CN101961059B - Puer tea extract, preparation method and application - Google Patents
Puer tea extract, preparation method and application Download PDFInfo
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Abstract
The invention relates to a Puer tea extract, a preparation method and application. The Puer tea extract contains effective ingredients of tea polyphenol, tea Polysaccharide, theabrownine, caffeine and the like the content of which are high, and can obviously lower blood sugar. The preparation method can effectively convert and dissolve out the effective ingredients. The process route can assure clarity after the cold water of a finished product is dissolved, lowers the late centrifugation workload, has low cost and stable and controllable quality and is industrial.
Description
Technical field
The present invention relates to a kind of tea product, relating in particular to is a kind of hypoglycemic Pu'er tea that has.
Background technology
In recent years; Because the variation of people's production, life and environmental condition, be that whole resource is damaged on the one hand, air, water quality receive pollution in various degree, residual in part vegetables, the fruit have an agricultural chemicals; Feed hormone in the part meat fails to decompose fully; Increased the weight of the operating pressure of human body toxin expelling organ pancreas greatly, often this causes insulin production quantity not sufficient in the body in the past; Be the significantly raising of social productiveness of labor on the other hand; For society has brought extremely abundant physical product; Alleviate simultaneously people's manual labor in a large number, reduced physical demands, accumulating heat in the body increases; Cause the insulin consumption to increase, the result of two aspect comprehensive functions is that the incidence of disease of type disease such as diabetes, fatty liver is significantly increasing.At present, diabetes become harm humans healthy be only second to cardiovascular and cerebrovascular disease, cancer, be to occupy tertiary disease.According to statistics, the incidence of disease of diabetes is 3～5% in the world, and the incidence of disease of China is that crowd's more than 3.21%, 50 years old the incidence of disease is 10%.
Diabetes spp is in endocrine system disease, is because hypoinsulinism in the body, causes the disorderly and disease that causes of sugar, protein and water-electrolyte metabolism, and it directly destroys heart and brain, kidney, eye and nerve fiber organ.Diabetes are divided type 1 diabetes (type1 diabetes) and diabetes B (type 2 diabetes) and gestational period diabetes (gestational diabetes).Wherein the type 1 diabetes pilosity is born in the teenager, and its insulin secretion lacks, and must rely on insulinize and earn a bare living.During diabetes B is more common in after 30 years old, the elderly, its secretion of insulin amount is not low even also higher, the cause of disease mainly is that body is to insulin insensitivity (being insulin resistance).Gestational period diabetes (gestational diabetes) are the insulin resistances that comes from cell, and the hormone (hormone) of pregnancy women secretion causes but its insulin resistance is.The gestational period, diabetes were usually in minute puerperium self-healing.
Insulin is hypoglycemic hormone only in the health of human pancreas β emiocytosis.Insulin resistance is meant that surrounding tissue is to the sensitiveness reduction of insulin in the body, and tissue is to insulin insensitivity, and peripheral tissues such as muscle, fat promote the effect of glucose uptake that opposing has taken place to insulin.Discover that insulin resistance is prevalent in the diabetes B, almost accounts for more than 90%.The diabetes B patient had just had chronic complicating diseases to take place before making a definite diagnosis.According to statistics, have the 50% new diabetes B patient who diagnoses to have one or more chronic complicating diseases, some patient just finds to suffer from diabetes because of complication
Use antidiabetic drug clinically now or insulin is treated; Though can effectively control blood sugar; But the toxic and side effect of long-term prescription and bad reaction also threaten patient's safety and health, but the price of these medicines is all than higher, and the patient is difficult to accept; Often give up halfway, cause more serious consequence.Have yet and adopt the Chinese medicine preparation hypoglycemic, as the saying goes " being three fens poison of medicine ", health is also produced certain influence, and because the Chinese medicine preparation flavour of a drug are very big, take difficulty, the patient often can adhere to and all that has been achieved is spoiled.Seek thus one safe and effective, and the preparation that the patient is taken voluntarily for a long time is too impatient to wait.
Pu'er tea is the distinctive local well-known tea in Yunnan.The place of production has a moderate climate, and rainfall is abundant, and cloud and mist curls up.Pu'er tea.Be that large leaf with the original producton location, Yunnan shines blue or green tea and reprocessing forms two series: directly reprocessing be that the life of finished product is general and reprocess form ripe general through artificial rapid-result fermentation back, divides two types of loose tea and compressed teas on the type system again; All also continue behind the finished product carrying out natural ageing process, have the fragrant unique quality of Chen Yue more.
Pu'er tea is the tea of only after fermentation type, and harmful material such as its theophylline, Tea Polyphenols is divided to have melted in long-term sweat, so moral character is gentle; Human body is not stimulated; Can also enhance metabolism, quicken clearing up and transforming of fat, toxin in the health, perplex urbanite's problems such as obesity, three fat height now; Pu'er tea can both play good mitigation, as toxin expelling, nourishing the stomach, anti-inflammatory, reduction cholesterol, the fat that disappears go to be bored with, cosmetic slimming.
Patent 200510010871.2 discloses a kind of Pu'er tea and application thereof, and the preparation process is following
A. take by weighing a certain amount of Pu'er tea, be crushed to 20 orders, the temperature that adds 5-10 times of volume is 80～90 ℃ a distilled water, adds a cover insulation and soaks filtration after 40 minutes;
B. to use the temperature of 3-5 times of volume be that 80～90 ℃ distilled water immersion filtered after 30 minutes to filter residue;
C. to use the temperature of 3 times of volumes again be that 80～90 ℃ distilled water immersion filtered after 30 minutes to filter residue;
D. the filtrating that merges three filtrations; Adding absolute ethyl alcohol to concentration of alcohol and be 50%～90% scope precipitates; Leave standstill after 24 hours and filter, the collecting precipitation thing carries out vacuum dehydrating at lower temperature; Vacuum and low temperature is 50～60 ℃, is dried to moisture and is about 5～11% and promptly gets Pu'er tea.Contain tea polysaccharide 15-45% in the Pu'er tea, theabrownin 50-70%, protein 4.7-14.0% etc.
Extract it is thus clear that this preparation technology is middle low temperature, then with extracting liquid filtering, the filtered fluid decompression concentrates; Spray-drying or drying under reduced pressure form, and these process routes are in order to reduce the difficulty of filtration, and low temperature extracts in basically all carrying out; Product active ingredient kind and yield are all lower; " mould " flavor of Pu'er tea fermented tea is arranged, and the unstability of filter operation efficient and effect is higher, can cause negative effect to indexs such as the cost of product, clarity.The inventor adopts new isolation technics in to the Pu'er tea research process, test out one group of new Pu'er tea and preparation method thereof; These Pu'er teas have the curative effect height, and purity is high, good absorbing; Steady quality, technology is simple, and is easy to operate; Composition is cheap, is fit to industrialization production.
Summary of the invention
In order to solve the problems of the technologies described above, the present invention provides a kind of determined curative effect, safe and reliable Pu'er tea.
Purpose of the present invention provides the composition and the preparation thereof of this extract.
Another object of the present invention provides this preparation method of extract.
A further object of the invention provides this extract and the preparation purposes in the treatment diabetes.
It is Tea Polyphenols, tea polysaccharide, theabrownin, caffeine that Pu'er tea of the present invention contains main active.
Its weight percentage is:
Tea Polyphenols 12-55%
Theabrownin 12-30%
Caffeine 6-11%
Tea polysaccharide 15-40%
Wherein the weight percentage sum of four kinds of active ingredients is less than 100%.
The active ingredient of preferred weight percentage composition:
Tea Polyphenols 20-50%
Theabrownin 15-25%
Caffeine 6-10%
Tea polysaccharide 17-30%
Wherein the weight percentage sum of four kinds of active ingredients is less than 100%.
More preferably weight percentage:
Tea Polyphenols 30-45%
Theabrownin 16-20%
Caffeine 6-8%
Tea polysaccharide 17-23%
Wherein the weight percentage sum of four kinds of active ingredients is less than 100%.
The surplus of said extracted thing is protein, theaflavin, congo red element or pectin etc.
Above-mentioned Pu'er tea is obtained by following preparation method:
Get Pu'er tea, add water and acutely seethe with excitement and decoct to extract 1-5 time (preferred 2-4 time), decocting time 0.5-5 hour (preferably 2-4 hour); Add 5-50 times of water of water general times (preferred 18-26 doubly); Extract concentrates: concentrate two times centrifugal, centrifugate concentrate condensed cream, condensed cream is drying to obtain extract.
Preferred above-mentioned extract 40-300 order filters (preferred 40-80 order); Filtrating is concentrated into tealeaves (weight): concentrate (volume)=1: 0.5-1: 10 (preferred 1: 2-1: 3); The concentrate secondary centrifuging; Secondary centrifuging liquid is concentrated into proportion 1.01-1.4 (preferred 1.13-1.20)/55-65 ℃), condensed cream is drying to obtain extract, and extract yield is 12-30%.
Described filtrating method for concentration includes but not limited to that decompression concentrates, normal pressure concentrates, rotating thin film concentrates, horizontal centrifugal thin-film concentrates, reverse osmosis concentration, film concentrate, single-action concentrates, economic benefits and social benefits concentrate, triple effect concentrates, spherical concentrate etc.; Preferred temperature≤70 ℃, decompression concentrates.Described drying means includes but not limited to microwave drying, vacuum belt type drying;, spray-drying, freeze drying, far-infrared ray drying, steam drying etc.; Preferred microwave drying, vacuum belt type drying or spray-drying, this drying means, heated time is short; Drying efficiency is high, can reduce the destruction of high temperature to active component.
Described secondary centrifuging is preferred, and concentrate is centrifugal with tripod pendulum type batch centrifugal for the first time, and centrifugate uses tube centrifuge centrifugal again.The two times centrifugal process sequence can not be put upside down, and concentrate first order tripod pendulum type batch centrifugal centrifuging process can reduce the centrifugal workload of secondary tubular type, removes a large amount of impurity, adopts the clarity after the 2nd grade of centrifuging process can ensure finished product cold water, hot water dissolving.
The preparation method of Pu'er tea of the present invention may further comprise the steps:
(1) extract: Pu'er tea, add water and acutely seethe with excitement to decoct and extract 1-5 time, decocting time 0.5-5 hour, add water general times 5-50 doubly, get extract;
(2) concentrate: extract 40-300 order filters, and filtrating is concentrated into tealeaves (weight): concentrate (volume)=1: 0.5-1: 10, get concentrate;
(3) condensed cream preparation: the concentrate secondary centrifuging, secondary centrifuging liquid is concentrated into proportion 1.01-1.4, gets condensed cream;
(4) extract: condensed cream is drying to obtain.
Above-mentioned described Pu'er tea is through behind the concentrate drying, and extract yield is for being 12-30%.
Preferably adding water inventory in the said step (1) is 18-26 times, decocts and extracts decocting time 2-4 hour 2-4 time.Preferred extract is crossed the 40-80 mesh sieve in the step (2), and filtrating is concentrated into tealeaves: concentrate=1: 2-1: 3, get concentrate.Preferred concentrate is earlier once centrifugal with tripod pendulum type batch centrifugal in the step (3), and centrifugate is used the tube centrifuge secondary centrifuging, and secondary centrifuging liquid is concentrated into proportion 1.13-1.20.
Concrete steps are: Pu'er tea adds water and acutely seethes with excitement and decoct to extract decocting time 2-4 hour 2-4 time; Add water general times 18-26 doubly, extract 40-80 order filters, and filtrating temperature≤70 ℃ are evaporated to tealeaves (weight): concentrate (volume)=1: 2-1: 3; The concentrate tripod pendulum type batch centrifugal is centrifugal; One time centrifugate is centrifugal with tube centrifuge, and secondary centrifuging liquid ℃ is evaporated to proportion 1.13-1.20/55-65 ℃ in temperature≤70, and condensed cream is dry to get final product.This method gained Pu'er tea stable yield, yield is 23-27%.
The present invention adopts violent boiling to help the conversion and the stripping of active ingredient; This process route can ensure the clarity behind the cold-water solution of finished product; This process route has reduced centrifugal workload of later stage, but the lower industrialization of this process route cost, stable and controllable for quality.
The application of Pu'er tea of the present invention in food industry, pharmaceuticals industry, health care industry or natural prodcuts are produced.
The application of Pu'er tea of the present invention in pharmaceuticals industry:
Pu'er tea of the present invention can be processed and contain the pharmaceutical composition that Pu'er tea is an active component.
Composition of the present invention can also be processed pharmaceutically acceptable preparation, and wherein Pu'er tea shared percentage by weight in pharmaceutical preparation is 0.1～99.9%, and all the other are the medicine acceptable carrier.
Pharmaceutical composition of the present invention exists with unit dosage form, and said unit dosage form is meant the unit of preparation, as every of tablet, every of capsule etc.,
Preparation of the present invention includes but not limited to tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, sucks agent, granule, electuary, pill, powder, paste, sublimed preparation, supensoid agent, pulvis, solution, injection, suppository, ointment, emplastrum, creme, spray, drops, patch.
The pharmaceutical composition of invention, the preparation of its oral administration can contain excipient commonly used, such as adhesive, filler, diluent, tablet agent, lubricant, disintegrant, colouring agent, flavor enhancement and wetting agent, can carry out dressing to tablet in case of necessity.
The filler that is suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrant comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example dolomol.The acceptable wetting agent of appropriate drug comprises lauryl sodium sulfate.Can fill through mixing, the method that compressing tablet etc. are commonly used prepares solid oral composition.Mix repeatedly active material is distributed in those compositions of a large amount of fillers of whole use.The form of oral liquid for example can be water-based or oily suspensions, solution, emulsion, syrup or elixir, perhaps can be a kind of available water before use or other suitable composite dry products of carrier.This liquid preparation can contain conventional additive; Such as suspending agent; For example sorbierite, syrup, methylcellulose, gelatin, hydroxyethylcellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat; Emulsifying agent, for example lecithin, anhydro sorbitol monooleate or Arabic gum; Non-aqueous carrier (they can comprise edible oil), for example apricot kernel oil, fractionated coconut oil, such as oily ester, propane diols or the ethanol of the ester of glycerine; Anticorrisive agent, for example para hydroxybenzene methyl esters or propylparaben or sorbic acid, and if desired, can contain conventional flavouring agent or colouring agent.
For injection, the liquid unit dosage forms of preparation contains active material of the present invention and sterile carrier.According to carrier and concentration, can this compound be suspended or dissolving.The preparation of solution is normally through being dissolved in active material in a kind of carrier filter-sterilized before it is packed into a kind of suitable bottle or ampoule, sealing then.For example a kind of local anesthetic of auxiliary material, anticorrisive agent and buffer also can be dissolved in this carrier.In order to improve its stability, can be after the bottle of packing into that this composition is freezing, and under vacuum, water is removed.
Pharmaceutical composition of the present invention; When being prepared into medicament, optionally add suitable medicine acceptable carrier; Said medicine acceptable carrier is selected from: sweet mellow wine, sorbierite, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDTA disodium, EDTA calcium sodium, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivative thereof, alginates, gelatin, polyvinylpyrrolidone, glycerine, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, polyethylene glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, kaolin, talcum powder, calcium stearate, dolomol etc.
Pharmaceutical composition of the present invention is confirmed usage and dosage according to patient's situation in use, but obeys every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet, every dose of 1mg-1000mg.
Pu'er tea of the present invention can also be in the application on food and the health food.This extract can be processed tea-drinking article, tea in bag, Pu'er tea paste, tea beverage etc.
Beneficial effect of the present invention describes through following Test Example.
The influence of a pair of normal mouse blood sugar of Test Example
1, test material
1.1 experimental animal: 40 of KKAy mouse, female, age in 7-8 week, rank SPF level, body weight 33.5 ± 1.9g is provided by Chinese Academy of Medical Sciences animal used as test.
1.2 reagent and medicine
Pu'er tea: the embodiment of the invention 1, the dark brown powder, water-soluble, solubility is good, lucifuge.
Positive drug: Rosiglitazone Maleate sheet
1.3 laboratory apparatus:
BIOSEN5030 type blood sugar/lactic acid analyzer
Hitachi's 7080 full automatic biochemical apparatus
2.1 experimental technique
2.1 fasting blood-glucose assay method:
All animals all in 9 beginnings of commercial affairs fasting, are got blood behind the fasting 4h, get preceding each the treated animal gastric infusion of 1h of blood, cut tail when getting blood and get tip blood 10ul, with BIOSEN5030 type blood sugar/lactose analysis-e/or determining fasting blood-glucose.
2.2 divide into groups and administration
Animal adaptability is measured fasting blood-glucose according to 2.1 methods after feeding a week, divides into groups according to the fasting blood sugar stratified random of measuring.40 KKAy divide into groups according to the fasting blood sugar stratified random, are divided into 4 groups, model group, positive controls, experimental group.
Crowd's operational version, it is 3g/ people/day that the crowd intends using dosage, i.e. 0.05g/kg/d, the use approach brews to be drunk.
Positive controls, administration every day 1 time, dosage 1.33mg/kg/ day, oral administration gavage administration.
Test group: the animal test sample amount of drinking reaches the dosage requirement of 1.5g/kgBW, and replaces drinking water every day with the Pu'er tea of this concentration, gives for 4 weeks continuously.
2.3 test procedure
2.3.1 the weight of animals and feed situation, ight soil situation of change: observe animal spirit, activity, hair color, food-intake, amount of drinking water and changes of weight
2.3.2 fasting blood-glucose is measured: the set time is measured fasting blood-glucose according to 2.1 methods weekly before the administration and after the administration.
2.3.3 sugar tolerance experiment
Tested the 21st day, and after animal is measured fasting blood-glucose according to 2.1 methods, irritated clothes glucose 2.5g/kg, respectively at 0.5h, blood glucose value, area under the calculated curve (AUC) AUC=0.5 * fasting blood-glucose+0.5h blood sugar+1.5 * 1h blood sugar+2h blood sugar are measured in 1h, 2h blood sampling.
2.3.4 serum electrolyte is measured
When testing the 22nd day, pluck eyeball behind the animal fasting 16h and get blood, separation of serum is surveyed mensuration serum K ion, Na ion, Ca ion concentration with the XD685 blomelicalbloodgasandelectrolrteanalyzers.
2.3.5 triglycerides, T-CHOL are measured;
When testing the 29th day, pluck eyeball behind the animal fasting 16h and get blood, separation of serum is measured serum triglyceride, total cholesterol level with full automatic biochemical apparatus.
3, result of the test
3.1 the weight of animals, food-intake changes result of the test
The experimental session experimental animal is in good condition, and food-intake, amount of drinking water are stablized.
Experimental group was freely drunk for two weeks according to dosage 1.5g/kg/ day continuously, and food-intake and body weight all change not quite, each treated animal body weight, and the food-intake result of variations is seen table 1, table 2.
Table 1 the weight of animals situation of change (g)
Annotate: compare with model
*P＜0.05.
Each treated animal food-intake situation of change (g) of table 2
3.2 fasting blood-glucose determination test result
The result sees table 3, and after 7 days, fasting blood-glucose all is starkly lower than model group (p＜0.01) to the experimental group experimental animal in administration, positive controls, and after 14 days, fasting blood-glucose all is starkly lower than model group (p＜0.01, p＜0.01) to the experimental group animal in administration.It is thus clear that Pu'er tea has obvious hypoglycemic activity.
Table 4 Pu'er tea is to the influence of fasting blood-glucose (nmol/l)
Annotate: compare with model
*P＜0.01,
* *P＜0.01.
3.3 carbohydrate tolerance test result:
Visible by table 4; In the test of anti-sugar amount; The blood glucose value of each time point of model group and AUC value all are higher than control group, and the blood glucose value and the AUC of each time point of positive drug group all are lower than model group, and experimental group is in 0h, 0.5h, 1h and AUC value and model group there were significant differences p＜0.01; P＜0.001., visible Pu'er tea can suppress the rising of blood glucose value after the oral sucrose of mouse.
Table 4 Pu'er tea is to the influence of sugar tolerance
Annotate: compare with model group
*P＜0.01,
* *P＜0.001.
3.4 serum electrolyte is measured the result
Visible by table 5, the positive drug group diabetic mice K that can obviously raise
+,Na
+,Cl
-Concentration (p＜0.001), the experimental group Na that can obviously raise
+,Cl
-Concentration (p＜0.01, p＜0.001)
Table 5 Pu'er tea is to the influence of serum electrolyte
| Divide into groups | n | K + | Na + | Cl - | Ca 2+ |
| The KKAy model group | 10 | 6.07±0.60 | 151.38±2.77 | 113.90±1.77 | 1.04±0.11 |
| Positive controls | 10 | 7.02±0.57** | 156.19±2.07*** | 118.48±1.89*** | 1.10±0.06 |
| Test group | 10 | 6.36±0.34 | 155.07±2.14** | 117.18±1.28*** | 1.06±0.08 |
3.6 triglycerides, T-CHOL are measured the result
Visible by table 6, administration in the time of 28 days triglycerides (TG), T-CHOL (CHOL) measure among the result, each experimental group does not have influence to total cholesterol level.The positive drug group, the Pu'er tea high dose group, the low dose group content of triglyceride has been compared the reduction effect with model group, and significant difference (p＜0.05) is arranged.
Table 6 Pu'er tea triglycerides, T-CHOL result
Annotate: compare with model group
*P＜0.05
Compare with normal group
△ △P＜0.01,
△ △ △P＜0.001
4, conclusion (of pressure testing)
To each treated animal food-intake, the continuous observation of amount of drinking water and body weight gain finds that positive drug and experimental group are to the animal food-intake through experimental session, and the body weight influence is little, and is not only smaller than model group on the numerical value, and not statistically significant.From fasting blood-glucose determination test result, Pu'er tea all has the effect of tangible reduction fasting blood-glucose.The beta-oxybutyria acid poisoning is the severe complication of diabetes; Such patient is because relative or absolute shortage of insulin; Tissue utilizes the glucose ability drop; Catabolism of fat and gluconeogenesis strengthen, and liver generation ketoboidies increases and causes hyperglycaemia and ketosis, thereby brings out a series of water, electrolyte and disturbance of acid-base balance.Can find out that through the mensuration of serum electrolyte Pu'er tea has certain regulating action to the diabetes electrolyte disturbance.
In the investigation experiment of effect for reducing fat, Pu'er tea shows can reduce content of triglyceride.
Interior all extracts of claims protection domain of the present invention and the extract in the specific embodiment, preparation method have above-mentioned effect and effect thereof.
Pu'er tea active constituent content of the present invention is high; Can significantly reduce blood sugar; This preparation method can effectively transform active ingredient and stripping; This process route can ensure the clarity behind the cold-water solution of finished product, and this process route has reduced centrifugal workload of later stage, but the lower industrialization of this process route cost, stable and controllable for quality.
The specific embodiment
With specific embodiment the present invention is described below, embodiment is for the ease of understanding the present invention, rather than limits claim of the present invention and core content by any way.
Embodiment 1,
Pu'er tea adds water and acutely seethes with excitement and decoct to extract decocting time 4 hours 3 times; Add 26 times of water general times, 10 times for the first time, 8 times for the second time; 8 times for the third time, extract 80 orders filter, and filtrating temperature≤70 ℃ are evaporated to tealeaves (weight): concentrate (volume)=1: 2-1: 3; The concentrate tripod pendulum type batch centrifugal is centrifugal; One time centrifugate is used the tube centrifuge secondary centrifuging, and secondary centrifuging liquid temp≤70 ℃ are evaporated to proportion 1.13-1.15/55-65 ℃, and condensed cream microwave drying promptly gets Pu'er tea.Wherein extract yield is 26%, through assay, contains Tea Polyphenols 49.95% in the Pu'er tea, theabrownin 24.87%, caffeine 9.80%, tea polysaccharide 15.21%, theaflavin 0.17%.
Embodiment 2
Pu'er tea adds water and acutely seethes with excitement and decoct to extract decocting time 0.5 hour 1 time; Add 5 times of water general times, extract 60 orders filter, and filtrating temperature≤70 ℃ are evaporated to tealeaves (weight): concentrate (volume)=1: 0.5; The concentrate tripod pendulum type batch centrifugal is centrifugal, and one time the centrifugate secondary is centrifugal with tube centrifuge, and secondary centrifuging liquid temp≤70 ℃ are evaporated to proportion 1.01/55-℃; The condensed cream spray-drying promptly gets Pu'er tea, and wherein extract yield is 12%.Through assay, contain Tea Polyphenols 54.59% in the Pu'er tea, impurity 3.33% such as theabrownin 12.05%, caffeine 5.5%, tea polysaccharide 24.48%, theaflavin 0.05%, protein pectin.
Embodiment 3
Pu'er tea adds water and acutely seethes with excitement and decoct to extract decocting time 5 hours 5 times; Add 30 times of water general times, add 6 times of water yields at every turn, extract 300 orders filter; Filtrating temperature≤70 ℃ are evaporated to tealeaves (weight): concentrate (volume)=1: 10; The concentrate tripod pendulum type batch centrifugal is centrifugal, and one time the centrifugate secondary is centrifugal with tube centrifuge, and secondary centrifuging liquid temp≤70 ℃ are evaporated to proportion 1.4/65-℃; The condensed cream vacuum belt type drying promptly gets Pu'er tea, and wherein extract yield is 28%.Through assay, contain Tea Polyphenols 35.46% in the Pu'er tea, theabrownin 16.51%, caffeine 6.79%, tea polysaccharide 18%, theaflavin 0.05%., protein and pectin impurity etc. 23.19%.
Embodiment 4
Pu'er tea adds water and acutely seethes with excitement and decoct to extract decocting time 5 hours 2 times; Add 26 times of water general times, extract 80 orders filter, and filtrating temperature≤70 ℃ are evaporated to tealeaves (weight): concentrate (volume)=1: 2-1: 3; The concentrate tripod pendulum type batch centrifugal is centrifugal; One time the centrifugate secondary is centrifugal with tube centrifuge, and secondary centrifuging liquid temp≤70 ℃ are evaporated to 1.4/65 ℃ of proportion, and condensed cream microwave drying promptly gets Pu'er tea.Wherein extract yield is 25%, through assay, contains Tea Polyphenols 20.95% in the Pu'er tea, theabrownin 16.51%, caffeine 7.70%, tea polysaccharide 34.73%, congo red plain 1.91%., impurity 18.2% such as protein pectin.
Embodiment 5
Pu'er tea adds water and acutely seethes with excitement and decoct to extract decocting time 5 hours 2 times; Add 50 times of water general times, extract 40 orders filter, and filtrating temperature≤70 ℃ are evaporated to tealeaves (weight): concentrate (volume)=1: 2-1: 3; The concentrate tripod pendulum type batch centrifugal is centrifugal; One time the centrifugate secondary is centrifugal with tube centrifuge, and secondary centrifuging liquid temp≤70 ℃ are evaporated to 1.2/65 ℃ of proportion, and the condensed cream spray-drying promptly gets Pu'er tea.Wherein extract yield is 27%, through assay, contains Tea Polyphenols 20.95% in the Pu'er tea, theabrownin 16.51%, caffeine 7.70%, tea polysaccharide 40.23%, congo red plain 1.91%., impurity 12.7% such as protein pectin.
Embodiment 6 tea polysaccharide content assaying methods
A.1 instrument and reagent
A.1.1 instrument
Ultraviolet-visible spectrophotometer; Miniature whirlpool mixed instrument; Ten thousand/balance; Ultrasound Instrument.
A.1.2 test sample and reagent
Test sample: instant Pu'er tea
Reagent: re-distilled phenol; Sulfuric acid.
Reference substance: D-DEXTROSE ANHYDROUS, Nat'l Pharmaceutical & Biological Products Control Institute's (supplying assay to use).
A.2 test
A.2.1 the preparation of need testing solution
The about 0.2g of these article of getting extract powder (being accurate to 0.001g) puts in the 10ml measuring bottle, and it is an amount of to add water, and dissolving in ultrasonic 20 minutes is cooled off and added water to scale, shakes up.Precision is measured 1ml, puts in the 100ml measuring bottle, and thin up shakes up to scale, as need testing solution.
A.2.2 the preparation of reference substance solution
It is an amount of to get D-DEXTROSE ANHYDROUS reference substance, accurate claims fixed (being accurate to 0.001g), is dissolved in water and dilutes and process the solution that contains 0.2mg among every 1ml, as reference substance solution.
A.2.3 the drafting of calibration curve
Accurate respectively absorption reference substance solution 0.0,0.1,0.2,0.3,0.4,0.5ml put respectively in the 10ml tool plug test tube; Add water to 1.0ml respectively, each is accurate to add 5% phenol solution and (takes by weighing the 2.5g re-distilled phenol, be dissolved in water and be diluted to 50ml; Shake up, promptly get.) 1.0ml; The jolting mixing adds 5.0ml sulfuric acid, with the rapid jolting mixing of miniature whirlpool mixed instrument; Room temperature held 30 minutes; With the 1st pipe is blank, according to UV-VIS spectrophotometry (appendix VB of Chinese Pharmacopoeia version in 2005), measures absorbance (in 1 hour, measure and finish) in the 487nm wavelength.Make calibration curve with absorbance (Y) and quality (X), regression equation is:
Y＝a·X+b
In the formula:
Y is the absorbance of reference substance solution;
X is the quality (mg) of D-DEXTROSE ANHYDROUS;
A, b are constant.
A.2.4 the mensuration of need testing solution
The accurate 1ml need testing solution of drawing under the drafting item according to calibration curve, from " the phenol solution 1.0ml of accurate adding 5% ", with the method operation, is measured absorbance respectively at the 487nm place.
A.2.5 data
Be calculated as follows content:
In the formula:
C is the tea polysaccharide percentage composition in the sample;
A is the absorbance of sample;
W is sample weighting amount (g);
A, b are constant.
Embodiment 7 Content Determination Method of Green Tea Polyphenol
Principle: polyphenols can generate the hyacinthine complex compound with ferrous ion.With its content of spectrophotometry.Though various catechins be the colourity difference, the catechin compositing range in the Tea Polyphenols is roughly the same, and is little to the influence of absorbance in this scope, so get final product with a calibration curve.This calibration curve is consistent with the calibration curve of (one) Epigallo-catechin gallate (EGCG), but because of (one) Epigallo-catechin gallate (EGCG) is difficult to obtain, so use progallin A.Because of the absorbance of 10mg progallin A equates with the absorbance of 15mg () Epigallo-catechin gallate (EGCG), so regulation multiply by 1.5 conversion coefficients as Tea Polyphenols from the amount that the calibration curve of progallin A obtains.
Annotate: " (one) table "---expression " left-handed cis ".
5.2.2 reagent and solution
5.2.2.1 tartaric acid ferrous solution
Take by weighing ferrous sulfate (GB 664) 1.0g, sodium potassium tartrate tetrahydrate (GB 1288) 5.0g is dissolved in water and is settled to IL, and this liquid can be stablized 10 days.
5.2.2.2pH7.5 phosphate buffer
The disodium phosphate soln of a liquid 1/15mol/L: take by weighing sodium hydrogen phosphate (GB 1263) 23.877g, be dissolved in water and be diluted to IL.
The potassium dihydrogen phosphate of b liquid 1/15mol/L: take by weighing potassium dihydrogen phosphate (GB1274) 9.078g, be dissolved in water to IL through 110 ℃ of oven dry 2h.
Get a liquid 85mL and b liquid 15mL mixing, promptly get the slow juice of pH7.5.
5.2.2.3 the preparation of standard liquid
Accurately take by weighing progallin A (100 ℃ oven dry dry th) 250mg, be dissolved in the 100mL water, draw mother liquor 2 respectively as mother liquor; 4,6,8; 10mL is mixed with constant volume not and contains progallin A 50,100 among the 100mL in the 10mL volumetric flask; 150,200, the standard liquid of five kinds of variable concentrations of 250mg.
5.2.3 instrument
Spectrophotometer.
5.2.4 determination step
5.2.4.1 the making of standard working curve
The progallin A standard liquid ImL and the tartaric acid ferron 5mL of the variable concentrations of accurately drawing place the volumetric flask of a series of 25mL, with the buffer solution constant volume of pH7.5.Water replaces progallin A as contrast, and the cuvette of railway carriage or compartment 1cm is measured absorbance at the 540nm place.The absorbance of being surveyed is depicted as standard working curve with corresponding progallin A concentration.
5.2.4.2 the preparation of test liquid and mensuration
Preparation: accurately take by weighing tea extraction 200mg, place the 100mL beaker, add the boiling water dissolving more than 20～30mL90 ℃, cooling moves in the 100mL volumetric flask, and constant volume, filtration discard the about 20mL of initial filtrating, and last filtrating is test liquid.
Measure: accurately draw test liquid ImL, put in the 25mL volumetric flask, tartarize ferrous solution 5ml, abundant mixing is with the phosphate buffer constant volume of pH7.5.Make reference with reagent blank liquid, measure absorbance in the 540nm place.
5.2.4.3 the result calculates
According to standard working curve, obtain the corresponding content of the progallin A that is equivalent to the sample absorbance, obtain polyphenol content by formula (1):
Tea Polyphenols (%)=F * 1.5 * 100/m * (1-G)
In the formula: the absorbance that m-sample mass G-sample moisture E-records according to sample, from the corresponding content of progallin A that calibration curve checks in, mg/100ml
1.5-the conversion coefficient of Tea Polyphenols.
Embodiment 8 caffeine component content assay methods
(1), chromatographic condition: flowing phase (methyl alcohol: water=30: 70), detect wavelength 273nm, sample size (reference substance 10ul, test sample 5ul), column temperature (25 ℃), flow velocity (1ml/min)
(2), reference substance solution preparation: get the about 10mg of caffeine reference substance, the accurate title, decide, and puts in the 100ml volumetric flask, adds methyl alcohol 20ml and make dissolving, adds water and be settled to scale, filters, and promptly gets.
(3), need testing solution preparation: accurate claim decide extract be 0.5g in the 100ml conical flask, accurately add purified water 100ml, weigh, boiling water bath 1h weighs, and adds purified water and supplies weight, filtration promptly gets.
Embodiment 8 theaflavin, congo red element and theabrownin content assaying method
Principle: theaflavin, congo red element and theabrownin all are dissolved in hot water; Be present in the millet paste; Can from millet paste, come out the theaflavin extract and separate with ethyl acetate; But have part congo red plain (SI type congo red is plain) also to be suggested, this part congo red element can utilize it to be dissolved in sodium bicarbonate solution and remove further the separation thereupon, and SII type congo red element is stayed water layer.Theabrownin is insoluble to n-butanol, millet paste with extracting n-butyl alcohol after, theaflavin and congo red element all change and dissolve in n-butanol, theabrownin is stayed water layer.After each component separating, available spectrophotometric carries out colorimetric estimation like this.
Capital equipment and reagent
1, separatory funnel, spectrophotometer, water-bath, suction pipe, conical flask, 25ml volumetric flask etc.
2, ethyl acetate, n-butanol, 95% ethanol
3, after 2.5% sodium acid carbonate, 2.5g sodium acid carbonate are dissolved in water, be settled to 100ml.
When 4, saturated oxalic acid solution, 20 ℃ of temperature, solubilized 10.2g oxalic acid in the 100ml water can be according to the different preparation of temperature saturated solutions.
Experimental procedure
1, test liquid preparation: accurately take by weighing the 50-2000mg tea extraction, add boiling water 125ml, shake up back lixiviate 10 minutes in boiling water bath; Shake bottle in the lixiviate once; After lixiviate finished, taking-up shook up, and filtered in the conical flask of drying (residue does not need the water flushing) with cotton while hot; Filtrating can extract and spectrophotometry after soaking and in cold water, being chilled to room temperature.
2, extraction: shake up test liquid, draw 25ml and be placed in the 60ml separatory funnel, add the 25ml ethyl acetate; Speed jolting 5 minutes with secondary each second roughly; Static layering is emitted water layer respectively and is poured the ethyl acetate layer into (after the layering, middle opacifying layer discards) for use in the tool plug triangular flask.
Absorption ethyl acetate layer solution 2ml is placed in the 25ml volumetric flask, adds 95% ethanol and is diluted to 25ml, shakes up to be solution A.Draw ethyl acetate layer solution 10ml and be placed in the 30ml separatory funnel, add 2.5%NaHCO3 aqueous solution 10ml, 30 seconds of jolting.Behind the standing demix, emit NaHCO3 water layer solution immediately and discard, pour ethyl acetate layer solution in the tool plug test tube into carefully, get this ethyl acetate layer solution 4ml, be placed in the 25ml volumetric flask, add 95% ethanol and be diluted to 25ml, shake up and be solution C.
Draw the water layer solution 2ml that tells with ethyl acetate extraction for the first time, be placed in the 25ml volumetric flask, add 2ml saturated oxalic acid solution and 6ml distilled water, and be diluted to 25ml, shake up the back solution D with 95% ethanol.
Draw millet paste test liquid 10ml, be placed in the 30ml separatory funnel, add the 10ml n-butanol, jolting 3 minutes, leave standstill slowly layering after, emit following water layer.Draw this water layer solution 2ml, be placed in the 25ml volumetric flask, add 2ml saturated oxalic acid and 6ml distilled water, and be diluted to 25ml, shake up and be solution B with 95% ethanol.
3, colorimetric estimation
Select the 380nm wavelength, use the 1cm cuvette, make blank, measure the optical density E of A, B, C, D solution respectively with 95% ethanol.Calculate content by following empirical equation.
Embodiment 10
Get Pu'er tea 450g and different Fructus Hordei Germinatus 670g, glucose 200g, strawberry fruit powder 40g, strawberry essence 10g, PVPP 10g, dolomol 10g; Above raw material fully mix process softwood with an amount of ethanolic solution after, granulate 60 ℃ of forced air dryings; Make 1000; Whole grain, compacting promptly gets oral disnitegration tablet in flakes.
Embodiment 11
Get Pu'er tea 25%, xylitol 48%, maltose 6%, 14% cyclodextrin, 7% calcium lactate prepares the Pu'er tea soft capsule according to the soft capsule common process.
Embodiment 12
Pu'er tea 5.0g and 10g xylitol, the 0.8g oligoisomaltose, the 10g antierythrite, 0.05 Aspartame, all the other are the city Pu'er tea oral liquid of water.
Embodiment 13
Get Pu'er tea 99.9%, add therein after 0.1% dextrin mixes, granulate, be drying to obtain Pu'er tea granule agent or electuary.
Embodiment 14
Get Pu'er tea 58g, lotus leaf 29g, Momordica grosvenori 13g mix and process composition, cross 16 mesh sieves, 22 mesh sieves respectively, get the mid portion packing.Add external packing, promptly get tea in bag.
Embodiment 15
Get Pu'er tea 58g, mulberry leaf 28g, fruit of Chinese wolfberry 13g mix and process composition, cross 16 mesh sieves, 22 mesh sieves respectively, get the mid portion packing.Add external packing, promptly get tea in bag.
Claims (12)
1. Pu'er tea, it comprises the active ingredient of following weight percent:
Tea Polyphenols 20-50%
Theabrownin 15-25%
Caffeine 6-10%
Tea polysaccharide 17-30%
Wherein the weight percentage sum of four kinds of active ingredients is less than 100%.
2. Pu'er tea as claimed in claim 1, it comprises the active ingredient of following weight percent:
Tea Polyphenols 30-45%
Theabrownin 16-20%
Caffeine 6-8%
Tea polysaccharide 17-23%
Wherein the weight percentage sum of four kinds of active ingredients is less than 100%.
3. claim 1 or 2 described Pu'er teas; It is characterized in that: obtain: get Pu'er tea, add water and acutely seethe with excitement and decoct to extract decocting time 0.5-5 hour 1-5 time by following preparation method; Add water general times 5-50 doubly; Extract concentrates: concentrate two times centrifugal, centrifugate concentrate condensed cream, condensed cream is drying to obtain extract.
4. a kind of Pu'er tea as claimed in claim 3 is characterized in that: described extract filters with the 40-300 mesh sieve before concentrating.
5. a kind of Pu'er tea as claimed in claim 3 is characterized in that: described extract is to be obtained by following preparation method: Pu'er tea adds water and acutely seethes with excitement and decoct to extract 1-5 time; Total decocting time 0.5-5 hour, add water general times 5-50 doubly, extract 40-300 order filters; Filtrating is concentrated into tealeaves: concentrate=1: 0.5～1: 10; The concentrate secondary centrifuging, secondary centrifuging liquid is concentrated into proportion 1.01-1.4, and condensed cream is drying to obtain extract.
6. any described Pu'er tea of claim 1～5 can be made into pharmaceutically acceptable composition.
7. composition as claimed in claim 6 is processed pharmaceutically acceptable preparation, and wherein Pu'er tea is as active constituents of medicine, and shared percentage by weight is 0.1～99.9% in preparation.
8. like the preparation method of any Pu'er tea of claim 1～5, it is characterized in that: may further comprise the steps: extract (1): Pu'er tea, and add water and acutely seethe with excitement to decoct and extract 1-5 time, total decocting time 0.5-5 hour, add water general times 5-50 doubly, get extract; (2) concentrate: extract 60-300 order filters, and filtrating is concentrated into tealeaves: concentrate=1: 0.5-1: 10, get concentrate; (3) condensed cream preparation: the concentrate secondary centrifuging, secondary centrifuging liquid is concentrated into proportion 1.01-1.4, gets condensed cream; (4) extract: condensed cream is drying to obtain.
9. like the preparation method of the said Pu'er tea of claim 8, it is characterized in that: may further comprise the steps: extract (1): Pu'er tea, and add water and acutely seethe with excitement to decoct and extract 2-4 time, total decocting time 2-4 hour, add water general times 18-26 doubly, get extract; (2) concentrate: extract 80 orders filter, and filtrating is concentrated into tealeaves: concentrate=1:: 2～1: 3, get concentrate; (3) condensed cream preparation: described concentrate is earlier once centrifugal with tripod pendulum type batch centrifugal, and centrifugate is used the tube centrifuge secondary centrifuging, and secondary centrifuging liquid is concentrated into proportion 1.13-1.20; (4) extract: condensed cream is drying to obtain, and extract yield is 12-30%.
10. the application of any described Pu'er tea of claim 1～5 in food industry, pharmaceuticals industry, health care industry or natural prodcuts are produced.
11. the application of any described Pu'er tea of claim 1～5 in preparation treatment diabetes medicament.
12. the application of the said Pu'er tea composition of claim 6 in preparation treatment diabetes medicament.
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| CN109662171A (en) * | 2018-12-27 | 2019-04-23 | 任秀江 | The preparation method and application of black tea beverage containing yellow fast cry of certain animals oxidase active inhibitor |
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