, and every day 1-2 time.Do to make the thick shape of mushroom handle like this, and can occur than multi-branched.

2.6.3 when the long 8-10cm of mushroom handle, increase illumination and ventilation, the demand that restore normal growth allows fruit body begin to open up sheet.

2.6.4 select the grafting for the first time of good the carrying out of nature moulding, on white growing point, open " V " font mouth with sharp sword, get the fruit body of growing, base portion is whittled into and scion that " V " word mouth matches, insert and, can heal in 2-3 days in conjunction with consolidation.

2.6.5 grafting for the first time can be carried out the grafting second time about 7-10 days, method is the same.

2.6.6 after grafting was finished for the third time, when the fruit body blade launches fully, marginal growing-point reddened fully, will carry out spore when launching, and shifted out mushroom producing culture chamber natural air drying.During drying note dustproof and counter tide.When air-dry between temperature 20-30 ℃, the big ventilation of 24h.

3, spray paint, adorn box

3.1 fruit body is used clean soft cloth after doing, banister brush is cleaned the fruit body surface attachments, preserves the longer time for making it, and is with environment-friendly type varnish spraying 3 times, every all over 2 days at interval.

3.2 remove 2/3rds culture bag, plant in the box, fill with the seven color rubbles of cleaning, so far the glossy ganoderma of a basin beautiful design has just become.

Should be noted that in the incubation that in the process of growth of fruit body the low light level can constantly extend by stimulon entity shank, does not even break up cap.And CO2 content is when reaching 0.5%-1%, and the fruit body branch is more, as deer horn.In the sporophore growth process, utilize this two factors fully flexibly, make the fruit body teratogenesis, so just can obtain strange shape ganoderma lucidum fruitbody.Tight connecting is wanted in the junction during grafting, and the scion individuality is excessive, but supplemental support.Water spray should not be sprayed directly on to water the mushroom face during humidification, otherwise that blade surface easily has is water stain.The tall and big ganoderma lucidum fruitbody of the big more easy more acquisition of medium.

Claims

1. the cultivation method of a ramulus mori glossy ganoderma comprises the steps:

A) pack

Mark selects 1 part of 69 parts of ramulus mori section, 10 parts of wood chips, 20 parts in wheat bran, gypsum as raw material by weight, the ramulus mori section is pressed into the pond soaks, make it water content and reach 60%, with wood chip, wheat bran, gypsum blending back poach, material: water=1: 1.1, with the raw material cultivation bag of packing into;

B) disinfection inoculation

Pack is controlled in the 6h at interval with sterilization time, normal-pressure sterilization, and cooling naturally of sterilization back is waited to expect to begin inoculation about temperature drop to 28 ℃, during inoculation bacterial classification is broken into pieces with one's fingers, shakes after the inoculation to plant to bacterial classification to be evenly distributed on media surface;

C) cultivate

Move into culturing room with connecting the bacterium bag of planting, temperature remains on 22-26 ℃ between bacteria developing period, air relative temperature 55-65%, and with fresh air, half-light is cultured to basic purseful;

D) urge flower bud to take place

After the mycelia purseful, bacterium bag temperature is reduced to 24 ℃, kept 7-10 days;

With bag temperature rise to 28 ℃, keep relative air humidity 90%, sufficient oxygen and scattered light fruit body primordium occurs to the composts or fertilisers of cultivating surface;

Keep fruiting field temperature 24-28 ℃ this moment, relative air humidity 90%-95%, and sufficient oxygen and scattered light are repeatedly sprayed water on a small quantity;

E) educate the mushroom management

In the sporophore growth process, temperature 24-28 ℃, relative air humidity 85%-95%, light and ventilation are adjusted according to growing state and shaping needs;

When high, the regulation and control light source allows fruit body be in the low light level to the group true length, reduces ventilation number, makes CO2 content between 0.1%-0.3% to 3cm, in each ventilation time control 30min, and every day 1-2 time;

During the long 8-10cm of mushroom handle, increase illumination and ventilation, make fruit body begin to open up sheet;

F) grafting

G) aeration-drying

H) spray paint, adorn box.

2. the method for claim 1 is spread one deck particle earlier at the bottom of bag when it is characterized in that packing, and then the ramulus mori section is packed into, and tamps space between the ramulus mori section with particle, repaves the compacting of one deck particle.

3. the method for claim 1 is characterized in that the water spray among the step D should be sprayed on space and ground, water is not sprayed on the young flower bud.

4. the method for claim 1, it is characterized in that step F comprises three graftings, grafting is for the first time opened " V " font mouth with sharp sword on white growing point, get the fruit body of growing, base portion is whittled into the scion that matches with " V " word mouth, insert and, can heal in 2-3 days in conjunction with consolidation; For the first time can carry out grafting second time in about 7-10 days after the grafting, method is identical with the first time; Carry out grafting for the third time then.

5. the method for claim 1, the condition that it is characterized in that step G are dustproof and moistureproof, air-dry temperature between 20-30 ℃, ventilation 24h.

6. the method for claim 1 is characterized in that described cultivation bag adopts the Polythene Bag of folding length 18cm * 38cm * 0.04cm.