CN103505564A - Application of Pu-Er ripe tea extract in preparing blood pressure lowering medicine or food - Google Patents
Application of Pu-Er ripe tea extract in preparing blood pressure lowering medicine or food Download PDFInfo
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Abstract
The invention provides a Pu-Er ripe tea extract, which is prepared by the steps of decorating Pu-Er ripe tea in water, discarding tea residue, condensing an extracting solution, adding ethanol and standing by, separating and precipitating, and drying supernatant liquid to obtain the Pu-Er ripe tea extract. Meanwhile, the invention discloses application of the Pu-Er ripe tea extract in preparing blood pressure lowering medicine or healthcare food.
Description
Technical field
The present invention relates to pharmacy and field of health care food, be specially a kind of Folium camelliae assamicae (processed) extract and preparation method thereof and its application in preparation treatment Altace Ramipril or health food.
Background technology
Yunnan puer tea is with its long history, and the charm of rich culture and glycol is world known.Folium camelliae assamicae original producton location is mainly in Simao Diqu and Xishuangbanna in Yunnan.Along with the popularization of large leaf, Sichuan, Guangdong, Guangxi also become the province that Folium camelliae assamicae is produced.In the last few years, along with expanding economy, people's health care consciousness strengthens, health preserving, health care become the theme of ordinary people daily life, Folium camelliae assamicae is with its unique health-care effect, attracted various circles of society, the scholar's of each side extensive concern especially, has among the peoplely risen especially one thereupon and has bought the upsurge in ,Yin Pu'er, ,Zang Pu'er, Pu'er.
By processing technique and qualitative characteristics, Folium camelliae assamicae is divided into two kinds of the raw tea of Folium camelliae assamicae and the ripe tea of Folium camelliae assamicae.Raw tea shines Folium Camelliae sinensis by large leaf to be made through techniques such as tidewater, steam pressure molding, and Appearance color is blackish green, dense time of greenish-yellow limpid, the flavour of soup color is sweet, leaf base fertilizer is thick yellowish green.Ripe tea needs through after fermentation technique in the course of processing, its Appearance color is reddish brown, red dense bright, the flavour alcohol of soup color and return sweet, Radix Phyllagathis fordii is brown.Ripe tea is in after fermentation process, under the effect of enzymatic or non-enzymatic, polyphenols, aminoacid, soluble sugar and water extraction content change, and polyphenols is oxidized to theaflavin, thearubigins and abrownin gradually, become the basic reason that raw, the ripe tea quality in Pu'er produces difference.
Folium camelliae assamicae (processed) is after fermentation, under the effect of enzyme, many new nutrient substance have been produced again, therefore on the basis of common tea, there have been again more effects, as stomach, healthy tooth tooth protection, antiinflammatory, sterilization, anti-ageing waiting for a long time are protected in: blood fat reducing, fat-reducing, blood pressure lowering, arteriosclerosis, anti-cancer, anticancer, nourishing the stomach.
The lipid-reducing function of Folium camelliae assamicae (processed) receives wide acceptance at present.But hypotensive effect never has direct experimental data support.Within 2007, even having relevant report, negates the hypotensive effect of Folium camelliae assamicae (processed).
Because current consumption habit just brews and drinks with Folium Camelliae sinensis, functional component is brewed number of times, brews temperature, brewing time etc. affects and causes stripping difference, can not utilize completely.Therefore, from Folium camelliae assamicae, effective component extracting becomes current study hotspot.
From Folium camelliae assamicae, the method for effective component extracting has a lot, according to different extracting method, can extract the different effective ingredient that obtain in Folium camelliae assamicae, Chinese patent CN200510010871.2 for example, patent CN200910069867.1, patent CN200910069869.0, patent CN200910069879.4, patent CN200910069873.7, patent CN200910228699.6 etc., these methods have all adopted water extraction substantially, filter, concentrated, (precipitate with ethanol, ) drying and other steps, but between each step and extracting parameter, all exist again difference, these differences, the difference that directly causes the product that finally obtains.
The present invention is for obtaining target product, on the basis of prior art Folium camelliae assamicae extracting method, through experimental study repeatedly, some improvement have been made again, finally drawn Folium camelliae assamicae (processed) extract of the present invention and preparation method thereof, and proved that with zoopery this Folium camelliae assamicae (processed) extract has the pharmacological action of blood pressure lowering.
Summary of the invention
One object of the present invention is to provide a kind of Folium camelliae assamicae (processed) extract and preparation method thereof.
Another object of the present invention be to provide pharmaceutical composition of containing extract of the present invention and preparation method thereof with and preparing the medicine of blood pressure lowering or the purposes in health food.
Folium camelliae assamicae (processed) extract of the present invention is prepared as follows and obtains:
Get Folium camelliae assamicae (processed) and decoct with water, tea grounds discards, and adds ethanol standing after extracting solution is concentrated, precipitation separation, and supernatant is dry, obtains.
Preferably,
Folium camelliae assamicae (processed) extract of the present invention is prepared as follows and obtains:
Get Folium camelliae assamicae (processed) appropriate, add 5-20 times of water gaging heating and refluxing extraction 1-3 time, each 0.5-3 hour, filters, tea grounds adds 4-16 times of water gaging, and heating and refluxing extraction 0.5-3 hour filters, and tea grounds discards, merge extractive liquid,, filters, and a filtrate 50-80 ℃ reduced vacuum is concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 50-80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, be drying to obtain.
It is preferred,
Folium camelliae assamicae (processed) extract of the present invention is prepared as follows and obtains:
Get Folium camelliae assamicae (processed) appropriate, add 6-12 times of water gaging 50-100 ℃ heating and refluxing extraction 1-3 time, each 0.5-3 hour, filter, tea grounds adds 5-10 times of water gaging, 50-100 ℃ of heating and refluxing extraction 0.5-3 hour, filter, tea grounds discards, merge extractive liquid, filter, a filtrate 50-80 ℃ reduced vacuum is concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, adding 95% ethanol alcohol is sink to containing alcohol amount 50-80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, spraying is dry or reduced vacuum is dry, obtain.
It is most preferred,
Folium camelliae assamicae (processed) extract of the present invention is prepared as follows and obtains:
Get Folium camelliae assamicae (processed) appropriate, add 80 ℃ of heating and refluxing extraction of 10 times of water gagings 2 times, each 1 hour, filter, tea grounds adds 8 times of water gagings, 80 ℃ of heating and refluxing extraction 0.5 hour, filter, tea grounds discards, and merge extractive liquid, is crossed 300 order filter clothes, 70 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.05-1.10, tubular type is centrifugal, obtains centrifuged supernatant, adds 95% ethanol alcohol and is sink to containing alcohol amount 80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.05-1.10, tubular type is centrifugal, obtain supernatant, spraying is dry or 70 ℃ of reduced vacuum are dry, obtains.
The Folium camelliae assamicae (processed) extract of the present invention making through above preparation method, can be used as the active fraction preparation with buck functionality and becomes pharmaceutical composition or health food.Health food of the present invention includes but not limited to: beverage, milk product, Folium Camelliae sinensis, cake etc.
Active constituents of medicine in pharmaceutical composition of the present invention, its shared percentage by weight in compositions can be 0.1-99.9%, all the other are medicine acceptable carrier.Pharmaceutical composition of the present invention, exists with unit dosage form, and described unit dosage form refers to the unit of preparation, as every of tablet, and every capsules of capsule, every bottle of oral liquid, every bag of granule etc.
Pharmaceutical composition of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, peroral dosage form preferably, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc.
Pharmaceutical composition of the present invention, the preparation of its oral administration can contain conventional excipient, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet if desired.
Applicable filler comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of suitable medicine comprises sodium lauryl sulphate.
Can fill by mixing, the method that tabletting etc. are conventional is prepared solid oral composition.Repeatedly mix and can make active substance be distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid can be for example aqueous or oily suspensions, solution, Emulsion, syrup or elixir, or can be a kind of available water before use or the composite dry products of other suitable carrier.This liquid preparation can contain conventional additive, such as suspending agent, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat, emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if need, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this compound can be suspended or dissolve.The preparation of solution is normally by active substance being dissolved in a kind of carrier, and filter-sterilized before being packed into a kind of suitable bottle or ampoule, then seals.Adjuvant for example a kind of local anesthetic, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after packing bottle into, this compositions is freezing, and under vacuum, water is removed.
Pharmaceutical composition of the present invention, when being prepared into medicament, optionally add applicable medicine acceptable carrier, described medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Compositions of the present invention is determined usage and dosage according to patient's situation in use, can take every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet.
The present invention also comprises the application of Folium camelliae assamicae (processed) extract of the present invention in preparing Altace Ramipril or health food.
Folium camelliae assamicae (processed) extract of the present invention has good health care and treatment function, particularly aspect blood pressure lowering.
Below, by spontaneous hypertension rat (SHR) pharmacological experimental data, beneficial effect of the present invention is described:
1 materials and methods
1.1 experiment material
(1) tested material: Folium camelliae assamicae (processed) extract is provided by Tasly Institute's Chinese medicine, lot number: 20110608.
(2) other reagent and key instrument: positive drug adopts irbesartan, and (Irbesartan Tablets, France produces, the packing of Hangzhou Sanofi-Aventis people's livelihood pharmaceutical Co. Ltd.Lot number of the repackaged products: 0906193, batch number: 1797, the date of manufacture: 2009.02).Non-invasive blood pressure instrument (the CODA of U.S. Kent company
tM2).
(3) laboratory animal: adopt spontaneous hypertension rat (SHR), purchased from Beijing Vital River Experimental Animals Technology Co., Ltd., the quality certification number: SCXK (capital) 2006-0009.Raising condition: in air-conditioned animal housing, raise, temperature 20-25OC, relative humidity 40%-60%, 5, every cage, adds feedstuff at regular time and quantity, and edible rat special feed, freely drinks water, and changes bedding and padding every day.
1.2 experimental technique
(1) grouping: totally 3 groups: model group, positive drug irbesartan (15.5mg/kg) group, Folium camelliae assamicae (processed) extract (1.0g/kg) group.Each group is SHR rat, 10 every group.
(2) experimental program: animal arrives after laboratory, raises one week in advance, measures basic blood pressure (basic blood pressure need to be measured 1-2 week, guarantees that animal has adapted to instrument, and the blood pressure measuring is true value).According to basic blood pressure random packet.After grouping, start experiment next day, each administration group is pressed 1ml/100g gastric infusion, successive administration one month.Measure weekly blood pressure once during this time.And grasp the time, guarantee that animal measuring substantially at the same time, avoid in the daytime the impact of self fluctuation of blood pressure.
(3) observation index: blood pressure, body weight, heart rate.
2 experimental results
(1) give the impact of tested material surrounding on each treated animal mean arterial pressure (MBP)
According to experimental result, successive administration one month, the mean arterial pressure of positive drug group starts relatively have significance with model group by administration second week and reduces (p < 0.05), is continued until administration 4th week.The 3rd week 4th week mean arterial pressure of Folium camelliae assamicae (processed) extract group administration and model group relatively have significance and reduce (p < 0.05).The results are shown in Table 1.
Table 1 gives the affect mean ± SD of tested material surrounding on each treated animal mean arterial pressure
Note: and model group comparison in the same time:<sup TranNum="117">*</sup>: p<0.05;<sup TranNum="118">*</sup>: p<0.01;<sup TranNum="119">* *</sup>: p<0.001
(2) give the impact of tested material surrounding on each treated animal systolic pressure (SBP)
According to experimental result, give continuously surrounding, the systolic pressure of positive drug group starts relatively have significance with model group by administration second week and reduces (p < 0.05), is continued until administration 4th week.Each tested material group all relatively has significance reduction (p < 0.05) at administration 4th week systolic pressure and model group.The results are shown in Table 2.
Table 2 gives the affect mean ± SD of tested material surrounding on each treated animal systolic pressure
Note: and model group comparison in the same time:<sup TranNum="126">*</sup>: p<0.05;<sup TranNum="127">*</sup>: p<0.01;<sup TranNum="128">* *</sup>: p<0.001
(3) give the impact of tested material surrounding on each treated animal diastolic pressure (DBP)
Give continuously surrounding, the diastolic pressure of positive drug group within first week, is started by administration and model group relatively has significance reduction (p < 0.05), is continued until administration 4th week.Folium camelliae assamicae (processed) extract group relatively has significance reduction (p < 0.05) with 4th week diastolic pressure and model group on the 3rd week in administration.The results are shown in Table 3.
Table 3 gives the affect mean ± SD of tested material surrounding on each treated animal diastolic pressure
Note: and model group comparison in the same time:<sup TranNum="137">*</sup>: p<0.05;<sup TranNum="138">*</sup>: p<0.01;<sup TranNum="139">* *</sup>: p<0.001
(4) give the impact of tested material surrounding on each treated animal heart rate
The heart rate of each treated animal all, within normal range, shows no obvious abnormalities.The results are shown in Table 4.
Table 4 gives the affect mean ± SD of tested material surrounding on each treated animal heart rate
Note: and model group comparison in the same time:<sup TranNum="146">*</sup>: p<0.05;<sup TranNum="147">*</sup>: p<0.01;<sup TranNum="148">* *</sup>: p<0.001
(5) give the impact of tested material surrounding on each treated animal body weight
The body weight of each treated animal and model group relatively do not have significant change, no abnormal trend yet.Each treated animal body weight gain curve is all rising and becomes.The results are shown in Table 5.
Table 5 gives the affect mean ± SD of tested material surrounding on each treated animal body weight
Note: and model group comparison in the same time:<sup TranNum="155">*</sup>: p<0.05;<sup TranNum="156">*</sup>: p<0.01;<sup TranNum="157">* *</sup>: p<0.001
3 conclusions
In this experimental result, Folium camelliae assamicae (processed) extract group has shown significant antihypertensive active, has the effect of clear and definite reduction spontaneous hypertension rat mean arterial pressure, systolic pressure and diastolic pressure.
The specific embodiment:
Below by specific embodiment, further illustrate the present invention, following embodiment is that the simple modifications that essence according to the present invention is carried out the present invention all belongs to the scope of protection of present invention for the present invention rather than limitation of the present invention are described.
Embodiment 1
Get Folium camelliae assamicae (processed) 1kg, add 80 ℃ of heating and refluxing extraction of 10 times of water gagings 2 times, each 1 hour, filter, tea grounds adds 8 times of water gagings, 80 ℃ of heating and refluxing extraction 0.5 hour, filter, tea grounds discards, and merge extractive liquid, is crossed 300 order filter clothes, 70 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.05-1.10, tubular type is centrifugal, obtains centrifuged supernatant, adds 95% ethanol alcohol and is sink to containing alcohol amount 80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.05-1.10, tubular type is centrifugal, obtain supernatant, spraying is dry, obtains Folium camelliae assamicae (processed) extract.
Embodiment 2
Get Folium camelliae assamicae (processed) 1kg, add 100 ℃ of heating and refluxing extraction of 5 times of water gagings 2 times, each 1 hour, filter, tea grounds adds 5 times of water gagings, 100 ℃ of heating and refluxing extraction 0.5 hour, filter, tea grounds discards, and merge extractive liquid, filters, 70 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 75%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, 70 ℃ of reduced vacuum are dry, obtain Folium camelliae assamicae (processed) extract.
Embodiment 3
Get Folium camelliae assamicae (processed) 1kg, add 80 ℃ of heating and refluxing extraction of 12 times of water gagings 2 times, each 0.5 hour, filter, tea grounds adds 10 times of water gagings, 80 ℃ of heating and refluxing extraction 0.5 hour, filter, tea grounds discards, and merge extractive liquid, filters, 50 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 70%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, spraying is dry, obtains Folium camelliae assamicae (processed) extract.
Embodiment 4
Get Folium camelliae assamicae (processed) 10kg, add 50 ℃ of heating and refluxing extraction of 8 times of water gagings 2 times, each 3 hours, filter, tea grounds adds 8 times of water gagings, 50 ℃ of heating and refluxing extraction 1 hour, filter, tea grounds discards, and merge extractive liquid, filters, 60 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 60%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, spraying is dry, obtains Folium camelliae assamicae (processed) extract 1.1kg.
Embodiment 5
Get Folium camelliae assamicae (processed) 1kg, add 100 ℃ of heating and refluxing extraction of 20 times of water gagings 3 times, each 0.5 hour, filter, tea grounds adds 16 times of water gagings, 100 ℃ of heating and refluxing extraction 0.5 hour, filter, tea grounds discards, and merge extractive liquid, filters, 80 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, 70 ℃ of reduced vacuum are dry, obtain Folium camelliae assamicae (processed) extract 130g.
Embodiment 6
Get Folium camelliae assamicae (processed) 1kg, add 80 ℃ of heating and refluxing extraction of 16 times of water gagings 1 time, each 2 hours, filter, tea grounds adds 16 times of water gagings, 80 ℃ of heating and refluxing extraction 2 hours, filter, tea grounds discards, and merge extractive liquid, filters, 50 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 50%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, spraying is dry, obtains Folium camelliae assamicae (processed) extract 120g.
Embodiment 7
According to any one extract in embodiment 1-6, add conventional adjuvant, the conventional method on application galenic pharmacy, makes tablet.
Embodiment 8
According to any one extract in embodiment 1-6, add conventional adjuvant, the conventional method on application galenic pharmacy, makes capsule.
Embodiment 9
According to any one extract in embodiment 1-6, add conventional adjuvant, the conventional method on application galenic pharmacy, granulation agent.
Embodiment 10
According to any one extract in embodiment 1-6, add conventional adjuvant, the conventional method on application galenic pharmacy, makes powder.
Embodiment 11
According to any one extract in embodiment 1-6, add conventional adjuvant, the conventional method on application galenic pharmacy, makes drop pill.
Embodiment 12
According to any one extract in embodiment 1-6, add conventional adjuvant, the conventional method on application galenic pharmacy, makes suppository.
Embodiment 13
According to any one extract in embodiment 1-6, with common process, join by a certain percentage in tea product, make the health tea with decompression.
Embodiment 14
According to any one extract in embodiment 1-6, with common process, join by a certain percentage in milk product, make the health food with buck functionality.
Embodiment 15
Any one extract according in embodiment 1-6, joins in beverage by a certain percentage with common process, makes the health beverage with buck functionality.
Embodiment 16
Any one extract according in embodiment 1-6, joins in cake by a certain percentage with common process, makes the health care cake with buck functionality.
Claims (10)
1. a Folium camelliae assamicae (processed) extract, is characterized in that, by Folium camelliae assamicae (processed), is prepared as follows and is obtained: get Folium camelliae assamicae (processed), decoct with water, tea grounds discards, and adds ethanol standing after extracting solution is concentrated, precipitation separation, and supernatant is dry, obtains.
2. Folium camelliae assamicae (processed) extract as claimed in claim 1, it is characterized in that being prepared as follows and obtain: get Folium camelliae assamicae (processed) appropriate, add 5-20 times of water gaging heating and refluxing extraction 1-3 time, each 0.5-3 hour, filter, tea grounds adds 4-16 times of water gaging, heating and refluxing extraction 0.5-3 hour, filter, tea grounds discards, merge extractive liquid, filter, a filtrate 50-80 ℃ reduced vacuum is concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, adding 95% ethanol alcohol is sink to containing alcohol amount 50-80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, be drying to obtain.
3. Folium camelliae assamicae (processed) extract as claimed in claim 1, it is characterized in that being prepared as follows and obtain: get Folium camelliae assamicae (processed) appropriate, add 6-12 times of water gaging 50-100 ℃ heating and refluxing extraction 1-3 time, each 0.5-3 hour, filter, tea grounds adds 5-10 times of water gaging, 50-100 ℃ of heating and refluxing extraction 0.5-3 hour, filter, tea grounds discards, merge extractive liquid, filter, a filtrate 50-80 ℃ reduced vacuum is concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, adding 95% ethanol alcohol is sink to containing alcohol amount 50-80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, spraying is dry or reduced vacuum is dry, obtain.
4. Folium camelliae assamicae (processed) extract as claimed in claim 1, it is characterized in that being prepared as follows and obtain: get Folium camelliae assamicae (processed) appropriate, add 80 ℃ of heating and refluxing extraction of 10 times of water gagings 2 times, each 1 hour, filter, tea grounds adds 8 times of water gagings, 80 ℃ of heating and refluxing extraction 0.5 hour, filter, tea grounds discards, merge extractive liquid, cross 300 order filter clothes, 70 ℃ of reduced vacuum of filtrate are concentrated into relative density 1.05-1.10, tubular type is centrifugal, obtain centrifuged supernatant, adding 95% ethanol alcohol is sink to containing alcohol amount 80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.05-1.10, tubular type is centrifugal, obtain supernatant, spraying is dry or 70 ℃ of reduced vacuum are dry, obtain.
5. the Folium camelliae assamicae (processed) extract as described in as arbitrary in claim 1 ~ 4, its application in preparing Altace Ramipril or health food.
6. according to the application of claim 5, it is characterized in that, described application comprises takes Folium camelliae assamicae (processed) extract as the pharmaceutical composition of active constituents of medicine.
7. according to the application of claim 6, it is characterized in that described pharmaceutical composition is any pharmaceutically useful dosage form.
8. according to the application of claim 7, it is characterized in that, described medicine composition dosage form is selected from: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop or patch etc.
9. according to the application of claim 5, it is characterized in that, described health food is selected from: beverage, milk product, Folium Camelliae sinensis or cake etc.
10. the preparation method of Folium camelliae assamicae (processed) extract as claimed in claim 1, is characterized in that, step is as follows:
Get Folium camelliae assamicae (processed) appropriate, add 5-20 times of water gaging heating and refluxing extraction 1-3 time, each 0.5-3 hour, filters, tea grounds adds 4-16 times of water gaging, and heating and refluxing extraction 0.5-3 hour filters, and tea grounds discards, merge extractive liquid,, filters, and a filtrate 50-80 ℃ reduced vacuum is concentrated into relative density 1.02-1.15, centrifugal, obtain centrifuged supernatant, add 95% ethanol alcohol and be sink to containing alcohol amount 50-80%, hold over night, centrifugal, obtain precipitate with ethanol precipitation, heating water redissolves to proportion 1.02-1.15, centrifugal, obtain supernatant, be drying to obtain.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108323766A (en) * | 2017-01-19 | 2018-07-27 | 云南天士力帝泊洱生物茶集团有限公司 | A kind of tea dietary fiber and preparation method thereof with bowel relaxing functions |
| CN111629742A (en) * | 2017-11-20 | 2020-09-04 | 株式会社爱茉莉太平洋 | Composition comprising green tea extract with increased polysaccharide content as active ingredient |
| CN113812482A (en) * | 2021-09-27 | 2021-12-21 | 四川茗通天下茶业有限公司 | Preparation method of dark tea product |
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| CN101961060A (en) * | 2009-07-24 | 2011-02-02 | 天津天士力现代中药资源有限公司 | Pu'er tea extract and preparation method and application |
| CN101961059A (en) * | 2009-07-24 | 2011-02-02 | 天津天士力现代中药资源有限公司 | Puer tea extract, preparation method and application |
| CN102302068A (en) * | 2011-09-21 | 2012-01-04 | 湖南中医药大学 | Tea beverage for preventing and treating metabolic syndrome |
| CN103222520A (en) * | 2012-01-31 | 2013-07-31 | 云南天士力帝泊洱生物茶集团有限公司 | Low-caffeine tea powder and preparation method |
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| CN101961060A (en) * | 2009-07-24 | 2011-02-02 | 天津天士力现代中药资源有限公司 | Pu'er tea extract and preparation method and application |
| CN101961059A (en) * | 2009-07-24 | 2011-02-02 | 天津天士力现代中药资源有限公司 | Puer tea extract, preparation method and application |
| CN102302068A (en) * | 2011-09-21 | 2012-01-04 | 湖南中医药大学 | Tea beverage for preventing and treating metabolic syndrome |
| CN103222520A (en) * | 2012-01-31 | 2013-07-31 | 云南天士力帝泊洱生物茶集团有限公司 | Low-caffeine tea powder and preparation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108323766A (en) * | 2017-01-19 | 2018-07-27 | 云南天士力帝泊洱生物茶集团有限公司 | A kind of tea dietary fiber and preparation method thereof with bowel relaxing functions |
| CN108323766B (en) * | 2017-01-19 | 2023-06-02 | 云南天士力帝泊洱生物茶集团有限公司 | Tea dietary fiber with function of relaxing bowels and preparation method thereof |
| CN111629742A (en) * | 2017-11-20 | 2020-09-04 | 株式会社爱茉莉太平洋 | Composition comprising green tea extract with increased polysaccharide content as active ingredient |
| CN113812482A (en) * | 2021-09-27 | 2021-12-21 | 四川茗通天下茶业有限公司 | Preparation method of dark tea product |
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| CN103505564B (en) | 2018-06-12 |
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