CN103893593B - Pharmaceutical composition with effects of clearing away heat and toxic materials, resisting inflammations and boosting immunity - Google Patents

Pharmaceutical composition with effects of clearing away heat and toxic materials, resisting inflammations and boosting immunity Download PDF

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CN103893593B
CN103893593B CN201410134543.2A CN201410134543A CN103893593B CN 103893593 B CN103893593 B CN 103893593B CN 201410134543 A CN201410134543 A CN 201410134543A CN 103893593 B CN103893593 B CN 103893593B
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梁卫
刘华章
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Abstract

The invention belongs to the field of Chinese pharmaceutical manufacturing and relates to a pharmaceutical composition with effects of clearing away heat and toxic materials, resisting inflammations and boosting immunity and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 1-25 parts of golden camellia, 1-25 parts of honeysuckle flowers, 1-40 parts of jasmine, 1-50 parts of lotus leaves, 1-50 parts of folium mori, 1-50 parts of lophatherum gracile, 1-50 parts of lalang grass rhizome, 1-40 parts of chrysanthemum, 1-40 parts of mint, 1-50 parts of rabdosia lophanthide and 1-60 parts of strigose hydrangea juvenile leaves. The pharmaceutical composition has the effects of clearing away heat and toxic materials, resisting inflammations and boosting immunity and can be used for treating diseases such as cold and heatstroke and swollen sore throat caused by wind-heat common cold, internal heat and immunodeficiency and hot eyes, pain of eyes and facial acne caused by liver heat. The pharmaceutical composition is not only remarkable in curative effect, but also is extremely low in toxicity.

Description

Pharmaceutical composition with the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity
Technical field
The invention belongs to field of traditional Chinese medicine pharmacy, is related to a kind of medicine with the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity Compositions and preparation method thereof.
Background technology
With the quickening of socialization's rhythm, especially operating pressure constantly increases the working strength of modern, along with life Irregular, the situation such as such as Jing often stays up late, diet is irregular, smoking is drunk, monophagia is rich of state living, often causes dry Heat, liver-fire rise, and body immunity decline situation.Such as most people with have a stomach-ache, be hard and dry, having a sleepless night, female Property have a symptoms such as distending pain of the breast, and urbanite's more and more obvious emotion such as agitation, irritability is all caused to physical and mental health Strong influence.
At present people are for the daily health caring of city disease is mainly based on " reducing internal heat ", i.e. the function of heat-clearing and toxic substances removing.In for example A kind of camellia chrysantha chrysanthemum herb tea disclosed in state's patent CN1739371A, its prescription is made up of Camellia nitidissima Chi, Flos Chrysanthemi, Rhizoma Imperatae and Folium hydrangeae strigosae, and is made It is easy to take into teabag.The teabag has a function of damp eliminating, heat clearing away, toxin expelling, and the Chang Yinke spring is warm to dry, summer summer-heat quenches one's thirst, the autumn Day in solution dry, winter relieves inflammation or internal heat.It can be seen that, the teabag as a kind of health tea, its main function concentrate on damp eliminating, heat clearing away health care it Purposes.
But, cause the reason for general disease is non-single, also not single effect can be solved, and solve daily The key of health care also not simple heat clearing away and internal heat reducing, it is often more important that build immunity of human body itself, from root solve problem.
The content of the invention
It is an object of the invention to provide a kind of drug regimen for having the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity concurrently Thing, and further disclose its preparation method.
Present invention also offers aforementioned pharmaceutical compositions are being prepared with the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity Application in medicine.
The purpose of the present invention is achieved through the following technical solutions:
A kind of crude drug group of pharmaceutical composition with the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity of the present invention Become:
The above-mentioned pharmaceutical composition with the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity is preferably by following crude drug group Into:
The above-mentioned pharmaceutical composition with the effect of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity is more preferably by following crude drug group Into:
Pharmaceutical composition of the present invention adds customary adjuvant, according to common process, make clinically acceptable bag tea agent, Distillate medicinal water, oral liquid formulations, tablet, granule, capsule, mixture, pill or drop pill.
Present invention also offers the preparation method of described pharmaceutical composition, takes the JINHUA including according to selected weight portion Tea, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, crush after mixing or crush After mix, and selectivity addition customary adjuvant, according to common process, obtain final product bag tea agent.
The preparation method of pharmaceutical composition of the present invention, takes the Camellia nitidissima Chi, Flos Lonicerae, Cape jasmine including according to selected weight portion Son, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, and plus Enter the distilled water immersion 2-4 hours that medical material gross weight 14-18 times is measured, subsequently enter water-filling distillation, and collect equivalent to medical material gross weight The distillate of 7-9 times of amount, obtains final product distillate medicinal water.
Preferably, the preparation method of pharmaceutical composition of the present invention, comprises the steps:
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 8-12 volumes according to every raw material medical material after mixing The ratio of part water, after soaking extraction 2-4 hours, 100 DEG C of reflux, extract, 2-4 hours collect volatile oil;It is filtrated to get simultaneously Aqueous extract and medicinal residues;
(2)The medicinal residues are taken, and the ratio of 8-12 parts by volume water are added according to every raw material medical material, are added water to cook extraction 1-3 hours, are filtrated to get aqueous extract;
(3)Merge above-mentioned aqueous extract, heating concentration, and add the volatilization oil and customary adjuvant of the collection, according to Conventional method is prepared into clinically acceptable oral liquid formulations, tablet, granule, capsule, mixture, pill or drop pill;
Relation of the relation of the weight portion and parts by volume for g/mL.
It is highly preferred that the preparation method of pharmaceutical composition of the present invention, comprises the steps:
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 12 parts by volume according to every raw material medical material after mixing The ratio of water, after soaking 3 hours, 100 DEG C of reflux, extract, 2.5 hours collect volatile oil;It is filtrated to get aqueous extract simultaneously And medicinal residues;
(2)The medicinal residues are taken, the ratio of 10 parts by volume water is added according to every raw material medical material, add water and decoct in 100 DEG C Extraction 1.5 hours is boiled, aqueous extract is filtrated to get;
(3)Merge the extracting solution, add and concentrate, and add the volatilization oil and customary adjuvant of the collection, according to normal Rule method is prepared into clinically acceptable oral liquid formulations, tablet, granule, capsule, mixture, pill or drop pill.
It is highly preferred that the preparation method of pharmaceutical composition of the present invention, comprises the steps:
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 12 parts by volume of distilled water by every weight portion crude drug after mixing, Volatile oil is extracted in immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours, collects volatile oil, and filtration obtains aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds 10 parts by volume of distilled water by every weight portion crude drug, added water to cook 1.5 hours, filtered Obtain aqueous extract;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1ml crude drugs containing 1g;
(4)When above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, solution relative density is 1.1-1.2, obtains medicinal liquid A;By medicinal liquid A Add ethanol to make its alcohol content more than 50%, stir, 4 DEG C stand 20-24 hours, filter, remove precipitate, obtain medical liquid B;Take Medical liquid B reclaims ethanol, medicinal liquid is suitably concentrated, a small amount of ethanol of residual of volatilizing, and obtains medicinal liquid C;Taking liquid C, adds to extract and collects Volatile oil and 1/4 medicinal liquid weight portion 80% sugar content syrup, mixing, fill, sterilizing obtain final product oral liquid formulations;Or,
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 12 parts by volume of distilled water by every weight portion crude drug after mixing, Volatile oil is extracted in immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours, collects volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds 10 parts by volume of distilled water by every weight portion crude drug, decocting in water 1.5 hours, filtration draws Aqueous extract;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)Clear paste of the solution relative density for 1.3-1.35 when above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, addition 1/3 are clear The starch of cream weight portion, mix homogeneously dry, pulverize, and are subsequently adding and extract the volatile oil collected, and mix, closed vexed 2 hours Afterwards, magnesium stearate, the Pulvis Talci of 1% medicated powder weight portion are separately added into, are mixed, tabletting, sugar coating or film-coat obtain final product tablet;Or Person,
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 12 parts by volume of distilled water by every weight portion crude drug after mixing, Volatile oil is extracted in immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours, collects volatile oil, and filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds 10 parts by volume of distilled water by every weight portion crude drug, decocting in water 1.5 hours, filtration draws Aqueous extract;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)Clear paste of the solution relative density for 1.3-1.35 when above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, addition 1/3 are clear The starch of cream weight portion, mix homogeneously dry, pulverize, and are subsequently adding and extract the volatile oil collected, and mix, closed vexed 2 hours Afterwards, loaded in capsule, obtain final product capsule;Or,
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 12 parts by volume of distilled water by every weight portion crude drug after mixing, Volatile oil is extracted in immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours, collects volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds 10 parts by volume of distilled water by every weight portion crude drug, decocting in water 1.5 hours, filtration draws Aqueous extract;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)Clear paste of the solution relative density for 1.3-1.35 when above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, addition 2/3 are clear The steaming Icing Sugar of cream weight portion, mix homogeneously dry, pulverize, and are subsequently adding and extract the volatile oil collected, and mix, closed vexed 2 hours Afterwards, subpackage, obtains final product granule;Or
(1)The Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, chrysanthemum are taken according to selected weight portion Flower, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 12 parts by volume of distilled water by every weight portion crude drug after mixing, Volatile oil is extracted in immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours, collects volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds distilled water 10L, decocting in water 1.5 hours, filtration to draw aqueous extract by 1kg crude drugs;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)When above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, solution relative density is 1.1-1.2, obtains medicinal liquid A;By medicinal liquid A Add ethanol to make its alcohol content more than 50%, stir, 4 DEG C of standing 20-24 hours filter, remove precipitate, obtain medical liquid B;Take Medical liquid B reclaims ethanol, medicinal liquid is suitably concentrated, a small amount of ethanol of residual of volatilizing, and obtains medicinal liquid C;Taking liquid C, adds to extract and collects Volatile oil and 1/4 medicinal liquid weight portion 80% sugar content syrup, mixing, fill, sterilizing obtain final product mixture;Or
According to selected weight portion take the Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, add customary adjuvant to make pill according to common process.
Present invention also offers the pharmaceutical composition that aforementioned pharmaceutical compositions or said method are prepared has in preparation Application in the medicine of heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity effect.
Pharmaceutical composition of the present invention based on Traditional Chinese medical theory, with Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, light Folium Bambusae, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae are used as medicine, and gained pharmaceutical composition has heat-clearing and toxic substances removing, antiinflammatory and enhancing Immunity, can be used for flu heatstroke, the laryngopharynx swelling and pain caused under affection due to external wind and heat, internal fever and immunologic function, liver-heat The treatment of the diseases such as the conjunctival congestion that causes, ophthalmalgia, facial acne, not only curative effect is prominent and toxicity is extremely low;Wherein,
Camellia nitidissima Chi, rich in tea polysaccharide, tea polyphenols, Total saponin, total flavones, tea pigment, protein, vitamin B1, B2, dimension life Various natural nutrition compositions such as plain C, Vitamin E, Folic Acid, fatty acid, B- carotene, increasing degree on energy effective control post-prandial glycemia Spend and improve glucose tolerance, and disorders of lipid metabolism can be improved, have obvious blood sugar decreasing effect;Simultaneously have vasodilator and Resisting fatigue, decreased heart rate and the effect of Bipolar blood pressure promote the biosynthesiss of DNA and protein and lipid, slake thirst and help produce saliva, evacuation of pus The effect such as detumescence, relieving sore throat and pain, antipyretic-antalgic;So that Camellia nitidissima Chi has preferable anticancer, reduces the work of cholesterol and lipoprotein With;
Flos Lonicerae, sweet in the mouth, slight bitter, delicate fragrance are pungent, tremble with fear;Return lung, stomach, the heart, large intestine channel;There is the work(of heat-clearing and toxic substances removing, dispelling wind and heat pathogens Effect;
Fructus Gardeniae, bitter, cold, purging intense heat relieving restlessness;With clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body effect;
Folium Nelumbinis, containing various alkaloids such as nuciferine, miltanthin and nuciferine and vitamin C, polysaccharide, with heat clearing away solution Poison, removing heat from blood, the effect of hemostasis;
Folium Mori, it is bitter in the mouth, sweet, cold in nature, act on blood pressure lowering, blood fat, antiinflammatory etc., be mainly used in dispelling wind and heat pathogens, lung heat clearing profit It is dry, liver heat removing and eyesight improving;
Herba Lophatheri, it is sweet light, cold, nontoxic, it is included into heart channel, kidney channel;With it is sweet it is light ooze profit, clear drop cold in nature is kind to lead the heart and small intestinal Fire is descending and inducing diuresis for treating stranguria syndrome effect;
Rhizoma Imperatae, with removing heat from blood, hemostasis, heat clearing away, diuretic effect, cures mainly pyreticosis polydipsia, spits blood, epistaxis, lung-heat dyspnea with rapid respiration, stomach Hot hiccup, gonorrhea, dysuria, edema, jaundice;
Flos Chrysanthemi, for anemopyretic cold, dizziness of having a headache, conjunctival congestion and swelling pain, eyes are dim-sighted;
Herba Menthae, it is pungent, cool, enter lung meridian, Liver Channel;With dispelling wind and heat pathogens, head clearing, sore-throat relieving rash, soothing the liver circulation of qi promoting effect;
Herba Rabdosiae Lophanthoidiss, it is sweet-bitter flavor, cool, cold, the effect of with clearing away heat-damp and promoting diuresis, cool the blood dissipate blood stasis;
Folium hydrangeae strigosae, has functions that resolving heat and reducing pathogenic fire, lung moistening, eliminating the phlegm, cough-relieving.
Pharmaceutical composition of the present invention, conventionally can be prepared into bag tea agent, distillate medicinal water, tablet, granule, capsule Agent, mixture, pill or drop pill, daily carrying and taking convenience.
Experimental example
Following experimental example and embodiment are further explained to the present invention.Following examples are only to optimal reality of the invention The description of mode is applied, does not have any restriction to the scope of the present invention.
The research 1, test objective of 1 pharmaceutical composition heat-clearing and toxic substances removing of the present invention of experimental example, antiinflammatory and enhancing immunity effect
By investigate pharmaceutical composition xylol of the present invention cause Mice Auricle inflammation, carrageenin cause mice foot swelling and The antiinflammatory action of mice granuloma induced by implantation of cotton pellets model;To being caused with chicken red blood cell, mice hemolytic antibody is generated and clearance in mice test is seen Examine its impact to immunologic function;Observation pharmaceutical composition of the present invention causes the impact of the antipyretic effect of heating rat to yeast;Together When Antimicrobial test method observe its to staphylococcus aureuses, bacillus pyocyaneus, escherichia coli, Candida albicans suppression Make and use;Pharmaceutical composition antiinflammatory of the present invention, the effect of antipyretic, antibacterial and the impact to immunologic function are investigated, is that its clinic is used Experimental basis are provided in heat-clearing and toxic substances removing.
2nd, experiment material
2.1 pharmaceutical compositions of the present invention:According to the bag tea agent that 1 methods described of embodiment is prepared, by Guangxi last course of a feast Primary Camellia nitidissima Chi development corporation, Ltd. provides, and Yao Zhang institutes of Guangxi Medical University laboratory voluntarily prepares Aqueous extracts, and per milliliter contains 2 grams Raw medicinal herbs, lot number:20130205.
2.2 tested material dosage and usage;
Gastric infusion:If high, medium and low(10.2g, 5.1g, 2.55g crude drug/kg)Three dosed administration groups(It is quite clinical same 40 times, 20 times, 10 times of consumption 0.255g raw medicinal herbs/kg), and positive drug and blank control group are set, institute is configured to distilled water Need concentration.
2.3 control drug
2.3.1 blank:Normal saline (NS)
2.3.2 positive control drug:Dexamethasone Zhejiang celestial being is according to Pharmacy stock Co., Ltd, lot number:120835;Biphenyl is double Ester:Zhejiang Wanbang Pharmaceutical Co., Ltd., lot number:A02130127;
Aspirin:Long-range medicine(China)Company limited, lot number:130907
2.4 laboratory animals and feeding environment
2.4.1 animal:
18~22g of Kunming mouse, male and female dual-purpose, SD 180~220g of rat, male are SPF levels, by Guangxi medical courses in general University Hospital Experimental Animal Center is provided.Laboratory animal production licence:SCXK osmanthus 2009-0002.Laboratory animal is using license Card:SYXK osmanthus 2009-0004.
2.4.2 rearing conditions:
25 ± 5 DEG C of room temperature, humidity 60 ± 20%.The feedstuff provided with Guangxi Medical University's medical experiment animal center is provided(Into Dividing has rice meal, Semen Maydis powder, fish flour, milk powder, Testa Tritici, multivitamin, cod-liver oil, egg and salt etc.).Freely ingest, drink water.
2.5 antibacterial
(bacterial strain 1-5 strains number are respectively staphylococcus aureuses:26001st, 26003,26112,000113,010221) wherein 1-3 strains are type strain, are purchased from Chinese pharmaceutical biological product inspection institute, and No. 4 No. 5 strains are clinical strain, Guangxi Medical University first Clinical laboratory of Affiliated Hospital Bacteriology Room isolation identification is provided.Bacillus pyocyaneus(Bacterial strain 1-5 strains number are respectively:10102、10104、 10211、030112、011011)Wherein 1-3 strains are type strain, are purchased from Chinese pharmaceutical biological product inspection institute, and No. 4 No. 5 strains are Clinical strain, clinical laboratory of No.1 Hospital Attached to Guangxi Medical Univ. Bacteriology Room isolation identification are provided.
Escherichia coli(Bacterial strain 1-5 strains number are respectively:44121、44113、44103、010101、010213)Wherein No. 1-3 Strain is type strain, is purchased from Chinese pharmaceutical biological product inspection institute, and No. 4 No. 5 strains are clinical strain, the first attached doctor of Guangxi Medical University Clinical laboratory of institute Bacteriology Room isolation identification is provided.
Candida albicans, wherein, bacterial strain 1 is type strain, and bacterial strain number is 2.538, is purchased from Chinese pharmaceutical biological product inspection Institute, the bacterial strain number of bacterial strain 2-5 are respectively 010414,010921,021021,020202 for clinical strain, Guangxi Medical University first Clinical laboratory of Affiliated Hospital Bacteriology Room isolation identification is provided.
3rd, laboratory observation index
According to the indication of pharmaceutical composition of the present invention, according to relevant pharmacodynamicss in new drug preclinical study guideline Requirement, carried out following observation index:
3.1 antibacterial tests
(List of references:[1] Qi Chen chief editor. herbal pharmacology research methodology, Beijing:People's Health Publisher, 1993, 275. [2] Li Yikui are edited. herbal pharmacology experimental methodology, Shanghai:Science and Technology of Shanghai publishing house, 1991,292.)
In-vitro antibacterial:To staphylococcus aureuses, bacillus pyocyaneus, escherichia coli, Candida albicans inhibitory action, it is every kind of 5 plants of bacterium.
3.2 antiinflammatory action
(List of references:Li Yikui is edited. herbal pharmacology experimental methodology. Shanghai:Science and Technology of Shanghai publishing house, 1991,298- 302.)
Observation xylol causes the impact of Mice Auricle inflammation;
The impact of the mice foot swelling inflammation caused by observation Carrageenan;
Effect of the observation to mice granuloma induced by implantation of cotton pellets.
The impact of 3.3 pairs of immunologic functions
(List of references:Li Yikui is edited, herbal pharmacology experimental methodology, Science and Technology of Shanghai publishing house, and 1991,295.)
Impact to Hemolysin formation;Clearance test.
3.4 antipyretic experiments
(List of references:Qi Chen edits, herbal pharmacology research methodology. Beijing:People's Health Publisher, 1993,297- 298.)
Refrigeration function to rat fever caused by yeast.
4th, statistical method
This data application SPSS18.0 softwares carry out statistical data analysis.Data withRepresent, multisample Compare between mean and analyzed using One-way ANOVA, with P<0.05 is that difference is statistically significant.
5th, experimental technique and result
5.1 in-vitro antibacterial
Pharmaceutical composition of the present invention is pressed into 1g in normal saline:The ratio mixed diluting of 4mL as initial concentration, in this base Normal saline proportional diluted is used to be 1 on plinth again:1、1:2、1:4、1:8、1:16、1:32、1:64 totally 7 drug level.Respectively will The medicine of above-mentioned 7 concentration presses 1 with culture medium:10 mixing, final concentration of the 1 of medicine in culture medium:10、1:20、1:40、1: 80、1:160、1:320、1:640, after mixing, autoclaving pours plate into, after standing 24h observations without bacterial growth, by equal Concentration, isodose are observed to every part of plate transferred species antibacterial, 37 DEG C of culture 24h.
Pharmaceutical composition of the present invention minimum inhibitory concentration (MIC) in vitro to staphylococcus aureuses is 1:320, to green pus Bacillus and colibacillary MIC are 1:10, to Candida albicans without obvious inhibitory action, the results are shown in Table 1-4.
Inhibitory action of the 1 JINHUA herbal tea of table to staphylococcus aureuses
"-" represents that asepsis growth "+" indicates bacteria growing
Inhibitory action of the 2 JINHUA herbal tea of table to bacillus pyocyaneus
"-" represents that asepsis growth "+" indicates bacteria growing
3 JINHUA herbal tea of table is to colibacillary inhibitory action
"-" represents that asepsis growth "+" indicates that bacterium has length
Inhibitory action of the 4 JINHUA herbal tea of table to Candida albicans
"-" represents that asepsis growth "+" indicates bacteria growing
5.2 xylol cause the impact of Mice Auricle inflammation
Kunming mice, SPF levels, male and female half and half, are divided into 5 groups by 50,10 per group.Group is respectively:Blank control group, Dexamethasone matched group, low dose group (2.55g/kg), middle dose group (5.lg/kg), high dose group (10.2g/kg).Gavage is given Relative medicine is given, once a day, for three days on end.3 hours after 3rd day first administration, carry out second administration.It is administered for second After 1h, 50 μ L of dimethylbenzene are smeared to the right outer auricle of mice and causes acute inflammation model.After applying dimethylbenzene 1h, take off cervical vertebra and put to death, use Card punch lays auricle in left and right ear same position, weighs, the results are shown in Table 5.
Auris dextra quality after ear swelling=cause inflammation-do not cause scorching left auricle quality
Inhibitory rate of intumesce=(The average swelling of the matched group-average swelling of medication group)The average swelling of/matched group × 100%;
Compare with blank group, each administration group ear swelling degree has decline, the results are shown in Table 5.
Impact of the 5 JINHUA herbal tea of table to mice ear degree
* compared with blank, P < 0.05, # compared with dexamethasone, P < 0.05
5.2 Carrageenans cause the impact of mice foot swelling
Kunming mice, SPF levels, male and female half and half, are divided into 5 groups by 50,10 per group.Group is respectively:Blank control group, Dexamethasone matched group, low dose group (2.55g/kg), middle dose group (5.lg/kg), high dose group (10.2g/kg).Gavage is given Relative medicine is given, once a day, for three days on end.After 3rd day first administration 1h, carrageenin (1%) is injected to mice left foot toe, often Only 50 μ L.3 hours after 3rd day first administration, carry out second administration.After second administration 1h, take off cervical vertebra and put to death, by mice Left and right toes are cut, and weigh.The results are shown in Table 6.
Compared with blank group, each administration group toes swelling has decline, the results are shown in Table 6.
Impact of the 6 JINHUA herbal tea of table to mice toes swelling
* compared with blank group, P < 0.05., # compared with dexamethasone, P < 0.05
The impact that 5.3 pairs of mice granuloma induced by implantation of cotton pellets are formed
KM mices 50 are taken, 10 groups is randomly divided into, respectively blank control group, positive controls(Dexamethasone tablet 5mg/ kg)With sample low dose group (2.55g/kg), middle dose group (5.lg/kg), high dose group (10.2g/kg), 10 per group.
Aseptically, the sterilizing cotton balls of a 10mg, postoperative daily gavage are implanted in mouse armpit portion subcutaneous operation It is administered once, after continuous 12 days, puts to death mice, peel off cotton balls, claim weight in wet base, be re-weighed after 60 DEG C of drying in oven, calculates mice and put down Per the granuloma quality (mg) that the training of 10g weights is raw.The results are shown in Table 7.
Granulation dry weight=implantation granuloma induced by implantation of cotton pellets weight-implantation cotton balls weight;
Suppression ratio %-(Blank group granuloma weight-administration group granuloma weight)/ blank group granuloma weight.
Compared with blank group, each administration group suppression ratio has rising, the results are shown in Table 7.
7 JINHUA herbal tea of table is formed on mice granuloma induced by implantation of cotton pellets to be affected
Note:* * P < 0.01, * P < 0.05 compared with blank group;Compared with loose group of dexamethasone, #P < 0.05
The impact that 5.4 pairs of chicken red blood cells cause mice hemolytic antibody to generate
The mice of 18~22g of body weight is taken, male and female half and half are randomly divided into:NS groups, Dexamethasone group (5mg/kg), the present invention The high, medium and low dosage group of pharmaceutical composition(10.2nd, 5.1,2.55g crude drugs/kg), gastric infusion, once a day, continuous 8 days.In giving The 4th day after medicine, every mouse peritoneal injection 3:5(RBC hematocrits:NS, V/V)Chicken erythrocyte suspension 0.2mL.After last dose 24h, Each mice takes blood, separates serum, takes 50 μ L of serum and be diluted to 5mL, take dilute serum 2mL and 50mL/L chicken red blood cell suspensions, 100mL/L complements 1mL mixes, after being incubated 30min in 37 DEG C of water-baths, in 0 DEG C of refrigerator, stopped reaction.Centrifugation, take supernatant in OD values are surveyed at 540nm, with half hemolysis value (HC50) reflection hemolysin level.
Half hemolysis value (HC50Light absorption value × extension rate during)=sample light absorption value/chicken red blood cell HD50.
As a result show, the animal Jing after chicken red blood cell immunity, the anti-chicken red blood cell antibody of animal generation of high dose group is obvious Increase, it is seen that pharmaceutical composition of the present invention enhancing immunity in doses, the results are shown in Table 8.
8 JINHUA herbal tea of table causes the impact that mice hemolytic antibody is generated to sheep red blood cell (SRBC)
Compared with physiological saline group:* P < 0.05, * * P < 0.01
The impact of 5.5 pairs of mice phagocytic functions(Clearance test)
50 kunming mices, SPF levels, body weight 20 ± 2g mices, male and female half and half are randomly divided into 5 groups:NS matched groups, biphenyl Double fat groups (200mg/kg), the high, medium and low dosage group of pharmaceutical composition of the present invention (10.2,5.1,2.55g crude drugs/kg).Gavage is given Medicine, once a day, continuous 7 days.After last dose 24h, mouse mainline burnt black ink 0.1mL/10g, 2 after injection ink Minute, take within 10 minutes 50 μ L of blood respectively, be placed in 0.1%Na2CO3In solution, 3mL0.1% is dissolved in 50 μ L of Normal Mouse Serum Na2CO3Solution school zero, surveys OD values, at 675nm on spectrophotometer by formula K=log (OD2/OD10)/t10- t2=1/8log (OD2/OD10) calculate phagocytic index.
As a result show, pharmaceutical composition high dose group of the present invention can improve phagocyte imdex, have aobvious with Normal group Sex differernce is write, 9 are the results are shown in Table.
Impact of the 9 JINHUA herbal tea of table to mice phagocytic function
Compare with Normal group:* P < 0.05
The impact of rat fever caused by 5.6 pairs of yeast
It is prepared by sterilised yeast suspension:Take dry yeast 20g to be placed in mortar, add water and be ground to uniform suspension, be settled to 100mL, obtains final product 20% yeast suspension.Face used time Fresh.50 SD rats will be selected and be randomly divided into 5 groups, 10 per group: Matched group (normal saline 10mL/kg);Positive controls(Aspirin 0.1g/kg):Pharmaceutical composition of the present invention is high, medium and low Dosage group(10.2g crude drugs/kg, 5.1g crude drug/kg, 2.55g crude drug/kg), after packet, start administration, once a day, continuous 3 My god.1 hour every 20% sterilised yeast suspension 10mL/kg of rat back subcutaneous injection after last dose.Then in giving yeast suspension 2,4,6,8 hours each survey rat temperatures once, compare the change of different time body temperature afterwards.
As a result show, the body temperature that the middle and high dosage group of pharmaceutical composition of the present invention causes to yeast is raised certain solution Heat effect, is shown in Table 10.
Impact of the 10 JINHUA herbal tea of table to rat fever caused by yeast
Compare with matched group:* P < 0.05, * * P < 0.01.
6th, experiment conclusion
Result of study shows, pharmaceutical composition of the present invention minimum inhibitory concentration (MIC) in vitro to staphylococcus aureuses For 1:320, it is 1 to bacillus pyocyaneus and colibacillary MIC:10, illustrate to staphylococcus aureuses, bacillus pyocyaneus and large intestine bar Bacterium has certain inhibitory action, but to Candida albicans without obvious inhibitory action;Pharmaceutical composition of the present invention significantly can subtract Auricle edema and the mice foot swelling caused by carrageenin caused by light proinflammatory agent dimethylbenzene, caused by illustrating xylol proinflammatory agent Mice auricle swelling, the mice toes swelling caused by carrageenin and granuloma induced by implantation of cotton pellets be formed with obvious inhibitory action, table Bright pharmaceutical composition of the present invention has obvious antiinflammatory action;It is anti-that pharmaceutical composition of the present invention can promote mice to produce anti-chicken red blood cell The generation of body, and phagocyte imdex is improved, show which has certain enhancing immunity effect, wherein acting on high dose group It is stronger;The body temperature caused to yeast is raised stronger refrigeration function.
The toxicity research of 2 pharmaceutical composition of the present invention of experimental example
Purpose:Pharmaceutical composition maximum tolerated dose of the present invention is determined, to understand its toxicity size and Drug safety.
Method:With Kunming mouse as object of study, the maximum that gavage (i.g.) gives pharmaceutical composition of the present invention is observed Dosis tolerata.
1st, material
1.1 laboratory animal
SPF level KM mices, male and female half and half, 20 ± 2g of body weight are provided by Guangxi Medical University's Experimental Animal Center.Animal is raised Support in the feeding environment for meeting medical experiment animal environment facility requirements.Rearing conditions:25 ± 2 DEG C of room temperature, humidity 60 ± 20%.Laboratory animal production licence:SCXK osmanthus 2009-0002.Laboratory animal uses licence:SYXK osmanthus 2009-0004.
1.2 test medicine
Pharmaceutical composition of the present invention:(According to the bag tea agent that 1 methods described of embodiment is prepared):It is former by Guangxi last course of a feast Raw Camellia nitidissima Chi development corporation, Ltd. provides, and lot number 20130205 prepares Aqueous extracts by pharmaceutical college of Guangxi Medical University laboratory, often Milliliter contains 2 grams of raw medicinal herbs.
2nd, method and result
Method:20 mices are taken, male and female half and half are randomly divided into pharmaceutical composition group of the present invention and blank control group, per group 10.It is administered with pharmaceutical composition i.g. of the present invention, each dosage is 60gkg-1, 4 times a day, is administered daily accumulated dose For 240gkg-1, volume is administered every time is 30mLkg-1.Blank control group such as gives at the normal saline (NS) of capacity.Give After medicine, the continuous observations of Ze 14 days, record animal toxicity response situation and whether there is animal dead.
As a result:The no abnormality seen such as the 2nd~14 day animal spirit, activity, defecation after administration, body weight increase are right with blank According to a group no significant difference, laboratory animal is without death.The maximum tolerated dose of mice is 240gkg-1·d-1, Mouse Weight changes to be seen Table 11.
Impact of the 11 JINHUA herbal tea of table to Mouse Weight
Note:JINHUA herbal tea group is compared with blank control group:P > 0.05
3rd, conclusion
Mice maximum tolerated dose is 240gkg-1·d-1, daily plan consumption clinical equivalent to pharmaceutical composition people of the present invention (0.255/kg)941 times.Obvious toxic reaction is had no in test, it is thus regarded that this product toxicity is relatively low.
Specific embodiment
The preparation of 1 medicinal tea of embodiment:
Camellia nitidissima Chi 1500g Flos Lonicerae 1500g Fructus Gardeniae 2400g Folium Nelumbinis 3000g
Folium Mori 3000g Herba Lophatheri 3000g Rhizoma Imperatae 3000g Flos Chrysanthemi 2400g
Herba Menthae 2400g Herba Rabdosiae Lophanthoidiss 3000g Folium hydrangeae strigosae 4800g
Above-mentioned 11 taste, mixes after crushing or being ground into coarse powder after mixing respectively, and sterilizing, subpackage obtain final product teabag finished product 5000 Bag, per bag of 6g containing crude drug.
The preparation of 2 distillate medicinal water of embodiment:
Camellia nitidissima Chi 2kg Flos Lonicerae 2.5kg Fructus Gardeniae 3kg Folium Nelumbinis 4kg
Folium Mori 4kg Herba Lophatheri 4kg Rhizoma Imperatae 5kg Flos Chrysanthemi 5kg
Herba Menthae 5kg Herba Rabdosiae Lophanthoidiss 4kg Folium hydrangeae strigosae 6kg
Above-mentioned 11 taste, mixes after crushing or being ground into coarse powder, add the distillation water logging of 16 times of medical material weight after mixing respectively The bubble distillation of laggard water-filling in 3 hours, collects the distillate equivalent to 8 times of medical material weight, and sterilizing, subpackage obtain final product distillate medicinal water finished product 1000 Bottle, per bottled amount 360mL, 45.5g containing crude drug.
The preparation of 3 tablet of embodiment:
Camellia nitidissima Chi 1000g Flos Lonicerae 1200g Fructus Gardeniae 2000g Folium Nelumbinis 2500g
Folium Mori 2500g Herba Lophatheri 2500g Rhizoma Imperatae 2500g Flos Chrysanthemi 2000g
Herba Menthae 1800g Herba Rabdosiae Lophanthoidiss 3000g Folium hydrangeae strigosae 5000g
Above-mentioned 11 taste, is ground into coarse powder, makes tablet as follows:
(1)Distilled water 12L, immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours is added to extract volatile oil by 1kg crude drugs, Collect volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds distilled water 10L, decocting in water 1.5 hours, filtration to draw aqueous extract by 1kg crude drugs;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)Clear paste of the solution relative density for 1.3-1.35 when above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, addition 1/3 are clear The starch of cream weight portion, mix homogeneously dry, pulverize, and are subsequently adding and extract the volatile oil collected, and mix, closed vexed 2 hours Afterwards, magnesium stearate, the Pulvis Talci of 1% medicated powder weight portion are separately added into, are mixed, tabletting, sugar coating or film-coat draw tablet 5000, per piece 5.2g containing crude drug.The preparation of 4 capsule of embodiment:
Camellia nitidissima Chi 2kg Flos Lonicerae 1.8kg Fructus Gardeniae 2.5kg Folium Nelumbinis 3kg
Folium Mori 3.5kg Herba Lophatheri 3kg Rhizoma Imperatae 4kg Flos Chrysanthemi 3kg
Herba Menthae 2.5kg Herba Rabdosiae Lophanthoidiss 5kg Folium hydrangeae strigosae 6kg
Above-mentioned 11 taste, is ground into coarse powder, makes capsule as follows:
(1)Distilled water 12L, immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours is added to extract volatile oil by 1kg crude drugs, Collect volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds distilled water 10L, decocting in water 1.5 hours, filtration to draw aqueous extract by 1kg crude drugs;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)Clear paste of the solution relative density for 1.3-1.35 when above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, addition 1/3 are clear The starch of cream weight portion, mix homogeneously dry, pulverize, and are subsequently adding and extract the volatile oil collected, and mix, closed vexed 2 hours Afterwards, it is sub-packed in capsule, draws capsule in blocks 6000, per 6.05g containing crude drug.
The preparation of 5 granule of embodiment:
Camellia nitidissima Chi 1.6kg Flos Lonicerae 2kg Fructus Gardeniae 3kg Folium Nelumbinis 3.5kg
Folium Mori 3kg Herba Lophatheri 3kg Rhizoma Imperatae 5kg Flos Chrysanthemi 4kg
Herba Menthae 3.5kg Herba Rabdosiae Lophanthoidiss 5kg Folium hydrangeae strigosae 5kg
Above-mentioned 11 taste, is ground into coarse powder, makes granule as follows:
(1)Distilled water 12L, immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours is added to extract volatile oil by 1kg crude drugs, Collect volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds distilled water 10L, decocting in water 1.5 hours, filtration to draw aqueous extract by 1kg crude drugs;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)Clear paste of the solution relative density for 1.3-1.35 when above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, addition 2/3 are clear The steaming Icing Sugar of cream weight portion, mix homogeneously dry, pulverize, and are subsequently adding and extract the volatile oil collected, and mix, closed vexed 2 hours Afterwards, subpackage, draws 3800 bags in blocks(10g/ bags), per 10.16g containing crude drug.
The preparation of 6 mixture of embodiment:
Camellia nitidissima Chi 5kg Flos Lonicerae 4kg Fructus Gardeniae 6kg Folium Nelumbinis 10kg
Folium Mori 12kg Herba Lophatheri 8kg Rhizoma Imperatae 10kg Flos Chrysanthemi 8kg
Herba Menthae 6kg Herba Rabdosiae Lophanthoidiss 12kg Folium hydrangeae strigosae 20kg
Above-mentioned 11 taste, is ground into coarse powder, makes mixture as follows:
(1)Distilled water 12L, immersion 100 DEG C of post-heating distillation 2.5 hours in 3 hours is added to extract volatile oil by 1kg crude drugs, Collect volatile oil;Filtration draws aqueous extract and medicinal residues;
(2)Above-mentioned medicinal residues are extracted, adds distilled water 10L, decocting in water 1.5 hours, filtration to draw aqueous extract by 1kg crude drugs;
(3)Merge above aqueous extract twice, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4)When above-mentioned concentrated solution is continued to be concentrated into 70 DEG C, solution relative density is 1.1-1.2, obtains medicinal liquid A;By medicinal liquid A Add ethanol to make its alcohol content more than 50%, stir, 4 DEG C of standing 20-24 hours filter, remove precipitate, obtain medical liquid B;Take Medical liquid B reclaims ethanol, medicinal liquid is suitably concentrated, a small amount of ethanol of residual of volatilizing, and obtains medicinal liquid C;Taking liquid C, adds to extract and collects Volatile oil and 1/4 medicinal liquid weight portion 80% sugar content syrup, mixing, fill, sterilizing get product 10000, mixture (10mL/), per a 10.1g containing crude drug.
The preparation of 7 pill of embodiment:
The present embodiment described pharmaceutical composition prescription is same as Example 3.According to selected weight portion take the Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing Close, add customary adjuvant to make pill according to common process.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right For those of ordinary skill in the art, can also make on the basis of the above description other multi-forms change or Change.There is no need to be exhaustive to all of embodiment.And thus it is extended obvious change or Among changing still in the protection domain of the invention.

Claims (10)

1. it is a kind of with heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity effect pharmaceutical composition, it is characterised in that said composition Crude drug is consisted of:
2. it is according to claim 1 with heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity effect pharmaceutical composition, its feature It is that the crude drug of said composition is consisted of:
3. it is according to claim 2 with heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity effect pharmaceutical composition, its feature It is that the crude drug of said composition is consisted of:
4. according to any one of claim 1-3 with heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity effect drug regimen Thing, it is characterised in that described pharmaceutical composition adds customary adjuvant, according to common process, makes clinically acceptable bag bubble Medicinal tea, distillate medicinal water, oral liquid formulations, tablet, granule, capsule, mixture, pill or drop pill.
5. a kind of preparation method of any one of claim 1-4 described pharmaceutical composition, it is characterised in that include according to selected Weight portion takes the Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, Mix after crushing or crush after mixing, and selectivity addition customary adjuvant, according to common process, bag tea agent is obtained.
6. a kind of preparation method of any one of claim 1-4 described pharmaceutical composition, it is characterised in that include according to selected Weight portion takes the Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, Herba Menthae, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, Mix after crushing or crush after mixing, and add the distilled water immersion 2-4 hours of medical material gross weight 14-18 times of weight amount, subsequently Enter water-filling distillation, and collect the distillate equivalent to medical material gross weight 7-9 times, obtain final product distillate medicinal water.
7. a kind of preparation method of any one of claim 1-4 described pharmaceutical composition, it is characterised in that comprise the steps:
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add 8-12 parts by volume water according to every raw material medical material after mixing Ratio, after soaking extraction 2-4 hours, 100 DEG C of reflux, extract, 2-4 hours collect volatile oil;It is filtrated to get water extraction simultaneously Liquid and medicinal residues;
(2) above-mentioned medicinal residues are taken, the ratio of 8-12 parts by volume water is added according to every raw material medical material, add water to cook extraction 1-3 Hour, it is filtrated to get aqueous extract;
(3) merge above-mentioned aqueous extract, heating concentration, and add the volatile oil and customary adjuvant of above-mentioned collection, according to conventional side Method is prepared into clinically acceptable oral liquid formulations, tablet, granule, capsule, mixture, pill or drop pill;
Relation of the relation of the weight portion and parts by volume for g/mL.
8. the preparation method of pharmaceutical composition according to claim 7, it is characterised in that comprise the steps:
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush, add the ratio of 12 parts by volume water according to every raw material medical material after mixing Example, after soaking 3 hours, 100 DEG C of reflux, extract, 2.5 hours collect volatile oil;It is filtrated to get aqueous extract and medicine simultaneously Slag;
(2) above-mentioned medicinal residues are taken, the ratio of 10 parts by volume water is added according to every raw material medical material, add water to cook extraction 1.5 little When, it is filtrated to get aqueous extract;
(3) merge above-mentioned aqueous extract, heating concentration, and add the volatile oil and customary adjuvant of above-mentioned collection, according to conventional side Method is prepared into clinically acceptable oral liquid formulations, tablet, granule, capsule, mixture, pill or drop pill.
9. the preparation method of the pharmaceutical composition according to claim 7 or 8, it is characterised in that comprise the steps:
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, add 12 parts by volume of distilled water, immersion 3 by every weight portion crude drug After hour, heat 100 DEG C of distillations and extract volatile oil in 2.5 hours, collect volatile oil, filtration obtains aqueous extract and medicinal residues;
(2) above-mentioned medicinal residues are taken, adds 10 parts by volume of distilled water by every weight portion crude drug, added water to cook 1.5 hours, filtration obtains water Extracting solution;
(3) merge above-mentioned aqueous extract, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4) when continuing to be concentrated into 70 DEG C by above-mentioned concentrated solution, solution relative density is 1.1-1.2, obtains medicinal liquid A;Medicinal liquid A is added Ethanol makes its alcohol content more than 50%, stirs, 4 DEG C of standing 20-24 hours, filters, removes precipitate, obtain medical liquid B;Taking liquid B reclaims ethanol, medicinal liquid is suitably concentrated, a small amount of ethanol of residual of volatilizing, and obtains medicinal liquid C;Taking liquid C, adds waving for above-mentioned collection The syrup of 80% sugar content of hair oil and 1/4 medicinal liquid weight portion, mixing, fill, sterilizing obtain final product oral liquid formulations;Or,
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, add 12 parts by volume of distilled water, immersion 3 by every weight portion crude drug Volatile oil is extracted in the distillation 2.5 hours of 100 DEG C of hour post-heating, collects volatile oil;Filtration draws aqueous extract and medicinal residues;
(2) above-mentioned medicinal residues are taken, adds 10 parts by volume of distilled water, decocting in water 1.5 hours, filtration to draw water extraction by every weight portion crude drug Liquid;
(3) merge above-mentioned aqueous extract, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4) clear paste of the solution relative density for 1.3-1.35 when continuing to be concentrated into 70 DEG C by above-mentioned concentrated solution, adds 1/3 clear paste weight The starch of amount part, mix homogeneously dry, pulverize, and be subsequently adding the volatile oil of above-mentioned collection, mix, after closed vexed 2 hours, point Magnesium stearate, the Pulvis Talci of 1% medicated powder weight portion are not added, is mixed, tabletting, sugar coating or film-coat obtain final product tablet;Or,
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, add 12 parts by volume of distilled water, immersion 3 by every weight portion crude drug Volatile oil is extracted in the distillation 2.5 hours of 100 DEG C of hour post-heating, collects volatile oil, and filtration draws aqueous extract and medicinal residues;
(2) above-mentioned medicinal residues are taken, adds 10 parts by volume of distilled water, decocting in water 1.5 hours, filtration to draw water extraction by every weight portion crude drug Liquid;
(3) merge above-mentioned aqueous extract, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4) clear paste of the solution relative density for 1.3-1.35 when continuing to be concentrated into 70 DEG C by above-mentioned concentrated solution, adds 1/3 clear paste weight The starch of amount part, mix homogeneously dry, pulverize, and be subsequently adding the volatile oil of above-mentioned collection, mix, after closed vexed 2 hours, dress In capsule, capsule is obtained final product;Or,
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, add 12 parts by volume of distilled water, immersion 3 by every weight portion crude drug Volatile oil is extracted in the distillation 2.5 hours of 100 DEG C of hour post-heating, collects volatile oil;Filtration draws aqueous extract and medicinal residues;
(2) above-mentioned medicinal residues are taken, adds 10 parts by volume of distilled water, decocting in water 1.5 hours, filtration to draw water extraction by every weight portion crude drug Liquid;
(3) merge above-mentioned aqueous extract, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4) clear paste of the solution relative density for 1.3-1.35 when continuing to be concentrated into 70 DEG C by above-mentioned concentrated solution, adds 2/3 clear paste weight The steaming Icing Sugar of amount part, mix homogeneously dry, pulverize, and be subsequently adding the volatile oil of above-mentioned collection, mix, after closed vexed 2 hours, Subpackage, obtains final product granule;Or
(1) Camellia nitidissima Chi, Flos Lonicerae, Fructus Gardeniae, Folium Nelumbinis, Folium Mori, Herba Lophatheri, Rhizoma Imperatae, Flos Chrysanthemi, thin is taken according to selected weight portion Lotus, Herba Rabdosiae Lophanthoidiss and Folium hydrangeae strigosae, mix after crushing or crush after mixing, add 12 parts by volume of distilled water, immersion 3 by every weight portion crude drug Volatile oil is extracted in the distillation 2.5 hours of 100 DEG C of hour post-heating, collects volatile oil;Filtration draws aqueous extract and medicinal residues;
(2) above-mentioned medicinal residues are taken, adds distilled water 10L, decocting in water 1.5 hours, filtration to draw aqueous extract by 1kg crude drugs;
(3) merge above-mentioned aqueous extract, Rotary Evaporators are concentrated into 1mL crude drugs containing 1g;
(4) when continuing to be concentrated into 70 DEG C by above-mentioned concentrated solution, solution relative density is 1.1-1.2, obtains medicinal liquid A;Medicinal liquid A is added Ethanol makes its alcohol content more than 50%, stirs, 4 DEG C of standing 20-24 hours, filters, removes precipitate, obtain medical liquid B;Taking liquid B reclaims ethanol, medicinal liquid is suitably concentrated, a small amount of ethanol of residual of volatilizing, and obtains medicinal liquid C;Taking liquid C, adds waving for above-mentioned collection The syrup of 80% sugar content of hair oil and 1/4 medicinal liquid weight portion, mixing, fill, sterilizing obtain final product mixture.
10. any one of claim 1-4 described pharmaceutical composition or preparation method described in any one of claim 5-9 are prepared Pharmaceutical composition prepare with heat-clearing and toxic substances removing, antiinflammatory and enhancing immunity effect medicine in application.
CN201410134543.2A 2014-04-03 2014-04-03 Pharmaceutical composition with effects of clearing away heat and toxic materials, resisting inflammations and boosting immunity Expired - Fee Related CN103893593B (en)

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