CN102057864A

CN102057864A - Potato cloning gene mutation, chromosomal variation and breeding technology

Info

Publication number: CN102057864A
Application number: CN2009101334085A
Authority: CN
Inventors: 杜林东
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-03-31
Filing date: 2009-03-31
Publication date: 2011-05-18

Abstract

The invention provides a novel potato fine variety breeding technology. By the technology, any potato variety can be bred by the novel method, which means to breed next generation by a method of converting yin and yang, so the chromosome and mitosis of the potato are varied (namely, cloning gene mutation), and each bred potato can be used as a fine potato variety. The invention comprises two particular implementation schemes; the method is simple and convenient, and is easy to understand, so peasants can breed fine varieties by the breeding technology to achieve the aim of self breeding and self seedling.

Description

Potato vegetative propagation gene mutation, chromosomal variation, breeding technique

Technical field:

I teach the method for setting seeds with breeding potato such as K299 without pollen, after drug disinfection, carry out transformation between yin and yang in 1 year, am the chromosome and the mitosis variation of regeneration strain system, cause gene mutation.Plantation in 1 year becomes 6 to raise variety.Be characterized in: form has nothing in common with each other; Wherein the crust adularescent is purpuric, has azuryly; White skin plain boiled pork is arranged, purple Beijing opera meat, purple skin plain boiled pork, the yellow meat of Calusena lansium; Eye has the blood-shot eye illness nest, blue eye socket, have to open and spend or open blue flower in vain.Terrestrial stem is viridescent, and is purpuric, and conversion is precocious, late-maturing two strains.The method of this breeding, the potato starch content height, quality better, the output height that bring out; Antiviral extremely strong, and storage endurance, can also allow the sincere kind of withering, shine new life and vigor, slough virus.

Background technology:

Cultivate genetically modified crops and raise transgenic animal, genetically modified food, transgenosis medicine with transgenic technology on the science at present, so far also do not find asexual gene mutation chromosome and mitosis variation technology, so the asexual gene mutation technology of the potato that I invented is beyond example.Organism, chromosome and mitosis variation technology have been opened up new development space on science, for biological development has had new starting point, broken biologically sexual single saying of breeding variation.

Summary of the invention:

Each potato that potato breeding, gene mutation method bring out can be used as good potato kind.

Embodiment:

After good variety selection after the variation gone out, individual plant is separated, coming year plantation, or, wherein best kind is continued to breed new offspring with the way of transformation between yin and yang.

Claims

1. 1. with the original seed of No. 3, potato breeding Gansu Province potato of select, after soaking 3 hours with 0.01% iodophor solution, be placed on the heated kang in pasture on March, temperature is between 6-10 ℃.Evening or cloudy day quilt on the potato upper cover when daytime, the sun came out, are opened quilt, allow light inject airing from the window that glass is arranged.Grew at eye in about 20 days, asexual gene mutation, the living potato of dream of chromosome and mitosis variation after the potato piece takes off, is placed in the sterile cellar for storing things when the living potato of dream is grown up to 10 to 30 grams, shuts Jiao Men, in unglazed, temperature between 3-4 ℃.Humidity is in 90% environment after the dormancy 380 days; In late May, second plantation.September 20 gathered, gathered in the crops preceding about 10 days, roll seedling earlier or cut seedling, make the potato skin aging, the potato piece of results no longer is No. 3, Gansu Province potato, and become the new potato kind of various gene mutation, individual plant is reserved seed for planting, use as breeding in should depositing respectively, preserve and storing, temperature remains between 3-4 ℃, humidity will have ventilation hole 90% in the cellar for storing things.

2.. with the breeding potato of select, through after the strict sterilization, carry out vernalization annual April, be placed in the sterile cellar for storing things, shut Jiao Men, between 6-10 ℃, humidity is under 90% condition, greatly about the 5-8 month in unglazed sharp temperature, bear the living potato of dream of gene mutation at eye, the living potato of dream is grown up when 10-30 restrains, after the potato piece takes off, be placed in the sterile cellar for storing things of another one, shut Jiao Men, with in late May, second plantation, September 20 gathered after 360 days in 280 days in dormancy in the environment of unglazed, low temperature, hypoxemia.

The living potato of dream is to grow out from the eye of potato, so its contained gene is identical with the gene of terrestrial stem, plant the potato piece appearance that and representing the color of terrestrial stem, terrestrial stem is purple, blue, black, and it just shows on the potato piece that is produced.Because its this specific character is that regeneration strain is the basic reason of chromosome and mitosis variation.

2. with after the potato breeding stripping and slicing of choosing, soaked 2 hours with 0.01% iodophor solution, aqueous vapor is dried, plant in late June, grow into September 10, with the potato terrestrial stem, after cutting in fine noon or afternoon, cut leaf, use 58% metalaxyl-mn-zn, 500 times of liquid of wetting powder soaked 20 minutes, after aqueous vapor is dried, buried in the ground.The degree of depth is 5 centimetres, line-spacing is 15 centimetres, and native humidity must be big, but can not become mud, and cover mulch film in the above, after 15 days, on each leaf bud base, grow a little potato of little shape greatly, after growing into 40 days, it is dug out take off little potato, be placed in the sterile cellar for storing things, in mid-May, second plantation, it absorbs the position difference of nutriment, only absorbs the nutriment of potato cane.So the potato shape difference that it produces, gene are also different.

More than two kinds of breeding techniques, outstanding and the seed breeding technique that grows directly from seeds, each grain of the potato piece that is produced all is improved seeds, with the seed crossbreeding of growing directly from seeds, the potato that brings out sometimes can not select a decent kind, all is eliminated.