CN103505564B - A kind of application of Pu-Er ripe tea extract in blood-pressure drug or food is prepared - Google Patents
A kind of application of Pu-Er ripe tea extract in blood-pressure drug or food is prepared Download PDFInfo
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Abstract
The present invention provides a kind of Pu-Er ripe tea extract, preparation method is:Take Pu'er cooked tea, add water to cook, tea grounds discards, extracting solution concentration after plus ethyl alcohol stand, precipitation and separation, supernatant drying to get.And application of the Pu-Er ripe tea extract in blood-pressure drug or health food is prepared.
Description
Technical field
The present invention relates to pharmacy and field of health care food, specially a kind of Pu-Er ripe tea extract and preparation method thereof and its
Application in treatment blood-pressure drug or health food is prepared.
Background technology
With its long history, the charm of rich culture and glycol is world known yunnan puer tea.Pu'er tea originates in
Ground is mainly in the Simao Diqu in Yunnan and Xishuangbanna.With the popularization of large leaf, Sichuan, Guangdong, Guangxi also become Pu'er tea
The province of production.In recent years, with the development of economy, the health care consciousness enhancing of people, health, health care become ordinary people
The theme of daily life, Pu'er tea have attracted the extensive pass of various circles of society, especially each side scholar with its unique health-care efficacy
Note, civil is even more to have risen one upsurge for buying Pu'er, hiding Pu'er, drinking Pu'er therewith.
By processing technology and qualitative characteristics, Pu'er tea is divided into two kinds of Pu'er tea life tea and puerh tea.Raw tea is by great Ye
Kind sun withering tea is made through techniques such as tidewater, steam pressure moldings, and Appearance color is blackish green, greenish-yellow limpid, the dense time sweet, tea residue of flavour of soup look
It is plump yellowish green.Ripe tea needs the zymotechnique after in process, Appearance color is reddish brown, red dense bright, the flavour alcohol of soup look and
Return it is sweet, tea residue is reddish brown.Ripe tea is in rear fermentation process, under the action of enzymatic or non-enzymatic, polyphenols, amino acid, can
Dissolubility sugar and water extraction content change, and polyphenols is gradually oxidized to theaflavin, thearubigin and theabrownin, becomes
The basic reason of raw, the ripe tea quality generation difference in Pu'er.
After Pu'er cooked tea is fermented, under the action of enzyme, and many new nutriments are produced, therefore in common tea
On the basis of, and have more effects, such as:Lipid-loweringing, weight-reducing, blood pressure lowering, anti arteriosclerosis, anti-cancer, anticancer, nourishing the stomach and protecting stomach, healthy tooth
Protect tooth, anti-inflammatory, sterilization, anti-aging etc..
The lipid-reducing function of Pu'er cooked tea has received wide acceptance at present.But antihypertensive effect never directly experiment
Data are supported.Even there is relevant report within 2007, negate the antihypertensive effect of Pu'er cooked tea.
It is drunk since current consumption habit is only brewed with tealeaves, when functional component is brewed number, soaking temperature, brewed
Between wait influence and cause dissolution difference, it is impossible to be fully utilized.Therefore, effective component extracting becomes current research from Pu'er tea
Hot spot.
The method of effective component extracting has much from Pu'er tea, according to different extracting methods, can extract to obtain general
Different active ingredients in Pu'er tea tea, such as Chinese patent CN200510010871.2, patent CN200910069867.1, patent
CN200910069869.0, patent CN200910069879.4, patent CN200910069873.7, patent
CN200910228699.6 etc., these methods be substantially all employ water carry, filter, concentrating,(Alcohol precipitation,)Drying and other steps,
But all there is differences again between each step and extracting parameter, these are different, directly result in the difference of the product finally obtained.
The present invention is obtains target product, on the basis of prior art Pu'er tea extracting method, by experiment repeatedly
Research, and it is made that some improvement, finally obtain the Pu-Er ripe tea extract and preparation method thereof of the present invention, and real with animal
Verification understands that the Pu-Er ripe tea extract has the pharmacological action of decompression.
Invention content
It is an object of the present invention to provide a kind of Pu-Er ripe tea extracts and preparation method thereof.
It is another object of the present invention to provide pharmaceutical composition containing extract of the present invention and preparation method thereof with
And its purposes in the drug or health food for preparing blood pressure lowering.
The Pu-Er ripe tea extract of the present invention is prepared as follows to obtain:
Pu'er cooked tea is taken to add water to cook, tea grounds discards, and after extracting solution concentration plus ethyl alcohol is stood, and precipitation and separation, supernatant is done
It is dry to get.
Preferably,
The Pu-Er ripe tea extract of the present invention is prepared as follows to obtain:
It takes Pu'er cooked tea appropriate, adds in 5-20 times and measure water heating and refluxing extraction 1-3 times, each 0.5-3 hours, filtering, tea
Slag adds 4-16 times to measure water, and heating and refluxing extraction 0.5-3 hours filters, and tea grounds discards, and merges extracting solution, filtering, filtrate 50-80
DEG C reduced vacuum is concentrated into relative density 1.02-1.15, centrifuges, obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 50-
80%, it stands overnight, centrifuges, obtain alcohol precipitation precipitation, heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant, drying is
.
It is furthermore preferred that
The Pu-Er ripe tea extract of the present invention is prepared as follows to obtain:
It takes Pu'er cooked tea appropriate, adds in 6-12 times and measure 50-100 DEG C of water heating and refluxing extraction 1-3 times, it is 0.5-3 hours each,
Filtering, tea grounds add 5-10 times to measure water, and 50-100 DEG C of heating and refluxing extraction 0.5-3 hour, filtering, tea grounds discards, merging extracting solution,
Filtering, 50-80 DEG C of reduced vacuum of filtrate are concentrated into relative density 1.02-1.15, centrifuge, obtain centrifuged supernatant, add 95% ethyl alcohol alcohol
Alcohol content 50-80% is sink to, is stood overnight, is centrifuged, obtains alcohol precipitation precipitation, heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains
Clear liquid, spray drying or reduced vacuum drying to get.
Most preferably,
The Pu-Er ripe tea extract of the present invention is prepared as follows to obtain:
It takes Pu'er cooked tea appropriate, adds 10 times of amount 80 DEG C of water heating and refluxing extractions 2 times, 1 hour every time, filtering, tea grounds added 8 times
Measure water, 80 DEG C of heating and refluxing extractions 0.5 hour, filtering, tea grounds discards, merge extracting solution, cross 300 mesh filter clothes, 70 DEG C of filtrate subtracts
Pressure is concentrated in vacuo to relative density 1.05-1.10, and tubular type centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 80%,
It stands overnight, centrifuges, obtain alcohol precipitation precipitation, heating water is redissolved to proportion 1.05-1.10, and tubular type centrifugation obtains supernatant, is spray-dried
Or 70 DEG C of reduced vacuum dryings to get.
It, can be as the activity with buck functionality through Pu-Er ripe tea extract of the invention made from method made above
Ingredient is prepared into pharmaceutical composition or health food.Health food of the present invention includes but not limited to:Beverage, dairy products,
Tealeaves, cake etc..
The present invention pharmaceutical composition in active constituents of medicine, in the composition shared weight percent can be
0.1-99.9%, remaining is pharmaceutically acceptable carrier.The pharmaceutical composition of the present invention, exists, the list in a unit
Position dosage form refers to the unit of preparation, such as every of tablet, every capsule of capsule, every bottle of oral liquid, granule it is every
Bag etc..
The pharmaceutical composition of the present invention can be any pharmaceutical dosage form, these dosage forms include:Tablet, sugar coated tablet,
Film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, mouth containing agent, granule, electuary, ball
Agent, powder, paste, sublimed preparation, suspension, pulvis, solution, injection, suppository, ointment, emplastrum, creme, spray, drop
Agent, patch.The preparation of the present invention, preferably peroral dosage form, such as:Capsule, tablet, oral liquid, granule, pill, powder,
Sublimed preparation, paste etc..
The pharmaceutical composition of the present invention, the preparation of oral medication can contain common excipient, such as adhesive, filling
Agent, diluent, tablet agent, lubricant, disintegrant, colorant, flavoring agent and wetting agent when necessary can be coated tablet.
Applicable filler includes cellulose, mannitol, lactose and other similar fillers.Suitable disintegrant packet
Include starch, polyvinylpyrrolidone and starch derivatives, such as sodium starch glycollate.Suitable lubricant includes, such as firmly
Fatty acid magnesium.Suitable pharmaceutically acceptable wetting agent includes lauryl sodium sulfate.
Can solid oral composition be prepared by common methods such as mixing, filling, tablettings.Work can be made by carrying out mixing repeatedly
Property substance be distributed in entirely using a large amount of fillers those compositions in.
The form of oral liquid for example can be aqueous or oily suspensions, solution, emulsion, syrup or elixir,
Or can be a kind of dry products that water or other suitable carriers can be used to compound before use.This liquid preparation can contain
Conventional additive, such as suspending agent, such as sorbierite, syrup, methylcellulose, gelatin, hydroxyethyl cellulose, carboxymethyl are fine
Dimension element, aluminium stearate gel or hydrogenated edible fats, emulsifier, such as lecithin, anhydro sorbitol monooleate or Arab
Glue;Non-aqueous carrier(They can include edible oil), such as the oily ester of the ester of apricot kernel oil, fractionated coconut oil, such as glycerine,
Propylene glycol or ethyl alcohol;Preservative, such as para hydroxybenzene methyl esters or propylparaben or sorbic acid, and if desired,
Contain conventional flavouring agent or colorant.
For injection, the fluid unit dosage form of preparation contains the active material and sterile carrier of the present invention.According to carrier
And concentration, this compound can be suspended or be dissolved.The preparation of solution is typically by the way that active material is dissolved in a kind of load
In body, disinfection is filtered before a kind of suitable bottle or ampoule is loaded into, is then sealed.For example a kind of local anaesthesia of auxiliary material
Agent, preservative and buffer can also be dissolved in this carrier.It, can be after bottle be packed by this in order to improve its stability
Kind composition frost, and under vacuum remove water.
The pharmaceutical composition of the present invention, suitable pharmaceutically acceptable load is optionally added in when being prepared into medicament
Body, the pharmaceutically acceptable carrier are selected from:Mannitol, sorbierite, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, salt
Sour cysteine, thioacetic acid, methionine, injection Vitamin B_6 DTA disodiums, Ethylenediaminetetraacetic Acid Calcium Salt, carbonate, the acetic acid of monovalence alkali metal
Salt, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, wheat
Bud sugar, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its
Derivative, alginates, gelatin, polyvinylpyrrolidone, glycerine, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surface-active
Agent, polyethylene glycol, cyclodextrin, beta-cyclodextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate etc..
The composition of the present invention determines usage and dosage according to the situation of patient when in use, can often taken three times per day, each 1-
20 doses, such as:1-20 bags or grain or piece.
The invention also includes the application of Pu-Er ripe tea extract of the invention in blood-pressure drug or health food is prepared.
The Pu-Er ripe tea extract of the present invention has the function of good health care and treatment, particularly in terms of blood pressure lowering.
Below by way of spontaneous hypertension rat(SHR)Pharmacological experimental data, to illustrate beneficial effects of the present invention:
1 material and method
1.1 experiment material
(1)Tested material:Pu-Er ripe tea extract is provided by Tasly Institute's Chinese medicine, lot number:20110608.
(2)Other reagents and key instrument:Positive drug uses Irbesartan(Irbesartan Tablets, France's production, Hangzhou match promise
Fei Anwante people's livelihood pharmaceutical Co. Ltd dispenses.Lot number of the repackaged products:0906193, batch number:1797, the date of manufacture:
2009.02).The non-invasive blood pressure instrument of Kent companies of the U.S.(CODATM2).
(3)Experimental animal:Using spontaneous hypertension rat(SHR), it is limited purchased from Beijing dimension tonneau China experimental animal technology
Company, quality certification number:SCXK (capital) 2006-0009.Rearing conditions:It is raised in air-conditioned animal housing, temperature 20-25OC, phase
To humidity 40%-60%, per 5, cage, feed is added at regular time and quantity, eats rat special feed, and free water is daily to replace pad
Material.
1.2 experimental method
(1)Grouping:Totally 3 groups:Model group, positive drug Irbesartan(15.5mg/kg)Group, Pu-Er ripe tea extract(1.0g/
kg)Group.Each group is SHR rats, every group 10.
(2)Experimental program:After animal reaches laboratory, pre- raising one week measures basic blood pressure(Basic blood pressure needs to measure
1-2 weeks, it is ensured that animal is already adapted to instrument, and the blood pressure measured is true value).It is grouped at random according to basic blood pressure.After grouping, next day
Start to test, each administration group presses 1ml/100g gastric infusions, successive administration one month.It is primary that period measures weekly blood pressure.And it slaps
It has held the time, has ensured that animal is measuring at the same time substantially, avoid the influence of itself fluctuation of blood pressure in the daytime.
(3)Observation index:Blood pressure, weight, heart rate.
2 experimental results
(1)Tested material surrounding is given to each group animal mean arterial pressure(MBP)Influence
According to experimental result, successive administration one month, the mean arterial pressure of positive drug group is by administration second week and mould
Type group compares to be reduced with conspicuousness(p<0.05), it is continued until administration 4th week.Third is administered in Pu-Er ripe tea extract group
All 4th week mean arterial pressures and model group compare to be reduced with conspicuousness(p<0.05).It the results are shown in Table 1.
Table 1 gives influence mean ± SD of the tested material surrounding to each group animal mean arterial pressure
Note:Model group in the same time compares:*:p<0.05;**:p<0.01;***:p<0.001
(2)Tested material surrounding is given to each group animal systolic pressure(SBP)Influence
According to experimental result, continuously give surrounding, the systolic pressure of positive drug group by administration second week with model group ratio
Relatively there is conspicuousness to reduce(p<0.05), it is continued until administration 4th week.Each tested material group is in administration 4th week systolic pressure
Comparing with model group reduces with conspicuousness(p<0.05).It the results are shown in Table 2.
Table 2 gives influence mean ± SD of the tested material surrounding to each group animal systolic pressure
Note:Model group in the same time compares:*:p<0.05;**:p<0.01;***:p<0.001
(3)Tested material surrounding is given to each group animal diastolic pressure(DBP)Influence
Continuous to give surrounding, the diastolic pressure of positive drug group being administered first week with model group by comparing with conspicuousness drop
It is low(p<0.05), it is continued until administration 4th week.Pu-Er ripe tea extract group administration third week and 4th week diastolic pressure and
Model group compares to be reduced with conspicuousness(p<0.05).It the results are shown in Table 3.
Table 3 gives influence mean ± SD of the tested material surrounding to each group animal diastolic pressure
Note:Model group in the same time compares:*:p<0.05;**:p<0.01;***:p<0.001
(4)Give influence of the tested material surrounding to each group animal heart rate
The heart rate of each group animal is shown no obvious abnormalities within normal range (NR).It the results are shown in Table 4.
Table 4 gives influence mean ± SD of the tested material surrounding to each group animal heart rate
Note:Model group in the same time compares:*:p<0.05;**:p<0.01;***:p<0.001
(5)Give influence of the tested material surrounding to each group the weight of animals
The weight and model group of each group animal are relatively without significant changes, also no abnormal trend.Each group the weight of animals
Growth curve becomes in rising.It the results are shown in Table 5.
Table 5 gives influence mean ± SD of the tested material surrounding to each group the weight of animals
Note:Model group in the same time compares:*:p<0.05;**:p<0.01;***:p<0.001
3 conclusions
In this experimental result, Pu-Er ripe tea extract group shows significant antihypertensive active, has clear and definite
The effect for reducing spontaneous hypertension rat mean arterial pressure, systolic pressure and diastolic pressure.
Specific embodiment:
It is further illustrated the present invention below by specific embodiment, following embodiments are for illustrating rather than
Limitation of the present invention, the simple modifications that essence according to the present invention carries out the present invention belong to claimed model
It encloses.
Embodiment 1
Pu'er cooked tea 1kg is taken, adds 10 times of amount 80 DEG C of water heating and refluxing extractions 2 times, 1 hour every time, filtering, tea grounds added 8 times
Measure water, 80 DEG C of heating and refluxing extractions 0.5 hour, filtering, tea grounds discards, merge extracting solution, cross 300 mesh filter clothes, 70 DEG C of filtrate subtracts
Pressure is concentrated in vacuo to relative density 1.05-1.10, and tubular type centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 80%,
It stands overnight, centrifuges, obtain alcohol precipitation precipitation, heating water is redissolved to proportion 1.05-1.10, tubular type centrifugation, obtains supernatant, spraying is dry
It is dry, obtain Pu-Er ripe tea extract.
Embodiment 2
Pu'er cooked tea 1kg is taken, adds in 5 times of amount 100 DEG C of water heating and refluxing extractions 2 times, 1 hour every time, filtering, tea grounds added 5
Times amount water, 100 DEG C of heating and refluxing extractions 0.5 hour, filtering, tea grounds discard, and merge extracting solution, filtering, and 70 DEG C of decompressions of filtrate are true
Sky is concentrated into relative density 1.02-1.15, and centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 75%, was stood
Night, centrifugation obtain alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, centrifugation, obtain supernatant, and 70 DEG C of reduced vacuums are dried,
Obtain Pu-Er ripe tea extract.
Embodiment 3
Pu'er cooked tea 1kg is taken, adds in 12 times of amount 80 DEG C of water heating and refluxing extractions 2 times, 0.5 hour every time, filtering, tea grounds added
10 times of amount water, 80 DEG C of heating and refluxing extractions 0.5 hour, filtering, tea grounds discard, and merge extracting solution, filtering, and 50 DEG C of decompressions of filtrate are true
Sky is concentrated into relative density 1.02-1.15, and centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 70%, was stood
Night, centrifugation obtain alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant, is spray-dried, it is ripe to obtain Pu'er
Tea extraction.
Embodiment 4
Pu'er cooked tea 10kg is taken, adds in 8 times of amount 50 DEG C of water heating and refluxing extractions 2 times, 3 hours every time, filtering, tea grounds added 8
Times amount water, 50 DEG C of heating and refluxing extractions 1 hour, filtering, tea grounds discard, and merge extracting solution, filtering, and 60 DEG C of reduced vacuums of filtrate are dense
Relative density 1.02-1.15 is reduced to, is centrifuged, is obtained centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 60%, is stood overnight, from
The heart obtains alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant, is spray-dried, and obtains Pu'er cooked tea extraction
Object 1.1kg.
Embodiment 5
Pu'er cooked tea 1kg is taken, 20 times of amount 100 DEG C of water heating and refluxing extractions 3 times is added in, 0.5 hour every time, filters, tea grounds
Add 16 times of amount water, 100 DEG C of heating and refluxing extractions 0.5 hour, filtering, tea grounds discards, and merges extracting solution, filtering, 80 DEG C of filtrate subtracts
Pressure is concentrated in vacuo to relative density 1.02-1.15, centrifuges, obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 80%, stands
Overnight, centrifugation obtains alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant, 70 DEG C of reduced vacuums are done
It is dry, obtain Pu-Er ripe tea extract 130g.
Embodiment 6
Pu'er cooked tea 1kg is taken, adds in 16 times of amount 80 DEG C of water heating and refluxing extractions 1 time, 2 hours every time, filtering, tea grounds added 16
Times amount water, 80 DEG C of heating and refluxing extractions 2 hours, filtering, tea grounds discard, and merge extracting solution, filtering, and 50 DEG C of reduced vacuums of filtrate are dense
Relative density 1.02-1.15 is reduced to, is centrifuged, is obtained centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 50%, is stood overnight, from
The heart obtains alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant, is spray-dried, and obtains Pu'er cooked tea extraction
Object 120g.
Embodiment 7
According to any one extract in embodiment 1-6, customary adjuvant is added in, using the conventional method on galenic pharmacy, system
Piece agent.
Embodiment 8
According to any one extract in embodiment 1-6, customary adjuvant is added in, using the conventional method on galenic pharmacy, system
Into capsule.
Embodiment 9
According to any one extract in embodiment 1-6, customary adjuvant is added in, using the conventional method on galenic pharmacy, system
Into granule.
Embodiment 10
According to any one extract in embodiment 1-6, customary adjuvant is added in, using the conventional method on galenic pharmacy, system
Into powder.
Embodiment 11
According to any one extract in embodiment 1-6, customary adjuvant is added in, using the conventional method on galenic pharmacy, system
Into pill.
Embodiment 12
According to any one extract in embodiment 1-6, customary adjuvant is added in, using the conventional method on galenic pharmacy, system
Into suppository.
Embodiment 13
It according to any one extract in embodiment 1-6, is added in tea product, made by a certain percentage with common process
Into the health protection tea with decompression.
Embodiment 14
According to any one extract in embodiment 1-6, it is added in dairy products, is made by a certain percentage with common process
Health food with buck functionality.
Embodiment 15
Any one extract in embodiment 1-6, with common process is added in beverage, is made by a certain percentage
Health drink with buck functionality.
Embodiment 16
Any one extract in embodiment 1-6, with common process is added in cake, is made by a certain percentage
Health care cake with buck functionality.
Claims (8)
1. application of a kind of Pu-Er ripe tea extract in blood-pressure drug or health food is prepared, which is characterized in that Pu'er is ripe
Tea extraction is prepared as follows to obtain by Pu'er cooked tea:Pu'er cooked tea is taken, is added water to cook, tea grounds discards, and extracting solution is dense
After contracting plus ethyl alcohol stand, precipitation and separation, supernatant drying to get.
2. the application of Pu-Er ripe tea extract as described in claim 1, which is characterized in that Pu-Er ripe tea extract is according to such as
Lower section method is prepared:It takes Pu'er cooked tea appropriate, adds in 5-20 times and measure water heating and refluxing extraction 1-3 times, each 0.5-3 is small
When, filtering, tea grounds adds 4-16 times to measure water, and heating and refluxing extraction 0.5-3 hours filters, and tea grounds discards, and merges extracting solution, filters,
50-80 DEG C of reduced vacuum of filtrate is concentrated into relative density 1.02-1.15, and centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to
Alcohol content 50-80%, stands overnight, centrifugation, obtains alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant
Liquid is drying to obtain.
3. the application of Pu-Er ripe tea extract as described in claim 1, which is characterized in that Pu-Er ripe tea extract is according to such as
Lower section method is prepared:It takes Pu'er cooked tea appropriate, adds in 6-12 times and measure 50-100 DEG C of water heating and refluxing extraction 1-3 times, every time
0.5-3 hours, filtering, tea grounds added 5-10 times to measure water, and 50-100 DEG C of heating and refluxing extraction 0.5-3 hour filters, and tea grounds discards,
Merging extracting solution, filtering, 50-80 DEG C of reduced vacuum of filtrate is concentrated into relative density 1.02-1.15, centrifuges, obtains centrifuged supernatant,
95% ethyl alcohol alcohol is added to be sink to alcohol content 50-80%, is stood overnight, is centrifuged, obtains alcohol precipitation precipitation, heating water is redissolved to proportion 1.02-
1.15, centrifugation obtains supernatant, spray drying or reduced vacuum drying to get.
4. the application of Pu-Er ripe tea extract as described in claim 1, which is characterized in that Pu-Er ripe tea extract is according to such as
Lower section method is prepared:It takes Pu'er cooked tea appropriate, adds 10 times of amount 80 DEG C of water heating and refluxing extractions 2 times, 1 hour every time, filter,
Tea grounds adds 8 times of amount water, 80 DEG C of heating and refluxing extractions 0.5 hour, filtering, and tea grounds discards, merges extracting solution, cross 300 mesh filter clothes, filter
70 DEG C of reduced vacuums of liquid are concentrated into relative density 1.05-1.10, and tubular type centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to
Alcohol content 80%, stands overnight, centrifugation, obtains alcohol precipitation precipitation, and heating water is redissolved to proportion 1.05-1.10, and tubular type centrifugation obtains supernatant
Liquid, spray drying or 70 DEG C of reduced vacuum dryings to get.
5. application according to claim 1, which is characterized in that the drug is any pharmaceutical dosage form.
6. application according to claim 5, which is characterized in that the pharmaceutical dosage form is selected from:Tablet, capsule, oral liquid, mouth containing
Agent, granule, pill, powder, paste, sublimed preparation, suspension, pulvis, solution, suppository, creme, spray, drops or patch.
7. application according to claim 1, which is characterized in that the health food is selected from:Beverage, dairy products, tealeaves or cake.
8. the preparation method of Pu-Er ripe tea extract as described in claim 1, which is characterized in that step is as follows:Take Pu'er ripe
Appropriate tea adds in 5-20 times and measures water heating and refluxing extraction 1-3 times, each 0.5-3 hours, and filtering, tea grounds adds 4-16 times to measure water, adds
Circumfluence distillation 0.5-3 hours, filtering, tea grounds discard, and merge extracting solution, filtering, and 50-80 DEG C of reduced vacuum of filtrate is concentrated into phase
To density 1.02-1.15, centrifugation obtains centrifuged supernatant, 95% ethyl alcohol alcohol is added to be sink to alcohol content 50-80%, is stood overnight, from
The heart obtains alcohol precipitation precipitation, and heating water is redissolved to proportion 1.02-1.15, and centrifugation obtains supernatant, is drying to obtain.
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