CN102727663A - Application of Pu'er tea extract in preparing hypotensive medicine or foodstuff - Google Patents
Application of Pu'er tea extract in preparing hypotensive medicine or foodstuff Download PDFInfo
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Abstract
The invention relates to an application of a Pu'er tea extract in preparing hypotensive medicines or foodstuffs. The invention belongs to the fields of pharmacy and foodstuff. An extraction method provided by the invention comprises the two steps: step 1, Pu'er tea leaves are decocted by using water; an extraction liquid is filtered and condensed until a certain proportion is reached; and step 2, the concentrated solution is subjected to centrifugation, a centrifugate is concentrated and dried, such that the extract is obtained.
Description
Technical field
The present invention relates to a kind of Pu'er tea in preparation Altace Ramipril or Application in Food, belong to pharmacy and field of health care food.
Background technology
The Folium camelliae assamicae original producton location is mainly in the Simao Diqu and Xishuangbanna in Yunnan.Along with the popularization of large leaf, Sichuan, Guangdong, Guangxi also become the province that Folium camelliae assamicae is produced.Along with expanding economy, the enhancing of people's health care consciousness, Folium camelliae assamicae is sweet because of mellow time of its flavour, and the unique fine quality of CHENXIANG plays the concern and the attention of the whole society with its special health-care efficacy to human body, and product receives the favor of consumer deeply.
Warm in nature and the storage tolerance of Folium camelliae assamicae is suitable for cooking and uses or the bubble drink.The many historical book of Ancient Times in China have a lot of records about the Folium camelliae assamicae effect: the record of ZHAO Xue-Min supplementary Amplifications of the Compendium of Materia Medica, and Pu Hei sobers up the first like lacquer, eliminates indigestion and phlegm, and clearing stomach is promoted the production of body fluid, and power is especially greatly also; Cloud again in " woody part ", Pu'er tea paste can be controlled all kinds of diseases and ailments, catches cold like tripe, disperses with ginger decoction, and perspiration can be healed, and mouthful broken larynx is dry, and the pain of being heated was chewed with five minutes and promptly to be healed the night of making a slip of the tongue; Chen Zonghai writes, and " Pu'er facilitating digestion disperses cold, and Detoxication is arranged in " interview of the Room, Simao " record." clear scholar Song male " occupying the diet spectrum with breath " cloud " Pu'er product person, the highly seasoned kind wind-phlegm meat that disappears of telling, all filthy pathogen in summer string-shaped mass in the abdomen stomachache, diseases such as cholera dysentery are often healed from the beginning of, drink ".This shows that forefathers think to the Pu'er tea health-care experience, Folium camelliae assamicae has to help digestion and removes poison, and regulate the flow of vital energy and expand, removing heat-phlegm, wind dispelling is sobered up, and controls effects such as dysentery is antibacterial.
Present research thinks that Folium camelliae assamicae has the health-care efficacy of several respects:
1, lowering blood-fat and reducing weight: Folium camelliae assamicae is in close relations with the metabolism of fat; Folium camelliae assamicae has generated new chemical substance through unique sweat; The lipase that contains lipase that wherein has can produce decomposition to fat, thereby Folium camelliae assamicae has the effect of fat-reducing.
2, blood pressure lowering, arteriosclerosis
3, anti-cancer, anticancer: scientist through a large amount of crowds relatively proves that the crowd's of drinking tea cancer morbidity is lower.And Folium camelliae assamicae contains multiple abundant anticancer trace element, and the tumoricidal effect of Folium camelliae assamicae is strong.
4, strong protecting teeth: contain many physiologically active ingredients in the Folium camelliae assamicae, have the effect of sterilizing, therefore can remove halitosis, take care of one's teeth.
5, protect stomach, nourishing the stomach: under suitable concentration; Drink gentle Folium camelliae assamicae the intestines and stomach is not produced stimulation; The film that thickness, sweet cunning, mellow Folium camelliae assamicae entering human body the intestines and stomach form adheres to the top layer of stomach; Stomach is produced useful protective layer, secularly can play the effect of protecting stomach, nourishing the stomach after drinking Folium camelliae assamicae.This is to advocate consumer's appellation Folium camelliae assamicae of drinking Folium camelliae assamicae main cause for " beauty treatment tea ", " tea lengthens one's life " both at home and abroad.
6, defying age: prove that after deliberation the lipid peroxidation process is one of mechanism of human senility in the human body.The vitamin C that contains in the Folium camelliae assamicae, vitamin E, tea polyphenols, aminoacid and trace element etc. have antioxidation, delaying senility course, so Folium camelliae assamicae is called as " tea lengthens one's life ".
7, radioprotective: the result of study of carrying out with Folium camelliae assamicae according to what state's fence of Guangdong Zhongshan University etc. shows, drinks 2% Folium camelliae assamicae and can remove the injury that causes with co-60 radiation.
8, sober up: supplementary Amplifications of the Compendium of Materia Medica carries: " general tea is controlled oil most and is covered pericardium, postcibal diarrhea, sobers up first." medical evidence: the tea polyphenols in the Folium Camelliae sinensis can promote alcohol metabolism, and liver is had protective effect.Alcohol metabolism can normally be carried out smoothly.Having tea to increase the vasoconstriction function.Theophylline has diuresis, can impel ethanol to excrete fast, reduces the harm after drunk.Drink tea and can also replenish the required vitamin C of ethanol hydrolysis, excited by the big mesencephalic centre of ethanol anesthesia.Thereby play antialcoholism action.
The lipid-reducing function of Folium camelliae assamicae receives wide acceptance at present.But hypotensive effect never has direct experimental data support.2007 even relevant report is arranged negates the hypotensive effect of Folium camelliae assamicae.
Because at present consumption habit just brews with Folium Camelliae sinensis and drinks, functional component is brewed number of times, brews temperature, brewing time etc. influences and causes stripping difference, can not utilize fully.
Summary of the invention
The present invention provides a kind of Pu'er tea and preparation method thereof.
The present invention also provide pharmaceutical composition of containing extract of the present invention and preparation method thereof with and the medicine of preparation blood pressure lowering or the purposes in the health food.
Pu'er tea of the present invention obtains through extracting processing, its method for distilling, and step is following:
Step 1, the puerh tea leaves decocte with water, extracting liquid filtering is concentrated to certain weight proportion;
Step 2, concentrated solution is centrifugal, and centrifugal liquid concentrates, and is drying to obtain.
Preferably, method for distilling of the present invention, step is following:
Step 1, the puerh tea leaves decocte with water is extracted 2-4 time, and each 0.5～2 hour, the water of 6-12 times of volume; Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2-1: 3,
Step 2, concentrated solution is centrifugal with centrifuge, and centrifugal liquid is evaporated to proportion 1.1-1.25 (45-65 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
It is preferred,
Method for distilling of the present invention, step is following:
Step 1, puerh tea leaves add water acutely seethes with excitement and decoct to extract 3 times, and each 0.5～2 hour, the water of 6-12 times of volume; Preferably (1.5h, 1.5h, 1h; 10 times of volumes, 8 times of volumes, 8 times of volumes).Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2-1: 3,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.1-1.25 (45-65 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
Tubular type centrifugal condition wherein: centrifuge speed: 15000-19000 commentaries on classics/min; Spray drying condition: EAT: 140-190 ℃, leaving air temp: 75-95 ℃
The extract of the present invention that the above method of process obtains can be used as the active component with buck functionality and is prepared into pharmaceutical composition or health food.Health food of the present invention includes but not limited to: beverage, milk product, Folium Camelliae sinensis, cake etc.
Active constituents of medicine in the pharmaceutical composition of the present invention, its shared percentage by weight in compositions can be 0.1-99.9%, all the other are the medicine acceptable carrier.Pharmaceutical composition of the present invention exists with unit dosage form, and said unit dosage form is meant the unit of preparation, as every of tablet, and capsular every capsules, every bottle of oral liquid, every bag of granule etc.
Pharmaceutical composition of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, peroral dosage form preferably, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc.
Pharmaceutical composition of the present invention, the preparation of its oral administration can contain excipient commonly used, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet in case of necessity.
The filler that is suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of appropriate drug comprises sodium lauryl sulphate.
Can fill through mixing, the method that tabletting etc. are commonly used prepares solid oral composition.Mix repeatedly active substance is distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid for example can be aqueous or oily suspensions, solution, Emulsion, syrup or elixir, perhaps can be a kind of available water before use or other suitable composite dry products of carrier.This liquid preparation can contain conventional additive; Such as suspending agent; For example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat; Emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if desired, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, can this chemical compound be suspended or dissolving.The preparation of solution is normally through being dissolved in active substance in a kind of carrier filter-sterilized before it is packed into a kind of suitable bottle or ampoule, sealing then.For example a kind of local anesthetic of adjuvant, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after the bottle of packing into that this compositions is freezing, and under vacuum, water is removed.
Pharmaceutical composition of the present invention; When being prepared into medicament, optionally add suitable medicine acceptable carrier; Said medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDTA disodium, EDTA calcium sodium, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Compositions of the present invention is confirmed usage and dosage according to patient's situation in use, but obeys every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet.
The present invention also comprises the application of Pu'er tea of the present invention in the preparation Altace Ramipril.
Pu'er tea of the present invention has good health care and treatment function, particularly aspect blood pressure lowering.
Below through the experiment of spontaneous hypertension (SHR) rat experiment and high fructose Hypertensive Rats, totally two animal hypertension models, two batches of experimental datas are explained beneficial effect of the present invention:
(1) Pu'er tea is to the blood pressure lowering dose-effect research experiment of spontaneous hypertension rat (SHR)
1 experiment material
1. " tried thing: Pu'er tea is made by embodiment 1 said method.Positive drug adopts irbesartan, and (the irbesartan sheet, France produces, the packing of Hangzhou Sanofi-Aventis people's livelihood pharmaceutical Co. Ltd.Lot number of the repackaged products: 0906193. batch number: 1797. dates of manufacture: 2009.02).
2. laboratory animal: adopt spontaneous hypertension rat (SHR), available from Beijing Vital River Experimental Animals Technology Co., Ltd., the quality certification number: SCXK (capital) 2006-0009.The raising condition: raise in air-conditioned animal housing, temperature 20-25 ℃, relative humidity 40%-60%, 5 in every cage adds feedstuff at regular time and quantity, and edible rat special feed is freely drunk water, and changes bedding and padding every day.
3. instrument: the non-invasive blood pressure appearance (CODA of U.S. Kent company
TM2).
2 experimental techniques
1. experiment is divided into groups: totally six groups: model group, positive drug group, Pu'er tea 1,2,3,4 dose groups.Each group is the SHR rat, 10 every group.
2. dosage design: each kind dosage all calculates according to the crude drug amount, obtains following dosage every day.
Table 1 Pu'er tea is respectively organized dosage design table to SHR blood pressure lowering experiment
| Group | Administration every day situation | The administration volume |
| Positive group | Irbesartan (15.5mg/kg) | 1ml/100g |
| Model group | Tap water | 1ml/100g |
| Pu'er tea 1 dose groups | Irritate stomach, Pu'er tea 0.25g/kg. | 1ml/100g |
| Pu'er tea 2 dose groups | Irritate stomach, Pu'er tea 0.5g/kg. | 1ml/100g |
| Pu'er tea 3 dose groups | Irritate stomach, Pu'er tea 1g/kg. | 1ml/100g |
| Pu'er tea 4 dose groups | Irritate stomach, Pu'er tea 2g/kg. | 1ml/100g |
3. experimental program: animal is raised a week after arriving laboratory in advance, measures basic blood pressure (basic blood pressure need be measured 1-2 week, guarantees that animal has adapted to instrument, and the blood pressure that measures is a true value).According to the basic blood pressure random packet.After the grouping, begin experiment next day, positive drug group, each group of Pu'er tea and model group are pressed the 1ml/100g gastric infusion, successive administration one month.Measure blood pressure weekly once during this time.And grasp the time, guarantee that animal measuring basically at the same time, avoid in the daytime the influence of self fluctuation of blood pressure.Administration stops administration after one month, in convalescent period, the fluctuation situation of blood pressure detects twice weekly after the observation drug withdrawal, continues 1-2 week (looking concrete blood pressure situation).
4. observation index: MAIN OUTCOME MEASURES: blood pressure comprises systolic pressure, diastolic pressure, mean arterial pressure.Other indexs: body weight, heart rate, blood biochemical.
3 experimental results
1. give a month Pu'er tea continuously, the variation of animal mean arterial pressure
According to experimental result, in successive administration one month, after one week of administration, the mean arterial pressure of positive drug group and tea extract 2.0g/kg group significantly reduces and model group more all has significance reduction (p＜0.05), around the significant difference of its reduction continues.And the mean arterial pressure of tea extract 1.0g/kg group has the trend of reduction, is all in around one week of administration and the administration twice testing result and model group and relatively has significance reduction (p＜0.05).The detected value and the model group of tea extract 0.25 and 0.5g/kg group compare, all unknown significance difference.See table 2.
Table 2 an administration influence to each treated animal mean arterial pressure in month.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
2. the variation of long term administration animal systolic pressure
According to experimental result; In successive administration one month; The systolic pressure of positive drug group, tea extract 1.0g/kg group and tea extract 2.0g/kg group all has reduction; More all have significance with model group and reduce (p＜0.05), around wherein the significant difference of positive drug and tea extract 2.0g/kg group reduction continues.The detected value and the model group of tea extract 0.25 and 0.5g/kg group compare, all unknown significance difference.See table 3.
Table 3 long term administration is to the influence of each treated animal systolic pressure.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
3. the diastolic pressure of long term administration experiment changes
According to experimental result; In successive administration one month; The diastolic pressure of positive drug group, tea extract 1.0g/kg group and tea extract 2.0g/kg group all has reduction; More all have significance with model group and reduce (p＜0.05), around wherein the significant difference of positive drug and tea extract 2.0g/kg group reduction continues.The detected value and the model group of tea extract 0.25 and 0.5g/kg group compare, all unknown significance difference.See table 4.
Table 4 long term administration is to the influence of each treated animal diastolic pressure.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
4. the changes in heart rate of long term administration experiment
Administration one month, all within normal range, the result sees table 5 to the heart rate of each treated animal.
The heart rate influence of table 5 long term administration blood pressure lowering experiment.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
5. body weight change during the administration.
Administration one month, the body weight and the model group of last two all tea extract 2.0g/kg groups relatively increases slowly, and exist but increase still, and in the normal type scope of animal, therefore think that this species diversity does not have biological significance.
Table 6 long term administration is to the influence of each treated animal body weight.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
6. convalescent period animal blood pressure, heart rate and body weight testing result
In three weeks of drug withdrawal, the mean arterial pressure of Pu'er tea 2.0g/kg group and positive drug group still significantly is lower than model group (p＜0.05), and and the pressure reduction of model group about 20mmHg.Heart rate and body weight all in normal range, do not have significant change.See table 7.
Each item of animal detects the index situation after one week of table 7 drug withdrawal.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
Each item of animal detects the index situation after two weeks of table 8 drug withdrawal.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
Each item of animal detects the index situation after three weeks of table 9 drug withdrawal.mean±SD
Annotate: and with model group constantly relatively:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
7. after three convalescent periods in week, the blood biochemical of animal changes.
Experience four administration weeks and three recovery weeks, the blood glucose value of tea extract administration treated animal slightly rises, but does not have biological significance.Two of Protein Detection indexs all do not have significant change.Two of lipids detection indexs all do not have significant change.Detected value is all in the normal physiological scope.Explain give Pu'er tea after, through after a while recovery, it is normal that animal each item blood parameters recovers.
After convalescent periods in table 10 three week, the blood biochemical of animal changes (mean ± SD)
Annotate: compare with model group:
*: p＜0.05;
*: p＜0.01;
* *: p＜0.001
4 conclusions
The continuous irrigation stomach gives one month; After one week of administration; The mean arterial pressure of positive drug group, tea extract 1.0g/kg group and tea extract 2.0g/kg group, systolic pressure, diastolic pressure all significantly reduce and model group more all has significance and reduces (p＜0.05), and pressure reduction reaches 20mmHg.The detected value and the model group of tea extract 0.25 and 0.5g/kg group compare, all unknown significance difference.Can judge for SHR rat model thus, irritate stomach and give Pu'er tea that the onset dosage of antihypertensive effect is 1.0g/kg.
The continuous irrigation stomach gives Pu'er tea 2.0g/kg, and the body weight and the model group of this treated animal relatively increases slowly, and exists but increase still, and in the normal type scope of animal, therefore thinks that this species diversity does not have biological significance.And during the administration, the heart rate of this treated animal maintains higher level, perhaps be that metabolic rate is higher because heart rate is higher, thereby the body weight gain that causes animal is slow.But perhaps this is the performance of certain toxic and side effects of Pu'er tea, needs further experiment to verify.
In three weeks of drug withdrawal, the mean arterial pressure of Pu'er tea 2.0g/kg group and positive drug group still significantly is lower than model group (p＜0.05), and and the pressure reduction of model group about 20mmHg.Heart rate and body weight all in normal range, do not have significant change.The blood pressure lowering that Pu'er tea is described is a permanent mechanism.Perhaps, at present the blood pressure lowering mechanism of Pu'er tea not being studied, is the regulating action to integral body, perhaps is to have influenced Expression of Related Genes.Remaining follow-up experimentation explores.
Experience four administration weeks and three recovery weeks, the blood glucose value of tea extract administration treated animal slightly rises, but does not have biological significance.Two of Protein Detection indexs all do not have significant change.Two of lipids detection indexs all do not have significant change.Detected value is all in the normal physiological scope.Explain give Pu'er tea after, through after a while recovery, animal each item blood parameters is normal.
(2) Pu'er tea is to the blood pressure lowering dose-effect research experiment of high fructose Hypertensive Rats
1 material and method
1. animal: 60 of SD rats, male, body weight (180 ± 20) g; Available from Beijing Vital River Experimental Animals Technology Co., Ltd., the quality certification number: SCXK (capital) 2006-0009.Raising condition: temperature 20-25 ℃, relative humidity 40%-60%.
2. high fructose drinking-water: use fresh tap water every day, dispose 10% high fructose drinking-water.Water appliance cleans every day, and high-temperature sterilization is once avoided breed bacteria weekly.
3. tried thing and key instrument: Pu'er tea is made by embodiment 1 said method.The positive drug irbesartan, France produces (packing of Hangzhou Sanofi-Aventis people's livelihood pharmaceutical Co. Ltd), lot number of the repackaged products: 0906193 (batch number: 1797).Fructose: U.S. Tate&Lyle Ingredients company, lot number: TK1B091C.Used key instrument: the non-invasive blood pressure appearance (CODA of U.S. Kent company
TM2).
4. dosage setting and grouping
According to preliminary experiment result in early stage, Pu'er tea is low, high dose group is respectively 0.5g/kg and 1.0g/kg, and irbesartan dosage is 15.5mg/kg.Fresh configuration every day, the filling stomach amount of animal is 1ml/100g.
60 rats are divided into 5 groups at random, and 12 every group: the normal control group (N) give tap water; Model group (M), positive drug group (C), low dose group (L promptly), high dose group (H) in addition, animal give 10% fructose drinking-water every day and freely drink and make hypertension model, survey body weight and blood pressure weekly once.Compare with the N group, hypertension 20mmHg, and have statistical significant difference (p＜0.05) for the modelling success, at this moment the part animal is rejected 10 every group.
The N group continues to give light water, and all the other 4 groups are continued to give high fructose drinking-water; Different group animal gives correspondingly to be tried thing 1 time every day, totally 4 weeks.
5. observation index: duration of test is weighed once weekly.Begin to measure blood pressure weekly once around the fructose the from giving.And grasp the time, guarantee that animal measuring basically at the same time, avoid in the daytime the influence of self fluctuation of blood pressure.Measure blood parameters after the off-test.
6. statistical method: adopt the SPSS11.0 statistical software that test data is carried out statistical procedures.
2 experimental results
2.1 body weight change
During giving high fructose, the equal sustained, stable growth of each treated animal body weight.The body weight of modeling the 6th each high fructose group during week all has with compared with normal and exceeds, and wherein L organizes and normal group has had significant difference (p＜0.05) more.Can't carry out non-invasive blood pressure after the weight of animals is too big and detect, the interpolation of unified control feedstuff after therefore weighing since the 6th week, but do not control drinking-water, control the weight of animals whereby.The body weight of finding animal of weighing in the 8th week of modeling is controlled to some extent, but the body weight of each group of high fructose still is higher than normal group, and C, two groups of L and normal group relatively have significant difference (p＜0.05) (table 11).
After beginning to be tried thing, obvious influence is not seen in the growth of the weight of animals, still be significantly higher than normal group respectively for the body weight of high fructose group, significant difference is arranged.Therefore and the 3rd week alleviated (table 12) more to some extent owing to need the fasting blood sampling, body weight is that fasting was claimed after 12 hours around.
Table 11 gives the SD rat body weight change list mean ± SD of high fructose modeling, g
Annotate: n=12; Compare with normal group:
*P＜0.05,
*P＜0.01.
Table 12 is tried respectively organizes rat body weight change list mean ± SD, g behind the thing
Annotate: n=10; Compare with normal group:
*P＜0.05,
*P＜0.01.
2.2 blood pressure
Use the non-invasive blood pressure appearance to measure animal blood pressure, because mean arterial pressure is clinical bioassay standard, the therefore unified mean arterial pressure data that adopt.
During giving high fructose; The equal sustainable growth of each high fructose treated animal mean arterial pressure, through eight all modelings, the blood pressure of high fructose group improves 20mmHg than matched group; Relatively have significant difference (p＜0.05) with normal group, cause rat hypertension model (table 13).
After beginning to be tried thing, the mean arterial pressure of positive drug treated animal relatively has significant difference (p＜0.05) one week of administration just having occurred obvious decline and model group in, and be in this scope of data in around administration always and normal group similar.Administration three week the back and the around Pu'er tea 1.0g/kg can significantly reduce the animal mean arterial pressure and model group relatively has significant difference (p＜0.05) (table 14).Pu'er tea 0.5g/kg does not see obvious hypotensive effect.
Table 13 gives the SD rat blood pressure change list mean ± SD of high fructose modeling, mmHg
Annotate: n=12; Compare with normal group:
*P＜0.05,
*P＜0.01.
Table 14 is tried respectively organizes rat blood pressure change list mean ± SD, mmHg behind the thing
Annotate: n=10; Compare with normal group:
*P＜0.05,
*P＜0.01; Compare with model group: #P＜0.05, ##P＜0.01;
3 discussion of results
Through eight all modelings, the blood pressure of high fructose group improves 20mmHg than matched group and normal group relatively has significant difference, confirms as and causes rat hypertension model.Administration three week the back and the around positive drug, Pu'er tea 1.0g/kg can significantly reduce the animal mean arterial pressure and model group relatively has significant difference, thinks that Pu'er tea 1.0g/kg has the blood pressure lowering effect to this animal pattern.
Advantage of the present invention is: a kind of Pu'er tea is provided, and this extract evidence has good antihypertensive function, and active constituent content is high simultaneously; Steady quality, easy to carry, be convenient to take; Can be used for preparing various health foods or health tea, play good antihypertensive effect.
Description of drawings
Fig. 1 is an administration influence to each treated animal mean arterial pressure of SHR in month
Fig. 2 is that long term administration is to each treated animal changes in heart rate of SHR
Fig. 3 is the body weight change of month each treated animal of SHR of administration
The specific embodiment
Below further specify the present invention through embodiment, but not as limitation of the present invention, the water extract of Folium camelliae assamicae all has effect of the present invention.
Embodiment 1
The Pu'er tea production technology:
Puerh tea leaves adds water and acutely seethes with excitement and decoct to extract 3 (1.5h, 1.5h, 1h; 10bv, 8bv, 8bv); Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2-1: 3, concentrated solution is centrifugal with tripod pendulum type batch centrifugal; Tripodia centrifugal liquid is centrifugal with tube centrifuge; Centrifugal liquid is evaporated to proportion 1.1-1.25 (45-65 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
Tubular type centrifugal condition wherein: centrifuge speed: 17000 commentaries on classics/min; Spray drying condition: EAT: 160 ℃, leaving air temp: 85 ℃.
Embodiment 2
Step 1, the puerh tea leaves decocte with water is extracted 3 times, and each 0.5 hour, the water of 6 times of volumes; Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2,
Step 2, concentrated solution is centrifugal with centrifuge, and centrifugal liquid is evaporated to proportion 1.1 (45 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
Embodiment 3
Step 1, the puerh tea leaves decocte with water is extracted 3 times, and each 2 hours, the water of 12 times of volumes; Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 3,
Step 2, concentrated solution is centrifugal with centrifuge, and centrifugal liquid is evaporated to proportion 1.25 (65 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
Embodiment 4
Step 1, puerh tea leaves add water acutely seethes with excitement and decoct to extract 3 times, and each 0.5 hour, the water of 6 times of volumes; Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.1 (45 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
Tubular type centrifugal condition wherein: centrifuge speed: 15000 commentaries on classics/min; Spray drying condition: EAT: 140 ℃, leaving air temp: 75 ℃
Embodiment 5
Step 1, puerh tea leaves add water acutely seethes with excitement and decoct to extract 3 times, and each 2 hours, the water of 12 times of volumes.Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 3,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.25 (65 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).
Tubular type centrifugal condition wherein: centrifuge speed: 19000 commentaries on classics/min; Spray drying condition: EAT: 190 ℃, leaving air temp: 95 ℃
Embodiment 6
Puerh tea leaves adds water and acutely seethes with excitement and decoct to extract 3 (1h, 1h, 1h; 6bv, 6bv, 6bv); Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2.5, concentrated solution is centrifugal with tripod pendulum type batch centrifugal; Tripodia centrifugal liquid is centrifugal with tube centrifuge; Centrifugal liquid is evaporated to proportion 1.1-1.25 (50 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).Tubular type centrifugal condition wherein: centrifuge speed: 17000 commentaries on classics/min; Spray drying condition: EAT: 160 ℃, leaving air temp: 85 ℃
Embodiment 7
Puerh tea leaves adds water and acutely seethes with excitement and decoct to extract 3 (2h, 2h, 2h; 10bv, 10bv, 10bv); Extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2.5, concentrated solution is centrifugal with tripod pendulum type batch centrifugal; Tripodia centrifugal liquid is centrifugal with tube centrifuge; Centrifugal liquid is evaporated to proportion 1.1-1.25 (50 ℃), and the condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).Tubular type centrifugal condition wherein: centrifuge speed: 17000 commentaries on classics/min; Spray drying condition: EAT: 160 ℃, leaving air temp: 85 ℃
Embodiment 8
Puerh tea leaves adds water and acutely seethes with excitement and decoct to extract 2 (0.5h, 0.5h; 12bv, 12bv), extracting liquid filtering; Filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2.5; Concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.1-1.25 (50 ℃); The condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).Tubular type centrifugal condition wherein: centrifuge speed: 17000 commentaries on classics/min; Spray drying condition: EAT: 160 ℃, leaving air temp: 85 ℃
Embodiment 9
Puerh tea leaves adds water and acutely seethes with excitement and decoct to extract 4 (2h, 2h, 2h, 2h; 10bv, 10bv, 10bv; 10bv), extracting liquid filtering, filtrate decompression concentrates (≤70 ℃) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1: 2.5; Concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.1-1.25 (50 ℃); The condensed cream spray drying promptly gets (or microwave drying, pulverize promptly get).Tubular type centrifugal condition wherein: centrifuge speed: 17000 commentaries on classics/min; Spray drying condition: EAT: 160 ℃, leaving air temp: 85 ℃
Embodiment 10
According to any extract among the embodiment 1-9, add conventional adjuvant, the conventional method on the application preparation is processed tablet.
Embodiment 11
According to any extract among the embodiment 1-9, add conventional adjuvant, the conventional method on the application preparation is processed capsule.
Embodiment 12
According to any extract among the embodiment 1-9, add conventional adjuvant, the conventional method on the application preparation is processed granule.
Embodiment 13
According to any extract among the embodiment 1-9, add conventional adjuvant, the conventional method on the application preparation is processed powder.
Embodiment 14
According to any extract among the embodiment 1-9, add conventional adjuvant, the conventional method on the application preparation is processed drop pill.
Embodiment 15
According to any extract among the embodiment 1-9, add conventional adjuvant, the conventional method on the application preparation is processed suppository.
Embodiment 16
According to any extract among the embodiment 1-9, join by a certain percentage in the tea product with common process, process health tea with decompression.
Embodiment 17
According to any extract among the embodiment 1-9, join by a certain percentage in the milk product with common process, process health food with buck functionality.
Embodiment 18
Any extract according among the embodiment 1-9 joins in the beverage with common process by a certain percentage, processes the health beverage with buck functionality.
Embodiment 19
Any extract according among the embodiment 1-9 joins in the cake with common process by a certain percentage, processes the health care cake with buck functionality.
Claims (10)
1. the application of Pu'er tea in preparation treatment Altace Ramipril or health food.
2. according to the application of claim 1, it is characterized in that the application in preparation treatment spontaneous hypertension or hypertensive medicine of high fructose or health food.
3. according to the application of claim 1, it is characterized in that said Pu'er tea is the Folium camelliae assamicae water solubility extract.
4. according to the application of claim 1, it is characterized in that, said Pu'er tea, extraction step is following:
Step 1, the puerh tea leaves decocte with water is extracted 2-4 time, and each 0.5～2 hour, the water of 6-12 times of volume; Extracting liquid filtering, filtrating is evaporated to Folium Camelliae sinensis weight under≤70 ℃ of conditions: concentrated solution volume=1: 2-1: 3,
Step 2, concentrated solution is centrifugal with centrifuge, and centrifugal liquid is evaporated to 45-65 ℃ of proportion 1.1-1.25, and condensed cream spray drying or microwave drying promptly get.
5. according to the application of claim 1, it is characterized in that, said Pu'er tea, extraction step is following:
Step 1, puerh tea leaves add water acutely seethes with excitement and decoct to extract 3 times, and each 0.5～2 hour, the water of amount of water 6-12 times volume; Extracting liquid filtering, filtrating is evaporated to Folium Camelliae sinensis weight under≤70 ℃ of conditions: concentrated solution volume=1: 2-1: 3,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to 45-65 ℃ of proportion 1.1-1.25, and condensed cream spray drying or microwave drying promptly get;
Tubular type centrifugal condition wherein: centrifuge speed: 15000-19000 commentaries on classics/min; Spray drying condition: EAT: 140-190 ℃, leaving air temp: 75-95 ℃.
6. according to the application of claim 1, it is characterized in that, said Pu'er tea, extraction step is following:
Puerh tea leaves adds water and acutely seethes with excitement and decoct to extract 3 times, decocts 1.5h, adds water 10bv for the 1st time; Decoct 1.5h, add water 8bv for the 2nd time; Decoct 1h, add water 8bv, extracting liquid filtering for the 3rd time; Filtrating is evaporated to Folium Camelliae sinensis weight under≤70 ℃ of conditions: concentrated solution volume=1: 2-1: 3; Concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to 45-65 ℃ of proportion 1.1-1.25; Condensed cream spray drying or microwave drying promptly get;
Tubular type centrifugal condition wherein: centrifuge speed: 15000-19000 commentaries on classics/min; Spray drying condition: EAT: 140-190 ℃, leaving air temp: 75-95 ℃.
7. according to the application of claim 1, it is characterized in that said application comprises takes the pharmaceutical composition of Pu'er tea as active constituents of medicine.
8. according to the application of claim 7, it is characterized in that said pharmaceutical composition is any pharmaceutically useful dosage form.
9. according to Claim 8 application; It is characterized in that said medicine composition dosage form is selected from: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop or patch.
10. according to the application of claim 1, it is characterized in that said health food is selected from: beverage, milk product, Folium Camelliae sinensis or cake.
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| CN2012100978142A CN102727663A (en) | 2011-04-14 | 2012-04-06 | Application of Pu'er tea extract in preparing hypotensive medicine or foodstuff |
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