CN102641342B - A kind of Chinese medicine extract and preparation method for the treatment of nephropathy - Google Patents
A kind of Chinese medicine extract and preparation method for the treatment of nephropathy Download PDFInfo
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- CN102641342B CN102641342B CN201110038081.0A CN201110038081A CN102641342B CN 102641342 B CN102641342 B CN 102641342B CN 201110038081 A CN201110038081 A CN 201110038081A CN 102641342 B CN102641342 B CN 102641342B
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Abstract
The invention discloses a kind of Chinese medicine extract and preparation method for the treatment of nephropathy, belong to modern Chinese traditional medicine field.Treat a Chinese medicine extract for nephropathy, this Radix Rumicis extract is obtained through extracting by Radix Rumicis medical material or decoction pieces.Result of study of the present invention display, Radix Rumicis extract, to brightic treatment, in diuresis, improves albuminuria, improves in hematuria and all show good activity.
Description
Technical field
The invention belongs to modern Chinese traditional medicine field, relate to the novelty teabag of a kind of Chinese medicine and extract thereof, be specifically related to a kind of Chinese medicine extract for the treatment of nephropathy and preparation method thereof.
Background technology
Radix Rumicis has another name called Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) among the people, Radix Rumicis Crispi, Radix Rumicis Patientiae etc., for multiple in Polygonaceae Rumex (Rumex) can medicinal plants root and rhizome.Mainly contain Radix Rumicis Japonici, Rumex patientia Linn., Nepal's Radix rumicis acetosae etc., among the people with medical herbs use, there is heat-clearing and toxic substances removing, cooling blood for hemostasis, the effects such as killing parasites for relieving itching.Radix Rumicis aboundresources, but the domestic and international research for Radix Rumicis is also very deficient at present, waits us and goes further to excavate.
The key agents effect of document to Radix Rumicis is summarized as follows: (1) anastalsis: Radix Rumicis has promotion blood coagulation, reduces vascular fragility, strengthens the effect of blood capillary contractility.This product decoct can make mice blood clotting time obviously shorten, and is tested by blood capillary tube method, proves that it can make blood capillary shrink, and permeability reduces.The test of Bufo siccus systemic perfusion shows that it can make vasoconstriction.Stop blooding in Rumex chalepensis Mill composition Polygonum runcinatum var. sinense Hemsl. A prime, its anastalsis system shrinks by directly making small artery, promotes the generation of bioprotein composition, and may be relevant with promotion progestogen action.The effect of wooden Quercetin and the wooden Quercitroside still enhancing capillary resistance that tool is good in net fruit acid mould.(2) eliminate the phlegm, cough-relieving and antiasthmatic effect: after Radix Rumicis root water decoction Deproteinization, decocting liquid all has obvious expelling phlegm for arresting cough effect to mouse stomach; Emodin, chrysophanic acid antitussive action are obvious; General anthraquinone has obvious antiasthmatic effect to Cavia porcellus gavage, and data shows that the nubbin after extracting emodin, chrysophanol and a large amount of acid also has antiasthmatic effect.(3) antibacterial actions; Emodin in this product root, chrysophanic acid, chrysophanol have inhibitory action in various degree to alpha streptococcus, streptococcus pneumoniae, hemophilus influenza and micrococcus catarrhalis in test tube.In addition, the in vitro tests of bibliographical information emodin also has inhibitory action in various degree to staphylococcus aureus, escherichia coli.The inhibitory action of Rumex gmelini Turcz. infusion to staphylococcus aureus is only second to oxytetracycline, identical with oxytetracycline to the inhibition of group B streptococcus, diphtheria corynebacterium.The net fruit ethanol extraction of Folium rumicis acetosae and fresh leaf juice all have comparatively high inhibition effect to bacillus ambiguus and Salmonella paratyphi A, salmonella typhi.(4) antifungal, antivirus action: the dermatosis that the rumicin in Radix Rumicis root acts on red trichophyta, trichophyton interdigitalis and fungal infection is respond well.Bibliographical information root tincture has inhibitory action to Microspore and megaspore mycete on husky Bo Shi nutrient chemical, and minimal effective concentration is 1.56-3.12%.Wood Quercitroside and Asia influenza A virus inject Embryo Gallus domesticus, have prevention infection effect.(5) antitumor action: curled dock extract, experiment finds there is anti-evil effect to tumor, and acidic drug effect is stronger.(6) other effect: data shows anthraquinone derivative tool laxative action, and vitamin B1 can be used as gastrotonica, analeptic; This product also has effect of certain solution pain, calmness, diuresis, parasite killing.Decoct ethanol concentration extraction is inhibited to acute lymphoblastic leukemia, Acute monocytic type leukemia and subacute type leukaemic hemocyte dehydrogenase, analyzes and finds that its dialogue Cellular respiration has inhibitory action.Except this, this product can also increase appetite, improving water flood, raising body resistance against diseases.
At present, bibliographical information Radix Rumicis extract is not had to be used for the treatment of the purposes of nephropathy.
Summary of the invention
The technical problem to be solved in the present invention is to provide that a kind of therapeutic effect is good, dosage is few, the medicine of the treatment nephropathy of few side effects.
Medicine of the present invention is Chinese medicine Radix Rumicis extract.
Containing effective ingredient Radix Rumicis general anthraquinone in Radix Rumicis extract of the present invention, its mass percentage content in extract is 50-90%.
The extracting mode of Radix Rumicis of the present invention is selected from: reflux extraction, steam distillation, supercritical extraction, decocting method, infusion process, percolation, alcohol extraction, water extraction are made.
Extract of the present invention is made up through alcohol extraction and water extraction of Chinese medicine Radix Rumicis.
Alcohol of the present invention is ethanol.
Radix Rumicis extract of the present invention, preferred preparation method is as follows:
(1) fetch earth rhubarb medicinal material or decoction pieces, with 4-8 times amount 60-95% ethanol extraction 2-3 time, and each 1-3 hour;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 6-10 times amount water extraction 2-3 time, each 1-3 hour, merges Aqueous extracts;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 1-5 times amount and the 5-20% of resin bed volume 2-7 times amount successively, finally use resin bed volume 8-12 times amount 70-95% ethanol elution, collect 70-95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 5-10% sodium hydroxide solution again, filter, filtrate adjust pH, to 1-4, is placed 12-24 hour for less than 10 DEG C, is filtered, must precipitate drying, to obtain final product.
Preferred further, Radix Rumicis extract of the present invention, preparation method is as follows:
(1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times amount 90% ethanol extraction 2 times, each 2 hours, filters;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 8 times amount water extraction 2 times, each 1.5 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the water of resin bed volume 2.5 times amount and 15% ethanol elution of resin bed volume 5 times amount successively, finally use the ethanol elution of 95% of resin bed volume 10 times amount, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 6% sodium hydroxide solution again, filter, filtrate adjust pH to 2.5, place 12-16 hour for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
Other preferred preparation methoies of the present invention in an embodiment.
Another object of the present invention is to provide a kind of Radix Rumicis extract standby in order to top legal system.
Another object of the present invention is to provide a kind of pharmaceutical composition containing Radix Rumicis extract.
Pharmaceutical composition of the present invention, can contain medicine acceptable carrier as required, wherein using Radix Rumicis extract as active constituents of medicine, its in the formulation shared percentage by weight can be 0.1-99.9%, all the other are medicine acceptable carrier.Pharmaceutical preparations composition of the present invention, exists in a unit, and described unit dosage form refers to the unit of preparation, as every sheet of tablet, and every capsules of capsule, every bottle of oral liquid, granule every bag, often propping up of injection.
Pharmaceutical composition of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, syrup, suck agent, granule, electuary, pill, drop pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.
Preferably, compositions of the present invention is oral formulations or injection.
Wherein, described oral formulations is selected from the one in capsule, tablet, drop pill, granule, concentrated pill, oral liquid and mixture.
Wherein, described injection is selected from the one in injection, lyophilized injectable powder and aqueous injection.
Pharmaceutical composition of the present invention, the preparation of its oral administration can containing conventional excipient, and such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet if desired.
The filler be suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, such as sodium starch glycollate.Suitable lubricant comprises, such as magnesium stearate.The suitable acceptable wetting agent of medicine comprises sodium lauryl sulphate.
Pharmaceutical composition of the present invention, by mixing, is filled, and the method that tabletting etc. are conventional prepares solid oral composition.Repeatedly mix and active substance can be made to be distributed in those compositionss of a large amount of filler of whole use.
The form of oral liquid can be such as aqueous or oily suspensions, solution, Emulsion, syrup or elixir, or can be the composite dry products of a kind of available water before use or other suitable carrier.This liquid preparation can containing conventional additive, such as suspending agent, such as sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agent, such as lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), the oily ester of the such as ester of almond oil, fractionated coconut oil, such as glycerol, propylene glycol or ethanol; Antiseptic, such as para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if need, can containing conventional flavouring agent or coloring agent.
For injection, the fluid unit dosage form of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this compound can be suspended or dissolve.The preparation of solution is normally by being dissolved in a kind of carrier by active substance, filter-sterilized before being loaded a kind of suitable bottle or ampoule, then seals.Adjuvant such as a kind of local anesthetic, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, by freezing for this compositions after loading bottle, and under vacuo water can be removed.
Pharmaceutical composition of the present invention, applicable medicine acceptable carrier is optionally added when being prepared into medicament, described medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its derivates, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Another object of the present invention is to provide the application in the medicine of preparation treatment nephropathy of raw material of Chinese medicine Radix Rumicis or its extract.
Present invention also offers a kind of pharmaceutical composition containing Radix Rumicis or its extract and prepare the application in the medicine for the treatment of nephropathy.
Nephropathy of the present invention comprises: chronic glomerulonephritis, acute glomerulonephritis, latent nephritis, henoch Schonlein purpura nephritis, nephrotic syndrome, diabetic nephropathy, Lupus nephritis, renal failure, IgA nephropathy and non-IgA nephropathy.
The content assaying method additionally providing Radix Rumicis general anthraquinone of the present invention.
In Radix Rumicis extract of the present invention, the mass percentage content of effective ingredient Radix Rumicis general anthraquinone is 50-90%.
Assay of Total Anthraquinone method in Radix Rumicis extract of the present invention: get extract appropriate, put in flask, add the hydrochloric acid solution 10ml of 8%, supersound process 2min, add chloroform 10ml again, reflux 1 hour, let cool, put in separatory funnel, with a small amount of chloroform washing container, divide and get chloroform layer, acid solution uses chloroform extraction 3 times again, each 10ml, merge chloroform liquid, decompression and solvent recovery is to dry, residue adds methanol makes dissolving, be transferred in 10ml measuring bottle, add methanol and put scale, shake up, microporous filter membrane filters to obtain need testing solution, with certain density chrysophanol, emodin, physcione reference substance solution for contrast, under 254nm wavelength, HPLC method measures the content of three in need testing solution, and three's content sum is the content of general anthraquinone in extract.
We have done the test of pesticide effectiveness to Radix Rumicis of the present invention or its extract, and result of study shows, and Radix Rumicis of the present invention or its extract have good effect in brightic treatment, in diuresis, improve albuminuria, improve in hematuria and all show good activity, result of the test also shows, and medication effect of the present invention is remarkable, and dosage is few, instant effect, few side effects, good stability, preparation technology is simple, with low cost, be applicable to large-scale production.
Detailed description of the invention
By the more detailed explanation the present invention of following specific embodiment, but not as restriction of the present invention.
Embodiment 1, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times amount 90% ethanol extraction 2 times, each 2 hours, filters;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 8 times amount water extraction 2 times, each 1.5 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the water of resin bed volume 2.5 times amount and 15% ethanol elution of resin bed volume 5 times amount successively, finally use 95% ethanol elution of resin bed volume 10 times amount, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A
(5) dissolve with appropriate 6% sodium hydroxide solution again, filter, filtrate adjust pH to 2.5, place 12-16 hour for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 80.1%.
Embodiment 2, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 4 times amount 60% ethanol extraction 2 times, and each 1 hour;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 6 times amount water extraction 2 times, each 1 hour, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 1 times amount and 5% of resin bed volume 2 times amount successively, finally use resin bed volume 8 times amount 70% ethanol elution, collect 70% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 5% sodium hydroxide solution again, filter, filtrate adjust pH to 1, place 12 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 50.4%.
Embodiment 3, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 8 times amount 95% ethanol extraction 3 times, and each 3 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 10 times amount water extraction 3 times, each 3 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 5 times amount and 20% of resin bed volume 7 times amount successively, finally use resin bed volume 12 times amount 95% ethanol elution, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 5% sodium hydroxide solution again, filter, filtrate adjust pH to 4, place 14 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 90.0%.
Embodiment 4, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 4 times amount 85% ethanol extraction 2 times, and each 1 hour;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 6 times amount water extraction 2 times, each 2 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 1 times amount and 5% of resin bed volume 2 times amount successively, finally use resin bed volume 8 times amount 70% ethanol elution, collect 70% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 5% sodium hydroxide solution again, filter, filtrate adjust pH to 1, place 16 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 65.5%.
Embodiment 5, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 8 times amount 75% ethanol extraction 3 times, and each 3 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 10 times amount water extraction 3 times, each 3 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 5 times amount and 20% of resin bed volume 7 times amount successively, finally use resin bed volume 12 times amount 95% ethanol elution, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 5% sodium hydroxide solution again, filter, filtrate adjust pH to 4, place 18 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 61.3%.
Embodiment 6, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times amount 85% ethanol extraction 2 times, and each 2 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 8 times amount water extraction 2 times, each 2 hours, Aqueous extracts is merged;
(3) merge alcohol extract, be concentrated into without alcohol taste, add appropriate water precipitation, filter, obtain precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 3 times amount and 10% of resin bed volume 4 times amount successively, finally use resin bed volume 10 times amount 85% ethanol elution, collect 85% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 7% sodium hydroxide solution again, filter, filtrate adjust pH to 2, place 20 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 72.6%.
Embodiment 7, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times amount 85% ethanol extraction 2 times, and each 2 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 8 times amount water extraction 2 times, each 2 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 3 times amount and 10% of resin bed volume 4 times amount successively, finally use resin bed volume 10 times amount 85% ethanol elution, collect 85% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 7% sodium hydroxide solution again, filter, filtrate adjust pH to 3, place 24 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 70.8%.
Embodiment 8, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 5 times amount 95% ethanol extraction 3 times, and each 2 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 7 times amount water extraction 2 times, each 2 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 3 times amount and 15% of resin bed volume 5 times amount successively, finally use resin bed volume 11 times amount 95% ethanol elution, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 7% sodium hydroxide solution again, filter, filtrate adjust pH to 1, place 14 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 85.6%.
Embodiment 9, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 5 times amount 95% ethanol extraction 3 times, and each 2.5 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 7 times amount water extraction 2 times, each 2.5 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 3 times amount and 15% of resin bed volume 5 times amount successively, finally use resin bed volume 11 times amount 85% ethanol elution, collect 85% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 9% sodium hydroxide solution again, filter, filtrate adjust pH to 1, place 14 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 80.2%.
Embodiment 10, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 5 times amount 95% ethanol extraction 3 times, and each 1.5 hours;
(2) after ethanol flung to by alcohol extraction medicinal residues, with 9 times amount water extraction 2 times, each 1.5 hours, Aqueous extracts is merged;
(3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A,
(4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 3 times amount and 15% of resin bed volume 6 times amount successively, finally use resin bed volume 11 times amount 95% ethanol elution, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A;
(5) dissolve with appropriate 5% sodium hydroxide solution again, filter, filtrate adjust pH to 2, place 16 hours for less than 10 DEG C, filter, drying must be precipitated, to obtain final product.
After measured, in extract, the content of Radix Rumicis general anthraquinone is 83.1%.
Embodiment 11, capsule of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate lactose and make capsule.
Embodiment 12, tablet of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate dextrin and make tablet.
Embodiment 13, granule of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate carboxymethyl cellulose, 60 DEG C of forced air dryings, granulate, granulate, obtains granule.
Embodiment 14, drop pill of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate Polyethylene Glycol, mix homogeneously, melting, upper pill dripping machine, makes drop pill.
Embodiment 15, oral cavity disintegration tablet of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add 5% polyvinylpolypyrrolidone, the magnesium stearate of 0.1%, the microcrystalline Cellulose of 50%, make soft material with ethanol in proper amount solution, granulate, 60 DEG C of forced air dryings, granulate, granulate, tabletted, obtains oral cavity disintegration tablet.
Embodiment 16, capsule of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate amount of starch, sucrose and magnesium stearate, granulate, incapsulate, obtain capsule.
Embodiment 17, tablet of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate amount of starch, sodium carboxymethyl cellulose, Pulvis Talci mix homogeneously, granulate, namely tabletting obtains tablet.
Embodiment 18, oral liquid of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate syrup 4g, be dissolved in the pure water of 100ml, homogenizing, filter, through high-temperature short-time sterilization (135 DEG C, 4s).Sterile filling, subpackage, obtained oral liquid.
Embodiment 19, injectable powder of the present invention
Get any 1 Radix Rumicis extract in embodiment of the present invention 1-10, add appropriate glucose 4.5g, sodium thiosulfate 0.9g and distilled water 1ml, after said components mix homogeneously, lyophilization, subpackage, obtains injectable powder.
The pharmacodynamic experiment of embodiment 20, extract of the present invention
Zoopery
Test the impact of a Radix Rumicis extract on normal rat urine amount
[experiment material]
1. laboratory animal: SPF level SD rat 70, male, body weight 180 ~ 220g.Be purchased from Beijing company of dimension tonneau China.Rearing conditions: raise in air-conditioned animal housing, temperature is 20 DEG C ~ 25 DEG C, relative humidity 60%, 5, every cage, lighting hours 12 hours, adds feedstuff at regular time and quantity, edible Mus special feed (production of Beijing Ke Aoxie feed corporation,Ltd), freely drink water, every day changes bedding and padding
2. main agents and medicine:
2.1 reagent: normal saline: Tianjin Jin Lan pharmaceutcal corporation, Ltd, lot number: 09110904
2.2 medicines:
1. test medicine: Radix Rumicis extract (extract that the embodiment of the present invention 1 prepares)
2. positive drug: hydrochlorothiazide; Tianjin Lisheng Pharmaceutical Co., Ltd.; Lot number: 0711018
3. experimental apparatus:
Metabolic cage: Suzhou City Feng Laboratory Animal Equipment Co., Ltd
[experimental technique]
1. preliminary election: get the healthy male rat of 180g-200gSD, adapt to raising 1 day, before experiment, fasting can't help water 24 hours, and after gavage gives 25ml/kg normal saline, collect 2 hours urine volume, more than 40% person reaching gavage amount with voided volume is selected into experiment.
2. divide into groups:
Get the qualified rat of preliminary election by body weight random packet, be respectively blank group, Radix Rumicis extract high and low dose group, and hydrochlorothiazide group (positive controls), often organize 10.Dosage gets according to the clinical administration dose lonvestion of Radix Rumicis and hydrochlorothiazide tablet, is respectively each preparation high dose group 1000mg/kg, each preparation low dose group 500mg/kg, hydrochlorothiazide group 16mg/kg, blank group gavage normal saline.
3. diuresis experiment: gavage gives the 50ml/kg Normal saline loading comprising test medicine before experiment, compeling rat hypogastric region with hand makes it drain remaining urine, Normal group (namely blank group) gives corresponding normal saline, put into metabolic cage, one, every cage, collection urine per hour once, continuous 5 hours, records urine volume per hour and total volume of urine.
4. statistical method:
When initial data arranges, with 95% confidence interval definition abnormal data, exceed by numerical value
extraneous data are rejected.
SPSS11.5 software is adopted to carry out statistical analysis.Measurement data with
represent that each medicine group is compared with model group, adopt one factor analysis of variance, to evaluate the effect of medicine.P < 0.05 has significant for difference.
[experimental result]
1. compare with blank group, positive drug hydrochlorothiazide significantly can increase rat urine volume (P < 0.01); Radix Rumicis extract high dose group obviously can increase the rat voided volume of 5 hours, and difference has statistical significance (P < 0.01); Though low dose group rat urine volume increases more to some extent with blank group, difference does not have statistical significance.Concrete outcome refers to table 1
Table 1 Radix Rumicis extract is on the impact of normal rat urine amount
Compare with blank group:
*: P < 0.05;
*: P < 0.01
Test the impact of two Radix Rumicis extracts on glycerol induced Acute renal failure rat urine volume
[experiment material]
1. laboratory animal: SPF level SD rat 70, male, body weight 180 ~ 220g.Be purchased from Beijing company of dimension tonneau China.Rearing conditions: raise in air-conditioned animal housing, temperature is 20 DEG C ~ 25 DEG C, relative humidity 60%, 5, every cage, lighting hours 12 hours, adds feedstuff at regular time and quantity, edible Mus special feed (production of Beijing Ke Aoxie feed corporation,Ltd), freely drink water, every day changes bedding and padding
2. main agents and medicine:
2.1 reagent:
1. glycerol: Tianjin chemical reagent company limited; Analytical pure; Lot number: 20070312
2. normal saline: Tianjin Jin Lan pharmaceutcal corporation, Ltd, lot number: 09110904
2.2 medicines:
1. test medicine: Radix Rumicis extract (extract that the embodiment of the present invention 1 prepares)
2. positive drug: hydrochlorothiazide; Tianjin Lisheng Pharmaceutical Co., Ltd.; Lot number: 0711018
3. experimental apparatus:
Metabolic cage: Suzhou City Feng Laboratory Animal Equipment Co., Ltd
[experimental technique]
1. preliminary election: with experiment one
2. administration and method for preparation of drug: with experiment one
3. diuresis experiment: each group rat intramuscular injection 50% glycerol normal saline solution 10ml/kg modeling respectively, fasting after modeling (can't help water), after 24 hours, gavage gives the 50ml/kg Normal saline loading comprising test medicine, compeling rat hypogastric region with hand makes it drain remaining urine, and model group gives corresponding normal saline, puts into metabolic cage, one, every cage, collection urine per hour once, continuous 5 hours, records urine volume per hour and total volume of urine.
4. statistical method: with experiment one
[experimental result]
Compare with model group, each group urine volume increases all to some extent, and difference has significant, and prompting medicine has certain diuresis to rat under pathological state, but the difference between each administration group does not have statistical significance.Concrete data refer to table 2
Table 2 Radix Rumicis extract is on the impact of glycerol induced Acute renal failure rat urine amount
Compare with model group:
*: P < 0.05;
*: P < 0.01
Test the impact of three Radix Rumicis extracts on acute glomerulonephritis rat urine albumen and hematuria
[experiment material]
1. laboratory animal: SPF level SD rat 100, male and female half and half, body weight 180 ~ 220g.Be purchased from Beijing company of dimension tonneau China.Rearing conditions: raise in air-conditioned animal housing, temperature is 20 DEG C ~ 25 DEG C, relative humidity 60%, 5, every cage, lighting hours 12 hours, adds feedstuff at regular time and quantity, edible Mus special feed (production of Beijing Ke Aoxie feed corporation,Ltd), freely drink water, every day changes bedding and padding
2. main agents and medicine:
2.1 reagent:
1. normal saline: Tianjin Jin Lan pharmaceutcal corporation, Ltd, lot number: 09110904
2. Freund's complete adjuvant: sigma company, CAS:9007-81-2
3. urine protein measures test kit: Bioengineering Research Institute is built up in Nanjing, lot number: 20090424
2.2 medicines:
1. test medicine: Radix Rumicis extract (extract that the embodiment of the present invention 1 prepares)
2. positive drug: dexamethasone; Tianjin Lisheng Pharmaceutical Co., Ltd.; Lot number: 0812026
3. experimental apparatus:
1. metabolic cage: Suzhou City Feng Laboratory Animal Equipment Co., Ltd
2. urine analyzer: Roche Holding Ag MiditronJunior II
3. ultraviolet spectrophotometer: Beijing Rayleigh Analytical Instrument Co., Ltd, UV-1800
[experimental technique]
1. the preparation of renal cortex homogenate:
Use 10% chloral hydrate (0.35ml/100g) intraperitoneal injection of anesthesia animal; The rat of having anaesthetized shears is cut off both sides hind leg femoral artery and Thoracic Limb Arteries, and abundant blood-letting, to facilitate subsequent operation.Aseptically open abdomen, ligation renal artery, self-ligating place is to kidney intubate, cut off renal veins, normal saline through intubate pre-cooling rinses kidney repeatedly in white, take off kidney, the electronic high-speed homogenization machine of bark fetching matter grinds to form homogenate in ice bath, is sealed in-70 DEG C of refrigerator and cooled and freezes stand-by after subpackage.
2. the foundation of acute glomerulonephritis model:
Get 25 grams of cortex homogenate, add Freund's complete adjuvant to 50ml, be placed in mortar and grind well, slowly add normal saline 100ml, limit edged grinds, and makes emulsifying completely.Get emulsifying agent, select and get the rat of kidney rat with strain and carry out lumbar injection, dosage be each 2ml/ only, injection in every two weeks 1 time, injection 7 times, can obtain Nephritis Rats model altogether.
3. grouping and administration:
After final injection, measure the urine protein content of each rat respectively, the standard compliant rat of screening urine protein content enters group.Qualified rat is divided into 3 groups, is respectively model group, Radix Rumicis extract group (1000mg/kg) and dexamethasone (positive drug, 2mg/kg) group, every day gavage once, successive administration 14 days.
4. draw materials:
Be placed in metabolic cage in last administration, collect twenty-four-hour urine amount, according to test kit description and instrument operation instruction, measure the frequency that hematuria appears in rat urine protein content and each group respectively.
5. statistical method: with experiment one
[experimental result]
1. on the impact of urine protein and hematuria: compare with model group, positive drug dexamethasone and Radix Rumicis extract all can reduce the content of rat 24h urine protein, and difference has statistical significance (P < 0.01).Compare with model group, Radix Rumicis extract can reduce the occurrence rate of hematuria.Concrete data refer to table 3
Table 3 Radix Rumicis extract is on the impact of acute glomerulonephritis rat urine albumen and hematuria
Compare with model group:
*: P < 0.01.
Claims (4)
1. treat a Radix Rumicis extract for nephropathy, its preparation method is as follows: (1) fetch earth rhubarb medicinal material or decoction pieces, with 4-8 times amount 60-95% ethanol extraction 2-3 time, and each 1-3 hour; (2) after ethanol flung to by alcohol extraction medicinal residues, with 6-10 times amount water extraction 2-3 time, each 1-3 hour, merges Aqueous extracts; (3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A; (4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 1-5 times amount and the 5-20% of resin bed volume 2-7 times amount successively, finally use resin bed volume 8-12 times amount 70-95% ethanol elution, collect 70-95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A; (5) dissolve with appropriate 5-10% sodium hydroxide solution again, filter, filtrate adjust pH, to 1-4, is placed 12-24 hour for less than 10 DEG C, is filtered, must precipitate drying, to obtain final product.
2. Radix Rumicis extract according to claim 1, is characterized in that, preparation method is as follows: (1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times amount 90% ethanol extraction 2 times, each 2 hours, filters, (2) after ethanol flung to by alcohol extraction medicinal residues, with 8 times amount water extraction 2 times, each 1.5 hours, Aqueous extracts is merged, (3) alcohol extract is merged, filter, filtrate is concentrated into without alcohol taste, add appropriate water precipitation, filter, obtain precipitate A, (4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the water of resin bed volume 2.5 times amount and 15% ethanol elution of resin bed volume 5 times amount successively, finally use the ethanol elution of 95% of resin bed volume 10 times amount, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A, (5) dissolve with appropriate 6% sodium hydroxide solution again, filter, filtrate adjust pH to 2.5, place 12-16 hour for less than 10 DEG C, filter, drying must be precipitated, obtain.
3. treat a preparation method for the Radix Rumicis extract of nephropathy, its preparation method is as follows: (1) fetch earth rhubarb medicinal material or decoction pieces, with 4-8 times amount 60-95% ethanol extraction 2-3 time, and each 1-3 hour; (2) after ethanol flung to by alcohol extraction medicinal residues, with 6-10 times amount water extraction 2-3 time, each 1-3 hour, merges Aqueous extracts; (3) merge alcohol extract, filter, filtrate is concentrated into without alcohol taste, adds appropriate water precipitation, filters, obtains precipitate A; (4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the ethanol elution of the water of resin bed volume 1-5 times amount and the 5-20% of resin bed volume 2-7 times amount successively, finally use resin bed volume 8-12 times amount 70-95% ethanol elution, collect 70-95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A; (5) dissolve with appropriate 5-10% sodium hydroxide solution again, filter, filtrate adjust pH, to 1-4, is placed 12-24 hour for less than 10 DEG C, is filtered, must precipitate drying, to obtain final product.
4. preparation method according to claim 3, is characterized in that, preparation method is as follows: (1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times amount 90% ethanol extraction 2 times, each 2 hours, filters, (2) after ethanol flung to by alcohol extraction medicinal residues, with 8 times amount water extraction 2 times, each 1.5 hours, Aqueous extracts is merged, (3) alcohol extract is merged, filter, filtrate is concentrated into without alcohol taste, add appropriate water precipitation, filter, obtain precipitate A, (4) supernatant and Aqueous extracts merge and filter, filtrate crosses polyamide chromatography post, use the water of resin bed volume 2.5 times amount and 15% ethanol elution of resin bed volume 5 times amount successively, finally use the ethanol elution of 95% of resin bed volume 10 times amount, collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A, (5) dissolve with appropriate 6% sodium hydroxide solution again, filter, filtrate adjust pH to 2.5, place 12-16 hour for less than 10 DEG C, filter, drying must be precipitated, obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110038081.0A CN102641342B (en) | 2011-02-15 | 2011-02-15 | A kind of Chinese medicine extract and preparation method for the treatment of nephropathy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110038081.0A CN102641342B (en) | 2011-02-15 | 2011-02-15 | A kind of Chinese medicine extract and preparation method for the treatment of nephropathy |
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| CN104224951A (en) * | 2014-09-28 | 2014-12-24 | 江西百神药业股份有限公司 | Total anthraquinones with stable and uniform proportions of various components and composition of total anthraquinones for treating cholecystitis |
| CN104224953A (en) * | 2014-09-28 | 2014-12-24 | 江西百神昌诺药业有限公司 | Total anthraquinones with stable and uniform proportions of various components and composition of total anthraquinones for treating chronic renal failure |
| CN104224952A (en) * | 2014-09-28 | 2014-12-24 | 江西百神昌诺药业有限公司 | Preparation method for total anthraquinones of rheum officinale with stable and uniform proportions of all components |
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Non-Patent Citations (4)
| Title |
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| 土大黄治疗小儿急性肾功能衰竭;孙清政;《中国乡村医生杂志》;20011231(第3期);第27页左栏第2段 * |
| 土大黄研究进展;丁力晓;《甘肃中医》;19981231;第11卷(第6期);第40页左栏第2段 * |
| 大黄酚和大黄酸的合成;马燕如等;《合成化学》;20071231;第15卷(第2期);第244页左栏倒数第9行 * |
| 武当山5种土大黄中3种蒽醌类成分的含量比较;王雪芹等;《医药导报》;20100903;第29卷(第9期);第127页左栏2.2.2 * |
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