CN104095997B - A kind of application of Pu'er tea in the drug or food of preparation protection mucosal lesion - Google Patents

Abstract

The present invention relates to a kind of application of Pu'er tea in the drug or health food of preparation treatment mucosal lesion stomach trouble, wherein the mucosal lesion stomach trouble includes: gastritis, gastric ulcer, gastrorrhagia, erosion, hyperemia, oedema etc..Pu'er tea of the present invention is prepared according to the following steps: step 1, puerh tea leaves add water to cook, and are concentrated into certain weight proportion；Step 2, concentrate is centrifuged, centrifugate concentration, it is dry to get.

Description

A kind of Pu'er tea is in the drug or food of preparation protection mucosal lesion Using

Technical field

The present invention relates to a kind of Pu'er teas in the drug or food of preparation treatment mucosal lesion stomach trouble Using belonging to pharmacy and field of health care food.

Background technique

Pu'er tea the source area mainly Simao Diqu in Yunnan and Xishuangbanna.It is Sichuan, wide with the popularization of large leaf East, Guangxi also become the province of Pu'er tea production.With the development of economy, the enhancing of people's health care consciousness, Pu'er tea is because of its taste Taste mellow time sweet, and the unique fine quality of Chen Xiang and its concern and attention that the whole society is played to the special health-care efficacy of human body produce The deep favor by consumer of product.

Pu'er tea is warm-natured and storage tolerance, suitable for cooking use or bubble drink.The many historical book of Ancient Times in China have much about Pu'er tea function The record of effect: Zhao Xuemin's supplementary Amplifications of the Compendium of Materia Medica is recorded, general black as painted, and is sobered up first, is eliminated indigestion and phlegm, clearing stomach is promoted the production of body fluid, and power is outstanding It is big；Cloud, Pu'er tea paste can control all kinds of diseases and ailments, if tripe catches cold, be dissipated with ginger decoction again in " woody part ", and perspiration can be cured, and the broken larynx of mouth is dry, by Hot pain is chewed with five points and makes a slip of the tongue night i.e. more；Chen Zonghai writes, and " Pu'er, which is helped digest, disperses cold, there is solution for " interview of the Simao Room " record Toxic action." clear Song Shixiong " occupying diet spectrum with breath " cloud " Pu'er production person, highly seasoned kind wind phlegm of spitting disappear meat, all dirty string-shaped mass in the abdomen abdominal pain of heat, The diseases such as cholera dysentery are from the beginning of drink is often cured ".It can be seen that forefathers think Pu'er tea health-care experience, Pu'er tea, which has to help digestion, to be removed Poison, qi-regulating debulking, clearing heat and eliminating phlegm, wind dispelling are sobered up, and it is antibacterial and other effects to control dysentery.

Current research suggests that Pu'er tea has the health-care efficacy of several respects:

1, lower blood-fat and reduce weight: the metabolism of Pu'er tea and fat is in close relations, and Pu'er tea is generated by unique fermentation process New chemical substance, wherein lipase of some containing lipase, can generate decomposition, thus Pu'er tea to fat There is the effect of weight-reducing.

2, decompression, anti arteriosclerosis.

3, anti-cancer, anticancer: scientist is compared by a large amount of crowd, it was demonstrated that the cancer morbidity for the crowd that drinks tea is lower.And Pu'er tea contains there are many anticancer trace element abundant, and the effect of Pu'er tea killing cancer cell is strong.

4, be good for protecting teeth: physiologically active ingredient there are many containing in Pu'er tea has the function of sterilizing, therefore can remove Oral peculiar smell takes care of one's teeth.

5, stomach, nourishing the stomach are protected: under suitable concentration, drinking gentle Pu'er tea and stimulation is not generated to stomach, sticky, Sweet cunning, mellow Pu'er tea enter the surface layer of the film attachment stomach of human body stomach formation, and beneficial protective layer is generated to stomach, long-term It can play the role of protecting stomach, nourishing the stomach after drinking Pu'er tea.This is to advocate the consumer's appellation Pu'er for drinking Pu'er tea both at home and abroad The main reason for tea is " cosmetic tea ", " lengthen one's life tea ".

6, anti-aging: having shown that through research, and lipid peroxidation process is one of mechanism of human senility in human body.In Pu'er tea Vitamin C, vitamin E, tea polyphenols, amino acid and microelement for containing etc. have antioxidation, delaying senility course, because This Pu'er tea is referred to as " lengthen one's life tea ".

What 7, radiation protection: shown to drink 2% Pu'er with the result of study that Pu'er tea carries out according to Guangdong Zhongshan University state's fence etc. Tea can release the injury caused by co-60 radiation.

8, sober up: supplementary Amplifications of the Compendium of Materia Medica carries: " general tea most controls oil and covers pericardium, and postcibal diarrhea sobers up first." medical evidence: tea Tea polyphenols in leaf can promote alcohol metabolism, have protective effect to liver.Alcohol metabolism is set normally to go on smoothly.Having tea can increase Heal pipe contractile function.Theophylline has diuresis, alcohol can be promoted quickly to excrete, the harm after reduction is drunk.It drinks tea Vitamin C needed for alcohol hydrolysis can also be supplemented, the excited brain centres anaesthetized by alcohol.Thus play antialcoholism action.

The lipid-reducing function of Pu'er tea has received wide acceptance at present.But treat the effect one of mucosal lesion stomach trouble Directly supported without direct experimental data.

It is drunk since current consumption habit is only brewed with tealeaves, when functional component is brewed number, soaking temperature, brewed Between equal influence and cause dissolution difference, cannot be fully utilized.

Summary of the invention

The present invention provides a kind of medical and health care purposes that Pu'er tea is new.

The present invention also provides pharmaceutical composition containing extract of the present invention and preparation method thereof and its preparation treatment stomaches The drug of mucosa injury stomach trouble or the purposes in health food.

Pu'er tea of the invention is obtained by extracting processing, and extracting method, steps are as follows:

Step 1, puerh tea leaves add water to cook, and extracting solution filtering is concentrated to certain weight proportion；

Step 2, concentrate is centrifuged, and centrifugate concentration is drying to obtain.

Preferably, extracting method of the invention, steps are as follows:

Step 1, puerh tea leaves add water to cook extraction 2-4 times, 0.5~2 hour every time, the water of 6-12 times of volume；Extracting solution (≤70 DEG C) to tealeaves (weight) are concentrated in filtering, filtrate decompression: concentrate (volume)=1:2-1:3,

Step 2, concentrate is centrifuged with centrifuge, and centrifugate is concentrated under reduced pressure into 1.1-1.25(45-65 DEG C of specific gravity), concentration Cream is spray-dried up to (or microwave drying, crush to obtain the final product).

It is furthermore preferred that

Extracting method of the invention, steps are as follows:

Step 1, puerh tea leaves add water acutely boil decoction extract 3 times, 0.5~2 hour every time, the water of 6-12 times of volume； It is preferred that (1.5h, 1.5h, 1h；10 times of volumes, 8 times of volumes, 8 times of volumes).(≤70 DEG C) are concentrated extremely in extracting solution filtering, filtrate decompression Tealeaves (weight): concentrate (volume)=1:2-1:3,

Step 2, concentrate is centrifuged with tripod pendulum type batch centrifugal, and tripodia centrifugate is centrifuged with tube centrifuge, and centrifugate decompression is dense It is reduced to 1.1-1.25(45-65 DEG C of specific gravity), condensed cream is spray-dried up to (or microwave drying, crush to obtain the final product).

Wherein tubular type centrifugal condition: centrifuge speed: 15000-19000 turns/min；Spray drying condition: inlet air temperature: 140-190 DEG C, leaving air temp: 75-95 DEG C.

Pu'er tea of the invention includes but is not limited to the above extract, further includes having listed to purchase from market Available Pu'er tea product, such as Pu'er tea treasure, Pu'er beverage, drug and food containing Pu'er tea etc..This hair Bright Pu'er tea further includes the Pu'er tea of any art methods preparation, as Chinese patent application discloses Pu'er tea extracting method and its extract in bright book, these extracts are because all containing the functional components extracted from Pu'er tea And there is the same or similar effect.

Currently preferred Pu'er tea is that Pu'er tea is precious, is a kind of Pu'er tea system of list marketing Product are taken after can brewing, and methods for making and using same belongs to the prior art.

It can be used as the active constituent preparation with treatment stomach trouble function by the extract of the present invention that above method obtains At pharmaceutical composition or health food.Health food of the present invention includes but is not limited to: beverage, dairy products, tealeaves, cake Deng.

Mucosal lesion stomach trouble of the present invention includes but is not limited to: gastritis, gastrorrhagia, erosion, is filled at gastric ulcer Blood, oedema etc..

Active pharmaceutical ingredient in pharmaceutical composition of the invention, shared weight percent can be in the composition 0.1-99.9%, remaining is pharmaceutically acceptable carrier.Pharmaceutical composition of the invention, exists in a unit, the list Position dosage form refers to the unit of preparation, such as every of tablet, every capsule of capsule, and every bottle of oral solution, every bag of granule Deng.

Pharmaceutical composition of the invention can be any pharmaceutical dosage form, these dosage forms include: tablet, sugar coated tablet, Film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral solution, mouth containing agent, granule, electuary, ball Agent, powder, paste, sublimed preparation, suspension, pulvis, solution, injection, suppository, ointment, emplastrum, creme, spray, drop Agent, patch.Preparation of the invention, preferably peroral dosage form, such as: capsule, tablet, oral solution, granule, pill, powder, Sublimed preparation, paste etc..

Pharmaceutical composition of the invention, the preparation of oral administration can contain common excipient, such as adhesive, filling Agent, diluent, tablet agent, lubricant, disintegrating agent, colorant, flavoring agent and wetting agent when necessary can be coated tablet.

Applicable filler includes cellulose, mannitol, lactose and other similar fillers.Suitable disintegrating agent packet Include starch, polyvinylpyrrolidone and starch derivatives, such as sodium starch glycollate.Suitable lubricant includes, such as firmly Fatty acid magnesium.Suitable pharmaceutically acceptable wetting agent includes lauryl sodium sulfate.

Can be by mixing, filling, commonly method prepares solid oral composition for tabletting etc..Carrying out mixing repeatedly can make to live Property substance be distributed in entirely using in those of a large amount of fillers composition.

The form of oral liquid for example can be aqueous or oily suspensions, solution, emulsion, syrup or elixir, Or it can be a kind of dry products that can be compounded with water or other suitable carriers before use.This liquid preparation can contain Conventional additive, such as suspending agent, such as sorbierite, syrup, methylcellulose, gelatin, hydroxyethyl cellulose, carboxymethyl are fine Dimension element, aluminium stearate gel or hydrogenated edible fats, emulsifier, such as lecithin, anhydro sorbitol monooleate or Arab Glue；Non-aqueous carrier (they may include edible oil), for example, apricot kernel oil, fractionated coconut oil, such as glycerol ester oily ester, Propylene glycol or ethyl alcohol；Preservative, such as para hydroxybenzene methyl esters or propylparaben or sorbic acid, and if desired, Contain conventional flavouring agent or colorant.

For injection, the fluid unit dosage form of preparation contains active material and sterile carrier of the invention.According to carrier And concentration, this compound can be suspended or be dissolved.The preparation of solution is usually by the way that active material is dissolved in a kind of load In body, disinfection is filtered before being loaded into a kind of suitable bottle or ampoule, is then sealed.For example a kind of local anaesthesia of auxiliary material Agent, preservative and buffer are also soluble in this carrier.It, can be after being packed into bottle by this in order to improve its stability Kind composition frost, and under vacuum remove water.

Suitable pharmaceutically acceptable load is optionally added pharmaceutical composition of the invention when being prepared into medicament Body, the pharmaceutically acceptable carrier are selected from: mannitol, sorbierite, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, salt Sour cysteine, thioacetic acid, methionine, injection Vitamin B_6 DTA disodium, Ethylenediaminetetraacetic Acid Calcium Salt, carbonate, the acetic acid of monovalence alkali metal Salt, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, wheat Bud sugar, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its Derivative, alginates, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surface-active Agent, polyethylene glycol, cyclodextrin, beta-cyclodextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate etc..

Composition of the invention determines usage and dosage according to the patient's condition when in use, can often taken three times per day, each 1- 20 doses, such as: 1-20 bags or grain or piece.

The invention also includes Pu'er teas of the invention in the drug of preparation treatment mucosal lesion stomach trouble Using.

Pu'er tea of the invention has the function of good health care and treatment, especially in treatment mucosal lesion In terms of stomach trouble.

Illustrate beneficial effects of the present invention below by way of experimental data:

Pu'er tea treasure causes the protective effect of acute gastric mucosal injury to study dehydrated alcohol

The particular content of experiment is as follows:

1. experiment purpose

This research causes gastric mucosa damage model to evaluate Pu'er tea treasure to the shadow of mucosal lesion using dehydrated alcohol It rings.

Acute gastric mucosal injury is clinical common a kind of disease of digestive system, mostly in body severe trauma, shock, infection Shi Fasheng, using the erosion of stomach lining, ulcer, bleeding as main feature.This experiment is using alcohol induced acute gastric mucosal injury Animal model, ethyl alcohol are acknowledged as a kind of damage factor in animal and clinical research, and significant mucous membrane can be caused congested, bad Extremely, oedema and mucous membrane or submucosa bleeding.The formation of damage is mediated by oxygen radical.

2. materials and methods

2.1 experimental material

Experimental animal and feeding environment: SPF grades SD rat 80, male, 180~200g of weight is purchased from Beijing dimension tonneau Hua Shi zoo technical Co., Ltd.Animal freely ingests and drinks water in whole experiment process, and 18~22 DEG C of room temperature, relative humidity 50%~60%.

Test drug: Pu'er tea lot number: 2010F01.

Reagent: dehydrated alcohol is that analysis is pure, is produced by Li Anlongbohua (Tianjin) medical chemistry Co., Ltd, lot number: 20110812。

2.2 experimental method

2.2.1 experimental group

80 rats are randomly divided into: Normal group, model group, Pu'er tea low dose group (0.5g/kg), general Pu'er tea tea extraction middle dose group (1g/kg), Pu'er tea high dose group (1.5g/kg), every group 9.Each tested material is continuous 14d is given in stomach-filling, is tested in modeling in the 15th day.

2.2.2 dehydrated alcohol causes gastric mucosa damage model

Animal is deprived of food but not water for 24 hours before prepared by model, Normal group with physiological saline 1mL stomach-filling, remaining each group with Animal (blood sampling is used for the detection of biochemical indicator before putting to death) is put to death in dehydrated alcohol 1mL stomach-filling after 1h, cut open the belly ligature stomachus pyloricus and Pars cardiaca, and 10% formalin 10mL is injected into stomach, it then takes stomach to be placed in 10% formalin and fixes 20min.

2.3 observation indexs and method

1. mucosal lesion situation: fixed stomach being cut off along greater curvature, physiological saline washes away residue in stomach, and stomach is sticked Film expansion, observes and evaluates damage index.With vernier caliper measurement blutpunkte or the length and width of bleeding band.Because of width institute's generation The seriousness of table damage is big far beyond length, therefore double integral.Its standards of grading is shown in Table 1.

1 acute alcohol of table damage naked eyes, which are seen, looks into standards of grading

Evaluation index: each experimental group gastric mucosa damage degree is inhibited with the rate of injury (%), lesion extent index and damage Rate indicates.There is rat quantity/this group of rat quantity × 100% of bleeding or ulcer in the rate of injury (%)=certain group；Lesion extent Index=group Injury score summation/group size of animal；Damage inhibiting rate (%)=(A-B)/A × 100%(A, B be respectively model group with The lesion extent of test group).

2. Plasma SOD (SOD), malonaldehyde (MDA) measure: illustrating to operate by kit.

3. histopathologic examination and scoring: gross examination finishes, by the position of every gastric mucosa of animal damage most serious It cuts, is fixed on 10% formalin, conventional film-making, HE dyeing, microscopic observation.It is cut → is dehydrated → transparent → waxdip → embedding → slice → dyeing → mounting.

Instrument and reagent: long sage's JQC-A type pathologic sampling worktable；LEICA ASP300 pathological tissue dehydrating machine； LEICAEG1140C pathologic tissue packing machine；LEICA RM2145 pathologic section slicer.Ethyl alcohol, Amway Long Bohua (Tianjin) medicine have Limit company；Formaldehyde, Amway Long Bohua (Tianjin) Pharmaceuticals Ltd；Dimethylbenzene, Amway Long Bohua (Tianjin) Pharmaceuticals Ltd； Paraffin, Leca company；Haematoxylin dye liquor, Japan's force rattan Chemical Co., Ltd.；Eosin stain, Amway Long Bohua (Tianjin) doctor Medicine Co., Ltd.

Diagosis: OLYMPUS BX53 pathology microscope diagosis is used, and with OLYMPUS DP72 image capturing system to allusion quotation Type lesion is taken a picture.

The positive cross section of attentional selection gastric mucosa, the regional observation including mucous membrane holostrome.Methods of marking: it with hyperemia, bleeding, glues Theca cell degeneration necrosis is divided into 5 grades in the degree of involving of entire mucous epithelium layer.Congested weight is 1, and bleeding weight is 2, epithelium Wall regeneration weight is 3, and standards of grading and lesion total mark formula are shown in Table 2.

Standards of grading under 2 acute gastric mucosal lesion mirror of table

2.4 statistical method

It is analyzed using SPSS10.0 software, experimental result compares the conspicuousness of group difference using one-way analysis of variance.

3. experimental result

Influence of 3.1 Pu'er teas to mucosal lesion

The result shows that Pu'er tea (0.5g/kg, 1g/kg, 1.5g/kg) stomach-filling obviously inhibits in dose dependent Dehydrated alcohol causes the generation of gastric mucosa damage, and damage inhibiting rate is respectively as follows: 17.48%, 45.00%, 72.00%；Wherein high dose Amount group is better than Cimetidine group, and Pu'er tea is prompted to cause gastric mucosa damage to have apparent protection to make dehydrated alcohol With.

3 Pu'er tea treasure of table causes the influence of mucosal lesion to dehydrated alcohol

Note: * * 0.01 , ﹡ of P ﹤ P ﹤ 0.05 compared with model comparison compared with model comparison

Influence of 3.2 Pu'er teas to activity of SOD in serum and MDA content

Research shows that a plurality of types of acute gastric mucosal injuries are all related with oxygen radical, SOD and MDA are body serum In oxidation resistant index.The blood flow of the acute gastric mucosal injury Yu oxygen radical and stomach lining of verified alcohol-induced is reduced It is related.The study find that Pu'er tea treasure high dose group is improved effect to SOD in serum level and model group compares to have and significantly mentions Height, and difference has statistical significance (P < 0.01), and Pu'er tea treasure is prompted to have the function of improving oxidation resistance.It the results are shown in Table 4。

Influence of the 4 Pu'er tea treasure of table to activity of SOD in serum and MDA content

Note: * * 0.01 , ﹡ of P ﹤ P ﹤ 0.05 compared with model comparison compared with model comparison

Influence of 3.3 Pu'er teas to Pathomorphology

Normal group: rarely seen hyperemia.Model group: gastric mucosa 3/5-4/5 epithelial cell necrosis falls off, and congestion and edema locally has Bleeding, local parietal cell and chief cell degeneration necrosis, lesion area is larger, more deeply.High, normal, basic group of disease of Cimetidine group, Pu'er tea Become same model group, but lesion score is below model group.As a result list 5,6,7,8,9,10 and Fig. 2,3,4,5,6,7 are seen below.

Table 5 normally organizes gastric mucosa damage lesion score result

6 model group gastric mucosa damage lesion score result of table

7 Cimetidine group gastric mucosa damage lesion score result of table

8 Pu'er tea treasure's low dose group gastric mucosa damage lesion score result of table

9 Pu'er tea treasure's middle dose group gastric mucosa damage lesion score result of table

10 Pu'er tea treasure's high dose group gastric mucosa damage lesion score result of table

4. discussing

This research causes gastric mucosa damage model by using dehydrated alcohol, and Pu'er tea filling is given in discovery in advance Stomach can dose-dependently inhibit acute gastric mucosal injury caused by dehydrated alcohol in continuous 14 days, and inhibiting rate is from low to high Successively are as follows: 17.48%, 45.00%, 72.00%, show that Pu'er tea there is apparent protection to make acute gastric mucosal injury With.Pu'er tea treasure high dose group can significantly improve SOD content in serum to blood biochemical as the result is shown.Pathological examination results are shown The precious each dosage group of Pu'er tea has protective effect to stomach lining.

The invention has the advantages that providing a kind of Pu'er tea, extract test proves there is good treatment stomach Mucosa injury stomach trouble function, while active constituent content is high, quality is stablized, and it is easy to carry, convenient for taking, can be used for preparing medicine Product and various health foods or health protection tea play good treatment stomach trouble effect.

Detailed description of the invention

Fig. 1 is the sample photo that each experimental group influences situation on mucosal lesion.

Fig. 2 normally organizes image (400 times) under gastric mucosa damage lesion mirror

Image (400 times) under Fig. 3 model group gastric mucosa damage lesion mirror

Image (400 times) under Fig. 4 Cimetidine group gastric mucosa damage lesion mirror

Image (400 times) under Fig. 5 Pu'er tea treasure's low dose group gastric mucosa damage lesion mirror

Image (400 times) under Fig. 6 Pu'er tea treasure's middle dose group gastric mucosa damage lesion mirror

Image (400 times) under Fig. 7 Pu'er tea treasure's high dose group gastric mucosa damage lesion mirror

Specific embodiment

The present invention is further illustrated by the following examples, but not as limitation of the present invention, the water of Pu'er tea is extracted Object all has effect of the present invention.

Embodiment 1

Pu'er tea production technology:

Puerh tea leaves add water acutely boil decoction extract 3 (1.5h, 1.5h, 1h；10bv, 8bv, 8bv), extracting solution mistake Filter, filtrate decompression be concentrated (≤70 DEG C) to tealeaves (weight): concentrate (volume)=1:2-1:3, concentrate tripod pendulum type batch centrifugal from The heart, tripodia centrifugate are centrifuged with tube centrifuge, and centrifugate is concentrated under reduced pressure into 1.1-1.25(45-65 DEG C of specific gravity), condensed cream spray Mist is drying to obtain (or microwave drying, crush to obtain the final product).

Wherein tubular type centrifugal condition: centrifuge speed: 17000 turns/min；Spray drying condition: inlet air temperature: 160 DEG C, Leaving air temp: 85 DEG C.

Embodiment 2

Step 1, puerh tea leaves add water to cook extraction 3 times, 0.5 hour every time, the water of 6 times of volumes；Extracting solution filtering, filtrate It is concentrated under reduced pressure (≤70 DEG C) to tealeaves (weight): concentrate (volume)=1:2,

Step 2, concentrate is centrifuged with centrifuge, and centrifugate is concentrated under reduced pressure into 1.1(45 DEG C of specific gravity), condensed cream spray drying Up to (or microwave drying, crush to obtain the final product).

Embodiment 3

Step 1, puerh tea leaves add water to cook extraction 3 times, 2 hours every time, the water of 12 times of volumes；Extracting solution filtering, filtrate It is concentrated under reduced pressure (≤70 DEG C) to tealeaves (weight): concentrate (volume)=1:3,

Step 2, concentrate is centrifuged with centrifuge, and centrifugate is concentrated under reduced pressure into 1.25(65 DEG C of specific gravity), condensed cream is done by spraying Dry (or microwave drying, crush to obtain the final product) to obtain the final product.

Embodiment 4

Step 1, puerh tea leaves add water acutely boil decoction extract 3 times, 0.5 hour every time, the water of 6 times of volumes；Extracting solution (≤70 DEG C) to tealeaves (weight) are concentrated in filtering, filtrate decompression: concentrate (volume)=1:2,

Step 2, concentrate is centrifuged with tripod pendulum type batch centrifugal, and tripodia centrifugate is centrifuged with tube centrifuge, and centrifugate decompression is dense It is reduced to 1.1(45 DEG C of specific gravity), condensed cream is spray-dried up to (or microwave drying, crush to obtain the final product).

Wherein tubular type centrifugal condition: centrifuge speed: 15000 turns/min；Spray drying condition: inlet air temperature: 140 DEG C, Leaving air temp: 75 DEG C

Embodiment 5

Step 1, puerh tea leaves add water acutely boil decoction extract 3 times, 2 hours every time, the water of 12 times of volumes.Extracting solution mistake (≤70 DEG C) to tealeaves (weight) are concentrated in filter, filtrate decompression: concentrate (volume)=1:3,

Step 2, concentrate is centrifuged with tripod pendulum type batch centrifugal, and tripodia centrifugate is centrifuged with tube centrifuge, and centrifugate decompression is dense It is reduced to 1.25(65 DEG C of specific gravity), condensed cream is spray-dried up to (or microwave drying, crush to obtain the final product).

Wherein tubular type centrifugal condition: centrifuge speed: 19000 turns/min；Spray drying condition: inlet air temperature: 190 DEG C, Leaving air temp: 95 DEG C

Embodiment 6

Puerh tea leaves add water acutely boil decoction extract 3 (1h, 1h, 1h；6bv, 6bv, 6bv), extracting solution filtering, filtrate Be concentrated under reduced pressure (≤70 DEG C) to tealeaves (weight): concentrate (volume)=1:2.5, concentrate are centrifuged with tripod pendulum type batch centrifugal, tripodia from Heart liquid is centrifuged with tube centrifuge, and centrifugate is concentrated under reduced pressure into 1.1-1.25(50 DEG C of specific gravity), condensed cream is spray-dried to obtain the final product (or microwave drying, crush to obtain the final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turns/min；Spray drying condition: into Air temperature: 160 DEG C, leaving air temp: 85 DEG C

Embodiment 7

Puerh tea leaves add water acutely boil decoction extract 3 (2h, 2h, 2h；10bv, 10bv, 10bv), extracting solution filtering, (≤70 DEG C) to tealeaves (weight) are concentrated in filtrate decompression: concentrate (volume)=1:2.5, concentrate are centrifuged with tripod pendulum type batch centrifugal, and three Sufficient centrifugate is centrifuged with tube centrifuge, and centrifugate is concentrated under reduced pressure into 1.1-1.25(50 DEG C of specific gravity), condensed cream spray drying is Obtain (or microwave drying, crush to obtain the final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turns/min；Spray drying condition: Inlet air temperature: 160 DEG C, leaving air temp: 85 DEG C

Embodiment 8

Puerh tea leaves add water acutely boil decoction extract 2 (0.5h, 0.5h；12bv, 12bv), extracting solution filtering, filtrate Be concentrated under reduced pressure (≤70 DEG C) to tealeaves (weight): concentrate (volume)=1:2.5, concentrate are centrifuged with tripod pendulum type batch centrifugal, tripodia from Heart liquid is centrifuged with tube centrifuge, and centrifugate is concentrated under reduced pressure into 1.1-1.25(50 DEG C of specific gravity), condensed cream is spray-dried to obtain the final product (or microwave drying, crush to obtain the final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turns/min；Spray drying condition: into Air temperature: 160 DEG C, leaving air temp: 85 DEG C

Embodiment 9

Puerh tea leaves add water acutely boil decoction extract 4 (2h, 2h, 2h, 2h；10bv, 10bv, 10bv, 10bv), it extracts (≤70 DEG C) to tealeaves (weight) are concentrated in liquid filtering, filtrate decompression: concentrate (volume)=1:2.5, concentrate tripod pendulum type batch centrifugal Centrifugation, tripodia centrifugate are centrifuged with tube centrifuge, and centrifugate is concentrated under reduced pressure into 1.1-1.25(50 DEG C of specific gravity), condensed cream spray Mist is drying to obtain (or microwave drying, crush to obtain the final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turns/min；It is spraying Drying condition: inlet air temperature: 160 DEG C, leaving air temp: 85 DEG C

Embodiment 10

According to any one extract in embodiment 1-9, customary adjuvant is added, using the conventional method on galenic pharmacy, system At tablet.

Embodiment 11

According to any one extract in embodiment 1-9, customary adjuvant is added, using the conventional method on galenic pharmacy, system At capsule.

Embodiment 12

According to any one extract in embodiment 1-9, customary adjuvant is added, using the conventional method on galenic pharmacy, system At granule.

Embodiment 13

According to any one extract in embodiment 1-9, customary adjuvant is added, using the conventional method on galenic pharmacy, system At powder.

Embodiment 14

According to any one extract in embodiment 1-9, customary adjuvant is added, using the conventional method on galenic pharmacy, system At pill.

Embodiment 15

According to any one extract in embodiment 1-9, customary adjuvant is added, using the conventional method on galenic pharmacy, system At injection.

Embodiment 16

It according to any one extract in embodiment 1-9, is added in tea product, is made by a certain percentage with common process At the health protection tea with protection gastric mucosa function.

Embodiment 17

According to any one extract in embodiment 1-9, it is added in dairy products, is made by a certain percentage with common process Health food with protection gastric mucosa function.

Embodiment 18

According to any one extract in embodiment 1-9, it is added in beverage, is made by a certain percentage with common process Health drink with protection gastric mucosa function.

Embodiment 19

According to any one extract in embodiment 1-9, it is added in cake, is made by a certain percentage with common process Health care cake with protection gastric mucosa function.

Claims (6)

1. a kind of application of Pu'er tea in the drug of preparation treatment mucosal lesion stomach trouble, the Pu'er tea extract Object, extraction step are as follows:

Step 1, puerh tea leaves add water to cook extraction 2-4 times, 0.5~2 hour every time, the water of 6-12 times of volume；Extracting solution filtering, Filtrate is concentrated under reduced pressure into tealeaves weight under the conditions of≤70 DEG C: the volume of the concentrated liquid=1:2-1:3,

Step 2, concentrate is centrifuged with centrifuge, and centrifugate is concentrated under reduced pressure into 45-65 DEG C of specific gravity 1.1-1.25, and condensed cream is spraying Dry or microwave drying to get；Wherein, the mucosal lesion stomach trouble includes: gastritis, gastric ulcer, gastrorrhagia, erosion, fills Blood, oedema.

2. application according to claim 1, which is characterized in that the Pu'er tea, extraction step are as follows:

Step 1, puerh tea leaves add water acutely boil decoction extract 3 times, 0.5~2 hour every time, 6-12 times of volume of amount of water Water；Extracting solution filtering, filtrate are concentrated under reduced pressure into tealeaves weight under the conditions of≤70 DEG C: the volume of the concentrated liquid=1:2-1:3,

Step 2, concentrate is centrifuged with tripod pendulum type batch centrifugal, and tripodia centrifugate is centrifuged with tube centrifuge, and centrifugate is concentrated under reduced pressure into 45-65 DEG C of specific gravity 1.1-1.25, condensed cream spray drying or microwave drying to get；

Wherein tubular type centrifugal condition: centrifuge speed: 15000-19000 turns/min；Spray drying condition: inlet air temperature: 140- 190 DEG C, leaving air temp: 75-95 DEG C.

3. application according to claim 1, which is characterized in that the Pu'er tea, extraction step are as follows:

Puerh tea leaves add water acutely boil decoction extract 3 times, the 1st decoction 1.5h adds 10 times of volumes of water；2nd decoction 1.5h, Add 8 times of volumes of water；3rd decoction 1h, adds 8 times of volumes of water, extracting solution filtering, and filtrate is concentrated under reduced pressure into tea under the conditions of≤70 DEG C Leaf weight: the volume of the concentrated liquid=1:2-1:3, concentrate are centrifuged with tripod pendulum type batch centrifugal, and tripodia centrifugate is centrifuged with tube centrifuge, Centrifugate is concentrated under reduced pressure into 45-65 DEG C of specific gravity 1.1-1.25, condensed cream spray drying or microwave drying to get；

Wherein tubular type centrifugal condition: centrifuge speed: 15000-19000 turns/min；Spray drying condition: inlet air temperature: 140- 190 DEG C, leaving air temp: 75-95 DEG C.

4. application according to claim 1, which is characterized in that the application includes taking Pu'er tea as pharmaceutical activity The pharmaceutical composition of ingredient.

5. application according to claim 4, which is characterized in that described pharmaceutical composition is any pharmaceutical dosage form.

6. application according to claim 5, which is characterized in that described pharmaceutical composition dosage form is selected from: tablet, capsule, particle Agent, pill, pulvis, solution.